LOCUS       DAOPJL010000001       101066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_166_length_101066_cov_7.951877, whole genome shotgun sequence.
ACCESSION   DAOPJL010000001 DAOPJL010000000
VERSION     DAOPJL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 101066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 101066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101066
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_101066_cov_7.951877"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1395)
                     /locus_tag="PLP02_00005"
     CDS             complement(79..1395)
                     /locus_tag="PLP02_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPF41880.1"
                     /translation="MKKKGLIFILLVTLVFVFAGCDFFSISSTTTTERTFLTSSTTNT
                     NTTTTTIDTDRLVSEVYAMLYNDLYEQIREEVIGDITEERFQEIYDQIITQIYGDLDE
                     GTLSLEAVSLAEQVLRVATDEASSIIGVSNYSSSGLLQSVGSGVIYKHVGTMYYVVTN
                     HHVVEDDATDDGITNDPRIRLGDGTEIAATILGYDETTDLAVLTFVTDRTLNVASFGD
                     SSDLATGTVVLAVGNPDGYEFFDSVTMGIVSGTRRYFDTDNDGITEMYFSFIQHDASI
                     NSGNSGGALFNLDGEVIGINSIKISSSEIEGMGFAIPISLVAAVCADIELYGFSKQTP
                     VLGVQIADIATVTPEEFAQEGITIPEGIEAGYYVIAVMSGSSVDGYVQAGDIITQIGD
                     IVISNKTETSKDFYELYHVGDVISIVVYRNGSYITLENIELLSKTS"
     gene            complement(1478..2095)
                     /locus_tag="PLP02_00010"
     CDS             complement(1478..2095)
                     /locus_tag="PLP02_00010"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPF41881.1"
                     /translation="MSRKIKIFAGPNNYDFSRLYFEEADEFIVGVDSGLKYLVENNIM
                     IDLAIGDFDSLDPLFFDSVKQKSRKLITLSKEKSMTDLAYAIDYLYNNTDYTSLEIYG
                     GIGGRIDHLLANINLLKRFDLSFRDNQHHIFVLGKGKHHIDNYHRYISFFAIEDCYNL
                     SLTGFKYSLNNYYLSTSDSLCVSNEGSGEVGFSKGRLLVVMSDEK"
     gene            complement(2088..2729)
                     /gene="rpe"
                     /locus_tag="PLP02_00015"
     CDS             complement(2088..2729)
                     /gene="rpe"
                     /locus_tag="PLP02_00015"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPF41882.1"
                     /translation="MICAPSFLTADFSQLETEIVSLKKAKWLHFDVMDGIFVPNLTYG
                     EEMLDRVKAFSNQFFDCHLMIANPLERYRDFIDAGADMITFHYEAAKEKSANLIAKIK
                     GRGCLSGMSIKPATKADLLDPFLKDLDLVLVMSVEPGKGGQEFMPEALDKIRYYADQR
                     SKNNYHFLIEVDGGINDKTAAMAKTSGADIVVAGSYIINQKDREKAIGVLENV"
     gene            complement(2745..3611)
                     /gene="rsgA"
                     /locus_tag="PLP02_00020"
     CDS             complement(2745..3611)
                     /gene="rsgA"
                     /locus_tag="PLP02_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPF41883.1"
                     /translation="MEKGRIIKLIGGWYTVAGCDGKRRDVKPLGIFRYQNERPKVGDI
                     VSFDDESILSVETRTNDLVRPSICNVDQVLLIMSAKRPEFSLQLLDRFLILILNSRIT
                     PAIIITKTDLMTKDERETLEKTAEYYRSFFPTVFVSNKTNEGLEEIKKLTKGKTNVLA
                     GQTGAGKSSLLNSLNPTLELKTDDISEALGRGKHTTRHVELIEFGDGWIADTPGFSKL
                     DFIDIEPDSLQNLYPDFVNLSEGCRFSGCRHLKEPDCKVKEAVEAGSILKERYDNYCR
                     FYAEIKAIKPKY"
     gene            complement(3687..4232)
                     /locus_tag="PLP02_00025"
     CDS             complement(3687..4232)
                     /locus_tag="PLP02_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPF41884.1"
                     /translation="MMELKGSKTEQNLQQAFAGESMARNKYTFYASKAKKEGYVQIAN
                     FFLETAANEKEHAEVWFKLLHDGGIPATEANLEDGAAGEHYEWTDMYKGFAEVAKQEG
                     FTKIAYLFEAVAAIEKEHEKRYLALLASIKDQRIFESDNDSEIWICSNCGHIHVGKKA
                     PKVCPVCDHDQSYFQRYVKNY"
     gene            complement(4262..5407)
                     /locus_tag="PLP02_00030"
     CDS             complement(4262..5407)
                     /locus_tag="PLP02_00030"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPF41885.1"
                     /translation="MDANKKIKRIAILTGGGDCSGLNAVIRAAVRTAILKYGYEVIGY
                     MGGYYGLFKDDYIDLTLSAVTGIFHQGGTILKMSNRDNLFNYRTMDSNGNYVYLDVSD
                     IAIENLRKNGVDAIIIIGGDGTLTSARDFSRKGVNVVGIPKTIDNDVPDTEITFGYRT
                     ALDHIMLSLDALHTTAFSHDRVMILEVMGRHAGWLALEGGIAGSADVILIPEIPYDIK
                     KVAKQIIDRYNYGSKFSIICVSEGAKPIDGELTIRTIDNKSPDSVKLGGIGARIASQL
                     ETLIQPITGQEIRSTNLGYIQRGGVTDTFDRVLGTKYGSHAVDMIANGEFGNMVTIMN
                     DKMTMVPIEDVVGHGKTGETSRGGARLVDPEGDLVQAARNIGISFGD"
     gene            complement(5479..6510)
                     /gene="rlmN"
                     /locus_tag="PLP02_00035"
     CDS             complement(5479..6510)
                     /gene="rlmN"
                     /locus_tag="PLP02_00035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPF41886.1"
                     /translation="MEKTNLYGLSLQELGIMLSEAGFKQYNGRQIFEWMYQKNIRDFG
                     LMTNLSLKLREYLSDHFGFGLPGIATIQNSSDGTRKYLFAMNDGSLIETVLMTHDYGY
                     SVCVSSQVGCNMKCAFCASGLKNKIRNLDTAELVGQVIMAGLEAKVRITNVVVMGTGE
                     PFDNYENVIKFIGIINESKGLAIGQRHITVSTSGIVPMIRRYAEEPIRSNLAVSLHAS
                     DDTLRNRLMPINRKYPVAEVIRACKDYFEKTSRRVTFEYILLKGVNDSLEQANKLADL
                     LRDVNCYVNLIPYNPVDEFEFDPSGHDATSIFLDCLMKRKINVTMRKEQGGDIDAACG
                     QLRMKSKTK"
     gene            complement(6645..9014)
                     /gene="pheT"
                     /locus_tag="PLP02_00040"
     CDS             complement(6645..9014)
                     /gene="pheT"
                     /locus_tag="PLP02_00040"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPF41887.1"
                     /translation="MLLSMNWLKDYININIDKDELVRKLNLMSAEVEGLEKMVEATNL
                     AIGFVETCVMHPDSDHLHVCVVDTGNEKLDIICGAPNVEAGQKVIVALPGANLPGGLK
                     IKKAKIRGIESNGMICSLSELGIDHKYHQEDGIHVLSSDAPLGGDPLAWLSLDDYILN
                     LDLTPNRADLLSHLGVAYELGAVLNESVNVSNPDPEEDQEKNPVCVFTETSDCESYYA
                     RVIKDVKVGLSPRWMQSRLIACGIRPINNVVDITNYVMLEYGQPLHAFDYDKLVSGQI
                     LVRNAKEGELIVTLDGKKRELDPADVVITDGNVPIALAGVMGGLETEIDDNTTTILLE
                     SATFSPYAVRKTSKRLALQSESSNRFERGLDPARTRLAANRAAELFREYCGGRILRGI
                     NYFDVNDAGDTVILTTMAKIESVTGKRYTEKELEDVFERLNFRAKITDDDITVYAPSR
                     RQDIKTYQDVIEEIVRINGYNNIPMTLPKTITTGTLTDIQKQRRMLRSFMLDLGFDEV
                     TTYTLTNEHNARMFDDSNKKVIKILNPISEERASLRHSLLPSLISVLKYNKDHKQDEA
                     CLFEIGKGYFDEGEEEFLSGIMNGSYRMSKWQHDQANIDFYLAKGIIIAILKKLGLED
                     IDFQIPDKKLPGFHPGVVANVTSKGKKIGVLGRFHPELEHSEEIGETYAFELNLGRIF
                     GCGNKNIVMKPLSKYPSVSRDLAVIVDKNLPAGDLVETIRKMNMKSVRIVEIFDRYEG
                     EKLGQDKKSIALSLVFQDDGKTLSTTDVDNFVNKIVGELAKKCQATLRE"
     gene            complement(9027..10055)
                     /gene="pheS"
                     /locus_tag="PLP02_00045"
     CDS             complement(9027..10055)
                     /gene="pheS"
                     /locus_tag="PLP02_00045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014092613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPF41888.1"
                     /translation="MKEQLAKIVAEAKSAAEGIKTPAELGDLKAAFLGKKSEFNTMMQ
                     QMKDLTPEERPAFGGIINAAKSEVTAFFDSAKERLDKEAVDAKLKQETIDISLPGRQM
                     YVGAKHLISQVIEEIEDLFVGMGYTVEEGPEIESDKYNFEMLNFPKMHPARDMQDSFY
                     ISEDNLLRTHTSPVQVRTMLKAGGNKPIKVICPGKTYRKDDDDMTHSHQFAQIEALVV
                     DENTSLADLKGTLLVLARKMFGEEREIRLRPSFFPFTEPSVEVDVSCHKCGGAGCSMC
                     KGTGWIEILGAGMVNNNVLEACGYDSSRYQGFAFGMGVERVAILKYGIDDIRAFYTND
                     LRFNRQFR"
     gene            complement(10355..11059)
                     /locus_tag="PLP02_00050"
     CDS             complement(10355..11059)
                     /locus_tag="PLP02_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPF41889.1"
                     /translation="MPLLRSSNPVIRTVRANTYVSDRPVTYSNVAMKTVFLIGILGVS
                     AYLTILYMAQIGIGILIGAILLGFISVIIGTRSVTLSPIFSVIYAICEGVFLGYISAI
                     YAYLAEGIIPTALMTTGIVFVIMLLFYSTGIIKVTQKFASVMVVALISVIIMAFLSII
                     LPFTMTSTIYYLVCGVSALLSALFLLLDFESIKNCVDQGTDAKMGWILSLGLLVTVVW
                     IYVEILRLLLIFGRRN"
     gene            complement(11134..15606)
                     /locus_tag="PLP02_00055"
     CDS             complement(11134..15606)
                     /locus_tag="PLP02_00055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HPF41890.1"
                     /translation="MNDGMKKLLDRLGIHDDIILEYGTIEKMEVDTVQNSWFFSLFFD
                     RPVPIVRYRNFIDHLKKLPQEVDSLKSVEYQTVFGMTSDQDLLDYYDFVIDVLTEEDK
                     RILPLKDYPTDIVDGMIKIMVPQGAKSASMFRKDIEAELEHNGFATTVAISIDQNCKT
                     ISEEIEKANRSFVISSQTSANSSTVVYRELYDNRPVDTDFMSFEKVPHDEIGLEEYKA
                     KNGAKANFNLAGVVVSIEVREMRSNSQANIIITDGTDSLLVKKRLSSSDEKDFCQTLE
                     EGMGLRVSGYASFDTYYNEVFVNAISMAKSDRVLPKTGRMDDCAEKRVELHVHTKMSS
                     LDGVNSIDEYAERAKAWGMSALAVTDHGSVQAYPDFAKLTHDKNLKPLYGLELVFVND
                     ETVAIAKGESAKMLGDATYVVFDIETTGLSVLYDSIIEISGVKIKSGTNIGEFHSFVN
                     PNKPISSFTQNLTGITNQMVKDAPGVEKVLRDFYEFTKDSVLVAHNADFDLGHIYHGY
                     RENGIFAGIQPSIDTLTLAKVVYPDRGRYSLDSLCRFLKVTLDEHHRAASDAKATAEI
                     FLHMLKKIRKDGINRFQDINLIIDHKEVYKYPYPKHINLLVKKQEGLKNLYKILSMAL
                     TDFYDRDGKVVKTVLDKYRRGLLVSSGCRNSDFFEIAMVKTREELKEAASYYDFLEVQ
                     PPSSFGYLVDQIPEWQTMIQDVIRRIVEVGKELDIPVCATGDVHHLDPEDLKSREILI
                     NSPMVGGGHHYLYNEKTKPSQHLMTTSEMLDEFRFLGDDVACEIVITNPNMIADSIDW
                     IQIFPNELFAPSDEFLAEQGVPSIKSKVETMVRERAENMYGSPLPSLVKTRLDKELKS
                     IIDNQFSTIYYISHLLVKKSLDDGYLVGSRGSVGSSFVAMLMDITEVNPLPPHYRCPK
                     CHFSAFKKNDEQEREFGSTDFETKINRLFDRAECGWDLPDQFCPVCGTKLIKDGHDIP
                     FETFLGFKGDKIPDIDLNFSGDYQSKVHEYIRKLFGYNYAYRAGTIGTCGSKTAFAMV
                     RDYVEKQNELREKAGEPALIVRRAEMERLAKKIEGSKRTSGQHPGGIVVVPNNKEIYD
                     VTPIQYPGDSTDTTWKTTHFDYHSFESNLFKLDVLGHDDPTMIKYLMDLVKKDPLDFP
                     FSDPKDIPVDDTEVYRLLAGTEAIGLRPEDINSDVASFGIPEFGTNFVRGMLRDSRPK
                     TFAELVKISGLSHGTDVWNSNVQDLISGKIKEFGRVDFKKVFGCRDDIMTDLIDYGMQ
                     PTQAFEIMEFVRKGKTHVNPEKWGYYTDLMRQSDVPEWFIWSCNKIKYLFPKAHATAY
                     VLMAMRIAWFKKYRPIYFYAAYFSKRAPFFDVDAFYQGEEGIKRRMASITNDSSDRDK
                     NLETVLEVALEMVMRGFRFNPIDINRSQATEFVIDEDQKSLLLPLIVVDSLGLAAANS
                     VVEAREKKPFISKQDIRTRTKLSKTIFDRLEDLGVFEGMIEEDQISLFDLDMGK"
     gene            complement(15703..15915)
                     /gene="rpoZ"
                     /locus_tag="PLP02_00060"
     CDS             complement(15703..15915)
                     /gene="rpoZ"
                     /locus_tag="PLP02_00060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPF41891.1"
                     /translation="MSNRNKEGLRYPSIDDLLEVVDSKYKLAYIAAKRAKIIKEDDYS
                     SVENKCVKPVGQALEEILAGKVKAEF"
     gene            complement(15905..16504)
                     /gene="gmk"
                     /locus_tag="PLP02_00065"
     CDS             complement(15905..16504)
                     /gene="gmk"
                     /locus_tag="PLP02_00065"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPF41892.1"
                     /translation="MSGPSGVGKGTIRKALFQIPDNNFCYSVSMTTRKPREGERDGID
                     YFFVSREEFEQHIKNGEMLEYAEFVGEYYGTPRSFIEKKLSEGKEVIMEIEVQGALQV
                     REKMPEAVFIFIVPPSKQALYDRLIKRGTEANDVIQKRIVKAEREFILAYKYDYIVVN
                     DDVENAADRIYAIIRAEHAKTERSIYKYNKLMEGKINVQ"
     gene            16653..18326
                     /locus_tag="PLP02_00070"
     CDS             16653..18326
                     /locus_tag="PLP02_00070"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPF41893.1"
                     /translation="MSMDGKFIERLCIELDNNLAGGRIQKIYQLSKTDFLFIVRTPGE
                     TKQLYVSVSTSLTRIHLSAMRYDRPENPGGFCMLLRKYLEGGIITSIKSLEGDRIIKI
                     LVENHDELGDIEDYGMYMEILGRYANIVITEKNDIIIDAFKHISPFDEVGRTIEKGAI
                     YLPPFDGKTKTSDKEGVTAIFSYAGQLNRRYLVDNIRGISPVFAEYLLREMSKPGVSG
                     LTLLDDVQKLPINPVMMEKSGKREYYYFDIFDIGQKTFFPSLSLLLDAYFNDLGREER
                     NRQMSKYLIQFVKRETDKLANKLEKLSQDLAKADEADIYRIKGDLILQCQNEIAKASS
                     NHECFSYELDRNINIELDPLSSAVKNANQYFKKYRKLKNSVAYIEEQITIAKEELEYF
                     RQLDDQIETASLIDLEEIRQELATRGMLRQKTGKTRKKTPNYETYTDNEGMEILVGKN
                     NIQNDYITNRVARPNEWWFHVKNSHGAHVIARSESELKEPQIRTAANLAALFSEARNS
                     SSVPVDYTRVKHLKKVPGTIGSFVTYTNQKTIFIDPDNAVIEHLKSKKK"
     gene            complement(18341..20245)
                     /gene="thrS"
                     /locus_tag="PLP02_00075"
     CDS             complement(18341..20245)
                     /gene="thrS"
                     /locus_tag="PLP02_00075"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPF41894.1"
                     /translation="MIKIKLPDKKELEFPAKVNGFEIAEAISPSLKKQSIAIKVDGEQ
                     IDLARSIEKDASIELITTANPLALDILRHSAAHLLAEAVRQIYPEAHFGVGPAIEEGF
                     YYDIDLGDVVLSESDLPAIEEKMKKLASLDQKMVRREVDKAGALKLFAKDPYKVELIN
                     DLPEEEQITVYTQDLFTDLCRGPHVPSTKWLKNVKLLSLAGAYWRGDSKNKQLTRIYG
                     TAFFTDKELQDHLELLEERKKRDHRKLGRELDLFMISEYGPGFPFWLPNGMIVRRELE
                     DFWYKVHLREGYKLIQTPIMLSKELWEISGHWANYRENMYVSKIDEQEFVIKPMNCPG
                     GMLVYKRDIHSYKDFPLKVGELGLVHRHEASGALAGLFRVRNFTQDDAHIFMTEDQIV
                     HEIQELVRLYDYMYKIFKLDYSIELSTRPEDKYIGEIATWDRAEKALADALDSKGIPY
                     KVNPGDGAFYGPKLDFKLRDSMNRIWQCGTIQLDMNLPERFDLTYIDQNGEKKRPVML
                     HRALFGSIERFIGILIEHYAGAFPTWLAPVQVKIIPVNLNFHLEKANELAVMMQNAGI
                     RVETDLRDEKLGYKIREAQTKKIPYQLVIGDKEVSSDEVTYRKYGSQDQVTIGVDEFI
                     SLIKEDIEKK"
     gene            complement(20517..21419)
                     /gene="dnaI"
                     /locus_tag="PLP02_00080"
     CDS             complement(20517..21419)
                     /gene="dnaI"
                     /locus_tag="PLP02_00080"
                     /inference="COORDINATES: protein motif:HMM:NF006505.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HPF41895.1"
                     /translation="MKKINQYLGAYSQDVQPDETIEQLLEDPIIRAFVLKHDLKHETI
                     VGGINELLSYRDMKNICNQCQGIYECKLSTIGMTPILRYYANEITLEYTKCRFNNFDE
                     SKNKIDMMYLPKKVLNADITDFDFIGSERKEVHNYIMKFLNEYSPENPMKGLYLTGAF
                     GTGKTYILAVIANELAKKGYRSIFAYYPDLVRELKSAISNGDLEEKIENLKKIPVLFL
                     DDIGGENQTSFVRDEILGPILQHRVLDQLPTFFSSNMKMKVLMAAMQIQENDIEKTKA
                     FRIFERIRELAVEFELSEKPSART"
     gene            complement(21436..22659)
                     /locus_tag="PLP02_00085"
     CDS             complement(21436..22659)
                     /locus_tag="PLP02_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HPF41896.1"
                     /translation="MDGIRRSDSFTVFSKSHLCATDVQIISLLYQPIIGTGAFALYFT
                     LLGLVDHQTLVSSDYLQGDLESLLDTRLDIIENDRFRLEAIGLMETYYSDDHFMYEIK
                     PPLSAESFINDGILGQYLIASITKDRFKKILNSFKLKPPALKKYTRMTKAFDEVFRPI
                     GVTESLPESDLLGNNKGKSIHTNHTTFDWRFFIESLTDDTDWENKIQNQIKEKILNLH
                     YVYGLDELTMRSVLLKSIDENEQINVNKMAVNAREEYKQMASSIPEGAKEIEKPEERN
                     LPSDPVEYFTVVSPRQLLAEMSGGKFSAADLRIVERILEETGIDRGVLNVLLAYVAKI
                     KDGSLPGYEYFEKVALDWKKNQVTDVKTAIAYVQHISSRYQQAKESIGNRKQRTKPAD
                     DIKIDWLDEYIQSIK"
     gene            complement(22671..23027)
                     /locus_tag="PLP02_00090"
     CDS             complement(22671..23027)
                     /locus_tag="PLP02_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41897.1"
                     /translation="MSFFSSTCETRELHIDSGLRTRYYRNSFKQCLAVIEEYSGDAGT
                     EIVNVNQAHGEIHMVGSGYDSIITITQLTPLETGIDIKINFFTSMGFNRPKKKVMEMY
                     AYFDHKLNFKGVSLHP"
     gene            complement(23038..23634)
                     /gene="coaE"
                     /locus_tag="PLP02_00095"
     CDS             complement(23038..23634)
                     /gene="coaE"
                     /locus_tag="PLP02_00095"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPF41898.1"
                     /translation="MEVIGLTGGIASGKSLISGWFKEEGIPIIDADFVYKRLTKPHEV
                     LYNEIVGNMPDVSLCPDESIDWQKLAHKIYSDDSVRQKLNALSHPVVIEEILKELDAY
                     DEKGEPLVVVSAPLLYESGFERVCDVVIVVDALESVRIERLMERDHIDKDYALSKIGS
                     QMSSENRKGKADYVIDNSGSIDKTKRDFQAILQSIMRK"
     gene            complement(23653..24468)
                     /gene="mutM"
                     /locus_tag="PLP02_00100"
                     /pseudo
     CDS             complement(23653..24468)
                     /gene="mutM"
                     /locus_tag="PLP02_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
     gene            complement(24470..27091)
                     /gene="polA"
                     /locus_tag="PLP02_00105"
     CDS             complement(24470..27091)
                     /gene="polA"
                     /locus_tag="PLP02_00105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPF41899.1"
                     /translation="MKKLLLIDGNNIMFRSYYATAATGNLMQNQDGVYTNGIYGFVRA
                     FQQIFKMNFTHVMVALDAKGKTFRHDLFPEYKGTRKETPSELVMQFPLMREFLSAAGI
                     PYFEKEKYEADDIIGFSAKHFAPEFDQILLISNDRDLMQLLDEKVSQLISKKGFSDYD
                     VVTSSVMEAEMGIRPDQMTDYKGLVGDPSDNIPGVPGIGPKTAAKLLDEYKTLENVLA
                     NVGSLKGKTKEQIENNAAQAEFSKRLATIVTDFANDISIDKLEYHGPNEEMLQRFYQK
                     MGFHSFLRKLDTAKQKEDVSGFKVIGRPEEIASILTSPVALHLEIFGSNYHIAQKLGF
                     ALVGAKGSYYIPYSLLPLSPDLQNWLMDSHKEKFVYDLKQTKVALLWDGYDLEGVSFD
                     LMLSAYLLNPNMTKEGFREVVSNFDYLDVAYDEEVYGKGASYALPEDNVIAKHSIDKA
                     AAIFALHEKMLIMNKECGQEELLSNIEIPLASILAGMEKEGIAVDEKKLDDFGFQLKQ
                     RIDELETSIYELAGENFNINSPKQLGAILFEKLGLPYRKKNKTGYSTDISVLKELNDF
                     HPIIDRIISYRTLTKLYSTYYEGLKSALRLKEDGRIHTIYKQALTQTGRLSSVEPNLQ
                     NIPVKTDEGKELRKVFIADPGTKLLSCDYSQIELRVLAEMADVEGLKEAFREKEDVHS
                     HTAKLIFKNSEVSSGERRQAKAVNFGIIYGKTAWGLSEDLKISPKQAEKFIADYFDSF
                     PEIKGFMEQTIEGAVKNGYVETLFHRRRYIPEIASNNYQTREFGKRMAMNAPIQGSAA
                     DILKIAMVNMGKAIKERDLKTKMLLQIHDEVVFSVPEKEKEKALDLIIRLMEHSVELS
                     VPLEVESSFGNNLYEVK"
     gene            complement(27172..27924)
                     /locus_tag="PLP02_00110"
     CDS             complement(27172..27924)
                     /locus_tag="PLP02_00110"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPF41900.1"
                     /translation="MLVLIDIGNSTIAIGLSTDGKTIVKKFRISTGKSRSSDEYAIIL
                     DELIENADQAIVASVVPELNEVFRDYFSSRFGFEPIFLASGVKTGIRIFTDTPKEVGA
                     DIIADCVAASQIYSHTCLIIDMGTATTMTYIENSGIKGVIIAPGLATSLNALISKASL
                     LPHINLEKPSKLLGTNSPDSIKSGLIYGHASMVDGLVARVRKQTGNPDLAVVLTGGNA
                     KIIYPECQAKMTLDEDLILKGLLLVAGMNATK"
     gene            complement(27932..28579)
                     /locus_tag="PLP02_00115"
     CDS             complement(27932..28579)
                     /locus_tag="PLP02_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPF41901.1"
                     /translation="MTNEKSNKSNREKTRELTMMAMFIAIIALLGLVPSGWGASTIGF
                     IKIAPDLEATIIHIPVLIGAALFGRKMGIWLGTAFGIVSMIAAFIYSSPFFIYPWVSV
                     LPRIIFGLIIFDVVKFFQRFIKNRHLAYGVSFLILTLIHTILVLSMFWTSFALVNHFT
                     LKEAFIPYLEALVLWGIPISAPAEAILAGLVGASVTTRLKESLVRNKSLQTEEKE"
     gene            complement(28640..29704)
                     /locus_tag="PLP02_00120"
     CDS             complement(28640..29704)
                     /locus_tag="PLP02_00120"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPF41902.1"
                     /translation="MKQFYKELVNLPGISGNERFIRNYLKEKYQDVAENIYQDKLGSI
                     FGSVNETKDGPTVMIAGHMDEVGAMVASITDKGFIKMIPIGSINPPIMLSQNMNIIMD
                     DGSSVPGVVGAKPPHLLRDDKSKQVTSFDDFLLDIGADSKAHAEEMGIKVGQQIVFEN
                     IYRETFDKKKIISKAWDDRFGSAMALDIMSSVEKENIPCRLYCGANVQEEVGLRGAKT
                     SSYMIKPDFFIAVDCSPCSDTFEETETGGKLGGGFMIRFYDPRCIMHRGVRMFVEETA
                     KENNIPFQYYKSLGGTDAAEVQLSRDGVLVCTIGMPSRYIHSSASMIHKDDYEAVKKV
                     IMAMIKKLDHGMIEQIKKNV"
     gene            complement(29701..30351)
                     /gene="trmB"
                     /locus_tag="PLP02_00125"
     CDS             complement(29701..30351)
                     /gene="trmB"
                     /locus_tag="PLP02_00125"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPF41903.1"
                     /translation="MRLRKVKHAGETIRNNPKYVIAYPESKKGLWNKTFGNNNPISLE
                     IGCGKGLFLMEMARKNPDVSLIGIEKFDSVLVRALEKLIIDPLPNLLFVLADAADVDL
                     FFDSDEIKHIYINFPDPWPKTRKAKYRLTNEAFLPKYRQILSENGKVYLRTDNFPMFE
                     YSMMSINRSDDFSITDIALGKGIESDGYVETEFEIKYRKMGLPIFYLEASVERSRK"
     gene            complement(30351..31709)
                     /gene="pepV"
                     /locus_tag="PLP02_00130"
     CDS             complement(30351..31709)
                     /gene="pepV"
                     /locus_tag="PLP02_00130"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HPF41904.1"
                     /translation="MDWLKQAKKYEKEFLDVIVPLLQIPTVLEKYDPEDKEYPFGEPI
                     RKALDFVLAKGKEYGFKTKDIDHYAGYIEQGEGEEQIGILGHLDVVPAGGKWTYPPFS
                     ATVKDGKIYARGSMDDKGPSVAALIAMKMIDDANIPLKKRIRLIFGCDEESGMRGVKR
                     YIEKETMPDIGFAPDADFPLIYGEKGIFSFDILGYGDDDLLESMRAGERYNIVPDECV
                     AILKKDLSTEFTAFLQLKGYQGETDGKKYVIYGQSSHAAWPQHGVNAVFLMTEFLREH
                     TNHPLIRFIDNCLLFDHTGEKLGIDCYDELMKELTINTALVRMENNNFYVGCNIRYPR
                     NYDFEAGVVKITEKATKYGLKFETESNSPSHFVSPDDPFVQTLYKAYQKYTNDFVSPM
                     MTIGGGTYARVLKKAVAFGAAFPGDPDLAHQPDEYLEIDKLLVTAAIYAEAIEKLAGF
                     GE"
     gene            31917..34052
                     /locus_tag="PLP02_00135"
     CDS             31917..34052
                     /locus_tag="PLP02_00135"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPF41905.1"
                     /translation="MIRKIIMKNLTCSNCIAKVERRTARLPYVNSASFNYANQTLLVD
                     FKNDYDQDVALKEIKGIVDVLEDNIDTYYYEQKPMEKKSNFIREYILVLIGMLIILLT
                     FSFYHIPEYVNKIFGTNLHEYLGNEAELRWLRITLYWVGYVLLISRLLYSQVKSIRNF
                     NFFDENTLMIIATLAAMLIGKHEESIGVVILYSIGEYLQARAVRRSKSEIASLINLKI
                     EYANLLIGDSIVIKDPSQIKVGDTIVIKNGERVPVDGIIVMGSTSLNTSELTGEAKLR
                     SVDVGSKILSGNINVGNVIHVRATKEYKDSTLAKIIDLIENSTTNKSKTELFITKFAK
                     IYTPIIVGMAGLLILFGAIFDNANLMTEGGYVYRAAIFLVISCPCSLVLSVPLSYYAG
                     IGTAAKNGILFKGSAFLQKITDVQTIALDKTGTLTYGDFFVQEATSDDVLQKIASIEQ
                     YSTHPIALSIVKYNKLPLSEVQSVEEVPGYGMKGILDGKVILAGSKKFLTNNNISVTD
                     KVMSVGSLTFLAIDGKYLGYVIVKDELKESSVDTVRKFTKHFDTVMLTGDNEESAGEI
                     ASKLGGIEYYADLLPDQKLDKFNEIRTNSVSMYVGDGINDAPLLKNADIGVSMGSASD
                     LAIEVSDVIIIDNDIRLLEKAIKIGRKTRTITIENIVFSLVVKIAFLILSAVGYMWMW
                     LSLFADVGVALLCVINAIRIIYMKRYFED"
     gene            complement(34083..35321)
                     /gene="tyrS"
                     /locus_tag="PLP02_00140"
     CDS             complement(34083..35321)
                     /gene="tyrS"
                     /locus_tag="PLP02_00140"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPF41906.1"
                     /translation="MSLFSDLKWRDLAYDQTDPNLEKILDEKKLTFYIGIDPTADSLH
                     IGHLLACLVAKRLENHGHHPIIVLGGGTGLIGDPSFKNQERQLLSIQTSLDNAEGIRK
                     QIIRLLPDAQVVNNYDWISSLNTIEFLRDIGKNFNISYMINKESVRSRIENGLSFTEF
                     AYQIIQAWDFEHLYASYGCKLQIGGQDQWGNITSGLELIRKLHGADSEAYGFTFPLVT
                     KSDGTKFGKTESGTLWLDKEKTSPYEFYQFWINTADDDAINYLKKYTFLSKDEIEKIE
                     LSVKMEPETRMAQKELARQITTLVHGENECLRAERITDALFTGKIHTLDAEEIGIGFK
                     NLIKYETDKAETIDDVLVNCQLASSKRQARELVTSGAITINGEKVTDTYYMADSNQAI
                     GKMYTVLRKGKKTYAIVKHL"
     misc_binding    complement(35379..35572)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35556..36575)
                     /locus_tag="PLP02_00145"
     CDS             complement(35556..36575)
                     /locus_tag="PLP02_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF41907.1"
                     /translation="MNKATIYNVAYAANVSLATVSRTLNNPQKVKPETRERVLRVIKE
                     LGYKPNAFAKGLASKKSTTVGVIVPDMSRASVAEMLNGIAVEARKYNYSLLLYVLKDD
                     KIEEPEALQEVVASQVDGILYLNDEINEEQYSLLNSIQDDYKVPIVLANTLYPYDNKL
                     VTVCIDYFKASYELTMRLVNEGRKNIYLLTTARKYMVNDLKEEGYLKAIKDAGLQPKV
                     VRTSGDITINTVHFAELFKNPATLIDGAIAVRDSIAVSFVNSIIERKKRVPEDISVYG
                     FQNTKYASLTRPKLSCVDIPIIDIGAKAMALLKKEIDGEPVEVKFNVLPHSIMERQTT
                     KYNEE"
     gene            complement(36726..37073)
                     /gene="rplS"
                     /locus_tag="PLP02_00150"
     CDS             complement(36726..37073)
                     /gene="rplS"
                     /locus_tag="PLP02_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPF41908.1"
                     /translation="MSQQIINMVTSEFIRDDIPSFRPGDTLKVSVKIKEGNRERIQVF
                     EGLCIKKQGGGVSETFTVRKISYGIGVERTFPLHSPVIDKIEVVRQGKVRRAHLGYIR
                     GLSAKASRIKEKR"
     gene            complement(37189..37875)
                     /gene="trmD"
                     /locus_tag="PLP02_00155"
     CDS             complement(37189..37875)
                     /gene="trmD"
                     /locus_tag="PLP02_00155"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPF41909.1"
                     /translation="MKISVLTLFPDIFGCFLSESIIGRAQKAGIVDIELINFRAFSTN
                     KHMQVDDSPYGGGAGMVLSVEPIYRALVNIDGYEKALKILLTPQGETYNQTKAIELSS
                     YEHLILICGHYEGFDERIRDLVDMEISVGDYVLTGGEVPAMILIDSIVRLLPGALGKE
                     ESYRDDSFFTGLLEYPQYTRPYDFMGQFVPEVLVSGNHAEIEKWRREQALKRTLERRP
                     DLVKKPNKKD"
     gene            complement(37872..38375)
                     /gene="rimM"
                     /locus_tag="PLP02_00160"
     CDS             complement(37872..38375)
                     /gene="rimM"
                     /locus_tag="PLP02_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPF41910.1"
                     /translation="MDLIKIGKIVNTRGLKGELKVVVMTDFAENRFAFGKTLLIRQGK
                     EDFSVKVESNRLFKNMLLLKLEGYDDINDVESFKGSDIFVEDEDDLALGENEFHADDI
                     IGLLVMQNGTKKGNVKGIRELPQGDYLEITGSEGKISLVPFRDEFVIKVDLNAKIIEI
                     VDMEGLL"
     gene            complement(38407..39615)
                     /locus_tag="PLP02_00165"
     CDS             complement(38407..39615)
                     /locus_tag="PLP02_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPF41911.1"
                     /translation="MDLTILFYAGIMIATGLLFGKLAKIAHLPNVTGYLIGGLLIGPS
                     LLNIIKESYTESLDLVSMVALGFIAFTIGDEFKVSYFKKVGAKPIIIAIMEASFGVLF
                     VFAGLILYFAITKQLDITSIRFSLILSAVAAATDPAATLMVVKQYKAKGSLTDTILGV
                     VALDDSVAVMLFGICVAVANAIGPSTAGASLIIQILNPLKEIFFSLGIGAAMGFLLTY
                     ACLWFTGRGNRISLVVTMIFVTIYLADLVNGSTILACMALGAIFANFSKKYDEVNNLV
                     YFITPPIFIMFFVLSGAELKLSVLLVVGVIGIVYTLMRVFGKFFGAWFGARISKAEPK
                     IANYLGFALLPQAGVAIGLSLVATQILDADMGSQIRAIVLAATLVYELSGPVVTKTIL
                     RKTGEITVRG"
     gene            complement(39629..39964)
                     /locus_tag="PLP02_00170"
     CDS             complement(39629..39964)
                     /locus_tag="PLP02_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41912.1"
                     /translation="MKLVFFVLNDVTKLDEFLHSLKENGIHGATIISSSGMARKLIEN
                     DDMKFIGSLKALFDNPRVESEVIMMVVEDGQVETVYQVINDSIGDLSQPNTGIAFTVP
                     IENVKGYKC"
     gene            complement(40034..40849)
                     /locus_tag="PLP02_00175"
     CDS             complement(40034..40849)
                     /locus_tag="PLP02_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPF41913.1"
                     /translation="MDTKQNTMIHDFEIKELGGRNKRIRVFLPVDYDYSEERYPVLYM
                     HDGQNLVDPAPFSGYSWDVCQTAERLFNNKLARRIIIVGIDNDPKMRILEYSDSLSPW
                     MRKRLDKEMPEEKNEPEGDIYAGFVTKIVKPFVDSHYRTLPDRLNTGVAGSSCGGNIS
                     LYMLCRYPDVYGKAGIFSPAIHVVGKSLFRLLEKTDLPPDTCIYHDMGAKETPFAAFT
                     VLRDTKKLQKLLKNKKIHEDRLIHVIDKKATHTELFWQFRFPTFLAAFFPFSK"
     gene            complement(40856..41578)
                     /locus_tag="PLP02_00180"
     CDS             complement(40856..41578)
                     /locus_tag="PLP02_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HPF41914.1"
                     /translation="MIWFLLGVAVVVAGLYIYSEYHNNPLIALFSKTLASFCFIFLFA
                     VVIYEKMVWVESPHYLGADFIVFQPAAILFTLALIAGLIGDILLALRPLRPREENESI
                     ILSGTIAFASGHVFYYFALLHLSDWSVYPFVFTAIMTTAIFFVAIKVLKFNFGKVLFP
                     TIAYSFMIFLMIGQSFANAVQNNFNTFSVIIMIGAILFGISDLILSEIYFKKDHPKSF
                     VALNLMTYYSAQILLALAFLFA"
     gene            41720..42844
                     /locus_tag="PLP02_00185"
     CDS             41720..42844
                     /locus_tag="PLP02_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPF41915.1"
                     /translation="MKKVLKIFLILVCAILVFGIGFILTLQIFEYRPDDITILAIDNE
                     PENSVTNYVGIDETVSIMTFNTGYASLSATEDFVMDGGVKGRMDSIEEVEVNIAGIKS
                     IITAAEADIYLLQEVDTDSDRSYNTMQYDEYSTLLSYSGSLGYNYRCIFVPFPFKMGQ
                     MMGSVDSGIATFSRFYTSDATRYQLPGSFSWPVSLANLKRCVVVSHLPISGSDKYLTI
                     VNVHLSAYDDGTMRIQEMEALQNIVEAEYEAGNYVLVGGDFNQTFPDAVTITENIDGT
                     ETYDYLYPLKEAGLWEAYPMSDTWFTDNGFTFVNDISTPTCRLLNQPYDSENHDNNQY
                     YSIDGFIVSANITVESIQVIDQDFMYSDHNPVLIEISFIH"
     gene            complement(42897..43271)
                     /locus_tag="PLP02_00190"
     CDS             complement(42897..43271)
                     /locus_tag="PLP02_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPF41916.1"
                     /translation="MKVALAVTDNMVTEHFGHCDYFVVYEIEDGKAKGSEIIKNPPHQ
                     KGFLPNFLHKNGVNCVIAGNMGEMAKKIMDGFGIQVIMGINGEVSDVIAKYLAGELVS
                     SDIICRQHEHHHGEDGNHGNHE"
     gene            complement(43243..43644)
                     /locus_tag="PLP02_00195"
     CDS             complement(43243..43644)
                     /locus_tag="PLP02_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPF41917.1"
                     /translation="MPRPHKPRCICKMPEFQKYGPLDRNSDKSETIVLNLDEYETIRL
                     LDQEGLDQEEAAKLMNVARTTVQRIYDEARKKIANALVSGQNLVIEGGHYVFCEKDCN
                     SCVRPRHSNNSSCRHMNGGGKSNESGFGGNR"
     gene            complement(43705..45369)
                     /locus_tag="PLP02_00200"
     CDS             complement(43705..45369)
                     /locus_tag="PLP02_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HPF41918.1"
                     /translation="MKTDSLTLEQKILRRNKWNYSVGGIGRDMIYQLIATFLMTYVQY
                     SGLGLSTAQFSAIGIILIFGRVWDAVNDPIMGSIVENTHTRWGKYRPWVLIGGILTSI
                     MILIFFIVRPHFWNNWGYVFFFAMMYLLWEMAFTLNDIPYWSILPALSRTKKQRDQIA
                     TMVVVFAAVGAIMANAIIPMVTVGNMVTGYQTIAITFVIIFLACTCLTVFGVKEPQDE
                     MQASPEKVTIKKMFQVIKNNDQLLWASLALMLYSVGSGLLTALGYNFFYIELGYNGST
                     ITIFVGVLFIVNVLVQSTYPLMAKKFSRKQLLGTGFIVLTAGYLMFLLMGYLPIFPVS
                     LISMCIFGALVFGGQAIFYMVIILNMTNSIEYNEYRSGQRNEAVVFSLRPFVAKFSSA
                     LQQGIYILVLVMSGIYVLSQNVSTLEEVKNSFDNIVDVQEQETFISNVAARQTILDEY
                     DLTAAEESNIYDALQEVTFEQNEETGMYSMVINTAADSVLKDQTTGTMRLLLRISITI
                     IPIILITGSFYILRKKYFIDEKYYEMINKEIEERKEKEQTPQENPA"
     gene            complement(45535..45723)
                     /locus_tag="PLP02_00205"
     CDS             complement(45535..45723)
                     /locus_tag="PLP02_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41919.1"
                     /translation="MRYRETKEEKERKKAQALKNLQETPPEKNDVKAMIIAAFLVFFP
                     VLILILAVVFGVLLLIFT"
     gene            complement(45725..47287)
                     /locus_tag="PLP02_00210"
     CDS             complement(45725..47287)
                     /locus_tag="PLP02_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF41920.1"
                     /translation="MGNTAKNREPLIQVKDLKKYFPLKKNTFMNRNQIYIRAVEDINL
                     TIYKGETFGLVGESGCGKSTLGRTILQLYEPTSGSVTYYGDLVGETSLKYVETMVDKL
                     PYNQQKAKHYFYLSKEVDSKIPAFDAELLQIDKQLKSPLQEQEKKKLESKQADLAHKI
                     ALLRQKSFDYKKQSSRYLRESSHLVGSLILCQNIEEIKELLKKTVEFAKHYRDVIDGL
                     NGKYASYFTPEIIATFEKVNSFHGKNLVAINDYAMKPEFQAKLDWYKESGIILNKLSY
                     EDMRMLRRKLQVIFQDPYSSLDSRMTIGQIIGEAVAEHGLYKRGSKELEDYVLQTMEK
                     CGVAHYMLHRYPHQFSGGQRQRVGIARALALDPEFIVCDEAVSALDVSIQSQIINLLA
                     ELKEKENLTYLFISHDLSVIKHISDRIGVMYLGTMVELGPSDEIYQNPLHPYTKALMS
                     AIPTTDEVQPERIILEGDIPSNIFPPSGCKFRTRCPLATEKCAQLVPEYKEVLPGHFV
                     ACHYYEKTKDMK"
     gene            complement(47265..48272)
                     /locus_tag="PLP02_00215"
     CDS             complement(47265..48272)
                     /locus_tag="PLP02_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF41921.1"
                     /translation="MSLLEINNLHTYFTTKRGLIKAVDGVSLKVDEGKTLGIVGESGS
                     GKSVTVMSILKLFESNQKIHEGEIWFEGEKISEYGEDEIRKIRGNKISVIFQEPMTSL
                     NPVMTVARQIGEVLMLHQHMNKEQARERTIQLLGSVGISNPEKIADTYSFQLSGGMSQ
                     RVMIAMALACRPKLLIADEPTTALDVTIQAQILRLMNDLKRELGTSIIFVTHDLGVIN
                     EMADDVVVMYCGQVVENAPAKVIFGMTSKYMHPYTEGLMRSIPRLSDDKNRKLDVIPG
                     SVPHPLDLPVGCKFSPRCKYATEKCHNQEPETIEVEPGHGVRCFYPESEVRRSGKHSE
                     E"
     gene            complement(48282..50186)
                     /locus_tag="PLP02_00220"
     CDS             complement(48282..50186)
                     /locus_tag="PLP02_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF41922.1"
                     /translation="MKTLKKIWKSICEMFKRLFVPEKNIKSILDEEALQSPGRVIAKK
                     FFHNRLGIIGLVGFILMLIFTFGLAIFFPLDLYRSSAFHTNLPPNYSYLSYPKELEKE
                     GVVSIDSGVAFSIGLSEEGNVFVWGSNIYDIKVIPEAVQNANIVQISAGTRHLMCIDD
                     QGNVYLWGYNHMQQAQIDDRYAEALLADPIVSLEGGYDRTIAITESGAVYVWGVGTNI
                     VGQPMRRSPYIYTDTLGNPIKAVQAVANNNNLIILLEDGTIRIVGTASEVKTSVPVEL
                     TDGSVFVTKVALTEENCFAIDSDGNLYDWGSTSRQLIDVVNLSGEIVQYVPDGARTNV
                     KDIQAGYENIVVLTNDGYVYTWGNNDLNQLNLPDDIDGNVSEIFVNAYQNYLVTDDGN
                     IETWGLKGFLLGTDESGRDFVLQLIHGGKITMTVGAVAVIISVVIGTVIGMVSGFFGG
                     WVDNILMRFTEIVSSFPFLPFAITLSYLLLNATVDTSEITRILMIMIVLGVLSWTGLA
                     RLIRGQILSERERDYVLASRALGIKKKHIIWRHIFPAVNSLVLVNLTLGYAGSLLTEA
                     GLSFLGFGVQKPNPSWGNILTVAQDREVFDTYWWRWVLPGLCIIIAALSINLVGDALR
                     DAMDPKNNER"
     gene            complement(50201..51175)
                     /locus_tag="PLP02_00225"
     CDS             complement(50201..51175)
                     /locus_tag="PLP02_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF41923.1"
                     /translation="MLKFMGRRLVSLIPVVLIISVMLFVIMKLMPGDPIKYMIPPGAI
                     RDPDIYQTLYDSWYIRLGYDKPLYIQYFIWMGNMITGNFGYSTYWMTDVKDVISRPLL
                     NSVLINVFSIVIAFAISIPVGIKTAVKKDSFSDHGWQVFSLIGMSMPTFFIGLTLIYI
                     FSLRLGWFPSGGMPYESPGTPEYYVSYLKYLFLPVVTLTIGALAGTIRYVRGSVLEAL
                     SKDYIRTARSKGLSQKVVIYTHAFRNALIPVVTIMSYSVVGLFSGSAITEQIFVWNGI
                     GTVLIKALNNKDYNVVLILDMFYALLSLLANIIMDISYALVDPRVKLE"
     gene            complement(51282..53102)
                     /locus_tag="PLP02_00230"
     CDS             complement(51282..53102)
                     /locus_tag="PLP02_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPF41924.1"
                     /translation="MLVVTLFACGTTTAGEETTTTTTERQPQQLVVGVPEMNGEFISG
                     FGNSSYDNYVRSLIYGYATYAVTPAGEIVINDTVVSNLATSTDDDGNKTYTFTIYDDL
                     YWSDGVQITAEDFVFSVLFTASADWREVGGADTTSGEGLLGYSAYNDPEGSTSEGIYF
                     EGVKLIDEYTFSLTIDAAELPYFYETAYVSYGPMPMHELAMGSCYIDSDETGSTLYYG
                     FLKIANIVDVDGYRYYPTVTAGPYKFVSFVNQVVTLELDPLFKGDYKGDNATIDSVII
                     KRVNQTLDVDLVISGEIDLVTGVIEGSKIEAAEAAATAETNYYNRNGYGLLAMTCDFG
                     PTQDEKVRQAIAYITDRQYVVDQVLGGYGSIVYSEYGLAQWMYQDSEEWVEDNLNTYD
                     FSISAANALLDQTEWKYESDGTTLWDATKATSDNAYYRYNASGDRLVIKHFGTENNTV
                     TTSLRDKFMVNMPLIGAEYTITIGTFDTLLDHYYFGFDLGEDRTYCLFNLATNFGTTY
                     DPYYSWHSDFLNTWINSQGMDNEDLDALIVAMRETEPGDNETYLGLWRQYQALWNELI
                     PNVPLYSNQYYDVYDSNLHGVETTPFFDWTQAIYTMYFGD"
     gene            53842..54843
                     /gene="argF"
                     /locus_tag="PLP02_00235"
     CDS             53842..54843
                     /gene="argF"
                     /locus_tag="PLP02_00235"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPF41925.1"
                     /translation="MSNNFKGRSLKTLLDYTQAEIYELLDIATDLKDKKHHNKDFTPL
                     KGKNVVLLFEKDSTRTRCAFEVGALDLGMGATYLGPTGSQMGKKESIADTARVLGRMY
                     DGIEYRGYSQKIVDTLSAYAGVPVWNGLTNEYHPTQVLADLMTIREKFGHLEGLTLAY
                     FGDARYNMGNSLMIGCAIMGMHFRAVAPKSFWPDQNLVKKCQEIAGRSGGSITLTESP
                     AIGANDADVVYTDVWVSMGEADTEWQKRIEVMKPYRVTKIIMDKASKKAIFMHCLPAF
                     HNTDTIIGKDIFEKFGIDCMEVTDEVFESPQSVVFDEAENRLHTIKAIMYASLAEDL"
     gene            54843..55772
                     /gene="arcC"
                     /locus_tag="PLP02_00240"
     CDS             54843..55772
                     /gene="arcC"
                     /locus_tag="PLP02_00240"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPF41926.1"
                     /translation="MKIVIALGGNALGNNPEEQIQKAASAVKPLASLIKTGHQVTIAH
                     GNGPQVGMINLAFETASLTDPKQPSMPFPECGAMSQGYIGYHLQTSLKKELSIGKMEN
                     PVVTLVTQVEVSKEDPAFSNPTKPIGSFYSKEDASELAKAKGYVMKEDANRGYRRVVP
                     SPMPIDIVEIDSIKTLVNQGCVVITVGGGGIPVIRDKEGYQGVAAVIDKDFASAKLAD
                     LLDCDMLVILTAIDKVKINFGTKNELSLDNVSVVQLKKYIKEGHFHKGSMLPKVEAAI
                     KFVEARQGRTAVIAFLDEAVEAVNGMAGTLIHN"
     gene            55789..56844
                     /gene="ord"
                     /locus_tag="PLP02_00245"
     CDS             55789..56844
                     /gene="ord"
                     /locus_tag="PLP02_00245"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HPF41927.1"
                     /translation="MRKDPVKVLIWGFGAMGSGMARMIIRKQGFDIVGICDRNPNVVG
                     KSIFPLLKETNPYDHDVVVKDDIDALLSETKPEIALLATDSFTKKAFPKLIKLIEAGC
                     DVISTAEEMAYPKANEPELAEELDKAAKKAGVTVLGTGVNPGMMMDLLAIALTGVMTD
                     VFDMKISRVNSLSPFGETVMQEQGVGLTPKEFTKKQATGEIAGHVGFRESAMMIADAI
                     GLSIKKFNQSMTPIITNIDRKSPYGFAAKGNVCGVAMKANAELENGMEIQLDHPQQIE
                     PAKGGVTTGDYIKIAGLPPINLANTPEVEGGLGTIAICVNMIPIVLNANPGLKTMIDL
                     PVPRAIVGDVRKLIETK"
     gene            56850..57155
                     /gene="ortA"
                     /locus_tag="PLP02_00250"
     CDS             56850..57155
                     /gene="ortA"
                     /locus_tag="PLP02_00250"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HPF41928.1"
                     /translation="MVEKNTWVQIHKILLKPEERTANLPDDTKKVPLEMWVKGFLLAD
                     AMIGDIVKVKTRTGRIEEGTLIKANPAYLHNFGNFVPELLKIDDMVKHAVFGSDEDE"
     gene            57148..58563
                     /gene="ortB"
                     /locus_tag="PLP02_00255"
     CDS             57148..58563
                     /gene="ortB"
                     /locus_tag="PLP02_00255"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HPF41929.1"
                     /translation="MNDMSYQALIERKNEIMKKSVGIDYSKYENGKIGFDYERLLSDS
                     GYDLEEIMKIQKASGVGNTPLKELKNMTMLARKVAPKGYGARIFVKDESLNDSGSFKA
                     RRASISCYHAKKIGYKGVIAATSGNYGAAVASQAAKYGLKCIIVQECFDSRGIGQPEI
                     IEKARACEAYGAEVVQLSVGPELFYTFLRLLEETGYFNASLYSPFSVAGIETLGYEII
                     QDSLSLTGMEPSAIVVTNAGGGNLTGTARGVLKATDRKVQMIAASVNLKGLHMASDHD
                     FNRKSFTTGHTGFGIPFVTLPDRSDVPRSAARPLRYMDRYVTVNQGEVFFITETLTAL
                     EGIERGPAGNISLAAAFALSKEFNENEVIVVQETEYTGAGKHHQAQLDFARQNGIDIY
                     FGNPSEEIPGQSIVLPSDLSLLRTDELDLGKLRDSFIHNAVKKHDSLAKSDIKFLVDE
                     TRATEEFVKNTLSNLGIGMEE"
     gene            58566..58943
                     /locus_tag="PLP02_00260"
     CDS             58566..58943
                     /locus_tag="PLP02_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HPF41930.1"
                     /translation="MDKKTIRPDDYESRSIHLKKLTDDELKNYFWTLAEKAVDPLINL
                     AQKNTSPAIERSVLLRMGFSSIEAKAIVEKTIQNQLMGKGAGHVVYRYSQIKNIGIRD
                     AGLELMKDSGWSLVSESFGVKKS"
     gene            58940..61141
                     /gene="oraE"
                     /locus_tag="PLP02_00265"
     CDS             58940..61141
                     /gene="oraE"
                     /locus_tag="PLP02_00265"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HPF41931.1"
                     /translation="MKPNDKISLDEIFKDLDKYTPRRHGWTWRKPVPGQIFDNQKYSD
                     TSASLKNYVPLPAAKYFGNIDPQPIQVITTEIASGRFEDDIRRMRMAAWHGADHIMVI
                     RTAGQSHFDGLIEGTPQGIGGVPISRKQIRAQRKALDMIEDEVGRPINYHSYVSGVAG
                     PDIAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDAAESKKIMAGADMLQIDGAHNA
                     NATAMVAWKVMPELMVQHAINSLFSVKSGMKKENIALSTVPPTAVPAPDLKVNLPYAV
                     ALRDFFEGYRFRAQMNTKYMESSTREATVTHVLNMMISKLTSADIQSTITPDEGRNVP
                     WHMFNVEACDTAKQALVGMDGLKEMVEIKKDGILPAKVRELKERAVLFFEELLAEGGY
                     FEAVQKGFFIDSGIYPERNGDGISRNLNGGVGADTVYERDKDYFAPVPSHFGYNNVSQ
                     YNLSSNDNPAVLIGGSTFENREKIQYIDELDNEDNVYIRMGETAKYRDSSMIKPEVEW
                     LGDGVVVIDLTLPCGSDYAKAAGIEIAKRMNLSDAEVIHTEILHPAEATRIQVKGTVN
                     FDIDLLTLTLPKKPDLLTDQEIRDYVSLHPLTIVAGTVGEDEHSVGLREIIDIKHGGI
                     EKYGIKCIYLGTSVPIAKLVDAAIETKADAILLSTIISHDDIHYRNMKKANDYAIEKG
                     VRDKIIMIAGGTQVVPDTAIKQGMDQGFSRGTKGQDVASYLVKRHKELIKK"
     gene            61138..62502
                     /locus_tag="PLP02_00270"
     CDS             61138..62502
                     /locus_tag="PLP02_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HPF41932.1"
                     /translation="MKIDALVAEIGSTTTVISAFDKLDCNPVFLGQGFAPTTVLEGDV
                     NLGLTNAIIDLKQKLGILELEAKETFACSSAAGGLKMSVHGLVYDMTVKAAREAALGA
                     GANLKLVSAGMLNDEDINYITNSNLNIIMIAGGVDYGETATAIANATIIAGLHLSIPV
                     IYAGNIQNQQTIANIFVKEGQSDYLFLTKNVYPRIDELETDEARKIIQSVFEKHIIKA
                     PGMEKVREMISENIIPTPGAVMDAALLMYAEIGDLVTFDIGGATTDVHSVTNGSERNE
                     KIMLGPEPFAKRTVEGDLGVYVNHDNIIKMIGKEKLASDLGISISQLESMLAGYLAIP
                     VKSHYDMVMRLAKEALKYGLERHAGKYISLYGASGKTTMAEGKDLSAIKYVIGTGGVL
                     AKLDGKNIISTVLLQASDTMMKPNPDAKILIDKDYIMAACGVLSHKHPSAATTLLKKS
                     LGIG"
     gene            62507..63568
                     /locus_tag="PLP02_00275"
     CDS             62507..63568
                     /locus_tag="PLP02_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HPF41933.1"
                     /translation="MYPQLVIDVKKLKSNTEKVLSLCHKHGISSTILVVKVFAGFLPI
                     VEILAQNEFDYIGDSRLENLERFQNISKPKCLLRIPSPSQAEKTVRYANLSLNSELET
                     IKALNEAAQRQNKIHDIILMFDLGDLREGIFFMDDYLPTVKQILTLKNIHLKGIGTNL
                     TCYGGVIPTVENLTELVNIKTKIETQFSISLDIISGGNSSSFVLLESGNVPKDINNLR
                     FGEIVYMGRETAYGNYLEGLYKDIFTLEAEIVELKQKPSYPIGVIGMNSFGEKPNIPD
                     QGTMTRAILAIGKQDVILENLTPKDKSVSIIGGSSDHLIVGLSEGSHKPGDIIAFDVN
                     YPGLLHLMNSHYIDKVIKQ"
     gene            complement(63597..64358)
                     /locus_tag="PLP02_00280"
     CDS             complement(63597..64358)
                     /locus_tag="PLP02_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPF41934.1"
                     /translation="MEIENKSIALLIDAENISPAYIEIIIDEANKYGKINYRRVYGDW
                     TTTQLNTWKQRISEFGLTPVQQYSYTSGKNASDFTLIIDAMDILYSGKVNSFCIVSSD
                     SDFTKLVTRLREDDMFVFGMGESKTPISLVNSCETFSYLDKMVVPEHPEITPCTDKKD
                     DDKTSKKKTNGKSESSIPPLSKIKAELKNIINDNMEDDGWAYWSLIAQLFQRKFPGFH
                     PRNYGSNMRPLNFFEKMEEFEVKKINTVIHIRNKA"
     gene            64435..66246
                     /locus_tag="PLP02_00285"
     CDS             64435..66246
                     /locus_tag="PLP02_00285"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPF41935.1"
                     /translation="MTRRKQMKNNELEKWLEEITAWCSPDKVVLWHGTKDEYDRLAQS
                     MVDDKKAIRLNEKLRPNCLLFRTDPSDVARVEQRTFISSVEEKDAGPTNNWIDPKILK
                     PTMWEKYHNSMKGRTMYVIPFSMGPVGSKFSKLGVELTDSAYVTLNMHIMTRTGDKIM
                     EMIDKGTEFVKCLHSIGYPLNEGNNDPLWPCAPIEEKYISHFPEDNMIWSFGSGYGGN
                     ALLGKKCFALRIASNQARKENWLAEHMLILKLTSPEGETKYITGAFPSACGKTNLAMI
                     RPTLPGWVAETVGDDIAWIRLGKDGGFYAVNPEAGFFGVATGTSWESNPNAMESIKND
                     TIFTNVALTDDGDVWWEGMTKKAPDHLIDWHGNDWTPESGGKAAHPNCRFTAPAANCP
                     SIANNWEDPEGVPVSAMLFGGRRPSTIPLVHESFDFNHGVFLGSIMGSEITAATISND
                     IGKVRRDPFAMLPFIGYNIADYVNHWLEMGKLANKDKLPRIYYVNWFRKDPEGRFLWP
                     GYGENIRVLKWIFNRRDNLADAKKTPIGYVPRPEDLDLEGLDISDENLKKLLDVDVNS
                     WTNEAASIRDYYEKLYGDTLPQELGKQLSELEMRLKA"
     gene            66363..68267
                     /locus_tag="PLP02_00290"
     CDS             66363..68267
                     /locus_tag="PLP02_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPF41936.1"
                     /translation="MSRIIREKGSLFLVFLFFFLLTEAITFRWVGFTTFSQFLYIDII
                     MALIIGSIAILFRSNVVSLVIYSFIYGIVIALFMINVTMYDIYYDLFSLQQLQLINEA
                     ATVFNFAFLSIPSIIISVILCLLFLTINILLVIYFKKHPQKVDRFHLKTVFMFVVINM
                     CAITLFASGIPSVSEYKSQSNITTFKRASLEKYGTLGYYAKEAEDIILREYNLTDDGD
                     NNETPSNIVYADQTDYFGLLEGMNVITIMIESGQPFGISEVLTPHLYDLTQEGLYFPN
                     NYSENKTNISEVAAITGNYPSINFLPGSYDYEMPFAVPNILADTYETMYFHDNVPSFY
                     SRGALLPQLGFENIYLHDDLYPGEDIWTWNGDYTLDSETIEKILPILTSSGEPFYAFW
                     STLSTHGPYNCGRNNIISFAEKNYFARIDAAEDAGLWTNVLANGEATDASKIRYYQAA
                     MMNLDDAVGRLLEELENNNMLDNTVIILYGDHNVYYHDLSTKMYADSGNEYYNMEMYH
                     TFFCIYNKTLTQAYLENTGSDSTSIEDFTSPYSIVPTLLDLLGYSYDKNIYLGDSIFS
                     GKMQVFYSHKLSGLFNNIIYSNDGIDIDYSSISVDDDYIDSFREACEELIVRLEYVNY
                     AYYSTQTNRD"
     gene            68369..69076
                     /locus_tag="PLP02_00295"
     CDS             68369..69076
                     /locus_tag="PLP02_00295"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPF41937.1"
                     /translation="MPHIYGPEHIIYLFVVIVLMGMGFFLINRHVKTEKQLALAIRLI
                     GGLLLIMIIWNRLSIAILRDGFNELLPGSYCGTSSLVLALAAIFLKKDHPVFHCVAYI
                     GLLGGLATLAYPDFIGQADSIFYPMTISGLAHHTVMVFLVVVMLTTGYLKPMFLKWHY
                     AVLGLALYLVYGLFLIKVLGYGEAMCINRPLLDDTNLYCFQLGAIYLPVHAAFLAIWN
                     LVGKKYRIGAMLAKVAS"
     gene            69208..71682
                     /locus_tag="PLP02_00300"
     CDS             69208..71682
                     /locus_tag="PLP02_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF41938.1"
                     /translation="MGKKILIVGGVAGGASTVARLRRMDEKAEIIMFERGEYISFANC
                     GLPYYIGGTIGSRDALIVQSVEDMETKFNLDIRILSEVTSIDAKNKSVTVTNHKTNDT
                     YKENYDFLVIATGAYPVKPPIPGILEAKNLFTLRNIPDTDDIKGFITNNHPHKAVVIG
                     GGFIGLEMAENLKHQGLDVTIVEMADQVMAPLDYEMAQIVHQHLAEKEVNLILKDGVK
                     SFQEAGKKVQLTSGKVLDSDITILSIGVKPDNQLAKTANLETGLRGGIIVNEHLLTSD
                     PFIYAIGDAIEVVDFVNKGKTMIPLAGPANKQARIVADNICGIDEIYPGTMGTAIAKV
                     FDLAVGTTGNNERQLKSTGVRYSIIHTHPGSHAGYYPGSSTISMKVLFDPETERILGA
                     QAIGMEGVDKRIDVIATAMMAGMKITELKNLELSYAPPFSSAKDPVNMIGFVAENILK
                     GLVETFQWYDVDELVREGEFLLDARETIEHELGTIPGSVNIPLSVLRNNLGKLPKTKT
                     IYLYCQAGIRGYIAARILMQNGFKVKNLDGGYKTYQIVFDPAHDAGSKAIDDAGTMKI
                     EEEQKPMENETNPKITLSVDACGLQCPGPIVQVYKNLETMKEGEVLEIKASDPGFKKD
                     IWKWAEKTGNTVLDLKTEDRVITALVRKGIAKASKPVENFVTSDKENTTIVVFSQDLD
                     KAIAAFIIAQGKAAMGKKVSLFFTFWGLNILRKPKKVRVKKTFIEKMFGKMMPRGVDK
                     LPISNMNMAGMGSKMIKAIMKKKNVDSLQVMMKNAMSMGVKITACAMSMDIMGIKKEE
                     LMDGIEIAGVATYLADTTEANHNLFI"
     gene            complement(71712..72128)
                     /locus_tag="PLP02_00305"
     CDS             complement(71712..72128)
                     /locus_tag="PLP02_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPF41939.1"
                     /translation="MISYRKELWFHTEARREFVNITKQVSGCIRESKIMEGLVLVNPM
                     HITASVFINDDESGLHSDFERFLEKIAPEKPYGQYAHNGYEDNADAHIKRQIFGREVV
                     CAVTNGKLDFGPWEQIFYGEFDGMRDKRVLVKIIGE"
     gene            complement(72225..72467)
                     /locus_tag="PLP02_00310"
     CDS             complement(72225..72467)
                     /locus_tag="PLP02_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPF41940.1"
                     /translation="MKIIVMGGGCANCKRLLQMVNHTVEKLGIDAEVSYVTDFAEIAK
                     SGVMRTPALFIDGKLLSAGPVPPLSEIEEMIAKANK"
     gene            complement(72485..73510)
                     /locus_tag="PLP02_00315"
     CDS             complement(72485..73510)
                     /locus_tag="PLP02_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004898196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPF41941.1"
                     /translation="MQIIKDIFAWMNDQLLKMLWLDDIVKWLFGRVFGISETSAAYSF
                     LTFFFYDVIKILILLSVLIFISSWIQSRFTPERSRAIMSKFKGLPANIIGALLGTVTP
                     FCSCSSIPIFIGFTRAGLPVGVTFSFLISSPLVDLASVVLLASIFNWKIAIAYVLVGL
                     IIAVIGGTLIGLFKMDKYVADFVKADGEVAMEEMPETVMTKKERAKYSWQQVTDIVKK
                     VWPYLLIGVGIGALIHGFIPQNWIEAVLGDNNPFSVLIGTAVGIPMYADIFGTLPIAE
                     ALVLKGVGIGTVLSFMMAVTALSLPSIIMLRRVVKPKLLWTFVGIITVGIIAMGYLFN
                     AFYIYLV"
     gene            complement(73498..73785)
                     /locus_tag="PLP02_00320"
     CDS             complement(73498..73785)
                     /locus_tag="PLP02_00320"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPF41942.1"
                     /translation="MENQMAEAFRALSDPNRLLIMKLLLEGESCGCTLINSLPISQPT
                     LSYHLRMLSASGLVTSRKEGTWNKNFVNKEKIKETIKYLEELLETKKTCKL"
     gene            complement(73914..74480)
                     /locus_tag="PLP02_00325"
     CDS             complement(73914..74480)
                     /locus_tag="PLP02_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41943.1"
                     /translation="MGLLFYHCSSVARIKDLEPNTPNYFDKPRLVSMTTLLPMALFYG
                     ISHFEYTYGYNREKKLYYMEHFPDALEKLYSGKKAFLYQCEDKDNYEKTMIPNEFVSR
                     SSVHVIGVVVIEDLYEEILKQEKLGNLIIIRHQDLNNENREWIKNAETEVILKKGLLD
                     QKSAFSEYMRMTYPDSWILAETRTKCAK"
     gene            complement(74491..75351)
                     /locus_tag="PLP02_00330"
     CDS             complement(74491..75351)
                     /locus_tag="PLP02_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HPF41944.1"
                     /translation="MRSEQEIYDLILHIAKEDERIRAVYMNGSRTNPEIAIDDFCDYD
                     IVYVVTNTLPFIKDKTWLKIFGELLMLQEPDSNDLAFGQKNDPTRSYAWLMLFKDFVR
                     IDLQIQSIEAMQEEYGLDSLTVPLFDKDGILPKISLSNNRDYLIKKPTKAEYAGCCNE
                     FFWCLNNVAKGIARDQMPYVIRMYYETVHKELNRMLAWHIGVTNDFAVSVGKWGKYFN
                     RYLSEDCYNRYLLTIPDGNYDHIWKAIEESCRIFRNTAKSVAKYLGCTYNEQDDKNMM
                     EYLDFVKARL"
     gene            complement(75375..75944)
                     /locus_tag="PLP02_00335"
     CDS             complement(75375..75944)
                     /locus_tag="PLP02_00335"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HPF41945.1"
                     /translation="MIDTKILPEGYVMNYEIDMKHNKKLIVWINIMSLVLLLPFIPFI
                     IRFFSDVMNASMTKLYWLTLLVLGLFLMIIIHEAIHGLFFHVFTKSKVKYQFHGWAFS
                     ASAPGHYLSKWQYFVVGIAPAVILGTLFLVFCFASDIYLAGFAVTMLGMHFCSCIGDF
                     YVIWKLMKYPKDAMVRDTGVGMEFYVREQ"
     gene            complement(75965..76315)
                     /locus_tag="PLP02_00340"
     CDS             complement(75965..76315)
                     /locus_tag="PLP02_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019240210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HPF41946.1"
                     /translation="MKGLLIRTPWIDYILDGKKTWEIRGRNTTIRGRIALIRSQSGLV
                     VGEADLVDCIPLDLSEYANTSDKHMVPKTEIDCLPYPSTYTWVLTNSKRYPEPIEYKH
                     PSGAVIWVNLDNCL"
     gene            complement(76330..77127)
                     /locus_tag="PLP02_00345"
     CDS             complement(76330..77127)
                     /locus_tag="PLP02_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018143005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPF41947.1"
                     /translation="MIGAIIGDIVGSVYEFNNIKTKDFELFKKNCFFTDDTVMTIAIG
                     RALLDWKNKYGELPDKAKESMRKLGRIYPNAGYGHHFYRWLFSDDPKPYGSYGNGASM
                     RVSACGVVGKTLQEVIDISRMVTEITHDHPDGIKAAEATAVAVFLARNGKSKEEIQKH
                     IIDNYYPLDFTLDGIRKDYVFDATALGTTPQALKAFFESTCFEDAIRCAISIGGDSDT
                     IGAITGAVAGAYYGIPREQSDKAREYLDDFLLHLLEDFEKAYPPKII"
     gene            complement(77124..78143)
                     /locus_tag="PLP02_00350"
     CDS             complement(77124..78143)
                     /locus_tag="PLP02_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41948.1"
                     /translation="MSEQFEIKRMGVVLTPSYRECMKALKDMYVPVWKTDKNTYTGFE
                     GEVFESNLPMQIGIGHSGLRVNMIELMRPEGYYTSAQVVDASYKDIQNELVKEYSQPD
                     FKNEYSFDDATTIMASRWETPELIIDHVATKGDNLSEYVHIFFRSNLNRKKRVPIIDF
                     IIPITGGLLLTFLLRIFLWQRTGYAWQLYVLYIFSGLVFGLLLFFALFHEKNPRFRQF
                     LYRKRDELEVDYEAEFPEPFLKKYNGYMFMGKHFLDKAMPLTIYFFQNHILFVSLHNK
                     DIIELDRPFSDAGCLLAEGNNMTAIFANKEFVAFVLENSDEILDLLKEYQDGKRRNAK
                     DGIRV"
     gene            complement(78206..79081)
                     /locus_tag="PLP02_00355"
     CDS             complement(78206..79081)
                     /locus_tag="PLP02_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3737 family protein"
                     /protein_id="HPF41949.1"
                     /translation="MQIQSKEIIENRHYDQERSLYNLTATKLVKCEFKSAEGESPLKE
                     ARDIVVENCLFAMRYPLWHTKGLAMTGTIMEESTRAPLWYVKNGTINNLTLHGTKAIR
                     ECHDIKITDSRIVSDEFGWKSSKISVENSQIQSEYLFLDSTHIDIKNIEMKGKYSFQY
                     TKHVTIDKSHIDTKDAFWHCDDVVITDSVITGEYFAWYSKHLTLINCKISGSQPLCYC
                     KNLKLIDCEMVGCDLAFEYSDVEADIKGRIDSIKNPKRGHIVVDSYGEVIHDNTAIPC
                     KGKVILRENNNHKAK"
     gene            complement(79274..81010)
                     /locus_tag="PLP02_00360"
     CDS             complement(79274..81010)
                     /locus_tag="PLP02_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPF41950.1"
                     /translation="MASKKNKEFLPEWVTTEASKDSYRSISRWGDPKFVKYPKASLYR
                     YMKEKFHMTDDDFSHYHNDIGFDKITDVIPSNMDNEAIDALQKIVGIHNSSYETYDRL
                     SVMYGKTLYDIMRIREHRYDSVPDLVLYPSTTKEVEAIVAYCVSHKIPLYVYGGGSSV
                     TRGVEPVKGGVSLDLRRNFNKVLSFNETDQTITVQPGMSGPDLEKHLNNAIAEFNAKR
                     AYTCGHFPQSFEYSSVGGWVVTRGAGQNSTYYGTIDDIVLSQKYATPIGTINTSHYPK
                     ESTGPNLNQIMMGSEGAFGVLTEVTLKVFRYMPKNRKRYSYMFKTWEDAMDAAREMMQ
                     SEAGHSSVFRVSDAEETDMMLKLYNVDDSPLKFFLKGFLPMQRSLMLGFTDGELGFSK
                     NVRHNIAKVARKYHARSLTGLVCKGWEHGRFTDPYLRDTLLDFGIATDTLECSVNWSN
                     MAKVHDEVRKVCHARPDTVVTTHLSHAYPQGANLYFIFIGHIEKADEFKAYHATILDA
                     IQKSGAALSHHHGIGKMFAPWLEGYLGRKEYGVFKVLKDYFDRDYLMNPGGTIGLDVK
                     EEDKKFLRDNIK"
     gene            complement(81020..82558)
                     /locus_tag="PLP02_00365"
     CDS             complement(81020..82558)
                     /locus_tag="PLP02_00365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPF41951.1"
                     /translation="MDPLVLTFDFGTQSVRAMLVDKKGKILVIRKEKYDIPYYARKMG
                     YCEQSFDKYWFFACKASLGVKEISGKLWDDIASVTVTTIRDTFTCTDESGKPLRDFII
                     WLDQRKAKCEKRLPLLQRLLYKLVGMDYAIECQREICRPNWIKENEPELWRKTAKFGN
                     LSALVHARLTGVYKDSSAAMIGHVPFDNKHKQWMKKSNLQFPIFGIEEDKLTELVDPT
                     DLIGYITKIASEETGLKEGLPLFASGSDKGCETLGSGVMDGHSASLSFGTSATIQFST
                     DKYIEPATFLPAYPAINPKLYNPELQVFRGCWMLSWFIDNFAKDDAKKAYEKGISTEE
                     ELNMHLKDVPIGSGGLMLIPYWNAPLKLPEARGAVIGFMPDHDKYYLYRSIIEGIAFT
                     LYSGFVGLKKRTKQDIEYLVISGGGANSLEICQMAADIFGLPVKKTTEYEASGIGSSI
                     CGFVGLGVYDNFDDAVKNMVSYEKTFMPDENRHEEYMQIYDRIYREIYPALRPIYKKY
                     LRVR"
     gene            complement(82583..82942)
                     /locus_tag="PLP02_00370"
     CDS             complement(82583..82942)
                     /locus_tag="PLP02_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPF41952.1"
                     /translation="MKEITCIVCPNSCLLKVEEKDGKYIVTGNRCVRGIAYGTDEMTN
                     PKRTISSTVKTIFSDVPVVPIKASADFPKERIFDVMKEINKIVLDKRVGRGYKVIENC
                     LGLNIDIVITSDILLNG"
     gene            complement(82948..84186)
                     /locus_tag="PLP02_00375"
     CDS             complement(82948..84186)
                     /locus_tag="PLP02_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF41953.1"
                     /translation="MNNYDTVVIGGGPAGLAAAISAHDNSAKVFLIEREKRLGGILKQ
                     CIHDGFGLIYFNEKLTGPEYAEKFIDEFKQRNIEATLQSFVTKIEKQNDLFMIKYVTE
                     KGVMEVATKTIVLSTGCRERTSRQIFINGTRPYGVFTAGTAQYFTNIAGCLPAKKCVI
                     LGSGDIGLIMARRLTLEGVEVVGVYEILPRPSGLTRNVCQCLEDYDIPLYLSHTVTKL
                     YGENRLEAVDVARVDGNMQVIEGTEKHIPCDTLILSVGLIPENELAETLSVPIDPKTK
                     GPVVDENCMTGIEGVFTCGNAMQVNDLVDYVTITAEKAGKEAATYQKKVREYIPVSAS
                     EDFMYVIPQHVSIGAKGKVDFYFRSKAIEKDVTLNIESEGRAIYKRKYTHLRPPEMEK
                     LSIALDFSQINGSISFTLAR"
     gene            complement(84179..85894)
                     /locus_tag="PLP02_00380"
     CDS             complement(84179..85894)
                     /locus_tag="PLP02_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF41954.1"
                     /translation="MKISRLRKKLAGLYPGVEAEEKDGCVRLTGELDKWEDVVNAGYL
                     AVDKKRYIGVLNDITLKNAKEEKMRLPFICDNKLDGLKPDFLVIGGGISGASILRELM
                     RYDVSAMLVEKESDLAMQASGRNDGEIHPGVDLGIGTKKQYYVVRANRIFGDICDELG
                     VHFERRGQYACFNKPIKPLLRLIAKSRSKIGCPTEVAGKEEILKTEPKFNPEVECAVS
                     NPQSGVVSPYELTIAFAENAVKNGARVELDTAVLSMELKDDKVVSVKTNRGMIYPKVL
                     INAAGTFADKIAEMADDRYFTIHPRSGSDYIFDKKIGQEINHITSIEFISHNKKTAHS
                     KGGGVIKTIENNILIGPNAYETPERENFATESQSLDQVFQKQKYIVPTLEKRDIIAYF
                     TGTRAATYEEDFYIEKGRKTRNIVHCAGIQSPGLTTSPLVAKDVAKMGIDILKESREV
                     RSNTKFDPKRKPDPVLRFMTEEEKTALIKRNPAYGRIICRCEGISEGEIIDCLNRNIK
                     VPTVNGIKRRARPGMGRCQGGFCMPLVAKIISEHEHIPLEEVAGKEIGSPVVFKSQKG
                     GSVHE"
     gene            86011..86583
                     /locus_tag="PLP02_00385"
     CDS             86011..86583
                     /locus_tag="PLP02_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF41955.1"
                     /translation="MAKKRLDENLRMLILSKTQELLQSKSFSDISLNNIAKYSGISKG
                     TLYYYYKSKDDIIVEIYGQSIDSLWDEFIEWVTNKDKNTSLHRVAKFVIMKGTGSSSI
                     RLNLISDAANGNNELKKRLLEKYNAFVNAFYNKLREDMTEEDARFVSWLLVIVTDGVN
                     VQKSLHNPKFDSESFIRMAEDFFLKAEESL"
     gene            complement(86659..87423)
                     /locus_tag="PLP02_00390"
     CDS             complement(86659..87423)
                     /locus_tag="PLP02_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF41956.1"
                     /translation="MKAYLAFVKKEWTELFRTYKLLILLIVFLLFGLMNPLIAFYMPE
                     ILAAAGIDPSTLGLADPTALDSFAQFFKNVGQMGLIVVVIVFANTMAGELSKGTLVNV
                     LTKGLKRRTVVFAKFTVASITWTIVYFVAFAITYFLTDYLFVIGSFSNGFLAFFGLWL
                     FGLFLIASVILGGILFSRMVGSLLFAGGLVVVMTLLNIVPTFQKYVPATIASVNVSLL
                     MGTLETSDFVPALIICGALIVGFVSASILIFDRKQV"
     gene            complement(87420..88325)
                     /locus_tag="PLP02_00395"
     CDS             complement(87420..88325)
                     /locus_tag="PLP02_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF41957.1"
                     /translation="MDILSVKNLTKSFNGIKAVDNLSFEVPAGTIFGFVGPNGAGKTT
                     TMKMILGLLKPDEGKAIIDGMEVKYGETRTNEVVGYLPDVPEFYSYLKPKEYLKLCAD
                     ITGMPSDLFIIRQQELLHLVGLDNVNKKIGSFSRGMKQRLGIAQALLNEPKLLICDEP
                     TSALDPVGRKEILDILLTVKGRTTVIFSTHILSDVERICDHVVVLKEGKLVLSGSVSE
                     LKNKHHNDRLFLEFETEAEAALFLKKSPFAIIGQLDRHFLIGAENIEKTKADILNWLV
                     KADLYPKKFEVLEPTVESLFLEAVK"
     gene            88687..89775
                     /locus_tag="PLP02_00400"
     CDS             88687..89775
                     /locus_tag="PLP02_00400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPF41958.1"
                     /translation="MPKFTGSDMKKRLLSLFLIFVSLFLFSCKSVTDTSFTTTTRNAD
                     EAILLSNLDTIFEDAADISNIYGISVYKNDEFIASEFYGLTTKESKNNVYSVTKSITS
                     LLLGIAIDMEYIESVDQTIGDFIDLSSYENQEVLSTITIKNLITMSAGLVWDSSNLAG
                     EMSELRTAEDPLALILERDIEYIPGTNFNYSDGAAHLMSIIISQATGMSAHDFAKEYL
                     FTPLSIFNTIWNTDRLDNNIGGCDLYLSHEDMYKIAKLVLNRGEYEGTRIVSATWIDE
                     STATQIPVDYATDYGFYWWITSPGDQKRISARGWGGQQIYIVPDENIIVITSADGWVS
                     DNIAQTQFTALHSVAENQIIPLLTSYDD"
     gene            complement(89841..91166)
                     /gene="rlmD"
                     /locus_tag="PLP02_00405"
     CDS             complement(89841..91166)
                     /gene="rlmD"
                     /locus_tag="PLP02_00405"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPF41959.1"
                     /translation="MPELYEAEMINDDGYAVLHKNRKRYLIPNLLLGEKAEIRFFANG
                     FGKVLSRQNDSPDRVKPLCPYYNECGGCQLQHLSYEKQLELKTAKVRHLLANAKLDSN
                     LCLPIIGMKEPFHYRNKAQMVISEKGKKVMGGFYAENTHEIINVDKCYVQNDIANDIV
                     KTCRTLMQQMHIEPYDEDKKTGLVRHIYVRTAEETDQILVAIVTITEMFPGRHNFVVA
                     LREKHPEITTIVQNINTRTTSTVLGNFERVLYGPGSIVDIVLGKKFKISANTFYQINH
                     KQTEILYRKALELAKPKKTDKVLELYSGIGTIGIIFSDHARKVEGVELNKESVKNATQ
                     NARFNNVRNIHFYQMDAAEFIGAIDPEENEFDILLVDPPREGLSEKCLEGIVRLHLDK
                     ICYISCSPDTLARDLSVLAKQGYSLKQVQPVDMFPETCHVESVAMLELR"
     gene            complement(91178..91975)
                     /locus_tag="PLP02_00410"
     CDS             complement(91178..91975)
                     /locus_tag="PLP02_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF41960.1"
                     /translation="MNRVIVITGASSGIGLAMAKRFYDAGDIVYGVSRHYPTTVYDFK
                     YLLCDLTKEEEIKELVSKIMEESGHVDILINCAGMGISGAIEETPTEQCVKIFESNVK
                     SVFMLTREMIPLLRMGTKPRIINFSSVAGEIVIPFQTFYSMTKAAIEIFSTGLSMELK
                     PEKIQVSTVMPGDTKTGFTAAREKNPSVKDSVYHRRLEKSVRKMEHDEQNGVNPDYVA
                     RIVQRLAKRRRMPVVRTVGIGYKILVFLNRILPRRLARWIIYLIYGN"
     gene            complement(91968..93188)
                     /locus_tag="PLP02_00415"
     CDS             complement(91968..93188)
                     /locus_tag="PLP02_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF41961.1"
                     /translation="MDLFKKCFEFTTVDDYKKIGVYPYFHALDSKQDIVVTMEGKRRI
                     MIGSNNYLGLTGDPRVIEAGVKALKEFGSGVSGSRFLNGTLTSHVELETELARFLNKE
                     ACVTFSTGFQTNLGIISAIAGRNDLIVCDRENHASIYDGARLSYAKMVRFHHNDMEDL
                     EKCLQSATEDQGILIVTDGVFSMSGDICNLPEIVRLAKKYGARVMVDDAHGFGVLGKR
                     GRGTAEYFGLEKEVDIIMGTFSKSLASLGGYMVGDEKIVEYVKHKSRPFIFCAAITPT
                     SVATALEALRILESEPERPKALLDIAAYVRKGLIANGIKIMENSIAPIIPIYTYSMLR
                     TLIACKLLYERGVYVNPVLPPAAPEGECLIRTSYTATHTEALMDEAIEIIADVLNNLP
                     ESDEELMKEYVKNE"
     gene            complement(93217..93921)
                     /locus_tag="PLP02_00420"
     CDS             complement(93217..93921)
                     /locus_tag="PLP02_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41962.1"
                     /translation="MLREIRKKIADILMMDDLAPFEDETKENEMVEVGEEYEYEEIFT
                     DLFISVRNLDAFYGDKSIPKAALLLESFLMESLVLLKKDPDVVYVESGDDFLKSTSFT
                     PESEGVMRAFENCVLINTMLDSYNEALKELDCDQIDAGIALAAYPKEEKTEPEEGGDE
                     YTDYPEYGIDRFDTAERLSWNANTGELDILAVNDMAYAMLLDEDKLFFETHLSKASQN
                     VEDITVYTGNIVADED"
     gene            complement(93946..96159)
                     /locus_tag="PLP02_00425"
     CDS             complement(93946..96159)
                     /locus_tag="PLP02_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF41963.1"
                     /translation="MLKLQNISKSYTIGDFTQKALDNVSLDFRENEFVTVLGPSGCGK
                     TTLLNIIGGLDHYDSGDLRISGKSTKDFTETEWDMYRNNTIGFIFQSHNLIPHLSVLQ
                     NVELGLTLSGVKAKERKERAMALLEKVGLADHINKRPNQLSGGQSQRVAIARALVNNP
                     DVVLADEPTGSLDSVTSVQILDLIKEIAKDKLVIMVTHNAELAEKYSTRIVRLKDGQV
                     TDDTNPVKTESDLKQELVLKKTSMSYKTAIISALNNIKTKLGRTLLTAFAGSIGIIGI
                     ALILSLSNGMDQQINDFERETLAGYPITISDSRLDIDKMMSAYEDNMEAYPDTEYATA
                     YDENRLANYFVSNVLTDDYITYVRNYIDNIAPDEIRGVKYVHNINMTLLRYDETASSY
                     VSVYTESKDASTQTSTSIFSFTSKFFTLLPDGEVFSDNYDLIYGSMPENDSVNGKFQV
                     VLVVDEYNRIYKTTLSSRLGFDTDNETEFYFSDIVGKDFTLYVGEYDETTDVSNAIDI
                     EISGIIRIKEGSSVNLLYNGFGYDSSLVDYIEANYPEEIGDVNSIYIYPNDFDAKENL
                     KTYLDAYNDQFDADSISRIEYVDQAATFVTMVQGVINTISIVLIAFAGISLVVSSIMI
                     AIITYISVLERIKEIGVLRALGARKKDVSRVFNAENLVIGFGSGIVGIAIAFGLIIPI
                     NIIIEHFADMPNVAKLSLLHAVFLVAISIALAYIAGLIPALMAAKKDPVVALRTE"
     gene            complement(96153..96767)
                     /locus_tag="PLP02_00430"
     CDS             complement(96153..96767)
                     /locus_tag="PLP02_00430"
                     /inference="COORDINATES: protein motif:HMM:NF037674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF41964.1"
                     /translation="MPKTTFANLPVEKKEKILASARKEFSSTAFEDVSINKIVKGAEI
                     PRGSFYMYFIDKNDLLALLLEEFRNQFVAFLSKKASRSKGNLHEMVVGTHDFIVLASH
                     NQENCGLIRNFLLYSAKNPEHIANRFFHNMMDNFIKLIDTSSFKEKDERYIREVVESS
                     FAILKETIHQTLVMNRPVKESHECFERHLAILENGYLIKENESC"
     gene            complement(96914..97555)
                     /gene="fsa"
                     /locus_tag="PLP02_00435"
     CDS             complement(96914..97555)
                     /gene="fsa"
                     /locus_tag="PLP02_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPF41965.1"
                     /translation="MEFFIDTANVDDIREAASWGIICGVTTNPSLIAREGRKLIDVIK
                     EITDLVNGPISAEVKEASAEEMVEEAFGYARLHPNIVIKIPMTFEGLKAVKVLSSNRI
                     PTNVTLVFSVPQALMAAKAGATYVSPFLGRLDDYTKSDHAGYELLEDIRLAFDNYDIQ
                     TKIIAASIRHPEHVTQAALAGAEIATIPFKVLKEMIEHPLTTKGLEIFRNAGK"
     gene            complement(97577..98218)
                     /locus_tag="PLP02_00440"
     CDS             complement(97577..98218)
                     /locus_tag="PLP02_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPF41966.1"
                     /translation="MKMIDNEVKQFLNAVDSPAPAPGGGSVSALVSALGVCLLRMVGH
                     LTIGKKKFSAFSEDEQKAFLDEFARLEGIRGQLEEEIDKDTIAFNKVMAAYKLPKETE
                     EEQAMRINAIEMATKEAIETPYQAAVAAASALSGLDLLLKMGNKNAISDLGVAVLLLA
                     AGIEGALFNVRINLPVLSDEKLRNTYKEQCDKILAYTNLQKTDLLNKVYKILV"
     gene            complement(98249..99484)
                     /gene="hutI"
                     /locus_tag="PLP02_00445"
     CDS             complement(98249..99484)
                     /gene="hutI"
                     /locus_tag="PLP02_00445"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPF41967.1"
                     /translation="MADLLIRNIGKLYTPTKTPFTNKDKETTRIISNAFVAVKNGLIE
                     ALGEGDGAFWLEQNTKIFDAKGMIMTPGFIDSHTHLVHAGSRENEYAMKLSGMTYLEI
                     LQKGGGILSTVKATRNATFDELYEKAKKSLDIMLSYGVTSIEAKSGYGLDIDTELKQL
                     EVAKILNENHPVEITSTFLGAHAYPAEYKGKEKEYLEIVKKTMDYVKEKDMAEYVDVF
                     IEKGVFGIEASEDVIKHAKELGFEVRLHADEMNSFGAVPLGIKYKARSLDHLMMISDK
                     DIDLLASSDTVATLLPMTSFYLNEGYAPARKLIDKGAIVSIAGDYNPGSSPSENYLLA
                     LQLAAGKLKMTPEEILASATVNPAYSLNKGNLVGRIEKGMHADLVLLDAPNFSYVLYH
                     FGINHVKHVFKKGKLVYND"
     gene            complement(99496..100461)
                     /gene="ftcD"
                     /locus_tag="PLP02_00450"
     CDS             complement(99496..100461)
                     /gene="ftcD"
                     /locus_tag="PLP02_00450"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPF41968.1"
                     /translation="MAKIVECVPNFSEGRDKEKINRIVSVFDKYQGIRMINCEPDGDY
                     NRTVVTLIGDPETMQKAVLEMVGVAKEEIDLNHQTGQHPRMGAVDVIPFIPIEGVTIN
                     DCVDFAKTLGKNIASVYNIPVFLYAEAASKEDRMSLPNIRKGEFEGMDEKLKDQNWHP
                     DFGPAAKHPTFGVVAVGARQFLIAYNIDLETTDMDVPEKIARAIRKSSGGFQYIQAGP
                     AFLEKYNHVQVTMNILDYKKNPIYRIFEVVKMEAKRYHIEVKSAELVGLMPKDALIRS
                     LAYYLDMDDDIRDPKQLSLAELTELARKYLMFRDFDINKIIESYL"
     gene            complement(100463..>101066)
                     /locus_tag="PLP02_00455"
     CDS             complement(100463..>101066)
                     /locus_tag="PLP02_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPF41969.1"
                     /translation="CQDAIIGKVAGIDNIAISWDSQVPVDRRIQGQLNAPMRSIIAVS
                     SGKGGVGKSTVSVNLGRDHHDTGGTDSPFRETANIKDGSNIMADMATQVALGNASRGM
                     SLVTLHNGGGVGIGKVINGGFGMVLDGSLRVDEILKSAMTFDVMAGVARRSWARNDHA
                     METASSFNQDNNNAYITMPNLVDEKYIHSIVQKEYVKGGK"
BASE COUNT        27018 a        23318 c        21875 g        28855 t
ORIGIN      
        1 cccgtgcagg cagtcagggc aaagaatgta aaagccaata ttcccagtac aagatatttt
       61 ttcatgtttt catctccctc aggatgtctt ggacagtaat tctatatttt ccaaagtaat
      121 gtaactgccg tttctgtaga caacgatgct tatgacatct ccgacatggt acaactcata
      181 gaaatctttc gatgtttcgg ttttgttcga tattacaatg tcaccgatct gggttatgat
      241 gtctcccgcc tgaacatatc cgtccaccga cgaaccggac ataacggcta taacatagta
      301 tcccgcctcg attccttccg gaatggtaat gccttcttgt gcgaattcct ccggcgtaac
      361 agtcgcaata tcagctatct gcactcccaa aaccggagtt tgtttggaaa acccgtacaa
      421 ttcgatgtca gcgcagaccg ccgcgacaag actaatggga atagcaaatc ccatgccttc
      481 gatttccgac gaagatatct tgatggaatt aattccgatc acttctccgt caaggttgaa
      541 aagtgcgccg ccgctgttgc cggaattgat ggaagcgtcg tgctgaatga aactgaaata
      601 catttctgtg atcccgtcat tgtcggtatc gaaatatctt ctggttcccg aaactattcc
      661 catggtcacc gaatcgaaaa attcgtagcc atccggattg cccacagcca aaacaacggt
      721 tcccgtcgct aaatcggaag aatctccaaa agacgccaca ttcaatgttc tgtcggtgac
      781 aaaggtcaga acggccaaat cagtagtttc gtcatagccc aaaattgtcg ccgctatttc
      841 cgtcccgtca cccaagcgga tacggggatc gtttgttatg ccgtcatccg tggcgtcgtc
      901 ctcgaccacg tgatgatttg taaccacata atacattgtc ccgacatgct tgtaaatgac
      961 gccggaaccc accgactgca acaagccgga tgaactgtag ttggaaaccc ctatgatgga
     1021 tgacgcctcg tcagtcgcca ctcgcaaaac ctgttcggca agcgaaacgg cttcgagcga
     1081 caaggtccct tcatccagat cgccatatat ttgggtgatt atctgatcgt agatttcctg
     1141 gaatctttct tccgttatgt cacctatcac ttcttcgcgg atctgctcat agagatcgtt
     1201 ataaagcatg gcatagactt ccgataccaa acgatcggta tcgattgttg tcgtagtggt
     1261 gttggtattg gtggtagaag acgtcaaaaa agtcctctcc gtcgtggtag tgctggaaat
     1321 actgaaaaaa tcacaaccgg caaagacaaa caccagcgtc accaataaga tgaaaataag
     1381 gccttttttc ttcatgtact caacttcctt ccggatatgt gtttccgata ttatatcacg
     1441 atattatgag aaagcggtga aaattgcatt tgtcacatta tttttcgtcg ctcatcacga
     1501 caagtagacg tcccttggaa aaaccgactt ctccggaccc ttcgttcgat acgcaaagcg
     1561 agtcgctggt tgatagataa taattgttca agctgtattt gaagccggtc agagacaggt
     1621 tatagcaatc ctcgatggca aaaaaggaaa tgtatctgtg gtagttgtca atatggtgtt
     1681 ttcctttgcc aagaacaaaa atatgatgct ggttatcgcg gaatgacagg tcgaaacgtt
     1741 tcagcaggtt gatgtttgcc agcaaatggt ctattcttcc tccgataccg ccgtaaattt
     1801 ccaaagaagt gtaatcggta ttgttgtaca agtaatcgat ggcataagca agatcggtca
     1861 tcgacttttc cttggacaga gtgatgagtt tcctgctttt ttgttttacg gaatcgaaaa
     1921 agagcggatc gagggaatcg aaatcgccga tcgctagatc aatcatgatg ttgttttcca
     1981 caagatattt cagaccggaa tcaactccaa ctatgaattc atcggcttct tcaaaataaa
     2041 gacgggaaaa atcatagttg ttgggtccgg caaagatttt gatctttcta gacattctcc
     2101 aacaccccaa tcgctttttc ccggtctttc tgattaatga tataggatcc ggccaccaca
     2161 atgtcggccc cggaagtttt tgccatggcg gcggttttgt cattgattcc gccatccact
     2221 tcgataagga aatgatagtt gtttttgctt ctctggtcgg cataataccg gatcttgtcc
     2281 aaggcctccg gcatgaattc ctgaccgccc ttgcctggtt cgacgctcat gactaacact
     2341 aaatccagat ctttcaggaa aggatcgagc aaatcggctt tggtggccgg tttgattgac
     2401 atgcctgaca aacagcccct ccctttgatt tttgcaatca gattggcgga tttttccttt
     2461 gcggcttcgt aatggaatgt gatcatatca gcgccggcat caatgaaatc acgatacctt
     2521 tccaggggat tggcaatcat cagatggcaa tcgaaaaact gattgctaaa ggcttttact
     2581 ctgtctagca tttcttctcc gtaagtcagg ttgggaacga aaattccatc catgacgtcg
     2641 aaatgaagcc atttcgcttt tttcaaagaa acaatctcgg tttccagttg ggaaaaatcg
     2701 gcggttagaa aagaaggagc gcagatcatg atgtcatcac cctttcagta tttaggcttg
     2761 attgccttta tttccgcata aaaacggcaa tagttgtcat atctttcttt aagaatggag
     2821 ccggcttcca cggcttcttt caccttacag tccggttctt tcaaatgccg gcatccggaa
     2881 aaacggcacc cttcggacaa attgacaaaa tcaggataaa ggttttgaag cgaatccggt
     2941 tcgatatcga tgaaatccag tttggaaaat cccggcgtgt ccgcgatcca tccgtctccg
     3001 aactcgataa gttccacgtg tcttgtcgta tgcttgcctc ttcccaaagc ttccgaaata
     3061 tcgtctgtct tcaattccag tgttgggttt aaggaattca acaagctact ttttcccgct
     3121 ccggtctgac cagcaagaac attcgttttt cctttggtca gtttctttat ttcctccagg
     3181 ccttcattgg ttttgttgga aacaaaaacg gtggggaaaa aagatcggta atattcggcc
     3241 gttttctcca aggtttccct ttcatctttt gtcataaggt cggtttttgt tattattatc
     3301 gctggcgtta ttctgctgtt taaaatcaga attaggaatc gatcgagcaa ctgtaagctg
     3361 aattccggac gtttggcaga cattatcagg agcacctggt cgacattgca tatcgacgga
     3421 cggaccaaat cgttcgtccg tgtttcgacc gacaggatcg attcatcgtc gaaagaaacg
     3481 atatcgccca cttttggacg ctcgttctga tagcgaaaaa tacccaacgg cttgacatca
     3541 cgacgttttc cgtcgcaacc ggcgaccgta taccaaccgc cgatcaattt gatgatgcga
     3601 cctttctcca aatgcatgcc tccttggaaa gataccataa ttatacagga aaaagcaaaa
     3661 aaaaagaaga cttgcgtctt cttgaatcag taatttttca cgtagcgttg gaaatagctc
     3721 tggtcgtgat cgcaaaccgg gcaaaccttg ggagcctttt tcccgacatg gatgtgtccg
     3781 caattcgaac agatccagat ttcggaatcg ttgtccgatt cgaagatcct ttggtccttt
     3841 atactcgcaa gcagggcaag atagcgcttc tcgtgttcct tttcaatcgc cgccacagct
     3901 tcgaacagat aggcaatctt ggtaaagcct tcctgtttcg ctacttcggc gaagcctttg
     3961 tacatgtcgg tccattcgta atgctcgccc gccgctccgt cttccaaatt tgcttcggtg
     4021 gcgggaatgc caccgtcatg aagcagtttg aaccatactt ccgcatgttc cttttcgttg
     4081 gctgccgttt caaggaaaaa gttcgctatc tgtacgtaac cttccttttt ggcttttgaa
     4141 gcataaaaag tgtatttgtt cctcgccatc gattcgccgg caaatgcctg ttgcaggttt
     4201 tgttccgttt tactgccttt gagttccatt atgtttcctc cttataaaga ttgtcggatt
     4261 ttcagtctcc gaatgatatt ccgatatttc tggctgcttg gacaagatcg ccttcgggat
     4321 caacgagcct ggctccgccg cggcttgttt ctccggtttt tccgtgcccg acgacatctt
     4381 cgattggaac catggtcatt ttgtcattca tgatcgttac catgtttccg aattcgccgt
     4441 tggcgatcat atcgacggca tgcgatccat acttggtgcc aaggacgcgg tcgaatgtgt
     4501 cggtgacgcc gcccctttga atgtatccca gattggttga acggatttcc tgtccggtaa
     4561 tcggctggat cagtgtttcc agttgcgatg ctatacgggc tccgatgcca ccgagtttga
     4621 cactgtcggg gctcttgttg tcgattgttc tgatcgtcag ttctccgtca atcggtttag
     4681 cgccttccga aacgcaaatg atcgagaact tgctgccgta attgtatctg tcgatgatct
     4741 gtttggcaac ctttttgata tcataaggaa tttcgggaat cagaatgaca tcagccgatc
     4801 cggcgattcc gccttcaaga gccagccatc cggcgtggcg tcccatgact tccagaatca
     4861 tgacgcggtc atgggaaaaa gctgtcgtat gtaaagcatc gagcgataac atgatatggt
     4921 ctagagcggt acggtagccg aatgtgatct cggtgtcggg aacgtcgttg tcgatggttt
     4981 ttggaatgcc gacgacattc acgcccttgc gggaaaaatc gcgggcgctt gtcaaagtgc
     5041 cgtcgccccc gatgatgata atcgcatcga cgccgttttt acgcaaattc tcgatcgcga
     5101 tatcggaaac atccaaatag acatagttcc cgttgctgtc catggtccgg taattgaaaa
     5161 ggttgtcgcg attcgacatt ttgagaatcg tgccgccttg atggaaaata ccggtgactg
     5221 ccgagagcgt caaatcgata taatcgtcct tgaagagtcc gtagtaacct cccatgtagc
     5281 cgatcacttc gtatccgtat ttgagaattg ccgtcctgac ggcggctctt atcacggcat
     5341 tgagtccgga gcagtcgcct ccgcctgtca aaattgctat ccttttgatt tttttattcg
     5401 catccattgt ttattctcct gaaaaaaata tctttattag tgtatcatca aaaccatatt
     5461 cagtcaaatg gattgatatc attttgtttt tgatttcatc cttaactgac cgcaagcggc
     5521 atcgatatcg ccgccttgtt cttttcgcat cgtcacattg atttttcttt tcatgagaca
     5581 atcgagaaat atggatgtcg cgtcatgtcc gctgggatcg aattcgaatt catccacagg
     5641 gttataagga attagattca catagcaatt cacatctcgc aaaagatcag ccagtttgtt
     5701 tgcctgttcc aaagaatcgt tgacgccttt tagaaggatg tattcgaaag tcacccttct
     5761 ggaagttttt tcgaaataat ccttacacgc ccggatcacc tcggcaaccg gatatttccg
     5821 atttatcggc atgagacggt tacgaagagt atcatctgaa gcatgcaggc taacggccag
     5881 attgctgcga atcggttctt cggcatatcg tctgatcatc ggaactatgc cgcttgtcga
     5941 tacggtaatg tgccgttggc cgatcgccag acctttgctt tcattgataa tgccgataaa
     6001 cttgatgaca ttttcatagt tgtcgaacgg ttctccggtt cccatgacga ctacgttcgt
     6061 gatcctgact ttggcttcca aacccgccat gataacctgg ccaactaatt ccgctgtatc
     6121 cagattcctg attttatttt ttaagccgga agcgcagaaa gcgcatttca tgttgcagcc
     6181 aacctggctg ctcacacaga cactgtatcc gtaatcatgg gtcatcagca ctgtttcgat
     6241 cagacttccg tcattcatcg caaacagata ttttctcgtt ccgtccgatg aattctggat
     6301 cgtcgcgatt cccgggagac caaaaccgaa atgatcgctt agatattccc gcaatttcag
     6361 cgacaaattg gtcatcaaac caaaatcgcg gatgtttttc tgatacatcc actcgaatat
     6421 ctgtcttccg ttgtactgtt tgaaaccggc ttccgacaac atgattccta gttcttgcaa
     6481 ggagaggccg tatagattgg ttttttccat ggggtttcct ccgaaaatcc gatggctgaa
     6541 tgttacgtaa tgattatatc acatctttga tatgtctatg ttccgattcg cttcacttct
     6601 taataagaaa acggtcccgg aaaggaccgt ttgtgttttg taggttattc tctcaatgtg
     6661 gcctgacatt tcttggcaag ttcgcccact atcttgttca cgaaattgtc gacatcggtt
     6721 gtcgaaaggg tttttccgtc atcttggaaa accagggata gagcaatcga tttcttgtcc
     6781 tggccaagtt tttctccttc gtagcgatcg aatatttcca caatccgaac ggatttcatg
     6841 ttcatcttgc ggatggtttc caccaaatca ccggccggga gattcttatc cacgatgact
     6901 gccagatccc gtgaaaccga cggatatttc gataatggtt tcataacgat gttcttattg
     6961 ccacatccaa atattcttcc aagattcaat tcaaatgcgt atgtttcccc gatttcctcg
     7021 ctatgttcta actcaggatg gaaacgacct aacacgccaa tcttttttcc ttttgatgta
     7081 acattagcga cgactcccgg atggaaacca ggtaattttt tgtccggtat ctggaagtcg
     7141 atatcctcta gaccaagttt cttaagaatt gcgatgatga tgcctttggc cagatagaag
     7201 tcgatatttg cttgatcgtg ttgccacttc gacatccgat aagaaccgtt catgatgccc
     7261 gataaaaatt cttcctcgcc ttcatcgaaa tatcctttgc cgatttcaaa caggcaagcc
     7321 tcatcttgct tatgatcctt gttatatttg aggacactta taagcgatgg caacagtgaa
     7381 tgtcttaaac tagccctttc ctctgaaatt ggattgagta ttttaattac ttttttgttg
     7441 ctgtcatcga acattctggc attatgttcg tttgtgagag tgtaagtcgt tacttcgtcg
     7501 aatcccaaat ccaacatgaa cgatctgagc atccttcttt gtttttggat atcggtcaaa
     7561 gtgcctgtcg ttatggtttt tggtaatgtc atcgggatat tgttgtatcc gtttatgcgg
     7621 acgatttctt cgatgacatc ctggtatgtc ttgatatcct gccttcgcga aggagcgtaa
     7681 acggtaatgt cgtcatcggt aatcttcgcc cggaaattca agcgttcgaa gacatcttcc
     7741 agttcttttt ccgtatatcg cttgccggtg actgactcta tttttgccat tgtcgtcaat
     7801 atgacggtgt cgccagcgtc gttgacatca aaataattga ttccgcgtaa tatcctgcca
     7861 ccgcaatatt ccctgaacag ttcagcggca cgattagcgg ccaatctggt acgagctggg
     7921 tccaagcccc gttcgaaacg gttggacgat tcgctctgga gagcgagacg cttggatgtt
     7981 tttctcaccg cataaggtga aaacgtcgcc gactcaagaa gaatcgttgt ggtattgtcg
     8041 tcaatctcgg tttccaaacc gcccatgact ccggccaggg cgatcggaac attgccatcg
     8101 gtgatgacga catccgcggg atcaagttct cttttcttgc cgtccaaggt gacgatcaat
     8161 tcgccttctt tggcatttct gaccaagatc tgtccgctta ccagtttatc gtagtcaaaa
     8221 gcatggagcg gttgtccgta ttccagcatt acatagttgg tgatatccac gacattgttt
     8281 atgggacgga tgccgcatgc tatgagacga gattgcatcc atctggggct caatccgacc
     8341 ttgacatcct tgatcaccct tgcataatat gattcgcaat cggaagtctc cgtaaacacg
     8401 caaaccggat tcttctcctg atcctcttcg ggatccggat ttgacacatt gacagattca
     8461 tttagaacgg ccccaagttc ataggcaact cccaaatggg acaagagatc ggcccggttg
     8521 ggggtaagat ccaaattgag aatatagtcg tcaaggctta accatgccaa aggatcgccg
     8581 cccagcggcg catccgagga caggacatgg attccgtcct cctgatgata tttgtgatcg
     8641 attcccagct ccgacaaaga acagatcatt ccgttggatt cgatacccct gatctttgcc
     8701 ttcttaattt tcaggcctcc gggaaggttg gctcccggca aagcgactat cactttctga
     8761 ccggcttcaa cattgggagc cccgcagata atatctaatt tttcgtttcc ggtatcaacc
     8821 acgcagacat gcagatgatc ggaatccgga tgcataacac aggtttccac gaagccgatc
     8881 gccaggttgg tggcttcgac cattttttcg agtccttcga cttcggcgga catcagatta
     8941 agttttctga ccagttcgtc cttatcaata ttgatattta tatagtcttt caaccaattc
     9001 attgacaata gcattttatc acccctctac cgaaactggc ggttgaaccg caggtcgttg
     9061 gtatagaaag ccctgatgtc gtcgattccg tatttcagga tcgcgactcg ttccacgccc
     9121 attccgaaag caaatccctg gtaacgggaa gaatcatatc cgcaagcttc caagacattg
     9181 ttgtttacca tgccggcccc aaggatttcg atccatccgg tgcctttgca catcgaacat
     9241 ccggcgccgc cgcatttatg gcaggagaca tcaacttcca cgctcggttc ggtgaatggg
     9301 aagaaagacg ggcgtaaacg gatttctctt tcttcgccga acatctttct cgcaagtacc
     9361 agaagagttc ctttgaggtc agcaagcgag gtgttctcgt cgacgaccaa agcctcgatc
     9421 tgagcaaatt gatgcgaatg tgtcatatcg tcatcgtctt tgcggtatgt cttccccgga
     9481 catatgactt tgatcggttt gtttcccccg gccttgagca tggtcctgac ctggacgggc
     9541 gaagtatgcg ttctcagaag attgtcttcg gaaatgtaga atgaatcctg catatcgcgg
     9601 gcgggatgca ttttcggaaa attgagcatt tcgaagttgt atttgtccga ttcgatttcc
     9661 ggtccttctt cgaccgtata tcccatgccg acaaaaagat cctcgatttc ttcgataacc
     9721 tggctgatca gatgtttggc tcccacgtac atttggcggc cgggcaggct gatgtcaatg
     9781 gtctcttgtt tgagtttggc atcgactgct tctttatcaa gcctctcctt ggcactatca
     9841 aaaaaagccg tcacttcgct ttttgcggca ttgattatgc caccaaaagc cggacgttct
     9901 tccggtgtca gatccttcat ttgttgcatc attgtattga actcgctttt cttgccgaga
     9961 aaagcagctt tgaggtcgcc aagttcggca ggagttttga ttccttcggc cgccgatttc
    10021 gcttcagcca cgatcttggc cagttgctct ttgatttcca ttgtatcctc cccttgataa
    10081 gacaaaataa aaaaatcgcc cgaaaaaaag ggcgatttta caaaccgtgg taccaccttt
    10141 gttccgggta accgtcccgg cactcaatgc cagataacgc tggcggcgga gatgattggt
    10201 ctcacttggg agatgaattt cccggtcttg acaagagacg ctttcaccat ccgcgtcctc
    10261 tcttgcttgt ctttggtttc gggtacttgt tccccttcat cgcgataaga tttcttgttt
    10321 tgattatatc ataaacaagg tatgaataac aagactaatt tcttcttccg aatatcagta
    10381 gcaaacgcaa aatttccaca tagatccaaa ctacggtaac aagcaatccg agtgagagaa
    10441 tccagcccat tttggcatcg gttccctgat caacgcaatt cttgatgctt tcaaagtcca
    10501 agagcaggaa caaagcggaa agcaaagctg acacgccgca aaccagataa taaatcgtag
    10561 atgtcattgt gaacggcaga attatggata gaaaagccat gatgattact gaaatgagag
    10621 ccaccaccat taccgatgcg aatttctgcg taaccttgat aatgccagta gaatagaaca
    10681 gcagcataat gacaaaaacg attccggtcg tcataagagc cgtaggaatg atgccttcgg
    10741 ccaaatacgc gtatatcgcc gatatataac cgagaaaaac tccttcgcag attgcgtata
    10801 tcacagaaaa aatcggggaa agggtgacgg aacgcgtccc gataatcacg cttataaaac
    10861 caagtaatat ggcgccgatc aggataccga ttccaatctg agccatatac aatattgtta
    10921 aataggcgct tacacccaaa ataccgatga gaaataccgt cttcatcgcc acattggaat
    10981 atgtgaccgg gcgatcgcta acgtaagtgt tggcacgcac ggtacggata accggatttg
    11041 agcttcttaa gagtggcatt gcaatacctc ctgaatcatt gttaatgtag atattataac
    11101 gcttttttgt gggatttata tgataaatgc aatttatttt cccatatcca aatcaaaaag
    11161 cgagatttgg tcttcttcaa tcattccttc aaaaaccccc aagtcttcaa gccggtcgaa
    11221 aatggtctta gacagcttgg ttcgtgtacg gatgtcttgt ttcgaaatga acggcttttt
    11281 ctccctggct tcgacaaccg aattggcggc tgccaaaccg agagaatcca cgacgataag
    11341 tggcaacagc aatgatttct gatcttcgtc gatcacgaat tccgtggcct gtgagcggtt
    11401 gatgtcaatc ggattgaacc tgaatccgcg catgaccatt tccaaagcca cttctaaaac
    11461 ggtttccagg ttcttgtcac gatccgagga atcgttggtg atggaagcca tccgtctctt
    11521 tattccctct tcgccctgat agaaggcatc gacatcgaag aaaggcgccc gtttggaaaa
    11581 atatgccgca tagaaatata tcggtcggta ttttttgaac caagcgattc tcattgccat
    11641 caaaacataa gccgtcgcat gagccttcgg aaacaggtat ttgatcttgt tgcatgacca
    11701 gatgaaccat tcaggcacat ctgactgtcg cattaaatca gtgtaatatc cccatttttc
    11761 gggatttaca tgcgttttac ctttgcggac gaattccatt atttcaaacg cctgtgtcgg
    11821 ttgcatgccg taatcaatca aatcggtcat gatgtcgtcg cggcaaccga acactttctt
    11881 gaaatcaact cgtccgaatt cctttatctt gccggatatc aaatcctgga cgttggaatt
    11941 ccatacatcg gttccgtgcg atagtccgga tattttgacc agttcggcaa atgttttcgg
    12001 gcgcgaatct ctcagcatcc cgcgtacgaa gttggttccg aattccggaa ttccgaatga
    12061 cgccacatca ctgttgatgt cttccggacg caatccgatg gcttcggttc cggcaagcag
    12121 tctgtagact tccgtatcat caaccggaat gtctttggga tcagaaaacg gaaaatcgag
    12181 tggatctttc ttgacaagat ccatcagata ttttatcatt gtcggatcat catggcccag
    12241 aacatcgagt ttgaataggt tgctttcaaa cgaatgatag tcgaagtgcg ttgttttcca
    12301 ggtagtatca gttgagtcgc cgggatattg gatcggggtg acatcgtaaa tttccttatt
    12361 gttgggaaca acgacgatac cgccgggatg ctggccgctg gtacgcttgg atccttcgat
    12421 cttttttgcc agtctttcca tttcggcacg tctaacgatc aaagcaggtt ctcctgcttt
    12481 ttccctgagt tcgttttgtt tttccacata atccctgacc atcgcaaaag ccgttttcga
    12541 tccgcatgtg ccgatcgtac cggctctgta ggcataattg tatccgaaaa gtttacggat
    12601 gtattcatgc actttgcttt gatagtctcc ggaaaagttc agatcgatgt ccggaatctt
    12661 gtctcctttg aatccgagga aggtctcgaa gggaatgtca tgaccgtcct taatcagttt
    12721 ggttccgcat accggacaaa attgatccgg cagatcccac ccgcattcgg cgcggtcgaa
    12781 caaccggttg atcttggttt caaaatcggt tgatccgaat tcacgttcct gctcgtcgtt
    12841 tttcttgaaa gcggaaaaat ggcatttggg acaacggtaa tgcggcggca aggggttgac
    12901 ttcggttatg tccatcaaca tggcgacaaa actggatccg acggatccgc gtgagccaac
    12961 caaatagccg tcatcaagcg atttttttac taacaggtgc gatatgtagt aaatggtgga
    13021 gaactggttg tcgatgatgc ttttcagttc cttgtcgagt ctggtcttca ccaaagaagg
    13081 caatggactt ccatacatgt tttccgcacg ttctcgtacc atcgtctcca ctttggattt
    13141 tatggaaggc actccctgtt ctgccaaaaa ttcgtccgac ggggcgaaca actcgttcgg
    13201 gaatatctgg atccaatcga tcgaatcggc tatcatgttc ggattggtaa tgacaatctc
    13261 gcaagcgaca tcgtcgccca agaagcggaa ttcgtccaac atttccgatg tcgtcatcaa
    13321 gtgttgggac ggtttggttt tttcgttata cagatagtgg tgcccgcctc ccaccatcgg
    13381 agagttgatc aatatttccc gactcttcaa atcttccggg tcgagatgat ggacatcgcc
    13441 ggttgcgcat acgggtatgt caagttcttt tcccacttcg acgatccggc ggatgacatc
    13501 ctggatcatc gtctgccatt cgggtatctg atcgaccaaa taaccgaatg atgacggcgg
    13561 ttggacttcc agaaaatcat aataggacgc ggcttctttc aattcttccc ttgtcttgac
    13621 catggcgatt tcgaaaaaat ccgagttgcg gcatccggaa gaaaccaaaa gacctcgacg
    13681 gtatttgtcg agaactgttt tgacgacctt gccatcccgg tcataaaaat cggtcagagc
    13741 cattgataga atcttataga gatttttgag cccttcctgc tttttgacaa gcaggttgat
    13801 atgcttcgga tatggatatt tgtatacttc cttgtgatct atgatcaggt tgatgtcctg
    13861 aaaacggttg ataccgtctt ttctgatctt tttcaacata tgcaggaaaa tctcggcggt
    13921 tgctttggca tccgatgcgg cacggtgatg ttcgtccagt gtcactttaa ggaaacggca
    13981 aagcgaatcc agcgagtatc taccccgatc cgggtaaaca accttggcaa gcgttaaggt
    14041 gtctatcgaa ggctgaatcc cggcaaatat gccattttcg cgataaccat gataaatatg
    14101 ccccaagtcg aaatcagcgt tatgggcaac cagaacggaa tcctttgtga attcatagaa
    14161 atcacgcaag accttttcca ctcccggagc atccttgacc atttgatttg tgatcccggt
    14221 gagattctgg gtgaaagaac tgatcggttt attgggattg acgaacgaat gaaattcgcc
    14281 gatgtttgtt ccacttttga tcttgacgcc ggatatttcg atgatcgaat cataaagcac
    14341 ggacaatccg gtggtttcga tatcgaaaac cacataggtc gcatcgccca gcattttggc
    14401 agattcccct ttggcaatcg ccactgtctc gtcgttgaca aaaaccagtt ccaaaccgta
    14461 aagcggtttg agattcttat cgtgggtcag tttggcaaaa tcaggataag cttggaccga
    14521 tccgtgatcg gtgacggcta aggcgctcat tccccaggcc ttggcccgtt cggcatattc
    14581 gtcaatcgaa ttgacgccgt ccagggaaga catctttgtg tggacgtgca attcaacccg
    14641 cttttcggca cagtcgtcca ttcttcctgt tttcggcagc acccggtctg atttagccat
    14701 ggaaatggca ttaacaaaga cttcgttgta ataggtgtca aacgaagcgt agccggatac
    14761 gcgcaaaccc atgccttctt ccagggtttg gcagaaatcc ttctcgtcgg atgaagacaa
    14821 tctcttcttt actaaaagcg aatcggtgcc gtcggtgatg atgatattgg cctgggaatt
    14881 gctgcgcatt tccctcactt caatcgaaac aaccactccg gccagattga aattggcttt
    14941 ggccccgttt ttggctttgt attcctccaa accgatttca tcatggggga ccttctcaaa
    15001 cgacatgaaa tcagtgtcta ccggtctgtt gtcgtatagt tccctgtata caaccgttga
    15061 tgaattggcc gatgtctggc ttgatatgac aaacgaacgg ttggctttct cgatctcttc
    15121 gctgatcgtt ttgcaattct ggtctatcga tatcgccact gtcgtcgcaa acccgttgtg
    15181 ttcgagttcg gcctcgatat ctttgcggaa catactcgct gatttggctc cctggggaac
    15241 catgatcttg atcattccgt cgacaatgtc ggtcggatag tctttgagcg gcaaaattct
    15301 tttgtcttct tctgttaata catcaatgac gaaatcataa taatcgagca aatcctggtc
    15361 ggaagtcatg ccaaaaacag tctgatattc aaccgacttc agactgtcaa cttcctgcgg
    15421 tagttttttc agatgatcaa taaaattccg atatctgaca atcggaaccg gacgatcgaa
    15481 aaataaagaa aaaaaccagc tgttttgaac ggtgtcgact tccattttct cgattgttcc
    15541 gtactccagg atgatatcgt catggatgcc aagacgatca agaagttttt tcattccgtc
    15601 gttcaaaagt atcatctccg attccagatt tcatattcta ttatatcaca tgaaaagcgt
    15661 taagtgaggc aaaaacacaa ataaaaacag cctttcggct gttcagaatt cggctttgac
    15721 tttcccggcg aggatttcct caagcgcctg ccccacaggc ttaacgcatt tgttttcgac
    15781 agacgaataa tcgtcttcct tgatgatttt tgcccgtttc gccgcgatat atgccagttt
    15841 gtatttcgag tcaacgactt ccaagagatc gtcgattgac ggataacgca atccttcctt
    15901 gtttctattg gacatttatc tttccctcca tgagtttgtt atatttgtaa atcgatcttt
    15961 ccgtcttggc atgctcggcc ctgatgatgg cgtagatccg gtcggcggcg ttttcgacat
    16021 cgtcgttgac gacgatgtaa tcatacttgt aggccagtat gaattcacgt tccgctttga
    16081 ctatccgttt ctgtatgaca tcattggctt cggtgccgcg tttgatcaga cggtcatata
    16141 aggcttgttt agacggcggt acaatgaaaa taaatacggc ctccggcatc ttttcccgaa
    16201 cttgcagggc gccttggact tcgatttcca tgatgacttc cttgccttcg gataatttct
    16261 tctcgataaa cgatctcgga gtcccgtagt attcgccgac gaattcggca tactcgagca
    16321 tttccccgtt tttgatatgt tgttcgaatt cttcgcgaga aacgaagaaa taatcgattc
    16381 cgtccctttc gccctcccgc ggtttgcggg tagtcatgga tacggaataa cagaaattgt
    16441 tatcggggat ttgaaacagg gccttgcgga tagtaccctt tccgacaccg gaagggccgc
    16501 tcatgacaac caataagccg cgttcgttaa gcttcacagc ctatatcccc ccttgaacgt
    16561 attcattatg caatataata ccacaaatcc cgaaaatagt aaaatttttt ttcgttttac
    16621 cacgatatga tataatgtaa acggtgattc aaatgagtat ggacggcaag ttcatcgaaa
    16681 ggctatgcat tgaactcgac aacaatcttg cgggcggtag aatccagaaa atctaccaac
    16741 tgagcaaaac agattttctg ttcattgtaa gaacgccggg agagaccaaa caactttatg
    16801 tttccgtatc gacttccctg accaggatcc acctgtccgc catgcgttac gacaggccgg
    16861 aaaatcccgg cggtttttgt atgcttctcc gcaagtatct tgaaggcgga atcatcacga
    16921 gcatcaaatc gctcgaaggc gaccggatca tcaaaatctt ggtggaaaac cacgacgaac
    16981 tcggcgacat cgaggattat ggcatgtaca tggaaattct cgggcgttat gccaatatcg
    17041 ttatcacgga aaaaaatgac atcatcatcg atgcattcaa acatatcagc ccattcgacg
    17101 aagtcggaag aacgatcgag aagggagcaa tatatcttcc gcctttcgat ggcaagacaa
    17161 aaacatctga caaggaagga gtaactgcta ttttcagtta tgccggacag cttaacagac
    17221 gatatctcgt tgacaatatc cgcggcattt caccggtatt tgccgaatat ctgttaagag
    17281 agatgtcaaa gccgggcgtt tccggtttga cattgcttga cgatgtgcaa aaactgccga
    17341 tcaatccggt catgatggaa aaatccggaa aacgggaata ttattatttc gatattttcg
    17401 atattggaca gaaaactttt tttccttctc tatccctcct tttagacgca tatttcaacg
    17461 atctgggaag agaagaaaga aaccgccaaa tgtcgaaata tctgattcaa ttcgtgaaaa
    17521 gagaaaccga caagttggcc aacaaactgg aaaaattgtc ccaagatctt gcgaaagccg
    17581 atgaagcgga catctaccgc atcaaaggtg atttgatatt gcagtgccag aatgaaattg
    17641 ccaaggcatc ttccaatcac gaatgcttct cttacgagct tgaccggaat atcaatatcg
    17701 aattggatcc tttgtcatct gccgtcaaaa acgccaatca atatttcaag aaataccgaa
    17761 aactgaaaaa ttctgtcgcc tatattgaag aacagatcac tatcgccaag gaagaattgg
    17821 aatacttccg acaacttgac gatcaaatcg agaccgcttc attgatcgac ctggaagaaa
    17881 ttcgccagga gttggccact aggggaatgt tacgtcaaaa aacaggcaaa acaagaaaga
    17941 aaaccccgaa ttacgaaacc tatacggaca atgagggaat ggaaattctg gttggaaaaa
    18001 acaatatcca gaacgattac atcacaaacc gcgtagcaag accaaacgaa tggtggttcc
    18061 atgtcaaaaa cagtcacggc gcccatgtca tagcgagaag cgaatccgaa ctgaaggaac
    18121 cccagatcag gactgccgcc aatctcgccg cattgttttc ggaagccaga aattcttcat
    18181 cggttcccgt cgattataca agagtcaaac atttgaaaaa ggttccggga accatcgggt
    18241 ctttcgtgac ttatacaaac caaaaaacga tctttatcga tccggacaat gccgtgatcg
    18301 aacatctgaa aagcaagaaa aaataggttg gaaatccaac ctatttcttt tcgatatctt
    18361 cttttatcag ggaaatgaac tcgtcgactc cgattgtcac ctgatcctgt gagccgtatt
    18421 tgcgatatgt gacttcgtcg cttgacactt ccttgtcgcc gattaccagc tgatagggga
    18481 ttttcttcgt ctgcgcttcc cggatcttgt atccaagctt ttcgtcgcgc agatcggttt
    18541 ctaccctgat gccggcattt tgcatcataa cggcgagttc gttcgccttt tccagatgga
    18601 aattgagatt taccggaatg atcttgacct gtacgggggc cagccaggtc ggaaaagcac
    18661 cggcatagtg ttcgattaga atgccgataa aacgctcgat cgaaccgaac aacgcccggt
    18721 gaagcatgac ggggcgcttt ttctctccgt tttgatcgat ataggtgaga tcaaagcgtt
    18781 ccggcagatt catgtcaagc tgaatggtgc cgcattgcca gatacgattc atggaatcac
    18841 gaagcttgaa atccagtttc ggaccgtaga atgcgccgtc acccggattg actttgtatg
    18901 ggattccctt ggaatcaagg gcgtcggcca aagctttttc cgctctgtcc caggttgcga
    18961 tttcaccgat gtatttgtct tccgggcgag tcgaaagctc gatcgaataa tccagtttga
    19021 agattttgta catataatcg taaagcctta caagttcctg gatttcatga acgatctggt
    19081 cttcggtcat aaagatatgg gcgtcgtcct gggtgaaatt cctaacccgg aaaagcccag
    19141 ccaaagcacc gctagcctca tgacgatgga ccaaacccaa ttcgccgact ttcaagggaa
    19201 aatccttata ggaatgaata tcgcgtttgt aaacgagcat tcctccgggg caattcatcg
    19261 gcttgatgac aaattcctgt tcgtcgattt ttgaaacata catgttctcg cggtaatttg
    19321 cccagtgtcc ggatatctcc cataattcct tgcttagcat gattggggtc tggatgagtt
    19381 tatatccttc ccggagatga actttatacc agaaatcttc gagttcgcgt cttacgatca
    19441 tgccgtttgg aagccagaac ggaaatccgg gaccatactc gctgatcatg aaaagatcaa
    19501 gttcgcggcc aagtttccgg tgatcgcgtt tttttctttc ttccagaagt tccaaatgat
    19561 cctgaagctc cttgtcggtg aaaaacgcgg taccgtatat cctggtaagt tgtttgtttt
    19621 tggagtctcc gcgccaataa gccccggcta agctcagcaa cttgacattt ttcagccatt
    19681 tggtactggg gacatgcggt ccccggcaaa gatcggtgaa gagatcctgg gtatagaccg
    19741 taatctgttc ttcttccggc aagtcgttga tcagttcgac tttgtacgga tctttggcga
    19801 aaagtttcaa agctcccgct ttatcgactt cacgtctgac catcttctga tcgagagaag
    19861 ctagtttttt cattttctcc tcgatcgccg gcaaatccga ttcactcaaa acgacgtctc
    19921 ccaaatcgat gtcgtaataa aaaccttcct cgatcgccgg gcccactccg aagtgggctt
    19981 cgggatatat ctgcctcacc gcctccgcaa gtaaatgggc tgccgagtga cgcaatatat
    20041 ccaaggccag aggattggca gttgtgatga gttcgataga agcatctttt tcaatcgatc
    20101 ttgcaagatc gatttgttcg ccatccactt tgatggcgat gctttgtttc ttgagtgatg
    20161 gcgaaatcgc ttcggcgatc tcgaacccgt tcactttggc tggaaattcc aattcttttt
    20221 tgtctggcaa tttgatttta atcatgataa ttcctccttt ataaataaaa aagtcgtccc
    20281 ttatctaaag gacgacattg atcgcggtac cacctttgtt ccggcaaaat gccggcactc
    20341 gatccgttaa cgcctggtac gggatccttt catggatccg gctcgagggt ggtaaccggt
    20401 tctgtccgtc ggatggtttt caccaatcca tcctctcttc aggcggatat ggaatcgatc
    20461 gtgtcccttt cagggccttt ttcttataac caattataat gcaattttcc catttgtcaa
    20521 gttcttgcgc tgggtttttc cgataattcg aattcaaccg ccaattctct gatcctttcg
    20581 aagatccgga atgccttagt cttctcgatg tcgttctcct ggatctgcat ggcagccatc
    20641 aggaccttca ttttcatgtt cgaggaaaag aacgtgggca attgatccaa cacccggtgt
    20701 tggagaatcg gaccgagaat ttcgtcccta acgaacgaag tctggttttc gccgccgata
    20761 tcgtctagaa acagaaccgg aatttttttc aaattttcga ttttttcttc caaatcccca
    20821 ttcgagatcg ccgatttgag ttccctcaca aggtcgggat aatatgcaaa aatgcttcgg
    20881 tatccctttt tggcgagctc gttggcaata acggcgagaa tataggtttt gcctgtaccg
    20941 aaagctcccg ttagatacaa gcccttcatg ggattctcgg gtgaatattc gttcagaaat
    21001 ttcatgatgt agttatgaac ttccttacgc tctgaaccga taaaatcgaa atctgtgatg
    21061 tcggcgttca aaactttttt cggtaagtac atcatgtcga tcttgttctt gctttcatcg
    21121 aaattgttga aacggcactt ggtgtactca agtgtgattt cattggcgta atagcgcaaa
    21181 atcggtgtca ttccgatggt cgatagcttg cactcgtaga tgccttgaca ttggttgcag
    21241 atgtttttca tgtcgcggta agaaagcaac tcgttgatac cgccaacgat ggtttcgtgt
    21301 tttaagtcgt gtttcagcac aaaagcccgg atgatcggat cttccaggag ttgctcgatg
    21361 gtttcgtccg gctggacgtc ctgggaatag gcgcccagat attgatttat ctttttcatc
    21421 gcgccactca ctccttcatt ttattgattg gatgtattcg tcaagccaat cgattttgat
    21481 atcgtcggcc ggtttggtcc gttgttttct attgccaatg gattccttgg cttgttggta
    21541 tctggaactg atatgctgaa cataggcaat ggcggttttg acatccgtaa cctggttctt
    21601 tttccagtcc agcgccactt tctcgaaata ttcatacccg ggaagcgagc cgtccttgat
    21661 cttggcaaca tatgccaaca agacattaag aacacctctg tcgatgccag tctcttccag
    21721 aattctttcg acaattctta gatcagctgc ggagaatttt cctccgctca tttcggccaa
    21781 aagctgacgt ggagatacga cggtgaaata ttcgacggga tcggaaggaa gattcctttc
    21841 ttccggtttc tcgatttctt tggctccttc cggtatggat gatgccattt gcttgtattc
    21901 ttctctggcg tttacggcca ttttattcac attgatttgc tcgttttcat cgatggattt
    21961 taacaatacg gatcgcatcg tcaactcatc caagccatat acataatgaa gattgagtat
    22021 tttttccttg atttggtttt ggatcttgtt ttcccagtcg gtatcatccg tgagactttc
    22081 gatgaaaaaa cgccaatcga aggttgtgtg gttcgtatgg atcgatttgc ctttgttatt
    22141 gccaagcaga tccgactccg gaagactttc tgtcactccg atcggtcgaa atacttcgtc
    22201 gaacgctttg gtcatgcgag tgtatttctt cagtgctggc ggtttgagtt tgaacgaatt
    22261 caggattttt ttgaatctgt ccttggtgat ggaagcgatc agatactggc cgagaatccc
    22321 gtcgttgatg aaactttccg ccgacaacgg aggttttatt tcatacataa aatgatcgtc
    22381 gctgtaatat gtttccatca gtccgatcgc ttctaaccgg aaacgatcgt tctcgatgat
    22441 gtcaagcctg gtgtcaagca acgattcgag atcgccttgg agataatcgg acgaaacgag
    22501 ggtttgatga tcaacaaggc ctaacaaagt aaagtaaagc gcaaaagcac cggttccgat
    22561 gatcggttgg taaagcaacg agatgatttg gacatcggtt gcacaaagat ggcttttgga
    22621 aaagacggtg aaactgtcgc ttcttctgat tccgtccaaa ggaaacctcc tcagggatgc
    22681 aatgaaacgc ccttgaaatt gagtttatga tcgaaatagg catacatttc catgactttt
    22741 ttcttgggac ggttgaagcc catggaagtg aagaaattga ttttgatatc gatccccgtt
    22801 tcaagcggag tcaactgggt gatggttatg atcgaatcgt atccgcttcc caccatatga
    22861 atttccccgt gagcctgatt gacgttgacg atttccgtcc cggcatcgcc ggaatattct
    22921 tcgattaccg ccagacattg cttgaaactg ttacggtaat agcgtgttct caatccgcta
    22981 tcgatatgca gttcacgtgt ttcgcacgtt gaagagaaaa aactcataaa tcccatctca
    23041 cttcctcatt attgattgga gaattgcttg gaaatcccgc ttggttttgt cgatggatcc
    23101 ggaattgtcg atcacatagt cggcctttcc ttttctattt tctgaggaca tttgggaacc
    23161 tatcttgctt aaagcataat ccttatcgat atgatccctt tccatcagtc tttcgatcct
    23221 gacggattcc aaggcatcga caacgatgac cacatcgcag acacgttcga aaccggattc
    23281 gtataaaaga ggagcgctga cgacaaccaa aggctcgcct ttttcgtcgt aagcatccag
    23341 ttctttgaga atttcctcaa tgaccaccgg atgagacaaa gcattcaatt tttgtcttac
    23401 cgaatcatcg gagtaaattt tatgcgcgag tttctgccaa tcgatcgatt catccgggca
    23461 caaagaaaca tcaggcatgt tcccgactat ttcattatac aatacttcat gcggttttgt
    23521 cagccgtttg tagacaaaat cggcatcaat gatcgggatg ccttcttcct tgaaccatcc
    23581 cgatatcagg gatttgccgg aagcgattcc gcccgtgagt ccgattactt ccatgggcac
    23641 ttgttcagcc atttggcaat gcgggcaaaa ataagtgcct ctcccgccta ctttcgtttt
    23701 ttcaattgtc gttttgcata tgtggcacgg ttcgccttct ctagtatgaa ccatcaattc
    23761 attttgaaat cttccggtga cgccaagact ggaaacataa cttctgatcg ttgatccgcc
    23821 cagttcgatt gctttgtcca aaacagttcg acagttgcaa acgatcgcgt ccatttctcg
    23881 catagacagg tttccggccg gccttttcgg ataaatccga gatcggaaca aaacttcgtc
    23941 aacatagata ttaccaagtc cggaaacaac cgtttggtcc agaagcgcgc ttttgatcgg
    24001 tgttttcgaa ttatgcagtt tttctctcag atatgcggaa gtgaaagctt ctgcaaaagg
    24061 ttccaatcca agcttttcca ctccgtcagc catgatttcc cgaccaaacc ccaccagttg
    24121 catagtaccg aacttgcgca tatcatcata acgcatggtc gtcttgtcgg tgaaatgaaa
    24181 gatgatatgt tcatgttttt cgataggcga cgactccggt ttaagaaaga atttaccttc
    24241 catccgaaga tgagtgacta gggtatagtc atccaagtcg aaaaacaggt attttccctt
    24301 gcgccggata ttgcgaaagc tttgtcccgt cagcttgctg gcaaattcat ccgatgaaag
    24361 atgtttgatt attttatcgt atcttgtttc gacagaagcg atcgttttcc ctagtatctg
    24421 tcttttcagg gtttccttga tcgtttccac ttccggcaat tccggcatat catttcacct
    24481 cgtaaagatt atttccgaaa ctgctttcga cttccagcgg aaccgagagc tccactgaat
    24541 gttccatcag tctgataatg agatccaaag ctttttcctt ttctttttcc ggaacggaaa
    24601 aaacgacttc gtcgtggatc tgcagaagca ttttagtttt cagatcccgt tctttgatgg
    24661 ctttgcccat attgaccatg gcgattttca gaatatcggc cgcgcttccc tggatcggag
    24721 cgttcattgc catccgtttt ccgaattcgc gggtttgata attgttggag gcgatttcgg
    24781 gaatgtagcg ccggcgatgg aacaaggttt ccacgtatcc gtttttcacg gctccttcga
    24841 ttgtttgttc catgaatcct tttatttccg gaaaactgtc aaaataatcg gcgatgaatt
    24901 tttccgcctg tttgggactt attttcaaat cctccgacaa accccaggcg gtcttgccgt
    24961 agatgatgcc gaaattgaca gctttggcct gtcttctttc cccggacgat acttcggaat
    25021 tcttgaaaat caatttggcc gtatgggaat ggacatcctc tttttcccta aacgcctctt
    25081 tcaaaccttc gacatccgcc atttccgcca aaacccgaag ttcgatctgg gaatagtcgc
    25141 aggataaaag ctttgtaccc ggatcggcga tgaagacttt gcgtagttct ttcccttcat
    25201 cggtttttac cgggatgttt tgcagattcg gttcgacaga tgataatctt ccggtctggg
    25261 tcaatgcctg tttgtatatc gtatggatgc ggccgtcttc tttgagacgc aaagcggact
    25321 taagtccttc ataatatgtg gaataaagtt tggtaagtgt ccggtaggaa atgatccggt
    25381 cgataatcgg atgaaaatca ttcagttcct tcagaacaga tatgtcggta gaatatccgg
    25441 ttttgttctt tttcctatac ggcaacccga gtttctcgaa caagatagct ccgagctgct
    25501 tggggctgtt gatattgaag ttttcaccgg caagttcata gatggatgtc tctagttcgt
    25561 caattctttg tttcagctga aatccgaaat catccagttt tttttcatcg acggcgattc
    25621 cttctttttc catgccagca agaatgcttg cgagcggaat ctctatattg ctaagtaatt
    25681 cctcttgtcc gcattctttg ttcatgataa gcattttttc gtgcaaggca aagattgcgg
    25741 cggccttgtc aatggaatgt ttggcgatga cattgtcttc gggaagggca tagctcgctc
    25801 ccttgccata gacctcttcg tcataagcca cgtcaagata atcgaaattg gatacgactt
    25861 cccggaatcc ttcttttgtc atattgggat tcaaaagata tgcggaaagc atcagatcga
    25921 acgaaactcc ttcaagatca tatccgtccc aaagcaatgc cactttggtt tgtttcaagt
    25981 catagacaaa cttttcctta tggctatcca taagccagtt ctgcaaatcg ggggataaag
    26041 gcaaaagaga atagggaatg taataggatc ctttggcacc aacgagggca aatcccagtt
    26101 tttgcgcgat atggtaattg gaaccgaaga tttccagatg gagtgctacc ggcgaagtaa
    26161 gaatcgaagc gatttcctcc ggacggccaa taaccttgaa accggaaaca tcctcttttt
    26221 gtttggctgt atctagttta cgcaaaaagg aatgaaatcc catcttttgg taaaaccgct
    26281 gaagcatttc ctcattcggt ccgtggtatt caagtttgtc tattgatatg tcattggcga
    26341 aatcggtgac gattgtcgcc aaacgtttgg agaattccgc ttgtgcggca ttgttctcga
    26401 tttgctcttt cgtctttcct ttgagagaac cgacattcgc caacacgttt tccaacgttt
    26461 tgtattcgtc aagcagtttg gcggccgtct tcggaccgat accgggaaca ccgggaatgt
    26521 tgtcggacgg atcgccgacc aaacctttgt aatccgtcat ttggtccggt ctgattccca
    26581 tctctgcttc cattacggaa gaagtgacaa cgtcatagtc gctgaatcct tttttggaga
    26641 tcaattgact gactttttcg tctagcaatt gcataaggtc acgatcattg cttattagta
    26701 aaatctgatc gaattctggc gcaaaatgtt tagcggaaaa cccgattatg tcatcggctt
    26761 cgtatttttc tttctcgaaa taaggaattc cggcagcgct gagaaattct cgcatcagag
    26821 gaaattgcat aaccagttcc gacggagtct ccttgcgggt tcctttgtat tccggaaaaa
    26881 gatcgtgtcg gaatgttttt cctttggcat ctagcgccac catgacatgg gtgaaattca
    26941 ttttgaatat ctgttggaag gcgcggacaa aaccatagat tccgtttgta tagactccat
    27001 cctggttttg catcaaattg cctgttgcgg cagttgcgta atacgatctg aacataatgt
    27061 tgtttccatc tatcagcaac agttttttca ttgttcaccc cgccttttgc caatatttta
    27121 acataatgag gtcaaaaaag cacatgagca tgtgcttttc ggtttttcgg tctattttgt
    27181 cgcattcatt ccggccacca gcaataagcc tttgagaatc aagtcctcgt ccaaagtcat
    27241 tttcgcttgg cattcgggat atatgatctt ggcgttgccg ccggtaagaa caaccgcgag
    27301 atccggattg ccagtttgtt tcctaactct tgcgacaagc ccgtcgacca tcgatgcatg
    27361 gccgtaaata agacctgatt tgatcgaatc aggagaattg gttcccagca gtttgcttgg
    27421 tttctccaaa ttgatatgcg gtaagagtga agctttggaa atcagggcgt tcaggcttgt
    27481 ggcgagaccc ggagcgatga tgactccctt gatgccggaa ttttcgatat aagtcatcgt
    27541 tgtggcggtt cccatgtcga taatcaggca tgtatgactg taaatttgcg atgcggcgac
    27601 gcaatcggcg attatgtcgg ctccgacctc tttcggggta tcggtgaaaa tccggattcc
    27661 ggtcttgacg ccgcttgcca gaaatatcgg ttcgaatccg aatcgggaag aaaaataatc
    27721 gcgaaacact tcattaagtt ccggaacaac cgacgctact atcgcctgat cggcattttc
    27781 aatcaattca tccagaatga tcgcatattc atcggatgac cgggatttcc cggtactgat
    27841 ccggaatttc ttgactatcg ttttcccgtc ggtagacaaa ccgatggcaa tcgtcgagtt
    27901 accgatgtca atcagtacta gcattgccgg atcattcctt ttcttcggtc tgtaacgatt
    27961 tgtttctgac tagagattct ttgagcctcg ttgtcacaga agcgccgacc agaccggcca
    28021 gaatcgcttc ggccggagcc gaaatcggaa tgccccataa gacaagggct tcaagatagg
    28081 gaatgaacgc ctcttttaac gtgaaatgat tgacgagggc gaatgaagtc caaaacatgg
    28141 aaagcaccaa tatggtatgg atgagcgtga gaattaagaa actgacccca taggccaaat
    28201 gacggttttt gatgaaccgt tggaagaatt ttaccacatc gaagattatc aatccgaata
    28261 ttatccgggg cagaacagaa acccagggat agatgaaaaa cggactgctg tagataaaag
    28321 cggcgatcat cgacacaatg ccgaaggccg taccgagcca gattcccatc tttctaccga
    28381 ataaagcggc tccgatcaat accggaatat gaatgatcgt cgcttccaaa tcaggagcta
    28441 tcttgatgaa tccgatcgtg gatgcccccc agccggacgg aaccaggccg agaagagcga
    28501 tgatggcgat gaacatcgcc atcatggtaa gttcgcgggt tttctcgcga ttgcttttat
    28561 tgcttttttc gtttgtcatt ttcattatcc tttccaagcc gcattagacg gtctcataag
    28621 aatcatgctt atttgatttt tatacgtttt tctttatttg ttctatcatg ccgtgatcaa
    28681 gtttttttat catggccatg atcacttttt tcactgcttc gtaatcatct ttatgaatca
    28741 ttgaagcaga cgaatggata tatctcgaag gcatgccgat tgtgcaaacg agcacgccgt
    28801 cgcgggacaa ttgtacctcg gctgcgtcgg ttcctcccag tgatttgtaa tactggaaag
    28861 gaatattgtt ttctttggcg gtttcttcga cgaacattct cacaccacgg tgcatgatac
    28921 agcgcggatc gtagaaacgg atcataaatc cgcctcccag cttgcctccg gtttccgttt
    28981 cctcgaaagt atcggaacat ggggaacaat ccacggcgat gaaaaagtcc ggtttgatca
    29041 tgtaagatga tgtcttcgct ccgcgaagac caacttcttc ttgcacgtta gcgccgcaat
    29101 ataaccggca aggaatattc tctttctcaa ccgacgacat tatgtcaagc gccatcgcgg
    29161 atccgaaacg gtcgtcccag gctttcgaga tgattttttt cttatcgaat gtctcacggt
    29221 aaatattctc aaatacgatt tgttgtccga ctttgatgcc catttcttcg gcatgcgctt
    29281 tcgaatcggc gccgatatcc aaaagaaaat cgtcgaacga ggtcacctgt ttgctcttgt
    29341 cgtcccgtaa aagatgtggc ggtttggctc cgacaacgcc gggaacggat gaaccgtcat
    29401 ccatgatgat attcatgttc tggctaagca tgattggcgg attgatcgaa ccgatcggaa
    29461 tcattttgat gaatcccttg tcggtgatgg atgccaccat cgcgccgact tcatccatat
    29521 gaccggctat catgacggtg ggtccgtcct tggtttcgtt gacagatccg aaaatcgatc
    29581 ccagtttgtc ctgatagatg ttttcggcca catcttggta tttttccttc agataattgc
    29641 ggatgaaacg ttcattcccg gaaatgccgg gaagattgac aagttccttg tagaattgct
    29701 tcatttccga ctcctttcga cgcttgcttc aagataaaag attggtaagc ccatctttcg
    29761 atatttgatt tcaaattctg tttccacgta tccgtccgat tcaatgcctt ttcccagagc
    29821 gatatcggtt atggaaaaat catcgcttcg gttgatgctc atcatactgt attcgaacat
    29881 cggaaaattg tctgtgcgca aatagacttt gccgttttca gagagtatct gacgatattt
    29941 cggtagaaac gcttcgtttg tcaaccggta tttggctttt ctggtctttg gccagggatc
    30001 gggaaaatta atgtagatat gtttaatttc atccgaatcg aaaaacaaat cgacatcggc
    30061 cgcatccgcc aatacgaata aaagatttgg aagcggatcg atgatgagtt tttctaacgc
    30121 cctgactaaa acggaatcga atttttcgat accgatcaaa ctgacatccg gatttttccg
    30181 ggccatttcc atcaggaaaa gacccttccc gcaccctatt tccagggata tcgggttatt
    30241 gtttccgaaa gttttgttcc ataaaccctt tttgctttcc ggataggcaa tcacatattt
    30301 cggattgttt ctgatagtct cgccggcatg tttgactttc cttagtcgca ttattcgcca
    30361 aatcctgcca gtttttcgat agcttccgcg taaattgccg cggtaactag caatttatcg
    30421 atttccagat attcgtcagg ctgatgagca agatcgggat cgccagggaa ggcggcacca
    30481 aacgcgaccg cttttttcaa caccctagca taagttcctc cgccgatcgt catcatcggg
    30541 gaaacgaagt cattcgtgta tttctggtat gctttataca atgtctgcac aaacggatcg
    30601 tcaggtgaaa caaaatggct tggggaattg ctttccgtct cgaatttgag tccgtatttc
    30661 gtcgcttttt ctgtgatttt cacgactccg gcctcaaaat cataattgcg gggataacgg
    30721 atattgcatc cgacatagaa attgttgttt tccattctga caagcgctgt gttgattgtc
    30781 aattccttca tcagttcgtc ataacagtca attcccagtt tttcaccggt atggtcgaac
    30841 aaaagacaat tgtcgatgaa tctgatcaat ggatggtttg tatgttcacg tagaaattca
    30901 gtcattaaaa agacggcgtt gactccgtgt tgcggccacg cagcgtggct gctctgccca
    30961 taaatcacat attttttccc gtctgtctct ccttgatatc ctttcaattg caagaaggcg
    31021 gtaaattcgg ttgaaaggtc ttttttaaga attgctacgc attcatcagg aacgatgttg
    31081 taacgttctc cggcacgcat cgattcaagc aaatcgtcgt cgccgtagcc aagaatgtcg
    31141 aaactgaaga ttcccttttc tccatagatc aaaggaaaat cagcgtctgg agcaaatccg
    31201 atatccggca tggtttcttt ttcgatatat ctttttacgc cacgcattcc cgattcttcg
    31261 tcacatccga aaatcagacg gattcttttt ttcaaaggaa tattggcgtc atcgatcatt
    31321 ttcatcgcaa tcagcgcggc taccgaagga cctttgtcat ccatcgaacc acgggcatag
    31381 atctttccgt ctttgacagt cgccgaaaac ggtggatatg tccactttcc gcctgccggg
    31441 acaacatcaa gatggccaag gatcccgatc tgttcttctc cctcgccttg ttcgatatac
    31501 ccggcgtaat gatcgatatc cttggttttg aaaccgtact cttttccttt ggccagaaca
    31561 aagtccaaag ctttgcgaat cggttctccg aaaggatatt ccttgtcttc tggatcgtat
    31621 ttctccaaga ccgtcggaat ctgtaacaaa ggcactatta catccaaaaa ttccttttcg
    31681 tatttttttg cctgctttag ccaatccatt gtattcacat ccttgtatta tcctttataa
    31741 ttgtatcaca tcattcattt ttttcaaagt agtagaaagt gtcaaacctt ttcattatct
    31801 attatttagg ttttgtttga caaaacacaa tttataagta taatgtatat atattatata
    31861 atatatcata ataatgtgag aatctggcca ttattttagt atagcaggta ctgatgatga
    31921 tacgtaaaat tataatgaaa aacttgacct gttcgaactg cattgcgaag gttgaaagaa
    31981 gaactgcaag gttgccatat gtcaacagtg cgagttttaa ctacgccaat cagacccttc
    32041 tcgtcgattt caagaatgat tacgaccaag atgtagcgtt aaaggaaatt aaaggaattg
    32101 ttgatgtttt agaagataac atcgatacat attattacga acaaaaacca atggaaaaga
    32161 aaagcaattt catccgagaa tacattttgg tattgatcgg aatgttgatc atcttgctga
    32221 ccttttcctt ttatcatatt cccgaatatg tgaacaaaat attcggaacc aatctgcatg
    32281 agtatttggg aaacgaagcg gaactcagat ggttaagaat aaccttgtat tgggtcggat
    32341 atgttttatt aatttcgaga cttttgtatt cccaagtcaa aagcatccgc aatttcaatt
    32401 tctttgatga aaatacgttg atgatcattg cgactttggc ggctatgttg atcggaaagc
    32461 acgaggaaag catcggcgtc gtcattttgt attcaatcgg agaatatctt caagcgcgag
    32521 cggtcagaag atcaaaaagt gagattgctt ctcttatcaa tctaaaaatc gaatatgcca
    32581 atcttctgat aggcgattcg attgtcatca aagatccctc ccagatcaaa gttggcgaca
    32641 cgatcgttat caaaaacggc gagcgggttc cggtagatgg aatcattgtc atgggatcca
    32701 ccagccttaa caccagcgaa ctcaccggcg aagcaaaact tcgcagcgtc gatgtcggca
    32761 gcaaaattct ttccggaaat atcaatgtcg gcaacgtgat ccatgtccga gctaccaaag
    32821 aatacaaaga ttccaccttg gccaaaatca tcgatctgat cgaaaactca accacaaaca
    32881 agtcaaaaac cgagctgttc ataaccaaat tcgcgaaaat atacactccg ataatcgtcg
    32941 gtatggccgg tcttctcatt ctgttcggag ctatcttcga caatgccaac ctgatgacgg
    33001 aaggcggata tgtatacagg gccgcaattt tccttgtgat atcatgtcct tgttcgcttg
    33061 tcctttcggt gccgctttcg tattacgccg gcatcggcac ggccgccaag aacggaattc
    33121 tgttcaaagg ctccgccttc ttgcagaaaa tcaccgatgt ccagacaatt gccctcgata
    33181 agacaggaac cctgacctat ggcgacttct tcgttcagga agccacatcg gacgatgttt
    33241 tacagaagat cgcctcaatc gaacaatatt ccactcatcc gatcgcctta tcgattgtca
    33301 aatacaacaa gttgccgcta tccgaagttc aaagtgtcga ggaagttccc ggatacggaa
    33361 tgaaaggcat cttggacggg aaagtgatcc ttgccgggtc taagaaattt ctcaccaata
    33421 acaacatctc agtcaccgat aaagtgatgt cggtcggctc cctgactttt ttggcaatcg
    33481 atggcaaata tctcggatat gtcatcgtca aggatgagct caaggaatct tccgtcgata
    33541 ccgtacgcaa gttcacaaaa cacttcgata cggtgatgct gacgggagac aatgaagaat
    33601 ctgccggtga aatcgccagt aaacttggcg gaatcgaata ttacgccgat ctgttaccgg
    33661 accaaaagtt ggataaattc aacgaaatcc gcaccaattc ggtctctatg tacgtgggag
    33721 acggaatcaa cgacgctccg ttattgaaaa acgccgatat cggggtatcg atgggttccg
    33781 caagcgatct cgccatcgaa gtttccgatg tcataatcat tgacaacgat atcagattgt
    33841 tggaaaaagc catcaagatc ggacgcaaaa ccagaactat caccatcgag aatattgttt
    33901 tctccctagt tgtaaaaatc gctttcctga tcttatccgc tgtcggatac atgtggatgt
    33961 ggctttcgct tttcgccgat gtcggcgtgg ccttgctttg cgtaatcaat gccatcagga
    34021 tcatatacat gaaaagatat ttcgaagatt aaaaacggaa accccgaagg tttccgcttt
    34081 ttttataagt gtttgacgat ggcgtaagtc ttcttgcctt ttcgtaaaac ggtatacatc
    34141 tttccgatag cttggttgga atctgccata taataagtgt cggtgacttt ttcaccgttt
    34201 attgtgatgg ctcccgatgt gacaagttcc cgtgcctgac gcttggaaga ggccaattgg
    34261 cagttaacca aaacatcatc aattgtttcc gctttatcag tctcgtattt tatcaggttt
    34321 ttgaacccga tgccgatctc ctcggcgtcc agagtgtgga ttttaccggt gaaaagagca
    34381 tcagtgatcc tttctgcgcg gaggcactcg ttttcgccat ggacgagtgt tgtgatctgg
    34441 cgggcgagtt ctttctgggc catacgcgtt tccggttcca tttttacaga caattcgatt
    34501 ttttctatct cgtccttgga gaggaaagtg tattttttca gatagtttat ggcatcgtcg
    34561 tcggcagtgt tgatccagaa ctggtaaaac tcgtatggag atgtcttttc tttgtccagc
    34621 cataaagtgc cggattcggt cttgccaaat tttgttccgt ctgatttcgt gacaagcggg
    34681 aaagtgaaac catatgcttc gctgtcggct ccgtggagct ttctgatcag ttcgagaccg
    34741 gatgtgatgt tgccccactg gtcctgcccg ccgatctgga gcttgcagcc ataactagca
    34801 tacaaatgct cgaaatccca agcttggatg atctgatagg caaattccgt gaaggaaagc
    34861 ccgttttcga tccgcgatct gaccgattcc ttgttaatca tgtacgatat gttgaaattc
    34921 ttaccaatat cgcgtagaaa ctcgatcgta ttcagggatg atatccagtc gtaattgttt
    34981 acgacctggg cgtcaggtag caacctgatg atttgttttc tgattccttc ggcgttatcc
    35041 aaggatgttt ggatggaaag caactgcctt tcctggtttt tgaaactcgg gtcgccgatc
    35101 aatccggtgc caccgcccaa aacaatgatc ggatgatgtc cgtggttttc caggcgtttg
    35161 gcaacgagac atgccaaaag atgtccgata tgcaaactgt ctgctgtcgg gtcgattccg
    35221 atataaaatg tcaatttttt ttcgtcgaga attttttcta gattcggatc cgtctgatca
    35281 taagccagat cccgccattt caaatcacta aataagctca ttttttcaca acctcaccta
    35341 ttaatatttt ctatcaaaaa ataaaaatcg ccctttttac aaaggacgat cgattagacc
    35401 gcggtaccac cttaattcac cattggtgcg ctcgtaagat aacggattac ccgtctgttc
    35461 acacagaaac tcataagtgt atttcggaat ggacctgcct tcggttcgca ccaaccaccg
    35521 aatctctgaa gctgccggtt tcatgcctac taagcttatt cttcgttata tttggttgtt
    35581 tgtctttcca tgatgctgtg gggcaatacg ttgaatttga cttcaaccgg ctccccgtcg
    35641 atttcctttt taagcaaagc catggctttg gctccgatat cgataatcgg aatatcgaca
    35701 catgacagtt tgggacgggt cagggaagcg tatttggtat tttggaagcc ataaacggaa
    35761 atgtcttccg gaactctctt ctttctttcg atgatcgagt tgacaaacga aacggcgatc
    35821 gaatcacgga ccgcgatggc tccgtcaatc aacgtcgcgg gatttttgaa gagttcggcg
    35881 aaatgcacgg tattgatcgt tatgtcgccg ctcgtcctca caactttcgg ttgtaatccg
    35941 gcatctttga ttgctttcag atagccttct tctttgaggt cgttaaccat atattttctg
    36001 gctgttgtca ataagtagat gtttttccgt ccttcgttga cgagacgcat cgtcagttcg
    36061 tatgacgctt tgaaataatc gatacagaca gtgacgagtt tgttgtcata cggatacagg
    36121 gtattagcca aaacaatcgg aactttgtaa tcgtcctgaa tcgaattcag gagagaatac
    36181 tgttcctcgt tgatttcatc gttcaaatag agaattccgt ccacctggct ggcaaccact
    36241 tcctgcaaag cttccggttc ctcgatttta tcatccttga gaacatataa aagcagggaa
    36301 taattatatt tgcgcgcttc gactgcgatg ccgttaagca tctcggcaac cgatgccctg
    36361 gacatgtcgg gaacgatcac accgacggtc gtgctcttct tgctggcaag tcctttggca
    36421 aaagcgttcg gcttgtatcc gagttctttg attacgcgca aaaccctttc tctggtctcc
    36481 ggttttactt tttggggatt gttcagcgtc cttgatacgg tagccaagga cacattggcc
    36541 gcataagcaa cattgtaaat tgtggctttg ttcatgttat cgccccgatt ctggctattt
    36601 tccttttttt attttaccat ttatgaaagc gtctttcaat ggctttaaaa caaaatgtaa
    36661 aaattttcat aaagttagcc ttatacgaaa aaaagcaacc ggtaggctgc ttttttctga
    36721 ggtttttagc gtttttcttt gattctggac gccttggcgg aaagtccgcg gatatagccg
    36781 agatgcgctc tcctgacttt tccctgacga actacctcga tcttgtcgat aaccggcgaa
    36841 tgcaatggga atgtacgttc gacaccgata ccgtaggaaa ttttccttac tgtgaaagtt
    36901 tcgctgactc cgccgccttg ctttttgatg cataggcctt cgaagacttg gattctttca
    36961 cggtttcctt ccttgatttt gaccgacact ttcaaagtgt ctccgggacg gaacgacggg
    37021 atgtcatcgc gaatgaattc gcttgttacc atgttgatga tctgctgcga cataataggc
    37081 caactccttt tctcttgtcc aagttcatgg cctgttgcca gcggaactaa gagcatgtga
    37141 ttctcattct tacagcgaaa atcattataa cataactcgc ttggtaaatc aatctttttt
    37201 gtttggcttt ttgacgagat cgggccgtct ttcaagcgtt ctcttcaagg cttgttcgcg
    37261 acgccatttc tcgatttcag cgtggtttcc gcttacgagg acttccggca caaactggcc
    37321 catgaagtcg tatggtctcg tatattgcgg atactccagt aacccggtga aaaacgaatc
    37381 gtcgcggtat gattcttctt ttcccaaagc gcccggaaga agtcttacga tgctatcgat
    37441 taaaatcatc gccggcactt ctcctccggt caggacataa tctcccaccg atatttccat
    37501 atcgacaaga tcgcggattc tttcatcaaa tccttcgtaa tgtccgcata tgagaataag
    37561 atgttcgtaa ctggacagtt ctattgcttt ggtctgattg tatgtttctc cttgcggtgt
    37621 cagcaatatc ttcaatgctt tctcatagcc gtcaatattg acaagtgccc ggtatatcgg
    37681 ttcgaccgaa agcaccatgc cggctccgcc gccgtagggg ctgtcgtcaa cctgcatatg
    37741 tttgttggtg gaaaaagccc ggaagttgat cagttcgata tcgacgattc cggctttttg
    37801 cgctcttccg atgatggatt cagaaagaaa acaaccaaag atatcaggaa acaaagtcaa
    37861 gacggagatt ttcataagag cccctccatg tcgacgattt cgattatctt ggcattcaaa
    37921 tcaactttga tgacaaactc atccctgaat ggaaccaaag atatcttgcc ttcacttcct
    37981 gttatttcaa gatagtctcc ctggggcaat tccctgattc ctttgacatt ccctttttta
    38041 gttccatttt gcatgacaag aagacctata atgtcgtcag cgtgaaattc gttctcaccg
    38101 agagctagat cgtcttcgtc ttcgacaaag atatccgatc ccttgaatga ctccacgtcg
    38161 tttatgtcgt catatccttc gagtttcaat aagagcatgt ttttaaataa gcgattactt
    38221 tcgaccttga ctgaaaaatc ttcttttcct tgacggatca aaagcgtttt tccgaaagca
    38281 aaacgatttt ccgcaaagtc agtcatgaca accactttga gttcgccttt caggccccgg
    38341 gtattgacga tttttcctat tttgatcaaa tccaaaagac attctccttc ttcgggaaga
    38401 ataattttat cccctgactg ttatctcgcc ggtttttctt aatattgttt tggtcacaac
    38461 cggtccggat agttcatata cgagagtggc agccagcact atggcacgga tctgggaacc
    38521 catatcggca tccaagatct gcgtggctac caaactgaga ccgatggcta ccccggcctg
    38581 cggtaacagg gcaaagccaa gataattggc tattttaggc tctgctttgg aaattctggc
    38641 tccgaaccag gctccaaaga acttcccaaa aacccgcatc agagtataga caattccgat
    38701 cacgccgacg acaagcaaca ccgacaattt cagttcggct ccgcttaata cgaagaacat
    38761 gatgaaaatc ggcggggtga tgaaatacac caaattgttg acctcgtcgt atttcttgct
    38821 gaagttggca aatattgctc cgagagccat acaagccaaa atggttgatc cgttcaccag
    38881 atccgccaga tagatcgtca cgaagatcat ggtcacgacc aggctgatgc gattgccgcg
    38941 tccggtaaac cacagacatg cgtatgtaag caagaacccc atggcggctc cgattcctaa
    39001 cgaaaagaaa atttccttga gcggattcaa aatctgaatg atcagggaag ccccggctgt
    39061 ggacggtccg atggcatttg ccacggcaac gcagataccg aacaacatta ccgccaccga
    39121 atcatcgaga gcgacaacgc ccaaaatggt gtcggtaagc gacccctttg ctttatattg
    39181 tttcaccacc atcagggtgg ccgccggatc ggtcgcagcc gcaacagccg acaggatcag
    39241 ggaaaaacgg atgctggtga tgtcaagttg cttggtgatg gcgaaataca agatcagacc
    39301 ggcaaaaaca aacaagacac cgaaagaagc ctccatgatg gcgatgataa tcggtttggc
    39361 tccgactttc ttgaaataag atactttgaa ttcatcgccg atagtaaagg cgatgaatcc
    39421 caaagccacc atcgacacca aatcaaggct ttcggtatag gattccttta ttatattcaa
    39481 tagactcgga ccgatcaaaa gaccgccgat caaataaccg gtcacattag gcaaatgggc
    39541 tattttagcc aatttgccga acaaaagacc ggtcgcaatc attatgcccg cataaaataa
    39601 tatcgtcaaa tccataattt catatcacct agcacttata ccctttgaca ttttcgatcg
    39661 gaaccgtgaa agcaatacct gtgttgggtt gactgaggtc gccgatggaa tcgtttatca
    39721 cctgataaac ggtttccacc tgtccgtctt cgacaaccat catgattact tccgattcaa
    39781 cacgcggatt gtcaaacagg gctttcagag aaccgataaa tttcatatca tcgttttcga
    39841 tgagttttct tgccattccc gaactgctga taatggtagc cccatggatg ccgttttcct
    39901 tgagactgtg aagaaactcg tccaacttgg tgacatcatt caggacaaaa aagaccagct
    39961 tcatatattt ctacctctct ttacataccg gtttgtatta tacaccccag agggcgaaaa
    40021 ggcaaatgct atctcacttg gaaaatggga aaaaagctgc taaaaaagtt ggaaatctga
    40081 attgccaaaa cagttcagta tgagtagctt ttttgtcaat tacatgaatt agcctgtctt
    40141 catggatttt cttgtttttc aataactttt gcagtttttt cgtatctctc aaaaccgtaa
    40201 atgcggcaaa cggcgtctct tttgcgccca tatcatgata tatgcaggta tctggcggta
    40261 aatcggtttt ttccaacaaa cggaaaagag atttgcctac gacatggatg gcgggagaaa
    40321 atatccctgc ttttccgtac acatctggat aacggcacag catgtataaa gatatgttgc
    40381 ctccacatga actacctgcc acacctgtat tcaaacgatc cggaagggta cggtaatgcg
    40441 aatcaacaaa cggtttgaca atctttgtca cgaagccggc ataaatatct ccttccggtt
    40501 cgtttttttc ctccggcatt tccttatcta ggcgttttct catccatggc gacaaactgt
    40561 cagaatattc caggatccgc attttcgggt cattgtcaat tcccactatg attatccttc
    40621 tcgccagttt gttattgaaa agtctttccg ctgtttggca aacatcccag gaataaccac
    40681 taaaaggggc cggatcgaca agattctgac cgtcatgcat ataaagaacc ggatagcgtt
    40741 cttcggaata atcgtaatct actggcaaaa aaacccggat tctcttattc ctgccaccca
    40801 gttcttttat ctcgaagtca tggatcatcg tgttttgctt cgtgtccata tccgattatg
    40861 cgaacaagaa ggccagagcg agaagaatct gagcagaata atatgtcatc agattcaaag
    40921 cgacaaaact tttcggatga tcttttttaa aatagatttc cgaaagaatc aggtcggaaa
    40981 taccaaacag aatcgccccg atcatgataa taacggaaaa agtattgaaa ttattttgaa
    41041 cggcattggc gaatgattgt ccgatcatta ggaaaatcat gaacgaatag gcaatagtgg
    41101 gaaaaagcac tttgccgaaa ttgaatttaa gaacctttat ggccacaaag aaaattgcgg
    41161 ttgtcataat ggcggtgaaa acaaacggat aaaccgacca gtccgacaag tgtaacagag
    41221 caaaatagta aaaaacatgt ccggatgcaa aagcgatggt tcccgaaaga atgatgcttt
    41281 cattttcttc tcgcggacga agcggtctca aagcaagcaa tatgtctccg atcaatccgg
    41341 cgatcaaagc caaagtgaaa agaattgcgg ccggttggaa aacaataaaa tcagctccaa
    41401 ggtaatgggg ggattctacc cacaccatct tttcatagat tacgacggca aacagaaaaa
    41461 taaaacaaaa gcttgccagc gttttgctaa aaagcgcgat tagcggatta ttgtggtact
    41521 cggaataaat ataaagtccg gccacaacaa cggctacgcc aagcaaaaac caaatcattt
    41581 gattttcccc cttcatttaa ggtgatttca ttataattat acattgtatg aaggacattt
    41641 tataggtcca aacccaaaaa agaatatctt ttcgcatatt attcggatat aataggggta
    41701 agtgaatggg gggagtctag tgaaaaaagt cttaaaaatc tttctgattc tggtatgtgc
    41761 gatactggta tttggaatcg gatttattct taccctgcaa atcttcgaat atcgacccga
    41821 tgacataaca attcttgcca tcgacaacga accggaaaat tccgtcacca attatgtggg
    41881 tattgatgaa accgtcagta taatgacgtt caataccgga tacgcatcct tatccgccac
    41941 cgaagacttc gtgatggacg gtggagtaaa aggacgcatg gattccatcg aggaagtcga
    42001 agtcaatatc gccggtatca aaagtatcat aaccgccgcc gaagccgata tctatctgct
    42061 tcaggaggtc gacacagatt cggatcgatc atacaacaca atgcaatatg acgagtattc
    42121 gaccttgctt tcatattccg gatcgcttgg atataactac cgatgcattt tcgtcccgtt
    42181 ccccttcaaa atgggtcaaa tgatgggttc ggtcgattcc ggaatcgcca ctttcagccg
    42241 attttataca agtgacgcca caagatacca gttacctgga agtttctcct ggcccgtgag
    42301 tcttgcgaat ctcaaacgtt gtgtcgttgt atcacatctg ccaatatcgg gatccgacaa
    42361 atatcttaca atcgtcaatg ttcatttgtc ggcttatgat gacggcacca tgagaatcca
    42421 ggaaatggaa gcattgcaaa atatagtcga agctgaatat gaagccggta actatgtgct
    42481 tgttggcgga gatttcaacc aaacattccc ggacgcagtc acaattactg aaaacattga
    42541 cggaaccgaa acatacgatt acttatatcc gctcaaggaa gccggccttt gggaagcata
    42601 tccgatgagc gacacctggt ttaccgataa cgggtttaca tttgtcaatg atatttccac
    42661 tccgacatgc cggttgttga accaacctta cgattcggaa aatcatgaca acaatcaata
    42721 ttattcgatc gatggattta tcgtatcagc caatatcacg gtcgaatcaa tacaggtaat
    42781 cgatcaggat ttcatgtata gcgatcacaa cccggttttg atcgagatca gtttcatcca
    42841 ttaattttac attaaaacaa taaaaaaagc atccggctta ttcggatgct tttcttttat
    42901 tcatgatttc cgtgatttcc gtcttcaccg tgatgatgct cgtgttggcg acatatgata
    42961 tcgctcgata caagttcgcc ggccaaatat ttggcaatca catcggatac ttcgccgttt
    43021 atccccataa tcacctggat accgaagcca tccatgattt tctttgccat ttctcccatg
    43081 tttccggcaa tcacacagtt aacgccgttt ttgtgaagaa agttgggcaa gaaccctttt
    43141 tgatgcggcg gattcttgat aatctccgaa ccttttgctt ttccgtcctc tatttcatag
    43201 acaacgaaat aatcacagtg accgaaatgc tctgtcacca tattatcggt taccgccaaa
    43261 gccactttca ttggattttc ctcctccgtt catatgtctg caactagagt tgtttgaatg
    43321 ccgcggtctc acacaggaat tgcaatcctt ttcgcaaaat acataatgcc cgccttcgat
    43381 gaccaaattt tgtccggata cgagcgcatt ggcgattttc tttctcgctt catcataaat
    43441 acgttgaacc gtggttctgg caacattcat gagttttgct gcttcttcct ggtccaatcc
    43501 ttcttgatca agcagtctaa tcgtttcgta ctcatctaga ttcaaaacaa tagtctccga
    43561 tttatcagag ttacgatcca acggaccgta tttctgaaac tctggcattt tgcatatgca
    43621 acgcggttta tgcggtcttg gcatccaaat caccttccct tattacacgg gaattatagc
    43681 atatatatga acatatgtca ataattatgc cggattttcc tgtggcgtct gttctttttc
    43741 cttgcgttcc tcaatttctt tatttatcat ttcgtaatat ttttcgtcaa tgaaatattt
    43801 ttttctcaga atatagaatg atcctgtaat tagaatgatc gggataatcg taatggaaat
    43861 ccttagcaac aaacgcattg ttccggtcgt ctgatccttt aatacggaat cggctgccgt
    43921 attgatgacc atcgaataca ttccggtttc ctcgttctgt tcgaaagtca cttcctgaag
    43981 cgcatcgtaa atgttgcttt cttcggcggc agtgagatca tactcgtcaa gaatcgtttg
    44041 ccttgcggct acgttagaaa tgaatgtttc ctgttcctgg acatccacta tgttgtcaaa
    44101 gctgtttttc acttcttcca aggtactgac attctggctc agcacatata ttccggacat
    44161 gaccaagaca agaatataaa tgccttgttg aagcgcgctt gaaaacttgg cgacaaaagg
    44221 acggagcgaa aagactacag cttcattcct ttgtccggag cggtactcgt tatattcgat
    44281 ggaattcgtc atattgagaa tgataaccat atagaagatt gcttgtcctc cgaaaacaag
    44341 agcgccgaat atgcacatcg aaatcaggct taccgggaaa atcggtaagt atcccatcaa
    44401 gagaaacatc agataccctg ccgttaaaac gataaagccg gttcccaaaa gctgttttct
    44461 cgagaatttt ttggccatca aaggatatgt gctttggaca agcacattaa caatgaaaag
    44521 aacaccgacg aatatcgtga tcgttgatcc gttatatcca agttcgatat agaagaaatt
    44581 gtatccaaga gcggtcaata aaccggatcc gacgctgtaa agcatcaagg ccagtgacgc
    44641 ccatagcaac tggtcattgt ttttgatgac ctggaacatt ttcttaatgg ttactttttc
    44701 cggactcgcc tgcatctcat cctgcggttc cttgactccg aaaaccgtta aacaggtaca
    44761 agccaggaaa atgataacga atgtgatcgc aattgtttgg taaccagtga ccatgtttcc
    44821 gactgtcacc atcgggatga tcgcatttgc cataatggcg ccgactgcgg caaatacgac
    44881 taccattgtc gcaatttgat cgcgttgttt ttttgttctt gaaagtgcgg gaagaattga
    44941 ccaatatgga atgtcattaa gtgtaaaggc catttcccaa agcaaataca tcatggcgaa
    45001 gaaaaacaca tatccccagt tattccagaa atgcggtctg acgatgaaaa agatcagaat
    45061 cattatgctt gtcagtatac cgccgataag gacccaagga cgatattttc cccatctagt
    45121 atgggtgttt tcaacaattg atcccatgat cggatcattc acggcatccc atacccttcc
    45181 gaatatcaag atgatgccga tggctgaaaa ctgcgccgtt gacaatccca aaccggaata
    45241 ttgcacataa gtcatcaaaa aagtggctat gagttgatag atcatatccc ggccgatgcc
    45301 accgacactg tagttccatt tgtttctacg taaaatcttt tgttcaagtg tcaagctgtc
    45361 tgttttcatt ttatcctctt ttcaatttat atatggcgtt ttaattatat cagtaaccaa
    45421 cgcaaaataa aagagtatcc gctttttaca tccattttta gccatattga ttaactgaat
    45481 aaagaaaagc gtcgcacaaa gcgacgcttg tttttataac tattttaatc ctttttacgt
    45541 aaaaatcaga agcaaaactc cgaaaacaac tgctaggatc agaattaaaa ccgggaaaaa
    45601 caccaagaag gcagcgatga tcatagcttt tacatcattt ttttccggag gcgtttcctg
    45661 aagattttta agcgcctgcg ctttttttct ttccttctct tccttagttt cccgataacg
    45721 catattactt catatccttt gtcttctcat aataatggca agcgacgaaa tgtccgggca
    45781 gaacttcttt gtattccgga accagctggg cgcatttttc ggtagcgagc gggcatcttg
    45841 ttctgaactt gcatccggat ggtgggaaga tattcgaagg aatgtcacct tctagaatga
    45901 ttctttccgg ctgaacctcg tcggtcgtgg gaatagcgga catcaaagct tttgtatatg
    45961 gatggagcgg attctgatag atttcatcag atggtccgag ttcgaccatg gttcccagat
    46021 acatgactcc gatacgatcg ctgatatgtt tgatgacgct taagtcatgg gaaataaaca
    46081 gataagtaag attctctttt tcttttaatt ccgccaacaa gttgatgatt tgggattgga
    46141 tggaaacgtc aagggcgctg acggcttcgt cgcaaacgat gaattcggga tccagggcca
    46201 aagctctggc gattccaaca cgttgtctct gaccgccgga aaattggtgc ggataacggt
    46261 gaagcatata atgcgccacg ccgcattttt ccatggtttg gaggacataa tcttccagtt
    46321 cttttgatcc tctcttgtaa agaccgtgtt ccgcgacggc ttcgccgatg atctggccga
    46381 ttgtcatgcg ggagtccaaa gatgaatatg gatcctggaa aatcacctgc agtttccttc
    46441 tcagcatgcg catatcttca taactgagtt tattcaaaat gattcctgat tccttgtacc
    46501 aatccagttt ggcttggaat tccggtttca tagcataatc gttgatcgca accaggtttt
    46561 taccatggaa gctgttgacc ttctcgaaag tggcaatgat ttccggagtg aaataactgg
    46621 cgtattttcc gtttagtccg tcgatgacat cgcggtaatg cttggcaaat tccacagttt
    46681 tctttagaag ttctttgatc tcttcaatgt tttggcacaa aatcagactg ccaaccaaat
    46741 gggaagattc tctcagatag cgagatgact gtttcttgta gtcaaaagac ttttgcctta
    46801 aaagcgcaat cttatgtgcc aaatctgctt gtttgctttc cagtttcttt ttttcctgtt
    46861 cttgaagcgg agattttaac tgtttgtcaa tctgtaataa ttccgcatca aaagctggaa
    46921 tcttgctgtc gacttccttg ctaagataaa agtaatgttt ggctttttgc tgattgtacg
    46981 gcaatttgtc aaccatcgtt tcgacatatt tcaacgatgt ttcgccgacc aaatcgccat
    47041 aataggttac gctgccgctt gtcggctcgt aaagttgcag gatagtgcgt cccaaggtgg
    47101 atttcccgca gccggattca ccgaccaaac caaaggtttc tcccttgtag atggtcagat
    47161 taatatcttc gacagcacga atatatattt ggttgcggtt catgaaagtg tttttcttca
    47221 atgggaaata ttttttcaga tcctttacct gaatcaaagg ctccctattc ttcgctgtgt
    47281 ttcccacttc ttcgcacctc gctttccggg taaaaacacc ttacgccatg tcccggttcc
    47341 acttcgatgg tctccggttc ctggttatgg catttttccg ttgcatactt gcatcttggt
    47401 gaaaacttgc aaccaaccgg caaatccagc ggatgtggaa cagatccagg aatgacatcc
    47461 agttttctgt ttttgtcatc gcttaagcgc ggtatcgaac gcatgagtcc ttctgtgtaa
    47521 ggatgcatgt attttgatgt cataccgaaa ataacctttg caggtgcgtt ttctacaact
    47581 tggccgcagt acatgacgac aacatcatca gccatttcat tgatgacgcc taggtcatgg
    47641 gtaacaaata tgattgatgt tcccaactcg cgtttcaaat cattcatcaa gcgcagaatc
    47701 tgggcctgaa ttgtaacgtc caaagcggta gtaggttcat cggcaatcaa taatttcggg
    47761 cggcaggcta aagccatagc aatcatgacg cgctggctca tgccgcctga gagctggaat
    47821 gaatatgtat cggcaatctt ttcgggattt gaaatcccaa ctgatcccaa taactgaatc
    47881 gtgcgttcgc gggcttgttc cttgttcatg tgttggtgca acatcagaac ttcgccgatt
    47941 tgtcttgcga ctgtcatgac cggattcaaa ctggtcatcg gctcctggaa aataaccgat
    48001 attttgttac cacggatttt acggatttcg tcttcgccat attccgagat tttttctcct
    48061 tcaaaccata tttctccttc atggattttc tggtttgatt cgaaaagttt cagaatgctc
    48121 atgactgtga cgctttttcc gcttccagat tcgccaacga taccgagagt ttttccttcg
    48181 tcgactttta gcgatacacc gtcaacggct tttatcaagc ctcttttggt tgtgaaataa
    48241 gtatgtagat tatttatctc caataaactc atatctcgca cctacctttc attgttcttc
    48301 ggatccatcg catctctaag agcgtctccg accaggttga tgctaagggc ggcaatgata
    48361 atgcataatc ccggcaaaac ccaacgccac caatatgtat cgaacacctc gcggtcttgg
    48421 gctactgtca aaatattacc ccaagaagga tttggcttct gaacgccaaa gcccaagaac
    48481 gatagacctg cttcggtaag cagcgatccg gcatatccca atgtcaagtt aacaagcacc
    48541 aaactgttaa cagcagggaa aatatgtctc caaatgatat gtttcttttt gatacccaaa
    48601 gctcttgaag ccaaaacgta atccctttct ctttcggaca ggatttgtcc tcggataagc
    48661 ctggccaacc ctgtccatga caaaacgcca aggacaatca ttatcattag aattctggtt
    48721 atttcgctcg tgtcaaccgt ggcattaagc aacaaataag atagggtgat ggcaaatggc
    48781 aggaacggga aggaagaaac aatctccgtg aaacgcatca gaatattatc gacccatccg
    48841 ccgaagaatc cagaaaccat tccgatgaca gtaccgataa caactgatat aataacagct
    48901 acggcaccga cagtcatggt tattttaccg ccatggatca gttgcaacac gaagtctctt
    48961 ccggactcat ctgttcccag caagaagcct ttaagacccc aagtctcaat attaccatcg
    49021 tcggtgacta aatagttttg gtaagcattg acgaatattt ccgaaacgtt tccgtcaatg
    49081 tcatcaggta aattcaattg gttgagatcg ttgtttcccc aagtgtacac atatccgtca
    49141 ttggttaaaa ccacgatgtt ttcatatccg gcttgaatgt ccttgacatt tgttctggct
    49201 ccgtcaggaa catattgtac gatttctccg gaaagattga ctacatcaat caattgtctg
    49261 ctggtgcttc cccagtcata taagttgccg tctgaatcta tggcaaaaca gttttcttcc
    49321 gtcaaggcaa cttttgtgac gaaaacagat ccgtctgtca attccacagg aacgctggtt
    49381 tttacttcag aagcggtacc gacgatcctt atggttccgt cttcaagcaa aataatcaag
    49441 ttattgttgt tcgcaacggc ttgaacggct ttgattggat ttcccaaagt atcggtataa
    49501 atataaggag atcgtctcat cggttgtccg acaatgttcg ttccgacgcc ccatacataa
    49561 acagcaccgg attctgtaat agcaattgta cgatcatatc cgccttcaag gctaacaatc
    49621 ggatcggcaa gcaatgcctc ggcatacctg tcgtcgatct gggcctgttg catatgatta
    49681 taaccccaca aatatacgtt tccttgatcg tcgatgcaca tcaagtgcct cgtaccggcc
    49741 gatatctgaa cgatgttggc gttttgaacg gcttctggga ttaccttaat atcataaata
    49801 ttggatcccc aaacaaatac gttcccttct tctgacaaac cgatggaaaa ggctacgccg
    49861 gagtcgattg atactactcc ttctttttcc aattccttcg gataactcag gtaactgtag
    49921 tttggcggga gattcgtatg aaatgcgcta ctgcgataca aatccagcgg gaaaaagatg
    49981 gctaatccga atgtgaaaat caacatcagg ataaatccaa ctaatcctat tatgcccagt
    50041 ctgttgtgaa aaaatttctt tgcaatcact ctgcccgggc tttgcaatgc ttcttcatcc
    50101 aaaatacttt tgatattctt ttctggaaca aagaggcgtt taaacatttc gcatattgat
    50161 ttccatattt tcttcaatgt tttcatccgt ttatccccct ctactccaac ttgacgcgcg
    50221 ggtcaaccaa cgcgtaactg atatccatga taatgttggc cagaagagac agaagagcat
    50281 aaaacatatc caaaataagg acaacattgt agtctttgtt gttcaatgct ttgatcaaga
    50341 cggtaccgat gccgttccat acgaagattt gctcagtaat ggctgagcct gaaaatagtc
    50401 cgacaacgga ataagacatg atcgtgacaa caggaatcaa tgcatttcta aaggcatgtg
    50461 tataaatgac tactttttgc gacaagcctt ttgatctggc tgttctgata tagtctttgc
    50521 ttaaggcttc taatacagag cctctcacgt agcgaattgt cccagctaaa gctccgatcg
    50581 tcaaagtaac aacggggagg aataggtatt ttaaataaga tacataatac tctggcgtac
    50641 cgggtgattc ataaggcata ccgccggagg ggaaccaacc taaacgcaaa gaaaatatat
    50701 aaatcagagt caatccgata aaaaaggtgg gcattgacat tccaatcaat gaaaaaactt
    50761 gccagccatg gtcagaaaaa gaatcctttt tgacagctgt cttaattcca acaggaatag
    50821 aaattgcaaa agcaatgact attgaaaata cgttaatcag aaccgaatta agtaaaggtc
    50881 tgctgataac atccttaaca tcagtcatcc aataggttga atagccgaaa ttacccgtta
    50941 tcatatttcc catccaaata aagtattgga tatacagggg cttatcataa cctagtcgga
    51001 tataccaaga atcatatagt gtttgataaa tatcaggatc tctgattgca ccgggcggaa
    51061 tcatatactt aatagggtca ccaggcatca atttcataat cacaaacagc atgactgaga
    51121 ttatgagaac gaccgggata agagatacca gcctccgccc catgaattta agcattattt
    51181 tctccttttc cgaaaaggaa ggagagtacc aagagctact ctcccctttt cttgttaata
    51241 gaatttcaaa ttattcagaa tctgtatggc tattgttatt ctcagtcacc aaagtacatc
    51301 gtgtagatag cctgtgtcca atcgaagaac ggtgttgttt ctactccatg aaggttagaa
    51361 tcatagacat cataatactg attggaatac agaggaacgt tcggaataag ttcattccac
    51421 aaagcctgat attgtctcca caagccgagg taagtttcat tgtctccagg ttcggtttct
    51481 ctcatagcaa caatcagagc gtcgagatct tcattgtcca ttccttggct gttgatccag
    51541 gtgttaagga agtctgaatg ccagctgtaa tatggatcgt aagtggttcc aaagttggta
    51601 gcaaggttga acaagcagta agttctgtct tctccaagat cgaagccaaa gtaatagtgg
    51661 tcaagaagtg tgtcgaatgt gccgatcgtg atcgtgtatt ctgcaccaat cagaggcata
    51721 ttgaccatga atttgtcgcg gagtgatgtg gtaacggtat tgttttcagt accgaaatgt
    51781 ttaattacca aacgatcgcc agaggcgttg tatctgtagt atgcattgtc ggaagtagct
    51841 ttcgtagcat cccacaaagt tgtaccatca gattcatatt tccattctgt ctgatcaaga
    51901 agagcattgg ctgcactgat gctgaagtca taggtattga ggttgtcttc tacccattct
    51961 tcgctatcct gatacatcca ttgtgccaat ccgtattcgc tgtaaacgat cgagccgtat
    52021 ccaccgagaa cctggtcaac aacatattga cggtcggtga tataagcaat agcctgacgg
    52081 actttttcat cctgagttgg gccaaagtcg catgtcatgg caagcaagcc atatccatta
    52141 cggttgtagt aatttgtttc tgcagttgca gcagcttcag cagcttcgat tttgcttcct
    52201 tcgataacgc cggtgaccaa atcgatttca ccactaatga ccaagtcaac atccaatgtc
    52261 tggttgactc tcttaatgat tacgctgtcg attgtggcat tgtcgccctt atagtcgcct
    52321 ttgaacagag gatcaagttc aagggttacg acttggttaa caaagctgac aaatttgtat
    52381 ggtccagctg ttacagtcgg atagtaacga tagccgtcta cgtcaacgat gttcgcgatt
    52441 ttcaagaatc cataatacaa tgtcgaacca gtttcgtctg aatcgatgta gcaggatccc
    52501 attgcgagtt catgcattgg cattggtcca taagacacat aagcggtctc atagaaataa
    52561 ggaagctcgg cagcgtcgat tgtcaatgag aatgtgtatt catcgatcag tttgacacct
    52621 tcgaagtaaa tgccttccga tgttgatcct tctggatcgt tgtaagcaga ataaccaagc
    52681 aagccttcgc ctgaggttgt gtcagctccg ccaacttctc tccagtcggc tgatgcggtg
    52741 aacagaacag agaacacgaa atcttcagca gtaatctgta cgccatcgga ccaatacaag
    52801 tcatcataaa tcgtgaatgt ataggtttta tttccgtcat catccgtgga tgttgcgaga
    52861 ttgctaacta ctgtgtcatt gatgacgatt tcgccagcag gtgtgacagc gtatgtcgca
    52921 tagccataaa tcaagcttct aacatagttg tcatatgagc tgttaccgaa acctgatatg
    52981 aattcgccat tcatttcagg aacaccaaca acgagttgct gtggttgacg ctctgttgtt
    53041 gtagttgttg tttcttctcc ggcggttgtt gtgccgcaag cgaacaatgt tacaacaagc
    53101 attaatgaaa cgattacact taatactttt ttcattatcc ttctctcctc gttaaaattt
    53161 tattcttgcc gaatatacaa cagttatata tgataggggt agtaaagtca ataactttat
    53221 ctgaactttt tctgtgataa cgctttcttt tcagacaaaa tatgccagtt ttgccctatt
    53281 ttgtgcgatt tttgcgactt ttaatgcgct attttgagcg caaaaaagcg accaatgaaa
    53341 gaaaatgatt cgcaatgcaa aatgacaatt tttttcttgt gtgatgaaga tatgtggaaa
    53401 atatataaag aaagaaatca tccgttattt ttgtaagata aatcgttatt tttgttttca
    53461 cataatcggc ttttttaatt aaaaatttgg ttatatatat aatataagga tagaataacc
    53521 ttaaataata agattaaaaa aaggtttgta agcacaaatc tattagctca tctagatttc
    53581 ttaacttctt ttctatatat attctaccac attttgagca ataagcgcaa tataataaag
    53641 gtgattacat agtttttgat gcatgtgatt ttgtgcgatc gattatttct tcttatatcg
    53701 gtgttcaaac caatataatt attattataa ttatttttgt catgttctat ttttacatgt
    53761 ttatttgtaa gcgctttaat gttataattg aaacggattt gaaatctttt ttttgtcaaa
    53821 aaatacgatc gggggattaa aatgagcaat aatttcaaag gcaggagtct taaaacgtta
    53881 cttgattaca cacaggcaga aatctacgaa ttgttggata ttgccaccga tttgaaagat
    53941 aaaaaacacc ataataaaga tttcacccca ttaaagggta aaaatgtcgt attattattc
    54001 gaaaaagatt cgacaaggac tagatgtgct ttcgaagttg gcgccttaga tcttggcatg
    54061 ggtgccactt atttaggacc aacaggatca cagatgggga aaaaagaaag catcgcagat
    54121 accgccagag tactcggaag aatgtatgac gggattgaat accgcggata ttcgcaaaaa
    54181 atcgtcgata ctttgtctgc ttatgccggt gttcctgttt ggaacggttt gacaaatgaa
    54241 taccatccaa cccaggtttt agctgatctt atgactattc gcgaaaaatt cggacatctt
    54301 gaaggattga ctttggcata tttcggcgat gcaagataca atatgggcaa ttcactgatg
    54361 atcgggtgtg ccattatggg aatgcatttc cgggccgtgg ctccgaaatc attctggcct
    54421 gatcagaatc ttgtaaaaaa gtgtcaagaa atagcgggtc gttccggagg ttcgatcacg
    54481 ctcacagaaa gtcctgcaat aggagccaat gacgcagatg tggtctacac agatgtatgg
    54541 gtatcaatgg gagaagcgga tacggaatgg caaaaacgca tagaagtgat gaagccatat
    54601 agagtaacta aaataattat ggataaagca tccaaaaaag ccattttcat gcattgtttg
    54661 cctgcttttc acaatacaga tacaattatt ggaaaagata tttttgaaaa atttggcatt
    54721 gattgtatgg aagtaacaga cgaggttttt gaatcaccac agtctgtggt atttgacgaa
    54781 gctgaaaacc ggcttcacac tatcaaggct atcatgtacg cttccttggc ggaggattta
    54841 taatgaaaat agtcattgct ttaggcggta atgctctggg aaataacccg gaagaacaaa
    54901 tacaaaaagc tgcttctgcc gtcaaaccgc tcgcaagttt aatcaaaacg ggacatcaag
    54961 tgacaatagc ccatggaaac ggtccccagg tgggtatgat caatctcgct ttcgaaaccg
    55021 cttcgctgac agacccaaag caaccaagca tgccttttcc ggaatgtgga gccatgagtc
    55081 agggatatat cggctatcat cttcagacct ctttgaaaaa agaattgtcc atcggcaaaa
    55141 tggaaaatcc ggtagtcacg ctcgtgacac aggttgaagt atctaaggaa gatccggctt
    55201 tttccaatcc gacaaaaccg atcggttctt tttattccaa ggaagatgca tctgaattag
    55261 caaaagccaa aggatacgtg atgaaggaag acgctaatcg cggataccga cgtgtcgttc
    55321 ccagccccat gccgatcgat attgttgaaa tcgattcaat caaaaccttg gtcaatcaag
    55381 gttgtgttgt tataacggtt ggaggcggcg gaattcccgt catacgcgat aaagaaggtt
    55441 accaaggcgt agctgctgtc atcgacaagg actttgccag tgcgaaattg gccgatcttc
    55501 ttgattgtga catgttggta atattaacag caatcgataa agtgaaaatc aattttggta
    55561 caaaaaacga gttatcactc gacaatgtat ctgtcgttca actgaaaaaa tatatcaaag
    55621 aaggccactt tcataaaggc agtatgcttc ctaaggtaga ggcagccatc aagtttgtcg
    55681 aagcaagaca aggaagaacg gccgtgatcg cctttttgga cgaagccgtt gaagccgtta
    55741 acggcatggc cggcacactt attcataatt gaaagaaggt ataaatatat gagaaaagat
    55801 cctgtaaaag tactgatctg gggcttcgga gccatgggct cgggaatggc cagaatgatc
    55861 atccgcaaac aaggtttcga cattgtaggc atttgcgacc gcaacccgaa tgttgtagga
    55921 aaaagcattt ttccgctttt gaaggaaacc aatccttacg accacgatgt cgtagtaaaa
    55981 gacgatattg atgctctttt atctgaaaca aaaccggaaa tcgccttact ggctaccgat
    56041 tcctttacaa aaaaagcctt tccgaaactg atcaaactaa tcgaagccgg atgtgacgtt
    56101 atttccactg ctgaagaaat ggcctatcca aaggcaaatg aaccggaatt ggcggaagag
    56161 ttagacaaag cggccaaaaa agccggcgtt accgtactcg gtaccggcgt caaccccgga
    56221 atgatgatgg atttgcttgc catcgccctg actggcgtga tgacagacgt cttcgatatg
    56281 aaaataagcc gtgtaaacag cctttctccc tttggcgaaa cggttatgca ggaacaggga
    56341 gtcggactta cacctaagga attcaccaaa aaacaggcga ccggagaaat cgccggccat
    56401 gtcggtttca gggaatcggc gatgatgatc gccgatgcca tcggccttag cattaaaaaa
    56461 ttcaatcagt caatgacgcc gataatcacc aacatcgacc gtaaatctcc ttatggtttc
    56521 gctgccaaag gcaatgtctg cggcgtggca atgaaagcaa atgcagagtt ggaaaacgga
    56581 atggaaatcc aacttgacca tccccagcag atcgaaccgg caaaaggcgg agtcacaacc
    56641 ggagattaca taaagatcgc cggacttcct cccatcaatc ttgccaacac tccggaagtc
    56701 gagggcgggc ttggaaccat cgccatctgc gtcaacatga ttcctattgt tttgaatgcc
    56761 aatcccgggc taaagaccat gatcgatctt cccgtcccga gagctatcgt cggagatgtc
    56821 cgcaagctga tcgaaacgaa gtgaccgtca tggtagaaaa aaatacctgg gtccaaatcc
    56881 acaaaatact tctcaaacca gaagaaagaa cggctaatct accggatgac accaaaaaag
    56941 tccctttgga aatgtgggta aagggatttt tgcttgcgga tgcaatgata ggtgacattg
    57001 tcaaagtcaa aacgcgcaca ggacggatcg aagaaggcac tctaataaaa gccaatccgg
    57061 cttatctgca taatttcggt aatttcgtcc ccgaacttct caaaatagac gacatggtca
    57121 aacatgccgt gtttggaagt gacgaagatg aatgacatga gttatcaagc attgatcgaa
    57181 cgtaaaaacg agattatgaa aaaatcggtc ggaatcgatt attcaaaata tgaaaacggt
    57241 aaaatcggtt tcgattatga aagattgttg tccgattctg gatatgatct ggaagaaatc
    57301 atgaaaattc aaaaggcgag cggtgttggt aacacccctc tcaaagaact gaaaaacatg
    57361 acgatgttgg cgagaaaagt cgctcctaaa ggatacgggg cccggatttt cgtcaaagac
    57421 gaatcgctaa acgattctgg cagtttcaaa gccagacggg cttcgatatc ttgttatcac
    57481 gcgaagaaaa taggttacaa gggagtcatt gccgcgactt ccggcaatta tggcgccgcc
    57541 gttgcttccc aagccgctaa atacggactt aagtgcatta tcgtccaaga atgtttcgat
    57601 tcgcgtggga taggccagcc ggaaatcatc gaaaaagcga gagcctgcga agcttacgga
    57661 gcggaagtcg tccaattatc ggtcggtccg gaacttttct acactttctt gcgtttattg
    57721 gaagaaaccg gatatttcaa cgcttctctt tattctcctt tcagcgtggc gggaatcgag
    57781 acactgggat atgaaataat ccaggattcc ctgtctctca ccggaatgga accgtcagct
    57841 attgtcgtca caaatgccgg aggcggaaat cttaccggaa ctgccagagg cgttttaaag
    57901 gcaacggaca gaaaagtcca gatgatagcg gccagcgtca atctaaaagg attgcacatg
    57961 gcaagcgatc acgatttcaa tcgtaagagt tttaccaccg gacataccgg attcggcatt
    58021 cctttcgtta ctttgccgga tcggagcgac gttccccgat cggccgcaag accgctccgt
    58081 tacatggatc gctatgtgac agtcaatcaa ggcgaagtgt ttttcatcac cgagactctg
    58141 actgcgttag aaggaatcga acgcggtcca gccggcaata tttccctggc cgccgctttt
    58201 gcactatcaa aggaattcaa tgaaaacgaa gtcattgtag tccaggaaac ggaatacacg
    58261 ggagccggaa aacatcacca ggctcaatta gatttcgcga gacaaaacgg tatcgatatc
    58321 tattttggca atccttccga ggaaattccc ggtcaatcaa ttgttttacc ttcggacctt
    58381 agtcttctca gaacggatga actcgatctt ggcaaactgc gtgattcttt tatccataac
    58441 gccgtcaaaa aacatgattc gctcgcaaaa tccgatataa aatttttggt agacgaaaca
    58501 agggcaacgg aagaattcgt caaaaacaca ctttccaatc tcggtatcgg aatggaggaa
    58561 tgaagatgga caaaaaaact atcagaccgg acgattatga atccaggagt attcatctaa
    58621 aaaaacttac tgatgatgaa ctaaaaaatt acttttggac attggccgaa aaagctgttg
    58681 atcccttgat caatcttgca caaaaaaaca cttctccggc aatcgaacgt tccgttcttc
    58741 ttcgcatggg gttttccagt atcgaagcta aagccatagt cgagaaaacc atccaaaacc
    58801 aactgatggg taaaggcgcc ggacatgtcg tttatcgcta ttcccaaatc aaaaacatcg
    58861 gtatcagaga cgccggcctg gaattgatga aagactccgg gtggtcatta gtcagcgaaa
    58921 gtttcggggt aaaaaagtca tgaaaccaaa cgacaagata tcgcttgatg aaattttcaa
    58981 agaccttgac aaatacactc cccgtcgcca cggatggaca tggagaaagc ccgtgcccgg
    59041 tcaaatcttc gacaaccaga aatacagcga tacttccgca tcgctcaaaa actatgttcc
    59101 attaccggct gccaaatatt ttggaaatat cgacccgcaa ccgatccagg taatcaccac
    59161 tgagatagcc tccggaagat tcgaggatga cattcgcaga atgcggatgg ctgcgtggca
    59221 cggagccgac cacatcatgg tgatccggac agcggggcaa tctcatttcg acggattgat
    59281 cgaaggaact cctcagggta tcggtggcgt tccgatctcc cgcaaacaaa tccgggcgca
    59341 aagaaaagcg ctcgacatga tcgaagatga agtcggacgg ccgatcaatt atcattctta
    59401 tgtatcagga gtcgccggac cggatatcgc agtcatgttt gcggaagaag gcgtgaacgg
    59461 cgcccaccag gatcctcaat ataatgtttt ataccgcaac atcaacatgg tgcggtcttt
    59521 tgtcgacgcc gccgaatcga aaaagattat ggccggagcc gatatgttac aaatagacgg
    59581 agcccacaat gccaacgcca ctgccatggt ggcatggaaa gttatgccgg aactaatggt
    59641 ccagcatgcc atcaacagtc tgttttccgt taaatccgga atgaaaaaag aaaatatcgc
    59701 tctttcaacc gtgcctccga ccgctgttcc tgcgccggac ctcaaagtga atcttcctta
    59761 tgcagtggcg ttgagagact ttttcgaagg ttatcgtttc cgcgcccaga tgaatacgaa
    59821 atacatggaa tcatcgacaa gggaagctac tgtcacccat gttctcaaca tgatgatatc
    59881 caaactgaca agcgcagaca tccagtcaac aatcactccc gatgaaggac gcaacgtgcc
    59941 ctggcatatg ttcaatgtcg aagcctgcga tacagccaaa caagctcttg tcggaatgga
    60001 cgggctcaaa gagatggtgg aaataaaaaa agacggcatt ttaccagcaa aagtacgcga
    60061 actcaaggaa agagccgtat tattctttga agaactactg gctgaaggcg gctatttcga
    60121 agctgtccaa aaggggttct tcattgattc cggcatctat ccggaacgaa acggcgatgg
    60181 catcagccga aatttaaatg gcggtgttgg tgccgatacc gtatatgagc gcgataaaga
    60241 ttatttcgcc ccggttccgt ctcatttcgg atacaacaac gtaagtcaat acaatttgtc
    60301 atcaaacgac aatccagccg tactaattgg cggatccact tttgagaacc gagagaaaat
    60361 ccagtatata gacgaacttg ataatgagga caatgtgtat ataaggatgg gggaaaccgc
    60421 caaatacaga gattcctcaa tgattaagcc ggaagtagag tggcttggcg acggtgttgt
    60481 agtaattgat ctgacattac cttgcggatc ggattatgcc aaagccgccg gcatcgaaat
    60541 cgccaaaagg atgaaccttt ccgatgccga agtcattcac acggaaatct tgcatccggc
    60601 cgaggcaacc agaatccaag tcaaaggaac cgtgaatttc gatattgatc tcttgaccct
    60661 gacattgccg aaaaagcctg atcttttaac cgatcaggaa atccgtgatt acgtttctct
    60721 tcatcctttg acgatcgtcg ccggaactgt cggcgaagac gaacattccg tcggattacg
    60781 ggaaatcatt gatatcaagc atggcggcat cgaaaaatac gggatcaagt gcatttatct
    60841 gggaacttcc gttccgatcg ccaaactggt ggatgcggcc atcgaaacaa aagccgacgc
    60901 aattcttctg tcgacaatca tttctcatga cgacatccat tatcgcaata tgaaaaaagc
    60961 caatgattac gccattgaaa aaggcgttcg tgacaaaatc atcatgatcg caggcggtac
    61021 ccaggttgtt ccggacacgg ctatcaaaca aggtatggat caaggtttca gccggggaac
    61081 aaaaggacaa gatgtggcca gttatctcgt aaaaagacat aaggaactga taaaaaagtg
    61141 aaaatcgacg cattggtagc cgaaatcgga tccacaacga ccgtcatcag cgcttttgac
    61201 aagctggact gcaatccggt ttttttaggt cagggattcg ctccgactac ggttttggaa
    61261 ggcgatgtca acctaggact gaccaatgca attatcgatt tgaaacaaaa acttggtatt
    61321 ttagaacttg aagccaagga aacttttgcc tgttcaagtg ctgccggagg acttaaaatg
    61381 agcgttcacg gtctagtcta cgacatgacg gtgaaagcgg caagggaagc cgctctaggc
    61441 gctggagcca atctgaagtt agtcagcgcc ggcatgctaa atgatgaaga tatcaactat
    61501 atcaccaata gcaatctgaa catcatcatg atcgccggag gcgtcgatta cggcgaaacc
    61561 gccacagcta tcgcaaatgc aaccataatc gccggattgc atctttcaat tcccgtcatc
    61621 tatgccggaa acattcaaaa ccaacaaact atcgcaaaca tatttgtaaa agaaggacaa
    61681 agcgattatc tgtttttgac aaaaaacgta tatccaagga tagacgaact ggaaacagac
    61741 gaagccagaa aaataattca atcagtcttt gaaaaacaca tcatcaaagc gcccggaatg
    61801 gaaaaagtcc gggaaatgat cagtgaaaac atcattccga ctcccggagc cgtgatggat
    61861 gcggcattgc tcatgtatgc cgagataggc gacctagtca cattcgacat cggcggtgcc
    61921 accaccgatg tccattcggt gaccaatgga agcgaaagaa acgaaaaaat catgcttgga
    61981 ccggaaccgt ttgccaaacg gacggttgaa ggcgatttag gagtatacgt aaatcatgac
    62041 aacatcatca agatgatcgg taaagaaaaa ctcgccagtg atttaggtat cagcatatcc
    62101 caacttgaat ccatgctcgc cggttatctc gcaatacccg taaaatccca ttatgatatg
    62161 gtcatgcggc ttgccaaaga agccctgaaa tacggtttag aaagacatgc gggaaaatac
    62221 atcagtcttt acggcgccag cggcaaaaca accatggcgg agggaaagga cctttccgcc
    62281 ataaaatatg ttatcggcac cggcggtgtt cttgccaaac tggacggaaa aaatatcatc
    62341 tccactgttt tacttcaagc cagcgacact atgatgaagc caaatccgga tgccaaaatt
    62401 ctgattgaca aagactatat catggcggct tgcggagtat tgtctcacaa gcatccaagc
    62461 gctgccacca cgcttcttaa aaaaagtctc ggaatcgggt gataccatgt atccacaact
    62521 tgtaatcgat gtgaaaaaac taaaaagcaa taccgaaaag gtattgtctt tatgccataa
    62581 acacggaata tcatcgacta ttctggttgt aaaggtattc gccggttttc ttccgattgt
    62641 cgagatactc gcccaaaatg aattcgatta catcggcgat tcccgtctgg aaaaccttga
    62701 aagatttcaa aatatatcca agccaaaatg cttgttaaga atcccttcac caagccaggc
    62761 ggaaaaaacg gtccgttatg caaatctgtc gctaaattcc gaacttgaaa ctatcaaggc
    62821 tttaaacgaa gcggcccaaa gacagaacaa gatccatgat atcatcttga tgttcgatct
    62881 tggcgactta cgggaaggta ttttcttcat ggatgattac ttaccaacag tcaaacagat
    62941 tcttacttta aaaaacattc atctcaaagg cattggaacc aatcttacgt gttatggcgg
    63001 tgtgatcccg acagtggaaa atctaacgga actcgtcaat ataaaaacca agatcgagac
    63061 tcaattctcg atcagtctcg atattatttc cggtggtaat tcttcttctt ttgttctttt
    63121 ggaatccgga aatgttccga aagacatcaa caatctccgc ttcggggaaa tcgtctacat
    63181 gggcagggaa acggcttatg gcaattatct cgaaggccta tataaagaca ttttcactct
    63241 ggaagccgaa atcgtcgaac tcaaacaaaa accgagttac ccgatcggag tgattggtat
    63301 gaattcattt ggggaaaaac ccaatatccc cgaccaagga accatgacaa gagctattct
    63361 ggcgatcggc aaacaagatg tcatcctaga aaatctgact cccaaggata aatcggtttc
    63421 gattatcggc ggcagcagcg atcatttgat tgtcggattg tcagaaggaa gccacaaacc
    63481 gggagacata attgccttcg acgtcaatta cccgggtttg ttgcacctga tgaattccca
    63541 ttatatcgac aaggtgatca aacaataaaa aaagcggtcc agaggaccgc ttgaatctag
    63601 gctttgttgc gaatatggat gaccgtgttg atcttcttga cttcgaactc ttccatcttt
    63661 tcaaagaagt tcaacggacg catgttacta ccgtagttgc ggggatgaaa tcccggaaac
    63721 tttctttgga acagttgggc gatcaggctc caataagccc atccgtcatc ttccatattg
    63781 tcattaatga tgtttttgag ttccgccttg atcttggata acggcggaat tgaactctcg
    63841 gactttccat tggttttctt ctttgatgtc ttgtcatcat ctttcttatc agtacaaggg
    63901 gttatttccg ggtgttcggg cacaaccatc ttatcgagat aggaaaacgt ctcgcaagag
    63961 ttgacaagag aaatcggcgt tttgctttcg cccatcccga acacgaacat gtcgtcttcc
    64021 ctcagcctag tcaccagttt ggtaaaatcg ctgtcgctcg agacaatgca gaaactgttg
    64081 actttcccgg aataaagaat gtccatggca tcgattatca gtgtgaagtc ggaagcgttt
    64141 tttcccgatg tataggaata ctgctggacc ggagtcaatc cgaattcgct gattctttgt
    64201 ttccaggtat tgagctgcgt cgttgtccag tccccgtata cccggcgata gttgatcttg
    64261 ccgtatttgt tggcctcgtc gatgatgatt tcgatatatg cgggagaaat gttctccgcg
    64321 tcgatcagaa gagctatgct tttattttct atttccattt ttatacctcg tttcaattaa
    64381 ttatattatc attttacgat cgataaaaca agttatcgca ccaaatgatt cttaatgaca
    64441 aggagaaagc agatgaaaaa caacgaactt gaaaaatggc tggaggaaat aaccgcttgg
    64501 tgttcccccg acaaggtggt tctttggcat ggtaccaaag acgagtatga ccgactggcc
    64561 caaagcatgg tcgatgacaa aaaagcgatt cggctcaatg aaaaattacg gccaaattgc
    64621 cttttgttcc ggactgatcc gtccgatgtc gcccgcgtag aacaacgcac gttcatatcg
    64681 tcagtcgaag aaaaggatgc cgggcctacc aacaattgga tcgatcccaa gattctcaaa
    64741 ccgacaatgt gggaaaaata ccataattcg atgaagggcc gcactatgta tgtgatcccc
    64801 ttctcgatgg gacccgttgg ttccaagttt tccaaactgg gagtcgaatt gaccgacagc
    64861 gcctatgtca ccctgaacat gcatatcatg accaggaccg gcgacaaaat catggaaatg
    64921 atcgacaaag gcaccgagtt cgtcaaatgc ctgcattcga tcggctatcc gttaaatgaa
    64981 ggcaataatg atcccttgtg gccttgcgcc ccgatcgaag aaaaatacat atcccatttt
    65041 ccggaagaca acatgatctg gtcctttggc agcggctatg gcggcaacgc cttactcggt
    65101 aaaaaatgtt ttgcccttag aatagcttcc aaccaggccc gtaaagaaaa ctggctcgcg
    65161 gaacacatgc tgatactgaa attaacttcg cctgaaggtg aaaccaaata tatcacggga
    65221 gcattcccaa gcgcttgcgg gaaaaccaat ctggcgatga ttagaccgac cctgccggga
    65281 tgggttgccg aaacagtcgg cgacgacatc gcttggatca gacttggcaa ggacggcgga
    65341 ttctatgccg tcaatcccga agccggtttc ttcggagtcg ccaccggaac atcctgggaa
    65401 tcgaatccca atgccatgga atccatcaag aatgatacca tattcacaaa tgtcgccttg
    65461 acagacgacg gcgatgtctg gtgggaagga atgacaaaaa aagctcccga tcacctgatt
    65521 gattggcatg gcaacgactg gacgcctgaa tcagggggaa aggccgcaca tcccaactgc
    65581 cgtttcacgg ctccggcggc taattgtcca tcaatcgcca ataactggga agacccggaa
    65641 ggagtcccgg tttccgccat gcttttcggt gggcgccgtc cttcaaccat tccgttggtg
    65701 catgaaagtt ttgatttcaa ccatggtgtg tttcttggtt ccatcatggg ttccgaaatt
    65761 acggccgcga cgatttccaa cgatatagga aaagtcagac gcgacccgtt tgccatgttg
    65821 ccgttcatcg gttacaacat cgccgattat gtaaatcatt ggcttgagat gggtaaactg
    65881 gccaacaagg acaaacttcc gcgtatctat tatgtaaact ggttcagaaa agatccggaa
    65941 ggccggtttt tatggcccgg atacggcgag aacatcagag ttctcaaatg gatattcaac
    66001 cgtcgggata atctagccga tgccaagaaa actccgatcg gttacgtacc aagaccggaa
    66061 gatttggatc ttgaaggctt ggatatatca gatgaaaacc tcaaaaagct gttggatgtc
    66121 gatgtaaaca gttggacaaa tgaagctgct tcgatccgcg attactacga aaaactttat
    66181 ggcgacacct taccgcaaga acttggaaaa cagttatcgg aacttgaaat gcgactgaaa
    66241 gcctgacatg caaagaaggt ttccttattt aggaaacctt tttcattaaa acaaaaacct
    66301 tttccaatat ttagcgcttc aaatatgtta taatatgaga atatgaaacg ggggcatgct
    66361 ggatgtcgag aatcatcagg gaaaaaggaa gcctctttct tgtcttttta ttcttttttc
    66421 ttttaacgga agctatcact ttccgttggg taggcttcac aactttctca caatttctat
    66481 atattgacat aataatggct ttgatcatcg gttcgattgc cattcttttc cgatcgaatg
    66541 tcgtcagttt ggtaatttat tccttcatat acggaatcgt catcgccttg ttcatgatca
    66601 atgtgacaat gtacgatatc tactacgatc ttttttcgct gcaacaactc caattgatca
    66661 acgaggcagc tactgttttc aatttcgcat ttctttccat ccccagcata ataatctccg
    66721 taatcctttg tcttcttttt ttgacaatta acattctgtt ggtcatttat ttcaaaaaac
    66781 atccgcaaaa ggttgatcgt ttccatctta agacggtttt catgttcgtt gtcatcaaca
    66841 tgtgtgcaat caccctgttt gcgagcggta ttccttcagt atcggaatat aaaagccaaa
    66901 gcaacatcac gacttttaaa cgggcttccc tggaaaaata cggaacctta ggttattatg
    66961 ccaaggaagc ggaagacatc atcctgcggg aatacaacct gaccgatgac ggtgataata
    67021 acgaaacccc cagcaatatc gtctatgcgg accagacgga ttatttcggt cttctggaag
    67081 gaatgaatgt catcacgatc atgatcgagt ccgggcaacc attcggtata tcggaggtgt
    67141 tgaccccgca tctttacgat ctgactcagg aaggtttgta tttccctaac aactacagcg
    67201 aaaacaaaac gaacatttcc gaagtcgcag cgatcactgg caattatccg tctatcaatt
    67261 tcctgccagg ttcctatgac tatgaaatgc cattcgcggt tccaaatatt ttggctgata
    67321 catacgaaac gatgtatttc cacgacaatg tcccgagttt ttattcgcgc ggcgcgcttt
    67381 tgccccaact cgggttcgaa aacatttatc tgcacgacga tctatatccc ggggaagaca
    67441 tctggacctg gaacggagat tacactttgg acagcgagac aatcgaaaag atccttccaa
    67501 tcctgacatc ttcaggagaa ccgttttacg ctttctggtc gactttgtcg actcacggtc
    67561 cttacaattg cggaaggaat aacatcataa gctttgctga aaagaattat tttgccagaa
    67621 tcgacgccgc tgaggatgcc ggattgtgga caaatgttct agcaaatgga gaagctaccg
    67681 acgcttcaaa aatcagatac taccaggctg ccatgatgaa tctggatgac gccgtcggaa
    67741 ggctgcttga ggaactggaa aacaacaata tgttggacaa tacggtcatc atcctttacg
    67801 gagatcacaa tgtttactat catgatctat ccactaagat gtatgccgat tcgggaaacg
    67861 aatactacaa catggaaatg tatcacacgt tcttctgcat atacaacaaa accctgaccc
    67921 aggcttatct tgaaaatacc ggaagcgact ctacttcgat cgaggatttc acttccccat
    67981 acagcatcgt tcccacttta cttgatttat tgggatattc ctatgataag aacatttact
    68041 tgggagattc aatcttttcc gggaagatgc aggtgtttta ttcgcacaaa ctgtccggcc
    68101 tcttcaacaa catcatttac agcaacgacg gaattgacat tgattactcg agcatatccg
    68161 tggacgacga ttacattgac agtttccggg aagcctgcga ggaactgatc gtcaggctcg
    68221 aatatgtcaa ttacgcttat tacagcaccc agacaaaccg agattgatta caaaataacc
    68281 gtgtctgtca aagacacggt ttttcttggt ctagcataac agtactacta taatacacga
    68341 gtatgaaacc acgggggagg tgggcatcat gcctcatatt tatgggccgg aacatattat
    68401 ttatctattt gttgtcatcg ttttaatggg aatgggcttt ttcctgatca accgccatgt
    68461 caagacggaa aagcaattgg ctttggcaat caggctgatt ggtggcttgt tgctaatcat
    68521 gatcatttgg aaccgcctca gcatcgctat tttgagagac ggattcaatg aacttctgcc
    68581 gggatcatat tgcggaacat cgagtctagt attggctttg gcagctatat ttctgaaaaa
    68641 ggatcatccg gtcttccact gtgtggctta tatcggactg cttggcggat tggccactct
    68701 ggcttatccc gatttcatcg gacaagccga ttccattttt tatccgatga cgatctccgg
    68761 gcttgcccat catacggtca tggtctttct ggttgttgtc atgttgacaa caggatattt
    68821 gaaaccaatg ttccttaaat ggcattatgc cgttttggga ctagccctat atcttgttta
    68881 cggacttttt ctcattaaag tgcttggata tggagaagca atgtgtataa accgaccatt
    68941 gctggacgat accaatcttt actgtttcca gctaggagcg atatatcttc ccgttcatgc
    69001 cgcttttctc gccatatgga atcttgtcgg taaaaaatac cggattggcg ctatgctggc
    69061 aaaagtcgcc agctaaaata atacttatat tcggacataa agattcgctt ttttgcgaat
    69121 ctttttaatt aaccaaaaat ttaagcattg tttttgacaa tttaacggaa aaaaagtatg
    69181 attatagcgt taaagggagg tctttttatg ggtaaaaaaa ttcttatcgt cggcggagtg
    69241 gccggcggag catctacggt agccagattg cgccggatgg atgaaaaagc ggaaatcatt
    69301 atgttcgaaa gaggcgagta catctctttc gccaactgcg ggctgccata ctatatcggt
    69361 ggcaccatcg gttcccgcga cgctttgatc gttcaaagcg tcgaagacat ggaaacaaaa
    69421 ttcaacttgg acatcaggat tttatcggaa gtcacgtcga ttgatgccaa aaacaaaagc
    69481 gttaccgtga caaaccataa aacaaacgac acctataaag aaaactatga ttttctggtg
    69541 attgccaccg gagcctatcc ggtcaaaccg ccgattccag gaattttgga agcgaaaaat
    69601 cttttcactt tgagaaacat tcccgatacc gacgacatca agggattcat caccaataat
    69661 catccgcata aagccgtcgt aatcggcgga ggattcatcg gactggaaat ggcggagaat
    69721 ttgaaacatc agggtcttga cgtcacaatc gtagaaatgg ccgaccaggt aatggctccg
    69781 ctcgattatg aaatggccca gattgtccat caacatctag ccgaaaaaga agtcaacctg
    69841 attttgaaag acggtgtcaa gtccttccaa gaagccggga aaaaagtcca gctcaccagc
    69901 ggaaaagtcc ttgattccga tatcacgatt ctctcgatcg gagtcaaacc ggataaccag
    69961 ttggcaaaaa ccgccaatct ggaaaccgga cttcgcggtg gtatcatcgt aaacgaacac
    70021 cttttgacat cggatccctt tatctatgcc atcggcgatg cgattgaggt cgtcgacttc
    70081 gttaacaaag gaaagaccat gattccgctt gccggacctg ccaacaagca ggcgagaatc
    70141 gtcgccgaca atatctgcgg catcgatgaa atttatcccg gaacaatggg aacggccatt
    70201 gccaaagtat tcgacctggc ggtaggtacg accggcaaca acgaaagaca gctgaaatct
    70261 accggggtca gatattccat catccatacc catccgggtt cgcatgccgg ttactacccg
    70321 ggttcttcca ccatatcgat gaaagtcctt ttcgatccgg aaacggaaag aatactaggg
    70381 gctcaagcta tcggaatgga aggcgtcgac aaacgcatcg atgtcatcgc taccgccatg
    70441 atggccggaa tgaaaataac cgaactcaag aatctcgaac tttcctatgc ccctcctttc
    70501 tcatccgcaa aagacccggt taatatgatc ggctttgtcg ctgaaaacat attgaaagga
    70561 ttagtggaaa cattccagtg gtacgatgtc gacgagctcg tcagagaggg ggaattcctc
    70621 cttgatgcca gggaaacaat cgagcatgaa ctgggaacca ttcccggatc tgtgaacatt
    70681 cccctttccg tattgagaaa caatctcgga aaattaccga aaacaaaaac catatacctt
    70741 tactgccagg ccggaatccg cggctatatc gccgctagga ttctcatgca aaacggcttc
    70801 aaggtcaaga accttgacgg cggatacaaa acctaccaga tcgtcttcga tcccgctcac
    70861 gacgccggat ccaaagcaat cgacgatgcc ggcaccatga aaatcgagga ggaacaaaaa
    70921 cccatggaaa acgaaaccaa tccgaaaatc accttgtccg tcgacgcctg cggtctccaa
    70981 tgcccgggtc cgatcgtcca ggtttacaaa aacctcgaaa caatgaagga aggcgaagtc
    71041 ctagaaatca aggcttccga tcccggattc aaaaaagaca tctggaaatg ggccgaaaag
    71101 accggtaaca ccgttctcga tctgaaaacc gaagacagag taatcaccgc cctcgtccgc
    71161 aaaggaattg caaaggcaag caaacccgta gaaaactttg tgacatcgga caaggaaaac
    71221 acgaccatcg tcgtattcag tcaggatttg gacaaagcca tcgctgcctt cataatcgcc
    71281 cagggaaaag ccgccatggg gaaaaaggtt tcgctcttct tcactttctg gggtcttaac
    71341 atcctacgca aaccaaaaaa agtcagagtc aagaaaacct tcatcgaaaa gatgtttggc
    71401 aagatgatgc ctcgtggcgt cgacaaactg ccgatttcca acatgaacat ggccggcatg
    71461 ggttccaaga tgatcaaggc tatcatgaag aaaaagaatg tcgattcctt acaggtcatg
    71521 atgaaaaatg ccatgtcgat gggtgtgaag atcactgctt gcgccatgtc gatggacatc
    71581 atgggcatca aaaaggaaga actgatggac ggcatcgaaa tcgccggagt agctacctat
    71641 ctagcggaca cgacggaagc caaccacaat ctgtttatct aaaaaaatgg ttcgccatca
    71701 ggcgaacctt tttattctcc gataatcttg accaaaaccc ttttgtcacg cattccgtca
    71761 aattccccgt agaagatctg ttcccaggga ccaaagtcga gtttcccgtt agtgacggca
    71821 caaacaactt cccgcccgaa aatttgtctt ttgatatggg cgtcggcatt gtcctcataa
    71881 ccgttatggg catattggcc atagggtttt tccggagcaa tcttttctaa gaatcgctcg
    71941 aagtctgaat gcaaaccgga ttcgtcatcg ttgatgaaga cactggctgt gatatgcatc
    72001 ggattcacca gcaccaagcc ttccattatt ttactttccc tgatgcatcc agaaacctgc
    72061 tttgtgatat tgacaaactc gcgtcttgct tcggtatgaa accatagttc cttgcgataa
    72121 cttatcattt tgctcgcctc catttgacaa taatatttta gtatctagcg aaaacaatga
    72181 caaccttttt ataaagaaaa cccggataaa tcgggttttt tcgtttattt attggctttg
    72241 gcgatcattt cttcgatttc cgataaaggt ggaaccggtc ctgctgacag gagttttccg
    72301 tcgatgaaaa gcgccggtgt tctcataact ccggatttgg ctatttcggc aaaatcggtc
    72361 acatatgaga cttcggcatc aatcccgagt ttttccactg tgtgattgac catttggagc
    72421 agacgtttgc agttggcgca tccgccgccc ataacaataa ttttcattta ttctttcctc
    72481 ctttctagac aagatagatg taaaaagcat tgaataaata tcccatcgca atgataccaa
    72541 cggtgatgat accgacgaat gtccagagca gtttcggctt tacgactctt ctcagcatga
    72601 tgatcgaagg gagcgacaaa gcagtgacgg ccatcatgaa ggaaagcact gttccgatgc
    72661 caacgccttt caagaccaaa gcttcggcaa tcggcaatgt accgaaaata tccgcataca
    72721 tcggaattcc gaccgctgtt ccgatcaaca ccgagaaggg attgttgtca ccgagtacgg
    72781 cttcgatcca attttgggga atgaatccat gaatcaaagc tccgatgccg acccctatca
    72841 gaagataagg ccatactttt ttaacgatat ccgtaacttg ttgccaagaa tatttagctc
    72901 tttctttttt ggtcattaca gtttcaggca tttcttccat cgcaacttcg ccatctgctt
    72961 tcacgaagtc ggccacatat ttgtccattt tgaaaagacc gatgagcgta ccgccgataa
    73021 cggcaatgat aagccccacc aaaacataag caatggcaat cttccagttg aagatacttg
    73081 ccaataaaac caccgaggca agatcgacaa gcggggatga tattagaaag gaaaatgtca
    73141 cgcctaccgg taatccggct ctggtgaaac caatgaagat cggaatcgag gaacaggaac
    73201 aaaacggtgt cacggttcca agcaaagcgc cgatgatgtt ggcgggaagt cctttgaatt
    73261 tgctcatgat agcccggctt ctttccggcg tgaaccgcga ctggatccac gaagaaataa
    73321 aaataagcac ggaaagcaaa atcagaatct ttatcacgtc ataaaagaaa aatgtgagga
    73381 atgaataagc ggcggatgtt tcggatattc cgaagacacg gccaaacagc cacttgacta
    73441 tgtcatcaag ccataacatt ttcagtaatt ggtcattcat ccaggcaaag atgtctttta
    73501 taatttgcat gtttttttag tctccaggag ttcttcaaga tatttgatgg tttctttgat
    73561 tttttcctta ttcacaaaat tcttgttcca ggttccttcc ttgcggctgg tgacaagccc
    73621 ggatgctgac aacattcgca aatgatatga caaggtgggc tgggatatcg gcaaactgtt
    73681 aatgagagta caaccgcatg attcgccttc gagtaatagt ttcatgatca aaagccggtt
    73741 cggatccgac agggctctaa aagcctccgc catttgattt tccatgatgc gcctccttat
    73801 atagatattc atctatattg atgatcatct atatttttgc ataccttttc cggaatgtca
    73861 aacgatattg tctttttgtt ttcaggtaat aaaaaagcgg catcgccgct ttcttacttt
    73921 gcacattttg tccttgtttc agccagaatc caactgtccg gataagtcat tctcatatat
    73981 tctgagaaag cggatttttg atcaagcaag cctttcttca aaatgacttc tgtctctgca
    74041 ttttttatcc attcacgatt ttcattgttg agatcttggt gcctgatgat tatcaagttg
    74101 ccaagctttt cctgtttcag gatttcttca taaagatctt ctattaccac aaccccaatc
    74161 acgtgaaccg aagacctgct gacaaactca ttcggaatca ttgttttttc gtaattgtct
    74221 ttgtcttcgc attgataaag aaatgctttc tttccgctat ataatttttc cagagcgtcg
    74281 ggaaaatgtt ccatgtagta caatttcttt tctctgttat acccataggt gtactcgaaa
    74341 tgactgatac cgtaaaaaag cgccattggc aacaaggttg tcatggacac aagacgaggt
    74401 ttatcaaaat aattcggagt attcggctcc aaatctttga ttcttgcaac cgaagaacaa
    74461 tgatagaaca acagacccac ttccagctct ttataatctc gccttgacaa aatcaaggta
    74521 ttccatcata tttttatcat cctgttcatt gtatgtgcaa ccaagatatt ttgccacgga
    74581 tttggcagta tttcggaaaa tccggcagga ttcctcaatg gctttccaga tatgatcgta
    74641 gttaccgtcc gggattgtaa gaaggtaccg gttatagcaa tcttcggaca aatatctgtt
    74701 aaagtacttg ccccatttgc cgacagaaac ggcaaaatca tttgtcacgc caatatgcca
    74761 ggcaagcatc cggtttagtt ccttatgaac ggtttcgtaa tacattctga tgacataagg
    74821 catttggtct ctggcaatgc ctttggcaac attgttgaga caccaaaaaa attcgttaca
    74881 acatccggca tattctgctt tcgtcggttt tttgattaaa taatcccgat tgttagaaag
    74941 cgaaattttt ggcaatatcc catctttgtc aaacaaagga acggtcaaac tgtcaagccc
    75001 gtattcctcc tgcatggctt cgatactttg aatttgcaga tcgattctga caaaatcctt
    75061 gaaaagcatc aaccaagcat aacttctggt gggatcattt ttttgaccga aagccagatc
    75121 gtttgaatcc ggttcctgga gcatcagcaa ttccccgaag atctttagcc aggttttatc
    75181 cttgatgaat ggcaaagtgt ttgtgacgac atacacgatg tcgtaatcac aaaaatcatc
    75241 tatggcgatt tccggatttg ttctcgatcc gttcatataa accgccctga tcctttcatc
    75301 ctctttcgca atatgcaaga tcaagtcgta tatttcttgt tccgaacgca tcttgctcac
    75361 ctcttatttt tgaatcattg ttcccggaca taaaattcca tgccgactcc ggtatctctg
    75421 accatggcgt cttttggata tttcatgagt ttccaaataa cataaaaatc cccgatgcaa
    75481 ctacaaaaat gcataccgag catagtgacg gcgaatccgg ccaaatagat atcactggca
    75541 aaacaaaata ccagaaacag cgtgccgaga ataaccgccg gcgctatccc gacaacaaaa
    75601 tattgccatt tggaaagata atgaccggga gcggaagcac tgaacgccca accatgaaac
    75661 tggtatttga ctttggattt ggtaaaaaca tgaaaaaaca gaccatgaat ggcttcatgg
    75721 atgatgatca tcaagaacag tcccaagaca agaagcgtaa gccaatagag ctttgtcatc
    75781 gaggcgttca tcacatccga aaagaaacgg ataatgaatg gtatgaacgg caacaacaat
    75841 accaacgaca tgatgttgat ccatacaatg agttttttat tatgtttcat gtcgatctcg
    75901 taattcatca cataaccttc tggcaaaatc ttagtatcaa tcattattat cttcatcctt
    75961 tcgcttataa acaattatca agattaaccc agatgacagc tcccgacgga tgtttgtatt
    76021 ctatcggctc gggatagcgt ttcgaatttg tcaagaccca tgtgtaagta ctaggatatg
    76081 gcaaacaatc tatttctgtt ttcggcacca tgtgcttgtc tgatgtgttg gcatattctg
    76141 atagatccaa agggatgcaa tcgaccagat cagcttctcc taccaccaga ccgctttgac
    76201 ttctgatcaa agctattctt cctctaatgg tggtatttct gccgcggatc tcccaagtct
    76261 tcttaccatc tagaatataa tcgatccacg gtgttcttat caataatccc ttcattgtcg
    76321 caatctccat catattattt tcggcgggta cgctttctcg aagtcttcta acaaatgcaa
    76381 aagaaaatca tccaaatact ctctggcctt gtcgctttgt tcgcggggga ttccataata
    76441 ggcgccagcc acggctccgg tgatggcacc gatcgtatcg gaatccccac cgatcgatat
    76501 ggcacacctt atcgcatcct cgaaacatgt tgattcaaaa aaagctttta acgcttgcgg
    76561 agtggttccc aaggcggtag cgtcgaaaac ataatccttc cggataccat ccaaggtaaa
    76621 atccagcgga tagtaattgt ctataatatg tttttggatt tcttccttgg attttccgtt
    76681 tcttgccaga aacacagcaa ctgccgtagc ttcagcggcc ttgattccat ccggatggtc
    76741 atgggttatt tccgtcacca ttcgggaaat gtcaataacc tcctgcaagg tctttccgac
    76801 aacgccacag gcggaaacgc gcatactggc gccatttcca tagcttccgt atggtttggg
    76861 atcatcagag aaaagccagc ggtaaaagtg atgcccgtat ccggcgttcg gatagatgcg
    76921 tccgagtttg cgcattgatt ccttcgcttt atccggcaat tcaccatact tgtttttcca
    76981 gtcaagcagg gccctgccga tggcaattgt cattacggtg tcgtcagtga aaaaacagtt
    77041 tttcttgaat agttcaaaat ctttggtctt gatattgttg aattcgtaaa ccgatccgac
    77101 gatatcgccg atgattgccc cgatcaaacc cggatgccgt ctttcgcgtt tcttcttttt
    77161 ccgtcttggt attctttcaa caagtcaagt atttcatcgc tattttccaa aacaaaagcg
    77221 acgaactctt tgtttgcgaa tatcgctgtc atattatttc cttcggcaag caaacatccg
    77281 gcatccgaaa aaggcctatc cagttcgatg atatccttgt tatgcaaaga aacgaaaaga
    77341 atatggtttt ggaagaagta aatggtgagc ggcatcgcct tatccaaaaa atgttttccc
    77401 atgaacatgt acccattgta tttctttaag aaaggttctg gaaactccgc ttcgtaatct
    77461 acttccaatt catccctttt ccgatataaa aactgccgga atctgggatt tttttcgtga
    77521 aaaagggcaa aaaacaaaag gaggccgaaa accagtccag agaatatata gagcacatac
    77581 aactgccagg cgtatccggt cctttgccac aggaatattc tcaataagaa tgtcagtaac
    77641 aatcctcccg ttatcggaat gatgaaatcg attataggga ctcttttttt acggtttaga
    77701 ttgcttctga aaaaaatatg cacatattcg ctcaggttgt ctccctttgt ggcgacgtga
    77761 tcgataatca actcgggtgt ttcccaacgg gaagccatta tggtcgtggc atcgtcaaaa
    77821 ctgtattcgt tcttaaaatc cggttgcgaa tattccttca ccagttcgtt ttggatgtcc
    77881 ttatatgatg cgtccacgac ttgtgccgac gtgtaatatc cttccggtcg catcagttcg
    77941 atcatgttta cccttaaacc agaatgtccg attcctattt gcatcggcag attgctttcg
    78001 aatacttcgc cctcgaaacc ggtataagtg ttcttgtccg tcttccatac gggaacgtac
    78061 atgtccttca aagctttcat acattcgcgg tatgatgggg tgagtacgac acccatcctt
    78121 ttaatttcaa attgctcgct cattgattat tctccttctt gaaaaaggcg atgtaacaac
    78181 cactccttga aattgttttg cttgtttact tagccttgtg attgttgttt tctctcagaa
    78241 ttaccttgcc tttgcaagga atggcggtat tgtcgtgaat gacttcaccg taagaatcca
    78301 ctacaatgtg tccacgtttc ggatttttga tcgaatcgat gcgtcccttg atgtcggctt
    78361 cgacatcgga atattcaaaa gccagatcgc aaccaaccat ttcgcagtcg atcagtttaa
    78421 gattcttgca atagcatagc ggttgacttc cgcttatttt gcaattgatg agcgttagat
    78481 gttttgaata ccaggcaaag tattctcccg tgatgacgga atcggtgatc acgacatcgt
    78541 cacagtgcca aaaggcatcc ttggtgtcga tatgcgattt atctatcgtg acatgctttg
    78601 tatattgaaa ggaatatttg cctttcattt cgatgttttt gatatcgata tgcgttgaat
    78661 cgagaaacag gtattccgac tggatttggg aattctcaac ggatattttg cttgatttcc
    78721 acccgaattc gtcggatact atcctcgagt cggttatctt gatatcatga cattccctaa
    78781 tcgccttggt gccatgcaaa gtcagattgt taatggtacc gtttttcaca taccataacg
    78841 gggctctggt cgattcctcc atgattgtgc ccgtcatggc taaacctttt gtatgccata
    78901 aaggatagcg catggcgaat aggcaatttt caaccacgat atccctggct tcctttaacg
    78961 gcgattcgcc ttcggcgctt ttaaactcac atttgaccag tttggttgcg gtgaggttgt
    79021 ataaggaacg ttcctggtca taatgacgat tctcaatgat ttcctttgat tgtatttgca
    79081 tgttggctcc ttgtatgaat aaaataaatg gaatttatga aattactgca ctatccataa
    79141 taaggtccgt ttcggtttgt gtcaatagtc gtgttgcatt tttgcatgca acgggatgaa
    79201 aatgaaaagt ccggttctca aaatacgcga accggactgt cccaaccttt tttaaaaaga
    79261 cagacaaact ttattattta atgttgtcac gtaagaactt cttgtcttct tctttgacat
    79321 cgagtccgat cgttccgccc ggattcatta gataatccct gtcgaaataa tccttcaaaa
    79381 ctttgaagac tccgtattcc tttctgccta aatatccttc cagccaggga gcaaacatct
    79441 tgccgatgcc atggtggtgg cttaaggcgg cgccgctttt ctggatcgca tccaggatgg
    79501 tggcgtgata agccttgaat tcatcggcct tctcgatatg gccgatgaag atgaaataca
    79561 gattggctcc ttgcggataa gcatgggaaa gatgcgtagt gacgacggtg tccggtctgg
    79621 cgtggcacac ctttcttact tcgtcatgga ctttggccat gttggaccag ttgacggaac
    79681 actcaagcgt atcggtcgca atcccgaaat caagcagggt gtcacgtaga tacggatcgg
    79741 tgaaacgacc gtgttcccag cctttgcaga ctagtccggt caatgacctg gcatggtatt
    79801 ttctcgccac tttggcgatg ttatgtctga catttttgga aaaccccaat tcaccgtcgg
    79861 tgaatcccag cataagcgac cgctgcatcg gcaggaatcc tttcaggaaa aactttaaag
    79921 gagaatcgtc gacattgtaa agtttcagca tcatgtcggt ttcttcggcg tccgataccc
    79981 ggaaaaccga ggaatggccg gcttccgatt gcatcatttc cctggcagcg tccatggcat
    80041 cttcccaagt cttgaacatg taggaatacc gtttacggtt tttaggcata taacgaaaga
    80101 ctttcagggt cacttccgtc aaaacgccaa aggcgccttc cgaacccatc atgatctgat
    80161 taagattcgg tccggtcgac tctttcggat aatgacttgt attgatcgta ccgatcgggg
    80221 tggcgtattt ctgggacaag acaatgtcgt caatggtacc ataatatgtg ctgttctgtc
    80281 cggcacctct cgtcaccacc cagcccccga cggacgaata ttcgaaagat tggggaaaat
    80341 gtccgcaggt ataagctctt ttcgcgttga attcggcaat ggcattgtta aggtgttttt
    80401 ccaaatcagg accggacatt cccggttgca cggtgattgt ctggtcggtt tcattgaagg
    80461 ataggacctt gttgaagttg cggcgtagat ccagagacac tccgcctttg accggttcca
    80521 ctcctctggt aacggaagat ccgccgccat agacataaag cggaatttta tgactgacgc
    80581 aatatgcgac gatcgcttcc acttcttttg tcgtagacgg atacaaaacc agatcgggaa
    80641 cgctgtcata gcggtgctcg cggatccgca tgatgtcata aagcgtcttg ccgtacataa
    80701 cggaaagacg gtcataggtt tcataggacg aattatggat gccgacgatc ttttgcaaag
    80761 cgtcgatagc ttcgttatcc atgtttgaag gaatgacatc ggtgattttg tcgaaaccga
    80821 tatcgttgtg gtaatgagaa aaatcatcat ccgtcatatg gaacttttcc ttcatgtaac
    80881 ggtaaagcga cgctttagga tatttgacaa atttcggatc tccccaccgg gatatggagc
    80941 ggtaagaatc tttcgaagct tccgttgtca cccattccgg caaaaattcc ttatttttct
    81001 ttgaagccat ttttatcacc tatctcactc tcaaatattt tttgtagatc ggtcggaggg
    81061 ccggatagat ttccctatat atcctgtcat agatctgcat atattcttca tgcctgtttt
    81121 catcgggcat gaatgtcttt tcgtaggaaa ccatgttctt gacggcatcg tcgaaattgt
    81181 catatacacc aagcccgaca aagccgcaaa tgctggagcc gattcctgag gcttcgtatt
    81241 cggtcgtttt cttgacggga agcccgaata tgtccgctgc catctggcag atttccaaag
    81301 aattagcacc gccaccggaa atgaccaaat actcaatatc ctgtttggtt cgctttttaa
    81361 gcccgacaaa gccggaataa agcgtgaagg cgatgccttc gatgatcgaa cgatagagat
    81421 agtatttgtc gtgatccggc atgaagccga tcacggctcc tctcgcttcc ggaagtttaa
    81481 gaggcgcgtt ccaatacggt atcagcatga gtcctcccga tccgatcggg acatctttta
    81541 aatgcatgtt cagttcttct tcggtgctga tgcctttttc ataggctttc ttggcgtcgt
    81601 ctttagcgaa attatcaatg aaccaggaaa gcatccaaca acccctaaac acctgaagtt
    81661 ccggattgta aagcttcgga ttgatggccg gataggcggg taggaaagtg gccggctcga
    81721 tgtatttgtc ggtagaaaac tggatcgtgg cgctggttcc gaaagataag gaagccgaat
    81781 gaccgtccat gacgcctgaa cctaaagtct cgcaaccctt gtcggatccg cttgcaaaaa
    81841 gaggcaatcc ctctttcaat ccggtttctt cagaagcaat tttcgttata taaccgatga
    81901 gatcggtcgg atcgacgagt tctgtcagtt tgtcttcctc gattccgaag attggaaatt
    81961 gaagattgct tttcttcatc cattgcttgt gtttgttatc aaagggaaca tgcccgatca
    82021 tagcggcgct cgaatctttg tacactccgg tgagtctggc gtgcacaagc gcgctcagat
    82081 ttccgaattt ggcggttttt cgccaaagtt caggctcgtt ttccttgatc cagttcggac
    82141 ggcagatttc ccgctgacac tcaatagcgt aatccattcc gacaagtttg tagaggagtc
    82201 tttgtaacaa gggcaaacgc ttttcgcatt tcgcctttct ctggtcaagc cagatgatga
    82261 aatccctaag cggttttccc gattcgtccg tacaggtaaa agtgtcacgg atcgttgtca
    82321 ccgtcaccga agcgatgtca tcccacagct tgccggaaat ctccttcacc ccaagggatg
    82381 ccttgcaggc aaagaaccag tatttgtcga aagattgttc gcagtagccc attttcctgg
    82441 cataatacgg aatgtcgtac ttctctttcc tgataacgag aatctttcct tttttgtcga
    82501 ccagcatcgc tcttacgctt tgggtgccga aatcgaaagt caaaacaagc ggatccatga
    82561 tcccatcccc tttataaaag tatcatccat tcaatagtat atcactcgta ataacaatat
    82621 caatattgag accgagacaa ttttcgataa ctttgtatcc ccggccaact cttttatcca
    82681 gaacgatttt gtttatttcc ttcataacat caaaaatcct ttccttggga aaatccgccg
    82741 atgccttgat tggcacgacg ggaacatcgg aaaagattgt tttgacggta gacgatatcg
    82801 tccgtttcgg attggtcatt tcgtcggtgc cgtaagctat gccccgaacg catctgtttc
    82861 cggtgacgat atattttccg tctttctctt caacttttaa taggcaactg tttggacaga
    82921 caatgcatgt tatttccttc atgatcatca ccttgccaaa gtgaaactga tcgatccgtt
    82981 tatctgggaa aaatcaagcg cgatcgacag tttctccatc tccggcggcc ggagatgggt
    83041 gtattttctt ttgtagattg cccggccttc cgattctata tttagcgtga catctttttc
    83101 gatcgctttg gaacgaaaat aaaaatcgac tttgcctttg gcgcctatcg atacatgttg
    83161 cggaatcaca tacatgaaat cctcggaagc tgaaaccggg atgtattccc tgactttttt
    83221 ttggtaagtt gctgcttctt tgccggcttt ttccgccgta atggtgacgt aatcgaccag
    83281 atcgttaacc tgcatcgcgt tcccgcaggt gaaaacgcct tcgattccgg tcatgcagtt
    83341 ttcatctaca accggacctt tggttttggg atcgatcgga acagacaatg tttcggccag
    83401 ttcattttcc gggataagtc ccacggacaa aatcaaagta tcgcaaggaa tatgtttctc
    83461 tgttccttcg ataacctgca tgtttccatc aaccctggcc acatcgactg cttccaatct
    83521 gttttcccca tatagtttgg tcacggtatg ggatagatac aaaggaatgt catagtcttc
    83581 cagacactgg cagacatttc ttgtaaggcc ggaagggcgg ggcagtatct catagactcc
    83641 cacgacttcg acaccttcta atgtaaggcg acgggccatg ataagaccga tgtctccgga
    83701 acctaaaatc acgcattttt tggcgggaag acagccggca atgttggtaa aatactgggc
    83761 ggtgccggcc gtgaagacgc cgtatggcct ggttccgttg atgaagatct gtctcgatgt
    83821 tctttcccgg catccggtag atagcacaat tgtcttggtt gcgacttcca tgacgccttt
    83881 ttccgttaca tatttgatca tgaacaaatc gttttgtttc tcgattttgg tgacgaaact
    83941 ttgcagcgta gcttcgatgt ttctttgctt gaattcgtca atgaatttct cggcatattc
    84001 cgggccggtt agcttttcat tgaaatatat tagtccaaaa ccgtcgtgga tgcattgttt
    84061 caaaatcccg ccaagtcttt tttcccgttc gatcagaaat actttggcag agttgtcatg
    84121 agcgctgatc gccgccgcga gtccggccgg gccgccgccg atcacgactg tatcataatt
    84181 attcatgaac tgagcctcct ttttgcgact tgaaaactac cggggatccg atctcttttc
    84241 ccgcgacttc ttctaatgga atatgttcgt gttcggagat gattttggca actaaaggca
    84301 tgcagaaacc gccttggcag cgtcccattc cgggacgggc ccggcgtttt atgccgttta
    84361 ctgtcggaac cttgatattg cgattcaagc agtcgataat ttccccttcc gaaattcctt
    84421 cgcaccggca gatgattctg ccgtaagccg gattcctttt gatgagggct gtcttctctt
    84481 cttcggtcat gaaacgcaaa acgggatccg gttttctctt tggatcgaat ttagtgtttg
    84541 atcttacttc ccggctttct ttcaaaatat cgatgcccat cttggcgaca tctttggcaa
    84601 caagcggact ggtagtcaaa ccaggcgact gaataccggc gcaatggacg atatttctcg
    84661 tcttgcggcc tttttcaata tagaaatcct cttcgtatgt ggcggctctg gtaccggtaa
    84721 aataggcgat gatatctctt ttttccaggg ttggcacgat atatttctgt ttttgaaaaa
    84781 cttggtccaa ggattgggat tcggtggcga agttttcccg ttccggcgtt tcgtaggcat
    84841 tcggaccgat caggatattg ttttcgatcg tcttgatcac accgccgcct ttggaatggg
    84901 cggttttttt attgtgggag atgaattcga tcgaagtgat atgattgatt tcctgtccta
    84961 ttttcttgtc gaagatataa tccgatccgg agcgcggatg gatcgtgaag taacggtcgt
    85021 ccgccatttc ggcaatcttg tccgcaaacg tgccggcggc gttgatcaag actttcggat
    85081 atatcatccc ccggtttgtc ttgacggata caactttatc atccttaagt tccatcgaca
    85141 acacggcggt atcaagttcc actctggcac cgtttttcac agcgttttcc gcaaaagcga
    85201 ttgtcagttc gtatggcgac acgacaccgg actgcggatt ggatacggcg cattccactt
    85261 ccggattgaa cttcggttcc gttttgagga tctcttcttt accggccact tcggtcggac
    85321 aaccgatttt actgcggctt ttcgcgataa gccgtaagag cggtttgatg ggcttgttga
    85381 aacaagcgta ttgtcctctt ctttcgaaat gtacgccaag ctcatcgcat atatcgccga
    85441 aaatccggtt ggccctgacg acataatatt gtttttttgt tcctatgccc aaatcgacgc
    85501 cgggatggat ttcgccgtcg tttctcccgg aagcttgcat tgccagatcg gattctttct
    85561 cgaccagcat tgcacttaca tcatatcgca tgagttcacg caaaatggaa gcgccggaaa
    85621 taccgccgcc tatcaccaaa aaatccggtt tcaagccgtc tagtttatta tcgcatataa
    85681 aaggcaaacg cattttttcc tctttggcgt tttttagcgt tatatcattc aatactccga
    85741 tgtaacgttt cttgtccaca gccaaatacc cggcattaac aacatcttcc catttgtcga
    85801 gttcgcccgt caatctgacg caaccatctt tttcttcggc ttcaactccc ggataaagtc
    85861 cggccagttt tttcctcaaa cggcttatct tcataaaaac ccctcacgat ttcgtgtcgg
    85921 ccacttgacc gacatacagg catagtatat aatatcactg gaaaaaaatc aacaatttat
    85981 gataaaataa gtatcaaagg gatgatcatc gtggccaaaa aacgccttga cgagaacctg
    86041 cgcatgttaa tcctttccaa aacgcaggaa cttctccaaa gcaaatcttt cagcgacatt
    86101 tcattaaaca atatcgccaa gtattccggc ataagcaagg gcacgctcta ctattattac
    86161 aaaagcaaag acgacatcat cgtcgaaata tacggccagt cgatcgattc actatgggac
    86221 gagtttatcg aatgggtcac caataaagat aaaaacacat ctttacaccg ggtagccaag
    86281 ttcgtcatca tgaaaggcac gggttcttct tccatccggt tgaatctgat tagcgacgcc
    86341 gccaacggca acaacgaact caaaaaaaga ctgttggaaa aatacaacgc cttcgtgaat
    86401 gcgttctaca acaaactaag agaagacatg accgaagagg atgccagatt tgtttcatgg
    86461 ctgcttgtca tagtaactga cggagtcaat gtccaaaaat ccctgcataa ccccaaattc
    86521 gattcggaat ctttcatcag aatggctgaa gacttctttc ttaaagccga agaaagtcta
    86581 taaaaaaccg ataaaaatgc cggcataccc caaaggtaag ccggcttttc ttattttttt
    86641 ttaaaaaaat ataatgattc aaacctgttt ccggtcaaat atcaatattg aagcactgac
    86701 aaaaccaaca atcaaagcgc cgcaaataat caaggccgga acaaaatcag atgtttccaa
    86761 ggttcccatt aacaaactga cattgactga agcgatagtc gcaggaacat atttttggaa
    86821 tgtcgggaca atattcaata gtgtcatgac aacgacaagt ccaccagcaa aaagcaggct
    86881 accgaccatt ctcgaaaaca agatgccgcc cagaatcacg gaagcgatca ggaaaaggcc
    86941 gaagagccac aagccgaaga aagcaagaaa gccgttggag aaagagccga ttacgaataa
    87001 gtaatccgtt aaaaaatatg tgatcgcaaa tgccacaaaa taaacgatgg tccatgttat
    87061 gctagcgact gtaaacttgg caaagacgac cgtccggcgt ttcaagcctt ttgtcaggac
    87121 attcacaagc gttcctttcg aaagttctcc tgccattgtg ttggcaaata ctatgacaac
    87181 gactatcaag cccatctgtc cgacattttt gaaaaattgc gcaaagctgt caagagccgt
    87241 cggatcggca aggccgaggg tcgatggatc gataccagcg gccgcgagga tttccggcat
    87301 gtaaaaagca ataagcggat tcatcaatcc aaataataag aaaactatca acagtatcaa
    87361 tagcttataa gtacggaaca gttctgtcca ttcctttttc acaaacgcca gatatgcttt
    87421 cattttaccg cctccaagaa gagactttcc accgttggtt cgagaacctc gaattttttc
    87481 ggatacaaat cggctttaac cagccagttc agaatatccg cctttgtctt ttcgatattt
    87541 tcggcaccga tcagaaaatg tctgtcaagt tggccgataa tcgcaaaagg agactttttc
    87601 agaaaaagag cggcttcggc ttcggtctcg aattccagaa acaaccggtc gttgtgatgt
    87661 ttgtttttaa gctctgatac cgagccgctc aaaaccagtt tgccttcttt aagaacaaca
    87721 acatggtcgc agatgcgttc gacatcagaa agaatatggg tagaaaagat aactgtcgtt
    87781 cttcctttga cggtcaaaag aatatccagg atctccttgc gcccaaccgg atccaaagcc
    87841 gaggtcggct cgtcgcagat caacagctta ggctcgttta gcaatgcttg agcgatccct
    87901 aaacgttgtt tcatgccgcg tgagaaactt ccgattttct tgtttacgtt atccagaccg
    87961 acgagatgca acaattcctg ttgcctgatt atgaacaaat cggatggcat gccggtgata
    88021 tcggcacata attttaagta ttccttcggt tttaaataag aataaaattc aggaacatcc
    88081 ggcaaataac caacgacttc gttcgtccta gtctcaccgt attttacttc catcccgtcg
    88141 ataatcgctt tgccttcatc cggtttcaaa agacccagaa tcattttcat ggttgttgtc
    88201 tttccggctc cgttcggacc gacaaaaccg aaaatggttc ccgccggcac ctcaaaggaa
    88261 aggttgtcga cggcttttat gccattgaag ctttttgtga gatttttcac tgaaaggata
    88321 tccattctat tccttctttc tcgtgacgta tttattcgct ccggttttcc atccgatatg
    88381 aattccgtgc tcgtcaagaa aatattacca cagatagatg aaaagcgaaa ctattgtttc
    88441 gctcaaaaca atgattatgt tttctcatct ggctcatgaa taagttaaag tgcaaacaaa
    88501 atattatctg attgttgtta tttaagtgtt gtttacaaag ccgtgttctt ttcctacgaa
    88561 tccttgtata gggtggacat cttgattttc atgttatata attacatcaa atttaaacgg
    88621 ttaactcatc ggtttatccg tttgatcttg cttcataagc catatcaaaa aggaggccgc
    88681 gtcgctatgc ctaaattcac cggttctgac atgaagaaaa gattattgtc attgtttctt
    88741 atattcgttt ctttgtttct tttttcctgc aaaagcgtaa cggacacttc ttttacgaca
    88801 acaacccgga atgccgatga ggccatattg ttaagcaatc ttgataccat atttgaagat
    88861 gccgcggata tttccaatat ttacgggata tccgtataca aaaatgacga attcatcgca
    88921 agtgaattct acggactcac caccaaggaa tctaaaaaca atgtctattc cgtcaccaaa
    88981 agcatcactt cattattgct gggaatagcg atcgatatgg aatatatcga aagcgtcgat
    89041 cagacaattg gtgatttcat cgatctttct tcatatgaaa accaagaagt cctcagcacg
    89101 ataaccatca aaaacctgat tacaatgagc gcgggattgg tctgggattc cagcaatctc
    89161 gccggagaaa tgtccgaact aaggacggcg gaggatccgc tggctttgat tcttgagcgt
    89221 gatatagaat atattccggg aaccaatttc aattatagcg acggcgccgc gcatctgatg
    89281 tcgatcatca ttagccaggc taccgggatg agcgcccacg atttcgccaa agaatacttg
    89341 tttacccctc tgagtatttt caatacgatc tggaacaccg accgattaga caacaatatt
    89401 ggcggatgtg atttatatct cagccatgaa gacatgtaca aaattgctaa acttgttctt
    89461 aaccgcggcg aatacgaagg tacgagaatc gtatccgcaa cctggataga cgaatcgact
    89521 gccactcaga tacccgtaga ctacgccacc gactatggat tctattggtg gattaccagc
    89581 ccgggagatc aaaaaaggat ttccgccaga ggctggggcg ggcaacagat ttatatagtc
    89641 cccgatgaaa acattattgt cataacctct gccgacggat gggtatcaga caacattgct
    89701 cagacccaat ttactgcatt gcattcggtt gcggaaaatc aaatcattcc cttgttaacc
    89761 tcctatgacg attgatttat ggattaaggc aaaataaaag gtttctgatt gcattcaagc
    89821 aatgaagaaa ccttttttta ttatcttaat tcaagcatgg cgacgctttc cacatggcat
    89881 gtttccggga acatgtccac cggttggacc tgtttcaagg aatatccctg ttttgccaag
    89941 accgacaaat cccttgccaa agtatcggga ctgcatgaga tatagcatat cttatccaaa
    90001 tgcagtctga ctatgccttc caggcatttt tcggatagtc cctctctcgg aggatcgact
    90061 aacaagatgt cgaattcgtt ttcttccgga tcaatcgcac cgataaactc ggcggcgtcc
    90121 atctgataga aatgaatatt tctgacatta ttgaaacggg cgttctgggt ggcgttcttc
    90181 accgattcct tattcagttc gacaccttcc actttgcggg cgtgatcgga aaatattatg
    90241 ccgatagtac cgattcccga atacagttca agcactttgt cggttttctt cggcttggca
    90301 agttccaagg ctttacggta caatatttcc gtttgtttgt gatttatctg gtagaaggtg
    90361 ttggcggaga tcttgaattt cttacccaaa acgatatcga caatggatcc cggtccgtac
    90421 aaaactcttt cgaaattgcc aagcaccgtt gatgtggttc tcgtattgat attttggacg
    90481 attgtcgtga tttccggatg tttctctctc aaagcgacaa cgaaattgtg ccggccgggg
    90541 aacatttccg ttatggtcac aatggcgacc aaaatctgat cggtctcttc ggctgtacga
    90601 acatagatat gccggacgag tccggttttt ttgtcttcgt cataaggttc gatatgcatc
    90661 tgttgcatca gtgtccggca agtcttcacg atatcgttgg cgatgtcgtt ttggacatag
    90721 cacttgtcca cattgatgat ttcatgggtg ttttcggcgt agaatccccc catcactttt
    90781 ttgccttttt cggaaatcac catctgggct ttattccgat agtggaaagg ttctttcatt
    90841 ccgatgatgg gaagacaaag gtttgaatct aattttgcat ttgccagaag atgccttact
    90901 ttggcggttt tcagttccag ctgtttttca taagagagat gttgaagttg gcatccgccg
    90961 cattcgttgt aatacggaca aagcggtttc acacggtccg gggaatcgtt ttgccgggat
    91021 aacactttgc caaaaccatt cgcaaaaaaa cggatctcgg ctttttctcc caataacagg
    91081 ttcgggatta aataacgttt ccggttttta tgcaagacgg catacccgtc gtcattgatc
    91141 atttcggctt catataattc cggcatcttg tctctcctta attaccgtat atcagatata
    91201 ttatccatct tgccagacgt cttggcaaga tgcgattgag aaataccagg attttgtagc
    91261 cgatgccgac ggttcttacg accggcatcc ttcttctctt ggcaagcctt tggacgatcc
    91321 tcgccacata atcgggattg actccgtttt gctcgtcgtg ctccatcttc ctgaccgatt
    91381 tttccagtct tctgtgatat acggaatcct tgacgctcgg gttcttttcc ctggcggcgg
    91441 tgaatccggt tttggtatcg cccggcatga cggtcgatac ctggattttt tccggtttca
    91501 gttccatgga aagtccggtc gagaaaatct cgatcgccgc tttggtcatc gaataaaacg
    91561 tttggaaggg gatgacaatc tcgccggcta ccgaactgaa gttaatgatc ctgggctttg
    91621 tgcccattct caacaaagga atcatttccc ttgtcagcat gaacacggac ttgacgttcg
    91681 attcgaaaat cttcacgcat tgctcggtcg gggtttcttc gattgctccg ctgatgccca
    91741 taccggcgca attgatgaga atatcgacat gtccggattc ttccatgatt ttcgaaacca
    91801 attctttgat ctcttcttct tttgtcaaat cgcaaagcag gtatttgaaa tcgtaaactg
    91861 tcgtcgggta atgccgggag acaccgtata caatgtcgcc cgcatcgtaa aaccttttcg
    91921 ccatcgccaa accgatgccg ctggaggctc ccgttatgac tatcactcta ttcattcttc
    91981 acatattcct tcatgagttc ctcgtccgat tccggaagat tgttcaagac atcggcgatg
    92041 atctcaatcg cctcgtccat caatgcttcc gtatgcgtcg cggtatagct ggttctgatc
    92101 aggcattcgc cttccggagc tgccggtggt aaaaccggat tgacataaac gcctctttcg
    92161 taaagcaact tgcaggcaat taacgtcctc aacatcgaat aagtgtagat cggaatgatt
    92221 ggcgcaatgg aattctccat gatcttgatc ccattggcaa tcaagccttt acgcacatag
    92281 gcagcgatgt caagcaaagc tttcggcctt tccggttcgc tttccaatat ccgcaacgct
    92341 tctaatgccg tggcgaccga tgtcggtgtg atggcggcac agaagatgaa cggacgtgat
    92401 ttgtgtttga catattcgac gatcttttca tcgccgacca tgtatccgcc taaactggcg
    92461 agcgatttgg aaaatgttcc catgatgatg tcgacttctt tttccaagcc gaaatattcg
    92521 gccgttcctc tgcctctttt gccaaggact ccaaaaccat gggcgtcgtc gaccatgact
    92581 ctggcaccgt attttttggc caatctaaca atttccggca gattacatat atcgcctgac
    92641 atcgagaaga ctccatcggt aacgatcagg attccctgat cctccgtggc gctttgtagg
    92701 catttttcca aatcctccat gtcattatga tggaatctga ccatcttggc gtaactcagt
    92761 ctggccccgt catagatgct cgcatgattc tcccggtcgc aaacgatgag atcgtttctt
    92821 ccggcgatgg ctgaaatgat tcccaggttc gtttggaatc cggtagaaaa tgtgacacag
    92881 gcttccttgt tcaaaaatct ggcaagttcg gtttccagtt cgacatggct tgtcaaagta
    92941 ccattaagaa aacgggagcc ggaaaccccg gagccgaatt ctttcaaagc tttgaccccg
    93001 gcttcgatga cacggggatc gccggtcaat ccaagataat tgtttgatcc gatcatgatg
    93061 cggcgtttgc cttccattgt gaccacgata tcctgcttgc tatcgagcgc atggaaataa
    93121 gggtatacgc ctattttttt ataatcgtcc actgttgtga actcaaagca ttttttaaaa
    93181 agatccatat tatcctccgc agtataatgt tagctgttaa tcttcatcgg caacgatgtt
    93241 gccggtatat acagtaatgt cttccacatt ttgggaagct ttggaaaggt gggtctcaaa
    93301 aaacagtttg tcttcatcta gcaacatggc atatgccatg tcgtttacgg caagaatgtc
    93361 cagttcgcct gtattggcgt tccaggacaa tctttcggct gtgtcgaacc gatcgatccc
    93421 gtattcggga taatcggtgt attcgtctcc tccttcctcc ggttctgttt tttcttcttt
    93481 ggggtatgcc gccaaagcga tcccggcgtc gatttgatca cagtcaagct ctttaagcgc
    93541 ttcgttatat gaatccaaca tcgtgttgat tagtacacag ttttcgaatg cccgcatcac
    93601 gccttccgat tccggcgtga agctggtcga tttcagaaaa tcgtctcccg attcgacata
    93661 tactacatcg ggatcctttt tcaaaagaac caaagattcc atgagaaacg attccaaaag
    93721 caacgcagcc ttgggaattg acttgtcacc gtaaaaagcg tccagattgc gaactgatat
    93781 gaaaaggtcg gtgaagatct cctcgtattc gtattcctct ccgacttcga ccatctcgtt
    93841 ttcctttgtt tcgtcttcaa acggagctaa atcatccatc atcaagatgt ctgcgatttt
    93901 ttttctgatt tctcttaaca tttcatacct ccaaaaattg tttacctatt cggttctcaa
    93961 tgcgacaacc gggtctttct tcgctgccat cagggccggg atcaatccgg caatataggc
    94021 taaggcaatg cttatcgcaa ctaagaaaac cgcatgcagg agagacagtt tagcgacatt
    94081 cggcatgtcc gcaaaatgtt cgatgatgat attgattgga attatcaaac cgaaagcaat
    94141 ggcgattccg acgattccgg aaccaaaacc gatgactaaa ttctccgcat tgaatactct
    94201 ggatacgtct ttctttctgg cgccgagtgc ccgtaagact ccgatttctt tgatccgttc
    94261 caaaactgat atataggtga tgattgcaat catgattgaa gacactacca aagagatgcc
    94321 ggcgaatgcg atcaacacga tcgaaatcgt attgatgacg ccttggacca tggtaacaaa
    94381 agtggcggcc tgatcgacat attcgattcg cgaaatactg tcggcatcga actggtcgtt
    94441 ataggcatca agataagtct tcaggttttc tttggcatcg aaatcgttcg gatagatata
    94501 gatgctgttg acatcgccga tttcttccgg ataattcgct tcgatataat caactagcga
    94561 agaatcgtaa ccgaatccgt tataaagcaa attgacactc gagccttcct tgatccggat
    94621 aatgccggat atttcgatat cgatcgcatt ggaaacatcg gttgtttcgt catattcgcc
    94681 gacataaaga gtgaaatcct taccgacgat atcggaaaaa tagaattccg tttcattgtc
    94741 ggtatcgaaa ccaaggcggc tgctcaaagt cgttttgtaa atgcggttgt attcgtctac
    94801 gaccaatacc acctggaatt taccgttaac ggaatcgttt tccggcatcg aaccgtagat
    94861 cagatcgtaa ttgtcggaaa atacttcacc atcaggcaat aaagtgaaaa atttggatgt
    94921 gaaactaaaa atacttgtgg aagtctgcgt cgacgcatct tttgattccg tataaacgga
    94981 aacatacgat gatgctgttt cgtcataacg aagcagggtc atattgatgt tgtggacata
    95041 tttcactcct ctgatttcgt ctggagcgat attgtcgata taattacgga cataagtgat
    95101 ataatcatcc gttaaaacgt ttgatacaaa ataattggcc agacggtttt catcataggc
    95161 ggtggcatat tccgtatcgg gatatgcttc catattatct tcataagccg acatcatttt
    95221 gtcgatatcg agtctagaat cggagattgt gatgggataa ccggccaaag tttcccgttc
    95281 gaaatcattg atctgttggt ccatgccgtt ggaaagcgac aaaatcaaag caattccgat
    95341 gataccgatg ctgccggcaa aagccgtaag cagggttctt ccgagtttgg ttttgatatt
    95401 gtttaaagca gaaatgattg cagttttgta agacatggaa gtttttttaa gaaccagttc
    95461 ctgtttgaga tcactttcgg ttttgacggg attggtgtca tccgtcactt gcccgtcttt
    95521 cagtctaacg atcctggtgg aatacttttc cgccagttcg gcattgtgcg tcaccatgat
    95581 gacgagtttg tctttggcga tctccttgat gagatcaaga atttggacgc tcgtaacgga
    95641 atctaaagaa ccagtcggtt cgtcggccaa aacaacgtcc ggattgttta caagcgctct
    95701 ggcgatcgct actctctggg actggccgcc ggagagttgg ttcggacgct tgttaatatg
    95761 gtcggccaag ccgacctttt ccagcaaagc catagcccgt tcttttcttt ccttggcctt
    95821 gacgcctgaa agcgtcaggc cgagttccac gttttgaagt acggatagat gcggaatcag
    95881 attatggctc tggaaaataa aaccgatcgt attgtttcgg tacatgtccc attcggtctc
    95941 ggtaaaatct ttggtcgatt ttccgcttat tctcaaatct ccggaatcat aatggtctag
    96001 cccgccgatg atatttaaaa gcgtggtctt accgcatccg gacggaccca ggacggtgac
    96061 gaattcattt tcccggaaat cgaggctgac attgtcaagc gctttttgag taaaatcccc
    96121 tattgtatag cttttgctga tgttctgtag ttttagcatg attcattctc ctttatgaga
    96181 tacccgtttt ccaagatggc caaatgtctt tcgaagcatt cgtgcgattc cttgaccgga
    96241 cggttcataa ccagggtctg atggatcgtt tcctttaaaa tggcaaaact cgattcaacg
    96301 acttccctga tatatctttc atccttttct ttgaaagacg acgtatctat caatttgatg
    96361 aaattgtcca tcatgttatg gaaaaaccga ttggcgatat gttcgggatt ttttgccgag
    96421 tataacaaaa aattccggat gagtccgcag ttctcctgat tgtgagaagc cagaacgata
    96481 aagtcgtggg ttccgaccac catttcatgg agattacctt tcgaacgcga agcctttttg
    96541 ctaagaaaag cgacaaactg gtttctgaat tcttccagca aaagcgcaag caaatcattt
    96601 ttatcgataa aatacatgta gaaacttccc cgtggtatct ctgccccttt gacgattttg
    96661 tttatcgata cgtcctcgaa ggcggtggag gagaattcct ttctggcgga agccaggatc
    96721 ttttcctttt tctccacggg taaattggcg aatgttgttt ttggcacttg tataccaccc
    96781 cgaaaaaata actgacttaa ttctatatga cagggtgtca taagtcaagc aaaaaacatg
    96841 cttttctatc tttcttaaca taatctacaa aataaaaccg ccggtttccc ggcggcttta
    96901 caattgtttt cgcttatttt cccgcgtttc tgaagatttc gagtcctttg gttgtcaaag
    96961 gatgttcgat catttctttc aacactttga aaggaatcgt ggcaatttcg gctcccgcta
    97021 aagctgcttg ggtcacgtgt tccggatgtc tgatggacgc ggcaatgatt ttggtttgga
    97081 tatcgtagtt gtcgaatgct agtctgatat cttcaagtag ttcgtatccg gcatgatccg
    97141 atttcgtgta atcatccaac cggccgagga aaggcgacac ataggtggcg ccggccttgg
    97201 cggccattaa agcttgcggt acggaaaaaa ccaaagtcac attggtgggg atccggttag
    97261 aggataatac tttgaccgct ttaagtccct cgaaagtcat cggaatcttg atgacgatat
    97321 tcggatgaag tctcgcatac ccgaaagctt cctccaccat ttcttccgcc gaggcttcct
    97381 tgacttcggc gctgatcggt ccgttgacta aatctgttat ttctttgatg acatcaatca
    97441 gttttcggcc ttccctggca atcagtgacg gattcgtcgt gactccgcag atgattcccc
    97501 aggatgcggc ttccctgatg tcgtcgacat tagcggtatc aatgaaaaat tccatgttaa
    97561 tccctcctta cgattattat accaaaatct tatatacttt gttaagcaga tcagtctttt
    97621 gaagattggt ataagcaagg attttatcgc attgttcctt gtaggtgttt ctcagtttct
    97681 catcgcttaa aaccggcaga ttaatgcgga cattgaatag cgcgccttcg ataccggctg
    97741 ctagaagcaa aaccgccacg ccgagatcgg agatagcatt cttgtttccc attttcagaa
    97801 gcagatccaa gccggacaaa gccgatgcgg ccgctacggc ggcctggtag ggggtctcga
    97861 tggcttcttt cgttgccatc tcgatcgcat ttatgcgcat ggcttgttcc tcttcggttt
    97921 ctttcggaag cttgtaagcg gccatcactt tgttaaaagc gatggtatct ttgtcgattt
    97981 cttcctctaa ttgtcctctg atgccttcca gtctggcaaa ttcatccaga aacgcttttt
    98041 gttcgtcttc cgaaaaagcg gagaatttct ttttgccgat tgtcaggtgg ccaaccattc
    98101 taagcagaca gacgccaaga gctgaaacaa gggcgctgac ggatccgcct ccgggagccg
    98161 gagcgggact gtcgaccgcg tttaaaaatt gttttacttc attatcgatc attttcatgg
    98221 taaatcattc ctcctcggta aaagaaactt agtcgttata aacaagtttc cctttcttga
    98281 acacgtgttt cacgtgattg atcccgaaat gatacaacac ataggaaaaa ttgggagcgt
    98341 ccaataatac aagatcggca tgcatccctt tttcgatccg gccgaccagg ttgcctttgt
    98401 tcaggctgta agccggattc acagtggcgg aagccaaaat ctcttccggc gtcattttca
    98461 gtttgccggc cgccagttgc aaagccaaca gataattctc ggaaggagac gatccggggt
    98521 tgtagtcgcc ggcgatagaa acgatggctc ctttgtcaat cagtttcctt gccggggcgt
    98581 aaccctcgtt aagatagaaa gatgtcatcg gcagcaaggt cgctacagta tctgaagatg
    98641 ccaacaaatc gatatccttg tcactaatca tcatcagatg atcgagactt ctcgctttgt
    98701 atttgattcc gagcggaacc gcaccgaagg aattcatttc atccgcatgc agtctcactt
    98761 caaagccgag ttctttcgca tgtttgatga catcttcgct cgcttcgatg ccgaacactc
    98821 ctttttcgat gaagacatcg acatattcgg ccatatcttt ttcttttaca tagtccatcg
    98881 tttttttgac gatttccaga tattcctttt ccttgccctt gtattcggcc ggataagcat
    98941 gggcgcccag gaatgtcgac gtgatctcga ccggatggtt ttcattcaat atctttgcga
    99001 cttccagttg tttcagttcc gtatcgatgt caagtccgta accggacttg gcttcgatcg
    99061 atgtgactcc atatgataac atgatgtcca aggatttctt cgccttttcg tatagttcgt
    99121 cgaaggtcgc gtttctggtc gccttgacgg tgcttaaaat accgccgccc ttttgcagaa
    99181 tctcaagata agtcataccg gaaagtttca tcgcgtattc gttctccctt gatccggcat
    99241 ggaccaggtg ggtatgggaa tcgatgaagc ccggagtcat gatcattcct ttggcgtcaa
    99301 atattttagt gttttgttcc agccagaaag cgccgtcgcc ttcgcccaag gcttcgatca
    99361 gaccattttt cacggccaca aaggcgttcg agatgattct tgtcgtctct ttgtctttgt
    99421 ttgtaaaagg cgttttagtt ggtgtataca gttttccgat gtttctgatc agaagatcgg
    99481 ccatggttta tacctctaca aatatgattc gattatttta ttaatgtcga aatcgcgaaa
    99541 cattagatac tttcttgcaa gttccgttag ttctgccagg gacaattgtt tcgggtcgcg
    99601 aatatcatca tccatgtcaa gataatatgc gagactgcgg atgagcgcat cttttggcat
    99661 taagccgaca agttcggcgc ttttaacttc gatgtggtat cttttcgctt ccatcttgac
    99721 gacttcaaaa atccggtaaa tgggattctt tttgtaatca aggatattca tcgttacctg
    99781 gacatgattg tatttttcca ggaatgccgg accggcctgg atatattgga agccgccgct
    99841 tgatttacgg atcgctctcg ctatcttttc cggaacatcc atgtcggtag tttccagatc
    99901 aatgttgtaa gctatcagga attgtctggc gccgacggcg acgacaccga aagtcggatg
    99961 ctttgcggcc ggtccgaaat caggatgcca gttctgatct ttcagttttt cgtccatgcc
   100021 ttcgaattcg ccttttctga tattgggcaa ggacatccgg tcttccttcg atgcggcttc
   100081 ggcataaagg aataccggga tattatatac actggcgatg tttttgccaa gtgtcttggc
   100141 aaaatcaacg caatcattga tggtgacccc ttcgatcggt atgaacggga tgacatcaac
   100201 cgcacccatc cggggatgtt gtccggtctg gtgattgaga tcaatctctt ctttggctac
   100261 tcccaccatt tccaaaaccg ctttttgcat ggtttcggga tcgccgatca gggtgacgac
   100321 agtccggttg taatcaccgt ccggttcgca gttgatcatc ctgatgcctt gatatttatc
   100381 aaacaccgag acgatcctgt tgatcttttc cttatcacgg ccttcactga aattgggaac
   100441 acattcgacg attttcgcca tgtcattttc cgcctttcac atattctttt tgaacgatgg
   100501 aatggatata tttctcatca actagattcg gcattgtgat ataagcatta ttattgtctt
   100561 ggttaaatga agacgccgtc tccatggcgt ggtcattcct cgcccaggag cgtctggcaa
   100621 ctcccgccat gacatcaaat gtcatagcgc tcttcagaat ctcatcgact cttaaagaac
   100681 cgtctaagac catcccaaat ccaccgttga tgaccttgcc gataccgaca ccgccgccgt
   100741 tatggagcgt taccaaagac atgccccggc tggcgttgcc cagggccacc tgggttgcca
   100801 tgtccgccat gatgttggag ccgtccttga tgtttgcagt ttctctgaac ggcgaatcgg
   100861 tgccgccggt gtcatgatgg tcgcgtccca agttgacgga tactgtactc ttgccgacac
   100921 cacctttacc gctggaaaca gcgataatgc ttcgcatcgg agcattaagc tgcccctgaa
   100981 tacggcgatc taccggaacc tgagaatccc agctaatggc aatattgtcg atgccagcga
   101041 ccttaccaat aattgcatcc tgacac
//
LOCUS       DAOPJL010000002        67231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_414_length_67231_cov_10.302266, whole genome shotgun sequence.
ACCESSION   DAOPJL010000002 DAOPJL010000000
VERSION     DAOPJL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 67231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67231
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_414_length_67231_cov_10.302266"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..82
                     /locus_tag="PLP02_00460"
     tRNA            7..82
                     /locus_tag="PLP02_00460"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Phe,seq:gaa)
     gene            210..1259
                     /locus_tag="PLP02_00465"
     CDS             210..1259
                     /locus_tag="PLP02_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPF41970.1"
                     /translation="MEKKITLRHHVHDYECMWNGIEDLYIAKSGEALPESFFFLLASF
                     GAFCYLHTNSQDIKRMIMFGDGRTKKMYEYLAPIVEFQYKHYSYTSFDSLLKTVIREI
                     DDDKPVVLGAMDMFYLPYFEKIYHKEHIPFHYVLANGYDLDKKTVYVYDGARENQIGL
                     SFKTLEEALDCYSPGLSKKNTLCKIRMGETRSKYSISKLALAKKARAFLNPPTNFLGI
                     KGMEKLIDEIKCWDKELSQEDIDQILENMLMFLGSVPTLPNRLKKLNEKDQVTFKGNY
                     DKVCQMMDVLANEYADKAYLSTIPYFYACAQTMEKISDLIVDHLLMHATTSVSMPLLL
                     AEIKDNLEKAFKILL"
     gene            1467..2363
                     /locus_tag="PLP02_00470"
     CDS             1467..2363
                     /locus_tag="PLP02_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF41971.1"
                     /translation="MDILIQQYPNDSSFGETFCEVRDFLFRINEKDLSTVDFPWGRWE
                     WAFSLPFLPVDRLTKMAIWRHEKKIVAIVSYESTAWDAYFAIDPEYSFLKEEVLKHIA
                     NEMPGPDGIHILIPDKDIKAQAIAREIGFVSGTGKEYNAVIDIDSSINYVLPPGFSIT
                     SLNERFDLCEYNRVLYRGFNHPGPVSDDHEEIMSRLKSLSGPDVDLTLNTAVVAPNGH
                     FVSYCGIWHKKGSKYALVEPVATDPEYRRMGLGKAAVLAAVAKCGLQGALKAFVGSRQ
                     EFYYRLGFKPYSEATFWERPNK"
     gene            2387..3181
                     /locus_tag="PLP02_00475"
     CDS             2387..3181
                     /locus_tag="PLP02_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF41972.1"
                     /translation="MIVNQIRIKPGKETEYPFNLTIFKQEIILDVERPVTVFVGENGS
                     GKSSLLKLIQSRMRLVEIKWQDNPEEKHAIDASTVDIMPKLGKPRGFFFESLKFINYM
                     EYMQKEIAYSNAELERVDKEYKNRSPYAKMMAESPFKRTIGELDRMYSRDLPHSSHGE
                     AYLDFFASRIRDNQIYLLDEPETPLSFQNQLTLAALIIQATKKGCQFIIATHSPIIAA
                     IPEAFIYEIEENKFVRTNFDEIKDIQMLRQFLNHKEQFLRHLDEDD"
     gene            3285..3734
                     /locus_tag="PLP02_00480"
     CDS             3285..3734
                     /locus_tag="PLP02_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPF41973.1"
                     /translation="MYCKNCGAEIKEETGVCQNCGMAMLPEPVMESKATRKSSIRLSK
                     LFSMLLGIAMVSGLVCLWLSVALGVVYYVGNHYENNYYFYPDYVLAVSALAIYISTTI
                     LSAGNLVLSCLEQKGNKNPESIMLAVFILVASSLMSVAAYVMNYFSR"
     gene            3762..4193
                     /locus_tag="PLP02_00485"
     CDS             3762..4193
                     /locus_tag="PLP02_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPF41974.1"
                     /translation="MAKFCVYCGAELKEGADICLSCGKFVVDDNDKSAIKEEKKPATG
                     RLCCVSGILLTLTIVFYITAILKAWVDSGNHYFWLGNDHYLAMILAAAFFVMSIVTFV
                     IAITEYKAKKIGLNERYISIFLFTVGMIMPYVMMVDWFFQQ"
     gene            complement(4268..5008)
                     /locus_tag="PLP02_00490"
     CDS             complement(4268..5008)
                     /locus_tag="PLP02_00490"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPF41975.1"
                     /translation="MTDDKIDKFRNIVKESNSIVFFGGAGVSVPSGIPDFRSAKGIYS
                     ERLNDTYSPEEMVSHDFFTRHPDLFFNFYFSKLVYSDALPNKVHQVLAKWEKEGKLKA
                     IVTQNIDGLHQMAGSKNVMELHGSVHRNYCQSCHRFFALEELDKTGIPKCPCGGVIKP
                     DVVLYGEALDEDVIEKSVKAIAMADTLIVGGTSLMVYPAAGLIRYFHGKHLVVVNLGE
                     QSGHFRDSYDYLLIQDDIAKVFAAVEEK"
     gene            5259..5828
                     /locus_tag="PLP02_00495"
     CDS             5259..5828
                     /locus_tag="PLP02_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPF41976.1"
                     /translation="MGILEVVLLAVGLAMDATAVSLANGLCETDLKIRKSLLMAFLFG
                     AFQAFMPLVGYFAASFFAGFLSRIIPWLALIILSILGIKMITEGKKHISCDCRERHIS
                     LKRMLIQAVATSIDALAVGLLLVETQLSPMLEYVAIIGGITFLLSMIALRLGKRIAPF
                     LENKAEYVGGVILIGIGLKIFIEYLISIF"
     gene            5918..7570
                     /locus_tag="PLP02_00500"
     CDS             5918..7570
                     /locus_tag="PLP02_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HPF41977.1"
                     /translation="MKKKMLVTAILLAGLFLPMCRVLAYNPNCLPGGDNYLSSDNFVL
                     NGDQYETIAPFLVKPYTDYLFSFPEYYESVEPYINIGLYTNSDYMDELCFTYENFSYN
                     HDYRVFFYSFRTGPDINYISVTLYDGGYITTNGFNGFILEEGTTYDGYERYIEGATVD
                     TDAPYFQGTNTIVSYVDTPITVAQIQSALTAYDAIDGDLTASIVMEADGYTPNNQILG
                     TYIVSFSATDSSGNKNTVDIIVEVVDVVSPVFGDIGTIIAVYPNVYSPEDIKAMLNAS
                     DNYDGDISSYILLTSDGYSSNNQTVGSYQMEFSVSDSSGNLATHTVTVEVVDQEAPVI
                     SGISEINIGYDAVINSSYVKSELSVIDNYDPVGSMSLVLVSDTYSANSQTIGNYAMVF
                     EVYDQSGNRSEKTVLINVVDSIVPIVYFDTSVIQVYSDSVLTLIDITKILGEAKELDM
                     AFDYDVFVLYDSYSRHASVPGTYHISLRFVPSSGGVMDKNLQICVIDRTPDFHVSAIP
                     EEGQTEGFFSRNIWYILTTGVMIASGGIVLTIKLVLKKKQVV"
     gene            7668..8591
                     /locus_tag="PLP02_00505"
     CDS             7668..8591
                     /locus_tag="PLP02_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41978.1"
                     /translation="MKKILSALLVFVMVGVLSACTQKELVTVMVPSGSPALAQLYIQE
                     DTETYQVDVVQGADPLIAAFTAGTHDFIFAPTNLGAKLYTASPDYVYIAAVVFGNYYL
                     VADMGDSEDTFDLSYLDGREMTAFGANSTGDIVMKYIFDSADITPNIIYVDSVATATS
                     LLISGETDIILTAEPSLSVLLAQNPNYAVIDLQTEYENLTGESSYPQAGVFAKATLTN
                     RVINNFLKDLEDSIDQVNESPSAAATLATTLGYTFGESVLQTAIPNSHLGFVGALDSR
                     SDLEAYFTVIMSVSAPLIGGALPVDDFYYQP"
     gene            8608..9378
                     /locus_tag="PLP02_00510"
     CDS             8608..9378
                     /locus_tag="PLP02_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF41979.1"
                     /translation="MKKTYLFLLAVFTIFCIWMIGYVVVSSPLLMPDPYAVLKALVGF
                     FGKWTAVKSILMTIMRLVIAMAAALLLGGSLGIIAGFHPKFSVYMQPLVAILRTIPVI
                     SVIVIILIVFGFETAPYVITFLMIFPLIYQAFHDGIEQLDQELVDVYRLEDNNWFTGL
                     RFCFLPLIKNQITTVLLQSAGLGIKVLVMSEYLSQTKNSIGNSLYLARINLAYDEVFA
                     WTVILIIMTLSLELVINHFKNREQKVTVSSTGVKSELD"
     gene            9541..12261
                     /locus_tag="PLP02_00515"
     CDS             9541..12261
                     /locus_tag="PLP02_00515"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HPF41980.1"
                     /translation="MDAYQKNTEELLKEQNVRLKEGLSSEEANARIERFGRNELKEKK
                     KTTLFQMFLEQFKDFLVIILLGAAVVSIITAIFSGEGLVDGIAILAIVIINAILGVSQ
                     EQKANNALAALKKMSSPHAKVLRDGIVREISSVDLVPGDIVYLETGDYVAADVRLLES
                     TNLQINESSLTGESQPVEKDARVVYHDDTVLAERHNVAYMSTLVTYGKAIGIVTGTGM
                     ETEIGKIATMLEAVEEGKTPLQKTIDVFAKMLGFICIGVSVVVFVLGLLEGQKVLYIL
                     LTAIALAVAAIPEGLTAVITVVLALGMQRMVKRHAIIKNLSTVETLGQATVICSDKTG
                     TLTQNEMTVRKVYDLEKEYDVTGTGYELSGDIIHKDKKAEVTGNLDTIMKISLLCNDA
                     IMEEKKVLGDPTEGALLVLAVKGGYTIAGKEDKYPQINEYSFDSERKMMSSINKINGE
                     YYVLTKGACDQLIKKCDRVEINGEIRPITKKDVEKVLAKNQEFSEQAYRVLGYAYQKT
                     DTPEANDLEQNLIFVGLTAMIDPPRPEAKTAIEECHHAGIRVMMITGDHLATAKAIAS
                     ELGILKPGHLALSGEDTAKMTDEEFEEAILKCDVFARSTPQDKIRIVEILQKHGETVA
                     MTGDGVNDSPALKQADIGVAMGITGTEVSKEASNMILTDDNFASIVNAVEEGRIIYAN
                     IRKFTIYLISCNIGEILVILLAMVFSFLFKAIDPETGALVSVLPLSAIHLLWINLMTD
                     SFPAFALGMEKGEKGIMDEKPRSSKEKLLNKETLIKVFVQGFGLSVAALLSFWLGLNA
                     FPGADLFQARTMIFVTVVFGELFRAYSARSETKFICQSNPFANKYVNYSVFVSVGLLL
                     LLVYIKPFAEVFGIEALSGMELFVALSLGFIPMLSGEVTKFFHGKKNDNS"
     gene            12339..12875
                     /locus_tag="PLP02_00520"
     CDS             12339..12875
                     /locus_tag="PLP02_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF41981.1"
                     /translation="MRNNVSKNVTLAPGENVAKDFPKLKARRSFCQIMLTTKRLIIYS
                     NGQSMSNGRKVNRKSMNEIDLNSIHNYEYYVDRHRHSLFVRILGFFLIVAALAFAYMA
                     IIGMVTIQSYPYSYILNYVVLGLVVMLGLLMMFAGPKTLTVIITSGINNVVKLALEAN
                     HYNELAVRYIGSKIHPSY"
     gene            12999..14813
                     /locus_tag="PLP02_00525"
     CDS             12999..14813
                     /locus_tag="PLP02_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF41982.1"
                     /translation="MKLNYKKIFAVGLAFFLISMFWQAYDNIIAKILIDKFGLNQTWS
                     GAVMALDNVLALFLLPLFGALSDRKNSKLGRRTPFIIVGTVIAAFAFMGMSFVDNYQT
                     TKIQTETTIIEDYSSLKTTELTEVSDWETALSDMLAERDAALAEENITQSQYDKWKTG
                     TYDKMQAILDDATGTLDEDSIDSLNSLYYTYFSTQIWHWNDVITSMYQERFAALQDDG
                     ITREQFDSWKTKILDPMLQILIDNPDGLSSFDVNDINDLYYNYLSTRAWQLTASNPTN
                     FVVFMLVLFVALVAMSTFRSPAVALMPDVTIKPLRSKANAIINLMGTLGGLLILILLK
                     IYAMDNLSFVHYTLAFVSVGILMLIFLGIFLWKVREPKLVREKEEEERRYGLTEEEEA
                     KEVGETVEELSAEKKKSLYLILFSVFLWFIGYNAVTSKFSDYAPKVLELGYSLPLMIA
                     YATALISFIPIGIIATKIGRRKTIMIGIVILTLCFGSAAFLTKDSSWIMYIIFGLTGI
                     GWATINVNSYPMVVELSKNSNVGKYTGYYYTFSMAAQIITPIFSGFLMDKIGRIALFP
                     YGALFVASAFITMMFVKHGDAKAVKKDSVIENFDVDMD"
     gene            complement(14873..15607)
                     /locus_tag="PLP02_00530"
     CDS             complement(14873..15607)
                     /locus_tag="PLP02_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPF41983.1"
                     /translation="MMRDTEWIKTGLFAHRGLHSEDGKIPENSMAAFRLAIEKGYGLE
                     MDLNVLKDGTVVVFHDKTFKRMCGDDRKLFDVTLEEARKLRLNDTDETIPTLTEFLKM
                     VDGRVPLLIEFKYHGSIELLCAKAMEILKDYKGVWAMHSFHPQVLLWFRKNHPEILRG
                     VIAEYFLEETDMKKITKFIMKRMLLNAFTRPDFINYNLRDMPNRFLNRAKKRGLTVIG
                     YKARTMEEFAFVKKYYDNAVFEFIEP"
     gene            15731..16906
                     /locus_tag="PLP02_00535"
     CDS             15731..16906
                     /locus_tag="PLP02_00535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPF41984.1"
                     /translation="MENFRFYSPTEIVFGKQSENEVGELLKQQGATNVFIHYGGGSVV
                     RSGLLDRVIATLDENKIQHFELGGARPNPRSGLVYEGIKLCKQNKADFVLAVGSGSAI
                     DSAKAIAAGSKYDGDFWDFYEGKARIKDALPVGVILTLPAAGSEASQSAVITQEKGML
                     KKGITSRFYRPRFAIVDPELTYTAPMYQMACGVVDMMGHIMERYFTNSSGTDLTDAMS
                     EAVLRQIILAADNMIKDNKDYEARATLCFAGTIAHNGILGVGKKEDWTTHFLEHELSA
                     LYDVAHGAGLAVMYPAYMKYVMDTNIERFRKLAINVFEIKETNDSKSVALQGIEALKA
                     FYRKIGMPTNFQELGVKKEDIPLLLATLEKNRGQKFGNFQELTLDDAKNIYLLACES"
     gene            17138..18304
                     /locus_tag="PLP02_00540"
     CDS             17138..18304
                     /locus_tag="PLP02_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPF41985.1"
                     /translation="MIVGIPKEIMHGENRVSSTPETVRKMVADGHKVLVQNSAGNGAF
                     FHDPEYEAAGAVMVGDPREIYAKSDVILKVKEPQYNAALGMHEVEMMHPGQVLITFIH
                     PASPVNHEMVRNLTAAGVIGLTLDGIPRISRAQSMDALSSMSTCAGYKGIIMAADCIS
                     KFMPMIGSAVGMIKPANVLVIGTGVAGLRALATAKSLGAVVYSADIRPEANEQAKSLG
                     AKIVETGVPTDIAVDPDGKHANTLSEKWLDAERGNLKEAVSKADIVFLSALLMGKRAP
                     ILLDEEMVKSMKPGSCIVDISIDQGGNCSITTPGEREIKHGVVIEGCKNIPGMLPTSS
                     TWMFANNIYNLFKYLAPNGKFNLNEDDEIVKSILVCHEGKLVHAGTIEAMAQAN"
     gene            18311..18619
                     /locus_tag="PLP02_00545"
     CDS             18311..18619
                     /locus_tag="PLP02_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004008230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPF41986.1"
                     /translation="MTLLQAILLGVFAASTVIGYFLINNVPSLLHTPLMSGMNALSGI
                     TVLGALVATSVAISGQNPILSGFLGGLAIIASMINVVGGFTVTDRMLKMFSRKEKHHE
                     "
     gene            18612..20033
                     /locus_tag="PLP02_00550"
     CDS             18612..20033
                     /locus_tag="PLP02_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPF41987.1"
                     /translation="MNRTLALGLEAFSNTLYYVLVGVLVLAVLFGIYLMSKVEKAKTG
                     NRISAVAMALAIILTLVKNAILPVWILYVCLAVGLIIGLILAFRVKMIEMPQMIALFN
                     GFGGLASAIVGGYALFGIGSGADSFSRIVATIAFEIGIITFFGSLVAAGKLHRVLPQK
                     QITLPMHKIWLIALFAGCVFGIMLSFFSSVDPIIVAIISFAVSTGFGLIFALGVGGAD
                     MPITISLLNSLSGVAASISGLAIGDALLVAVGGIVGASGLLLTQIMCKAMNRKLFDIL
                     FGGAKHTPQVKQNVEKTPEPDKEKVEEKETLDPVSLAKTAKDVIIVPGYGMAIAQAQH
                     LVKQLADYFRDNNARVRYAIHPVAGRMPGHMNVLLAEADVDYDDLYEMDSLNDDFKTA
                     DLAVVIGANDVMNPAAREAEGTPIYGMPILNVDQCPNVFVFNYDLKPGYSGVENPLYS
                     RKDGLYLFLGNASETLKDFLNKL"
     gene            20165..21184
                     /locus_tag="PLP02_00555"
     CDS             20165..21184
                     /locus_tag="PLP02_00555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPF41988.1"
                     /translation="MKCLVTGATGHIGNVLVKNLYENHYQVTALVLPKDSVRMIEPYC
                     DIVEGNVLDRDSMFDLVKGYDVVFHLAGIVEIGSGKKKTLMAVNFMGTKNVFDACVVN
                     NIGKFVYTSSVHAIEEKPFGETITETKIFDPARVHGLYGKSKAKASDYVMTHYSGNTK
                     VIVVMPSGVIGPYDYQRSNISQMFVDILLGHLTAYVKGGYNFVDVRDVANGIRQAMEK
                     GKDHETYLLTGNEITVKELLDKTTNYIGRKPIKTKVAAWFLLAVSYLAEFYYIVLRQK
                     PLFTHYSIVVLRSNYAFSNEKAKQELGYTVRGIDESIKDSIDFVKNNFVEKKGKKFKK
                     KIIAN"
     gene            21271..23613
                     /locus_tag="PLP02_00560"
     CDS             21271..23613
                     /locus_tag="PLP02_00560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPF41989.1"
                     /translation="MKEIRASVKDIAEYLYGGGDLANDRLMAIRQQEGISIHQYWQSL
                     YKENDQKEVYVASEDKTGDIFLIVSGRIDGIVTRDEELMLEEIKSTRQEPAAIDETTY
                     PAHLAQAKIYAHLYARENKLKRLTVVLTYVNVDTKESNQIEKNYSAKILENYYNKAIQ
                     KYIEWVMLLEEHEQKRAKSIEGLNFPFDSYRPRQRDMMASVYRNILEKGTLYLTAPTG
                     IGKTIGTIFPALKAINEPRQKVFYLTAKNDGKQIALDTVSLLETSGLHAKTCELTAKD
                     RMCFLKERDCDPENCKYARGYYKKIYKAIRDAYSNATLFTSGVIKSFARKHVVCPFEM
                     SLDLSNYCDIVICDYNYVFDPRVRLMRYFEERSADPILLIDEAHNLVQRSREMYSATV
                     TWQMFRDLEKLCQNVKPSPKREISRIVDLFQELELELIEVDFVKYEEQNEYLIQLLKK
                     LLLRLDQILFGEKKVPNRSQIIEAYFSISQFVRISEFYNQEFVFLLEKQNDDVFVSIK
                     CLNASGFINKTIGEYALSALFFSATLEPIDYYKTLLTEGKGNDSEYPSPFKKDNLLVL
                     IGEHVSTRYQDRKDSVSEIALATKALISGKKGNYIVFFPSYEYLKMVEERLDFDSSST
                     DIIIQTRQMSQEERSSMVMLFKTRSEKTQVGLFVMGGVFGESIDLIGDMLSGVLVVGV
                     GLPALSPYNNILRSHFDLEFGQGFDFAYTYPGLNKVIQAVGRVIRTETDRGVAILMDD
                     RFASNRYIKLYPRQWDKAEICGNTKEIEERIARFWQKKEK"
     gene            23819..24127
                     /gene="rpsJ"
                     /locus_tag="PLP02_00565"
     CDS             23819..24127
                     /gene="rpsJ"
                     /locus_tag="PLP02_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPF41990.1"
                     /translation="MAANQVIRIRLKSYDHRLLDQSARKIVDTAKKTGAVVSGPIPLP
                     TEKEIFTILRSPHVNKDSREQFERRTHKRLIDIINPSAKTIDSLMHLDLPSGVDIVLK
                     "
     gene            24154..24786
                     /gene="rplC"
                     /locus_tag="PLP02_00570"
     CDS             24154..24786
                     /gene="rplC"
                     /locus_tag="PLP02_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPF41991.1"
                     /translation="MAKGILGRKVGMTQVFDENGHLVPVTVVVVTPNVVLQKKTVATD
                     GYEAVQLGFMEKRANLVNKPLTGHFAKVNTTPKRYVKEIRLPEMMEYEVGQEVKCDIF
                     APGDYVDVTGTSKGKGYQGVIVRHNFSTGPMKHGSKYHRGVGSMGSIAANRILKGKAM
                     PGRMGHETVTIQNLQIVRADVENSILLVKGSVPGPDKSLVIVKNATKKQK"
     gene            24814..25437
                     /gene="rplD"
                     /locus_tag="PLP02_00575"
     CDS             24814..25437
                     /gene="rplD"
                     /locus_tag="PLP02_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010024545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPF41992.1"
                     /translation="MPTIKLMNQAGEAVGNIDLSEAVFGAEPNQQITYEVVKAQRAAM
                     RQGNASTKNRSMVRGGGRKPYRQKGTGHARQGTIRAPQFVGGGTVFGPSPRSYSYKVN
                     KKIRRQALRISLSDKVREESLVVVDKITLESFKTKGVVEILANLKLEGKIMVVLDTID
                     ETFDIASRNLPNVIAFASDHISVYDVMNSAKVLATEAAIKKIEEVLG"
     gene            25437..25718
                     /gene="rplW"
                     /locus_tag="PLP02_00580"
     CDS             25437..25718
                     /gene="rplW"
                     /locus_tag="PLP02_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020006867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPF41993.1"
                     /translation="MDKYQIIKSPVVSEKSTKLAEQRKYTFEVDKNANKIQIKQAIEE
                     IFDVKVTKVNVVNCIPKTKKVGKFEGLKPAVSKAVVTLAEGYKIDIYTA"
     gene            25735..26565
                     /gene="rplB"
                     /locus_tag="PLP02_00585"
     CDS             25735..26565
                     /gene="rplB"
                     /locus_tag="PLP02_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPF41994.1"
                     /translation="MAIIKFKPQTNGQRNKTKLDYAEITTSKPEKSLTVSLKKEAGRN
                     NQGKITVRHQAGGVRQKYRIIDYKRNKDGIPGKVATIEYDPNRSANIALINYADGEKR
                     YILAPKGLEVGSTIVSGKSVDIVTGNAMEIMSIPVGTVIHNIELYPGRGGQLIRAAGT
                     SAQILGREEKYVLIRLKSGEVRRILGVCRATIGEVGNEFHELVKLGKAGRKRHMGIKP
                     TVRGSVMNPVDHPHGGGEGRQPIGHPSPLSPWGKPTLGKKTRKNNKASDKLIVRRRNK
                     "
     gene            26583..26873
                     /gene="rpsS"
                     /locus_tag="PLP02_00590"
     CDS             26583..26873
                     /gene="rpsS"
                     /locus_tag="PLP02_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPF41995.1"
                     /translation="MSRSIKKGPFCDDHLMKKVTEANKSNSKKVIQTWSRRSTIFPQF
                     VGLTIGVHNGREHIPVYITEDMVGHKLGEFSPTRTFRGHADKKAVKKSGGAQ"
     gene            26873..27217
                     /gene="rplV"
                     /locus_tag="PLP02_00595"
     CDS             26873..27217
                     /gene="rplV"
                     /locus_tag="PLP02_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPF41996.1"
                     /translation="MEAKEAKAVLRSVRIAPRKVRLVVDLVRGQSINDAYAILRNLQR
                     GASKPVEKLIKSAAANADHNFQLDPEKLYIKEIFVGEGKTFKRIQPRSRGRAFAILKR
                     TSHIYVTVAERE"
     gene            27228..27875
                     /gene="rpsC"
                     /locus_tag="PLP02_00600"
     CDS             27228..27875
                     /gene="rpsC"
                     /locus_tag="PLP02_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPF41997.1"
                     /translation="MGQKVSPIGQRIGIILDWESRWYADKRDVADLLIEDMKIRELLN
                     ELYKNASVSKIVIERSKSRVIITVNTAKPGMVIGRNGETKNAVVDKLKKLTGKEVFLN
                     VVEIKRAELDAKLVADSIARQLENRASFRRVQKVAIQRALKAGAKGCRTLVSGRLAGA
                     EIARAEGYSEGNVPLHTLRADIDYALAEAKTTYGKLGVKVWIYKGEKLPQKKEAR"
     gene            27879..28310
                     /gene="rplP"
                     /locus_tag="PLP02_00605"
     CDS             27879..28310
                     /gene="rplP"
                     /locus_tag="PLP02_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPF41998.1"
                     /translation="MLMPKRTKYRRPHRVRYEGHAKGGTEITFGEYGLMALEGAWISA
                     RQIEASRVAMTRFMKRAGQVWVKIFPHMAKTKKPLEVRMGSGKGAPEDWVAVVKEGLI
                     MFEIAGVPEDVAKEALRLASHKLPIKTKFVKKECGDENGTC"
     gene            28297..28491
                     /gene="rpmC"
                     /locus_tag="PLP02_00610"
     CDS             28297..28491
                     /gene="rpmC"
                     /locus_tag="PLP02_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPF41999.1"
                     /translation="MAHVSQIREMETDAILKEIDNLKKEMFDLRFKQATGQLENTARL
                     HVVRKTIAQMKTIISERERE"
     gene            28503..28763
                     /gene="rpsQ"
                     /locus_tag="PLP02_00615"
     CDS             28503..28763
                     /gene="rpsQ"
                     /locus_tag="PLP02_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPF42000.1"
                     /translation="MEEKKLHRVFTGTVVSAKNDKTITILVTSYKKHPLYNKRVISSK
                     KFTAHDEKNEAKEGDVVRITECRPLSRTKKFRLIEIVEHKAE"
     gene            28788..29156
                     /gene="rplN"
                     /locus_tag="PLP02_00620"
     CDS             28788..29156
                     /gene="rplN"
                     /locus_tag="PLP02_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPF42001.1"
                     /translation="MIQQETRLKIADNSGAKEILTIKVLGGTSRKYANIGDIIVATVK
                     SATPGGLVKKGEIVKAVIVRTKRGLQRPDGTHIKFDDNAAVIIKDDKSPKGTRIFGPV
                     ARELRDAEFTKIVSLAPEVL"
     gene            29172..29513
                     /gene="rplX"
                     /locus_tag="PLP02_00625"
     CDS             29172..29513
                     /gene="rplX"
                     /locus_tag="PLP02_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPF42002.1"
                     /translation="MKFKKGDKVVIIAGQDKGKKGTISKILTKSERVTLEGEGLAKVK
                     KHLKPSQIAPDGGIMDVDKPIHVSNIMLLDPKEKKRTRIGYKEVTRKTKKQQDETETV
                     RYAKKSGEVLE"
     gene            29530..30072
                     /gene="rplE"
                     /locus_tag="PLP02_00630"
     CDS             29530..30072
                     /gene="rplE"
                     /locus_tag="PLP02_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPF42003.1"
                     /translation="MNRLQEKYKSEILPALTEKFKYSSVMQCPKVSKITLNIGAGDAV
                     QNPKVLDDAVAELTQITGQKPIITKAKKSIANFKLREGMPIGCKVTLRGERMYDFLDK
                     LITIALPRVRDFRGINPRGFDGRGNYTLGIREQLIFPEINYDKVSKIRGMDIVIVTTA
                     KTDQESIELLKMFGMPFRKN"
     gene            30085..30270
                     /locus_tag="PLP02_00635"
     CDS             30085..30270
                     /locus_tag="PLP02_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPF42004.1"
                     /translation="MAKTSMKVRQQRGSKFPVRNYTRCERCGRPHAVYRKFKLCRVCF
                     RELAYKGQIPGVKKASW"
     gene            30323..30721
                     /gene="rpsH"
                     /locus_tag="PLP02_00640"
     CDS             30323..30721
                     /gene="rpsH"
                     /locus_tag="PLP02_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPF42005.1"
                     /translation="MVMTDPIADMLTRIRNANQMKHKTVDVPASKLKAEILNVLKNEG
                     YITDYEFVGGSVQGNLKVTLKYLPNDARAVRGLKKISKPGLRVYAKSDDLPRVLNGLG
                     IAIVSTSQGIMTDREARRKQVGGEVLAYVW"
     gene            30761..31303
                     /gene="rplF"
                     /locus_tag="PLP02_00645"
     CDS             30761..31303
                     /gene="rplF"
                     /locus_tag="PLP02_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014930777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPF42006.1"
                     /translation="MSRIGKLPIVIPAGVSITVDKDNHVTVKGPKGVLEFTFSKDISI
                     ATENNEITVTRPSDSLIHRELHGTTRAVLNNMVVGVTEGYTKILDIKGVGYRAALKGS
                     NILVLSVGYSNPVEIAIPENLTIEVPSNTEIFIKGIDKQAVGEFAAVIRKVRAPEPYQ
                     GKGIRYRGEHIRRKEGKTAK"
     gene            31322..31678
                     /gene="rplR"
                     /locus_tag="PLP02_00650"
     CDS             31322..31678
                     /gene="rplR"
                     /locus_tag="PLP02_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPF42007.1"
                     /translation="MFKPVNKNVQREKRHWRIRTIIKGTASRPRLNVYRSNKAIYCQI
                     IDDDLQTTLLAASSHEVKDVNGGNVEGAKAVGTLIAKKALEKGIKEVVFDRGGYLYHG
                     RVKALADAARAAGLEF"
     gene            31696..32289
                     /gene="rpsE"
                     /locus_tag="PLP02_00655"
     CDS             31696..32289
                     /gene="rpsE"
                     /locus_tag="PLP02_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPF42008.1"
                     /translation="MAENIEKDGNKERESRDNRGRRPSNRRDNRGGQQRKKEEKLYEE
                     RVVNIGRVTKVVKGGRRFSFSALVVIGDRKGKVGFGTGKASEVPDAIQKAIEDAKKNM
                     FVIPINGSTIYHTVTGTYGAAKVFLRPAVAGTGVIAGGAVRAVLELAGVEDVLSKSLG
                     STSPINIVRATMDGLLSTRSIQEVAETRGLTPEEVLK"
     gene            32289..32471
                     /gene="rpmD"
                     /locus_tag="PLP02_00660"
     CDS             32289..32471
                     /gene="rpmD"
                     /locus_tag="PLP02_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPF42009.1"
                     /translation="MAKIKITLVKGLVGRKPNQVKTVKALGLKKRNSSVVKEDNDAIR
                     GMINTVAHLVKTEEAK"
     gene            32484..32924
                     /gene="rplO"
                     /locus_tag="PLP02_00665"
     CDS             32484..32924
                     /gene="rplO"
                     /locus_tag="PLP02_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006093108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPF42010.1"
                     /translation="MKLNELKPVQGATHYSKRLGRGTSSGTGKTAGRGQKGQNSRSGG
                     GTRLGFEGGQTPLYKRLPKRGFTNFFRKEYAIVNLATLNKYKNKSVVTPEKLLEDGIL
                     SDLKDGVKILANGKLEKSLTVKANKFSAAAEEAIKAAGGKTEVI"
     gene            32935..34284
                     /gene="secY"
                     /locus_tag="PLP02_00670"
     CDS             32935..34284
                     /gene="secY"
                     /locus_tag="PLP02_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPF42011.1"
                     /translation="MKKIFKNKDILKRIGFTLLVFFVFKLLTYVTMPLVDKSALSSLF
                     ENSGFLGIINSISGDALQNYSIVALGITPYITASIIVQLLQMDIIPALKEWSEEGEVG
                     RQKTARLTRYLAIGLAFVQAVAMTFAFNSATTQTTIFKPGVVAWASEDNRNLWFLVYF
                     YIAIVLTAGTAFMIWLADQITLRGVGNGSSMLIVAGIIISFPGTIVSLIQYYITDPSG
                     AMTTVSASQGTLVGYILFAVCILMFVIVLIGITYMQDATRKVPIQYANRPAGSKFSGK
                     SESNIPIKINTANVIPVIFAAIILSLPNTIFNYITLGDTASNWMGEIFSYNQPIGFAI
                     YIILIFIFTFFYTFILIKPEQTAENLQKQNAYIPGVRPGTETEAYITKTLFRVTVVGA
                     LYLVMIASLPIITSMALGHSYAQIGGTSLLIIVGVALETAKQIETDTKDKAYAGFLR"
     gene            34295..34948
                     /locus_tag="PLP02_00675"
     CDS             34295..34948
                     /locus_tag="PLP02_00675"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPF42012.1"
                     /translation="MTNLLIMGKPGAGKGTLAAVILENYHLKHISTGDIYREEMQKES
                     PIGLLAKSYINGGKLVPDDITNEIVENVLKNTKEYPNGFMLDGYPRTISQAGTLDKML
                     TDLNLKLDLVVNVDISEPVLMRRITGRRICSVCQETYHIENKPPKVPGICDVCGGTLI
                     QRKDDTEEAVATRLAVYNEKTKPLLEYYQDKEMLFTVNGELPAEVIFKDIVKRLEAV"
     gene            34949..35701
                     /gene="map"
                     /locus_tag="PLP02_00680"
     CDS             34949..35701
                     /gene="map"
                     /locus_tag="PLP02_00680"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPF42013.1"
                     /translation="MITIKSEREVELMREAGKIVALAHKAAKEAIAPGVSTKKIDEII
                     EKVIRDHDATPSFKNYNGFPAASCISVNEHVVHGIPSREMILKDGDIVSVDIGACYKG
                     YHGDSAWTYPCGHVSERVMNLLEGTEKSLFAGLSQAKAGNRLTDISNAVQKTAEGLGY
                     SVVREFVGHGVGQKLHEDPAIPNFGHPGRGPLLRQGMTLAIEPMINLGRKEVRILPDG
                     WTTITADGKPSAHFEHTVLIKEDGYEILTKVN"
     gene            35715..35936
                     /gene="infA"
                     /locus_tag="PLP02_00685"
     CDS             35715..35936
                     /gene="infA"
                     /locus_tag="PLP02_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPF42014.1"
                     /translation="MAKKDDVIEMIGTVVEVLPNAQFIIELEIGHRIIGHVSGKIRMH
                     YIRILPGDKVKVELSTYDLTRGRITYRMH"
     gene            35955..36071
                     /gene="rpmJ"
                     /locus_tag="PLP02_00690"
     CDS             35955..36071
                     /gene="rpmJ"
                     /locus_tag="PLP02_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPF42015.1"
                     /translation="MKVRPSVKKICEKCKIIKRHGNVMVICENPKHKQRQGK"
     gene            36081..36446
                     /gene="rpsM"
                     /locus_tag="PLP02_00695"
     CDS             36081..36446
                     /gene="rpsM"
                     /locus_tag="PLP02_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPF42016.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGKTTSEKILAAAGVSIDKR
                     VKDLTDDEVVRIRQEVQKHKVEGDLRREVALNIKRLMEIGCYRGIRHRKGLPVRGQNT
                     RNNARTRKGPRKTVANKKK"
     gene            36462..36848
                     /gene="rpsK"
                     /locus_tag="PLP02_00700"
     CDS             36462..36848
                     /gene="rpsK"
                     /locus_tag="PLP02_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPF42017.1"
                     /translation="MARNVTKKRRVKKNIPVGVAHIHSTFNNTIITITDPTGNAIAWS
                     SAGAIGYKGSRKSTPFAAGLASEAAAKAAMENGVQKVEVSVKGPGPGREAAIRSLQVA
                     GLEITAIRDVTPVPHNGCRPPKRPRG"
     gene            36864..37814
                     /locus_tag="PLP02_00705"
     CDS             36864..37814
                     /locus_tag="PLP02_00705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPF42018.1"
                     /translation="MKELKFEKPKTTIEEINDTYGRFVVTPLDRGFGITIGNSLRRVL
                     LSSLPGAAIINVQIEGVQHEFTAIENVTEDVTTIILNLKGVVLTIDSSNPNVEKRMEV
                     IVDGPTDVFAKDIIADDEVAVINPDHYICHVNKGARFRMVLHARKGNGYISADKNAVY
                     DNMVGVIPIDSLYTPVVKANYIVDKTRVDDSADHDKLTVEVWTNGSISPKEAIGMAAK
                     MLIDHFYSLVELSSKAMSEDFMIERKSEEASRNLEKPIEDLDLSVRSYNCLKRAGIHT
                     LGELVEKTEEDMMKVRNLGKKSLKEVKQKLEELNLSLAKH"
     gene            37831..38190
                     /gene="rplQ"
                     /locus_tag="PLP02_00710"
     CDS             37831..38190
                     /gene="rplQ"
                     /locus_tag="PLP02_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPF42019.1"
                     /translation="MARGYSKLHRRSDNRKALISDLATQLILHGKIETSLVKAKEARR
                     TVEKLITYAKKGDLHSLRLAEARLRDLSIDNQSALQKLFKELGPKYMDRQGGYTRIYR
                     TGERLGDACPMAIIELV"
     gene            38304..39029
                     /locus_tag="PLP02_00715"
     CDS             38304..39029
                     /locus_tag="PLP02_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPF42020.1"
                     /translation="METAKVDIFMDTFRSLESYLRVEYDRGNYREQTFMNTLFRIRGR
                     QENHIIQNPQYFDILQQAAQLRNVIAHNNDVAEPTDTFLKKFQRITRMIVSPVRVSNV
                     MIPMSRIKKASTKDKIADIMDTMDRYGYSKIPVFSGQRLLGVFTKNTFFYKLLTDKPA
                     SINRDMRMENFLDVIDFDGDPTKYFDFIRKDADIFEALTRFRRNFREKNHLEMLFVTE
                     NGKKEETILGIVTLPDLEESVVI"
     gene            39052..39855
                     /locus_tag="PLP02_00720"
     CDS             39052..39855
                     /locus_tag="PLP02_00720"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPF42021.1"
                     /translation="MSFLETKDLSFSYENDKPALDKINLKVEKGEWIAILGPNGSGKS
                     TLAKAVVGLLETEKGTVFVDGNELNEKTVYEARQKIGIVFQNPDNQFVGVTVRDDIAF
                     GMENLCLPREEILKRIDFFANKVGITDLLDKEPANLSGGQKQRVAITGILAMKTQVMI
                     FDEATSMLDPYARADLMEYIRSLHNEGITIIMITHDIEEALWAERLVVMKEGRIVSDG
                     KKEDLLKSRDMLAGAFLELPVALEISNLLGDDEEAKAVKTFLWELSSKR"
     gene            39831..40691
                     /locus_tag="PLP02_00725"
     CDS             39831..40691
                     /locus_tag="PLP02_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPF42022.1"
                     /translation="MGIEFKKVSYRYMKGNAAFFQAINNISLEIDNLDEFICLVGQTG
                     SGKSTLVQHMNALIFPSEGEVVIDGVVITSKRDKKIDYNSLRKHVGLVFQFPEYQLFE
                     ETVEKDVMFGPLNFKMTKEEAAKAAKVALEQVGLDDTYLERSPLNLSGGEKKRVSIAG
                     ILALNPDVLVLDEPTSGLDPAGKRRLMELLSSIKKETGKTIVLITHDMDLVYQYASRV
                     LVLNTGNLVFDGTPDKLFRRDDLVKNHLDYPGTIKTLKAVREKFGVDIDIYKKTPEEA
                     AREIRRVMEK"
     gene            40688..41656
                     /locus_tag="PLP02_00730"
     CDS             40688..41656
                     /locus_tag="PLP02_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPF42023.1"
                     /translation="MKGIVIGQYIPGTGFFYKLDPRTKIAAVVILMVAVFLLETIPQL
                     LIALGLSIVLLLWGKISVGRVLRGLKPLLVLMIFTFFFQVLFNTEGTVIWQTTFYFSA
                     SSMIALGILTFMWRIIAKNISGFKFIVFVLYLALAILNFHYMPSWYQFGSWDFIIYSK
                     GLEMSVFVVVRLLIIITFSTVLTLTTKPTDLTQGLEKMMRPLKHIGINSDEFALIISI
                     SLRFIPTIFDEAMKIMAAQASRGADFSQGKLKDKVKQIVSLLVPMFIIAIKRSEDLAD
                     AMESRNFVPGKPRTRIYELAYKRLDLFAGLFIVLCLAGSIVLKVLI"
     gene            41653..42636
                     /gene="truA"
                     /locus_tag="PLP02_00735"
     CDS             41653..42636
                     /gene="truA"
                     /locus_tag="PLP02_00735"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPF42024.1"
                     /translation="MKQIMGTADFLAMISPDAGIKIGHEIQLGRENVQKTILANEDIF
                     DRIAFHQPFAHVAMYGEDQTGHLVKIHLQDGMIKRILVTPGITARKIKLTVAYDGTEF
                     YGFQFQKKERTIQGELEKAISEMNGNNTTCHGASRTDSGVHAFGQVVDFETDRDFTEK
                     RWLSSLNKILPKDIMIRQAEFAPKLFHSRYDVWKKEYRYSLNIGDADPKFRHTEWPIG
                     PLKDMEKLADNLKMLVGTHDFTSFATGEKEDMVRTIYETNLVIRDNKVEIDILGDGFL
                     HHMVRLIVCQLVEIANGRSDTSIREIIMEKSRKHTVRMAPPNGLCLYNIEY"
     gene            complement(42695..43591)
                     /locus_tag="PLP02_00740"
     CDS             complement(42695..43591)
                     /locus_tag="PLP02_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPF42025.1"
                     /translation="MMYTLSFFTDLFNYDFMLKALAAGVILGVIAPLIGSIVVVRRLS
                     FIADTLGHFSLVGVSFSLFLSTIGISIFFDEPLFFGLIFSIVGGLLIEMFRRSYKSYK
                     EISMVIVISLGTALSAVFFSKAKATGSLYTYLFGSILTVTNYYMYALLAITAVVAVLF
                     LVFGRQIIAVSFDETSAKFLGINVNIFQILFMVVLSIVVSVMLEAAGVLLISSMMIIP
                     VAAGMKLGWSYRSTLGIAVLFSELSVIFGLWAAYSLKIPSGATIVSINIIILLVVALI
                     KKIVLKRQIAKLAKENPVETNW"
     gene            complement(43588..44256)
                     /locus_tag="PLP02_00745"
     CDS             complement(43588..44256)
                     /locus_tag="PLP02_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPF42026.1"
                     /translation="MQIDIHNLTFAYGHRSVISGLSLTINPGDYLVVRGKNGSGKTTL
                     IKCLLGYNPVKTGMIFFDHEDINTFTGWTRFGYVNQKFEDFNYDFPITVNEMLSVYSL
                     KETGQSFRLKLLDQLGILGIVNENVNNLSGGQLQRVFIARAMLNQPDVLILDEPTASI
                     DKMNTAYFYQTVNMLNQQGITIILVTHNSDLKPLNFTHVLTMYQDMSYDFQTSEKITI
                     EEAE"
     gene            44371..44994
                     /gene="tmk"
                     /locus_tag="PLP02_00750"
     CDS             44371..44994
                     /gene="tmk"
                     /locus_tag="PLP02_00750"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPF42027.1"
                     /translation="MKGLFITFEGTEGSGKTSVIRKVGEYFDKLGYPVFVTREPGGSR
                     ISELIRNIILNKEHTEMDPRTEALLFAASRRQHLVERILPALEAHKIVLCDRFVDSSL
                     IYQGVARNLGIDEVMSINQFAIENHFPDRTIYIDVRPEVGLSRVFGTPNREVNRLDLE
                     QVAFHKKIYEGYHELMKKYPDRIRSINGEADIDTVTKAAIDQIAELL"
     gene            44999..46036
                     /locus_tag="PLP02_00755"
     CDS             44999..46036
                     /locus_tag="PLP02_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HPF42028.1"
                     /translation="MMRKMLVTWDELAGYQPYVARILGNSLKNRRLAHSYILEGPLGS
                     KRLDTAILLAKTLLCQNLQANGNPCQACHNCHRIDDMAHPNLFYVEAESEQIRKKQVL
                     ELLQEFSRQSVEKGPRIYIVNEAEKFNQEAANTLLKTMEEPGQDLYQILITSQVNALL
                     KTIVSRSQVLHFTPINKKQIVLELEKENLSQAMVAGISEYTDNTDKAREMAASQQTSD
                     ILELMFEIFKRLETGEKSAVMAFKEQRDAILPDPEAIDFFLTMMVLFLKDMLSHKIHY
                     HSLIVFESEKELIGKLADKTDQEKIEAAIDQVLDLKSTTKYNINSQLAFDALLVDLER
                     GFDHGISGSAG"
     gene            46011..46871
                     /locus_tag="PLP02_00760"
     CDS             46011..46871
                     /locus_tag="PLP02_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010309171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HPF42029.1"
                     /translation="MEYQVVPVKFNRLGKQYYFSTGGKTVNKGDKVVVETVRGIELGE
                     AASDVKMVSEQEVVLPLKPIIRVALEADIKQDETNKADEIFVMNKASELVKKNELEMK
                     LLNCEYTLDRSKLIIYFNADGRVDFRELVKDLANEFRLRIELRQVGARDGAKVLGGIG
                     PCGLPTCCSSFLGEFDPVSIKMAKNQNLSLNPSNISGLCGKLLCCIHYEDDNYREYRK
                     IAPKINSFVKTPDGRGKVTQLNFISMEARVDFDDGSSRYYKVSELEFREKATEENIDN
                     NGNDLKDLEE"
     gene            46868..47623
                     /locus_tag="PLP02_00765"
     CDS             46868..47623
                     /locus_tag="PLP02_00765"
                     /inference="COORDINATES: protein motif:HMM:NF017755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPF42030.1"
                     /translation="MNDVFLKDGEKTANLMAYEGLKIVESEKAFRMSIEAVLLANFVS
                     LNPRIARIMDFGTGTGAIPLMLSQWTKAKITGVEIQSELCELAVRSVKLNGLAQQIEI
                     DMDDIAAVHLHYRPSSFDIVVSNPPFFTGEGNKGNNDKILVAKHETQLSFSTLAKQAS
                     RMLKEGGKFDFIHRADRLQEIILVLQTEKFAIKRIRLVYSKPGTPALWILIEAANKGN
                     RGSLSFAEPLYVYDQNGNYTPEVSGYFHRPREV"
     gene            47626..48480
                     /gene="rsmI"
                     /locus_tag="PLP02_00770"
     CDS             47626..48480
                     /gene="rsmI"
                     /locus_tag="PLP02_00770"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPF42031.1"
                     /translation="MYRHSNYQPGKATLYLIPTPIGNLEDITLRALRTLKEVNAIYAE
                     DTRNTMKLLNHYKITGELKSLHEHNEKEVTPDIIERLARGENIGIVSDAGMPLFSDPG
                     YGLVQKAMGAGYNVVCLPGANAATTGLLMSGINPHPFTFVGFLDTRKSKRKARLEELR
                     FRKETLIFYEAPHRIKAMLADLAETYPDRMVCVCREISKKFEEIVKGTAEELSQIEDW
                     KGEMVVVMEGAYDDDSTVDKDSYCQQVEALVKAGTRLSEAAKKVSETTGVSKNVIYQE
                     YLAKTKKQ"
     gene            48521..49078
                     /locus_tag="PLP02_00775"
     CDS             48521..49078
                     /locus_tag="PLP02_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPF42032.1"
                     /translation="MRIACLLADGFEEIEAIGTIDLLRRCDVIIDLVGYQNREFVTGA
                     HGIVIKTETPYEKLDPAFYDGLLIPGGGKQSATLRSDTSVLEMARSFYGNDKWLMAIC
                     AGPTVFGAAGLLKNIRYTSFPGTEVYIQEGIRENRAAVVDGKIITGAGAGAVFEFAFA
                     IIEALFGTEKKIEMQNRTVFRSYER"
     gene            49083..51032
                     /gene="metG"
                     /locus_tag="PLP02_00780"
     CDS             49083..51032
                     /gene="metG"
                     /locus_tag="PLP02_00780"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPF42033.1"
                     /translation="MDKKTFYITTPIYYTSGKFHIGTAYTTCLCDTMKRYKSLMGYDT
                     RFLTGTDEHGMKIAERAKESGITPQEYVDRIAGMAKDTWKLLDIHNDDFIRTTEKRHE
                     TIVADIFEQLLAKDDIYLGEYSGNYCMECEAYFTPTQLLEGGLCPDCGRPTTIVKEET
                     YFLRLSKYQDRLLKYIDEHPDFIFPETRKNEVVQFVKTGLQDLSVSRTAFEWGIKVKS
                     NPKHVIYVWIDALTNYITALGYGSKDDSLYKKYWENGDEVVHVVGKDILRFHAVYWPI
                     MLMALDLPIKFRLFVHGWYMMKDGKMSKSKGNVIYPDQLTGRYGLDSFRYYILRELPY
                     GNDGLFTPEDFISRVNTELANDYGNLVSRSVTMVNKYFDGLVRNTKHNRSEYLGFEKE
                     LESIAKEAVNEYRQNMDAFRIPNALAEVTRLVNRANKLIDETSPWNLAKNDEDLPVLE
                     SVLYHLLEALRVASILYIPFMDNATEKVFDMLGADEDKRDFSTLMFGIQEKYIVTNEP
                     THVFPRIDAEKETEVIRTMMSVKNIDEKKVESKPEIEIDLFDKVEIKVGLVKEAAIHK
                     NAKKLLVLQINTGDKSRQVVSGIAEYYAPEELIGKKLLVVTNLKPVKLRGEESQGMIL
                     CGETSEGKLYVIEANPNLNPGDGVR"
     gene            51035..51922
                     /locus_tag="PLP02_00785"
     CDS             51035..51922
                     /locus_tag="PLP02_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPF42034.1"
                     /translation="MKENFLKNFDGKKEARTMFNTVVGTAIYALGILFFIDPAKLYSG
                     GVTGLSQLIVNVVELLSSNNIHINLGILSFILQVPLLVFAYFKLNGRFVFYTIVSVVV
                     FTLILLFRVNEPLMGSDMLTNALVGGILGGIGNGMLHRVGASSGGTSIPFQYWSIKSG
                     KSMGTYQIFLHGLIILLSGVIFGLNIAIYTMISQLISSIVLDKIFTGYNFMKLEVVTT
                     NGKEMAEALKTRLPHGVTMIDAVGAYTYQAKTVIYAVISVHEIHKYVSLIRQIDPQAF
                     VVMTGVSSVMGKFTKKIIN"
     gene            52030..52464
                     /gene="rplM"
                     /locus_tag="PLP02_00790"
     CDS             52030..52464
                     /gene="rplM"
                     /locus_tag="PLP02_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPF42035.1"
                     /translation="MKTYMANQQTIERKWFVVDAAGKVLGRLATEVASVLRGKHKPTF
                     TPNVDCGDYVIVVNADKIELTGAKWDDKLYHTHSHYPGGLKTKTAKVMMAEKPTRIVE
                     LAVKGMLPKGKLGNQMYKKLFVYAGPEHKHEAQKPVVMELKG"
     gene            52493..52873
                     /gene="rpsI"
                     /locus_tag="PLP02_00795"
     CDS             52493..52873
                     /gene="rpsI"
                     /locus_tag="PLP02_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002009728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPF42036.1"
                     /translation="MYRGTGRRKSAVARVILRPGKGELIINGRGFADYLPSSLARLDV
                     MQPLVLTANENTFDISVNVNGGGLIGQSGAIRLGITRALLEVNPEYRKILKPAGLITR
                     DPRAKERKKYGLKGARRAPQFSKR"
     gene            52994..54778
                     /locus_tag="PLP02_00800"
     CDS             52994..54778
                     /locus_tag="PLP02_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42037.1"
                     /translation="MLFGKHVNKFYWRYGVFFLFGIAALFVVDYTQLEIPNIIGYIIE
                     KLQNGTLDRITLSGYIVSLGIIGAIIVAGRFIWRIFIFGTSRRVDYDLRNEMFAHAEK
                     LSQQYYQEKKSGGLMALFTNDLNAVRMAFGPGIMIFFDVLALGGMAFYKMYLLDPILT
                     LVALIPSLMIAVLATFVSRVTGRKFRDRQKAYENLSDFSQENFYGIAVVKAFANELRE
                     TRAFASINKDNYDKNLSFIRVSTLLSVSIEMFINIVRVLLIGIGGYLVYKTSPIDPSQ
                     SFTFTIGDLTRYLAYFGALVWPMMAIGRFINLRAQARASLKRIGDFLDSPVDVTDDFE
                     TTPVEKIDGAISFKNLTFTYPGVKEPILKNINLEIKAGDTIGIIGPTGSGKTTIADLL
                     LRIYNVGPGMILIDGRDIMTLPVKQVRDTIGYVPQDNFIFSDAIENNISFSQDTIDHK
                     LVEQMARFSDIHDNIAEFPDQYLTMVGERGVTLSGGQKQRISIARALLKKPKILIMDD
                     SFSAVDTGTEEAILENLDKIRSNQTMIIIAHRVSTIKNSDKIVLLSEGKIQAVGTHAE
                     LMEKSVEYRNIVLHQQLESELEAGELDA"
     gene            54771..56552
                     /locus_tag="PLP02_00805"
     CDS             54771..56552
                     /locus_tag="PLP02_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42038.1"
                     /translation="MPRGYGGDIERHRTYSDKTIISRLLVYVKPFIKQFVTALVMALI
                     VVGLDLIGPLLISDALDSLGEETIVFARILLIVGIYFAATAIGAIISYKQTILLQTTG
                     QKVIYDIRADVFQHIESLSIETYNHMPIGKLVTRVTSDIDTLNMLYTDVIINLFRNAI
                     TIIGVFVIMFFLDWKLTLLVLVVVPFIVLASFLFRKYARKAYRNVRNDLANINAFLSE
                     HLTNMKLIQIFNREDRKFSEFKRQNQKLNRSYLQQTFTFGIYRPLMYLLYVVALMIIL
                     WQGSLDTMLQIITFGVLYAFTQYINKFFNPIQDLAEKYDTLQAAFTAGERIFEIMDTS
                     PLVKDDLDAIDIESLKGKIEFRHVWFAYDNEDWILKDVSFIVKPGETLALVGATGSGK
                     TTIINLIVRNYDIQKGEILIDDIDIRKIRLASLRSKIGQMPQDVFLFSGTVESNIRMR
                     DESIEMDKVVQSAKYVNASHFIEKLPGTYLEPVRERGNNFSAGERQLLSFARTVAHDP
                     SVMILDEATANIDTETEALIQESLGKMMNIGTMIVVAHRLSTIQHADCIIVMQKGKIV
                     ESGNHHDLLRKKGYYFGLYQLQYERPE"
     gene            56628..58148
                     /gene="cls"
                     /locus_tag="PLP02_00810"
     CDS             56628..58148
                     /gene="cls"
                     /locus_tag="PLP02_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPF42039.1"
                     /translation="MKRIFLLVALLIVVMFIYTFSLAQYISMSFLLDILPNNIFIYII
                     IGFLWLLAFFIAIRIIMKQTYAYKKSYWILIILLNPILGIILYAIFARDFVADRFHRT
                     RPLIANKAFLGLEEATHVDYDKREYGNIFRFIRENTGRSVYQDDTYVEILNNGDIFFP
                     RLKDALEKARNYILMEFYIIKTDEIGCQILDILKSKAKQGLDVYLIYDHFGSNRHLDK
                     KYMKDLESSGVHIGVFDPQTLSVFNSNLNFRNHRKATVIDGKIGFVGGMNLADEYNHK
                     SPKFGFWRDTHVLMAGNGVTSVQNVFVKDWYYITKEVLDMPMDKTDANYRGLFSVIES
                     GPDFEDGQIRDVYLKMMFAAKKTIDIVTPYLIIEPEIMAAIHVAVKSGVKIRLLVPGL
                     SDYVMVGLATKSYYEQLLRTGVEIYEYKDHFVHSKILIVDDKIASVGSVNFDPRSFHL
                     NFEVTAVFTDEAVGVLSESFREDLEKSNRITMEEWARRGAMKRFIQALLNLFSPIF"
     gene            58174..59484
                     /gene="rmuC"
                     /locus_tag="PLP02_00815"
     CDS             58174..59484
                     /gene="rmuC"
                     /locus_tag="PLP02_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPF42040.1"
                     /translation="MSLPEILGLISVILLTIILVGIAYLVFRRHPKDDGFAGMDRLET
                     DLSKQVGDLKDTFMKTMFESMRTFSSDVNKQLLETSTKTTGNMADFQVKVNQELASFQ
                     EKINTKLTGDFTGLTNSIDQRMNAINAKVEERLSQGFKDTKETFVQIAERVKVIDDAQ
                     KKIEELSGEMIGLQNILTNNQARGSFGEYQLNQLLFSVFGENDKLYQTQYTIKEQKGK
                     KEAVRADAVIFLPKPNNLVAIDSKFPFSSYSKLFDNDELTKEDEDKLIQSFGHEVRKH
                     ITDIADKYIVPGVTAEYALMFVASDGILALLHSRLTNVIDYARSKAVTIVSPTTLIPL
                     LSSYWAMVIDYEQSRYAAEIKKQLGLLSGEFQKFGSDWEKLNRGIDQISRQSRSVNDH
                     VEKITSKFDKIQMVDLPEKPAIQDATDDENDNEAQKDAVPDELK"
     gene            59555..59752
                     /locus_tag="PLP02_00820"
     CDS             59555..59752
                     /locus_tag="PLP02_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPF42041.1"
                     /translation="MNGSVKWFNAEKGYGFITDENGKDIFAHYSAIQGEGYKSLENGQ
                     AVSFDVVEGARGPQAANIVKL"
     gene            59876..61222
                     /gene="cysS"
                     /locus_tag="PLP02_00825"
     CDS             59876..61222
                     /gene="cysS"
                     /locus_tag="PLP02_00825"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPF42042.1"
                     /translation="MQIFNTYTNQLEEFVPLKKGEVSIYVCGPTVYNYIHIGNARPVV
                     VFDVVRRYFEYKGYKVKFVSNFTDVDDKIIAKAKQEGKTEKEISERYISQCLSDVQAL
                     GSETDYIKPKVTEYMPHIIDYIDELVKLGNAYQKDGDVYFRVSSVPDYGKLSNRRIND
                     LISGARVEVNDLKDSPLDFTLWKKTDTGVKWDSPFSTGRPGWHTECVAMIDDIFHGMI
                     DIHGGGTDLIFPHHENEIAQAEAMHHNHIARYWMHNGRLNLEGEKEKMSKSQGSVVLV
                     KDIYDKAMPFRLLLLSTQYRTPLNYSDDNLNMYSAEWDKIVATINSVHRTLAFDNYFS
                     QQNDNVKSNEVSALMQAFEDAMDQDFNTPNAITSLQALVKMANVELRKKNNDPLLADI
                     EKTLMKMLGILGLDPEIRDITEEDKTLYLQWQKLRQDGNYAEADKIRDILQKRKLL"
     gene            61219..61599
                     /locus_tag="PLP02_00830"
     CDS             61219..61599
                     /locus_tag="PLP02_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HPF42043.1"
                     /translation="MNLQNGLSLAYIGDAVYELEIRKRLLDKGLTKVGDLHKQAIRFT
                     SAGGQSKAMDLLEATLSDEELSVFRRGRNTEGGRKPKNSDLGTYHRATGFEALIGYLY
                     LEKKEDRLTELINACFAGVSENPE"
     gene            61684..62400
                     /gene="rlmB"
                     /locus_tag="PLP02_00835"
     CDS             61684..62400
                     /gene="rlmB"
                     /locus_tag="PLP02_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPF42044.1"
                     /translation="MSIYIYGKNPVQEILNSGKQIIQAFVMEKTNGDLLTQFQNRDIP
                     TTFMDRKNFDSKFPGVNQGVAVEIKNYETVTIEEFLKKIDPKDNPLVLMLDGIQDPHN
                     LGAIARSAEAGGVKGIIIPQDRSVGINATVVKASAGAIEYMDIITVTNLVNTAEKLKE
                     AGFWIIGTAADATVSYTEAYVDRPVCLVIGSEGKGMHRLLREHSDLLVAIPMVGKINS
                     LNASVGAAILIFDILRRKKG"
     gene            62404..>62601
                     /locus_tag="PLP02_00840"
     CDS             62404..>62601
                     /locus_tag="PLP02_00840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HPF42045.1"
                     /translation="MLFRNYNDFEIISLIKQGNEEALELMIDKYRFLIAKKIGKFNLA
                     DEYDDAFQEGVIVLYRSVMRFE"
     assembly_gap    62602..62701
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            62804..62950
                     /gene="rpmG"
                     /locus_tag="PLP02_00845"
     CDS             62804..62950
                     /gene="rpmG"
                     /locus_tag="PLP02_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPF42046.1"
                     /translation="MKDKAVLVCQECHSRNYEIKKSDNRTTRLEVTKYCNKCKKHTLH
                     KESK"
     gene            62965..63489
                     /gene="secE"
                     /locus_tag="PLP02_00850"
     CDS             62965..63489
                     /gene="secE"
                     /locus_tag="PLP02_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPF42047.1"
                     /translation="MAEDVTKKPVAGEEKANKKPLKVKKSKMEMEEIHSRVMDILKKE
                     YKFENWLLGILSPILILYGVYIVMGTFGSFDLAGALGDSEIPFFAFFFDTTLKRVLTG
                     SFLILIGTLVIIYLVIPFLRPSFVEMKKVSWPTIKTLARNTARVFLFLIFLSVVFALF
                     GFALDPLFKWLISL"
     gene            63510..64052
                     /gene="nusG"
                     /locus_tag="PLP02_00855"
     CDS             63510..64052
                     /gene="nusG"
                     /locus_tag="PLP02_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPF42048.1"
                     /translation="MDSSKRMWYIVQTYSGFENSVRDNLQRRIETMQMEDLIFQVMIP
                     EELRTEKKADGSIKEKIVKMFPGYVFIEMIVTDDSWFVVRNTPGVTGFLGSSGGGTKP
                     VPLLPDEINPILKKCGLLQAQKLNAQPGQNVKVATGPFAGQIGVIDSIDDEKGKVNVL
                     VDMFGRKTPILLDFADIEII"
     gene            64177..66477
                     /locus_tag="PLP02_00860"
     CDS             64177..66477
                     /locus_tag="PLP02_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42049.1"
                     /translation="MLKLVNVSKYYNSNNVVALGLRKINLELGVNEFVAVVGESGSGK
                     TTLLNVISGMDSYEDGEMYINGQETSYFSINDLENYRKKYIAFVFQNFNLIDSYTVLQ
                     NVELPLLLSGKTRKEARAMAMEIIKKVGLEKHARHKATRLSGGQKQRAVIARALAKDC
                     PIIAADEPTGNLDSESAAQIMKLLHEIAKDKLVIVVTHDFEQVKEYATRKIRIYDGEI
                     VEDVQVHHSKPADLPEIEENLKRLKPWEQVKMAFVNLMAVPKKTILMVFVFFFLSSFV
                     ALVYGAYRISVSYVDQYYYYTSDDEFFSNTSISRVILNKKDYSPFTASELADLSDIPN
                     VQTVIYNDYLIDVSIYMISNEQVANNGRSRNMLADVLILPMSVIKDANVLSYGELPDE
                     EGEVLLALPAAELEKSDLYLNNAYTMNGFSNIYLYDTAEYLITGLVDAGDLFMDETND
                     YIIVSDAEFARLVKVCYLAFTKTLSLDVLDSEGDDLGLGEWFSSMFMYNGLQILVDDS
                     LTDSEIGFDTALSDYLCYDPYGTGDHCGIEFADLTVEDAYHDLSLSDLEVTFIDNQWI
                     AIYLSQNNYDRIFYSDIYQVSLLTKSDINVGRLVSDLVKIKDGTEAKYNVVYPYEFET
                     DDQFGSAMSFFQLLGMLMTVLATALASTLLSYIIFRAVINTKLKDYTIFRTVGATQGV
                     IRLMIYLEDLFVALFSFLLFVIMTVSFKLSDAITRYSAFYGLKAFRFQDYVLFFVILI
                     LMSIFISGRYCNRIFKDTVTTTMKRG"
     gene            66484..>67231
                     /locus_tag="PLP02_00865"
     CDS             66484..>67231
                     /locus_tag="PLP02_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42050.1"
                     /translation="MIKIIDLNKYFNKGRSNEIHVIDHTSLEFPETGLIAITGPSGCG
                     KTTLLNVIGGLDRFHSGEIDFDETKIMKYKSLQWDLVRNKYIGYIFQNYNLLPDKTVF
                     ENVEISLNMAGLYDKEKVEERINYVLESVGMYNYRRRNVQALSGGQQQRVAIARAIAK
                     DPKVVLADEPTGNLDVNNTFEIMSIIKKISQTCLVILVSHEREIVDFYADRVIEIKDG
                     KVVNDYENAGNKTLEHVDSRNVYLLDLEKEE"
BASE COUNT        19828 a        14845 c        15838 g        16620 t
ORIGIN      
        1 ccatctggtt cggtagctca gttggtagag caatggactg aaaatccatg tgtcggcggt
       61 tcaattccgc cctgaaccac catcaagccc tgaaacaagc ccttttaggg ctttttttat
      121 taagcaaata aagacatttt tataaataaa taagaaaaac cattttacta tatgttaata
      181 tgttcttgaa agcataacag aggtgtgaaa tggaaaaaaa aatcacattg agacaccatg
      241 tccatgacta tgaatgcatg tggaacggca ttgaagatct gtatattgcc aaaagtggcg
      301 aagcattgcc ggaaagcttc ttttttttgt tagcgtcttt tggcgcattt tgttatcttc
      361 atacaaacag tcaagatata aaacgaatga tcatgttcgg tgatggcagg acaaaaaaaa
      421 tgtatgaata tttggcaccg atcgtagaat tccagtacaa acactactcg tacacatcgt
      481 ttgattcctt gctgaagacc gtgatccggg agattgacga cgacaaaccg gttgtcctag
      541 gggcgatgga tatgttttat ctcccttatt tcgagaaaat atatcataaa gagcatattc
      601 cctttcatta tgttctagca aacggatacg atttggataa aaagacggtt tatgtatatg
      661 acggcgccag agaaaatcaa atagggctat ctttcaagac gcttgaagaa gctttagatt
      721 gctattctcc cggtctaagc aagaaaaaca ctttatgcaa aattagaatg ggagaaacgc
      781 gttccaagta cagcatttca aaacttgctt tggcaaaaaa agctcgcgct tttctgaacc
      841 cgccgaccaa ttttctcgga atcaaaggca tggaaaaact gattgatgaa atcaaatgct
      901 gggataaaga actaagccaa gaagatatcg atcaaatatt ggagaatatg ctgatgtttc
      961 tgggaagcgt tcccacatta ccaaacagac tcaaaaagct gaacgaaaaa gaccaggtca
     1021 cttttaaggg taattacgat aaagtatgtc agatgatgga cgtgctggca aacgaatatg
     1081 ccgacaaagc atatctaagc acaatacctt acttttatgc ttgcgcacaa actatggaaa
     1141 aaatatcaga tttgatagtc gaccatttgc ttatgcatgc aactacctca gtttcgatgc
     1201 cgttattgtt ggcagagatc aaagataatt tggaaaaagc atttaaaata ttattgtaat
     1261 attttccgat aaatacatag cttcatctga caagaatgct cgaaagccgg ggttcaaact
     1321 attaaacggg gacatatact ataattattt tggtgatatt catgaacaag taataatttt
     1381 aagagttgca taaaacgact tatttagaga ttttttgtca caagaaaaag aatcataaaa
     1441 ctgctttacg aaaagaggcg aaaaaattgg atatcttaat tcaacaatat ccgaatgatt
     1501 cttcattcgg agaaacattc tgcgaagtcc gtgatttttt attccggatc aacgaaaaag
     1561 acctttcgac ggttgacttc ccctggggaa gatgggaatg ggctttttcg ttgccgttcc
     1621 ttcctgtaga caggttaacc aaaatggcta tttggcggca tgagaaaaag atcgtcgcca
     1681 ttgtatctta tgaaagcacc gcctgggatg cctattttgc cattgatccc gaatattctt
     1741 ttttgaagga agaagtgctg aaacacatag caaacgaaat gccaggacca gacgggatcc
     1801 atattttgat cccagacaag gacattaaag cacaggcgat tgccagggaa atcggatttg
     1861 tttccggcac gggcaaagaa tacaacgccg tgatcgatat cgacagttcc ataaactatg
     1921 ttttgcctcc cggtttctcg ataacgagtc tcaatgaacg atttgatctt tgtgaataca
     1981 accgcgtgct ttaccgggga ttcaaccatc ccggtccggt ttcagacgat cacgaagaaa
     2041 tcatgagcag gctgaaaagc ctatcgggtc cggacgtgga tttgacactt aatactgctg
     2101 tagtggcgcc aaacggtcat ttcgtgtcat attgcggcat atggcacaaa aaaggatcca
     2161 aatacgcatt ggtggaaccg gtcgccaccg atccggaata cagacgtatg ggtttgggga
     2221 aagcggcggt gcttgccgca gtcgcaaaat gcggcttaca gggagcttta aaggcgtttg
     2281 tcggttcgcg tcaggaattc tattatcgcc ttgggttcaa gccttattcc gaagcgacgt
     2341 tctgggagcg tcccaataag taaagccgat aggaaagagg attccgatga tagtaaacca
     2401 aatcagaatc aaaccgggaa aagaaaccga atatccgttc aatctgacga tttttaaaca
     2461 agaaatcatt ctggatgtcg aaagaccggt tacggttttt gtgggagaaa acggaagcgg
     2521 gaagtcaagc cttctgaaat tgatccaatc cagaatgcgt ctggtggaaa tcaaatggca
     2581 ggacaatccg gaagaaaagc acgctatcga tgcttcgaca gtagatatta tgccgaaact
     2641 cggaaaaccg agaggtttct tttttgaaag ccttaaattc atcaattata tggaatatat
     2701 gcaaaaagaa atcgcatatt caaacgcaga actcgaacgc gtggacaagg aatacaaaaa
     2761 cagatcccct tatgccaaaa tgatggcgga atctccgttc aaacgaacga tcggcgaact
     2821 tgaccgcatg tattccagag atttgcctca ctcgtcgcac ggagaggcct atcttgattt
     2881 cttcgcgtcc aggatcagag acaatcaaat ctatctgttg gacgagccgg aaaccccgct
     2941 gtcttttcaa aatcaactga cgcttgccgc acttatcatt caagccacca aaaaaggttg
     3001 ccagtttatc atagcgacac attcccctat catcgccgcc attccggaag ccttcatata
     3061 tgaaatagaa gaaaacaaat ttgtccggac gaatttcgat gaaatcaagg atatccagat
     3121 gcttaggcaa tttctgaatc acaaggaaca gtttttaaga catttggacg aagatgattg
     3181 aaacaacgct ttcttaagaa aaaacagaaa ggtattgatt ttcttcttta aatgatttaa
     3241 aaataaaacg atacaaaaca atttttatac tcaggaggcg caaaatgtat tgtaagaatt
     3301 gtggagcaga aataaaagaa gaaaccggcg tttgccagaa ttgcgggatg gcaatgttac
     3361 cggaacccgt tatggaatct aaagccactc gaaaatcctc aataagactt tcaaaattgt
     3421 tctccatgct tcttggaatt gctatggtct ccggactggt atgcttatgg ctgtctgtgg
     3481 cattaggagt cgtttattac gtaggcaatc actatgaaaa caattattat ttctatccgg
     3541 attatgtttt ggctgtttca gctttggcaa tatacatatc aacaactatt ttgtcggcgg
     3601 ggaatttggt attaagttgt ctggaacaga aaggaaataa aaacccggaa tcgattatgt
     3661 tggccgtttt tatactggtt gcctcatcgc ttatgtcggt cgcggcatac gtaatgaact
     3721 atttctctcg ttgattgaaa acaaaaagga gataacgaaa catggcaaaa ttctgtgtat
     3781 attgcggagc cgaacttaaa gagggagccg acatttgttt gagttgcggt aaattcgtcg
     3841 ttgacgacaa cgataagtcc gccatcaagg aagaaaaaaa gccagcaacc ggccgacttt
     3901 gttgcgtaag cgggatattg ctgacgctga ctattgtttt ctatatcacg gcaatcctga
     3961 aagcgtgggt ggattccggc aatcattact tttggctggg aaatgaccac tacctggcaa
     4021 tgattctggc tgctgcgttc ttcgtgatgt cgatagttac ttttgtgatt gccattacgg
     4081 aatacaaagc caagaagatc gggctaaacg aaagatacat atccatattt ctttttaccg
     4141 tcgggatgat aatgccttat gtgatgatgg tcgattggtt tttccaacaa tagcgttatt
     4201 ttataatatt tacaaaatca tataaaaaca tgcggcatca acaaccgcat gttttttgta
     4261 tacctgatca tttttcctca accgcagcga atactttggc gatatcgtcc tggatcagaa
     4321 ggtaatcgta tgaatcgcgg aaatgtccgg attgttctcc tagattcacg acaaccagat
     4381 gctttccatg aaaatacctg atcagtccgg cggccggata aaccatcagc gatgtgccac
     4441 cgacgatcaa tgtatcggcc atagcgatcg ccttgacgga cttttcgata acatcctcgt
     4501 cgagagcttc gccatacaag acgacgtccg gcttgattac accgccgcac gggcatttgg
     4561 gaatgccggt tttgtcaagt tcttccaaag cgaagaagcg atggcaggac tggcaataat
     4621 tgcgatgcac tgagccatgg agttccatga cattcttgct gccggccatt tgatgcaacc
     4681 cgtcgatatt ttgagtgacg attgctttca gttttccttc cttttcccat tttgccaaga
     4741 cctgatggac tttgtttggc aaggcgtcgg aatataccag tttggaaaaa tagaaattga
     4801 aaaacagatc cggatgcctc gtgaaaaaat cgtgcgatac catttcttcc ggagagtatg
     4861 tgtcgttcaa acgttcggaa tatattccct tcgcggaacg aaaatcggga attccggacg
     4921 ggacagagac gccggcgcct ccgaaaaata cgattgaatt cgattctttt actatattac
     4981 gaaatttatc tatcttgtca tctgtcaatt caaatcagct tctttcttct tatttattgt
     5041 atcactaatc gaagttttaa gagctttcga ttcttgatgt aagcaaactg actgaaaaac
     5101 attggctttt gtaatatatt ttggtaaaat atgaaacata ggggagtagt cagcccgtaa
     5161 gggcggttgg cctacacact ggcgcaagcc aggctcaacc tgaaatgacg agacttatgc
     5221 tttttggcat gagtctttta ttttttgtga ggtacatgat gggaatattg gaagtagtgt
     5281 tgcttgcggt cggtttagcg atggacgcca ctgccgtgtc attggcgaat ggcctatgcg
     5341 aaacagatct gaaaatcagg aaatcattgc tgatggcatt tttgtttggt gcttttcagg
     5401 cgtttatgcc gcttgtcgga tattttgccg cgtctttctt tgctggattc ttatcgagga
     5461 ttattccttg gcttgcattg ataatattgt ctatattagg aataaaaatg atcacggaag
     5521 gaaaaaaaca tatttcctgt gattgccggg aaaggcatat atcgttaaag cgcatgctta
     5581 ttcaggctgt cgccaccagt atcgatgcac tggctgtcgg cttgcttttg gtggaaacgc
     5641 agctgtcgcc gatgttggaa tacgtagcca ttatcggcgg gatcactttc cttttgtcga
     5701 tgattgccct acgtctcggg aaaagaattg cgcccttttt ggaaaataaa gccgaatatg
     5761 ttggcggagt tatcttgatt ggtatcgggc ttaagatttt catcgagtat ctaatatcca
     5821 tattttagca atgccggccg catgataatg cggctttttt tattcaagta atcagaaccg
     5881 tcattcgcgt atcatgttac cgaaaggacg gtataacatg aaaaagaaaa tgcttgtgac
     5941 tgccatattg ctggcaggcc tgtttttgcc aatgtgtcgg gttttggcat acaatccgaa
     6001 ctgtcttcca ggaggagata attatttgtc ttctgacaat tttgttttga atggggatca
     6061 atacgagaca attgcgccat ttcttgtcaa accttacact gattatcttt tttcttttcc
     6121 ggagtattat gaatcagtcg aaccatacat caatatcggg ttgtatacta acagcgatta
     6181 catggacgaa ttgtgcttca cgtatgaaaa tttttcctat aaccatgatt atcgagtttt
     6241 cttttattct ttcaggaccg gtccggatat caactacatt tccgtaacct tgtatgacgg
     6301 cggatacata accacaaacg gattcaatgg attcatattg gaagaaggca cgacatatga
     6361 tggttatgaa cgctatattg agggagcaac cgtagatact gatgcgccgt attttcaagg
     6421 aacgaatacg atagtgtctt atgttgacac gccgattacc gtagcgcaaa tccagtcggc
     6481 cctgacggca tacgatgcca tagacggaga tcttacggct tcgatcgtca tggaagcgga
     6541 cggttatacg cccaataacc agatattggg aacgtacata gtgtcatttt cggcaaccga
     6601 ttccagcgga aacaaaaata ctgtagacat aatcgtggaa gtagtggatg tggtttctcc
     6661 ggtttttggc gatataggga caatcatcgc cgtatatccg aacgtctatt ctcccgaaga
     6721 tattaaggcg atgctaaatg caagcgacaa ctatgacgga gatatcagtt cttacatttt
     6781 actcacaagc gatggatata gttccaataa tcaaaccgtc ggatcatacc agatggaatt
     6841 ttccgtcagc gattccagcg gaaatcttgc gacccatacc gtgacagtcg aggttgtcga
     6901 ccaagaagcg ccggtgatat ccggcatatc ggaaatcaat atcggttatg atgccgtgat
     6961 aaattcgagc tatgtcaaat ccgaattgtc ggtcatcgac aactatgacc ctgtcggatc
     7021 catgtcgctt gtgcttgtgt cggatactta tagcgccaat agccaaacaa taggaaatta
     7081 cgcaatggtt ttcgaggtgt acgatcaaag cggtaacaga agcgaaaaaa cggttttgat
     7141 aaatgtcgtc gacagcatcg tcccgattgt ttatttcgac acatccgtga tccaagtcta
     7201 ttccgacagt gtgcttacat taatcgatat caccaagatt ctcggagaag ccaaagaact
     7261 ggacatggcg ttcgattacg atgtgttcgt tttatatgat tcatactcgc gtcacgcatc
     7321 cgtcccggga acatatcaca tatctttgag attcgtgccg tcttcaggcg gtgtcatgga
     7381 caagaattta caaatatgcg ttatagacag aacgccagat ttccatgttt cagcaattcc
     7441 ggaagaagga caaaccgaag ggtttttcag ccgaaatatt tggtatatcc taactacggg
     7501 agttatgatc gcaagcggag gaattgtttt gacgataaaa ctcgttttga agaaaaaaca
     7561 ggtcgtgtga gatgcctttg gctttaattc ccaaagccgg cgtgttataa tatgtctgct
     7621 cgtgcgaaat gcactaaata tgccggttca ccaaggggga atggaagatg aaaaaaatat
     7681 tgtcggcatt gcttgtcttt gtcatggttg gcgtcttatc ggcgtgtacc cagaaagaat
     7741 tggttacggt tatggtgccg tccggatcac cggcgctggc gcagctttat atccaggaag
     7801 atactgagac ctatcaagtc gatgtcgtac agggagcgga tccgttaatt gccgcattca
     7861 cagcgggaac gcatgatttc attttcgctc cgacgaatct tggagcaaaa ctttataccg
     7921 cttctcccga ctatgtttac atagcggcag tcgtattcgg gaactattat ctggttgcgg
     7981 atatgggaga tagcgaagat actttcgacc tgtcatatct tgacggccgg gaaatgactg
     8041 catttggcgc taattcgaca ggcgacatcg tgatgaaata tatttttgat tcagccgata
     8101 ttacgcctaa catcatctat gttgattcgg tggccacggc gacatctctc cttatatccg
     8161 gggaaacgga tatcatattg acagctgaac cgtcattatc ggtattgctt gcccaaaacc
     8221 cgaactacgc ggttatcgac ttacagacgg agtatgagaa cctgaccggc gaatcgtctt
     8281 atccgcaagc cggcgtattt gcgaaagcca cgcttaccaa cagagtcata aacaattttt
     8341 tgaaagatct tgaagattcc atcgaccagg taaacgaatc cccgtctgcc gccgccactt
     8401 tggcaacaac actcgggtat acgttcggag aatcagtatt gcagacagcg attcccaaca
     8461 gccatttggg atttgtcgga gccttggatt cacgaagcga tttggaagct tatttcacag
     8521 taataatgtc ggtcagcgct cccttgatcg gtggcgccct acccgttgac gatttttatt
     8581 atcaaccttg acaatagggt gatgtgattg aagaagacct atcttttcct attggccgtc
     8641 ttcacgatat tctgtatctg gatgatcgga tacgtcgtgg tttcgagccc tttgctcatg
     8701 cccgatccat atgcggtgct taaggccctg gtcgggtttt ttggcaaatg gacagctgtg
     8761 aagagcatcc tcatgacgat catgaggctc gttatcgcga tggccgcggc tttgcttctg
     8821 ggcggaagcc ttgggataat tgccggattc catccgaaat tttccgttta tatgcaaccg
     8881 ctcgtggcta tcctgagaac gataccggtt atctcggtaa tcgtgattat tttgatcgtt
     8941 tttggatttg agacagctcc gtatgtcatc acctttctga tgattttccc tttgatctac
     9001 caggcgtttc acgacggtat cgaacagctg gaccaggaat tggtcgacgt ctacagactt
     9061 gaagacaata actggttcac cggtcttcgt ttttgttttc ttccgcttat aaaaaaccag
     9121 atcacaaccg ttcttctgca atcggccgga ctgggaatca aggtactagt catgtctgag
     9181 tatctttccc agaccaaaaa ctcgatcggg aattctttat atcttgccag aattaatttg
     9241 gcctacgacg aggttttcgc ttggacggtc attcttataa ttatgacgtt gtcgctggaa
     9301 cttgtcatca atcattttaa gaatcgcgaa caaaaagtaa cggtttccag tacaggcgtc
     9361 aaatcagaat tggattgact ttaagccctt gttaatatat tatatttatt gaacataggg
     9421 gagtagctgg cgggcacccg cggttaaatc aacaagcatg gcctaggcct ggtttaatct
     9481 tcgacagcga gacttatgca cggtttgttg cataagtctt attttttagg aggcaaacag
     9541 atggatgctt atcaaaagaa tacggaagaa ttgttgaaag aacaaaatgt gagactcaag
     9601 gaaggattgt cttctgaaga agccaatgcc agaatcgagc gttttggccg taacgaactc
     9661 aaagaaaaga aaaaaaccac actttttcag atgtttcttg aacagttcaa ggatttcctg
     9721 gtaatcattt tattgggtgc tgccgtcgta tcgatcatta ctgcgatttt cagcggtgaa
     9781 ggattggtcg atggaatagc gattcttgct atcgtgatta tcaatgccat tttaggcgtg
     9841 tctcaggaac aaaaagccaa taacgctttg gctgcgctaa aaaaaatgag ttcgccgcac
     9901 gccaaggttc tcagagatgg aatagtcaga gaaatctctt ccgtcgattt ggttcccggc
     9961 gatatcgtat atttggaaac cggcgactat gtggccgccg atgtcaggtt gctcgaaagc
    10021 acgaatctgc aaataaacga atcgtccctt accggcgaat cgcaacctgt ggaaaaagac
    10081 gctcgcgtag tttatcacga tgatacagtg ctggctgaaa ggcataatgt cgcttatatg
    10141 tcgaccttgg tgacttacgg taaggctatc ggcatcgtga caggcaccgg catggaaacg
    10201 gagatcggaa aaatcgccac tatgctggaa gctgtggaag aagggaaaac cccgcttcaa
    10261 aaaacgatcg atgtgttcgc caaaatgctt ggattcatct gcatcggcgt ctccgtagtt
    10321 gttttcgtcc tcggtttgct cgaaggacag aaagtattgt atatcctgct taccgccatc
    10381 gctttggcag ttgcggctat cccggagggg ctgacagccg tcatcaccgt tgttcttgca
    10441 ttgggaatgc aacggatggt taaacggcac gcaatcatca aaaacctgtc gacggttgaa
    10501 actttgggac aagcgacggt catttgctcc gacaagaccg gaactctcac ccagaacgaa
    10561 atgaccgtaa gaaaagtcta tgatttagaa aaagagtacg atgttaccgg aaccgggtat
    10621 gaactttcgg gcgatattat ccataaagat aagaaagccg aagtaaccgg aaaccttgat
    10681 accataatga aaatcagtct tctttgcaat gatgctatca tggaagaaaa gaaagttctt
    10741 ggtgatccga cggaaggggc tttacttgtt ctagccgtca agggtggtta caccattgcc
    10801 ggaaaagaag acaaataccc gcagataaac gaatattcct tcgattccga acgcaagatg
    10861 atgtcatcca tcaacaagat taatggggaa tactacgttt tgaccaaagg cgcctgcgac
    10921 cagctgatca aaaaatgcga ccgcgtcgag atcaatgggg aaatcagacc gatcaccaaa
    10981 aaagacgtgg agaaggttct tgctaagaac caggaatttt ccgaacaagc ttatcgagta
    11041 ttgggatatg cttatcaaaa gaccgataca cccgaagcga atgatctcga acagaatctg
    11101 atattcgttg ggttgactgc tatgattgat ccgccgcggc cggaagcgaa aaccgccata
    11161 gaggaatgtc atcacgccgg tatcagggtt atgatgatca ctggcgacca tttggcaacc
    11221 gccaaagcca tagctagtga attgggaatt ttaaaaccgg gacacctggc tttgtctggt
    11281 gaagataccg ccaaaatgac cgatgaggaa tttgaggaag cgatcttgaa atgcgacgta
    11341 ttcgcaagat cgacaccaca ggacaagata aggatagtcg agattttgca aaaacatgga
    11401 gaaaccgtag ccatgaccgg cgacggcgtc aatgattctc ccgccctgaa acaagccgat
    11461 atcggcgttg ccatgggtat taccggaacc gaagtttcca aggaagccag caatatgatc
    11521 ttgaccgacg acaacttcgc ttcgatcgtc aatgcggtcg aagaaggccg gatcatttat
    11581 gccaacatcc gcaaattcac gatctacttg atttcctgca acatcggcga gatcctggtc
    11641 atcctgttag cgatggtatt cagtttcctg ttcaaggcta tagatccgga aaccggcgca
    11701 ctcgtttccg tgttaccttt gtcggccatc cacctgttgt ggatcaacct gatgaccgac
    11761 tccttcccgg cattcgctct cggaatggaa aaaggcgaaa agggcatcat ggacgagaaa
    11821 ccgcgcagtt ccaaggaaaa gcttctgaac aaagaaacac tgataaaagt ctttgtacaa
    11881 ggatttggtt tatcggtggc agccctgctc agtttctggt tggggcttaa tgctttcccg
    11941 ggagccgatc ttttccaggc gaggacgatg atattcgtaa ccgtcgtgtt cggagaactg
    12001 ttccgggcct attcggccag atccgaaacc aaattcatct gtcagagcaa tccatttgcc
    12061 aacaaatatg tcaactacag cgtttttgtt tcggtgggct tgctgctact attggtatat
    12121 atcaaaccgt ttgccgaggt cttcggcatc gaagcgctga gcggtatgga actattcgtg
    12181 gctttgtcac tcggattcat tccgatgtta tccggagaag ttaccaaatt ctttcacggt
    12241 aaaaaaaacg ataattcctg atttttgcct tcattattga atttatccgg cgtatgcgtt
    12301 aaaattattt taaagaaagc gcaaggatgt gatgaccctt gagaaacaac gtatccaaaa
    12361 atgttactct ggcccccgga gagaatgtcg ccaaggattt tcccaaactg aaggcgagaa
    12421 gaagcttttg ccaaatcatg ctgacaacga aaagattgat tatttattcc aacggacaat
    12481 caatgtccaa cggacgtaaa gtcaatcgca aatcgatgaa tgaaattgat ttgaattcca
    12541 tccacaacta cgagtattat gtcgaccggc accggcactc gcttttcgta aggattctcg
    12601 gattctttct gatcgtcgcc gccctagcgt ttgcctatat ggcgatcatc ggcatggtaa
    12661 ccatccaaag ctatccgtat tcgtatattc tcaattacgt tgttttggga ctggtcgtca
    12721 tgctaggctt gttgatgatg tttgcaggtc cgaaaacctt gaccgttatc atcacctccg
    12781 gaatcaataa tgtcgtgaag ttggcgcttg aagccaatca ttacaacgaa ctggccgtca
    12841 gatatatcgg cagcaagatc catccatcat actgaacaaa cataatatga acaaaaagtg
    12901 gaatctgatt ccactttttt attgcttttt gccatttgcg gggccattta tggtataata
    12961 gtcccacata tgaaaaataa taaaaacaga ggtgaacaat gaaactcaat tataaaaaga
    13021 tatttgcggt cgggttggct tttttcctaa tttcgatgtt ttggcaagcc tatgataaca
    13081 tcatcgccaa aatcctgatc gacaaattcg gtttgaacca gacatggtcc ggagctgtca
    13141 tggctttaga caacgtcctg gctttgttcc tgttgccttt attcggagcc ttgtccgaca
    13201 ggaaaaactc caaactgggg cgccgtacac ccttcattat cgtcggtact gtcatcgcgg
    13261 cgtttgcttt catgggaatg tcgtttgtgg ataattacca gaccacgaaa atccagacgg
    13321 aaacaacgat tatcgaagat tacagttcct tgaaaacaac cgaacttacg gaagtatcgg
    13381 attgggaaac tgccctttcc gacatgcttg cggaaaggga cgccgcactc gccgaggaaa
    13441 acataaccca gagccagtat gacaaatgga aaaccggcac ctatgacaag atgcaagcta
    13501 ttcttgatga tgccaccggc actttggacg aagattccat cgatagtctg aacagtttgt
    13561 attacactta tttcagtact cagatttggc attggaacga tgtcattact tccatgtacc
    13621 aggaacgttt cgccgcctta caggacgacg gaatcacccg ggaacaattc gattcatgga
    13681 aaaccaagat tttagacccg atgcttcaga tcctgatcga caatcccgat ggtctcagca
    13741 gtttcgatgt caatgacatc aacgacctgt attacaatta tctaagtacc agagcctggc
    13801 agttaaccgc atcgaatccg acgaacttcg ttgttttcat gcttgtgctt ttcgtagcac
    13861 tcgtagccat gtccacattc agatcaccgg ctgtagcctt gatgccggat gtcacaatca
    13921 aaccgcttag aagcaaggcg aacgccataa tcaatctgat gggaactctt ggtggcttgt
    13981 tgattctcat cttgctcaag atatacgcca tggacaatct atctttcgtg cattacactc
    14041 tcgcctttgt atcagttggt atcctgatgc ttatttttct gggaatattc ctatggaaag
    14101 tcagggagcc caaactggtc agggaaaaag aggaagaaga acgccgttat ggtctgactg
    14161 aggaagagga agccaaggaa gtcggcgaaa ctgtcgaaga attgtcggca gagaaaaaga
    14221 aatcgcttta tctcattctt ttctccgtat ttctatggtt tattggttat aatgccgtta
    14281 cgagcaaatt ctccgattat gcgcctaaag ttcttgaact tggttattca ttaccgctca
    14341 tgattgcata cgccacggcg ttgatatcat ttattccgat cgggattatt gctacgaaga
    14401 tcgggcgaag aaaaaccatt atgatcggta tagttattct gactttgtgc tttggatcgg
    14461 cggctttcct tacaaaggat tcctcgtgga tcatgtatat cattttcgga ctcaccggca
    14521 taggttgggc gacaatcaat gtcaactcct atccgatggt tgtggaactg tctaaaaatt
    14581 ccaatgtcgg aaaatatact ggatattatt acaccttctc gatggccgct cagatcatca
    14641 ccccgatttt ctcgggattc ctgatggaca aaatcggccg gattgcctta ttcccatacg
    14701 gagctttatt tgtcgcatct gccttcatca cgatgatgtt cgtcaaacat ggagacgcca
    14761 aggcggtcaa gaaagattcc gtgatcgaaa actttgatgt agatatggac tgattttttg
    14821 attgaaatca gaaaaaagga cgccatttgt ttaggcgtcc ttttatatgg ttttatggtt
    14881 cgatgaattc gaatacggcg ttgtcgtaat attttttgac aaacgcaaat tcctccatgg
    14941 ttctggcttt atatccaatg acagtcagtc cccgtttttt ggcccgattt aaaaaacgat
    15001 tgggcatgtc tcgtaggttg tagttgatga agtctggccg ggtgaaagca ttgaggagca
    15061 tgcgtttcat gatgaatttg gtgatttttt tcatgtcggt ttcttctagg aaatattccg
    15121 ctatcacacc gcgcaagatt tccggatggt tttttctgaa ccacagtaac acctgcggat
    15181 ggaaagaatg cattgcccac acacctttgt agtccttcag gatttccatg gctttggcac
    15241 aaagcaattc gatacttccg tgatatttga attcgataag aagcgggact ctcccatcga
    15301 ccattttcag aaattccgtc aatgtgggaa ttgtttcgtc ggtatcgttc agtctcagtt
    15361 tccgggcttc ttccaaagtg acatcgaaca atttgcggtc atcaccgcac attcttttaa
    15421 aggttttgtc gtgaaaaaca accacagttc cgtctttcaa aacattcaga tccatttcca
    15481 agccatatcc tttttcgatt gccaggcgga atgccgccat ggaattttcc ggtattttac
    15541 cgtcctcgct atgcaaaccc cgatgggcga acaacccggt tttaatccat tccgtgtctc
    15601 tcatcatttg cctcctcata agcagactac ctatcattat atcatcatac cggccaaaag
    15661 gcgagcatct ttggccccga acaaagcccg atgtggtata atttgtttga aagaaagagg
    15721 tgtttgtttg atggaaaatt tccgttttta cagtcctacc gagatcgtct tcggaaaaca
    15781 atcggaaaac gaagtcggcg aattactaaa gcaacagggc gctaccaatg tgttcatcca
    15841 ctacggcggc gggtcagttg taagaagtgg tttgctggat cgggttatag ccacgcttga
    15901 cgagaataag atccaacatt tcgaactagg cggcgccaga ccaaatccga gaagcggact
    15961 ggtttatgaa ggaatcaagt tatgcaaaca aaacaaggcc gattttgttt tggcggtagg
    16021 tagcggatcc gcaatcgact cggccaaggc catcgccgct ggcagtaagt atgacggtga
    16081 tttctgggat ttttacgaag gtaaggcaag gataaaagat gcattgccgg taggggtcat
    16141 tctgacactc ccggctgctg gatcggaagc ctcccagagc gctgtcataa cccaggaaaa
    16201 aggaatgctg aaaaaaggca tcaccagccg tttctatcgt cctcgattcg ccattgtcga
    16261 tccggaactg acttataccg caccgatgta ccagatggcg tgcggagtgg tcgacatgat
    16321 ggggcacatc atggaacggt atttcacgaa ttcttccggt accgatttaa ccgatgccat
    16381 gtcggaagcc gttttgcgac agatcatctt ggccgccgat aacatgataa aagacaacaa
    16441 agactatgaa gcaagagcta cactttgctt tgccggaacc attgcccata acggaattct
    16501 cggtgtcggc aaaaaagaag actggacaac gcattttctc gagcatgaac tcagtgccct
    16561 ctatgatgtg gcgcacggag ccggtcttgc ggtaatgtat ccggcttaca tgaaatacgt
    16621 gatggatacg aacatcgagc gtttccgcaa actggccatc aacgttttcg aaatcaaaga
    16681 aaccaatgat tcgaagtcgg ttgctttaca aggaatagag gcgctcaagg cattttatcg
    16741 caaaatcggc atgccaacta attttcagga acttggcgta aaaaaggaag acatcccgtt
    16801 gcttttggcc acactcgaga aaaaccgcgg acaaaagttt ggcaatttcc aggaacttac
    16861 tttggatgat gccaaaaaca tttacctgtt agcgtgtgag agctgacaaa agtatctatt
    16921 tatccacaat tgcgacttta aagacatgac aagcgttttt gcatactcgc ccatgaaaat
    16981 aaaaaggcat gggttgccgt atgaaaacgc ttgttttttt tgtgcaatta tgttaatatt
    17041 ctatggtgcg aaaagacttt tttttgtggc gaaaatcgga tggtatttct attcgccgca
    17101 actaaatttg aaaagacaaa acagggaggc tttttttatg attgttggta ttccgaaaga
    17161 aataatgcat ggagagaatc gcgtttcatc aactccggaa accgtccgca aaatggttgc
    17221 cgacgggcac aaggtattgg ttcagaattc tgccggtaat ggagctttct tccacgaccc
    17281 tgaatacgag gccgctggcg ccgtcatggt gggggatcct cgcgaaatat atgcgaaatc
    17341 cgacgtgatc ctaaaagtta aagaaccgca gtacaatgcg gcattgggta tgcatgaagt
    17401 ggaaatgatg cacccgggac aagttcttat cacattcatt catccggctt ctccagtgaa
    17461 tcacgaaatg gttcgcaacc ttaccgccgc cggggttatc ggtttgacat tggacggaat
    17521 ccccagaatc agtagggcgc aatcaatgga tgctttgtcc tcgatgagta catgcgccgg
    17581 atacaaaggc ataatcatgg ccgctgactg tatctccaaa ttcatgccga tgatcggatc
    17641 tgctgttgga atgatcaaac cagccaacgt gttggttatc ggaaccgggg tcgccggcct
    17701 aagagccctt gccacagcaa aatcgctcgg tgccgtagtt tactccgccg atattcgccc
    17761 ggaagcaaac gaacaagcca aatccttggg tgccaagata gtcgaaacgg gagtgccgac
    17821 tgatatcgct gttgatcccg atggaaaaca cgcaaatacc ctttctgaaa aatggctaga
    17881 tgccgagaga ggcaatttga aagaagccgt atcaaaagcc gacatagtgt tcctttccgc
    17941 tttgttgatg ggaaaacgtg caccgatcct tttggatgaa gaaatggtaa aatccatgaa
    18001 gcccggttcc tgcattgttg atatctcgat tgatcagggc ggaaactgct cgattacaac
    18061 tccgggagaa agagaaatca aacacggagt cgtcatcgaa ggatgcaaaa acattccggg
    18121 aatgttgcca acaagttcga cttggatgtt tgcaaacaac atttacaatc ttttcaaata
    18181 tttagcaccg aatggcaaat tcaatcttaa tgaagatgat gagatagtca aatcgatttt
    18241 ggtttgtcac gaaggaaaat tggtgcatgc cgggaccatc gaagcgatgg cccaggccaa
    18301 ctgacgaacg atgacgcttc tccaagccat tttacttggc gttttcgcgg cgtctaccgt
    18361 cataggatac tttttgataa ataacgtacc ttcgcttctt catacgccgc ttatgtccgg
    18421 catgaacgct ttgtccggca taaccgtttt gggagcttta gtggcgacat cagtcgcaat
    18481 ttccggacag aatccgatct tatccggctt tttgggtggg cttgccatca tcgcctccat
    18541 gatcaatgtc gttggtggtt ttactgtcac cgacaggatg ttgaaaatgt tttcccgtaa
    18601 ggagaaacac catgaatagg acgcttgcat tgggattgga agctttctcc aatacattat
    18661 attacgtttt ggttggcgtt ctggtgttag ccgtattgtt cgggatctat cttatgagca
    18721 aggttgaaaa agcgaaaacc ggaaatcgta tcagcgctgt agcaatggcg ttggccatca
    18781 ttttgacgct tgtcaaaaat gccatcttgc cagtttggat attgtatgtg tgtctggctg
    18841 tcggattgat tatcggactt attctggcat ttagagttaa aatgatcgaa atgccacaga
    18901 tgattgcttt gtttaatggt tttggcggtt tggcatcggc aattgtcgga ggatacgctt
    18961 tatttggcat cggctccgga gccgattcat tcagcaggat agttgcgacc atcgcatttg
    19021 aaattggtat cataacattt ttcggcagtt tggtcgccgc aggcaaatta catcgtgttt
    19081 tgccgcaaaa gcaaataaca ctgccaatgc acaaaatatg gcttattgct ttgtttgctg
    19141 gatgcgtgtt tgggattatg ctatcgtttt tctcatctgt cgatccaatt atagtagcga
    19201 tcatatcttt tgcggtatca accggattcg gactgatctt cgcgcttggc gtaggcggag
    19261 cggatatgcc gattaccata tctcttttga atagcctatc aggagtcgcc gcgtcaatat
    19321 ccggcttggc tatcggagat gctttgctag ttgcggtcgg cggaattgtc ggcgcatctg
    19381 gcttgctttt gacacaaatc atgtgcaaag ccatgaacag aaaattgttc gatatcttgt
    19441 tcggaggtgc taagcataca ccgcaagtca aacaaaatgt tgaaaaaacc cctgaaccag
    19501 ataaagaaaa agtcgaggaa aaagaaacgt tggatccggt ctctttggcc aaaaccgcca
    19561 aagatgtcat cattgttccg gggtacggaa tggctattgc ccaggctcaa caccttgtta
    19621 agcaactggc ggattatttc agagataaca atgcaagagt aagatatgcc atccatccgg
    19681 tggcaggaag aatgccgggg cacatgaatg tgcttttggc ggaagccgat gtggattatg
    19741 acgatctata tgaaatggat agcttgaacg atgactttaa aactgcggat ctggccgttg
    19801 taattggcgc caatgatgtc atgaacccgg ccgcgagaga agcggaaggg actccgattt
    19861 acgggatgcc gatacttaat gttgaccagt gcccgaacgt atttgttttc aattatgatt
    19921 tgaaaccggg atattcaggc gtcgagaacc ccttatattc tcgcaaagat ggtttatatc
    19981 tttttcttgg aaacgcttcg gagacactca aagattttct taacaaatta taaaatgatt
    20041 tattgccccg cttgaagcgg ggcttttttt atgtaatgtt taaaaaaatc tatttttatt
    20101 agtaaacatc aatttatttt cacaaaacca ggttataatg tgtttggaga aagaagatga
    20161 tatcatgaaa tgcctcgtta ccggagcgac cggacacatc ggaaacgttc tggttaaaaa
    20221 cctgtatgaa aaccattatc aggtgacagc cttggttttg cccaaggact ccgtccggat
    20281 gatcgaacct tattgcgaca ttgtggaagg taatgttctg gaccgcgatt ccatgttcga
    20341 tctagtcaag ggatatgatg tcgtattcca tttggccggc attgtggaaa tcggttctgg
    20401 caaaaagaaa accctaatgg cagtcaattt catgggaacg aaaaacgtat tcgacgcctg
    20461 cgttgtcaac aacatcggca agtttgtata tacctccagt gtccacgcga tcgaagaaaa
    20521 accttttgga gaaaccatca cagaaaccaa gatttttgat cctgccagag tgcatggatt
    20581 atacggaaaa agcaaagcca aagcctcaga ctacgtcatg acccattatt cgggaaacac
    20641 aaaggtgatc gtcgtcatgc cttcaggagt gatcgggccg tacgattacc agcgttccaa
    20701 catcagtcag atgtttgtcg atatcctttt aggacatctg acggcatatg tcaaaggcgg
    20761 atacaatttt gtcgatgtca gggacgtcgc caacggaatc aggcaggcga tggaaaaagg
    20821 gaaagaccat gaaacatatt tgctgacagg taacgaaata accgtcaaag aactattgga
    20881 taaaacgacc aattatattg ggaggaagcc gatcaagaca aaagttgccg cctggtttct
    20941 tctggccgtc agttatttgg ccgaattcta ttacatcgtt ttgagacaaa aaccactttt
    21001 cactcattat tctattgtcg tgttacggtc gaattacgcc ttctccaacg agaaagcaaa
    21061 acaggaactc ggatacaccg tacgcggaat cgatgaatcg ataaaggatt ccatcgattt
    21121 tgtcaaaaat aattttgtgg agaaaaaggg taaaaaattc aaaaagaaaa tcattgccaa
    21181 ttgaaacaag ccatccggaa tttccggatg gttttctttt tgcctgggca ttatctcgat
    21241 ttgtgttgta taatgtcttt tggtggtaac atgaaagaaa tccgggcaag cgtgaaagat
    21301 atcgccgaat acctttatgg cggcggcgat cttgccaacg acaggctgat ggccattaga
    21361 caacaagaag gcatcagcat ccatcagtac tggcagtcgc tttacaagga aaacgaccag
    21421 aaagaagtat atgtcgcaag tgaagataaa accggcgaca tttttctaat tgtctccggc
    21481 cggatcgacg gcatcgtcac gagggacgaa gaactaatgc tggaggagat aaaatccacc
    21541 cgccaggaac cggccgcaat cgacgagact acctatcccg cccacctggc ccaagcaaag
    21601 atctacgcgc acctttatgc cagagaaaac aagttgaaaa gactgacggt cgttctgaca
    21661 tatgtcaatg tagacacaaa agaaagcaat cagatcgaga aaaattattc tgccaagatt
    21721 ttggagaatt attataacaa agccattcaa aaatacatcg agtgggtcat gctcttggaa
    21781 gagcacgaac aaaaacgcgc caaatcgatc gaaggattga attttccgtt tgattcatac
    21841 cgacccagac aacgcgacat gatggcatcg gtataccgga acatcctgga aaagggaacg
    21901 ctttatctga ctgcccctac cggtatcggc aagacgatcg gaacgatctt tccggccttg
    21961 aaagccatca acgagccgag acaaaaggtt ttttacctga cagccaaaaa tgacgggaag
    22021 caaatcgctc ttgatacagt atcccttttg gaaaccagcg ggcttcacgc caaaacctgc
    22081 gaattaactg ccaaggaccg gatgtgtttt ctgaaagaac gcgactgtga tccggagaat
    22141 tgtaaatacg ccagaggata ttacaaaaaa atatacaagg caatccgcga tgcatattcc
    22201 aacgccaccc tttttacgag cggcgtaatc aaaagttttg ctagaaaaca cgttgtctgt
    22261 cccttcgaaa tgtcactgga tctatcgaat tattgcgata tcgtcatttg cgactacaat
    22321 tatgttttcg atcctcgggt gagactgatg cgttatttcg aggaaagatc cgctgatccg
    22381 atccttctga tcgatgaagc ccataatttg gtacaaagaa gtcgggaaat gtacagcgcc
    22441 accgttacct ggcagatgtt ccgggatctg gaaaagcttt gtcagaatgt caagccatca
    22501 ccaaaacgag aaatctcccg gatcgtggat ttgtttcaag aactggaact cgaactcatc
    22561 gaagtcgatt ttgtcaaata cgaggaacaa aacgagtatc tcatccagct tttaaagaaa
    22621 cttttgttaa gactggatca aatcctgttt ggagaaaaaa aagtaccgaa ccgcagccaa
    22681 ataatcgaag cttatttttc catctcgcag tttgtcagga tcagcgagtt ctacaatcag
    22741 gaattcgtct tcttgctgga aaagcaaaat gacgatgtct ttgtttccat caaatgtcta
    22801 aacgcttccg gattcataaa taaaaccatc ggcgaatacg cgctgtcggc tctttttttc
    22861 agcgcaactc tagaaccgat cgattattac aaaacccttc tgacggaagg aaaaggaaac
    22921 gattccgaat atccgtcccc attcaaaaaa gataatttac tggtattgat aggggagcac
    22981 gtgtccacaa gatatcagga cagaaaagat tctgtcagcg agatcgcttt ggccacgaaa
    23041 gctctcattt caggtaaaaa agggaattac atcgtgttct ttccttccta cgaatatctg
    23101 aaaatggtag aagagcgact ggatttcgat tcatcatcca cagacataat catccagaca
    23161 aggcaaatga gccaggagga acgttcttcc atggtgatgc ttttcaaaac gagaagcgaa
    23221 aaaacccagg tgggtctctt tgtaatgggc ggtgttttcg gagaatcgat agacttgatc
    23281 ggagatatgt tgtcgggagt tttggttgtc ggagtcggtt tgcccgctct ttccccgtac
    23341 aacaatatcc ttcgcagcca tttcgatctt gagttcggac aaggattcga ttttgcttac
    23401 acgtatcccg gcctaaacaa agtgattcag gcggtcggcc gggtcatccg gaccgaaacc
    23461 gaccggggag ttgcgatttt aatggatgac aggttcgcat cgaatcggta cataaaattg
    23521 tatccgaggc aatgggacaa agcggaaata tgcggcaaca cgaaagaaat agaagaaaga
    23581 atcgccagat tctggcaaaa aaaggaaaaa taacaaaaaa aggcggatag caggaaaaaa
    23641 aacttgcttt ccgccttaaa atttgataat atataaaggc ctgtgacgaa caggaaggca
    23701 gtgatttgta aggttgctga cacacacata ggcgttgtta tgacaccaaa agtgcgaggt
    23761 caggtaattt acaatgagcc tgcgtataag cgaattatat gcataaggag gagccacaat
    23821 ggccgcaaac caagtaatca gaatcagact caaatcctac gatcacagat tgttggatca
    23881 atcggcgaga aagatcgtcg acacagcgaa gaaaaccgga gcggtagttt ccggtccgat
    23941 cccccttccg acggagaagg agatcttcac tattctgaga agcccgcatg ttaacaagga
    24001 ttctcgcgag cagtttgaaa gacgtacgca caagcgtctg atcgacatca tcaatccatc
    24061 cgcaaaaacg atcgactcgt tgatgcatct ggatctgccg tccggtgtcg acattgtatt
    24121 gaagtaagga aaatccaaat aggaggtata ctcatggcaa aaggtatctt aggaagaaaa
    24181 gtgggaatga cccaggtttt cgacgaaaac ggtcacctgg tcccggtgac ggtagttgtc
    24241 gtcaccccga atgtcgtcct gcaaaagaaa acagtcgcca ctgacggata cgaagcggtc
    24301 caactcgggt tcatggagaa acgtgcaaat ctcgtgaaca aaccgctaac cggacacttc
    24361 gccaaggtca acacgacacc taagcgctac gtaaaagaga ttcgtcttcc ggaaatgatg
    24421 gaatacgaag tcggccaaga ggtcaaatgt gatatatttg cccctggtga ttatgtagac
    24481 gttacaggaa cttcaaaggg caaaggttat caaggcgtaa tcgtccgcca caatttcagc
    24541 accggcccga tgaaacacgg atccaagtat catcgtggag tcggctcgat gggctcgatt
    24601 gccgccaacc gcatcttaaa gggcaaagca atgcctggac ggatgggaca cgaaacggtc
    24661 accatccaga acctgcagat cgtccgtgcg gatgttgaaa acagcatcct gctggtcaag
    24721 ggcagcgttc ccggaccgga caaatcgctt gtcatagtca aaaatgcaac aaagaagcag
    24781 aaataggaat atcgctgaaa ggagaaaaat gtcatgccca ctataaaatt aatgaatcag
    24841 gcgggagaag ccgtcggcaa catcgatctt tcagaagcgg tgttcggagc tgaacccaac
    24901 cagcagataa catacgaagt ggtcaaagcc cagagagctg ccatgcgcca aggcaatgcc
    24961 agcactaaaa accggtcaat ggttcggggc ggcggccgca aaccgtatcg tcagaaaggg
    25021 acaggacacg cccgtcaggg aacgatccgt gccccgcagt tcgtcggagg aggtaccgtt
    25081 ttcggtccgt ccccccgcag ttacagttac aaggtcaaca agaagatccg ccgtcaggcc
    25141 ctccgcattt ctctttccga caaggtgaga gaggaaagtc ttgtcgtcgt cgacaagatc
    25201 acccttgagt cattcaagac aaaaggcgtc gtcgagatcc ttgcgaacct caaacttgaa
    25261 ggcaagatca tggttgtcct cgacaccatc gacgagacct tcgacatcgc gtcgagaaac
    25321 ctgccgaatg tcatcgcctt cgcaagcgat cacatcagcg tctatgacgt gatgaattcg
    25381 gccaaagtgc tcgccaccga ggctgcgatc aagaaaatag aggaggtact cggctgatgg
    25441 ataaatatca gattatcaaa agcccggtag tttcggaaaa atcaaccaag ttggcggaac
    25501 aacgcaaata tacattcgaa gttgacaaaa acgccaacaa aattcagatc aaacaggcga
    25561 ttgaagaaat cttcgacgtc aaagtaacga aagtaaatgt cgtaaactgc atccccaaga
    25621 ccaagaaggt cggcaaattc gaaggattga aacctgccgt cagcaaagct gtggtcactt
    25681 tggcggaagg ttacaagatc gacatctaca cggcataggc gggaggaata actaatggct
    25741 attatcaaat tcaaaccgca gaccaacggt cagcggaaca aaacgaagct ggattacgcc
    25801 gaaatcacaa cttccaagcc agaaaaatcc ttgactgttt cgcttaagaa agaagctggc
    25861 agaaacaacc aaggaaaaat caccgtccgt catcaggccg gcggcgtcag gcaaaaatat
    25921 cggatcatcg attacaaacg caacaaagac ggaatccccg gaaaagtcgc gacgatcgaa
    25981 tacgatccta atcgcagcgc caacatcgcc ctgatcaatt atgccgacgg cgagaagaga
    26041 tatattctcg cccccaaagg attggaagtc ggatccacca tcgtttccgg aaaatcagtc
    26101 gacatcgtca ctggaaacgc gatggagatc atgagcatcc ctgtcggtac cgtcatccac
    26161 aatatcgaac tatatcccgg tcgcggcgga cagttaatca gagccgccgg caccagcgcc
    26221 cagattctgg gccgcgaaga aaaatacgtt cttatccgtc tcaaatccgg agaagtcagg
    26281 agaattctcg gagtatgccg cgctaccatc ggtgaagtcg gcaacgaatt ccatgaactc
    26341 gtgaaactcg gcaaagccgg acgtaagcgc catatgggta ttaagccgac agtccgtggt
    26401 tccgtcatga acccggtcga ccacccgcac ggcggcggcg aaggcagaca gccgatcggt
    26461 catccgtctc cgctttcacc ctggggcaaa ccaaccctcg gtaaaaagac gagaaagaac
    26521 aacaaggcca gcgacaaact gattgtcaga cgccgtaaca aataaggaag gagaaagtct
    26581 aaatgtcacg ttcaatcaaa aaaggaccct tctgcgacga ccatttgatg aaaaaggtca
    26641 ccgaagccaa caagtccaat tcgaaaaaag tcattcaaac atggtccaga cgttcaacca
    26701 tctttccgca atttgtcggt ttgacgatcg gtgtccacaa tggtcgtgaa cacattcccg
    26761 tgtacatcac cgaagacatg gtcggacaca aactcggtga attttcaccg acgagaacat
    26821 tccgcggcca tgcggataaa aaagccgtca agaaatcagg aggagcacag taatggaagc
    26881 caaagaagcc aaagccgttc tccgttcagt cagaatcgct ccccgcaagg tgcgtttggt
    26941 agttgacctg gttcgcggcc aaagtatcaa tgatgcatac gctattctga gaaaccttca
    27001 gagaggcgcc agcaaaccgg tggaaaaact gatcaaatca gccgccgcca acgccgacca
    27061 caatttccaa ttagacccag agaaactgta cataaaggaa atctttgtcg gagaaggaaa
    27121 aacattcaaa agaatccagc cccgctcacg cggtcgcgca ttcgctatct tgaaacgcac
    27181 gagccacatt tacgtaaccg tggcggaaag ggaataagga ggagtttatg ggacaaaaag
    27241 tcagtccgat cggacaacgc ataggcataa ttctcgattg ggaatcccgt tggtacgccg
    27301 acaagcgtga cgtggcagac ctcttgatcg aagacatgaa gatccgcgag ctccttaacg
    27361 aactttacaa gaacgccagc gtttctaaga tagtcatcga aagaagcaaa tcccgggtta
    27421 tcatcactgt caacaccgcc aagcccggaa tggtcatcgg ccgcaacggc gagaccaaga
    27481 acgcagtagt tgacaaactc aaaaaactca caggcaagga agtcttcctg aatgtcgttg
    27541 agatcaaacg cgccgaactg gacgccaaac tggtcgccga cagcatcgcc cggcaattgg
    27601 aaaaccgcgc atcgttccgc cgggttcaga aagtcgccat ccaaagagcc ttgaaagccg
    27661 gagccaaagg ctgccgcaca ctcgtttccg gacgtttggc tggcgccgaa atcgcccgcg
    27721 ccgaaggata cagcgaagga aatgttccct tgcataccct gagagcggat attgattacg
    27781 ctttggccga agccaagacg acatacggaa aactcggtgt caaggtatgg atctacaagg
    27841 gtgagaaact gcctcagaag aaggaggcca gataaaccat gttgatgcca aaaagaacta
    27901 aatatcggcg tccccatcgg gtaagatacg agggacacgc caaaggcgga accgaaataa
    27961 ctttcggtga atatggactc atggcccttg aaggtgcatg gatctccgct cgccagatcg
    28021 aagccagccg tgttgccatg acgcgtttta tgaaacgcgc cggacaagtc tgggttaaga
    28081 tcttcccgca tatggccaaa accaaaaagc cgcttgaagt acgtatggga tcaggaaaag
    28141 gcgccccgga agattgggta gccgtcgtca aggaaggcct gatcatgttc gaaatcgccg
    28201 gcgttcctga agatgtggcc aaagaggcct tgcgtctggc atcgcacaaa ttgccgatca
    28261 agaccaaatt tgtgaagaag gaatgtggtg acgaaaatgg cacatgttag tcaaattcgc
    28321 gaaatggaaa ccgacgccat tctaaaagaa atcgataatc taaagaaaga gatgttcgat
    28381 cttcgcttca aacaagcgac cggacaactc gaaaatacag cgcgcctgca tgtggtccgt
    28441 aagaccatcg cccagatgaa aacaatcatc tccgagcggg aacgcgagta gggaggataa
    28501 ccatggaaga gaaaaaactg catcgcgtct ttacgggaac cgtggtttcc gccaagaacg
    28561 acaagacaat caccatattg gtcacgtctt acaaaaaaca tcctctatat aacaaacgcg
    28621 tgatctcatc gaaaaaattc accgcccatg acgagaagaa cgaagccaaa gaaggcgacg
    28681 tcgtgagaat caccgaatgt cgcccgctct cgcgtacgaa aaaattccgc ttgatcgaaa
    28741 tcgtcgagca caaagcggaa tagcggcggg aaagcgagga cactgaaatg atccaacagg
    28801 aaacaagact gaaaatagcc gacaactccg gtgctaaaga aattcttacc attaaggtgc
    28861 tgggcggcac cagccggaaa tacgccaaca tcggcgacat tatcgtcgcc acggtcaagt
    28921 cggccacgcc tggcggtttg gtcaaaaaag gcgaaatcgt caaagctgta atcgtcagaa
    28981 caaaaagagg actccaacgt cccgacggaa cccacatcaa attcgacgac aacgccgccg
    29041 tcatcatcaa agatgacaaa agcccgaaag gcacccgcat tttcggaccg gtggcccgcg
    29101 agctcaggga tgcagaattc accaagatcg tatctctcgc tccggaagta ctgtaaggga
    29161 ggatgccatt catgaaattt aagaaaggcg ataaagtcgt catcatagct ggtcaggaca
    29221 aaggcaaaaa aggaaccatc agcaaaattc tgaccaaatc ggaacgggtc acgctggaag
    29281 gcgaaggcct ggccaaagtc aagaaacatc tgaaaccgtc ccagatcgct cccgacgggg
    29341 gcatcatgga cgtcgacaaa ccgatccacg tttccaacat catgttgctt gatccgaaag
    29401 aaaagaaacg gacaaggatc ggttacaagg aagtaacccg gaaaacgaag aaacaacagg
    29461 atgagaccga aacggtcaga tatgccaaaa aatccgggga agtcctggaa tgatggaggg
    29521 agaatataga tgaaccgcct acaggaaaaa tataaaagcg agattcttcc cgcgttgact
    29581 gaaaaattca aatattcttc cgtcatgcaa tgtccgaaag tcagcaagat cacgttgaac
    29641 ataggtgccg gagacgccgt gcagaatcca aaagttctgg acgacgcggt cgccgaactc
    29701 acccagatca ccggacaaaa accgattatt acaaaagcaa agaaatccat agcaaacttc
    29761 aaactccgcg aaggcatgcc gattggctgc aaagtaacgc ttcgcgggga aagaatgtac
    29821 gatttccttg ataagctgat cacaatcgcc ttaccgcgcg tccgcgactt ccgcggtatc
    29881 aacccgagag gattcgatgg acgcggcaac tacacgttag gtatccgcga acaactgatc
    29941 tttccggaga tcaactatga caaagtgagc aaaatccggg gaatggacat cgtcatcgtc
    30001 acgacagcga aaacggacca ggagtccatc gagctgctga agatgttcgg catgccgttc
    30061 agaaagaatt agggaggcgc aatcatggct aaaacatcga tgaaagtcag acaacaacgc
    30121 ggatcgaaat tcccggttcg caattatacg agatgtgagc gttgcggacg cccccatgcg
    30181 gtttaccgca aattcaaact ttgccgcgtt tgcttccgtg aactggccta caaaggacag
    30241 attcccggtg tcaagaaggc aagttggtaa agattatgat cagattcgat tcctatttat
    30301 caaggaagga ggcatttaga acatggttat gacagatccg attgcggata tgcttacgag
    30361 aatccgcaat gccaatcaga tgaaacacaa aacagtcgac gttcccgcct caaaactcaa
    30421 agccgagatc cttaatgtct tgaaaaacga agggtatatc accgattacg aattcgtagg
    30481 tggttcggtc caaggcaatt tgaaggtaac gttgaaatat ttgcccaacg acgcaagagc
    30541 ggtcagaggg cttaaaaaga tttccaagcc cggactcagg gtttatgcca aatccgacga
    30601 tctcccgagg gtgttgaacg gtttgggcat cgccattgtt tccacttccc aagggatcat
    30661 gacagaccgg gaagccagaa ggaaacaagt cggcggagaa gtcctggcat acgtatggta
    30721 atgccgcaaa taatggatat catggaggtg caagaataat atgagtcgta taggaaagct
    30781 accgatcgtg attcctgccg gcgtttccat aacggttgac aaggacaacc atgtcaccgt
    30841 caagggcccg aaaggagttt tggaattcac tttttccaaa gacatttcga tcgctactga
    30901 aaataacgaa atcaccgtaa cgagaccatc tgactcgctc atccaccgtg aacttcacgg
    30961 aaccacgaga gcggtcctaa acaacatggt ggtaggcgta acggaaggat ataccaagat
    31021 actggacatc aagggtgtcg gatatcgtgc cgcgctcaaa ggttccaaca tattggttct
    31081 ttccgtcgga tattccaatc cggtggaaat cgccattccc gagaatctta cgatcgaagt
    31141 gccgtcgaac acggagattt tcatcaaggg aatcgacaaa caagctgtcg gagaattcgc
    31201 agccgtgatc cgcaaggtcc gcgctcctga accatatcag ggcaaaggca tccggtaccg
    31261 tggcgaacat atccgccgca aggaaggaaa gacggccaaa taaggcccgg ggtgaaaaac
    31321 tatgtttaaa ccagttaata agaatgttca gcgcgagaaa agacactggc gtattcggac
    31381 aatcatcaaa ggcacagctt ccagacctcg gctaaacgtc tatcgttcca acaaagccat
    31441 ttactgccag atcatcgacg atgacctgca aaccaccttg ctggcagctt catcgcatga
    31501 agtcaaggat gtcaacggcg gaaacgtcga aggcgccaag gcggtcggaa ccctgatcgc
    31561 caagaaagca ttggaaaaag ggatcaagga agtcgtgttc gaccgtggcg gttacctgta
    31621 ccacggaaga gtgaaggcac tcgccgacgc tgctagagca gcagggttgg aattctaatg
    31681 gaggagggta gcccgatggc agaaaatatc gaaaaagacg gcaacaaaga aagagaaagc
    31741 cgggacaaca gaggcagacg gccgtcaaac cgccgggaca atcgcggcgg acaacaaaga
    31801 aaaaaagaag aaaaactcta cgaagaaagg gttgtcaata tcggaagggt gacgaaagtc
    31861 gtcaaaggcg gaagacgttt cagtttctcc gctttggtcg tcatcggcga ccgcaagggc
    31921 aaagtcggtt ttggaaccgg aaaagccagt gaagttcccg atgccatcca aaaggcgatc
    31981 gaagacgcca agaagaacat gtttgtgatt cccatcaatg gttcgacgat ctaccacaca
    32041 gtgaccggaa cttacggcgc agccaaagta ttcctgcgtc cggccgttgc cggtaccggc
    32101 gtaattgccg gaggtgctgt gcgtgctgtt cttgaacttg ccggagtcga agacgtcctt
    32161 tccaaatccc tgggctcgac atcgccgatc aacatcgtca gagcgacgat ggatggtctt
    32221 ttgtccacga gaagtattca agaagtggcc gaaacgcgcg gacttacgcc cgaggaggtc
    32281 ctgaaataat ggccaaaata aaaatcaccc tcgtgaaggg actggtcgga agaaaaccga
    32341 accaggttaa aaccgtcaaa gccttgggcc ttaagaaacg caattcctca gtggttaaag
    32401 aagacaacga tgccatcaga ggaatgatca acacagtggc ccatttggtc aagaccgaag
    32461 aagcgaagta ggaggcggag gcaatgaaac tcaacgaact caaacccgtt cagggcgcca
    32521 ctcattactc gaagcgcctc ggaagaggta ccagcagcgg caccggcaaa acagccggcc
    32581 gcggccaaaa gggccagaat tcccgttccg gaggcggaac gagacttggt ttcgaaggcg
    32641 gacagacacc actttacaag cgtcttccga aacgcggatt caccaacttc ttccgcaagg
    32701 aatacgcaat cgtcaatctt gcaaccctga acaagtataa aaacaaatcc gttgtcacgc
    32761 cggaaaagct gcttgaagac ggaatcttga gtgatctcaa ggacggagtt aaaattcttg
    32821 ccaacggcaa acttgaaaaa agtcttaccg tcaaggccaa caagttctcg gccgcggcgg
    32881 aagaagcgat caaggcggcc ggcggtaaaa cagaggtgat ctaggttgga aggcttgaag
    32941 aagattttca agaacaagga catcctgaaa cgtatcggat tcactttact tgtgtttttc
    33001 gtattcaaac ttctgacgta tgtcaccatg ccgcttgtgg acaaaagcgc attgtcaagc
    33061 ctttttgaaa acagcggatt cttaggcatc atcaactcca tttccggtga tgctttgcag
    33121 aactattcga tcgtcgcttt gggtattacc ccatacatta ccgcttcgat catagtccag
    33181 ttgttgcaga tggacatcat cccggccctc aaagagtggt ccgaggaagg tgaagtcggc
    33241 cggcagaaaa ccgcccgttt gacgagatat ctcgccatcg gactcgcttt tgtccaagcc
    33301 gttgccatga ctttcgcttt caacagcgcc accacacaga cgacgatttt caaaccgggc
    33361 gtcgtagcgt gggcgagcga agacaaccgc aacctttggt ttttggttta cttctatatc
    33421 gcgatcgttt tgacagccgg aaccgccttt atgatctggc tggccgatca gattacctta
    33481 agaggagtcg gcaacggttc ttcaatgctg atcgtcgccg gtatcatcat ttctttcccg
    33541 gggaccatcg tatctttgat ccaatattac attacagatc cgagcggagc catgacaacg
    33601 gtttccgcaa gccagggaac cctggttggg tatattttgt ttgccgtttg tattctgatg
    33661 ttcgtcattg tacttatcgg tattacttat atgcaggatg cgaccaggaa agttccgatc
    33721 cagtatgcca accggccggc cggatcgaaa ttctccggca aatcggaatc aaacattccg
    33781 atcaagatta atacggcgaa cgttatcccg gttattttcg cagccatcat cctctctttg
    33841 ccaaacacga ttttcaatta catcacgctt ggagacacgg cttcgaattg gatgggggaa
    33901 attttctcct ataaccaacc gatcggattc gccatctaca tcatcttgat cttcattttc
    33961 acgttcttct acacgttcat cctgatcaaa ccggaacaaa ccgccgaaaa tctgcagaaa
    34021 cagaatgctt acatacccgg tgtgcgtccc ggaaccgaga cggaagcata catcacgaag
    34081 acattattca gagtaaccgt cgtcggagcg ctttatctgg tcatgattgc cagtttgcct
    34141 atcatcacat caatggcact cgggcactca tacgcccaaa tcggcggaac aagtttgctg
    34201 atcatcgttg gtgtcgcctt ggaaaccgcc aagcaaatcg aaactgacac gaaagacaag
    34261 gcttacgccg gctttctcag ataacctaag catcatgact aacctgttga ttatggggaa
    34321 gccgggagcc ggcaaaggca cattggccgc cgtaatactg gaaaactacc atctgaaaca
    34381 tatttcgacc ggggatatct atcgcgagga aatgcagaaa gaatccccga tcggcttgtt
    34441 ggcgaaatcc tacatcaacg ggggcaaact ggtgccagac gacattacca acgaaattgt
    34501 cgagaatgtt ttaaaaaaca ccaaagaata tcccaacgga ttcatgttgg acggataccc
    34561 cagaaccatc agccaggccg ggacgttgga taaaatgcta accgacctga atctcaagtt
    34621 ggacctggtg gtaaatgtcg atatcagcga acccgtcctg atgcgccgca tcaccggaag
    34681 acgcatttgt tccgtatgtc aggaaactta ccatatcgag aacaaaccgc ctaaagtccc
    34741 gggcatatgc gacgtttgcg ggggaactct aattcaacgg aaagatgaca ccgaagaagc
    34801 ggtggcgaca agactcgccg tctataacga aaagacgaaa ccgcttttgg aatactatca
    34861 ggacaaagaa atgctcttca cggtcaacgg cgaattaccg gccgaagtca tcttcaagga
    34921 tattgtaaaa agattagagg cggtttgatt gatcacaatt aaatcagaaa gggaagtcga
    34981 actgatgcgc gaagccggca agattgtggc gcttgcccac aaggcggcca aagaagcgat
    35041 tgcaccgggc gtatcgacca aaaagattga cgaaatcatc gaaaaagtca tccgtgatca
    35101 cgatgccact ccgtctttca aaaattacaa cggctttccc gcagcgtcat gcatttctgt
    35161 caacgaacat gtagttcacg gaataccttc gcgcgagatg atcctcaaag acggggatat
    35221 cgtttctgtc gacatcggtg cctgttacaa gggatatcac ggcgattccg cctggacata
    35281 tccctgtgga cacgtctccg aaagggtcat gaatttgttg gaaggcacgg aaaagagcct
    35341 atttgccggg ttaagccagg caaaggcggg caatcgtctg accgacattt cgaatgccgt
    35401 tcaaaagacg gccgagggct taggatacag tgtggtccgt gaatttgtcg gacatggcgt
    35461 cggacaaaaa cttcatgaag acccggcaat tccgaatttt ggacatccgg gcagagggcc
    35521 gcttttgcgt caaggcatga ccctggcgat cgaaccaatg atcaatctag gccggaaaga
    35581 agttaggatc cttccggatg gatggaccac gattacggcc gacggaaaac cgtccgccca
    35641 cttcgaacac acggttctta tcaaggaaga cggatacgaa atcctgacga aggtaaattg
    35701 ataggggtga ttcaatggca aaaaaagatg atgtcatcga aatgatcggc acagttgtgg
    35761 aagttcttcc caacgcccaa ttcataatcg aactggaaat aggacatcgc atcatcggtc
    35821 acgtttctgg taaaatccgc atgcattaca tacgcatttt accaggggac aaagtcaagg
    35881 tcgaactttc gacctatgat ttaacacgtg gtcgcatcac gtatcgcatg cattgatatt
    35941 taggaggaaa cgatatgaaa gtcagaccat cagtaaaaaa aatctgcgaa aaatgcaaaa
    36001 tcatcaaacg ccatggcaat gtcatggtga tttgtgaaaa cccgaaacac aaacaaaggc
    36061 aaggaaagta ggaggaaaca atggcacgta ttgcaggagt agacatcccc agagaaaaaa
    36121 gagtcgtcat ttccttgacg tacatttacg gaatcggaaa aacgacttcg gaaaagattc
    36181 tggccgcggc cggagtttcc attgacaaac gcgtcaaaga cctgacagac gacgaagtcg
    36241 taagaattcg tcaggaagtg caaaaacata aagtcgaagg cgatttgcgc cgtgaagtcg
    36301 cgcttaacat caagcgtctg atggaaatcg gctgctacag aggcatccgc catcgcaaag
    36361 gtttgcccgt ccgcggacaa aacaccagaa acaatgccag gacacgcaag ggcccgagaa
    36421 aaaccgttgc gaacaagaag aaataaggag gtatggagta aatggcacgc aatgtaacga
    36481 aaaagcgtcg ggtgaaaaag aatattcccg tgggagtagc ccatatccac tcgactttca
    36541 acaataccat catcaccatc accgatccga ccggaaacgc catcgcctgg agttcagccg
    36601 gagcaatcgg atacaaagga tcccgcaaat cgactccgtt cgcagccgga ttggccagcg
    36661 aagcggctgc caaagcagcc atggaaaacg gtgttcaaaa ggtcgaagtt tccgtcaaag
    36721 gcccagggcc cggacgcgaa gccgccatca gaagcctgca ggtagcgggt ctcgagatca
    36781 ccgccatcag agacgtaaca ccggttcccc ataacggctg ccgcccgccg aagcgcccgc
    36841 gcggctgata ggaggcagga cctatgaaag aacttaagtt cgaaaaaccg aaaacgacga
    36901 tcgaagagat aaacgacacc tacggcaggt tcgtcgtcac cccgctcgat cgcggattcg
    36961 gcatcacgat cggcaactcc cttcgccggg tcctcttgtc ttcacttccg ggagccgcca
    37021 tcatcaacgt tcaaatcgaa ggtgtccagc atgaattcac ggccatcgag aatgtcacgg
    37081 aagatgtcac gaccatcatc cttaatctga agggtgtcgt cctgacgatc gattcgtcca
    37141 acccgaacgt ggaaaaaaga atggaagtca tcgtcgacgg accaaccgat gtgttcgcaa
    37201 aagacatcat cgcagacgac gaagtcgccg tcatcaatcc ggaccactat atttgtcatg
    37261 tgaacaaagg tgcgcggttc cggatggttc tccatgcgag aaaaggcaac ggctatatca
    37321 gcgccgacaa gaacgcggtt tatgacaata tggtcggagt cattccgatc gacagtctct
    37381 acactccggt cgtaaaagcc aactatattg ttgacaaaac ccgcgtggac gacagcgccg
    37441 atcacgacaa actgaccgtc gaagtctgga ccaacggttc gataagtcca aaggaagcca
    37501 tcggcatggc ggccaaaatg ctgatagatc atttctattc gctggtcgaa ctgtcatcca
    37561 aggcgatgtc ggaagatttc atgatcgaga gaaaatccga ggaagcatcc cgcaatctgg
    37621 aaaagccgat cgaagatctc gatttgtcgg tcagaagtta caactgtctg aaacgggccg
    37681 gcatccatac attgggagaa ctcgtcgaaa aaaccgagga agacatgatg aaagtccgca
    37741 acctcggaaa gaaatcgctc aaagaagtaa aacaaaaact tgaagaactc aatctgagtt
    37801 tggcaaaaca ctgattggag gaggataatc atggccagag gatatagcaa actgcatcgt
    37861 agaagtgaca atcgcaaagc cctgatcagc gatctcgcga cccaactcat cttgcacggc
    37921 aagatcgaga catcgcttgt caaggccaaa gaagccagaa ggacagtgga aaagctcatc
    37981 acttacgcga aaaaaggtga tttgcactct ttgcgtttgg cggaagcgag acttcgcgac
    38041 ctgtcgatcg acaatcaaag tgctttgcag aaactgttca aagaattggg gcccaaatac
    38101 atggatagac aaggcggtta cacccggatc tacagaaccg gagaacgtct tggcgacgcc
    38161 tgccccatgg caatcatcga attggtttga tccaacacat gaaggcgccc gcaaggcgcc
    38221 ttttcttttc tttaaaatgt tgctaaaagt tcgcttaaat ggtatatttt tcatgtccga
    38281 tgacactatc gtgaggtgat tttatggaaa cggcgaaggt cgacattttc atggatacat
    38341 ttagaagttt agaaagctat ctgcgggtcg aatacgatcg tggcaactac cgcgaacaga
    38401 ctttcatgaa cactctcttc cggatccggg gcagacagga aaatcatatc atccaaaacc
    38461 cgcaatattt cgacattctc caacaagccg cccagttaag aaacgtcatt gcccataaca
    38521 atgacgttgc cgaaccgacg gatacgtttc taaaaaaatt ccagcgtatc accagaatga
    38581 tcgtaagccc cgttagggtt tcgaatgtta tgatcccgat gtcaagaatc aaaaaagcgt
    38641 cgaccaagga taaaatagct gatatcatgg atacgatgga cagatatgga tattctaaaa
    38701 tcccggtgtt ttccggtcaa cgccttttgg gtgtattcac aaagaatacc tttttttaca
    38761 aactattgac ggataaacca gcttcgatca accgcgacat gcggatggaa aattttctgg
    38821 atgttatcga tttcgacggt gatccgacca aatatttcga ttttatccgc aaggatgcgg
    38881 atattttcga agcattgacc agatttcgcc gaaatttccg ggaaaaaaat catctggaaa
    38941 tgctttttgt caccgaaaac ggaaagaagg aggaaaccat cctgggcatc gtcacgttgc
    39001 cggacctcga agaatcagtg gtcatttgaa aagtacggat ggtaagtacc gatgagtttt
    39061 ttggaaacca aagatctttc tttttcctat gaaaacgaca aaccggccct tgacaaaata
    39121 aatcttaagg tcgaaaaagg cgaatggatc gccattctcg gtcccaacgg atccggaaaa
    39181 tcgacgctgg caaaagccgt cgtcggtctt ttggaaaccg aaaaagggac cgtttttgtg
    39241 gatggcaatg aactgaacga gaaaaccgtc tacgaagcca gacaaaagat cggtattgtt
    39301 ttccaaaatc ccgataacca attcgttggc gttaccgtca gggacgatat cgctttcggc
    39361 atggaaaatc tttgtcttcc gagagaagaa attctgaaaa gaatcgattt tttcgcaaac
    39421 aaggttggta tcaccgactt gctcgacaaa gagcccgcca atctgtccgg cggtcagaaa
    39481 cagagagtcg cgatcaccgg cattctggcg atgaaaaccc aggttatgat ttttgacgaa
    39541 gccacatcga tgctcgatcc ctatgcccgc gccgatctca tggagtatat ccgatctttg
    39601 cacaacgaag gcataacgat catcatgatc acccacgaca tcgaagaggc gttgtgggcg
    39661 gaacgacttg tcgtcatgaa ggaaggccgg atcgtgtccg acggtaaaaa agaggatctt
    39721 ttgaaaagcc gcgatatgct tgccggggct tttcttgaat taccggtcgc attagaaatc
    39781 agtaatcttt taggtgatga cgaagaagca aaggcggtga aaactttcct atgggaattg
    39841 agttcaaaaa ggtaagctac cgctatatga aaggcaacgc cgcttttttc caggccatca
    39901 acaatatcag tctggaaatc gacaatctgg acgagttcat ctgtctggtc ggccagaccg
    39961 gttccggtaa atcgactttg gttcaacata tgaatgcgct gattttcccg tccgagggag
    40021 aagtggtaat cgatggtgtt gtgatcactt cgaaacgaga taagaaaatc gattacaaca
    40081 gtctgagaaa acatgtcgga ctggtttttc agtttcctga ataccagtta ttcgaggaaa
    40141 cggtggaaaa agatgtcatg ttcggaccgt tgaatttcaa gatgacgaaa gaagaagctg
    40201 ctaaagcggc aaaggtagcg ctcgaacagg tcggattaga cgatacttat cttgagagaa
    40261 gtcctctgaa tctgtccgga ggcgaaaaga aaagagtgtc gattgccgga attctcgccc
    40321 tgaatcccga tgttttagtt ctggatgagc caacgtccgg tcttgaccca gccggcaaac
    40381 gccggttgat ggaacttctt tcgtcaatta aaaaagaaac tggcaagacc attgttctca
    40441 tcacccacga tatggatcta gtttatcagt atgcttctag ggttttggtg ttaaatacag
    40501 gcaatttggt gtttgacggc accccggaca aacttttccg gcgcgatgat ctcgtaaaaa
    40561 accatcttga ctatcccgga acgatcaaga cgctgaaagc cgtcagggag aaatttggcg
    40621 tcgatatcga catttataaa aaaaccccgg aagaagccgc cagggaaata aggcgggtga
    40681 tggaaaagtg aaaggaatcg taatcgggca atatattccc ggaaccggat tcttttacaa
    40741 actggacccg cgtacaaaaa tcgccgccgt tgtcattttg atggtggccg tcttcttgct
    40801 cgagacaata cctcagcttt tgattgcctt gggattgtct attgttctgt tgttatgggg
    40861 aaaaatatcg gttggccggg ttcttcgtgg attgaaacca cttctagtac tgatgatttt
    40921 cacttttttc ttccaggtac ttttcaatac ggaaggaacc gtcatctggc agacaacatt
    40981 ctatttttcc gcttcgagca tgattgcttt gggaattttg acgtttatgt ggcgcatcat
    41041 tgccaaaaac atatccggat tcaaattcat cgtgtttgtt ctttatctgg ccttggcgat
    41101 attgaatttc cattatatgc catcatggta tcagttcggc tcatgggact tcatcattta
    41161 ttccaaagga ttagagatgt cggttttcgt tgtcgtcaga cttttgatca ttatcacatt
    41221 ttccaccgtt ttgacgctga cgaccaaacc gacggatctg actcagggac tggaaaaaat
    41281 gatgcgtcct ttgaaacata tcggcatcaa tagcgacgag ttcgccctga tcatttccat
    41341 tagtctcaga ttcataccga cgatttttga cgaagcgatg aaaatcatgg ccgctcaagc
    41401 cagtcgcgga gccgattttt cccaaggaaa acttaaggat aaagtgaaac agatcgtttc
    41461 tctgcttgtg ccgatgttca taatcgccat caaaagaagc gaagatttgg cggatgccat
    41521 ggagtcgcgg aatttcgttc ccggtaaacc gagaacaagg atttacgaac tggcatacaa
    41581 acgtcttgat ctgtttgccg gtcttttcat cgttctttgc ctggcaggat ccatcgtgct
    41641 caaggtgtta atatgaaaca gataatggga acagcggatt ttttggcgat gatatcaccg
    41701 gatgcaggaa tcaaaatcgg ccacgaaatc cagctgggaa gggaaaatgt ccaaaaaacc
    41761 attcttgcga acgaggacat ctttgaccga atcgcttttc atcaaccatt cgcccatgtg
    41821 gcgatgtatg gggaagatca gaccggacat cttgtgaaaa tccatcttca ggacggaatg
    41881 atcaaaagaa tccttgtaac cccaggcatc acggcccgaa aaataaaact taccgttgcc
    41941 tatgacggaa ccgaatttta cggatttcag tttcaaaaga aagaaagaac gatccaggga
    42001 gaactggaga aagccatatc ggaaatgaat ggtaataata caacctgcca cggggcatca
    42061 cgtacggaca gcggagtcca tgcctttgga caggttgtcg actttgaaac cgacagggat
    42121 tttacggaaa aaagatggct ttcatccctt aacaagattt tgccgaaaga catcatgatc
    42181 cgtcaagcgg agtttgcgcc aaaacttttc cattcacgct acgatgtatg gaaaaaagaa
    42241 taccggtata gtttgaatat cggtgatgcc gatcccaagt tcagacatac ggaatggcca
    42301 atcggtccgc tgaaagacat ggaaaaattg gcggacaatc tcaaaatgct tgtcgggacc
    42361 catgatttca cgagttttgc gacaggcgaa aaagaagaca tggtcaggac aatatacgaa
    42421 acaaacctcg taatacgtga caacaaagtc gagatagata ttctaggaga cggatttttg
    42481 catcatatgg tgagactgat agtgtgccaa ttggtggaaa tcgctaatgg ccgttctgat
    42541 acaagcatca gggaaatcat tatggaaaag tcgcggaaac atacggtgag aatggcgccg
    42601 cctaacgggc tctgtcttta caatatcgaa tattagccaa taaaaaagcc cggatcggta
    42661 aacgaatccg ggcttgccat ataaaccgta tgatttacca atttgtttcg acggggtttt
    42721 cctttgcgag tttggcgatt tgccttttga gaacgatctt tttgatgagt gctacgacaa
    42781 gcagaatgat aatgttgatg gaaacgatgg ttgctccgct cggaatcttg agcgaatatg
    42841 ccgcccaaag tccgaaaatg accgacagtt cggaaaacag caccgcgatg ccaagcgtgg
    42901 aacggtaact ccaccccagt ttcatgccgg ccgccaccgg aataatcatc attgacgata
    42961 ttagcaaaac tccggccgct tccaacatga cggaaacgac gatggacagg acgaccatga
    43021 agagaatctg gaaaatattc acgttgatgc cgagaaactt tgcacttgtt tcgtcaaagc
    43081 tgacagcgat gatctgacga ccgaaaacca gaaacagaac agccaccacg gctgttatcg
    43141 ccagaagcgc gtacatatag taattggtga ccgtcagaat ggaaccgaaa agataggtgt
    43201 aaagggaacc ggtggctttg gccttggaaa agaagacggc cgacaacgcg gtgccaaggc
    43261 taatgacgat taccattgat atttccttgt aacttttgta tgatctgcgg aacatttcga
    43321 tgagcaagcc tccgacgatc gagaagataa ggccaaagaa aagcggttca tcgaagaata
    43381 tggagattcc gatagtggac aggaaaaggc tgaaagagac gccgactaag gaaaaatggc
    43441 ccagggtgtc ggcgatgaac gatagccttc tcacaacgac gatcgacccg atcaaaggag
    43501 cgatgactcc gagaataaca ccggcggcca gcgcctttaa catgaaatca taattgaaaa
    43561 ggtcggtgaa aaaagacaat gtgtacatca ttccgcctcc tcaattgtga ttttctcgga
    43621 agtctggaaa tcataggaca tgtcttggta catggtgaga acatgtgtga aattcagtgg
    43681 tttaagatcg ctgttgtgag tcactaaaat aatcgttatg ccctgttgat tgagcatgtt
    43741 gacggtttgg tagaagtaag ccgtgttcat cttgtcgatc gaggcggtcg gttcgtccag
    43801 aatcaaaaca tccggctgat tcagcattgc cctggcgatg aacacccgct gcaactgtcc
    43861 tcccgaaaga ttgttcacat tttcgttgac gattcccagg attcctagct ggtcaagaag
    43921 tttgagtctg aaactttgtc cggtttcctt caaactgtat acggaaagca tttcgttgac
    43981 ggttatcggg aaatcgtaat tgaagtcctc gaatttttgg ttgacataac cgaaccttgt
    44041 ccatccggta aacgtattga tatcctcatg atcgaaaaaa atcattccgg tttttaccgg
    44101 attgtatcct aaaagacatt ttatcagcgt cgtcttgccg gaaccgttct tgcctctgac
    44161 aacgagataa tcacctggat ttatagtaag cgacaaaccg ctgatgacgc tccggtgtcc
    44221 gtaagcgaac gtcaaattgt gaatatctat ttgcatgagc ccaccttgct ttcttaggat
    44281 attgtatcat accgctgaat attaatgaaa gaatttatac aaaaaacagc cataatgtga
    44341 gataataaat aagtaaagcg ggtggatgga atgaaaggtc tttttatcac attcgaaggg
    44401 accgaaggtt cgggaaaaac ctcggtcatc agaaaagtcg gcgaatattt cgataaactc
    44461 ggatatccgg tttttgtcac aagggagcct ggaggcagcc ggatttccga actgatcaga
    44521 aacattatcc tgaacaagga acacacggag atggatccga gaacggaagc cctgttgttt
    44581 gcggcttcta ggagacaaca tttggtggag aggatccttc cggcgctgga agcgcataaa
    44641 atcgttctct gtgaccgttt tgtcgacagt tcgctgatct atcagggcgt cgccagaaac
    44701 cttggcatcg acgaagttat gtccataaac caatttgcga tcgaaaatca ttttcccgac
    44761 cgaacgatct atatcgatgt ccgtccggaa gtgggtctga gccgggtttt tgggactccg
    44821 aaccgcgaag ttaataggct tgatctggaa caagtggcgt ttcacaagaa aatatacgaa
    44881 ggctatcacg aacttatgaa gaaatatcca gaccgcattc gttcgatcaa cggcgaagcc
    44941 gacattgata ccgtcacaaa agcggcgatt gatcagatcg ccgaattgtt gtgaggatat
    45001 gatgagaaag atgcttgtca cctgggatga acttgccggc taccagccgt atgtcgcaag
    45061 aattctcggg aacagtctga aaaacagaag actagcgcat tcgtatatat tggaaggccc
    45121 gcttgggtcc aaacgtttag acacggcgat attgcttgct aaaaccttgc tgtgccagaa
    45181 tctgcaagct aacggaaatc cctgccaggc ctgtcacaac tgtcatcgga tcgatgacat
    45241 ggctcacccg aatctgttct atgtggaagc ggaaagcgaa cagatcagaa aaaaacaggt
    45301 attggaactt cttcaggagt tttcgcgtca gtcagtcgaa aagggtccgc ggatatatat
    45361 cgtcaacgaa gcggaaaaat tcaaccagga agccgccaac accttgctta aaacaatgga
    45421 ggagcctggg caggatctgt atcagattct gattacctcc caggtcaacg ctttgcttaa
    45481 aaccattgtt tcccgttcgc aggttttgca tttcacaccc ataaacaaga aacagatagt
    45541 gttggaactg gaaaaagaaa atttgtccca agcgatggtt gctggtatat cggaatatac
    45601 tgataatacc gataaagccc gagaaatggc cgcttcccag caaacatcag atatattgga
    45661 actaatgttt gaaattttca aaaggctaga gacaggcgaa aaatcggcag tcatggcctt
    45721 taaagaacaa agggatgcga ttcttcccga tccagaagcg atcgatttct tccttacgat
    45781 gatggttttg tttctaaaag atatgctgtc gcacaaaatt cattatcatt ccctgatcgt
    45841 ttttgaaagc gaaaaggaat tgatcgggaa acttgccgac aagaccgatc aggaaaaaat
    45901 cgaggcagcc atcgaccaag tgctggatct aaaatcgaca acgaaataca atatcaacag
    45961 ccagttggcg ttcgacgcct tgctggttga tttagaaaga ggttttgacc atggaatatc
    46021 aggtagtgcc ggttaagttc aatcgactgg gaaaacaata ttatttctcg accggaggaa
    46081 aaacagtcaa taaaggcgac aaggtcgtcg tcgaaaccgt cagggggatc gaactaggcg
    46141 aagccgcatc cgatgtcaag atggtttcgg aacaggaagt cgtcctgccg ctcaaaccga
    46201 tcataagggt tgccctcgaa gcggatatca aacaagatga gacaaacaaa gccgatgaga
    46261 tatttgtaat gaacaaagca tcggaactcg tcaagaaaaa cgaactggaa atgaaacttc
    46321 tgaattgcga atacacactc gacagatcca aactgataat ctatttcaac gctgacggac
    46381 gcgtcgattt tcgcgaactc gtcaaggatc ttgctaatga attccgtctg aggatcgaac
    46441 ttcgtcaggt gggagcgaga gacggcgcga aagtcttggg tggcatcgga ccatgcggtc
    46501 tgccgacttg ctgttcttct tttctcggcg aattcgaccc ggtttcaatc aaaatggcga
    46561 aaaatcagaa tttgtctctg aatcctagca acatatccgg actctgcggc aaattgcttt
    46621 gttgtatcca ttacgaagat gacaattatc gggaatacag gaaaatagca ccgaagatca
    46681 actctttcgt caaaacgccg gacggccggg gaaaagtcac gcaactcaac tttatctcca
    46741 tggaagccag ggtggatttt gatgatggat catcgagata ttataaagta tctgaattag
    46801 aattccggga aaaagctacc gaagaaaaca tcgacaacaa cggaaacgat ctgaaagatc
    46861 tggaagaatg aacgacgttt ttttgaagga cggggaaaaa accgctaacc tgatggctta
    46921 tgaaggcttg aaaatcgtcg aaagcgaaaa ggcattccgg atgtcgatcg aagctgtcct
    46981 gttggccaat tttgtttcgc tgaatcctcg gattgccaga attatggatt ttggaaccgg
    47041 aaccggagcg ataccactta tgctttcgca atggacaaag gcaaagatca ccggagtgga
    47101 gattcaatcc gaactttgtg aattggccgt ccgtagcgta aaacttaacg gacttgctca
    47161 acaaatcgaa atcgacatgg acgacattgc tgccgtccat cttcattacc ggccgtcttc
    47221 ttttgacata gtcgtttcca atcccccgtt ttttaccggc gaaggaaaca aaggaaacaa
    47281 cgataagatt ttggttgcca aacacgaaac ccagctttct ttttcaaccc tggcaaaaca
    47341 ggcgtcgaga atgttgaaag agggaggcaa gtttgatttt atccaccggg cggatagatt
    47401 acaggaaatc atattggttt tacagacgga aaaatttgca atcaagagaa tccggcttgt
    47461 ttattcaaag cccggcacgc cggcactctg gatattgatt gaggccgcca ataagggaaa
    47521 ccgggggagc ctgtcatttg ccgaaccgtt gtatgtttac gaccaaaatg gcaactatac
    47581 gccggaggtc agcggatact ttcatcgtcc gcgggaggta tgagcatgta tcgtcatagc
    47641 aattatcaac ccggaaaagc cacgctttat ctgataccga ctccgatcgg caatttggag
    47701 gacatcacat taagggcgct cagaacctta aaggaagtta acgcaatcta tgctgaggat
    47761 acgcgtaaca ccatgaaact tttgaatcat tacaagatta cgggcgaatt gaaaagtctt
    47821 cacgaacata acgaaaaaga agtgacaccc gatatcatcg aacgtttggc aagaggagaa
    47881 aacatcggaa tcgtcagcga tgccggcatg cctcttttca gcgatccggg atacgggctg
    47941 gtacaaaaag ccatgggagc cgggtacaat gtcgtttgtc ttccgggagc caatgccgct
    48001 acgaccggac ttctgatgtc gggaatcaat cctcatccct ttacgttcgt tggatttctc
    48061 gataccagaa aaagcaaaag aaaagcccgt ctcgaggaat taaggttccg gaaagaaacc
    48121 ttgattttct acgaagctcc gcatcgtatc aaagcgatgc ttgccgactt ggcggaaaca
    48181 tatccggaca ggatggtttg tgtttgccgc gaaataagca agaaattcga ggagattgtc
    48241 aagggaaccg ctgaggaact gtcacaaatc gaggattgga agggcgaaat ggtcgtcgtc
    48301 atggaaggcg cttatgacga tgattcaacg gttgacaagg attcgtattg ccaacaggtc
    48361 gaagctcttg tcaaagctgg aacgcgcttg tcggaagcgg cgaaaaaggt ttcggaaacg
    48421 accggagttt ccaaaaacgt catttatcag gaatacttgg cgaaaaccaa gaaacagtga
    48481 tataatattg tctcaatgaa acagcaagga gttggcattt atgcgtattg cctgtttgct
    48541 ggcggatggt ttcgaggaaa tagaagcgat cggtacgatc gatctgcttc gaagatgcga
    48601 tgtcatcatc gatctcgtcg gataccaaaa ccgggaattc gtcaccggag cgcacggcat
    48661 cgtgatcaaa accgaaacac cttatgagaa attggatccg gctttttacg acggtctgtt
    48721 aattcctggc ggcggaaaac aatccgctac cctaagaagc gacacatcgg ttttagaaat
    48781 ggcaagatcc ttttatggta atgacaaatg gctgatggct atttgcgccg gacctacggt
    48841 tttcggcgcg gccggattgt taaaaaacat tcgatataca tcatttcccg gaaccgaagt
    48901 ttacattcag gaagggatca gggaaaaccg cgcagccgta gttgacggaa aaataattac
    48961 cggcgctggc gcgggagcgg tctttgaatt cgcttttgcg attatcgaag ctttattcgg
    49021 gaccgaaaag aaaatagaaa tgcagaaccg gactgtattc cggtcttatg agaggtaatc
    49081 agatggacaa gaaaacattt tatatcacaa cacccattta ttataccagc ggcaaattcc
    49141 atatcggaac tgcttataca acatgtcttt gcgacacgat gaaacgctac aaatccttga
    49201 tgggttatga cacccgtttt ctcaccggaa cggacgaaca cgggatgaag atcgctgaaa
    49261 gagctaagga atccggaatc actccgcaag aatatgttga caggatagcc ggaatggcaa
    49321 aagatacctg gaaacttctc gacatccata acgacgattt tattcggacg accgaaaaga
    49381 gacatgaaac gattgtcgcc gatattttcg aacagctttt ggctaaagac gacatctatc
    49441 tgggagaata ttccggtaat tactgtatgg aatgcgaggc ttatttcaca ccgacacagc
    49501 ttttggaagg cggactatgt ccggattgcg gaagaccgac aaccatcgtc aaggaagaga
    49561 catattttct gagactttcc aaataccagg acagattgtt gaaatacata gatgaacatc
    49621 ccgatttcat tttcccggaa accaggaaaa acgaggtcgt ccagtttgtc aaaaccggat
    49681 tgcaggatct ttcggtatcc agaaccgctt ttgaatgggg catcaaagtc aagagcaatc
    49741 ccaagcatgt catctatgta tggatcgacg ccctcaccaa ttacataacc gctttgggat
    49801 acggttccaa agacgattct ctttataaga aatattggga aaacggagat gaagtcgttc
    49861 atgtcgttgg caaggacatt ctgcgttttc atgccgttta ttggccgatc atgctgatgg
    49921 cgcttgattt gccgatcaag ttcagactgt tcgtgcacgg atggtacatg atgaaagacg
    49981 gcaaaatgtc gaaatcgaaa ggtaacgtca tctacccgga tcaacttacc ggacgttacg
    50041 gactcgatag tttccgctat tacatcctgc gggaacttcc ctacggtaac gacggattgt
    50101 tcaccccaga ggatttcatt tcccgcgtca atacggaact ggccaatgac tacgggaatt
    50161 tggtgtcgcg ttccgtcacg atggtcaata aatatttcga cggtttggtg agaaacacaa
    50221 aacacaaccg gtcggaatat cttggatttg aaaaggaact ggaatcgatc gccaaggaag
    50281 cggtaaatga atacagacag aatatggacg ccttccggat tcccaacgct ttggcggaag
    50341 tcaccaggct tgtcaaccgc gccaacaaac tgattgacga gacatcacct tggaatcttg
    50401 ccaaaaacga cgaggatctc ccggttcttg aatcagtcct gtaccatctt ttagaagcct
    50461 tgagagtggc ttccattctt tatattccct ttatggacaa tgcgactgaa aaagtcttcg
    50521 acatgcttgg agcggatgag gacaaacgtg atttttctac cctcatgttc ggaatccagg
    50581 aaaaatacat agtgaccaat gaaccgactc acgtgtttcc gagaatagac gctgaaaaag
    50641 aaacagaagt gatcagaacc atgatgagcg tgaagaatat cgatgaaaag aaagtcgaaa
    50701 gcaaaccgga aatcgaaatc gatctttttg acaaagtgga aatcaaagtc ggtttggtaa
    50761 aagaggctgc tattcataaa aacgcgaaga aactgctggt tttgcagata aataccggtg
    50821 acaaatcacg acaagtagta tctggaatcg ctgaatatta tgcgcctgag gagttgatcg
    50881 ggaaaaaatt gttggtcgtg accaatctca aacctgtgaa acttcggggc gaagaaagcc
    50941 aaggaatgat tctctgcggc gaaacaagtg aaggcaaact ctatgtcata gaagctaatc
    51001 ccaatttgaa tccgggagat ggagtgagat aggaatgaaa gaaaacttcc tgaaaaactt
    51061 cgacgggaaa aaagaagccc gcaccatgtt caatactgtg gtgggaacgg cgatctacgc
    51121 cctaggcatc ctgtttttca tcgaccccgc caaactatat agtggcggag taaccgggct
    51181 ttcccagctt atcgtcaacg ttgttgaatt gctttccagc aacaatatcc atatcaacct
    51241 cggtatcctg tcttttattc tgcaggtgcc gttgctagta ttcgcatatt tcaaacttaa
    51301 cggccgtttt gtgttctaca ccattgtttc ggttgttgtt tttactttga ttttactctt
    51361 cagagtcaat gaaccactga tgggatcgga tatgttgaca aacgcgctcg tgggaggaat
    51421 tctcggaggc atcggcaacg gcatgctgca ccgcgtcgga gcgtcttccg gaggaacatc
    51481 aattcccttc cagtactggt cgattaaatc gggaaaatca atgggcactt accagatatt
    51541 cctgcatggt ttgatcatat tgctttcagg agtgattttc ggattaaata tcgccatcta
    51601 tacgatgatt tcccagctca tttcttcgat tgttcttgac aagatattca ccggatacaa
    51661 tttcatgaaa cttgaagtcg tcaccactaa cggcaaagag atggctgaag ccttgaagac
    51721 cagacttccc cacggagtga cgatgattga cgctgtcgga gcctatacgt atcaagccaa
    51781 aacggtcatt tacgccgtca tatcagtaca tgagatccat aagtacgttt ccttgatcag
    51841 acagatcgat ccacaggctt tcgtggtcat gaccggagtc tccagcgtga tgggaaaatt
    51901 caccaagaag atcatcaatt gaagtcaata tttgttgact aaagtgtacc ttgatgatat
    51961 tatataaaag gtacattttt gtttgaatcc cacagctctt taaattaact tttaaggagg
    52021 aaacaaaaaa tgaaaaccta catggcaaat caacaaacga tcgaacgcaa atggttcgtg
    52081 gtcgacgccg ccggcaaagt actcggccgc ctggcgaccg aagtagcctc tgtcttgcgc
    52141 ggaaaacaca agccaacatt cacgccaaac gtggattgtg gcgattatgt catcgtcgtc
    52201 aatgctgaca agatcgaact caccggagcc aaatgggacg ataagcttta tcatacccat
    52261 tcgcactatc ccggaggact gaagacgaaa accgcaaaag tcatgatggc cgaaaagccg
    52321 acccgcatcg tcgaactggc ggtcaaagga atgttgccca aaggtaaact gggaaatcag
    52381 atgtacaaaa aattgttcgt atacgcagga cctgagcaca aacacgaagc acaaaaacct
    52441 gtcgtgatgg aactcaaagg ttaggaggtg tgcttaattg gcaaaatcag taatgtatcg
    52501 cggcacaggc agacgcaaat cagccgtcgc gagagtgata ctcagaccgg gaaaaggtga
    52561 actcatcatt aacggtcggg gattcgccga ttatcttcct tcttcgttgg cccgtctcga
    52621 cgtcatgcaa cctctagttt tgacagcgaa cgaaaacaca tttgatatct ccgtcaacgt
    52681 aaatggcgga ggactcatcg gccagtccgg tgcgatccgt ttagggatca cccgtgcttt
    52741 gctggaagta aacccggaat accgcaagat tcttaaacct gccggactga ttaccaggga
    52801 tccgcgtgcc aaagaacgta agaaatacgg tctcaaaggc gccagaagag ccccgcaatt
    52861 cagcaagcgt tagtttgaag aagccaaaaa cacaagaccc cacatcttgt gttttttttg
    52921 tcggaaacaa acccgatgat tgcaaagcaa tccgggatgc ggtagaataa acttgcttac
    52981 gcggggtgat acgatgcttt ttggcaaaca tgtgaacaaa ttctattggc ggtacggcgt
    53041 attcttcctt ttcgggatcg ccgccctttt tgtcgttgac tacactcagt tggaaattcc
    53101 gaatatcatt ggatacatca ttgaaaaact gcaaaacggg actttggacc ggattaccct
    53161 gtcaggatat atcgtttcgt taggtatcat cggtgctatc attgtcgcgg gtcgtttcat
    53221 atggcggatc ttcattttcg gaacgtcgag aagagtagac tatgatttga gaaacgaaat
    53281 gttcgcccac gcggagaaac tgagccagca atattaccag gaaaagaaat ccggcggcct
    53341 catggcgctt ttcaccaatg atttgaatgc ggttcgaatg gcattcggtc ccggtatcat
    53401 gattttcttc gatgtgcttg ccctcggcgg catggcgttt tacaaaatgt atcttttgga
    53461 tccaattctt actttggttg ctttgatacc ttctttgatg attgctgtgt tggcgacctt
    53521 cgtatctcgg gttacgggaa gaaaattccg tgatcggcaa aaagcatacg aaaatctaag
    53581 cgatttttcc caagagaatt tctatggaat tgccgtagtc aaggcgtttg ccaacgagtt
    53641 acgcgaaacc agagcctttg ccagtattaa caaggataat tatgacaaaa atctttcttt
    53701 catcagagtg tcgaccctgc tttctgtttc catcgagatg tttatcaaca tcgttcgtgt
    53761 gcttttgatt ggaattggtg gttatctcgt atacaagaca tctccgatcg atccatccca
    53821 gtcattcacc ttcaccatcg gggatttgac ccgttatctc gcttatttcg gcgccctagt
    53881 gtggccaatg atggccatag gtcggtttat caacttgcgc gcccaagcta gagcgagtct
    53941 caaacggatc ggcgattttt tggattcccc ggttgatgtc accgatgatt ttgaaactac
    54001 gccggtggaa aaaatcgacg gagcgatctc gtttaaaaat ctcactttta cttatcccgg
    54061 cgtcaaggaa ccgatcctta aaaatatcaa tcttgaaatc aaggcgggag atacaatcgg
    54121 tattatcggt cctaccggtt ccggtaaaac aaccattgcc gatcttttgt taagaatata
    54181 caatgtcggt ccgggaatga ttctgataga cgggcgtgat atcatgacgc ttccggtgaa
    54241 acaggttcgc gataccatcg gatacgttcc tcaggataat ttcatctttt cggatgcaat
    54301 cgaaaacaac atttcttttt ctcaggatac aattgaccac aaacttgtgg aacaaatggc
    54361 ccgtttcagt gacattcatg ataatatcgc cgaatttccc gatcaatatc tgacgatggt
    54421 aggcgaacgg ggcgtcacgc tttccggagg ccaaaagcaa aggatttcta ttgctagagc
    54481 acttctgaaa aaaccgaaaa tcttaataat ggacgattct ttcagcgcgg ttgacaccgg
    54541 gacagaagaa gcgatcttgg aaaacctaga taaaataaga tccaatcaga ccatgattat
    54601 tatcgctcac cgggtatcga cgatcaagaa ttccgacaag atcgttttgt tgtcggaagg
    54661 caagattcaa gcggttggta cgcacgccga attgatggag aaatcggttg aataccggaa
    54721 tatcgttttg catcaacaac tggaaagcga actggaagcg ggtgagttgg atgcctaggg
    54781 gttacggcgg cgatatcgaa agacatcgca cttattcgga caagacaatc atttcaagac
    54841 tcctggtcta tgtcaaaccg tttatcaaac aatttgtcac cgcgcttgtc atggcactca
    54901 tcgttgtcgg tctcgatctg atcggaccat tgttgatatc cgatgctctt gattcattag
    54961 gggaggaaac aattgttttt gcaaggattt tattaatcgt cggcatttat ttcgccgcta
    55021 cggcgatcgg cgcgattatt tcttataaac agacgattct actccagact actgggcaga
    55081 aagttattta cgatatcaga gcggatgtgt tccaacacat cgaatctttg tcaatcgaga
    55141 catacaacca tatgccaatc ggcaagctag tcaccagggt gacttcggat atagacactc
    55201 ttaatatgct ttataccgat gtcatcatca atctatttcg caacgcgatt acgatcatcg
    55261 gtgtcttcgt catcatgttt ttcctagatt ggaaattgac acttcttgtg ttggttgtgg
    55321 ttccttttat cgttctcgcg tcatttttgt tccggaaata tgcgagaaag gcatatcgta
    55381 acgtgagaaa cgatctcgcc aatatcaatg catttttatc cgagcatctt accaacatga
    55441 agctaatcca gattttcaat cgcgaagacc ggaaattctc tgagtttaaa aggcaaaacc
    55501 aaaaattgaa ccgcagttat cttcaacaaa cattcacgtt cggcatctac cgcccgctca
    55561 tgtatttgct ttatgttgta gctttgatga taatcttgtg gcaaggttcg cttgacacga
    55621 tgcttcagat catcactttc ggcgtgcttt acgccttcac acaatacata aacaaattct
    55681 tcaatccgat ccaggatctg gcagaaaaat acgatactct ccaagccgct ttcacagcgg
    55741 gagaacggat cttcgagatc atggacacat cccccttggt gaaagacgac cttgatgcga
    55801 tcgatatcga gagtttgaaa ggaaagatcg aattccgaca tgtctggttt gcctatgaca
    55861 acgaggattg gattctgaaa gacgtgtcgt ttatcgtaaa acccggggaa actctggcgt
    55921 tagtcggagc taccggttcc ggcaagacga cgatcatcaa tctgatagtg cgaaactatg
    55981 acattcaaaa aggcgagatt ttgattgacg acatcgacat taggaaaatc cgtctggcat
    56041 ctttacgaag caagatcgga cagatgccgc aagatgtctt cctgttttcc gggacggtcg
    56101 aatcgaatat cagaatgcgt gacgaatcga tagagatgga taaggtcgtc caatcggcaa
    56161 aatatgtcaa cgccagccat ttcattgaaa aattgccggg aacgtatttg gaaccagtaa
    56221 gggaaagagg caacaatttc tccgctggcg aaaggcagtt gctttcgttt gccaggaccg
    56281 tagcccatga tccgtcggtc atgatcttgg atgaagccac cgccaatatc gatactgaga
    56341 ccgaggcttt gatccaggag tcacttggga aaatgatgaa catcggcacg atgattgttg
    56401 tcgcccaccg gctttccacc atccaacatg ccgactgcat catcgtcatg caaaaaggaa
    56461 agatcgtcga atcaggaaat caccacgatc ttctacgtaa aaaaggctat tatttcggac
    56521 tatatcaact acaatacgaa cgtcccgaat aatgaagaaa taccgattga ttatgttata
    56581 atataaacgt aatcgcaaaa gccctgaata atggggggtg atgtaagatg aaaagaatct
    56641 ttctgcttgt ggcgcttctg attgttgtca tgttcatata cacattctcg ttggctcagt
    56701 acataagcat gtcttttttg cttgacattt tgcccaataa tattttcata tacatcataa
    56761 tcgggttttt atggttgttg gcgtttttca tcgccatcag gataatcatg aaacagacat
    56821 acgcttacaa aaaaagttat tggatactga taattttgtt gaacccgatc cttggtatca
    56881 ttctttatgc catattcgcc cgcgattttg tggccgatag attccacaga acaaggccgt
    56941 taatcgctaa caaagctttt ttgggacttg aggaagcgac ccatgtcgat tacgataaac
    57001 gcgaatacgg gaatattttt agatttatcc gggaaaatac cggtagatcg gtatatcaag
    57061 atgacaccta tgtcgaaatt ctgaataacg gcgacatatt tttccccagg ttaaaagatg
    57121 cccttgaaaa agcaagaaat tatattttaa tggaatttta tataatcaaa accgacgaaa
    57181 tcggatgtca aatccttgat atcctcaaat ccaaggcaaa acaggggctg gatgtatatt
    57241 tgatctatga tcatttcggc agcaaccgtc atttagataa aaaatacatg aaagacctgg
    57301 aatcatccgg agtccatatc ggggttttcg atccccaaac gttgtccgtt tttaacagca
    57361 atctaaattt tcggaatcac cgaaaggcga ccgtcatcga cgggaagatc ggattcgtcg
    57421 gaggcatgaa tttagcggac gaatacaacc ataaatcccc gaaattcgga ttttggcgcg
    57481 acacacatgt cctgatggcc ggcaacgggg tgaccagcgt tcagaatgtt tttgtcaagg
    57541 attggtatta tatcaccaaa gaagtgctgg acatgccgat ggacaaaacc gacgccaatt
    57601 accgcgggct tttctccgta atagaatcag gaccggattt cgaggacgga cagatccgcg
    57661 acgtctatct caaaatgatg tttgccgcaa aaaaaacgat cgatatcgtg acgccctatc
    57721 tcatcatcga accggaaata atggccgcca tccacgtcgc tgtcaaaagc ggagtaaaaa
    57781 tacgtctact tgttccggga ctgtcagatt atgtgatggt cggcttggcc acgaaatcct
    57841 attatgagca gttgctcagg acgggtgtcg agatatatga gtataaggat catttcgtgc
    57901 attcgaagat tctgatcgta gacgataaga ttgcatcggt gggatcggtc aattttgatc
    57961 cgcgcagttt ccatctcaat ttcgaagtga cggcggtttt cactgacgaa gccgtcggtg
    58021 tgctttccga aagtttccgc gaagacctgg aaaaatcaaa tcgaataacc atggaagaat
    58081 gggctcgacg gggagcgatg aaacgtttca ttcaagctct gttaaatctt tttagcccca
    58141 tattctgaca attcttgatt aggaagtgat agtatgtcgt taccagaaat tcttggattg
    58201 atttccgtaa tattactgac gataattttg gttggaatcg catatcttgt tttccggcgc
    58261 catccaaaag acgacggctt tgccggcatg gacaggttgg aaactgattt atccaaacag
    58321 gtcggtgatc tcaaagatac atttatgaaa acaatgttcg aatccatgcg gaccttttcc
    58381 tccgatgtca acaagcaact gttggaaacc agcacgaaaa ccaccggcaa catggcagat
    58441 ttccaagtca aggtcaacca ggagttggct tcttttcagg aaaagatcaa caccaagctg
    58501 accggcgatt tcaccggact gaccaattcc attgatcaaa gaatgaacgc gatcaatgcc
    58561 aaggtcgagg agaggctcag ccaaggtttc aaggatacca aggaaacctt tgtccagatt
    58621 gccgaaagag tgaaagtaat tgatgacgcc caaaagaaga tcgaggagct ttccggcgag
    58681 atgatcggac tccagaacat cctgaccaac aaccaggccc gcggatcatt cggagaatac
    58741 cagctcaacc agttgctttt cagcgtgttc ggcgaaaacg ataagttata tcagactcaa
    58801 tatacgataa aagaacagaa aggcaaaaag gaagcggttc gcgccgacgc cgtgatcttt
    58861 ctgcctaaac cgaacaatct ggtggcgatc gattccaaat ttccgttttc ttcctattcc
    58921 aagttgttcg acaatgatga attgacaaag gaagatgagg acaaactcat tcagagtttc
    58981 ggtcacgagg taaggaaaca catcaccgac attgccgaca aatacattgt tccaggcgtg
    59041 acagccgaat atgccctgat gttcgtcgcc agcgatggca tcctggcttt gctgcattca
    59101 agactcacca atgttatcga ttatgctcgc tccaaagcgg tgacgatagt atctccgaca
    59161 acactcatac ctttgctttc gagctactgg gccatggtca ttgattacga acaaagccgg
    59221 tatgccgccg agatcaaaaa acaactggga cttttgagtg gcgaattcca aaaatttggt
    59281 tcggactggg agaaactcaa ccgtggaatc gaccaaattt caagacaatc gcgtagcgtc
    59341 aacgaccatg ttgaaaagat cacgtccaag tttgacaaga tccaaatggt cgacttgccg
    59401 gaaaaaccgg ccattcaaga tgccacagat gacgaaaatg acaatgaagc gcaaaaagac
    59461 gcggtgccag atgaattaaa ataaattaga cttgcatttc aataacgaaa aatgtataat
    59521 attcatagca tcagaaaaat cgaaggagaa acaaatgaac ggatcagtaa aatggtttaa
    59581 cgcagaaaaa ggttatggat tcatcactga tgaaaatggc aaggacatct tcgcccatta
    59641 ttcagcaatc caaggcgagg gctacaaatc gctcgaaaac ggtcaagctg tcagcttcga
    59701 cgtcgtcgag ggagccagag gcccgcaagc cgcgaatata gtgaaattat aatttgactg
    59761 tatgttaaac aaaaagagga cgccgacagg cgcccttttt ttctttcctt tggtttcatc
    59821 ttgctaaact aaccccgata acctataatg ttatatacgg aaaggatgtg gaattatgca
    59881 gattttcaat acttatacca accaattgga agaattcgtc cctttgaaaa aaggcgaggt
    59941 gtccatttat gtctgcggtc cgaccgtata caactatatc catatcggca atgcccgtcc
    60001 ggttgtcgtt tttgatgttg tgcgaagata cttcgaatat aagggataca aggttaaatt
    60061 cgtatcgaat tttacggatg tcgacgataa aataatcgcc aaagcaaaac aggaaggaaa
    60121 aacagaaaag gaaataagcg aaagatacat ttcccaatgc ctttccgatg tccaagccct
    60181 cggttcggaa accgactata tcaagccaaa ggttaccgaa tatatgccgc acataattga
    60241 ttatatcgac gaactggtaa aacttggcaa cgcctaccag aaagacggcg atgtctattt
    60301 ccgtgtttct agtgttcccg attacgggaa actgagcaat cgcaggataa acgatcttat
    60361 cagcggcgct agagtcgaag tgaacgatct taaggacagc ccgcttgatt tcactttatg
    60421 gaaaaaaacc gacaccggcg tgaaatggga ttcccctttt tccaccggac gtccgggatg
    60481 gcataccgaa tgcgtcgcga tgatcgatga catctttcat ggtatgatcg atatccatgg
    60541 cggtggaacc gacctcatat tcccgcacca cgaaaacgaa atcgcccagg cagaagccat
    60601 gcatcacaac catatcgcca gatattggat gcataacgga cgcttgaatt tggaaggtga
    60661 aaaagaaaaa atgagcaaaa gccagggaag cgtcgtgctt gtcaaggata tctacgacaa
    60721 ggcaatgccg tttcgcttgc ttttgttgtc gacgcaatat cgcactccgc tcaattattc
    60781 cgacgataac ctgaacatgt attcggcgga atgggataaa attgtcgcca caatcaactc
    60841 tgtgcatcga accctggctt tcgacaatta tttttcgcaa caaaacgata acgtgaagtc
    60901 aaacgaagtg tcggccctaa tgcaggcgtt cgaagatgcg atggatcagg atttcaacac
    60961 accaaacgca attacttcct tgcaggcact tgtcaaaatg gccaacgtcg aactgcgcaa
    61021 gaaaaataat gatcccttgc tggcggatat tgaaaagacg cttatgaaaa tgctcggtat
    61081 cctgggactt gatcccgaaa tccgggacat aaccgaagaa gataaaacat tgtatctgca
    61141 atggcagaaa ctccgtcagg atggaaatta tgccgaagct gataaaatca gggacattct
    61201 tcaaaaaagg aaacttctat gaatcttcag aacggattgt cgctagccta catcggagat
    61261 gctgtctatg aactggagat cagaaaacgg cttctcgaca aagggctgac aaaggtcggt
    61321 gatcttcaca aacaagccat ccgattcact tcggccggcg gccaaagcaa ggcgatggat
    61381 ttgttagaag caacactttc cgatgaggag ctttctgttt tccgccgggg acgaaacacg
    61441 gaaggtggtc gcaaaccgaa aaattccgat ctcggtacat atcatcgggc caccggattc
    61501 gaagctctga tcggatattt atatctcgag aaaaaagaag acagactaac ggaactgatt
    61561 aatgcctgtt ttgcgggagt ttccgaaaat cccgaatgaa agcaggcctt tttcttcttt
    61621 gcgaaccaac cttaaatcaa acagaaaaaa cggaaaatat gttaaaatta aagtggtgaa
    61681 aatatgagta tatacattta cggaaaaaat ccagtccaag aaatactgaa ttccggaaaa
    61741 cagatcatcc aggcttttgt catggaaaaa accaacggcg atcttttgac gcaatttcaa
    61801 aacagggata ttccgacgac tttcatggat cgcaagaatt tcgattccaa atttcccggc
    61861 gtgaatcagg gagtggctgt cgaaatcaaa aattacgaaa cggttacgat agaggagttt
    61921 ctgaaaaaga ttgatccgaa agataatccg cttgttttga tgttggacgg catccaagat
    61981 ccgcataacc tcggagccat agctagaagc gccgaagctg gcggagtcaa gggaattatc
    62041 attccgcaag atcggagcgt cggtataaac gccactgtcg tgaaggcttc agccggagcg
    62101 atagaataca tggatataat aactgtaacc aatctggtga acacagcgga aaagctgaaa
    62161 gaggccggat tctggatcat cggaacggcc gccgatgcga ctgtcagtta tacggaggcc
    62221 tatgtcgaca gaccggtttg tttggttatc ggcagcgaag gaaaaggtat gcaccggctt
    62281 ttgcgcgaac attctgattt gcttgtggca atcccgatgg ttggcaaaat aaacagcctg
    62341 aatgcttcgg tcggggcagc gatcctgata ttcgatattc tcagaagaaa gaagggttga
    62401 aatttgcttt ttcgcaatta taatgatttc gagatcatca gcctgatcaa acaaggaaac
    62461 gaagaagcgc tggaactgat gattgacaaa taccgtttcc tgattgcgaa aaagattgga
    62521 aaattcaacc tggccgacga atacgacgat gcttttcagg aaggcgtaat cgtcctttat
    62581 cgttccgtta tgagattcga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    62641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    62701 ngaccgcatc cggcataagt taaaaatgca tttagaatta tgattttctt gacaaaacag
    62761 ccattatttg atacagttat taaagcaagc gagttgatca aagatgaaag acaaagcggt
    62821 cctggtttgc caggaatgcc actcccgcaa ttatgaaata aaaaaaagcg acaacaggac
    62881 aacacgactc gaagtgacga aatattgcaa taaatgcaaa aaacacactt tgcacaagga
    62941 aagtaagtag gaggaatacg catcatggct gaagatgtaa caaagaagcc ggtcgccggc
    63001 gaagaaaaag ccaataaaaa accattgaaa gtcaagaaaa gcaaaatgga gatggaagaa
    63061 atccattccc gcgtaatgga tattcttaaa aaagaataca agttcgagaa ctggttgctg
    63121 ggaatcctgt cgccaattct gattttgtat ggggtttaca tcgtcatggg cacattcgga
    63181 agtttcgatc tcgccggagc gctcggtgat tctgaaatcc cgttttttgc tttctttttc
    63241 gatacgacgc tgaaaagagt gttgaccggt tctttcctga tcttgatcgg tactttggtc
    63301 atcatctatt tggtaatccc attcctgcgt cccagttttg tggaaatgaa aaaagtatca
    63361 tggcctacaa taaaaacatt ggccagaaat actgcccgcg tttttctttt cttaatcttc
    63421 ctttctgttg tattcgcttt gttcggattc gcgttggatc cgttattcaa atggctgatc
    63481 agcctataat aagggcttgg tgctaagtga tggatagcag taaaagaatg tggtatatcg
    63541 tccagactta ttctggattc gagaattccg tcagggataa tctgcaaaga agaattgaaa
    63601 ccatgcagat ggaagatctc atcttccagg tcatgatccc cgaagagctc agaaccgaga
    63661 aaaaagcgga cggttcgata aaagaaaaaa tagtgaagat gtttccggga tatgtcttca
    63721 tcgaaatgat cgtaaccgac gattcatggt ttgtggtcag aaacacaccc ggagtaaccg
    63781 gatttctggg cagtagcggc ggcggcacca agccggtccc tctccttcct gatgagataa
    63841 atccaattct caagaaatgc ggtttgttgc aggctcaaaa gttgaacgcg caaccgggac
    63901 aaaacgtcaa agtggctacc ggaccgtttg ctggacaaat cggcgtcatc gattcgatcg
    63961 acgacgaaaa aggcaaagtc aatgtgcttg tggacatgtt cggaagaaaa acgccaatcc
    64021 tattggattt cgcggacatc gaaataattt gaatagtcgg caccatctgt gccggctatt
    64081 tttttcacca gcatatacac atgttgcatt gttatatata atatagtatg aaaaatgaat
    64141 aagcttcctc aaatatgtga cagaaaggtg ctcccgatgt taaaacttgt aaatgtatcg
    64201 aagtattata actccaacaa cgttgtcgct ctcggcctta gaaaaatcaa tctggaatta
    64261 ggcgtaaacg aatttgttgc cgttgtcggt gaatcgggct ccggaaaaac aacgcttttg
    64321 aacgttattt ccgggatgga ctcatatgaa gacggcgaaa tgtatatcaa cggtcaggaa
    64381 acatcctatt tttccatcaa cgatttggaa aattaccgca aaaaatatat tgccttcgtt
    64441 ttccagaatt tcaatctgat tgattcctac accgtgttgc agaatgtgga actccctctg
    64501 ctattgtcgg ggaagacccg caaagaagcc agagccatgg caatggaaat catcaagaaa
    64561 gtcggactgg agaagcatgc ccgtcacaaa gccacaagat tgtccggcgg tcagaaacaa
    64621 agagccgtca tagcccgcgc ccttgccaag gattgcccga ttatcgccgc cgacgaaccg
    64681 accgggaacc tcgattccga atcggctgcc caaatcatga aactgcttca tgaaatagcg
    64741 aaagacaaac tggtaattgt ggtcacccac gatttcgagc aagtgaagga atacgccacc
    64801 cgtaaaataa gaatttatga cggggaaatc gtcgaagacg tccaggtcca tcattccaag
    64861 ccggccgatc ttccggaaat cgaggaaaat ctcaagcgtc ttaaaccgtg ggaacaggtc
    64921 aagatggctt tcgtcaacct gatggcggtt ccgaagaaaa cgattctgat ggtatttgtt
    64981 ttctttttcc tttcttcgtt tgtggcgctt gtttacggag cgtaccgcat cagtgtttcg
    65041 tatgttgacc aatattatta ctatacgagc gacgatgagt ttttctccaa cacctcgatt
    65101 tcccgggtca tactgaacaa aaaggattat tcaccgttta ccgcttcgga attggccgat
    65161 ttatccgaca tacccaatgt ccagacggtt atctataacg actacttaat cgatgtatcg
    65221 atttatatga tttcgaacga acaggtagcg aacaacggca ggagccggaa tatgctcgcc
    65281 gatgttctga tcttgccaat gtcagtcata aaagacgcga atgtgttaag ctatggagaa
    65341 ctccccgacg aagaaggtga ggtcttgttg gcgctacctg cggcggaatt ggaaaagagc
    65401 gacctgtatc tgaataatgc ctacacaatg aatggctttt ccaatatcta tctttatgat
    65461 acagctgaat atttgataac cggactggtt gatgccggcg atcttttcat ggatgaaacc
    65521 aatgactata taattgtgtc cgacgccgaa ttcgcccgcc tggtcaaagt ttgttacctg
    65581 gcgttcacaa aaacgttgag tctggatgtt ttggattccg aaggcgatga tttgggcttg
    65641 ggcgaatggt tctcatccat gttcatgtat aacggcctgc aaatcctggt agacgattcc
    65701 ttgacagaca gtgaaatcgg attcgacaca gccttatctg attatctatg ttacgatcct
    65761 tacggaacag gtgatcattg cggaatcgaa tttgccgatt tgacggtgga ggacgcgtac
    65821 cacgatttga gcctctcgga tttggaagtg actttcatag acaatcagtg gatcgccatt
    65881 tatctaagtc aaaacaacta cgacagaatt ttttattccg acatatacca ggtatcgctt
    65941 ttgaccaaat cggatatcaa tgtcggacgt ttggtcagtg atttggttaa gataaaagac
    66001 ggaacggaag caaagtacaa tgttgtttat ccctacgaat ttgaaaccga cgatcaattc
    66061 ggctctgcca tgtcgttctt ccagcttttg gggatgttga tgacggtctt ggcaacggct
    66121 ttggcgtcga cgcttttgag ctatattatt ttccgggccg taatcaatac gaaattgaag
    66181 gattatacga ttttccggac agttggcgcc acccagggag tcatcagact gatgatttat
    66241 ctggaagacc ttttcgtggc ccttttcagt tttttgcttt ttgtgatcat gacggtcagt
    66301 ttcaaattgt ccgacgctat tacaagatac agcgcttttt acggactaaa ggccttccga
    66361 ttccaggatt atgttctgtt ctttgtgatt ctgattttga tgtcaatatt catatccggc
    66421 cgttattgca accgtatttt caaagatacg gttacgacga ccatgaagag ggggtaaggc
    66481 acgatgataa agataattga tctcaacaaa tatttcaaca agggcagatc caatgagatt
    66541 catgtcatcg accatacctc gcttgaattt ccggaaaccg ggttgatagc gattaccggg
    66601 ccgtccgggt gcggaaaaac caccctgctc aatgtgattg gcggactgga ccgcttccat
    66661 agcggagaaa tcgactttga tgaaacgaaa atcatgaagt acaagtcttt gcaatgggat
    66721 ttggtcagaa acaaatatat cggatacatt ttccaaaatt acaatttgct gccagataaa
    66781 acggttttcg agaatgtgga aatcagtctt aacatggctg ggttgtatga taaagaaaaa
    66841 gtagaagaaa gaatcaatta tgttttggaa agcgtcggca tgtacaatta cagacgccgc
    66901 aacgtgcaag ctctttccgg cggtcagcaa caaagggtag cgatagccag agccatcgcc
    66961 aaggacccga aagtggtcct ggccgacgaa ccaaccggca atcttgacgt gaacaacacg
    67021 tttgaaataa tgagcatcat caaaaagatc agccaaacct gtttggtcat tttggtgagt
    67081 catgaacgcg agattgtcga tttctacgcg gacagagtca tagagatcaa ggacggcaaa
    67141 gtcgtcaatg actatgaaaa cgccgggaac aagacgttgg aacatgtcga ttcgcgaaac
    67201 gtatatctac ttgatctgga aaaggaagaa a
//
LOCUS       DAOPJL010000003        57961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_555_length_57961_cov_11.666028, whole genome shotgun sequence.
ACCESSION   DAOPJL010000003 DAOPJL010000000
VERSION     DAOPJL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 57961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57961
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_555_length_57961_cov_11.666028"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1058)
                     /locus_tag="PLP02_00870"
     CDS             complement(<1..>1058)
                     /locus_tag="PLP02_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPF42051.1"
                     /translation="YLETTSRMIRDWIETMMNDIPCEVCGGKKLNPAALSVKVGGLNI
                     DDFSSLSILHLLDFIETLELTDREKEIARPILKEIKERLRFLMNVGLDYLTLSRSSGT
                     LSGGEAQRIRLATQIGSRLTGVLYVLDEPSIGLHQRDNLRLIDTLKGMRDLGNTMIVV
                     EHDMEMIENADFVVDMGPGAGEHGGKVIFAGTPKDIVKDENSITGKYLTGKMSIPLPK
                     ERRKAERGYLKIIGATQNNLKNIDVDIPIGNLTVVTGVSGSGKSSLVNEVLYKNLVNL
                     ITRRNENAGTCKGIGNYEAFDRIINIDQSAIGRTPRSNPATYTGVFDEIREMFAKTKE
                     AKIRGYDKGRFSFNVKGGR"
     assembly_gap    1059..1158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1159..2048)
                     /locus_tag="PLP02_00875"
     CDS             complement(<1159..2048)
                     /locus_tag="PLP02_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPF42052.1"
                     /translation="MNDKIIIKGARENNLQSVDLVLPKNQLIVMTGLSGSGKTSLAFD
                     TIYEEGRRRYVESLSAYARQFLGNMEKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTV
                     TEIYDYVRLLYARIGVPYCPEHGIPITSQTIQEMTNRLLEKENAKLTIMSPVVRGKKG
                     MHEKTLEELRRTGYNRIRINGEFHDLEEDMDLEKNKRSDIDVVIDRLVVKPGVRSRLY
                     DSLETAAKLSDGLVKVLANEDELIFSEHFSCPICGFSIGKLEPRLFSFNAPYGACPEC
                     SGLGFKRKIDPNLLIPDTGKS"
     gene            complement(2136..>2525)
                     /gene="ligA"
                     /locus_tag="PLP02_00880"
     CDS             complement(2136..>2525)
                     /gene="ligA"
                     /locus_tag="PLP02_00880"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932384.1"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPF42053.1"
                     /translation="KEDLLELSDIGEAIASSLTEYFQQSKNAAMIHELEDLGLNMVAS
                     KKFTSTGSSFSGKTIVLTGALEGYSRSEAKDRIESLGGNVSSSVSHNTDLIIAGTDAG
                     SKLTKGKELGIRIIDEQEFNRLLEEES"
     assembly_gap    2526..2625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2626..3950)
                     /gene="ligA"
                     /locus_tag="PLP02_00885"
     CDS             complement(<2626..3950)
                     /gene="ligA"
                     /locus_tag="PLP02_00885"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPF42054.1"
                     /translation="MTVMERLKFLRDTLNKYNYEYYVMDNPSVPDSEYDTLMNELLRL
                     EEEHPELVTKDSPSQRVGGGIQDRFVKVIHERPMLSLADVFSKEELIDFDQRIAKETK
                     DYSYTAELKIDGLSVSLQYDNGYLVRAATRGDGVTGEDITENVKTINSVPLKIPYLAP
                     VEVRGEIFMPTKSFNRLNEQKLANNEEPFRNPRNAAAGSVRQLNPQIVRKRNLDVFVY
                     YVMDRKIVSDHYKALQLAKDWGFRVNPLNHRCKTIDEVISYIDEMADKRNELPYDIDG
                     IVIKVNEYNLYDRIGYTAKYPKWAIAYKFPAEEVVTVLKDIRFQVGRTGVVKPVAELE
                     PVMISGSLVSRATLHNEDFVKARNIRIGDHVIVRKAGEIIPEILGSIPEKRDGREKPF
                     IMAETCPICKSRLSRKENEVDIYCMNPDCPGKSIEGLIHFASRDAYNIDG"
     gene            complement(3956..>5695)
                     /locus_tag="PLP02_00890"
     CDS             complement(3956..>5695)
                     /locus_tag="PLP02_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPF42055.1"
                     /translation="NPEQLEFLPEPIKSVMEVIYPAYQKYLKKNNLVDFQDLLVLILE
                     LFTKFPEVLEKYQNRFQYILVDEFQDTNNLQYEIVYNLAKTHHNLFIVGDEDQSIYSF
                     RGSNIENIRKFMKDFPEYHKVILNQNYRSKTNILEAANSVIDNNHNRIPKQLFSELGE
                     GEKVQHFKAETDEEEAYYIYNKIKTLARTGYNYRDFAVLYRNNAMSRRFEDMFLRYGI
                     PYKVIGNISFYKRKEIKDVIAYLRLLIDMGDNYAFERIYNEPKRGVGPTSFDKIKEYA
                     TKENVKLTEAIDDDIPGIGIKGLIRLRELKANLQEIRRNIEEINLLDTYDRLLEKSGY
                     LEMLQNEDLAADRKIEAERRIDNLNEFKTIILEKIKEFDRDVTNYEKLLEILTDLALR
                     EEAEDKLTDDEYVSLMTVHSAKGLEFKVVFVACLEQALFPSGQSLQERANVEEERRLF
                     YVALTRAKEMVFLTNARERFIYGHLASNPDSQFIGEIGKEYIEHSGLSKPREIKLERI
                     QSQRKHVLPAVESKEYESVDNKLSIGDKIEHKTFGIGVVVALSDNRATIAFGHETGIK
                     ILMSDHPSIKKVR"
     assembly_gap    5696..5795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6033..6257)
                     /locus_tag="PLP02_00895"
     CDS             complement(6033..6257)
                     /locus_tag="PLP02_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42056.1"
                     /translation="MKNKIVYLLLYLIFAIIGWFGTIINLIMAIKGDSSGTVITFTIV
                     FALMAILVTAAFIKRLIRLLIDIKINKENK"
     gene            complement(6257..>7447)
                     /gene="glnS"
                     /locus_tag="PLP02_00900"
     CDS             complement(6257..>7447)
                     /gene="glnS"
                     /locus_tag="PLP02_00900"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HPF42057.1"
                     /translation="NLRLFEEMRQGKYQDGEKTLRAKIDMASPNINMRDPVIYRILHA
                     THHNTGDKWCIYPMYDFAHPLQDAIEGVTHSLCSLEYVDHRVLYDWFIDNCETPHKPH
                     QYEFGRLNITNTIMSKRYLRQLVEEKYVNGYDDPRMPTLAGLRRRGFTKDAIVSFILS
                     TGLSRINSTVSSEMLENTLRADLVDKTKRMHAVLDPLKVTITNYPNNETEMLDVTNNV
                     DNEGLGSRKLPFGKTIYIEKSDFVPEKPNKHWKRLSVGVEVRLLQAYFIKCEDIVYDE
                     KGEIKELRCTYDEKTKSNSGFNERKPNGNIHYVEATTAIPATFNLFEPLLIDSDGDRP
                     LKERLNPTSWQTKKGFVEPDLKNSVPGEKFQFIRNGYYAADYESKSDNLVFNRIVELK
                     SSFR"
     assembly_gap    7448..7547
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7548..8046)
                     /locus_tag="PLP02_00905"
     CDS             complement(<7548..8046)
                     /locus_tag="PLP02_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009335926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPF42058.1"
                     /translation="MEIESNFIKTIMEEDLQSGKIKEIITRFPPEPNAYLHIGHARAI
                     VTNFELARQFGGYTNLRFDDTNPINEDASFVEAIKEDIRWLGYEPANIFFGSDYFEKT
                     YEYAIKLIKKGLAYVCDLSQEEMSAYRGTLTEPGKESPNRNRSVEENLRLFEEMRQGK
                     YQDGEK"
     gene            complement(8050..9054)
                     /gene="tsaD"
                     /locus_tag="PLP02_00910"
     CDS             complement(8050..9054)
                     /gene="tsaD"
                     /locus_tag="PLP02_00910"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002482771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPF42059.1"
                     /translation="MIVMGVESSCDETSVSLVENGKTVLSNEVLSQIDIHQAYGGVVP
                     EIASREHVKGITMVFDQAMQKAGKSYKDIDLVAVTNGPGLIGSLLIGINAAKVVSLNY
                     DIPLVGIQHIAGHIYANGIEHEMEFPLLALVVSGGHTELVLMKEHYEFEKLGETMDDA
                     VGEAYDKVARVLGLPYPGGPQVDKLAATGKDIYGFPRPMIDSGDFNFSFSGLKSHVIN
                     LHHNLEQRKEPYEVADFCRSFQEAVTDVLVKKTKEAANQHNVKQVIVAGGVAANRGLR
                     AKITQEITGVPVFFPAIRYCTDNAAMIAVAGYFKYRKTGKADSYMLNGYSRLDLVEEE
                     "
     gene            complement(9051..9515)
                     /gene="rimI"
                     /locus_tag="PLP02_00915"
     CDS             complement(9051..9515)
                     /gene="rimI"
                     /locus_tag="PLP02_00915"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPF42060.1"
                     /translation="MKGFAISPMTHGDLPEIIKADRLVLGHTLGEQTLANELDINPFA
                     HYFVMREETTGKLAGHVSIWIDLKNAQILNLYVLPEFQHHGLGDDIMRFCIDYLSEYQ
                     VENITLEVRPSNFRARNLYGKFGFRQVAIRRNYYDNGEDALLYLKKCEVKVQ"
     gene            complement(9512..10120)
                     /gene="tsaB"
                     /locus_tag="PLP02_00920"
     CDS             complement(9512..10120)
                     /gene="tsaB"
                     /locus_tag="PLP02_00920"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPF42061.1"
                     /translation="MPKSWKRLAIDTATDYVYLSLLENNREKENIIKSGNHDHSVTTI
                     PLMAELLERNGWKLNELDEIIVGIGPGSYTGVRIGVTIAKMIGYLNHIKVSAVSSLAL
                     LASGSEAKIIVPLIDARRQNAFIAAFKQNNGKLEYMKPDCLANKEEFLDGMKPMDYEV
                     VDSGKYNIEKIITSGLLISIENIHELVPNYLQMTEAERNIQQ"
     gene            complement(10077..10547)
                     /gene="tsaE"
                     /locus_tag="PLP02_00925"
     CDS             complement(10077..10547)
                     /gene="tsaE"
                     /locus_tag="PLP02_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPF42062.1"
                     /translation="MAAIKLQIIVLSLADMDKLAKVISGTLHPGYVIGLSGDLGSGKT
                     TFTKYLAKHMGITSNVNSPTFTLLKTYSGNLPLYHMDVYRLEQGGDGYELEEFIYGDG
                     VAVIEWYQFANGILPKEFLKMDIVISGESSREITIEGCGAYAKVVETIGDRYRN"
     gene            10741..11616
                     /locus_tag="PLP02_00930"
     CDS             10741..11616
                     /locus_tag="PLP02_00930"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPF42063.1"
                     /translation="MFLIIHNPLSSNMKSKIKTFMMVRFFKKNHIPFNLRSTLKIDNL
                     NEYLDNNPSITDVLFLGGDGSINHLINNVDVTKIKQTIYLGKSGSGNDFLRSLKKIGS
                     GNITIGEAVTDKGSTKFINGVGAGIDATVCYYVNRDKKKKRMSYFKNVFSAMKAFSPS
                     AVDISIDGKEYHYDKCYFAAIQNGEYFGGGMRVAPNADILSDEYRICVAHSMSKSMLQ
                     FLLMTIYSGLHVHFKKWVTLFTGKDITVKFADKRYFQAEGEVMVDVSEFSVKKNMSKE
                     FHAFDKRKIKKSLVK"
     gene            complement(11678..12982)
                     /locus_tag="PLP02_00935"
     CDS             complement(11678..12982)
                     /locus_tag="PLP02_00935"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPF42064.1"
                     /translation="MEKILELDRSYLSAFVSDTEIKSLQKEITAARKTLEQKTGAGND
                     FLGWLDLPVNQDKEEIARIQLVAKKIRKMAKILVVIGIGGSYLGTRAALDMLKPYFEN
                     RKRLEIIFAGQNLSSEYISDLLIYLKDRNFAVNVISKSGTTTETAIAFRALKELLETK
                     YGIDGAKERIVVTTDKEKGALRKMASKEGYESFVVPSNIGGRYSVLTPVGLFPLACAG
                     IKIAEIMKGAKAAKKEYKKADVFANDVHLYVAIRNWLYRSGKSVEMLVSYEPKLASLA
                     EWWKQLFGESEGKNGEGLFVASANFTTDLHSLGQYIQEGRKLLFETVLLVEEPNLDHV
                     IKEDKENLDQLNYLAGKTVDYVNKKAAEGTLMAHSSGEVPCIVAKIKNLSSFTFGYLV
                     YFFELSCAISGYVLGVNPFNQPGVEAYKKNMFNLLGKPGNIK"
     gene            complement(13067..13399)
                     /locus_tag="PLP02_00940"
     CDS             complement(13067..13399)
                     /locus_tag="PLP02_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HPF42065.1"
                     /translation="MKEGDIVRGKVTGIKPYGAFVKIDDQYDGLVHISEITDGFVRNI
                     EDFLKTGDEIEVQVLKINEGNKLSLSYKSTRKHKRGKYVDIQLSTGFKPFEKTLPEWI
                     AKYQKGGE"
     gene            complement(13495..13989)
                     /locus_tag="PLP02_00945"
     CDS             complement(13495..13989)
                     /locus_tag="PLP02_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012864928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPF42066.1"
                     /translation="MEPDSKTIHDHCISLLQERGVKISDITDIVYDLQAPYIENLNRQ
                     VCEDAVYRVLAKREVQNAVLTGVELDKLAEQGKLSEPLESLVNDDNPLFGIDEILVLS
                     ICNLYGSIGLTNFGYVDKTKPGIIGKLDREGKETDKCHTFLDDIIGAIAAAAASSVAH
                     NNAK"
     gene            complement(14007..14243)
                     /locus_tag="PLP02_00950"
     CDS             complement(14007..14243)
                     /locus_tag="PLP02_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPF42067.1"
                     /translation="MTYEEIIAEVQEVINVIRPYINRDGGDIEYIKFEDGIVYVRLTG
                     ACVGCAGFEDTLKELVEDTLLERVPGVIGVEATV"
     gene            complement(14265..14549)
                     /locus_tag="PLP02_00955"
     CDS             complement(14265..14549)
                     /locus_tag="PLP02_00955"
                     /inference="COORDINATES: protein motif:HMM:NF018022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HPF42068.1"
                     /translation="MIETEHGNFEMIKNHRDAFIMDDFNKRYTDAFDIYPYVVGDYSA
                     GILRLKGFALEEKTNSYRTIPDYLAESCPMNCAYFILKNPNYDPKKKRDE"
     gene            complement(14638..15129)
                     /gene="ispF"
                     /locus_tag="PLP02_00960"
     CDS             complement(14638..15129)
                     /gene="ispF"
                     /locus_tag="PLP02_00960"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008802141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPF42069.1"
                     /translation="MIRIGFSKDIHPLEEGKPFILGGIKLDYHKGAVGHSDADCLMHA
                     IAESLLGALALGDLGKFFPSDNPVYKGYDSGLILKEVYDYIKKKGYEVINLDCMISLE
                     NPKLRPLIDDMRANIARIMEVQVERISIKATTYEGIGPVGEEKAIICEAVVLLENEVF
                     MML"
     gene            complement(15126..15776)
                     /gene="ispD"
                     /locus_tag="PLP02_00965"
     CDS             complement(15126..15776)
                     /gene="ispD"
                     /locus_tag="PLP02_00965"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPF42070.1"
                     /translation="MYTALIMAAGQSVRSGLGHNKNLHPLKGKPMILYSVERFLGDPE
                     CSEIIVVVNNDEKEEFLNVLPKKIRVAVGGDTRQESVLAGLRLVKEDVVMIHDGARPN
                     LSRQALERLKEGLRFHSAAALAISASDTLKLVRDNFVFGDIDRESVKRLQTPQAFPTD
                     RIMDAHRKARDNNRTYTDDVSVYQNETGLPIWLVEGELKNIKATTFEDIKILEALI"
     gene            complement(15781..16953)
                     /locus_tag="PLP02_00970"
     CDS             complement(15781..16953)
                     /locus_tag="PLP02_00970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPF42071.1"
                     /translation="MAYLFIYISLGAMFLLMVTSALAYHKFSVWKTILFFISGGAFII
                     MRFFPELVPFVSFDVIKPYYEYTLYLMVAIYAFTFKRKIRITKNLTDYDYFELEKELD
                     EIKTTSDLLRLRYISTIGLISEGLIFYDDDLTGLFITDQLSLLIGKGKNDLAMEEYAD
                     IVHKDDRAAYLSTIKKVSKKNPTYDMKYRIVRENVSIWIEEKGKAFEFDRKTQIISTV
                     KPIDIKLFPDTSIHEIDSLPSEQNLIQYLTQAMKETDPFYLIMIQLTNIPDINNRFGR
                     DVGNLMIAEYIKKMRFNFAKDVNSIFRITGIQFAIIIKDQKKYEVLHRALQSGGDLVN
                     LTLSIGGIQQVVYPNLGIIKREPWDTYTINELTGIANKSLAEAIHNPKKNYSVFGE"
     gene            complement(16978..>19283)
                     /locus_tag="PLP02_00975"
     CDS             complement(16978..>19283)
                     /locus_tag="PLP02_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42072.1"
                     /translation="VSALFLARKMRSVSVIIDGKENPDGDSEVEITDPENNKYRFAYD
                     SGAFVSGLNQNGLLEKYLHAIGIDTDLTGEVSEYDMVETTEGEFRKRINIFERFLVYL
                     VRYYPKQRDEIHRFFRDLEKHYENYVMQQEYMLKNTDYTLTSLMIEWGDYSLSALLGK
                     YFSDPLITNEFMMNCQLNGLNSDDVSSYNFFSGYFLGLKEGFYYLPVSVDGIKKLLME
                     KLKIINPKIIQHRRIKEYVGDVTGRITHVIDSTNKEIAAKHIIVGSKPDRFYKKYFPD
                     LTEHLELIDKFHPGLESPTRIRTVYLALNQKPHNIGITEMNYCFSCAGLAKPYITRLF
                     NYSLYEPTSTGAKKGALCVDYVYDENTEIGEQEILAQIYSKFPKLKKTIVGIKQGNPR
                     ELSAMVSLDEVRRGLTINEEIAIEASEHLMFFDNLHLTGSWVRPEAGLFGRFHTGVVL
                     GDKIEESLYYGENDDSFYYLTNDEIMVMLKHNYGRKTLGKLENHINFQIGKSSYYVRI
                     KDKNLTIHRGEYPHPDLTIYTTNDKLSNLLLKKVCFENVMESGGLKYTGSSELLYKVV
                     DAFELDDCKKTEVKFQPKLPVKNAGAKLMFVYLLAFSALAFLCNFIDMIWLVPFALGI
                     SLAMGYLKWRFFRRISWFEFSWAGIYLAMLFMAIFWQPFNVLHNDDMLLGLMGGMFFI
                     SWIINQPIVHDFRKYDYRADYAETKFFKTVNNGMTFVWALIFLLILGGVHVSGDRYVS
                     AWYNLVFLGIFLTYYYPIIYVSTNIKK"
     assembly_gap    19284..19383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19576..20319)
                     /locus_tag="PLP02_00980"
     CDS             complement(19576..20319)
                     /locus_tag="PLP02_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HPF42073.1"
                     /translation="MDLFILGTIVTYLYAALLLLLRTKVAHKTVRPAKIFLVLLLVLD
                     IFLLVKGNQSGTELFVGIFTMIPIPYMIVMVYINELRSTIKKIGMLHKKSRNPIINNE
                     LAEKLMDTVDFLSNRKVGALITIERSIDLSAFISKALMINAPVSSELLASIFVTTTPL
                     HDGAVIIRDNQILCAKAYYPSTERTDLPLHFGTRHRAAIGISEQSDALTIVVSEETGH
                     VSVAINRQLDYNVSRETLSLYFEKYLKIN"
     gene            complement(20396..20878)
                     /locus_tag="PLP02_00985"
     CDS             complement(20396..20878)
                     /locus_tag="PLP02_00985"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPF42074.1"
                     /translation="MKKLWLFVRKNFLTKKFLIFCLVGVINTAIHMSVYYAFYNKVLI
                     PVLSTGWSSFISNTIAFVSASIFSYFANAFFTFKPKNKTAMQFTAVMTVFLARLLISD
                     LLTTGFDFIIQDWIGLSYDQNSLYSIIAPFLASALLIPIAYFALDYVFHKTDRKKTTN
                     "
     gene            complement(20883..21797)
                     /locus_tag="PLP02_00990"
     CDS             complement(20883..21797)
                     /locus_tag="PLP02_00990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF42075.1"
                     /translation="MKKINIIVPAFNEEKNVRPFYDEITGFLERPGYEFHIMYINDGS
                     KDNTLNEIKKLASTDQRVRYISFSRNFGKEAAMHAGLEHSREADAVIIIDCDLQQPPA
                     LIPEMLEYYEEGYEIVYTKGKTRKGEPKLRTFFANCFYRIYNRHTEMPLDNGAKDYQL
                     LDCKVVEAFLAIKDRYRFVKGIFSWVGYKRKCLEYEFIPRKYGKSTWSFKKLFRYAFD
                     GMNQFSTLMMVVPAFVWFLSVAILIATALLFGFGLFTLAEFVIGLFVFIAVFALGFVT
                     YGIIYLLYQIRRQVLERPIYLIEETSEE"
     gene            complement(21794..24934)
                     /locus_tag="PLP02_00995"
     CDS             complement(21794..24934)
                     /locus_tag="PLP02_00995"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPF42076.1"
                     /translation="MKKGKTTAKENIVKTKQKIEGFFNNLTEKIKAQLYDVFSSKKKT
                     FAFLALVIFTVLFAIQMLIIFVRNSFYCNFSDDILQYYTIMVDFVAQIKAGTISFFNL
                     NNYLGASYYSDVYYIPLDIFTTITFILSYLMPTQLAYSVTELIKIWAGVMVFAYYLNL
                     KGMKNKTIFWMGLVYFVSGGTVSFMAFPAFLSLTFYLPLALIVIHWSFKGKKWIVPLF
                     AFVLVFYNFYLAYMALIFTGLMFIMEYVRREDFRFGKFFCDGAIFLGLLLLGVGMSLI
                     ILYPSILYVLQDTYRQTGTFNYWGIQIGSYELKLFEPEIYIRFLAKMFAEQYPTGFYG
                     FSQDYTKEHISLYITMTGLLMMSGIFFLKDRSSKVYKATILIGVLMAIFPFFSYLFSG
                     TTDQPYTRWINCYPLVMTIILAHVFDKTGFEKLNMKQLTIPAVLLMGLDIYLVYYYIH
                     KLDLLRDFKFADVLTLDWAMMIVSAVVILLFLIFGWMKKPGWLKCLFVAECCVALANI
                     YGNAFFIGNKNDTFEEMYSINDVLKEQLAEDGESFYRVYVEMQAFNVEDTNFNRMTVY
                     PTNTEIFHSWTDYESDTLATLLFNLSTNAEEHQSKSKLNTMALFLNQFLGYKYVLTST
                     EHAYYLDSDYFELIYSDSEYVLFRIVNAENFQVYESYASYSEYEGFCDFNNNLEAQKI
                     FLMAALIDTERYEDMTFNLEEIMLDDRSSENTIYPSKTITSYELVAFEGSGDDYNPSG
                     LFFRFDGSQLNIGFDAGALYINVNSGIYSGDVYMEFADGSMYKSQFPASDYYDVKCEF
                     GSEPVAIYFETNGLNTRLSITYRMEMARDSSAYLVFDLSGIEYTDGAGMLYLRMSQKL
                     GTVIFVDAEGNETYGFKDYYYFDSAPVRVYIFKTGNMYSNVNNFFQFSLHYVYDGDYL
                     EEYENLTNGNKLYANGSLEITNGTISLSYLRTSETTYDQIVVIPVTYSEEWTVTSEQQ
                     YETISASGGMLGIIVPAGIEDVSIELRFIPKGLKEGALGSLAFIAVYLGIFLPGWISE
                     KKKRNKEQISKEMNES"
     gene            25068..26309
                     /gene="hflX"
                     /locus_tag="PLP02_01000"
     CDS             25068..26309
                     /gene="hflX"
                     /locus_tag="PLP02_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPF42077.1"
                     /translation="MEKAILVGLDLGTDSDFFASLAELKNLAEACDVEVVGQVTQKAD
                     APTANFYIGQGKVAEIKQEINVLDANLLIFDDELSPSHIRNLEQALEIKIIDRTVLIL
                     DIFAKRAKTKEAILQVELAQAEYYLPRVAGMYKSLSRQKSGTGSKGPGEQQLELDKRI
                     IRNRVHKLKTELNELVAVRRTQRHKRSTSNVPTVAIVGYTNSGKSTLMNALMSHAEKK
                     SDKYVFEKDMLFATLETQTREIKRDNNHDFLVTDTVGFISKLPHQLIEAFKSTLEETT
                     EASLILHVIDASNPRHYEQAKITETVLEELGAKNIPIIHVINKMDLCPEIPANGFSNP
                     VYVSALKDENLDRLLDMIDKTLFGNRHLVKLFLPFDKGGIYSELQENAFIKSTEYTPD
                     GISVLVELSEALYKKYQEYIA"
     gene            complement(26382..27368)
                     /gene="dusB"
                     /locus_tag="PLP02_01005"
     CDS             complement(26382..27368)
                     /gene="dusB"
                     /locus_tag="PLP02_01005"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPF42078.1"
                     /translation="MDMEWKVAGQTILNQVVVAPMAGITNPAYRRILKECGAGLIYTE
                     MVSDKGLGHENEKTFSMLTVMPDEHPIVLQLFGSDPESMAVAAGIVTNETDADIIDIN
                     MGCPVTKVVKNGAGAKLMLDIEKASAIVRAVVSVTNKPVSVKIRSGWDKEHLNAPSFA
                     KALEESGASMIAVHARTKTEMYGGKADWDVIRQVKEKVNIPVVGNGDIRKPEDAEHML
                     KQTGCDAVMIGRGLLGNPWLVSQTVEYLETGTYEPHVTLEKRKEMIYRHFELLRDLKG
                     ERSAILEMRSHGPWYLRGLKNSAQIKARLSQTRTEKEFSLVIEEYFDYLYSL"
     gene            complement(27684..29024)
                     /gene="glnA"
                     /locus_tag="PLP02_01010"
     CDS             complement(27684..29024)
                     /gene="glnA"
                     /locus_tag="PLP02_01010"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPF42079.1"
                     /translation="MAKYTKEQILRNAEEENVRYVRLMFTDINGTIKSVEIPVYRLKS
                     ALNGEVMFDGSSIEGFVRIMEADMYLRPDPDTWLILSWEETAYGKVARLICDVYTPEG
                     KPSEADPRGNLKRVAKKMEALGFSTLNIGFEPEFFLLKLDEKGEISMKVTDEGGYFDL
                     SPIDGAGDCRRDIVLELERIGFTMEASHHEVAPGQNEINFQFSNIVEACDNIQTFKLV
                     VKNIARRHGLHATFMPKPIAKINGSGMHTNCSLANMNGENAFYDPKDPMGLSLVCRQW
                     LTGILTHSRHFCAITNPTVNSYKRLVPGYEAPCYVSWSEHNRSVMVRIPATRGRTTRT
                     EIRSVDTSANPYLAMAVILASGLDGIEKNLPLIAPINENLFEKTDEEREALGVASLPN
                     NLKEAIDLLKEDELLKDALGEHIFKKFVELKIREWDEFRTTVTEWEIKKYIKLI"
     gene            complement(29156..29716)
                     /locus_tag="PLP02_01015"
     CDS             complement(29156..29716)
                     /locus_tag="PLP02_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42080.1"
                     /translation="MKFRRILACVNDGLLAIRIQNILRANGLSPDIASHPIQSDEAGR
                     YDIVVIHESYRITDLYGFTENIVTRKNIPVVFLSSNPAGAILRRLSSYPGFMLADEMK
                     MDSELPVILVMYDKYLEKNKALENENRKAKSKIANGKLFQQCKEYLISTGMSEDEAHK
                     KILKTAMDEKIGKYDAALKILQKKTK"
     gene            complement(29713..30957)
                     /gene="glyA"
                     /locus_tag="PLP02_01020"
     CDS             complement(29713..30957)
                     /gene="glyA"
                     /locus_tag="PLP02_01020"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPF42081.1"
                     /translation="MDKLKTVDPEIAALIEEELGRQQSHLELIASENFVSEAILEADG
                     SVLTNKYAEGYPGKRYYGGCEYVDGVEDLARERAKKLFNVKFANVQPHSGSSANMGAY
                     RAVLEKGDVVMGMALDQGGHLTHGHPLSFSGIDYEFHPYFVSKETETIDYDELEKTAL
                     EIKPRLIVAGASAYPRIIDFARLRKIADETGALFMVDMAHIAGLVATGLHPNPCDYAD
                     IVTTTTHKTLRGPRGGMILTNNEEIAKKIDRVIFPGIQGGPLMHIIAAKAVALQEALK
                     PEFTVYQKQVVANADAFAKRFIELGYHVISGGTDNHLLLLDVVSKSELTGKKAEKLLD
                     SVNITCNKNTIPYDKEKPFIASGIRLGSPAMTTRGFKEADFIRVADLIDMALMNPENE
                     EILAKVKAGVKDLTDSHPLYRR"
     gene            complement(31060..31917)
                     /gene="rsmA"
                     /locus_tag="PLP02_01025"
     CDS             complement(31060..31917)
                     /gene="rsmA"
                     /locus_tag="PLP02_01025"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPF42082.1"
                     /translation="MKDKTTAMGVKPKKRFGQNFLSDRNILNKIIDAAKIDKETDVIE
                     IGPGTGNLTELLAKKAGRVVAYEIDSTLIPILKERLKSLDNITIMNKDILEIDIDKEI
                     DEHFPDSKKIMAIANLPYYITTPILMKFLKTSDKVTRLIFMMQLEVAKRLSAGPGGKD
                     YGALSVIMAYRTKTEIRFKVPRTVFVPAPNVDSAVVDIEIRNKPPFIVKDDSFFYDFI
                     HKMFAYRRKTLSNNLATAYPETNKTETDSLITKANLAPGVRAEALSPEEFGRLSDIVW
                     DALGKSKTA"
     gene            complement(31919..32839)
                     /locus_tag="PLP02_01030"
     CDS             complement(31919..32839)
                     /locus_tag="PLP02_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPF42083.1"
                     /translation="MKKTMMIYNPASGKQKFVGRLEETLARFRAAGYDIEAYATGKPG
                     QATILAREACQKGYEEVIIVGGDGTFNECVNGLMEFENRPTIGYIPAGTCCDIGMTLG
                     LTKNIDKAVNNILTNTPVKMDVVKANDRYFCYVSGNGAFIDISYVTDSKLKKKIGYFA
                     YVIKAAEEIFTIPKMRMRVTHDHGTVSGVYSLVLIINSKRVAGINMIYKPSLDDGLVN
                     VVMYKSLLPLNWILYILSFLLPFWSTPLVTRFKTSSLKIVTNTRSRWNIDGESGGVGN
                     QNIHVCRHGISIIVPPATKKRYFHHQDESV"
     gene            32959..33615
                     /locus_tag="PLP02_01035"
     CDS             32959..33615
                     /locus_tag="PLP02_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPF42084.1"
                     /translation="MRKIEAVLFDLDGTLVDSNNIIIDSFRHVMTAFFPERLWSRKNI
                     MEMIGPPLYDTFKKHASSEEMISDMIFAYRKYYVENEFDSISLYPGVKETLTELKNKN
                     CRLGVVTTKFRVSALPSLEHFGLDKLFEEIVSLDEITHAKPHPEPVLYALKQLKCHFG
                     IMVGDHPSDIVSGKAAGIATCAVDWSLKRKELELSSPDYWIKDFRQLIKIVDDINKEE
                     "
     gene            33612..34028
                     /locus_tag="PLP02_01040"
     CDS             33612..34028
                     /locus_tag="PLP02_01040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPF42085.1"
                     /translation="MTMDCIFCRIANGEIQSYKVYEDDCFIAILDISQATKGHTLLIP
                     KQHFANVYEIDEEIIARIFKKIPEIAKAIKKAFNPIGLNILINTDKPLQSVFHFHVHL
                     IPRYENDGVRMEFENNYGKLPAEEFLKIADEIKKNL"
     gene            complement(34060..35337)
                     /gene="serS"
                     /locus_tag="PLP02_01045"
     CDS             complement(34060..35337)
                     /gene="serS"
                     /locus_tag="PLP02_01045"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPF42086.1"
                     /translation="MLDIKQIRANPELIIGKLNRRGGDFSYLYDMLETDRKRREAILE
                     VEALKAKKNEISKQIGQFKREGKSVESILKNMDDFSHSIAGLEDAVRQYDDDIRETLL
                     KTPNMPADDIPNGKDDSENVEIKRNLEPTQFDFVPKSHYDLGEALDILDFQRAGKISG
                     ARFVVYKGLGARLERSLIQFMMDLHANKHGYTEIMPPYLVNETSMYGTGQFPKFMDEA
                     FKVNDDRNLYLNPTAEVPTINLHRDEVIDKDLLPIKYVSYTTAFRKEAGSAGKDTKGI
                     IRQHQFNKVELIKFTKPEESYKEQMRMLEDAEDVLKLLKIPYHVVVLCAGDLGFSMCK
                     TYDIEVWLPSENRYREISSVSNAEDYQARRANIKYRPDTDGKLEYVHTLNGSGLAVGR
                     TLVAVMENYQKADGTIEVPEVLRKYMGTDIISK"
     gene            complement(35359..38022)
                     /gene="gyrA"
                     /locus_tag="PLP02_01050"
     CDS             complement(35359..38022)
                     /gene="gyrA"
                     /locus_tag="PLP02_01050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPF42087.1"
                     /translation="MENQEYENTNIKDKIQEVNISGEMKTSFLSYAMSVIVARALPDV
                     RDGLKPVHRRILYGMDELGVYPDKQYKKSARIVGDVMGKYHPHGDSAIYDSMVRMAQD
                     FSYRYPLVNGHGNFGSVDGDGAAAMRYTEAKMEKITVEMLKDLRKDTVDFVDNYDGSE
                     QEPKVLPARFPNLLVNGANGIAVGMSTVIPPHNLTEVIEGYIAYIRNQDIEDEELMEY
                     IKGPDFPTGGIILGMSGIREAYLTGHGTIRVKAKAEIKDMASGKKQIIIKEIPYQVNK
                     SNLIDKIAELAKDKRIEGITDLRDESNRNGMRIVLELRKDANPEVILNNLYKNSQLQA
                     SYGVNMLALVDDKPMILSLKEIIKYYFEHQTTVLIRRTKFDLDKAIQRDHLLKGFIIA
                     LDNIDEVIKIIRESYDDTEQKLIERYGFSDVQTKAILAMQLRKLSGMERDKIHAELAE
                     IEKAINYYNQILVDKELQHEILVSEAEDVKNKYGDARRTEIDLSGDYDIEDEDLIPIE
                     DVIVAVTTNGYVKRMNVAVYKSQNRGGRGKTGMKINEDDVIDSIISTSTHDYLLFFTN
                     LGRVYKIKGYRIPNYGRNSKGLPIVNLLNLTDGEGLAAVMSVKDFNEGYLFFTTTKGV
                     VKRTKVSAFQNIRTNGIRALSLRDGDQLHSVKLTDGNRDVIIGASNGKAIRFNEQDVR
                     SMGRDAAGVKGIALSGKEEVIGVTTVTVDRSEILAITAKGYGKRTDVDEYRLQGRGGK
                     GVKTINVTTKNGCLKKLISVVGDEDLLVVTDKGMIIRVPIDQIAQTKRSTQGVKIINV
                     LDGHEVATIAIVPKEEDDDENMESIDDNSEITEETNDNISANQEQQKPIREFHMEEYK
                     ELNHFVEEDEEENEEDDEEESDVDDILKDSY"
     gene            complement(38032..39945)
                     /gene="gyrB"
                     /locus_tag="PLP02_01055"
     CDS             complement(38032..39945)
                     /gene="gyrB"
                     /locus_tag="PLP02_01055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPF42088.1"
                     /translation="MADHNNAKYDASNIQILEGLEAVKKRPGMYIGTTGPRGLHHLVW
                     EIVDNSVDEALAGYADRIIVDILPGNIVRVEDNGRGIPVDIHPKTKRPSVETVYTVLH
                     AGGKFDHNSYKVSGGLHGVGASVVNALSEYMDIEIHKDGKIYTISFDHGFLKKELECI
                     GDTDKSGTIVTFLADPKVFVECQEYDFETLRSRIQQLAFLNKGIQFDINDLRNPQSTV
                     RKEYKYEGGVREYVEFLNLGKAVLNPQVAYFEGEQEGITVEIAMQYNDGYAANIYPFT
                     NNINNAEGGTHEEGFKMTLTRILNAYGKSANVFRKDESLAGEDTREGLVAIVSVKHPD
                     PQFEGQTKSKLGSSDARRVVANIMSEGLDRYLLENPAAAKNILEKALMAQRARIAAKK
                     AREATRRKSPLDSLGFASKLADCRTKDAAKSEIYLVEGDSAGGSAKQGRDAEFQAILP
                     LRGKVLNVEKARLDRALQNKEIVSMIQAFGTGIGEEFQIDQARYHKIIIMTDADVDGA
                     HIRTLLLTFFFRYMRPLIEKGYIYIAQPPLFKIQLGKSIQYAYEEKELERILAGLTGK
                     PVLQRYKGLGEMNPEQLWETTMDPEARTLLQVTLEDAIEADRIFSMLMGDDVPSRREF
                     IQENAEYVRNLDI"
     gene            complement(39947..41035)
                     /gene="recF"
                     /locus_tag="PLP02_01060"
     CDS             complement(39947..41035)
                     /gene="recF"
                     /locus_tag="PLP02_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPF42089.1"
                     /translation="MKIDKVTLTNFRNYHKQTLELGSGIHFIIGANGEGKTNFLEALY
                     VLALAKSYKAEDSDLIRFSADFARIQATIEKDGRKFELTMIITEIGKKAMRDSQEIKR
                     LSDYIGNLNIVSFLPEDIVLIKGAPRDRRYYVDVFLGQIDKDYLNELTIYKHILKQRN
                     ELLKKLEYVEKPDYTLLDVITEQLASSAEKISQRRVRFQEEINGLIDGMYKRLSDKDE
                     KFSLVYQQTLPQKEAYGFLKEKYRQDIMQGATGYGPHRDDYVFMLDEFAAGSRASQGE
                     QRMMVLSLNMGLAELVKETRHETPVFLLDDVFSELDETRQNRLLEYVATSGIQTIVTT
                     TGLGGVKDFILKQAHVFHVNKGYIKEEY"
     gene            complement(41032..41250)
                     /gene="yaaA"
                     /locus_tag="PLP02_01065"
     CDS             complement(41032..41250)
                     /gene="yaaA"
                     /locus_tag="PLP02_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003592392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HPF42090.1"
                     /translation="MKTINIKTPFITLGQLIKYSGLVQSGGDAKIFLEENKITHNGAL
                     ENRRGKKIYPGDLVEIENKIILKVVNKE"
     gene            complement(41409..44882)
                     /gene="mfd"
                     /locus_tag="PLP02_01070"
     CDS             complement(41409..44882)
                     /gene="mfd"
                     /locus_tag="PLP02_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPF42091.1"
                     /translation="MMEKILEFLEAKKEFKEFSEAIKQQGKAIQISNTTEEGGMFLVL
                     SAHKQAGGPIVVVTPNLYHAQIVYDKLSAALDPEEVLFFPQDEFITSEMLVSSWEFRI
                     ERINTIKRLIENNNAIVVTHLPGLIKPQLEPEAWKNAMFSLCIKQEISIDKLSSKLIM
                     SGYSHEYTVEKMGDFSVRGGIVDVFPLGEKTPVRLDFFGDEIDTMKRFDIESQRSQES
                     CEKIDVMPMTELFFDDKGLKTIKNEVKTIIRDKKLSSEAIEKIDADLESLERREDLER
                     LSRYQKFLFGQQNTILDFIPKSTVFFFDFHRFNGQYDGLKQDLLDWIENAGDYAKMGF
                     DFFVPLENIKIEKPIYVDYLREETRLQNIKKISIAANEPLKFEGNINLVLNELRSLAG
                     QRTLVIALESYKSRTEMCELLSDNKLDYVLTGDTERLKENSINIIVSDRCFDFASEAF
                     GLTVITEKSINKKQTSKRKGKYLSAFDSSKRLASVNDLKPGDYVVHYDYGIGRFLDIK
                     TMELGNTRNDYINIEYRDGDKLYIPLESFSDLQKYAGSEGFVPRLSKLGGSDWNKAKQ
                     RVRSKAGDIAERLIRLYSQREKAKGYSFRPNAELESELEIDFPYEETRDQNKAIKEVF
                     SDMESPRPMDRLLCGDVGFGKTEIALRAAFRAVLNNKQVAYLAPTTVLSKQHFQTFTS
                     RLEKYGVNIVLLNRFVTGKAQKQILTGLENGSIDIVIGTHRVLGKDVAFKDLGLLIVD
                     EEQRFGVQHKEKIKEFKVNVDVLSLSATPIPRTLQMAIMGVKSMSLLESAPENRYPIQ
                     TYVIERNDAVIKDAIERELAREGQVYYLYNRVDDIENIAIKIQKLVPEARVCIAHGQM
                     HKLELEAVIDDFIDRKTDVLVATTIIETGIDIPNVNTLIIHDADKLGLAQLYQIRGRV
                     GRSDRIAYAYLMFKKDRVLTEDAEKRLKVIKEFTELGSGFKIAVRDLSIRGAGDVLGS
                     EQSGFIDSVGVDMYMRILEEEINAKQGKPEIKETKPGIKATVSRYIDEEYINDDFVKI
                     EMYKKINSIKNKKDIADLLAEFKDRFGEYIPELEIYLYEKLFEKLSKMIDGEKLIESK
                     TNVTLVISEDGTKKMSGEALFTTGLEISNYIRFAYRHNKISIIVDTVGLEKHWLYTMV
                     DFLEKIVSENK"
     gene            complement(44952..45716)
                     /locus_tag="PLP02_01075"
     CDS             complement(44952..45716)
                     /locus_tag="PLP02_01075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF42092.1"
                     /translation="MEGKPFVLITGASSGIGRELARGLAKKGYNVILVARRAERLEEL
                     KIELTSNYRTDVVIKAMDISLPENTQKLYDETITYKPLIVINNAGFGQYGSFEEIPPE
                     KESEMIRLNIDSLSRLTKLFASGMERGLILNVASMAAFLPTPLMAAYAASKAFVLNYS
                     LAANSELVQQKKPVRIKILCPGPVETEFADVARVKTAFKGITPKKCAEIAIRNLDNKK
                     TIIIPGTLMKIARVALRVFPKKTVMAISYRYQMKKR"
     gene            complement(45821..46363)
                     /gene="pth"
                     /locus_tag="PLP02_01080"
     CDS             complement(45821..46363)
                     /gene="pth"
                     /locus_tag="PLP02_01080"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPF42093.1"
                     /translation="MIVGLGNPGRKYAKTRHNVGFMCLDAYAKTKKAKFSKETKFMGE
                     VLTIGETIFLKPKTFMNLSGNSVKAVAGFYKIPVEEIMVISDDMDLPLAKIRLREKGG
                     SGGHNGLKSIISQMGTEAFKRVRIGIGHSEENDTVDYVLSGFSKAESKMLDDTIIECM
                     GIIDDFQNGKPFEETMNRYN"
     gene            complement(46374..46994)
                     /gene="jag"
                     /locus_tag="PLP02_01085"
     CDS             complement(46374..46994)
                     /gene="jag"
                     /locus_tag="PLP02_01085"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPF42094.1"
                     /translation="MKSIQFEAKTLNESTYKYAAEELHVNRKYVDLQVVEEKKSILGL
                     NKTYIVEAKIKFDPLRDGIEYLKEILTAMEIEAIVEAKAEEERQLTFSIDAKENPLLI
                     GKNGKTLDAVQTLIKNFINLFTDEYYVVSVDVGGYKNQKKKQLEILATKTAKEVARTK
                     VPAKLGKMNAYERRIIHTKLADWRDVITESEGEEPNRYVVIKPKNK"
     gene            complement(47011..48102)
                     /gene="yidC"
                     /locus_tag="PLP02_01090"
     CDS             complement(47011..48102)
                     /gene="yidC"
                     /locus_tag="PLP02_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPF42095.1"
                     /translation="MKKTKIIAAILLFVLVFALSACGSKAEVSYENYTSIVTVSEDET
                     NATSYYEAINLLGTPSDESGYANGDGVVVWSTDTATVTVTFTTYKAVSKTQEGLSASG
                     TLLPIGEKDSWTEWVVMVVAKFTFYASNLFGLLGETYYYWLGLLIMTLVIRTLAWPIY
                     SKSNDMTLKMQIAQPEITRIQEKYKGRSDQASQQRMQMETMEVYRKYKINFFGCLMPL
                     LQMPIFIAMYTVVRRFPITPVSAFGNTDAIMNYNFLWTDLGNTGFWENLPLALVVVAT
                     MMLSQWLIQKQTRKNQKVNKYQNSQAQQSQKMMKYMNYFMVLMMGYIAISNAGIAFYW
                     ILGNSYQLLQSYLSRRNIESKQEKMRSKF"
     gene            complement(48117..48443)
                     /gene="rnpA"
                     /locus_tag="PLP02_01095"
     CDS             complement(48117..48443)
                     /gene="rnpA"
                     /locus_tag="PLP02_01095"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPF42096.1"
                     /translation="MEKAYRIKKNQEIDALVKQRNSAANGFFVVYAAVNKIDHFRFAV
                     SVSKKYGNAVERNKMKRRIREITRQANITAKTDFLIVAKPTARKLDFKEIKDNLTPLF
                     VKAKIT"
     gene            complement(48530..48688)
                     /gene="rpmH"
                     /locus_tag="PLP02_01100"
     CDS             complement(48530..48688)
                     /gene="rpmH"
                     /locus_tag="PLP02_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPF42097.1"
                     /translation="MTKRTYQPSKIKRIRTHGFRARMESANGRKVLARRRAKGRYSLT
                     VSDQSTSK"
     gene            49079..50455
                     /gene="dnaA"
                     /locus_tag="PLP02_01105"
     CDS             49079..50455
                     /gene="dnaA"
                     /locus_tag="PLP02_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPF42098.1"
                     /translation="MEEFQKIWDKIKYQLQLTLDEDVYNEHFAPISNVFKVVNNYVYL
                     ISPNSFIKSKIESFYLNRLNAMLNEHVSEKHMFKIITKEQAAEEIAKTSTYEINIPEQ
                     GLENKYKTGLNNSYTFDNFVVGSSNRLAYTSAMKVADQPGEVANPLYIFGDVGLGKTH
                     LMQCVGNYILENNVNKKVLYVKTDQFIEDYAKAAQKKSFDDFSKKYEDVDILLVDDIQ
                     FLSGKTQSQLEFFKIFEKLKENNKQIVITSDRQAKELYDIMSRLTSRFEWGLSVDINR
                     PNLEHRILILKKKLQAETADPELIPEEILDYIASIFDNNVRELEGALKRVLFYCTAFD
                     YKFTINNAQDALKNLVNSKNISNNSIERSNFNNVLDIVSSYYNISTVDLLSKKRKKQY
                     VFPRQIAMYILKDLYDLTYKKIGQIFNNRDHSTVIYSIEQISHDIQTDNTKKTDIDKL
                     LIKCGKKG"
     gene            50608..51732
                     /gene="dnaN"
                     /locus_tag="PLP02_01110"
     CDS             50608..51732
                     /gene="dnaN"
                     /locus_tag="PLP02_01110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPF42099.1"
                     /translation="MNFIISTSVLLNNLLIAQKALSTKTPAPYLQGIKMEVYQKEVVL
                     VTSNSDISIKLSIKDESLKVESTGEALVPGRFFVDIIRKVEGETAELSVIDNNILRIV
                     AGESDVVLNLLNIEDYPELEFPLNDEPIKLDSKIMKAIIRQTTFATSTIENRPILTGV
                     NIRVEGEKLIAIATDSFRLSQKVVDIPKNMDNMNVVIPGRSLDELAKILEIDLEEILI
                     HLNHKSILFEYGNVLFQSRLLEGNFPETSRLIPMEFPIVIKFNRDKLYVAVERASLLS
                     SKEGTSSIVKLNLRNDNIVEITSNSPELGKVMEKVYPIEKAVGMTIKIAFSSKYFMDA
                     LKTFNSEEIFVKFTGEIRPFVLEGDNDPGLIQLILPVRTE"
     gene            51918..53153
                     /locus_tag="PLP02_01115"
     CDS             51918..53153
                     /locus_tag="PLP02_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF42100.1"
                     /translation="MQNQIAGRHAANKKLESNILQISQEAKAMKKKYDDVIDATVGML
                     HHEEGNVFKYQIVEDLLKGLTDAETYHYAPVRGTPAFSEGAFDWVFGKNAETIRKNFY
                     HAVIPTTGGSGAISDTFYNFNDFGQKVLIPNHYWTPYQNFAYEANLGLQTFSMYDDMK
                     RFNVAAFKEAALNLAHEQNRLSVLLNDPCNNPTGLCMTREDWDKVIEVLNQIASEGIP
                     VTLMQDIAYVDYQMKPVEMSRDIFLSYLNLHPDILTVVIFSGSKTFSLYGFRVGAQIG
                     LSKNKDVLDTFVNVGDYSVRARFSSVNQPGMSIIGKIFSNPENKQKFQDELVIGRKLL
                     RDRAVLFMEEADKVGLDMLPYCGGFFISVLTDNKNIFQDLASDHVYVIHMDNLIRIAI
                     SSLPLTDIPRLVRILKKHV"
     gene            complement(53183..53398)
                     /locus_tag="PLP02_01120"
     CDS             complement(53183..53398)
                     /locus_tag="PLP02_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42101.1"
                     /translation="MKEYVRTIDGLPLIIKVILALPFIDGIIYGIYRIAKGRLIIGIL
                     WIIFGACILWLVDLISILLKGKVVFLV"
     gene            complement(53468..54484)
                     /gene="aroF"
                     /locus_tag="PLP02_01125"
     CDS             complement(53468..54484)
                     /gene="aroF"
                     /locus_tag="PLP02_01125"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPF42102.1"
                     /translation="MIIKFKEKAKPEEIALLRDVLQNQGFSIHESTGTSITLFGVIGD
                     TSKFDPNSLYVYDFIETVLRVQEPYKQVNRKFHPEDTIINVKGVQIGGKNVVALAGPC
                     AVESLEQMQSIAPLVKAAGAKILRAGAYKPRTSPYSFQGLGEKGLKILGQIGREYSLP
                     VVSELMSIEDIPLFIENVDIIQIGARNMQNFSLLKEVGKLDKPVLLKRGMANTIEEWL
                     MSAEYIFAGGNDKVILCDRGIRTFETSTRNTLDISSIPVLKKLSHLPVIVDPSHAAGR
                     WEFIESMSRAAVAAGADGLIIEVHPDPEKALSDGQQSLKPERFSTLMQSCRLVAGAIG
                     RTME"
     gene            complement(54481..54918)
                     /locus_tag="PLP02_01130"
     CDS             complement(54481..54918)
                     /locus_tag="PLP02_01130"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPF42103.1"
                     /translation="MDILIINGPNLNLLGLREPAIYGKASYSDLLGYLSGYSALSGDN
                     LFFFQSNHEGSIIDFIQENYTKASGIVINPGAFAHYSYAIYDCLLSVPIPAVEVHISD
                     IYSREPFRQNSVISPACIGTICGKGFSGYTEAIELLKKGSRTK"
     gene            complement(54890..56152)
                     /locus_tag="PLP02_01135"
     CDS             complement(54890..56152)
                     /locus_tag="PLP02_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPF42104.1"
                     /translation="MYGLLGKSLSHSRSPEIHRLLGNADYRLLESPDPESFLSSKLFT
                     GLNVTIPYKESVIPLLDELDPVARATGAVNTVLNNNGRLVGYNTDVFGFEQLLVHAGI
                     NPYQKKVLILGNGGAAKACAFVLHKLGAKQTVKLCRRIKSENEFLFSEIEKVVDSEII
                     INTTPLGMYPENDSSVLIDLNLFYRLDSVIDLIYNPLHTNLLLDAKSKDIFATNGLYM
                     LVSQAAKSNELFTGIPATKNQIDSVFSILSAQMTNLVLIGLPGSGKSSVAGLLAKGLK
                     KTVADCDQLISDAAGMSISDIFASFGEPYFRNLEYELTKGLYQNQGLVISTGGGMVEN
                     DNLMRLLRQNGLLVFLDRPITFILKESVPGRPLLKTPEDLLALEKRRRSLYEKYADIR
                     YESVSNDPASEAALIKERFYGYFDNQRA"
     gene            complement(56152..>56762)
                     /locus_tag="PLP02_01140"
     CDS             complement(56152..>56762)
                     /locus_tag="PLP02_01140"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPF42105.1"
                     /translation="LFANDMLNNEFLEKMKTSGFPVISDRVSELMKQEIENAKKSGDS
                     VGGIVETVIVGLPVGIGEPLFASCESVLSSNLFSIPGIKGVEFGSGFAITEKLGSEAN
                     DPYRIQDGKIITTTNHNGGIVGGISTGMPLVFRVAVKPTPSISKEQKTVDLSSGTNSE
                     IEVSGRHDPSIVPRVIPVISAVASFTILDLLLLSRIGRSVAK"
     assembly_gap    56763..56862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<56863..57842)
                     /gene="aroB"
                     /locus_tag="PLP02_01145"
     CDS             complement(<56863..57842)
                     /gene="aroB"
                     /locus_tag="PLP02_01145"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPF42106.1"
                     /translation="METIIIKSRFFSYPIHVENGILSRLDEFWDVTRQVVVISDDGIP
                     VSYVDTVVNKFPGCLKIIFPQGETSKSLQQYERIVGLLSANHVSRDALLVALGGGVTG
                     DLAGFVAATYLRGIEYVQIPTSLLAQIDSSVGGKVAVNTKQAKNIVGCFYPPKMVLVD
                     PSLLSTLPERQFRNGLGEMIKYGMVADSLLFEKLENPVLPSELNFLITRSINIKKRFV
                     EQDEFDTGSRQALNFGHTFGHAIEAFYGYEKYLHGEAVAIGMMRIVSNATIKQRLENA
                     LRSQGLPTADEASNELLWDYVMKDKKNRAKALTYVDVNAIGSYVLKTAKS"
BASE COUNT        14826 a        13103 c        11765 g        17667 t
ORIGIN      
        1 cgaccgcctt tgacattgaa agagaaccgt cctttgtcat atccacggat cttggcttct
       61 ttggttttgg caaacatttc cctgatctcg tcgaatacac cggtataggt tgccgggttg
      121 cttcgcggcg tgcggccgat ggccgactgg tcgatattga tgatccggtc aaatgcttca
      181 tagttgccga tgcctttgca ggttccggcg ttttcgtttc ttcttgtgat cagattgaca
      241 aggttcttgt aaagcacttc gttcactagc gatgatttcc ccgatcccga tacccccgta
      301 acgaccgtga gatttccgat cgggatatcg acatcgatat tttttagatt gttctgggta
      361 gctccgatta ttttcagata gccgcgttcc gcttttcttc tttcttttgg aagcgggatg
      421 gacattttcc ctgtcagata ttttccggtg atcgaatttt cgtctttgac aatatcctta
      481 ggtgttccgg caaatatgac tttgcctccg tgttcgccgg caccggggcc catgtccacg
      541 acaaagtcgg cgttttcgat catttccata tcgtgttcga cgacaatcat ggtgttgcca
      601 agatcgcgca ttcctttcaa cgtgtcaatc agacgaagat tgtcgcgttg atgtaatccg
      661 atcgatggct cgtcaagcac gtaaaggacg ccggtcagac gtgagccgat ctgagtggct
      721 agtcggattc tttgggcttc gccaccagaa agcgttccgg aagaacggga caatgtaagg
      781 taatcgagtc cgacattcat caaaaatctt agtctttcct ttatttcttt tagaatcggt
      841 ctggcgattt ctttctcgcg gtccgtaagt tccaacgtct cgataaaatc cagaagatgt
      901 aaaatactca acgatgaaaa atcatcgatg ttcagtccgc cgacttttac cgagagcgcg
      961 gcaggattca atttttttcc accgcatact tcgcatggaa tatcattcat cattgtttcg
     1021 atccagtcgc ggatcattcg cgatgttgtt tccaaatann nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnga tttgccggta tccggtatca gaagattcgg atcgatcttt
     1201 cttttaaatc ctaatcccga acattccggg caggctccgt atggagcgtt gaaggaaaat
     1261 aggcgcggtt cgagttttcc gatcgagaac ccgcaaatag gacaactgaa atgctcgcta
     1321 aaaatcagtt catcctcgtt cgccaaaact ttcaccaacc catccgaaag tttggcggca
     1381 gtttccaatg agtcatataa acgtgatctg actcccggtt tcacgaccag tctgtcgatg
     1441 acgacatcga tatcggatct tttgtttttt tccagatcca tatcctcttc gagatcatgg
     1501 aattctccat tgatgcggat gcggttatag ccggtgcgcc tgagttcttc cagtgttttt
     1561 tcatgcatgc ctttctttcc gcggacaacc ggactcataa ttgtgagctt ggcattttcc
     1621 ttttccaaca atctgttggt catttcctgg attgtttgcg atgtaatcgg aatcccgtgt
     1681 tccggacaat aaggaacccc gatccttgca taaagcaagc gaacataatc gtatatttca
     1741 gtcactgttc cgacagtgga acgcgggttg tgcgaagttg tcttctggtc gatcgaaatc
     1801 gccggcgaca atccttcgat cgaatcgaca tccggctttt ccatattgcc gagaaactga
     1861 cgggcataag ccgataatga ttcgacatac cttcttctgc cctcttcgta aattgtatca
     1921 aacgccaaac tggtctttcc cgaaccggaa agacccgtca tgacaatcaa ttgattttta
     1981 ggaagaacca gatcaaccga ttgaagattg ttttccctgg ctcctttgat gataattttg
     2041 tcgttcatat tttgaaaagc tcccttatat tcatgatgcc gcaatttctg taatgattat
     2101 accatataac ggttatgatg aaaaaagaat cgcgcctacg attcttcttc caacagacgg
     2161 ttgaattctt gctcgtcgat gatcctgatt cccagttctt ttcctttggt cagtttcgac
     2221 cccgcatcag tcccggcaat gatcagatct gtgttatggg aaacggaaga agagacgttt
     2281 cctcccagac tttcgatcct gtctttcgct tcgctccttg agtaaccttc caatgcgccg
     2341 gttaaaacta ttgtcttacc ggaaaatgac gatcccgtac tcgtaaattt ttttgatgcg
     2401 accatattaa ggcccagatc ttcaagttca tgtatcattg ccgcattttt gctttgttgg
     2461 aaatattcgg tcagactgga agcgatcgcc tcgccgatat cggacagttc cagcagatct
     2521 tccttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccatc aatgttgtat
     2641 gcatcccggc ttgcgaaatg aataagtcct tcgattgact tacctggaca atccggattc
     2701 atacagtaaa tatcaacttc gttttccttg cgggaaagcc ggcttttgca gatgggacaa
     2761 gtctctgcca taatgaaggg tttttccctg ccgtcgcgtt tttccggtat cgatcctaaa
     2821 atctcaggaa ttatctctcc ggcttttctg actataacgt gatcgccgat tcgaatgttt
     2881 ctggccttaa caaagtcctc gttgtgaagc gtcgcgcgcg ataccagcga accagatatc
     2941 atcaccggtt ccagttctgc gaccggcttg acaactccgg ttctgccaac ctggaagcga
     3001 atatctttca aaacagtcac aacttcctcg gccggaaatt tatatgcgat ggcccatttg
     3061 ggatacttgg cggtgtatcc gatgcggtca tagagattat actcgttgac tttgatgact
     3121 attccgtcga tatcataggg aagttcgttt cttttatccg ccatttcatc gatatatgaa
     3181 atcacttcat cgatcgtctt gcatctatga ttcaacggat tcacacgaaa gccccagtcc
     3241 tttgcaagtt gcaaagcctt gtaatgatcg gagacgattt tcctgtccat gacgtaataa
     3301 acaaagacat ccagattcct ttttctgaca atttgcgggt taagctggcg gacactgccg
     3361 gcagcggcat ttctgggatt gcggaatggc tcttcgttgt tcgcaagctt ctgttcatta
     3421 agtctgttga atgatttagt aggcataaaa atttctccgc gcacctcgac gggggccaga
     3481 tatggtattt tcaatggaac ggagtttatg gtcttgacat tctccgttat atcctctcct
     3541 gtaacgccgt ctcctctggt tgcggctcta acaaggtatc cgttatcgta ctgcaaagat
     3601 acactgagcc cgtcgatctt gagttctgcc gtgtaagaat agtctttagt ctccttggcg
     3661 attctttgat cgaagtcaat cagttcttcc ttggaaaaaa catcggccag agataacatc
     3721 ggccgttcat ggataacttt tacaaagcga tcctgaatgc cgccaccgac tctttgcgaa
     3781 ggagaatcct tggtcaccag ttccgggtgc tcttcctcta accgaaggag ttcattcatc
     3841 aatgtatcgt attcgctgtc cggcacgctt gggttgtcca tgacataata ttcgtaatta
     3901 tatttattca aagtatctct cagaaatttc aatctttcca taactgtcat gttcttcacc
     3961 tgactttctt gatgcttgga tgatcgctca tcagaatctt gattccggtt tcgtgaccga
     4021 aagcgatggt cgcccggttg tcacttaaag caacgacaac tccgattccg aatgtcttgt
     4081 gctcgatctt gtcgccgatc gaaagcttgt tgtcaaccga ttcgtattcc ttgctttcta
     4141 ccgccggtag cacatgtttt ctttgcgact gtattctttc aagcttgatt tccctcggct
     4201 tggaaagtcc ggaatgctcg atatattcct tgccgatctc accgatgaat tgagaatccg
     4261 gattgctggc aaggtgtccg tagatgaaac gttctctggc gttggtcaga aataccattt
     4321 cctttgctct agtaagggcg acataaaaca gtcttcgttc ttcttcgaca ttcgctcttt
     4381 cctgtaaact ttgtccggac gggaaaagcg cctgttccag acaagcgacg aagaccacct
     4441 tgaattccaa acccttggct gaatgtaccg tcatcagact gacgtactca tcgtctgtca
     4501 atttgtcttc tgcttcttcg cgcaaggcga gatcggtaag aatttccaat agtttttcgt
     4561 agttagtgac atcgcggtcg aattccttga tcttttccaa aatgattgtt ttgaattcat
     4621 tcagattatc gatccggcgt tcggcttcga ttttacgatc agcagccaaa tcttcatttt
     4681 gaagcatttc cagataaccg cttttttcca gtaatctgtc atatgtgtca agcagattta
     4741 tctcttctat gttccggcgt atttcctgca gattggcttt cagttctctg aggcggatga
     4801 gccccttgat tccgataccc ggaatatcat catcgatcgc ttctgtcagt ttgacatttt
     4861 ctttcgtcgc atactctttg attttgtcga aagacgtggg tcctaccccg cgtttcggtt
     4921 cattgtatat ccgttcgaag gcatagttgt ctcccatgtc gataagcaat ctgagataag
     4981 cgattacgtc tttgatttcc ttgcgtttat aaaaagaaat atttccgatg actttatatg
     5041 gaatcccgta ccgcaggaac atgtcttcga atctcctgct catcgcgtta ttgcgataaa
     5101 gaacggcaaa atccctgtag ttatatcctg ttcttgccag ggtttttatt ttgttatata
     5161 tgtaatatgc ttcttcctcg tccgtctcgg ctttgaaatg ctggaccttt tctccctcgc
     5221 caagctcgct gaacagttgt tttggtattc ggttgtgatt attgtcgatg accgaattgg
     5281 ccgcttccag gatattggtt tttgaccggt agttttgatt caggattacc ttatggtatt
     5341 caggaaaatc cttcatgaac ttccggatgt tttcgatatt cgatcctctg aaagaataaa
     5401 tgctttgatc ttcgtctccg acgatgaaca gattgtgatg cgttttcgct agattataaa
     5461 cgatctcgta ttgcaagtta ttagtatcct gaaattcgtc caccaggata tattggaaac
     5521 gattctgata cttttccaaa acttcaggaa atttagtgaa aagttccaaa atcaaaacca
     5581 aaagatcctg aaaatcgacc aggttgtttt tcttcaggta tttctggtaa gccggatata
     5641 taacttccat aaccgatttg atcggttccg gcaagaattc caattgttcc ggattnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatacc gatctcctcg atcaggtaag
     5821 caatccgatt ggtaagaacc ctcgtttttc ccgaacccgc tccggctacc gccatgatcg
     5881 gtccggaaac cttgcgaacg gcttccgctt gttgcggatt catttgttca ataatagcat
     5941 ttttcaaaac gatcacctac taatattata ccatattata agcggttttt gtcatgtctt
     6001 ggccacgaaa aaaccggttg tttccagccg gtttatttgt tttctttgtt aatcttgatg
     6061 tctatcaaaa gtcttatcaa tcgcttgata aaagctgctg tcaccagtat ggccatgagg
     6121 gcgaatacga tggtgaatgt gattaccgta ccgctggaat cgcctttgat tgccataatc
     6181 aggttgatga ttgtcccgaa ccatccgata atggcaaata tcaaatacag caggagatat
     6241 actattttgt ttttcatcag cggaaactcg acttcagttc gacgatccgg ttgaaaacca
     6301 gattgtctga ttttgattca tagtcggcag cgtaatagcc gttgcggatg aattggaatt
     6361 tttctcccgg gacggagttt ttaagatctg gttcgacaaa tccttttttt gtttgccatg
     6421 atgtcggatt taatctttct tttaacggcc tgtccccatc agaatcaatg agcaaaggct
     6481 cgaataaatt gaaggttgca ggaattgcgg tagtagcttc gacataatga atgttgccgt
     6541 tgggctttct ttcattgaat ccggagttcg atttggtctt ttcgtcgtat gtgcaacgca
     6601 attctttaat ctcgcctttt tcgtcataaa cgatatcttc gcatttgatg aagtacgcct
     6661 gtaacaatct tacttccact cccacggaaa gccgtttcca atgtttgttg ggtttttccg
     6721 gtacaaagtc ggatttttcg atgtagatgg tttttccaaa gggcaatttg cggctgccta
     6781 acccttcgtt gtccacattg tttgtgacat ccaacatttc cgtttcattg tttggataat
     6841 tggtgatcgt cactttgagc ggatccaaaa cggcatgcat tcgtttggtc ttgtcgacga
     6901 gatcggccct taaagtgttt tccagcattt ccgacgaaac ggtggagttt atacgggaaa
     6961 gaccggtgct cagaatgaaa ctgacgatcg cgtcttttgt aaaccctcgc cgcctcaaac
     7021 cggcgagtgt cggcatccgc ggatcatcgt agccattgac atatttttct tccaccaatt
     7081 gtctcagata tcttttcgac attatcgtat tggtgatgtt gagccggccg aattcgtatt
     7141 gatggggttt gtgtggcgtc tcgcagttat cgatgaacca atcatacaaa acccgatgat
     7201 ccacgtattc aagcgaacaa agcgaatggg tgactccttc gatcgcgtcc tgcaacggat
     7261 gagcgaaatc atacatcgga taaatacacc atttatcgcc ggtattatga tgcgtcgcat
     7321 gcagaatccg gtatataacc gggtcgcgca tattgatgtt cgggctggcc atatcgattt
     7381 tggcccgtag cgttttttct ccgtcttggt attttccttg cctcatttct tcgaaaagac
     7441 gcaggttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt tttctccgtc
     7561 ttggtatttt ccttgcctca tttcttcgaa aagacgcagg ttctcttcca cggaacggtt
     7621 gcgattaggc gattcctttc ccggttcggt cagggttccg cgataggcgc tcatttcctc
     7681 ctgggacaaa tcgcacacat aagcgagacc tttcttgatc agttttatcg catattcata
     7741 tgtcttttca aaataatcgg aaccaaagaa aatattggcc ggttcatatc ccagccaccg
     7801 aatgtcttct ttgatcgctt ctacaaacga agcgtcttca ttgatgggat ttgtatcatc
     7861 gaatctcaag tttgtatatc cgccgaattg gcgagcgagt tcaaaattgg tcacgattgc
     7921 ccttgcgtgc ccgatatgaa gatatgcgtt cggttccggt ggaaatctcg ttatgatttc
     7981 ctttatcttt ccgctctgca aatcttcctc catgatggtt ttgatgaagt tggattctat
     8041 ttccataagt cattcctcct ctacaagatc cagacgcgaa tatccgttca gcatgtaact
     8101 gtcggctttt cctgtttttc ggtatttgaa gtagcccgcc acggcgatca tcgccgcgtt
     8161 gtcggtacaa tatctaatgg ccggaaaaaa taccggaacg ccagtgatct cttgcgtaat
     8221 tttggccctg agtcctctgt ttgccgccac gcctccggcg acaattactt gctttacatt
     8281 gtgttggttt gcggcctctt ttgttttttt gaccagaaca tccgtgacag cttcctggaa
     8341 gctccggcaa aagtcggcaa cctcatacgg ttctttcctt tgttccagat tatgatgcag
     8401 gttgatgaca tgggatttca aaccggaaaa actgaaattg aaatccccag aatcgatcat
     8461 cggccgggga aaaccataga tgtcttttcc tgttgcggcc agtttgtcta cctgcgggcc
     8521 gccgggataa ggcaagccga gaactctggc caccttgtcg taagcttcgc ccacggcgtc
     8581 atccatcgtt tctccgagtt tttcgaattc ataatgttct ttcatcaaaa caagttccgt
     8641 gtgtccgccg gatacgacta aggccagcaa aggaaactcc atttcgtgtt cgatgccatt
     8701 ggcatatatg tgtccggcga tatgctggat cccaaccaaa gggatgtcat aattgagact
     8761 gacgacttta gcggcattga tgcctatcaa caatgatccg atcaatcctg gcccgtttgt
     8821 tacagccacc aaatcgatgt ccttataact ttttccggct ttttgcattg cttggtcaaa
     8881 caccatggtt atgcctttga catgctccct tgaagctatc tcgggaacaa cgcctccata
     8941 agcctgatga atatctatct ggctcagcac ttcgtttgac aatacggttt ttccgttttc
     9001 gaccagactg acgcttgttt cgtcacaact gctctcgacg cccattacaa tcattgtact
     9061 ttcacttcgc atttcttaag ataaagcaag gcgtcttcgc cgttgtcata ataattgcgg
     9121 cggatggcaa cttgcctgaa tccgaatttt ccatataggt ttctagccct gaaattggaa
     9181 ggtctgactt ccagggtgat gttttccact tgatattccg acaaataatc aatgcagaat
     9241 cgcatgatat cgtcacccaa accatggtgc tggaactccg gcaaaacata tagattcagt
     9301 atctgcgcat tcttgagatc gatccagatg ctgacatgcc cggccagttt cccggtcgtt
     9361 tcttcccgca ttacgaaata atgcgcgaaa ggattgatat ccagttcgtt tgctagtgtt
     9421 tgttccccta aagtatgtcc gagaaccaaa cggtcagctt tgatgatttc cggaaggtct
     9481 ccgtgagtca tcggagatat ggcgaaccct ttcattgttg gatgtttctc tccgcttccg
     9541 tcatttgtaa ataattcggc acaagttcgt ggatgttttc gatagatata agcaacccgc
     9601 tagtaatgat tttttcgata ttgtatttcc cgctatccac aacttcgtaa tccatcggtt
     9661 tcatgccgtc aaggaattct tccttattgg caagacaatc tggtttcata tattccaatt
     9721 ttccgttgtt ttgtttaaaa gcggcaataa atgcgttttg tcttctggca tcgatcagcg
     9781 gaacgatgat cttggcttcc gatccggaag ccagcaaagc caaagatgac acagcgctga
     9841 cttttatgtg attgagataa ccaatcattt tggctatcgt gacaccaatc ctgacaccgg
     9901 tgtaacttcc cggaccgatt ccgactatta tctcatcaag ttcgttgagt ttccatccgt
     9961 ttctttccaa cagttccgcc ataagcggaa ttgttgttac ggagtgatcg tgatttcccg
    10021 atttgattat attttctttc tccctgttgt tttctaacag agaaagatac acataatcag
    10081 ttgcggtatc gatcgccaat cgtttccacg actttggcat aagcgccaca tccttcgata
    10141 gttatttccc gggagctttc cccgctgatg acaatgtcca ttttcaagaa ttcttttggc
    10201 aaaatgccat ttgcaaactg gtaccattca atcactgcga ccccgtcccc gtaaatgaat
    10261 tcttccagtt catatccgtc tccgccttgt tccagccggt agacatccat atgataaagc
    10321 ggcaagttcc ccgaataggt tttaagaagc gtgaatgtcg gcgaattgac attgctggtt
    10381 atccccatgt gcttggcaag atacttggtg aacgtcgtct tgccgctgcc gagatcgcct
    10441 gaaagtccga tcacatatcc ggggtgcaac gtgccggaaa tgaccttggc cagcttgtcc
    10501 atatccgcaa gcgacaatac tattatttgc agtttgatag cggccacgct cctttcctta
    10561 tactactgta agattatacc ataattttgt gtaatggcat attattctca tgataaacca
    10621 ccattatgat tacctggtgt atctttattt ttttcttaac gaacggcgtt gtgtgttata
    10681 atgttgtcaa ggagtatggc aaattcaaat ctttagaatc cgttgtcata aaaaacgact
    10741 atgtttttga taattcacaa tccgctttcc agcaacatga aatctaaaat caaaacattc
    10801 atgatggtcc gatttttcaa gaaaaaccac attccattca atttgcgttc cactttgaaa
    10861 atcgacaatc tcaacgaata tctcgataac aatccctcca tcaccgacgt tttgtttctg
    10921 ggcggcgacg gttcgataaa ccatctcatc aataatgtgg atgtcacaaa aatcaaacag
    10981 accatctatt taggaaaaag cggatccgga aacgactttc tgagaagtct aaaaaaaatc
    11041 ggatccggta atatcaccat cggcgaagcc gtcacagaca aaggttcgac caagttcatc
    11101 aatggagtcg gcgccgggat cgacgccacg gtctgttatt atgtcaatcg ggacaagaag
    11161 aaaaaaagaa tgtcctattt caaaaacgtc ttcagtgcca tgaaagcttt cagtccttcg
    11221 gctgtggata tatccatcga tggaaaagaa tatcattatg acaaatgcta ttttgcggct
    11281 atccagaacg gagagtattt cggcggcggc atgagagtgg ctccgaacgc ggacatattg
    11341 tctgatgagt atcgcatatg tgtcgctcat tccatgagca aatctatgct tcaattccta
    11401 ttgatgacga tttattccgg actccatgtc catttcaaaa aatgggtcac tttgtttacc
    11461 ggcaaggata taactgtaaa atttgcggac aagcgttatt ttcaggctga aggcgaagtc
    11521 atggttgatg tatcggagtt ctctgttaaa aaaaatatgt caaaggagtt tcacgccttc
    11581 gacaaacgta aaataaaaaa gtctctcgtt aaataaattt acttattcac tcaaaataaa
    11641 agtctgattg tttttatccc caatcagact ttttttatta ttttatgttt ccgggtttac
    11701 caagtaagtt gaacatgttt ttcttatatg cttcgacccc tggttgattg aacggattaa
    11761 ctcccaaaac atatccgctg atagcgcacg ataattcgaa gaagtaaacc aaatatccaa
    11821 aagtgaaaga tgacaaattc tttatcttcg ccacaataca aggcacttct ccggaagaat
    11881 gggccattaa ggttccttct gcggcttttt tgtttacata gtcaaccgtc ttgccggcaa
    11941 gataattgag ctgatcaaga ttttccttgt cttctttgat aacatgatcc agattcggtt
    12001 cttctaccaa aaggaccgtc tcgaacaaaa gtttgcgtcc ttcctggatg tattgtccca
    12061 atgaatgaag atcggttgta aaattggcgc tggccacaaa cagtccttcg ccgttctttc
    12121 cttccgattc gccgaaaagc tgtttccacc attcggcgag actggccagt ttcggttcgt
    12181 aggaaaccaa catttccact gatttgccgc tcctgtatag ccagtttctg atcgccacat
    12241 acaagtgaac gtcattggcg aacacatctg cttttttgta ttcctttttg gccgccttgg
    12301 cgcctttcat tatttcggcg attttgattc cggcgcaagc taacgggaaa agtccgaccg
    12361 gggtcagaac actgtagcgg cctccgatat ttgatggcac gacaaaactt tcgtagccct
    12421 ctttgctggc catttttctc agggcgccct tttccttgtc ggttgtgaca acgatccttt
    12481 ccttggcgcc atcaattccg tattttgtct cgagaagttc tttcaaagcc cggaaagcga
    12541 tagcggtttc cgtagtggta cccgatttgg aaatcacatt gacggcgaaa ttgcgatctt
    12601 tgagatatat gagcaaatcg gaaatgtatt ctgaagacag gttttgtccg gcaaagatta
    12661 tctccagtct tttacggttt tcaaaataag gtttcaacat atccaaggcg gctctggtgc
    12721 cgagataaga ccctccgatg ccgataacaa ccaagatttt tgccatcttg cgaatctttt
    12781 tggccaccag ttgtattctg gcgatttctt ctttgtcctg attaaccgga agatccagcc
    12841 agccgagaaa atcgtttccg gcgccggttt tttgttctaa tgtctttctg gcagcggtga
    12901 tttctttttg cagggatttt atctctgtat cggaaacgaa agccgataag taacttctgt
    12961 caagttcaag gattttttcc atattcttac ctccaaacga atcaaatgtt attatatcat
    13021 agcaaacccg cgatggaaag atttagaaga cgaataaaag cggctttcat tcgccgccct
    13081 tttgatattt ggcaatccac tccggtaacg ttttttcaaa aggtttgaat ccggtcgaaa
    13141 gctggatgtc cacgtatttt ccgcgtttgt gtttgcgcgt gcttttgtaa ctgagactca
    13201 atttgtttcc ctcgtttatt ttcagaacct gtacttcgat ttcatcgccc gtttttagga
    13261 aatcttcaat gtttctcaca aaaccgtcgg tgatttccga tatatgaacg agcccgtcgt
    13321 attggtcatc gatcttgaca aaagcgccgt atggcttgat cccggtgact ttgcctctga
    13381 cgatgtctcc ttctttcata cacaacacct cttgaaatta ccaaacaagt ataacatatt
    13441 attgtgatgc ttgcaaaaga aaagacatgg ttcaacccat gtcatatgaa tgaatcactt
    13501 ggcgttgttg tgcgccaccg aactggctgc cgccgccgca atagcaccga tgatgtcgtc
    13561 aagaaaagtg tggcatttgt cggtttcttt tccctcgcgg tcgagcttac cgatgattcc
    13621 cggttttgtc ttgtcgacat aaccgaagtt ggtgagtccg atcgaaccgt agagattgca
    13681 gatggaaaga acaagaatct cgtcgattcc gaatagcgga ttgtcatcgt tgacaagcga
    13741 ctccaacggc tctgacagtt ttccctgctc ggccagcttg tcgagttcga ctccggtaag
    13801 aacagcgttt tggacttccc ttttggcaag cacccggtaa acggcatcct cgcaaacctg
    13861 tcggttcaga ttttctatat acggggcctg caggtcataa acaatatccg tgatgtcgga
    13921 tatcttcacg cctctttcct gcaataaact gatgcagtgg tcgtggatcg tcttggaatc
    13981 tggttccatg ccaatctatc ctttcttcaa acggtggctt cgacgccgat gacgccgggc
    14041 accctttcaa gcaatgtgtc ttcgaccagt tctttcaggg tgtcttcgaa tccggcgcat
    14101 ccgacacagg ctccggtaag cctgacatag actatgccat cctcgaattt aatatattcg
    14161 atatcgccgc cgtccctgtt gatatagggt ctgatcacat tgatcacttc ctggacttcg
    14221 gcgattattt cttcgtaagt catgttatcc tcttcttttc tcgtttattc gtcccgtttt
    14281 ttcttggggt cgtaattggg attttttaaa ataaaataag cgcagttcat cggacaagat
    14341 tccgcaagat aatcgggaat cgtcctgtaa ctgttggttt tttcttcgag agcgaatcct
    14401 tttaaacgta aaatgccggc cgaataatcg ccgacgacat acggataaat atcgaaagcg
    14461 tcggtatagc gcttgttgaa atcatccatt ataaaggcgt cccggtggtt tttaatcatt
    14521 tcgaaatttc cgtgttcggt ttcaatcatg ccctatccct ccagtatttt attttaccac
    14581 attctcaaaa aatatgaagt aaaaacactt ggcaaagaaa aaaggccgaa gcctttttca
    14641 taacatcatg aatacttcgt tttccaacaa cacgacggct tcgcagatta tggccttctc
    14701 ttcgccaacc ggcccgatgc cttcataggt ggtcgccttg atggaaatcc gctccacctg
    14761 cacttccatg attctggcga tgttggctcg catgtcgtcg atcaaaggac gcaatttggg
    14821 attttccagg gaaatcatgc aatcgagatt gatgacttcg tatcctttct ttttgatgta
    14881 gtcgtatact tccttcaaaa taaggccgga gtcatatcct ttatacaccg gattatcgct
    14941 aggaaaaaat ttgccaagat ctccgagggc cagagctccc agaagcgatt cggcgatcgc
    15001 atgcatcagg cagtcggcgt ccgaatggcc gacggctccc ttatgatagt cgagtttgat
    15061 gccgccaaga atgaagggct tcccttcttc gagcgggtgg atatctttgg aaaaaccgat
    15121 tcgtatcata tcaatgcctc caatatcttg atatcttcaa aggttgttgc cttgatgttt
    15181 tttagctctc cttcgacaag ccagatgggt agtcccgttt cgttctggta aacggaaaca
    15241 tcgtcggtat acgttcggtt gttgtcacgc gccttgcgat gggcatccat tatcctgtcg
    15301 gtcggaaatg cttgtggtgt ctgaagcctt ttcaccgatt cgcggtcgat atcgccaaaa
    15361 acaaagttat cacgtaccag tttaagggtg tcgctcgccg atatggccaa agccgcggcg
    15421 gaatggaaac ggagtccttc ttttaatctt tccaaagctt gacggctgag attcggccgc
    15481 gcgccgtcat ggatcatgac gacatcttcc ttgacaagcc tcaggccggc gagtacgctt
    15541 tcctgacgcg tatcgccgcc gacggcgact ctgattttct ttggcaacac attcagaaac
    15601 tcttcttttt catcattgtt cacgaccaca atgatttccg agcattccgg atcgcccaaa
    15661 aatctttcca cactgtaaag gatcatcggc ttgcctttca ggggatgcag gtttttgtta
    15721 tggcccagac cgctgcgcac gctttggccg gctgccatta tcaaggccgt atacataaaa
    15781 tcactctccg aagacggaat agttcttctt ggggttgtga atggcttccg ccaaagactt
    15841 gttggcaatt cccgtcaatt cgttgattgt gtatgtatcc caaggttctc tttttatgat
    15901 tcccagattg ggatatacaa cttgttggat accgccgatg gatagtgtca ggttgacaag
    15961 atcgccgccg ctttgaagcg cgcggtgaag tacttcgtat tttttctgat ccttgattat
    16021 tatggcaaac tggattccgg ttatccggaa tatgctgtta acatctttag caaaattgaa
    16081 tcgcattttt ttgatgtatt ccgcaatcat cagattgcca acatcacgtc caaaccggtt
    16141 gttgatatcg ggaatgttgg tgagttggat cattatgaga tagaatggat cggtttcctt
    16201 catcgcctga gtcagatatt ggatcaggtt ttgttccgaa ggaaggctgt cgatttcgtg
    16261 gattgacgta tccggaaaca gtttgatatc gatcggtttc acagtggaaa tgatttgggt
    16321 cttccggtca aactcgaacg cttttccttt ttcttcgatc caaatcgaaa cgttttctcg
    16381 gacgatccga tacttcatat cataagtcgg gtttttcttg ctgacttttt ttatggtcga
    16441 cagataagcg gcccggtcgt ctttgtgaac gatatcggca tattcttcca ttgccagatc
    16501 gttttttcct ttcccgatca gtagcgatag ttgatcggtg atgaaaagac cggtcagatc
    16561 gtcatcatag aaaatcaaac cttcgctgat aaggccgatc gtggaaatat acctgagacg
    16621 caacaaatcg gaagtcgttt tgatttcatc gagttccttt tcgagttcga aataatcata
    16681 gtcggtcaaa ttcttggtta tcctgatctt tcgtttgaat gtaaacgcat aaattgccac
    16741 catcaggtaa agtgtgtatt cgtaatatgg ttttatgaca tcaaaggaaa caaacggtac
    16801 aagctccgga aagaagcgca tgatgataaa agcccctccg gaaataaaga agagaatggt
    16861 tttccacacg ctaaatttgt gataggccaa ggcgctcgta accatcaaaa gaaacatcgc
    16921 gccaagggat atgtaaatga acaaataggc catgatcagt gcctctttct catgaattta
    16981 tttcttgatg ttggtgctga catagatgat cggataataa taagttagaa aaatccccag
    17041 gaataccaga ttgtaccaag ctgagacgta acgatcgccg gaaacatgga cgcccccaag
    17101 aatcaacagg aaaatcaatg cccagacaaa tgtcatcccg ttgttgacgg ttttgaagaa
    17161 ttttgtttcc gcatagtctg cccggtaatc atatttacgg aaatcgtgga caatcggctg
    17221 gttgattatc caggatatga agaacattcc ccccatcagc cccagcaaca tgtcgtcgtt
    17281 atgcaaaaca ttgaacggtt gccagaatat cgccataaac aacatcgcca agtaaattcc
    17341 ggcccatgaa aattcaaacc aggatattct acggaaaaac cgccatttca aatatcccat
    17401 ggctagcgat attccgagag cgaacggcac aagccagatc atatcgatga aattacatag
    17461 aaaagcaagt gcggaaaatg ccagcagata tacgaacatc aattttgctc cagcgttttt
    17521 cactggcagt ttgggctgga atttgacttc tgttttctta caatcgtcca gctcgaacgc
    17581 atcgacaacc ttatagagaa gttcggatga tcccgtgtac ttcagaccgc cggattccat
    17641 gacgttttca aaacagactt ttttcagtag taaattagac aacttatcgt ttgttgtata
    17701 tattgtcaaa tccggatggg gatattcccc tctgtgaata gtcaggtttt tatccttgat
    17761 gcggacataa taactgctct ttccgatttg gaaattgata tggttttcca atttgccgag
    17821 agtcttgcgt ccatagttgt gtttgagcat gaccatgatt tcgtcgttgg tgagataata
    17881 gaaagaatcg tcgttttctc cgtaatacag actttcttcg attttgtctc ccaaaacaac
    17941 gccggtatgg aaccgtccga acagtccggc ttctggacga acccagctgc ccgtcaaatg
    18001 gagattgtcg aaaaacatca ggtgttcgga tgcttcgatc gctatctcct cgtttatcgt
    18061 tagccctctt ctgacctcgt ccagactgac catggcggat agttcgcgcg ggtttccttg
    18121 tttgatcccg actatggttt ttttcaattt cgggaatttg ctgtagatct gcgccaatat
    18181 ttcttgttcg ccgatttcgg tattttcatc ataaacgtaa tcgacacaca gagcaccttt
    18241 cttggccccc gtagatgtcg gttcatacag actgtaattg aacagcctgg taatatatgg
    18301 ttttgccagc ccggcacagg aaaagcagta attcatttct gttatgccga tgttgtgcgg
    18361 cttttgattc aaagcaagat aaaccgttct tatccgggtt ggggattcaa gtcccggatg
    18421 aaacttgtcg atgagttcga gatgttccgt caaatcggga aaatacttct tataaaatcg
    18481 atccggcttg gatccgacaa tgatatgttt cgcggctatt tccttgtttg tggaatctat
    18541 gacatgtgtt atccggccgg ttacatctcc gacatattcc ttgatccgcc tgtgttggat
    18601 aattttcgga ttgatgattt taagcttctc catcaacagt ttcttgattc catccaccga
    18661 gaccggcaaa taataaaagc cttctttaag accgagaaaa taaccagaaa aaaaattgta
    18721 gctgctgaca tcgtcggaat ttagtccatt caactgacaa ttcatcatga actcgtttgt
    18781 gatgagcgga tcggaaaaat attttccgag caaagcggag aggctgtaat cgccccattc
    18841 gatcatcagg gatgtcaaag tataatcggt attcttaagc atgtattcct gttgcatgac
    18901 atagttttcg taatgttttt cgagatctcg gaaaaaccga tgaatttcat ctctttgttt
    18961 cggataataa cgcacgagat aaaccagaaa acgttcgaaa atattgattc tttttcgaaa
    19021 ctcgccttcg gttgtttcca ccatatcgta ttcagatact tccccggtca aatcagtatc
    19081 aatgccgatc gcgtgtaaat atttttccaa caatccattt tggttcaatc cagaaacaaa
    19141 tgccccggaa tcgtaggcaa aacgatattt gttgttttcc ggatccgtga tctcgacttc
    19201 gctgtcgcca tccgggtttt cctttccatc gatgatgacg gaaacgcttc gcattttgcg
    19261 cgcaaggaaa agcgcgctga ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnntcgatga tgacggaaac gcttcgcatt ttgcgcgcaa ggaaaagcgc gctgaccaat
    19441 gattcgatgt cagaaccgat taccagtacg tcatactgtt ccacgagcgt cacctcccgg
    19501 attttccgtc ttctcaaatt atatcatata ggatgttaaa attccacgtg taaaaagaaa
    19561 agtgccgaag cactttcaat tgattttcaa gtatttttca aagtagagac taagcgtttc
    19621 ccgactgaca ttatagtcga gttgacgatt tatcgcgaca ctcacgtgtc ctgtttcttc
    19681 cgacaccacg atggtgagag cgtcgctttg ctcgcttata ccgatcgcgg cccggtgcct
    19741 ggttccgaaa tgcaacggca ggtcggttct ttcggtcgaa ggataatatg cttttgcgca
    19801 aagaatctga ttgtcgcgga tgatcaccgc tccatcatgt agcggggtcg tagtcacaaa
    19861 aatagatgcc agcaattcag aagaaaccgg agcgttgatc atcagggctt tgcttatgaa
    19921 tgcggacaag tcaatgcttc tttcgatcgt tatgagcgct ccaaccttac ggttcgacaa
    19981 aaagtcgacg gtgtccataa gtttttctgc caattcgttg ttgatgatcg ggttacggct
    20041 ttttttatgc agcattccga tctttttgat tgtcgaacgt aattcattga tataaaccat
    20101 gacaatcatg taaggaatcg gaatcattgt gaagatgccg acgaaaagtt ccgttccgga
    20161 ctggttccct ttgacaagaa gaaaaatatc caagacaagc aacaaaacca gaaaaatctt
    20221 ggccggacgg accgtcttat gcgccacctt cgttctcaac agtagcaaca atgcggcata
    20281 tagatacgta acaatcgttc ccaaaataaa cagatccact tgccgtcccc ctctcgatcg
    20341 ttattcgcat atattatttt atctcaaatc aaggccatta taaagccccg tcgtgttaat
    20401 ttgtggtttt ttttcgatcg gtcttgtgaa agacgtaatc cagagcgaaa taagcgatcg
    20461 gtatcagaag cgccgaagcc aggaacgggg ctatgatgct gtaaagactg ttttgatcat
    20521 aactcagtcc gatccagtcc tggataatga aatcaaatcc cgttgtcaac aggtcgctta
    20581 tcagcaatct ggcaagaaaa accgtcatga cagctgtgaa ttgcattgct gtcttgttct
    20641 tcggtttgaa tgtgaagaat gcattcgcga aataagaaaa tatcgatgcc gaaacgaacg
    20701 ctatcgtgtt cgagatgaaa gacgaccagc ccgtggataa cacagggatc agaactttgt
    20761 tgtaaaaagc ataataaact gacatatgaa tcgcggtatt tattacgccg accaaacaaa
    20821 atatcagaaa tttcttagtc agaaaattct ttctgacaaa caaccatagt tttttcaaac
    20881 cttcattcct ccgatgtttc ctcgatcaga taaatcgggc gttccaacac ttgccttctg
    20941 atctgataaa gtaagtaaat gatcccgtat gtcacaaatc ccaaagcgaa gacggcaatg
    21001 aaaacgaaaa gcccgatgac gaattccgct aacgtaaaca atccgaatcc gaacaaaagc
    21061 gcggtcgcta tgagaatggc tactgaaagg aaccatacaa acgccggcac aaccatcatc
    21121 aatgtcgaga attggttcat gccgtcaaac gcatagcgaa acaacttttt gaaactccat
    21181 gtgcttttgc cgtattttct gggaatgaat tcatattcca aacatttgcg tttatatccg
    21241 acccaggaaa aaattccttt tacaaaacgg taccggtctt tgatcgccaa aaaagcttcg
    21301 accaccttgc aatccaaaag ctgataatcc ttggcaccgt tgtcaagcgg catttccgtg
    21361 tgccggttat aaatacggta aaaacaattg gcaaaaaatg tcctcagttt cggttcgcct
    21421 tttcttgttt tacctttggt atagacaatc tcataccctt cttcgtaata ttcaagcatt
    21481 tccggtatca aggctggtgg ttgctgtaaa tcgcaatcaa tgatgattac ggcatcggct
    21541 tctcttgaat gttccaatcc ggcgtgcatg gccgcttcct tgccgaaatt acgggagaaa
    21601 ctgatatatc tcaccctttg gtcggtagac gccagtttct tgatttcatt cagggtattg
    21661 tccttcgatc catcatttat atacatgata tgaaactcgt atcccggacg ctcgagaaat
    21721 cctgtaatct catcataaaa cggtctaaca ttcttttctt cgttgaatgc cggcacgatg
    21781 atattgatct ttttcacgat tcattcatct ccttggatat ctgttcttta tttctttttt
    21841 tcttttcgct tatccatccc ggaaggaaaa ttccgagata aacggcaata aacgccaaac
    21901 tgcccagcgc tccttctttc agtcctttgg gaatgaacct cagttctatc gacacatctt
    21961 cgatgcctgc aggcactatg attcccagca ttcctccgga tgcgcttatt gtttcatatt
    22021 gttgttcgga agtcactgtc cattcttccg aataagtcac gggtatcacc acgatttgat
    22081 cgtaagtcgt ttcactagtg cgaagatagg acaaagaaat cgtgccgttt gtgatctcaa
    22141 gcgaaccgtt ggcgtagagc ttattgccat tggtcagatt ttcatattct tccagataat
    22201 ctccatcata aacataatgc aaggaaaact ggaaaaaatt gtttacgttg gagtacatgt
    22261 tgccggtttt gaatatatat acccgaaccg gggcagaatc aaaataataa tagtctttga
    22321 atccgtaggt ttcgttgcct tcggcatcca cgaaaattac agtccccagt ttttgactca
    22381 ttctaagata caacatgccg gccccgtctg tgtattcgat acccgacaga tcgaaaacta
    22441 aataggcgct gctgtccctg gccatttcca tcctgtacgt tatcgacaaa cgggtattaa
    22501 gtccgtttgt ctcaaaataa atagcaacgg gttccgatcc gaattcacac tttacgtcgt
    22561 aataatcgga agccggaaat tggcttttat acattgagcc gtcggcaaat tccatataca
    22621 cgtctccgga ataaatcccg ctgttaacat tgatataaag cgcccccgca tcaaacccga
    22681 tattcagctg gctgccgtca aaccggaaaa acaaaccgga agggttgtaa tcgtcgccgg
    22741 atccttcaaa agccacaagt tcgtacgaag taatggtctt ggagggatat atcgtatttt
    22801 cactggaacg gtcatctaac attatttctt ccagattgaa agtcatgtcc tcgtatcttt
    22861 cggtatcgat caatgcggcc atcaaaaaaa tcttttgcgc ttccagattg ttgttgaaat
    22921 cacaaaagcc ttcatattcc gaataggaag cataggattc gtatacttgg aaattttccg
    22981 cattcacaat ccggaacaat acatactcag aatctgaata tatcaattca aagtaatcac
    23041 tgtcaagata atacgcatgt tcggtacttg taagcacgta tttatatcca aggaactgat
    23101 ttaagaaaag cgccatggta ttcaatttgg atttactttg gtgctcttcc gcattcgtac
    23161 tcagattaaa aagtaaagtt gctagcgtat ctgattcata gtccgtccag gaatggaata
    23221 tttctgtatt ggtcggataa acagtcatcc ggttgaaatt agtgtcttcg acattgaaag
    23281 cctgcatttc cacataaacc cgataaaacg attccccgtc ctcggcaagt tgttctttca
    23341 aaacatcatt gatcgaatac atctcttcga acgtgtcgtt cttgtttcct atgaaaaaag
    23401 cgttcccgta aatgtttgcc agcgcgacac agcattccgc aacaaacaag cattttaacc
    23461 atccgggttt cttcatccag ccaaatatta ggaacagcag aatcacgaca gcgctgacaa
    23521 tcatcatcgc ccaatcaagc gtgagaacat ccgcaaactt gaaatccctg agtagatcga
    23581 gtttgtggat gtagtaatac accaaataga tatccaaacc cataagcaag acggccggga
    23641 ttgtcagttg tttcatattc agtttttcaa atccagtttt gtcgaacaca tgtgcgagaa
    23701 tgattgtcat aactagcggg taacaattga tccatcttgt atagggctga tccgtcgttc
    23761 cggaaaataa ataagagaag aacggaaaaa tcgccatcag cacgccgata aggatagtcg
    23821 ctttatatac cttgcttgac cggtctttca aaaagaatat gccactcatc attaataggc
    23881 cggtcattgt gatatacaga gatatgtgtt cttttgtata gtcctgagaa aaaccataaa
    23941 atcccgtcgg gtactgttcg gcaaacattt ttgccagaaa acggatatat atttccggtt
    24001 caaacaattt cagttcgtag cttccgatct gaatgcccca gtaattgaat gtccctgttt
    24061 gcctgtaggt gtcctgtaac acatacaaaa tggaaggata cagaatgatt aggctcattc
    24121 cgacccccaa caaaagcaat ccgaggaaaa tcgccccgtc acaaaaaaac ttcccaaacc
    24181 ggaaatcctc acgtctgaca tattccatga taaacatcaa gcccgtgaaa atcaaagcca
    24241 tgtatgcaag atagaaatta taaaaaacca gcacgaaagc gaataacgga acaatccatt
    24301 tttttccttt aaacgaccag tggatgacta tgagcgccaa cggcagataa aatgttaagg
    24361 atagaaatgc cgggaacgcc atgaaactga cggttcctcc gctgacaaaa tatacaagac
    24421 ccatccagaa tattgtctta ttcttcatcc ctttaagatt caggtaataa gcaaaaacca
    24481 tgactcccgc ccatatcttt atcaattcgg ttaccgaata ggcaagctga gtgggcatca
    24541 gataactgag aataaaagtt attgtggtga aaatatcaag cgggatgtaa taaacgtcgg
    24601 aataatacga agcccccaaa taattgttca gattgaaaaa cgatatcgtt ccggctttta
    24661 tctgggccac aaaatcgacc attatcgtat aatactgcaa tatgtcgtcc gaaaaattgc
    24721 aatagaaact gttgcgcacg aaaatgataa gcatctgtat cgcaaacaaa accgtaaaaa
    24781 taaccagagc cagaaaagca aaagtctttt tcttcgatga gaagacgtcg tataattgcg
    24841 cttttatttt ctcggttagg ttgttgaaaa atccttcgat tttctgcttg gttttgacaa
    24901 tgttttcttt agctgtggtc tttccttttt tcatcatcgc gcctcgtaat tacctttacc
    24961 tgattgttga catattatat catatttttt ttctttaata tacacgtttt acggctctgt
    25021 ctatgaatca aagcttcttt gttgtataat tatctttggg tgatactatg gaaaaagcta
    25081 ttcttgtcgg ccttgatctc ggaacggatt ccgacttttt cgcttcgctg gccgaactca
    25141 aaaatcttgc ggaagcttgt gatgtcgaag ttgttggcca ggtaacacaa aaggccgatg
    25201 cccccaccgc taatttttat atcggtcaag gcaaagttgc tgaaataaaa caggagatca
    25261 atgtgcttga tgcgaatctc ttgatttttg acgatgaact tagtccgtcg cacattcgca
    25321 atttggaaca ggctttggaa ataaaaatca tcgaccgcac cgtgcttatt ctcgatattt
    25381 ttgccaaacg ggcaaaaacc aaagaagcga ttttgcaagt ggaactggca caagcggagt
    25441 actatcttcc gcgtgtcgcc ggtatgtata agtcactttc cagacaaaaa agcggaacag
    25501 gatccaaggg accgggagag caacaactag aactggacaa acggataatc cgtaaccggg
    25561 ttcacaaact aaaaaccgag ttaaatgaac ttgtggccgt tagaagaacc cagaggcaca
    25621 agcgctcaac cagcaacgtt ccgaccgttg ccattgttgg atacaccaac tccggcaaat
    25681 cgacactgat gaacgcactc atgtctcacg ctgaaaaaaa gagtgataaa tacgtcttcg
    25741 aaaaagacat gctcttcgca actttggaaa ctcaaacgag agaaataaag cgcgacaaca
    25801 atcacgattt tctggtgaca gatacggtcg gtttcatttc caaattgcca caccaattga
    25861 tcgaagcatt caagtcaacg cttgaggaaa ccactgaagc ttcattgatt ttgcatgtga
    25921 tcgacgcctc gaatccaaga cattatgaac aagcaaaaat aacggaaacc gtcctcgaag
    25981 aactgggagc gaaaaacatt cccataatcc atgtgataaa caaaatggat ctgtgtccgg
    26041 aaattccggc aaacgggttt tccaatccgg tttatgtgtc cgctcttaag gacgaaaatc
    26101 tcgaccggct tcttgatatg attgataaga cattgttcgg caaccgccat cttgtaaaac
    26161 tgttccttcc attcgacaaa ggtggcatat atagcgaact gcaagaaaat gcttttatca
    26221 agtcgacgga atacactccc gacggcatat ccgtcttagt ggaattatcg gaggcgttgt
    26281 acaagaaata tcaggaatac atcgcctgac ttctacaata acaacctaat ttgattcgtt
    26341 tttattggaa ctaaaggtcc gcttaataag gcggaccttt tttacaaact gtacaagtaa
    26401 tcgaaatact cttcgatcac taggctaaat tctttctcgg ttcttgtttg agaaagcctt
    26461 gcttttatct gggctgaatt tttaagtcct cgcaaatacc acggtccatg gcttcgcatt
    26521 tccaagattg ccgatcgttc cccttttaga tccctcaaca actcgaaatg acggtagatc
    26581 atttcttttc ttttttccaa cgtgacatgc ggttcgtatg tgcccgtctc aagatattca
    26641 accgtttgac tgacaagcca cgggtttccc aaaagacctc ttccgatcat tacggcgtcg
    26701 catccggttt gtttcaacat gtgttcggcg tcttccggtt tccgaatgtc accattgcca
    26761 acgacgggga tattcacttt ttccttaact tgacggatca catcccaatc tgccttacca
    26821 ccatacattt ctgttttcgt cctggcatgc acggcgatca tagatgcgcc agattcttcc
    26881 aatgcctttg caaatgacgg agcattcaaa tgttccttgt cccacccgct tcttattttt
    26941 acggagaccg gtttatttgt aaccgaaaca acggccctga caatggcgga tgctttttct
    27001 atgtccagca taagcttggc tccggctccg ttcttaacaa ctttagtcac cgggcacccc
    27061 atgttgatat caatgatatc ggcgtctgtt tcatttgtga cgatcccggc cgcaacagcc
    27121 atcgattccg gatcgcttcc aaatagttgc aaaacgatcg gatgttcgtc gggcatgact
    27181 gtaagcatcg aaaatgtctt ttcattttcg tgccccaagc ctttgtccga caccatctcc
    27241 gtatagatta aaccagctcc acattctttc agaatccttc gatatgccgg attggttata
    27301 ccggccatcg gagccactac cacttgattg agaatcgttt gtccggctac tttccattcc
    27361 atatccatca ctcgtttcct tatagccaaa ttatacctta ttttatgcct caagacaaag
    27421 aatatcgaca aatcccctta atacagaaat gttcggtaaa tggctatttt atgtgctttt
    27481 tcggtgtttt taaatcacgg ttgtttctat tggttttctc cattgttctt cctattgccc
    27541 agcttgcccc agttttttga ctcatatctg cttgttttag tattcaaaaa gaagtcttat
    27601 atcgcgattc gaaattgatt ttttttgttt tccaaaaaaa gaaagggcgg agcgtttttc
    27661 tcgcttcgcc cgcattgctt ttgtcagatc agtttgatat atttttttat ctcccactcg
    27721 gtaacagtag ttctaaattc gtcccattcg cggattttga gttctacaaa ttttttgaaa
    27781 atatgttcac caagcgcgtc ttttagcaat tcgtcctctt tcaagaggtc gattgcctcc
    27841 ttgagattgt tgggaaggct ggctaccccc agcgcttctc tttcctcatc ggttttttcg
    27901 aaaagattct cgttaatcgg agctatcaag gggagatttt tctcgatccc gtccaagccg
    27961 gaagccagaa tgacggccat cgccagatag ggattcgccg aggtatctac gctcctgatt
    28021 tcggttcttg tcgtccttcc tcttgttgcg ggtatcctta ccattacgga acggttgtgt
    28081 tccgaccagg aaacatagca cggagcctca tatccgggca ccaacctttt gtatgaattt
    28141 acggtcgggt tggtaatggc acagaagtgt ctggagtgtg tcaaaatacc tgtcagccat
    28201 tgccggcaaa cgagcgacaa ccccatcgga tccttcggat cataaaaggc gttttctcca
    28261 ttcatgttgg ctaaggaaca attcgtatgc atgccggatc catttatttt cgcaatcggt
    28321 ttaggcatga aggttgcatg taatccgtgt cttctggcaa tgtttttcac cactaacttg
    28381 aatgtttgaa tattgtcgca agcttctaca atattggaaa attggaaatt tatttcgttc
    28441 tgtccaggag cgacttcgtg atgactagct tccatggtaa accctattct ttccaactcc
    28501 aggacaatgt cccttcggca atcgccggct ccgtcgatcg gagacaaatc gaagtagccg
    28561 ccttcatcgg tgaccttcat tgatatctcg cctttttcat ccaatttcaa aagaaagaat
    28621 tccggttcga atccgatgtt caaagtggaa aagcctaatg cctccatttt cttggcgact
    28681 cttttcagat tgcctctcgg gtcggcttcg gacggtttac cttccggagt ataaacgtcg
    28741 cagatgagac gggccacctt cccgtaagcc gtttcctccc agcttagaat caaccaggta
    28801 tccggatcgg gacgcagata catgtcggct tccatgatac ggacaaaccc ttcgattgac
    28861 gatccgtcaa acatcacttc tccgttaagt gccgatttca accggtaaac aggaatctca
    28921 acgcttttga tcgtgccatt gatgtcggtg aacattaagc gcacgtagcg gacattttct
    28981 tcctcggcgt ttctgaggat ttgttcttta gtgtattttg ccatttgatc attctcatcc
    29041 gggttaaggg acaatgcaat gcactcccaa aaaccgaacg acctttcttt cgagacgcct
    29101 ttgtctcttc ataatatata ttgctattat accgaaacgt ttttttgatt caatatcatt
    29161 tcgttttttt ctgcaatatt tttaaagccg catcgtattt tccgattttt tcgtccattg
    29221 ctgtcttgag gattttttta tgggcttcat cttcgctcat ccctgtggaa atcaaatact
    29281 ccttgcattg ttgaaacaat tttccgtttg cgatttttga ttttgctttt ctgttctcgt
    29341 tctcaagcgc cttgttcttt tccagatatt tgtcgtacat gacaagaata accggtaatt
    29401 ccgaatccat tttcatctca tccgcgagca tgaacccggg ataactagaa agccgacgca
    29461 aaatagcgcc ggccggattt gacgacagaa aaacaacagg aatgtttttt cttgtgacta
    29521 tattttcggt aaatccgtaa agatctgtta tccggtatga ttcatgaatg acgacaatat
    29581 cgtacctgcc agcctcatcg ctttgaatcg gatgagaggc gatatccgga gaaaggccat
    29641 tggcacggag aatattttgg attctgattg ccaacaaacc gtcgttcacg cacgctagaa
    29701 tgcgccgaaa tttcatcgtc tgtatagcgg atggctgtca gtaagatctt tgactccggc
    29761 ttttactttc gccaatattt cttcgttttc cggattcatc aaagccatgt cgataagatc
    29821 ggcgactctg ataaaatccg cttccttgaa tcccctagtg gtcatggccg gacttcccag
    29881 tctgattccg ctggcgataa atggcttttc cttgtcgtaa ggaatcgtat tcttgttgca
    29941 agtgatattg actgagtcaa gcaatttttc cgctttcttc cctgtcaact ctgatttgga
    30001 tacgacatcc agcagcaaca gatgattgtc tgtccctcca gaaatcacat gatatcccaa
    30061 ctctatgaaa cgtttggcga atgcgtcggc atttgcgaca acctgttttt gatatactgt
    30121 gaattcgggt ttcagcgctt cttgcaaggc gaccgctttg gcagcgatga tatgcataag
    30181 cggtcctcct tgaattcctg gaaaaatgac ccgatcgatt tttttggcta tttcttcgtt
    30241 gttggtaaga atcattccgc cgcgtggacc gcgcagggtc ttgtgcgtcg tcgttgttac
    30301 gatgtcggca taatcacagg ggttgggatg cagcccggtt gccaccaacc cggctatgtg
    30361 cgccatgtcg accatgaaaa gcgctccggt ttcatcggcg attttcctta atctggcaaa
    30421 atcaatgata cgcggataag cgctggctcc cgccacaatc agccgcggtt tgatttccaa
    30481 tgcggttttt tccaattcgt cgtaatcgat tgtttctgtt tctttgctga caaaatacgg
    30541 atgaaattca taatcgatac cgctgaaact gagcggatgt ccgtgggtta gatgcccccc
    30601 ttggtcaagg gccataccca tgacgacgtc tcctttttcc aggacagcgc ggtaggcgcc
    30661 catattggca gatgatcctg agtggggctg gacattggca aatttgacat taaaaagttt
    30721 ttttgccctt tcgcgcgcta aatcttcgac tccgtccaca tactcgcatc ccccgtaata
    30781 tctttttccg ggatatcctt ccgcgtattt gttggtaaga acactgccgt cagcttccaa
    30841 tattgcttcg gaaacaaagt tttccgaggc aatcagttcg agatgcgact gttggcgtcc
    30901 caattcttct tcgatcaatg cggctatttc cggatcgaca gtttttagtt tatccataat
    30961 attcctcctt tatggaatgt gttgctttgc tattcaatta tatcatgcgt aaaccaaaac
    31021 gcccgcaaaa acttgggggc gttaaaagga aagaatcttt catgcggttt ttgatttgcc
    31081 aagagcatcc catacaatat cggacaaccg tccaaactct tctggcgaca acgcttccgc
    31141 ccggactccc ggagcaagat tggcttttgt gatcaatgaa tcggtttctg ttttgttggt
    31201 ctctggatag gctgttgcca aattgttgga taaagttttg cgtctgtagg caaacatctt
    31261 atgaataaaa tcataaaaga agctgtcgtc tttcacaata aaaggcggtt tgtttctgat
    31321 ctcgatatcg accacggcag aatccacgtt cggagcggga acaaataccg tgcgcggcac
    31381 cttgaaacga atctcggttt tggtccgata agccattatg accgacagag cgccatagtc
    31441 tttccctccc ggaccagcag acaggcgttt tgctacctcc aactgcatca tgaagatcag
    31501 ccttgtaact ttatccgatg tcttcagaaa tttcatcaga atcggcgtgg ttatataata
    31561 aggaagattg gcgattgcca taattttttt gctatcggga aaatgctcgt cgatctcttt
    31621 gtctatgtct atttccaaaa tatctttatt cattatcgta atgttatcga ggcttttcaa
    31681 tctttctttt aatatcggaa ttaacgtgct gtcgatttca tatgccacca ctcttccggc
    31741 tttctttgcc agaagttcag ttaaatttcc cgttcccgga ccgatttcga taacatcggt
    31801 ttctttgtcg atttttgccg catcgattat tttattaaga atgttgcgat cggaaagaaa
    31861 gttctgaccg aacctttttt tcggtttgac ccccatggcg gtggttttat ctttcatttc
    31921 agacactctc gtcttgatga tggaaataac gtttttttgt ggctggcggc acgattatcg
    31981 agataccatg ccgacaaaca tggatgtttt gatttccgac accgcccgat tctccatcga
    32041 tattccaacg tgaacgggtg ttcgtaacta tcttcaaaga agacgttttg aatctagtga
    32101 caagcggagt cgaccaaaac ggtaaaagga aagacaggat atatagaatc cagttgagag
    32161 gcaacaaact cttatacatg acaacattta caagcccgtc gtcgagactc ggtttgtaaa
    32221 tcatattgat gccggccacg cgtttggaat tgataatcaa aacaagagaa taaacgcccg
    32281 ataccgttcc gtggtcgtgt gttacccgca tccgcatttt cggaatggtg aaaatctctt
    32341 ccgccgcttt gatgacgtag gcaaaatatc cgatcttttt tttcagtttg gaatcggtga
    32401 catagcttat atcgatgaac gccccgtttc cggatacgta acaaaaataa cggtcgttgg
    32461 cttttaccac atccattttc acaggagtgt tcgtcaatat gttgttcacg gccttgtcga
    32521 tatttttcgt aaggccgaga gtcatcccga tatcgcagca ggttccagcc ggaatatatc
    32581 cgatagtcgg tcggttttca aattccatta gtccgttgac gcactcgttg aaagtgccgt
    32641 ctccgccgac gattataact tcttcgtaac ctttttgaca cgcctcccga gccaggattg
    32701 ttgcttgtcc gggctttccg gtcgcatatg cttcgatatc atatccggcc gcccggaaac
    32761 gcgcgagcgt ttcttccaga cgtccgacga atttttgctt tccacttgca gggttataaa
    32821 tcatcatcgt tttcttcaca aaaatcaccg ataaaattat atcataatta aacacataac
    32881 accataatct acaatacaga attgttttgt ctgtcatgtc cggggttttt tgttataata
    32941 ttatcggtga taaatactat gcgcaagatt gaagccgtac tttttgacct tgatgggact
    33001 ttggtcgatt ccaacaacat catcattgat tctttccgtc atgtcatgac tgcttttttc
    33061 ccggaacgat tatggtctcg aaaaaacatt atggaaatga tcggtcctcc gttatacgac
    33121 actttcaaaa aacatgcttc ctcggaggaa atgatttccg atatgatttt tgcatatcga
    33181 aaatactatg tcgaaaacga atttgacagc atttcgcttt atcccggggt aaaagaaact
    33241 ctaactgaac ttaagaataa gaattgccgg ctcggcgtgg taacaaccaa attccgtgtt
    33301 tccgcccttc cgtcccttga acatttcgga ttagacaaac tgtttgaaga aatcgtatcg
    33361 cttgatgaaa tcactcacgc caaaccccac cccgagcccg ttctctatgc tctgaaacaa
    33421 cttaaatgcc acttcggcat aatggtgggc gatcatccgt ctgacatcgt ttctggcaaa
    33481 gctgccggaa tcgctacgtg tgccgttgac tggtcgctca aaagaaagga acttgagctc
    33541 tcttcaccgg attactggat caaagatttc cgacaattga ttaaaatcgt tgacgatata
    33601 aataaggagg aatgacaatg gattgtattt tttgccggat cgcaaacggc gaaattcagt
    33661 cttacaaggt atacgaggat gattgtttca tcgctattct cgacatctct caagcaacca
    33721 aagggcatac gcttttgatt cccaaacaac actttgcaaa cgtgtatgaa atcgacgagg
    33781 aaattattgc cagaattttt aaaaagattc ccgaaattgc caaggctatc aaaaaagctt
    33841 tcaacccgat cggactcaac atcctcatca acaccgacaa acctttgcaa tcagtcttcc
    33901 attttcatgt ccatcttatt ccgcgctatg aaaacgacgg agttcgcatg gaatttgaaa
    33961 acaactacgg aaaattgccg gcggaagaat ttttgaaaat cgccgacgaa atcaaaaaga
    34021 atctgtgata aaaaagaaag ggaaccgaag ttcccttttt tatttgctta tgatgtccgt
    34081 gcccatgtat tttcgcaata cttccggcac ttcgatcgta ccatcggcct tttgatagtt
    34141 ttccatgacg gcgaccaatg tgcgtccgac cgcaagtccg gatccattca aagtgtgaac
    34201 gtattccagt ttgccgtcgg tgtccggccg gtatttgatg ttggcgcgtc ttgcctgata
    34261 atcctcggcg tttgataccg aactgatttc ccgataccgg ttttccgaag gcagccaaac
    34321 ttcgatatca taagtcttgc acatcgagaa ccccagatcg ccggcacaaa gaacaaccac
    34381 atgataaggt atcttcaata gcttcaggac atcctcggcg tcttctagca tccgcatctg
    34441 ttccttatac gattcttcag gcttagtgaa tttgatcagt tcaaccttat tgaactgatg
    34501 ctgtctgatg atgcctttcg tatcttttcc tgccgatccg gcttccttac ggaaagcggt
    34561 tgtgtaagaa acatatttta tcggcaacaa atctttgtcg atcacttcgt cgcggtggag
    34621 attgatagtc ggaacttcgg cggtcggatt cagataaaga tttctgtcat cattgacttt
    34681 gaaagcctcg tccatgaatt ttggaaattg tccggttccg tacattgatg tctcgtttac
    34741 cagatagggc ggcattattt ctgtgtagcc atgtttatta gcgtgaagat ccatcatgaa
    34801 ctggataagc gatctttcca aacgggctcc aagtcccttg tatacgacga agcgagctcc
    34861 ggatatttta ccggcccttt gaaaatcaag gatgtcaaga gcttctccga gatcatagtg
    34921 gcttttcggt acgaaatcga attgggttgg ctcaagattg cgtttgattt ccacgttttc
    34981 actgtcgtct tttccgtttg gaatatcatc tgccggcata ttcggtgttt tcagaagtgt
    35041 ttcgcggata tcgtcatcat attgtcttac cgcatcttcc aacccggcga tcgaatggga
    35101 aaaatcatcc atgtttttca gaatcgattc gaccgatttc ccttcgcgct tgaactggcc
    35161 gatctgtttt gatatttcat tctttttagc tttcagcgct tcgacttcca aaatggcttc
    35221 ccgtctttta cggtcggttt ccaacatgtc gtaaagatag gaaaagtctc cgccgcggcg
    35281 gttcaatttc ccgatgatca attccgggtt tgcccggatt tgtttgatgt ccagcatgga
    35341 tatctttcct ccttgatatc aataactgtc ttttagaatg tcgtcaacgt ccgattcttc
    35401 ctcgtcgtct tcctcattct cttcttcgtc ttcttcgacg aagtgattca gttccttgta
    35461 ttcttccata tggaattctc tgattggttt ttgctgttcc tgatttgccg agatattatc
    35521 gtttgtttct tctgtaattt cggaattgtc atctatggat tccatatttt cgtcatcgtc
    35581 ctcttctttg gggacgatgg cgattgtcgc cacttcgtga ccatcgagga cgttaatgat
    35641 ctttacgccc tgggtagatc tttttgtctg ggctatctga tcaatcggaa ctctgataat
    35701 catgcctttg tcggtcacaa caagcagatc ctcgtcacca accacgctga tcagtttctt
    35761 gagacatccg ttcttagtgg taacattgat cgttttgaca cctttgccgc cgcgtccttg
    35821 aagtctatat tcgtcgacat cggttctttt tccatatcct ttggcggtaa tggcgagaat
    35881 ttcgcttctg tcgacggtga cggtggtcac tccgatgact tcttccttgc cagacaaagc
    35941 aattcctttt acgccggccg catcacggcc catgctacgc acgtcttgtt cgttgaatct
    36001 gatagctttg ccattggatg ctccgataat gacgtcccga tttccgtcag tcaattttac
    36061 gctgtgcagc tgatcgccgt cccgaagaga caaggccctg attccgttgg tcctgatatt
    36121 ttggaatgcc gacacttttg tgcgttttac aacgcctttt gttgtagtga aaaacaggta
    36181 tccttcattg aagtccttga cgctcatgac tgcagccaac ccttctccgt ccgtaaggtt
    36241 gagaagattg acaatcggga gtcccttcga attgcgtccg taattgggta tcctgtaccc
    36301 ttttatttta tacacgcgcc cgagatttgt aaagaacagg aggtaatcat gtgtcgaagt
    36361 cgatatgatc gaatcgataa catcatcctc gtttatcttc atcccggttt tgcctcggcc
    36421 tccgcggttt tgcgatttgt agacggcgac attcatccgt ttgacataac cgttggtcgt
    36481 aacggcaacg attacgtctt cgataggaat caaatcctca tcttctatgt cataatcgcc
    36541 cgaaagatcg atttccgttc ttctggcatc tccatactta tttttgacat cttcagcttc
    36601 ggaaaccaat atttcgtgtt gcagttcctt gtcgacgaga atttggttgt aataattgat
    36661 cgctttttcg atttcggcca attcggcatg aatcttgtct ctttccatgc ctgacagttt
    36721 tctcagttgc atcgccaaaa tggcttttgt ctgaacatcg ctgaaaccat agcgctcaat
    36781 cagtttttgt tcggtatcat catatgattc acggattatt ttgatgactt catcgatatt
    36841 atcgagagcg atgatgaacc ccttgagcag atgatctctt tgaatggctt tgtccaaatc
    36901 gaattttgtt cttctgatca aaactgtagt ctggtgttcg aaataatatt ttattatttc
    36961 cttgagcgaa agaatcatcg gtttgtcatc cacaagcgca agcatgttca cgccgtatga
    37021 tgcctgtaat tgcgagttct tatagaggtt gttgagaatg acttccggat tggcgtcttt
    37081 tctcaattcc aacacgatac gcataccgtt gcgattggat tcgtcacgga gatcagtgat
    37141 accttcgatt ctcttgtcct tggcgagttc cgctattttg tctatcagat ttgatttatt
    37201 tacctgatac ggtatttctt tgatgatgat ctgttttttg ccggaagcca tgtctttgat
    37261 ttcggctttg gctttgaccc tgattgttcc gtgtccggtc aaataagctt ctctgattcc
    37321 tgacattccc aaaattattc cgccggtcgg aaaatccggc cctttgatat actccatcag
    37381 ttcttcgtct tcgatatcct gattgcgtat ataagcgata tatccttcaa ttacctccgt
    37441 aagattatgc ggcgggataa cggtggacat tccgacggcg ataccattcg ctccgtttac
    37501 caacaggttg ggaaatctgg ccggtaatac tttaggctcc tgttcgctac catcgtaatt
    37561 atcgacaaaa tcaaccgtat ccttgcggag atccttaagc atttcgacgg tgattttttc
    37621 cattttcgct tcggtgtaac gcatggcggc agcgccgtct ccgtcaaccg atccgaagtt
    37681 cccgtgtccg ttaaccaatg gataacgata actgaaatcc tgagccatcc taaccattga
    37741 atcatagatc gccgaatctc cgtgagggtg atatttaccc atgacgtctc cgactattct
    37801 ggcggatttc ttatattgtt tatctggata cacgccaagt tcgtccattc cgtaaagaat
    37861 tcgtctgtga actggtttca atccgtctct gacatcaggt agcgcgcgag caacgatgac
    37921 gctcatagca taactcaaga aagaagtttt catctctccc gaaatattta cttcctgaat
    37981 tttatctttt atatttgtgt tttcgtattc ttgattttcc atgatgtccc cctaaatatc
    38041 caggttacgg acatattccg cattttcctg gataaactcc ctgcgactgg ggacatcgtc
    38101 gcccatcagc atggaaaaga tcctgtcggc ctcgatggcg tcttccagcg taacttgcaa
    38161 gagtgttctg gcttcgggat ccatagtggt ttcccaaagc tgctccggat tcatttctcc
    38221 gagacctttg tacctttgca agaccggttt tccggtcaat ccggcaagaa tcctttcgag
    38281 ttctttttct tcatatgcgt attgtataga cttgccaagt tgaattttga atagcggcgg
    38341 ctgcgcaata taaatataac ctttttcgat cagcggcctc atataacgaa agaagaaagt
    38401 caaaagcaac gtgcggatgt gagctccgtc aacatcggca tcggtcatga tgataatctt
    38461 gtgatagcgc gcctgatcaa tttggaattc ttcaccgatt cccgttccaa acgcctggat
    38521 catcgataca atctctttgt tttgaagcgc acgatccagt ctcgcctttt ctacattcag
    38581 cacttttcca cgcaaaggaa gaatggcttg gaactccgcg tcacgacctt gttttgcaga
    38641 tccgccggcg gaatctcctt caaccagata aatttccgat tttgccgcat ctttggtccg
    38701 gcagtcagct aatttggaag caaatcccag ggaatccagc ggggatttcc gcctggttgc
    38761 ttcccttgct ttcttggcgg cgattctcgc cctctgggcc atcaaggctt tttcaagaat
    38821 atttttggcg gcagcggggt tttccagaag gtatctgtcc aacccttccg acatgatatt
    38881 cgcgactact cttctggcgt cgctcgatcc aagttttgat tttgtttgtc cttcgaattg
    38941 aggatccgga tgcttgacgg aaacaatggc cacaagccct tccctagtat cttctccggc
    39001 caacgattcg tctttacgga aaacgttagc gcttttgccg taagcattta gaatacgcgt
    39061 cagggtcatt ttgaatcctt cttcgtgcgt tccgccttcg gcattgttaa tgttgtttgt
    39121 gaaaggatat atattcgcgg cataaccatc gttatattgc atagcgattt ccaccgttat
    39181 gccctcttgt tcgccttcga aataagcaac ttgagggtta agaaccgctt tacccaaatt
    39241 caaaaattcg acatattccc taacgccgcc ctcgtatttg tattcttttc ttactgtgct
    39301 ttggggattg cgtaaatcgt ttatgtcaaa ctggattccc ttatttaaaa aagccagttg
    39361 ctggattcgc gatctcaaag tttcaaaatc atattcctga cattccacaa acactttagg
    39421 gtcggccaaa aacgtgacaa tcgttcctga tttatctgta tcaccaatgc attccaattc
    39481 tttcttcaaa aatccgtggt caaaagagat ggtgtatatt ttgccgtctt tgtgtatttc
    39541 gatatccata tattcactca aagcatttac taccgatgct ccaaccccgt gcagtccgcc
    39601 cgacaccttg tacgagttat ggtcgaattt tcctccggca tgtaaaaccg tataaacagt
    39661 ttctacgctt ggccgtttgg ttttcggatg aatatcaacc ggaatgccgc gtccgttgtc
    39721 ctcgacccgg acaatgttac ccggaagaat atcgacgatg atgcgatcgg cataaccggc
    39781 caaagcttca tcgacggaat tgtcgacgat ttcccacacc aggtggtgca gtccgcgcgg
    39841 tccggtggta cctatataca tccccggtct tttctttaca gcctccagac cctctagaat
    39901 ctggatattg ctggcatcgt attttgcgtt gttgtgatca gccatttcag tactcctcct
    39961 tgatatatcc tttgttgaca tgaaaaacat gtgcctgttt caaaataaaa tccttgactc
    40021 cgccaagccc cgtggtggtc actattgttt gtattcccga agtcgccaca tattccaaga
    40081 gccggttttg tctggtttca tccagttcgg aaaacacatc atctaacaag aacaccgggg
    40141 tttcgtgcct tgtctctttt acaagctctg ccaaacccat attcagggac aacaccatca
    40201 ttcgctgttc tccctgcgaa gcccggctcc cggcggcaaa ttcatcaagc atgaaaacgt
    40261 agtcgtcgcg gtgcggacca tatcccgtcg cgccctgcat gatgtcctga cggtattttt
    40321 ccttgagaaa tccataggct tccttttgtg gcaaagtctg ttgatagaca agagaaaatt
    40381 tttcgtcttt gtcggaaagt cttttgtaca ttccatcgat caagccgttg atttcttctt
    40441 gaaaccgcac tcttctttga ctaatctttt ccgccgaaga cgccaactgt tccgttatga
    40501 catccaataa tgtgtaatcg ggtttttcaa catattccaa tttcttcagc aattcatttc
    40561 tttgtttgag aatatgttta tatatcgtca gttcattcag ataatctttg tcgatttgcc
    40621 cgagaaacac atcgacataa taccttctgt cccttggcgc tcctttaatc aaaacgatgt
    40681 cttcaggcaa aaaggaaacg atattcagat tgccgatgta atcgctgagc cgttttatct
    40741 cctggctgtc gcgcatggct ttttttccta tttctgtgat gatcattgtc aattcgaatt
    40801 tgcgtccgtc tttttcgatc gtcgcctgta ttctggcaaa atccgcactg aatcgaatca
    40861 gatcgctgtc ttcggcctta tatgattttg ccaaagcaag cacatacaaa gcttcgagaa
    40921 agttggtttt accttccccg tttgccccga taataaaatg aatacccgaa ccaagttcca
    40981 gggtctgttt gtgatagttc ctaaaattgg ttagggtaac tttgtctatt ttcattcttt
    41041 gttgacaacc ttcaggatga ttttattttc aatttcaacc agatctccgg gataaatttt
    41101 tttcccgcga cggttttcca atgctccgtt gtgagttatt ttgttttctt ctagaaagat
    41161 ttttgcgtct cctccgcttt ggaccaatcc ggaatacttg attagttgtc ccaaggtgat
    41221 gaagggggtt ttgatattga tcgttttcat cgatccacct ctttgcggag ccaaatcaaa
    41281 actactttta tatataatat tatataatat tattatataa tttacaactt aattttttta
    41341 tcgtcaaacg gtcgacgatg cctttttact ttatagtcaa aagaaaaagc gcgtgttttc
    41401 acgcgctctt atttgttttc cgaaacgatc ttttccagaa aatccaccat ggtgtataac
    41461 cagtgttttt caagtcccac cgtatctaca attatgctta tcttgttgtg ccggtatgcg
    41521 aaccgaatgt agttggatat ttccaaaccg gtcgtaaaca atgcttcgcc tgacattttc
    41581 ttggtgccgt cttcagaaat gaccagcgtc acgtttgttt tgctttctat tagtttttct
    41641 ccgtcgatca tcttcgaaag tttttcaaac aacttctcgt aaaggtatat ttccagttcg
    41701 ggaatatatt cgccaaatcg gtccttgaat tcggcaagca aatcagcaat atcctttttg
    41761 ttttttatcg aattgatttt tttatacatt tcgattttaa cgaaatcatc attgatatat
    41821 tcttcgtcaa tatatcttga caccgtcgct ttgattcctg gtttggtttc cttgatttcc
    41881 ggttttcctt gcttggcatt tatttcttct tcaaggatcc gcatgtacat atcaacccca
    41941 accgaatcta taaatccgga ttgctcgctt ccaagaacgt ctcccgctcc gcggattgac
    42001 agatcccgta ctgcgatttt gaagccgctt ccgagttctg tgaattcttt gatcaccttc
    42061 aaccgtttct cggcgtcctc ggtcaaaact cgatcctttt taaacatcag atatgcgtat
    42121 gctattctat cgcttcttcc cactcttccc cggatctgat acaattgcgc tagtccaagt
    42181 ttatcggcat catgtatgat cagagtgttg acgttgggaa tatctattcc ggtttcaata
    42241 attgtggttg ctaccaaaac atcggttttg cggtcgataa aatcgtcgat tacggcttcc
    42301 aattccagtt tgtgcatctg cccatgggcg atgcaaacac gggcttctgg aacgagtttt
    42361 tgaattttta tcgcaatgtt ttcaatgtca tccaccctat tgtacaaata atatacttgt
    42421 ccttcgcgag ccaattctct ttctattgca tctttaatca cggcatcgtt tctttctatg
    42481 acatatgttt gtatcggata tctattctct ggcgcgcttt cgagaaggct catgcttttt
    42541 actcccatga tagccatttg caaagttctc ggtatcggag ttgcggataa ggatagcaca
    42601 tcaacattaa ctttgaactc ctttattttt tctttatgct gaacgccaaa tctttgttct
    42661 tcatcgacaa tcagcaaacc caaatctttg aatgctacgt cttttcccaa aaccctatgg
    42721 gttcctatca caatatcgat gctcccgttt tccaatccag ttaagatttg cttttgcgcc
    42781 ttaccggtga caaatcgatt aagcaacact atattgactc cgtatttttc caatcgcgaa
    42841 gtaaaggttt gaaaatgttg tttggataaa acggtcgtcg gagcaagata tgcaacttgt
    42901 ttattattca aaactgctct gaacgccgcc cttaaggcta tttcggtttt gccaaatcca
    42961 acatcgccac ataacagtct atccatcggt cgtggcgatt ccatatcgga aaacacctct
    43021 tttattgctt tgttttgatc gcgggtctct tcataaggaa agtcgatttc caattccgat
    43081 tccaattccg cgtttggtcg gaaagaatac cctttagctt tttctctctg tgaataaagt
    43141 ctgatcagtc tttcggcaat atctccggct ttgcttctga ctctttgttt agccttgttc
    43201 caatccgatc cgcccagttt agatagtctg ggaacaaacc cttcgcttcc ggcatatttc
    43261 tgcaagtcag aaaatgactc caagggaata tacagcttgt cgccatctcg atattcgata
    43321 tttatatagt cgtttctagt attgccaagt tccattgttt ttatatccag aaaacgaccg
    43381 atcccgtaat cataatgaac gacataatcg cctggtttca aatcattgac agatgctagc
    43441 cgcttgcttg aatcgaaagc ggaaaggtat tttcccttgc gcttcgaagt ctgctttttg
    43501 ttaatggatt tttcagtaat caccgtcaac ccgaacgcct ccgacgcaaa gtcgaagcat
    43561 cggtcggaca cgataatgtt tatggaattt tctttaagcc tttcagtgtc tccagttagg
    43621 acataatcca gtttattgtc ggaaagcaac tcgcacatct cggttctgga cttatatgat
    43681 tccaaagcta tcacaagtgt ccgttgtccc gcgagagatc tcaactcatt caagaccaga
    43741 tttatatttc cttcgaattt tagcggttca ttggctgcga ttgaaatttt ttttatgttt
    43801 tgcagccgcg tttcttcccg aagataatcc acataaattg gtttttcaat ctttatattt
    43861 tccaaaggca cgaaaaaatc gaatcccatt tttgcatagt ctccggcgtt ctcaatccag
    43921 tccagaagat cttgtttcaa gccgtcatat tgcccgttga atctatgaaa atcaaaaaag
    43981 aaaactgtgc ttttagggat aaaatccaga attgtatttt gttggccaaa tagaaacttc
    44041 tggtatctgg aaagcctctc caaatcttca cgtctttcca aactttctaa atccgcatca
    44101 atcttttcga ttgcttccga agatagtttt ttgtccctga ttattgtttt aacttcgttt
    44161 tttatggttt ttaacccttt gtcatcgaaa aacaattctg tcattggcat aacatcgatc
    44221 ttttcgcacg attcctgtga tctttgcgat tcaatatcga accgtttcat cgtatcgatt
    44281 tcgtctccga agaaatctaa tctgaccggg gttttttctc ctaacggaaa gacgtcgact
    44341 attcctcccc tgacactaaa gtctcccatt ttttcaaccg tgtattcgtg ggaataacca
    44401 gacatgatca attttgaact aagtttgtct attgatattt cctgttttat gcaaaggcta
    44461 aacatggcgt ttttccacgc ttctggctcc agttgaggtt tgatcagtcc tgggagatgg
    44521 gtcacgacta ttgcgttgtt gttttcaatc agacgcttta tcgtgttgat tctttcaatg
    44581 cgaaattccc aagacgagac cagcatctcg ctggtaatga attcgtcttg cgggaagaat
    44641 aaaacttctt ccggatcaag ggctgcggaa agtttatcat aaacaatctg ggcgtgataa
    44701 aggttggggg tcacaacaac gatcggcccg ccggcctgtt tgtgtgccga caaaaccaaa
    44761 aacattccgc cctcttcggt tgtgttgctg atttgtatcg ctttgccttg ttgtttgatt
    44821 gcttctgaaa actctttgaa ttcttttttc gcttccagaa attctaaaat tttttccatc
    44881 attttacctc acgtcgtttt ggcgagtctc acgactcgcc gggggtatag cagatattca
    44941 tattttgatt tctaacgttt tttcatttga tatctatacg aaattgccat aactgttttc
    45001 tttggaaaaa ctcttagagc aactctggca atcttcataa gcgttcctgg aatgatgatt
    45061 gtttttttgt tatcaagatt tcttatcgct atctctgcac atttttttgg tgttattcct
    45121 ttaaaggcgg ttttgactct cgctacatcg gcaaattcgg tttccaccgg acccgggcaa
    45181 agtattttaa ttctcacggg ttttttctgt tggaccagtt cactattggc ggcgagggaa
    45241 taattgagta cgaacgcctt tgaagccgcg tatgccgcca tcaacggagt tggtaaaaat
    45301 gccgccatac ttgccacatt caagatgagg ccccgttcca ttcccgaagc gaatagtttg
    45361 gttaaccgag aaagtgaatc aatattcagt cttatcattt cgctttcttt ttccggagga
    45421 atttcttcaa atgagccata ttgtccgaag ccggcgttgt tgataacgat caagggctta
    45481 taagttattg tctcgtcata aagcttttga gtgttttcag gcaaggagat atccatcgct
    45541 ttgatcacaa catcagttct ataattggat gtcaattcta ttttcaactc ttctagtctt
    45601 tctgctctcc tggcaaccaa aatcacgttg tatccttttt ttgccaaccc tctcgcaagt
    45661 tctcttccga taccgctaga cgctccggta atcaaaacaa acggtttccc ttccatggca
    45721 aaacaactcc ttcgtcttct attgacatta taccatcata gtgacgcgaa aaaaagcgtc
    45781 gtgtattttc aaacaaaaaa accgtctgtt tcgacggttt tcaattatat ctgttcatcg
    45841 tttcttcgaa tggttttccg ttctggaagt catcgattat tcccatgcac tctatgatgg
    45901 tatcatctaa cattttactt tccgctttgg aaaatccgct tagcacatag tctactgtat
    45961 cattttcttc gctgtgtcca attccgattc taacccgttt gaatgcttcg gttcccattt
    46021 gcgagatgat tgatttgagt ccgttgtgcc ctccggatcc gcctttttct cttaggcgaa
    46081 tttttgcaag cggaagatcc atgtcatcgg atatcaccat tatttcctca accggaatct
    46141 tgtaaaatcc cgcaaccgcc ttgacggagt ttcccgacag attcatgaaa gtttttggct
    46201 tcagaaatat ggtttctcct atagtcaaca cttctcccat gaattttgtt tctttggaga
    46261 acttggcttt ctttgttttt gcataggcat caaggcacat gaatccgaca ttatgccttg
    46321 ttttggcata ctttcttccc gggttgccca atccgacaat catgcgcttc atgctattta
    46381 ttcttgggtt tgattacgac gtaacggttt ggctcttcgc cttcggattc ggtgatgaca
    46441 tcgcgccagt ccgctaattt cgtatggatg attctacgct cgtaagcatt cattttcccg
    46501 agtttggcgg gcactttcgt cctagcaact tcttttgcgg ttttagtggc caaaatctcc
    46561 aattgctttt tcttctgatt tttatatccg ccaacatcga cggaaacaac ataatactcg
    46621 tcagtgaaaa gattgataaa gttttttatc agagtctgaa ccgcatccag ggttttcccg
    46681 tttttgccga taagaagagg attttctttg gcgtcaatcg aaaaggtcaa ttgtctttcc
    46741 tcttcggctt ttgcctcgac gatggcttcg atttccatgg cggtcaatat ttctttgaga
    46801 tattcgattc cgtcgcgtag gggatcaaac ttgattttgg cttctacaat gtaagtcttg
    46861 tttaatccaa gaatactctt tttttcctca accacctgca agtcgacgta tttacgattc
    46921 acatgcaact cttcggctgc gtatttgtat gtggattcat taagggtttt ggcctcaaat
    46981 tggattgatt tcattcgatg tccatctcct ttagaatttg cttctcattt tttcttgttt
    47041 gctttcaatg tttcggcggc tcagataact ttgcaacagt tgatatgagt taccaagaat
    47101 ccagtaaaac gcgataccgg cattgcttat ggcgatatac cccatcatca ataccatgaa
    47161 atagttcata tatttcatca ttttctggct ttgctgggct tggctgtttt ggtacttgtt
    47221 taccttctgg ttttttctcg tttgtttttg aatcagccat tggctcaaca tcatagttgc
    47281 cacaacaacc agcgccaatg ggagattttc ccagaaaccg gtattgccaa gatccgtcca
    47341 aaggaaattg taattcatta tcgcgtcggt atttccgaaa gccgataccg gcgtaatggg
    47401 gaaccgtcta accacggtgt acatggcgat gaatatcggc atttgcaaca aaggcatcaa
    47461 acatccgaag aagttgatct tgtattttcg ataaacttcc attgtttcca tctgcatgcg
    47521 ttgttgcgag gcttgatcgg atcttccttt gtatttttcc tggatccttg ttatttccgg
    47581 ctgggcaatc tgcattttca gtgtcatgtc gttgctcttg gaataaatcg gccaggccaa
    47641 ggttctgatc acaagggtca ttatcagtag tccgagccag tagtaataag tttcgcccaa
    47701 taaaccgaaa aggttgctgg catagaaggt gaatttggcc acaaccatga caacccattc
    47761 cgtccatgaa tctttttctc cgatcggaag aagggtgccg gaagcgctta gtccttcctg
    47821 ggttttactg acagctttat aagttgtaaa cgtgactgtg acggtggcgg tatcagttga
    47881 ccaaacaacg actccgtctc cgttagcata accgctctcg tcacttggag ttcccagcag
    47941 attgatcgct tcatagtagg acgtggcatt tgtttcatct tcagaaaccg tgacgatgct
    48001 ggtataattc tcatagctga cttcggcttt gctgccgcac gcagagagag caaataccaa
    48061 cacaaatagc aaaattgcag caatgatttt ggtttttttc atgtcattcc tatcctttat
    48121 gttatttttg ccttgacaaa caaaggcgtt aaattgtctt tgatttcctt aaaatctagt
    48181 tttcgggcag ttggtttcgc gacaatcaaa aagtcggttt tagccgttat gtttgcttgt
    48241 ctggttattt ctctgattct tcttttcatt ttgttccgct caacggcgtt tccatatttt
    48301 tttgatacgc tgacggcaaa acgaaaatga tcgatcttgt tgactgcggc gtacactacg
    48361 aaaaaaccgt tggccgcgct gtttctttgt ttgaccagcg cgtctatttc ttgattcttt
    48421 ttgatgcggt atgccttttc caacccggtt catccccttt aaaaacaaaa atcaccaaaa
    48481 tatattatat tcaggtgatt acaggtttta atataaattg gttatgaaat tatttggatg
    48541 tggactgatc ggaaaccgtc aaggaataac gtcctttggc tcttcttctt gccagtactt
    48601 ttctgccgtt cgcagattcc attctggctc tgaaaccatg ggttctgatt cttttgatct
    48661 tgctcggttg ataagttctt ttagtcattg gtgacacctc caaagcttgt tcctaagcca
    48721 tgctacataa ttatacggga ttttcaaaaa gaagtcaata tctttcgctt tggagaaaaa
    48781 agcctttttt attttttgaa agcacttttc tgtttcttgt gttttttcct tttttttact
    48841 tgttaaattt cttttcaaca aaggtttttc aacttgcttt ttaaacattt tacggtcctg
    48901 ttttttccag tggaattgtg gaaaacccac tttcttcgcc ttctttcggc tgtggttttc
    48961 cacattttgc ttgttctttg atttttgctt gttgaagttt tccacaaatt ttgtggagaa
    49021 atagttcaca tttttgttgg gcgttatgat atatttagga ttatagaggt agccgcctgt
    49081 ggaagaattt caaaaaatct gggataaaat caaatatcag cttcaactca ctttggatga
    49141 ggacgtttac aacgagcatt tcgctccgat ttccaacgtt tttaaagttg taaataatta
    49201 tgtttatctt atttctccta actctttcat taaaagcaaa attgaatcat tctacttgaa
    49261 tcgactcaat gccatgctga acgagcatgt ctcggaaaag cacatgttca aaattatcac
    49321 caaggaacaa gccgccgaag aaattgccaa aacctccacc tatgaaatca atattcccga
    49381 acaaggattg gaaaacaaat ataaaaccgg actcaacaac tcttatactt tcgataattt
    49441 tgttgtaggt tcaagcaacc gccttgccta tacatcggca atgaaagttg ctgaccagcc
    49501 cggcgaagtt gccaatcctc tttatatatt cggtgacgtc ggcttgggaa aaacccatct
    49561 gatgcagtgc gtcgggaatt atatattgga aaacaacgtg aataaaaagg tcctctacgt
    49621 aaaaactgat caatttatcg aggattatgc caaagccgct caaaaaaaga gtttcgacga
    49681 tttttccaaa aaatatgagg atgtggatat tcttctggtt gacgacatcc agtttttatc
    49741 cggaaaaacc caatcccaat tggaattttt taagatattt gaaaaactca aagaaaacaa
    49801 caaacagatc gtgataactt cggatcgcca ggcaaaagaa ttatatgata tcatgtcgcg
    49861 tcttacttcc aggttcgaat ggggactgtc agtagatatt aatcgcccga atctagaaca
    49921 tcgcattttg attctcaaga aaaaacttca agcggagact gccgatcccg aactgattcc
    49981 tgaagaaatt ttggattata tcgccagtat ctttgacaac aacgtccgtg aacttgaggg
    50041 cgctctgaaa agggttttat tctattgcac cgcgtttgat tacaaattca cgatcaataa
    50101 cgctcaagat gcgcttaaaa atttggtcaa ttccaaaaat atttcaaata attctatcga
    50161 aagaagcaat ttcaataacg ttctcgacat cgtcagttcc tattataata tctcgaccgt
    50221 cgacctttta tctaaaaaga ggaaaaaaca gtatgtcttc ccgcgtcaaa ttgccatgta
    50281 tatccttaaa gatctgtatg atcttacata caaaaaaatc ggtcaaatct tcaataaccg
    50341 cgatcattcc accgtcattt acagcatcga acagatttcc cacgatattc aaactgacaa
    50401 cacaaagaaa actgacatcg acaaactgtt gataaaatgt ggaaaaaaag ggtaaatcca
    50461 cacagtccaa agattgatat atctatgtta ttttggccaa atatggtata atgataacag
    50521 atataatttc acgttttctt cttttccaca cctataacaa taataataaa aagaaaaaga
    50581 taataaatgt catataggag gctgtccatg aattttatca tttcgacaag cgtgctgctc
    50641 aataatctgc ttatcgctca aaaagcctta tccactaaaa ccccggctcc ttatttgcag
    50701 ggaattaaga tggaagttta ccaaaaggaa gttgttctag tcaccagtaa ctcggacata
    50761 tctatcaaac tgtctatcaa agacgaatct ctgaaagtcg aatcaacggg cgaagctttg
    50821 gttccgggaa gatttttcgt tgacataatc cgtaaggttg aaggagaaac agcggaactt
    50881 tccgtcatcg acaacaacat acttcgtatt gttgccggcg aatccgacgt cgttttaaac
    50941 cttctcaaca tcgaagatta ccccgaattg gaatttccgc tcaatgacga accgattaaa
    51001 ttggattcga aaatcatgaa ggcgatcatc agacaaacca cttttgctac atccacgatt
    51061 gaaaacagac cgattcttac cggtgtgaat attcgcgtcg agggagaaaa gttaatcgcc
    51121 atcgcgaccg attctttccg gctgagtcaa aaagtcgtcg acattccgaa gaacatggac
    51181 aacatgaacg tggtgattcc cggaagaagt ttggacgaac tggcaaagat attggaaatc
    51241 gatttggaag agatattgat tcatttgaat cacaaatcaa tcttgttcga atatggcaat
    51301 gttttgttcc aatcaaggtt gcttgaaggt aattttccgg agacatcgag attgattccg
    51361 atggaatttc cgatcgtcat caaattcaac cgcgacaaat tatacgtggc cgtcgaaaga
    51421 gcatcattgc tttcttctaa agaaggaacg tccagcatcg taaaactcaa tttgagaaac
    51481 gacaacatcg tggaaataac ttccaattcg ccagaacttg gaaaagtgat ggaaaaagta
    51541 tatccgatcg aaaaagcagt cggaatgaca ataaaaatcg cttttagttc caagtatttc
    51601 atggacgcct tgaaaacatt caattcggaa gagattttcg tcaaatttac cggagagatc
    51661 aggccgtttg ttctcgaagg cgacaacgat ccgggattga tacaattgat cctgccggtt
    51721 cgcacagaat agtttgataa aaagacagga gcaattcttg tcttttttgt tttatagcaa
    51781 agaaagaaaa agatgaaagt cattgaaaat atctttcatc cttttatgtc ggtaaaacca
    51841 taccggtata tgttataatt gtggtgcgca aaaacgaaca tacaagcgtt ccaataaaat
    51901 acgaaggatg tgataaaatg caaaatcaga tcgccggacg tcatgcggca aacaaaaaat
    51961 tggaatcgaa cattttgcaa atttcgcaag aagcaaaggc tatgaagaag aagtacgacg
    52021 atgtaattga tgctaccgtg ggaatgcttc atcacgagga aggtaacgtc ttcaagtatc
    52081 aaatcgtcga agatttgctg aaaggattga ccgatgcgga aacatatcat tatgctcccg
    52141 tgagaggaac tcctgctttc agcgaaggtg cattcgattg ggtttttggg aaaaacgccg
    52201 aaacgatcag gaaaaatttt tatcatgcgg ttatcccgac aaccggaggt agcggagcta
    52261 tcagcgatac tttttataat ttcaacgatt tcggtcaaaa agtcttgatt cccaaccatt
    52321 attggacacc ataccaaaat ttcgcctatg aggcaaattt gggattacag accttttcaa
    52381 tgtatgatga catgaagcga ttcaacgtgg ccgcattcaa ggaagcagct ctgaatttgg
    52441 cgcacgaaca aaaccgtcta agcgtattgt tgaatgatcc gtgtaacaat ccgaccggac
    52501 tttgcatgac aagggaagat tgggacaaag tcatagaggt gttgaaccaa attgcttcag
    52561 aaggcattcc agtaactttg atgcaggata ttgcttatgt cgactatcaa atgaaaccag
    52621 ttgaaatgtc ccgcgatatt tttctttctt atctcaatct gcacccggat attttgacag
    52681 tagtcatatt ttctggttcc aaaacatttt cattgtatgg attcagggtt ggagctcaaa
    52741 tcgggttgag taaaaacaaa gatgttctcg atacgttcgt aaacgtcgga gactattccg
    52801 tgagagcgag attctcaagc gtaaaccaac ccggcatgag cattatcgga aaaatattct
    52861 ctaacccgga aaacaaacaa aagttccagg acgaacttgt cattggaaga aaattattgc
    52921 gcgacagggc ggttctattc atggaagaag ccgacaaagt cgggcttgat atgttaccgt
    52981 actgcggtgg attcttcata agtgttttga ccgataacaa gaatattttc caggatttgg
    53041 caagtgatca cgtctatgtg attcacatgg acaacctcat aaggatagca atcagttcgc
    53101 ttccgcttac cgatattccc cgattggtca gaatcctgaa aaaacacgta taaaaaaagg
    53161 agtcatcgcg actccttttt tattagacca aaaagacgac tttgcccttg agcaggattg
    53221 aaataagatc cactagccaa agaatgcagg ccccgaaaat aatccacaaa ataccgatta
    53281 tgagtctccc tttggcgatg cggtagatcc catagatgat accgtcgata aacggcagag
    53341 ccaaaatcac tttgatgatc aagggcaaac cgtctatggt ccttacgtat tctttcatat
    53401 atatcatctc ctttataata aattatatca catcaaaatc ttttgaaaag cggagcggca
    53461 aaattaatca ctccattgtt ctgcctatcg cgccggctac cagacggcag ctttgcatga
    53521 gagttgagaa acgttctggt ttcaaggatt gttgaccatc gctcagagct ttctccgggt
    53581 cggggtgaac ttcgattata aggccgtcag ctccggccgc cacggcggct ctcgacatgg
    53641 attcgatgaa ttcccatctg ccggcggcgt gtgatggatc gacgattacc ggcaaatgcg
    53701 aaagtttttt caagacagga atggaactga tatccaaagt gtttctggtc gatgtttcga
    53761 atgtacggat accgcgatca catagaatca ccttatcgtt tcctccggcg aatatatatt
    53821 ccgctgacat caaccattct tctatcgtgt tggccatgcc gcgcttcaaa agaacaggct
    53881 tgtcgagttt tcctacttct tttaaaagtg aaaagttttg catgtttctt gcgccgattt
    53941 gaataatgtc gacgttttct atgaataaag gaatatcttc gatgctcatc agttcgctta
    54001 caacaggaag ggaatattct cggccgatct gacccaggat tttcaaccct ttttccccta
    54061 gtccttggaa agagtacgga ctggtacgcg gtttataagc gccagcacgc aggattttgg
    54121 caccggcagc tttaacaagc ggggcgatag attgcatttg ttccagcgat tccaccgcac
    54181 acggcccggc tagagccaca acgtttttgc ctccaatttg tactcctttg acatttatga
    54241 ttgtatcttc gggatgaaac ttccgattca cctgcttata tggttcttga actcgtaaaa
    54301 ccgtttcaat aaaatcatat acgtaaagag aattcggatc gaatttggat gtgtctccga
    54361 ttactccgaa aagggtaatt gaagtccctg tgctttcgtg gatggagaat ccctggtttt
    54421 gtaaaacgtc acgtagcaac gcgatttctt ctggtttggc tttttctttg aatttgatga
    54481 tcattttgtg cgggatcctt tcttcaataa ttcaatagct tctgtatatc cagaaaaacc
    54541 tttgccacat atcgtaccga tgcaagcggg cgaaatgact gagttttgcc gaaatggttc
    54601 gcgggaataa atatcggata tatgtacctc gacagcgggg atcggaacgg aaagcaaaca
    54661 atcgtagatg gcatatgagt aatgtgcgaa agcgcctggg ttaataacga ttccggaggc
    54721 cttagtataa ttctcctgaa tgaaatcaat gatggatcct tcgtgattcg attggaaaaa
    54781 aaacaaattg tcccccgata aagccgagta gccggaaaga taacccaaca aatcggaata
    54841 actcgctttg ccgtatatag caggctcgcg taaaccgagc aggttcagat taggcccgtt
    54901 gattatcaaa atatccataa aagcgctcct tgattagcgc tgcctccgaa gcgggatcgt
    54961 tagaaacgga ttcatatcgg atgtcagcgt atttttcata taaagaccgc ctgcgttttt
    55021 ctaaggcaag caaatcttcg ggtgttttga gcaaaggacg gccaggaaca ctttctttca
    55081 ggatgaaagt gattggacgg tcaagaaaga ctaacaagcc gttttgacgc aaaaggcgca
    55141 tcaagttgtc gttttccacc atgcctccgc cggtagaaat aaccaaccct tgattttgat
    55201 acaagccctt tgtgagttcg tattccaggt tgcggaaata gggctctcca aaagaagcga
    55261 agatgtcgga tatcgacatg cccgcggcat cggaaattag ttggtcgcag tcagcgaccg
    55321 tttttttaag tcctttggcc aacaaccctg caacggaact ttttccactt cctggaagac
    55381 cgatcaaaac caagtttgtc atttgtgcgg acaaaatgga aaaaacagag tcgatctgat
    55441 tttttgttgc aggaatgccg gtgaacaatt cgttggactt ggcagcttga gaaaccagca
    55501 tatacaaacc gttagtcgca aaaatatctt tggactttgc atcaagcaaa agatttgtat
    55561 gcaaaggatt atatatcaaa tcaattacgg aatcaagcct gtaaaacaga ttgagatcaa
    55621 tcaaaacgga actatcgttt tctggataca ttcccaaagg cgtggtgttg attatgattt
    55681 cgctgtcaac aaccttttct atttccgaaa aaaggaattc gttttcgctc ttgatccgcc
    55741 ggcaaagttt gaccgtttgc ttggcgccga gtttatgcaa aacaaacgca catgcttttg
    55801 ccgccccgcc attacccaga ataagaacct ttttttgata tggattgatt ccggcgtgaa
    55861 cgagcaactg ttcgaaacca aaaacatccg tattgtaacc tacgagtcgg ccgttgttat
    55921 ttaagaccgt attgaccgcg ccggtcgctc tagcaaccgg atcaagttca tccagcaacg
    55981 gaattacaga ttctttgtat ggtatggtga cattgagtcc ggtgaaaagt ttcgaagata
    56041 gaaaagactc gggatcggga ctttccaaaa gacggtaatc ggcatttccc aacaagcgat
    56101 ggatttccgg cgaacgactg tgggaaagcg atttgccaag cagaccatac attatttggc
    56161 aaccgacctt cctattcgtg acaaaagcaa taaatccaat atagtgaagg aggcgacggc
    56221 agatataaca gggattacac gcggaacaat cgacgggtcg tggcggccgg aaacttctat
    56281 ttcggaattt gtgccgcttg aaagatcaac ggttttctgt tccttggaaa tagatggagt
    56341 cggtttaacg gcaacccgaa acacaagcgg catgccggtg gaaatgccac cgacaattcc
    56401 gccgttgtgg tttgtggtgg taatgatttt tccgtcctgt attcggtatg ggtcgttggc
    56461 ttcggatccc aatttttccg taatcgcaaa gccggatcca aattcgacgc ccttgattcc
    56521 cggtatggag aacagattac ttgacaggac gctttcgcac gacgcgaata acggttcgcc
    56581 gattccaaca ggcaatccga caatcacggt ttccacgatt ccaccgaccg aatccccgga
    56641 ttttttggcg ttttcgattt cttgtttcat taattctgaa acgcgatcgg aaatgacggg
    56701 aaaaccgcta gttttcatct tttctaaaaa ttcattattt aacatatcat ttgcaaaaag
    56761 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttgattt tgctgttttt
    56881 aaaacataag atccgatggc gtttacatca acatacgtga gagctttggc gcgattcttt
    56941 ttgtctttca tcacataatc ccaaagcaac tcgttcgaag cttcgtctgc ggttggaaga
    57001 ccttgcgatc gaagcgcgtt ttccaacctt tgtttgatgg tggcgtttga cactatgcgc
    57061 atcattccaa tagcgacggc ctcgccgtgc aagtattttt cgtatccata gaaagcttcg
    57121 attgcgtggc cgaaagtgtg gccgaaattc aaagcctgtc tggaaccggt atcgaattca
    57181 tcctgttcga caaaacgttt tttgatgttt atcgatctag ttatcaaaaa attcagctcg
    57241 gaagggagta ccgggttttc taatttttca aacaacaacg aatcggcgac catcccgtat
    57301 tttatcattt cgcccaaacc gtttcggaat tgtctttccg gcaacgttga taacaaagaa
    57361 ggatcgacca aaaccatttt gggcggataa aaacacccga caatgttttt tgcttgtttt
    57421 gtgttgactg caacttttcc gccgacgctg gaatcgattt gtgctagaag ggaagtggga
    57481 atttgaacat actctatacc gcgcagataa gtagctgcta caaatccggc aaggtcacct
    57541 gtgactccgc cgccaagagc aacaagcaaa gcgtcgcggg aaacatggtt tgccgaaagc
    57601 aaaccgacga tcctttcgta ttgttgaagg gatttgcttg tttctccctg agggaaaata
    57661 attttgaggc agccggggaa tttgtttacg acggtatcta cataagaaac cggaatacca
    57721 tcgtcggata tgaccacaac ctggcgagtc acatcccaaa attcgtccaa acgagaaaga
    57781 atgccgtttt caacgtgaat gggataagaa aagaaacggg atttgattat aatagtttcc
    57841 atttattctt cctcctttat aaaatcgatt ttcaaaccca aagaagccag gtctcggaaa
    57901 aagcggggat aggatttatt taccgcttcg gcgtttttga tcaaaaccgg attttggcag
    57961 c
//
LOCUS       DAOPJL010000004        44819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_868_length_44819_cov_8.125324, whole genome shotgun sequence.
ACCESSION   DAOPJL010000004 DAOPJL010000000
VERSION     DAOPJL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 44819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44819
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_868_length_44819_cov_8.125324"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="PLP02_01150"
     CDS             complement(<1..131)
                     /locus_tag="PLP02_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPF42107.1"
                     /translation="MIYHALAIDGPAGAGKSTVAKLLAKRLGYTYVDTGAMYRATTL"
     gene            complement(208..930)
                     /locus_tag="PLP02_01155"
     CDS             complement(208..930)
                     /locus_tag="PLP02_01155"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010284332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPF42108.1"
                     /translation="MERLQKVLQAAGVASRRKAEEMIAEGKVSVNGVITTQMGVMVAD
                     NDRIFVGGKELKREKKVYYALNKPTGYISTTSDEHGRPAVIDLVQGKERVFPVGRLDY
                     DASGIILLTNDGDFMNQLIHPRYKVEKEYHLKVKGLVRKETSAKLARGVDLGDFVSQP
                     AKVFDVKYDDAKENTYLKIIVTEGKYHQVKRMLEAVGHPVMKLHRYRMGCVTTEGIPL
                     GGYRLLKLHEIRKLWNLSNNGK"
     gene            complement(994..2361)
                     /locus_tag="PLP02_01160"
     CDS             complement(994..2361)
                     /locus_tag="PLP02_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPF42109.1"
                     /translation="MWLVTLEATFRPGLLAIMLAMLFFSAFFSMAEMAYSAVGKVRIK
                     TLVEDNARGSKKALWIVENFDKTLTTLLVGNNLSNVALTSVSALFFAKLFLDLTNADT
                     VVTIINTVAITIIVLIFGEILPKSVAKTHPEKICLLFSGLVYFLIKIMTPLTYPFRLL
                     NHRVIQQIETENKLTVTESELETIIDTMEEEGSIDEDEADMLQNVLDLSEITVEEIMT
                     PRVDMIALDVSTPVSEIRDTFFKSKFSRVPVYDGNSDNIIGILSERDFYTKLIKGHSI
                     NIKKLLRKPIFVPSTTKVDALIELMQAENSHLTIVVDEYGGVDGLVTMEDALEELVGE
                     IYDEHDDVKEAIVKKAPNEYVVNADYDLKDLFEDLELGEPPVSDATSIGGWLFEKFQD
                     IPEVGERIEYELSVNQVYDDLSELVSEDHELLTFEILAISKRRIKSVLLTIELLPKET
                     EAEES"
     gene            complement(2406..2966)
                     /gene="efp"
                     /locus_tag="PLP02_01165"
     CDS             complement(2406..2966)
                     /gene="efp"
                     /locus_tag="PLP02_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPF42110.1"
                     /translation="MVSTNDFKTGLTIEYEGNLYQVIEFQHVKPGKGGAFVRTKLRNL
                     RSGTILDKTFNAGVNVNQALIDKSQMQYIYANGDTHVFMNNETFEQIELHKSQIENEL
                     YYLTEGMSINIISFEHEILGLELPDKVILEVVETAGGAKGNTASNATKEALTNTGLRL
                     LVPLFIEPGEKIVVSTSTGKYDTRAK"
     gene            3092..3490
                     /locus_tag="PLP02_01170"
     CDS             3092..3490
                     /locus_tag="PLP02_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPF42111.1"
                     /translation="MQFLSVTDWIWPISIGLLVGILISARKRHNYNDIVYLEADEFRQ
                     NMRKGQLIDIRNEEAFKKERINGSRNFPGRSVFSNLNRLRKDQPVFLYSDTDSFKVRQ
                     TAKKFIRKGFQPVYVLKGGLTVWSFPLKND"
     gene            complement(3519..4427)
                     /gene="miaA"
                     /locus_tag="PLP02_01175"
     CDS             complement(3519..4427)
                     /gene="miaA"
                     /locus_tag="PLP02_01175"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPF42112.1"
                     /translation="MKPILAIVGPTATGKSDLAVKLAKKYDGEVISADAFQLYKRMDI
                     GTAKIKKTEMQGVRHHLIDILEPNESFSASDYQKMVREKIELVSKEGRMPIVVGGSGF
                     YIKAALYEYEFPGEKRNDELTAEYSDYDNSELYGILEKQDPQLAKTIHPNNRRRILRS
                     LEIVGTGSNEFRKKGSIPFYDNILLIGLRMDRKELYKQIDERVDRMIENGLVEEAKAL
                     FEAGITGQSISAIGYKELFMFFRNEIRNEEAFDLIKRNSRRYAKRQLTWFKNQMDVTW
                     FDVTNDNRITLYDEIVNYIEKKGEEI"
     gene            complement(4424..6133)
                     /gene="mutL"
                     /locus_tag="PLP02_01180"
     CDS             complement(4424..6133)
                     /gene="mutL"
                     /locus_tag="PLP02_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPF42113.1"
                     /translation="MGHIIQLDARLQNMIAAGEVVERIVNVVKELIENSLDAGATRIQ
                     ISLKDSGFAMVQVSDNGSGMDPEDSLLAFSRHATSKIKNEYDLFHITSLGFRGEALPS
                     IASVSRVEMETSDKKVGTRIVYEDGRLLVNEETATRDGTTITVTRLFFNTPARFKYLK
                     TETVELRLIVDFVDKMAFAHPDVAFRLENNGRMLLQTSGNNSETDIFAVVYGLEVAKS
                     VKRFSGKNRDYSIEGYFANPIHNRSGRQSVILIVNGRPIRDFILTREIVECFIGYLPK
                     DKYPIILLNIAVDPQLIDVNIHPTKAEVKFSELETLRKLISETLKEAIGKTKVIQTAS
                     DKSDTQIKMGFGTSYPNKEPEEGLFVKEEEEIPKEDKASNEFPDFEYVGQYGGTYLLF
                     QNEAGLYLVDQHAAAERIRYEKYLNLMLAPNKETYQLLLPMELSLSNTLTEMIGKDTI
                     DKAKENGLVIRKKDLSTILLETVPSWYPKGHEADFAETVVSSLIEEGKTDKKTLVDET
                     AKLLACKRSLKANHRLSREEAERLVGDLSKCNRPYTCPHGRPIIVLIGEYDIEKWFYR
                     VAS"
     gene            complement(6148..8742)
                     /gene="mutS"
                     /locus_tag="PLP02_01185"
     CDS             complement(6148..8742)
                     /gene="mutS"
                     /locus_tag="PLP02_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPF42114.1"
                     /translation="MDHNSLDRSNYTPMMQQYLEIKKDYSDFIVFFRLGDFYEMFFTD
                     AIVASKELEIVLTGRDAGMEDKVPMCGVPHHAANFYIEKLIDKGFKVAIVEQIEDPQL
                     AKGLVKRDVIKLITPGTIVEEGAVEEKSNNFIAAISETKTQFLLAYSDLTTGENHIIT
                     LPNDIDILMNEILNLNVRELVLGSDFNQSRIREYLNSYALIVSISDNLELPAYCKSLV
                     QDIYDKELLQTFARLMNYLYSSQRRELMHMQKVQVFESSKYLHIDNNSRRNLELTETY
                     RSLSRKGTLFWLLDKCQCAMGSRMLKSNIMRPLVDKEKIEERYRLVEAMSDNFIIREE
                     IRNLLKEVYDLERIVGRLSFGNANGKDLVNLARSLKSVPGIKSRLLSLEDDLANRLLK
                     GMEDFTQLEDLIGQAIVEDPPFSVKEGGIIKAGYSSELDEIKYRATHGKEWLRDFEEK
                     ERERTGIKKLRIGYNRVFGYYIEVTKGQIDQIKPEFGYDRKQTLVNCERYITPELKEK
                     ESMILGSMDKSIQMEYELFCFVREQAKGMTDNLQKLAKSLAEVDMLTAFAIISIENGY
                     VRPVLSNEKKIMIVEGRHPVVERTVARGSFVSNDLNMDSDTSILLITGPNMSGKSTYM
                     RQLALIVIMAQIGCFVPAKSALLPVFDRIFTRIGASDDLSTGKSTFMVEMMEVNYALQ
                     NATENSLILFDEIGRGTATYDGMALAQAIIEYTHHKIACKVLFSTHYHELTYLEDTLK
                     SLHNIHVMAKEDKGNIVFLHKVADGPTDRSYGIHVGKLAKLPTQLVRRAQTILEDLEK
                     NHGYNVIKPQTVDLFNFETASLEEEREKSEYRPIIDQIKEIEINELTPLKAMNILASI
                     IEEIKKIN"
     gene            complement(8772..10172)
                     /locus_tag="PLP02_01190"
     CDS             complement(8772..10172)
                     /locus_tag="PLP02_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42115.1"
                     /translation="MSRFKANEDYFAFAKTLSVIPTEDLLELLLQHKIMIPTYIHRFV
                     LRETIYPKVFQTKLYESYTDELRYRLRGFNDYSVYLLEKLIYDYNLDFDAAKYKQLLF
                     DILFLNRELYGLKNAFFDDLEKLKYKYAVDLEKINYGDFIGMMERVIYEPNGYLDGVS
                     LKILKDVFVHSCTLGDLRGLGEKYGVKVPRRINKSSLIEILAARFRLTEQEATLLQDK
                     SVLELEIYAKEKGFQISIDLKKSDMVEYIIYALNLYHQEIAKDVHDYNIPLESEFDSV
                     KIDAIEFQANDQDIPVIEAEPAEKEIMRSIEVDEEPAETISVAKEETILKPEEEPVKE
                     EETIELPEEEQKMPEEIKKTEEIVTKKPVISPEPEIIPEKKEEPIVAPVLEKTATEAE
                     KPQSEIIEFSDEEKDLLDEKINLIIKKYHQKKRKKRFWTIFLITLFAAIIIIGAYTYV
                     YHFTLNPGHWPFGLFE"
     gene            complement(10321..11754)
                     /gene="miaB"
                     /locus_tag="PLP02_01195"
     CDS             complement(10321..11754)
                     /gene="miaB"
                     /locus_tag="PLP02_01195"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPF42116.1"
                     /translation="MIKPELKSARKRTKGPVEIRQYKPSSGIDCIGKDKKYFIETYGC
                     QANEADSERMAGLLSMVGYEAADCIERADIVILNTCAIRENAEKRVYGEIGRIMKYKA
                     ANPDLIVALGGCMPQEETTVEKILKTKNRVDILFGTHNIERLPEMIKERIESGKMVVE
                     VLSEEGDIVEKIPSVRNHPFKAWVDITYGCDEFCTYCIVPYTRGKERSRQIEDIVEEV
                     SQLAKKGYREVTLLGQNVNSYGLDYGDPEITFAALLDKLNKLPIERIRFMTSHPKDFT
                     DELIDILAKGGNLMPFIHLPVQSGSNAILSKMNRKYTKEQYLELVNKIKTKIPGISLT
                     TDIIVGFPGETEADFQETLDLVKKAGFEGAYTFIYSKRAGTPAASMPDDVLAETKRDR
                     LKRLNELVNRGYASGTRRFLDTTTEVLVEGKSKTDATKYCGYNPNNKLVNFPGNPGMV
                     GKTVSVNITKANTWSLYGEVTDKEANK"
     gene            complement(11778..12536)
                     /locus_tag="PLP02_01200"
     CDS             complement(11778..12536)
                     /locus_tag="PLP02_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPF42117.1"
                     /translation="MRVLFIGDVYMEKGREAFDRFFSTAKQLYRPNFIVVNGENIANG
                     NGLSEEIYKDYMKRGVNVFTLGNHAYSRKDSGEVLNLDYVVRPANYGPGAAGKGFVTC
                     RVNDKTITVVNLLGRVFMNDPIDSPFIVADEVIQKCDSDYIIIDFHAEATSEKYALAN
                     YLDGRVSALVGTHTHVTTADECVFPKGMLYITDIGMTGVKYSIIGGDIGQGLRKFITG
                     IPERTRPEKNGPLQFSAVLLDLEQKRIERVNYAE"
     gene            complement(12591..14150)
                     /gene="rny"
                     /locus_tag="PLP02_01205"
     CDS             complement(12591..14150)
                     /gene="rny"
                     /locus_tag="PLP02_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPF42118.1"
                     /translation="MPWPEIISSLLGLIVGGIIGFLTRVSVVEKGFQTAKNKAQNIID
                     EANSQAEKIKKEKLLEAKQEIYNLNSENDKLIKEKKALISELENKVNQREETMDRRSA
                     NLDKRELNLDRKEEILDEKKVALEEKNSKLDEIIQEENKKLLDIANLSEQQAKEIIMA
                     RVESDMAQEIGRYLREEEEKARLDAQKNAKEILSQAIQKYAQDVTAESTVSVVNLPND
                     EMKGRIIGREGRNIRTIEAMTGVDLIIDDTPEAVVLSGFDPVRREIAKRSIETLISDG
                     RIHPARIEEIVEKTRVEVDQLIRDMGDKAVFETGIGKVHPDLVKLIGRLHFRTSYGQN
                     ALQHSIETAYLAGKMAVELGENEVIAKRAGLLHDIGKAVDHEIEGSHVDIGVMLANKY
                     HETKPIIDAIASHHGDKPAETVIGVLVAAADALSAARPGARSESLENYINRLTQLEEI
                     STSVEGVDQAYAIQAGREIRVIVKPNEIDDAKTFVVAREIKQQIEEKLSYPGTIKITV
                     IRETRAQDIAK"
     gene            complement(14318..15313)
                     /gene="recA"
                     /locus_tag="PLP02_01210"
     CDS             complement(14318..15313)
                     /gene="recA"
                     /locus_tag="PLP02_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPF42119.1"
                     /translation="MADEKRKKILEVALKEIEKEYGKGAVMILGERQDDMSIEAVSTG
                     SLALDSALGVGGYPRGRIVEIYGPESSGKTTFALHAIAEVQKNGGYAAFIDAEHALDA
                     VYAKNLGVDIDSLILSQPDTGEQGLEIAEALVRSGAVDIMVVDSVAALVPEAEIRGEM
                     GDSHVGLQARLMSQAMRKLSGVLSKSQCIAIFINQIREKVGVMFGNPETTPGGRALKF
                     YASVRLEIRRGEQIKEGNEFIGNQAKVKVVKNKVAAPFKTCIVDIMFGKGISKTGELV
                     DLAADKDIIQKSGAWYSYKDQKIGQGRENVKKYLEENPDVYEEIRQLVKKELNLK"
     gene            complement(15381..15950)
                     /gene="pgsA"
                     /locus_tag="PLP02_01215"
     CDS             complement(15381..15950)
                     /gene="pgsA"
                     /locus_tag="PLP02_01215"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPF42120.1"
                     /translation="MNLPNRLTIVRMIMVPLLIVVYLLKGTLGNMTYWIMGILFVLAS
                     ITDYFDGKIARKRNMVTTFGKFMDPLADKLLVLSALIVLADNYRIVPNMWLPFWVPVI
                     VLARELIVTSIRLVAVGNGKIIAASQLGKFKTASTMAAITWYLFMMPYDLLWANITGW
                     VIMGIAILMTVISGVDYFIKNRKLIFETF"
     gene            complement(16056..16784)
                     /locus_tag="PLP02_01220"
     CDS             complement(16056..16784)
                     /locus_tag="PLP02_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017444581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF42121.1"
                     /translation="MGRAHEVRKAAMEKTSMMKSKLYSKFGKEIYMAAKTGGTDPDGN
                     LVLKRTIERAKQNQVPGDVIKRNIDKAKGGAGEDYTPARYEGFGPGGATVIVECLTDN
                     VNRTFGEVRSCFTKTGGKLGVSGSVTYLYTYSSLISVEGISEEEALEAILEAGIDIAD
                     IAEEDGIVMITGAPADLDAIKDALDASKKELNYLEDTVTYIPLESITLTPEDLGKFQR
                     FLNMTNDLDDVQEIYHNVNIPEEE"
     gene            complement(16803..17666)
                     /locus_tag="PLP02_01225"
     CDS             complement(16803..17666)
                     /locus_tag="PLP02_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HPF42122.1"
                     /translation="MTLQIGKTADLKVSRITDIAYILTNEKEEIFLHKKETGSELKPG
                     DIVHAYIYPDSYGRICASAFCPFVELGTASFLKVKGVNYEYGVFLDNNQVKDLLLSKD
                     DLPFDLDLWPQAGDSVFAVMTEKKGQLFAHIPGRKQITGFFKEHRELDLEKVIDAWVM
                     FILPEGLVAFASEGQEIFIHKNNLRDNFRIGEQIAPRIVKKNSDNEYVASLIERKESM
                     IDPDARTILAYLENHDGYMRYSDKSLPGEIESAFGMSKSAFKRAIGHLYREGRIVLYP
                     DHTEMACKSKK"
     gene            complement(17663..17794)
                     /locus_tag="PLP02_01230"
     CDS             complement(17663..17794)
                     /locus_tag="PLP02_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42123.1"
                     /translation="MLKEKTFDISNFQQKGSGNDRRSLSINRGRSAFLLAMVPLGLI"
     gene            complement(17911..19365)
                     /locus_tag="PLP02_01235"
     CDS             complement(17911..19365)
                     /locus_tag="PLP02_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF42124.1"
                     /translation="MDWYWYLVIGLCSIVTILLAVAIIRTFLIKVEPVAKPDFSEFTP
                     KMQEYAENLSRMLRYKTIASISGTENEFIGMQSEMEKMFPELHALAKKKVFPNNSLLF
                     LWKGTGKKDPIVLMAHQDVVPADENVWSYPPFEGTIENGKIYGRGSFDTKVTVFAIMQ
                     AINELIKEGFVPEGDVYLAFASDEEVSGHGAKDTAEYLKQIGVKPAFVLDEGGAVVRD
                     SLPSIKIPMAFVGVVEKGYVNMMFKAKSEGGHSSTPPRNTPIARLAMFVNDVERHFPL
                     KTKMIPSVRAIFANAAPGMSFPYRFLFGNLWLFKGLMTGLLPKISPFGRALLSTTIAF
                     TMSSGSDAYNVIPSEANVIANIRIHPIQNVDDTYRVLSKIAAKYDIETIILESEDASK
                     IVDTRSKAYQSIERQIRKNFPEALVSPYIITGATDCRHYALITDDNLRFSPVIISDED
                     LGKMHGKDETLEIESLYRAVNFYKSLIRDYSVDK"
     gene            complement(19386..19934)
                     /locus_tag="PLP02_01240"
     CDS             complement(19386..19934)
                     /locus_tag="PLP02_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPF42125.1"
                     /translation="MRIFLHICCAPCSVMVIKKLREQNHDVTGYWYNPNIHPYMEYKA
                     RKEALEGYATDIGLDLVENDVYGLRMFVEHVAKDLNNRCMFCYEERIRKTAIEAKKRG
                     FDAFSSTLLISPYQKHALLVEIADRIGKEEGIPFYYEDFRPFFYEGRKIAKELPLYMQ
                     KYCGCVFSEEERYLGEKKKKNN"
     gene            complement(19951..21654)
                     /locus_tag="PLP02_01245"
     CDS             complement(19951..21654)
                     /locus_tag="PLP02_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF42126.1"
                     /translation="MTTNLSPKETNDLGDKYFYGKGVPQNIELAYTYYKQTADEGNPV
                     GLYNVGRYFIEKQEYKQALSYLQKSLASGYSKAHLVLADMALKGKGMLKSKKKAFRYT
                     MEGAKLQDTDAYNQLAGYYLKGIGCEKDIRKAREYYQKSADLKNPEGMYRLALMMLET
                     GKGQKNPEEAMKWLDEAVEANHKEAIDAIYSLYEKPHPYFKKRSQAFRKEMMFYYLEK
                     KARIGDEKALEKVAQDYLEGTMITPENPEKAAEYFRLLQAYDNVIGHYGLGVCLLKGL
                     GMGKNVESGKAELLKAANLGNVKAMIALGDACRLQSGSDIDMEEAKRWYYEAAKQNDP
                     EALVNLSLLHYRKDITNSSEELAFKYMENALKKGTPKAHYWLGIYYEKGVGTPINISK
                     AIKHYQKALDAGVSASAFKYATCLYDNAKNKQKKDQKMYAKALDLFREYVKYPQHLES
                     NVAMACSQIAIMYKEGLGTQKSLRRSRYWFELAASGASGIAMLEMYYLLRATEIVPAM
                     KWLEKSANDAKEPAALYELGILYLNGAEGIRTDLARARQYLELSARSNYKPALDRLKM
                     L"
     gene            complement(21656..22603)
                     /locus_tag="PLP02_01250"
     CDS             complement(21656..22603)
                     /locus_tag="PLP02_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF42127.1"
                     /translation="MENSELEARALAKYDAKQYSEALSLYRKLSKMGSKVAWHHLGLM
                     YFYGYGEKQNYERAYECFEKAAVELYPEAIYMMGYCYEEGLGVMKNLQKAYDYYVTAA
                     HKDVADALLAEARFFEEGKIVQKSKARALEIYVELAKKDNPFAMYKIGLAYFDGDGVR
                     KSLESAYSWFNKALSKGSIDAMNQFRLIGTKSVSDIRETKTLYTAAKEYYMSDNPEKS
                     IIYFEITAREGITEGYRYLAEAYEKGKGVEKSSKKSYGYLLKAADAGDVTAMIMLGHR
                     NENGDGVPSSYIKAGEWYEKAAKLGSEEAKSELFGLRGY"
     gene            complement(22637..24442)
                     /locus_tag="PLP02_01255"
     CDS             complement(22637..24442)
                     /locus_tag="PLP02_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPF42128.1"
                     /translation="MEMILPRPKSIEIIEGEYVFPQTTVIACDILTESIGKVASKYLN
                     IENASKEADLEFAYEPGLKDEEYILTIGKKKSQIRYTKYSGALRGLSTYIQLQREGKV
                     RCQRIDDWPDLGIRGFLLDISRDKIPTMKTLKEVIDMLFISKMNHFELYVEGFPVYLK
                     SFPNLPYEDPLTPKEMDELESYCLARGIDFVRNINTFGHMTAWLGLDFFHNMAECEEG
                     YVNYGYPFPASTLNPLDPRSIELVKKMIDDACGNSQSAFFNVNGDEPFELGLGKSKEK
                     CAEKGRGKVYLTYMEKVMNRVREKGKRPIIWGDVFNEDPSLYELVGNDVVVCDWGYDS
                     EHDFETPSIRMEKAGKDFLLAPGTSSWNSFSGRYEDMRDSIRNAAMAAKSHMGLGILL
                     TDWGDYSHPQSLSFSLPSLLFAATASWGEAEDIEKTEKWLDQNVFNDKSNGFADKIIE
                     LSKYHMLEDFNIPNGTMTFASWMFVDPDESHPLELKYAIWQDALTKQKISPDNAEKIL
                     SLAKRIEDSVFCNEECLVAKEIVQTCRLIEIGVSMNQIANGIKSDKKQTLALIESVMK
                     TYGNVWLERNREPGLKKSLLHLEIIAAFLRNNLLV"
     gene            complement(24446..27646)
                     /locus_tag="PLP02_01260"
     CDS             complement(24446..27646)
                     /locus_tag="PLP02_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPF42129.1"
                     /translation="MTIDLYNLTAMQKKAVMHEGENILVAAGAGSGKTKVLSLRVMRK
                     LTDGIDIDKLVILTFTRLAAEEMKARIRKSVSASKDLKPIESRIENATISTFDAFALS
                     IVRKYHYMLNLPSKIDIADEAMLEALREKCLNETLDEFYIMKDPGFVRVVERLFDRGD
                     DKLKEAINVASFGLETLPDYHRYLDNYENDHFSSESIGNILKEFNDYIGNRMIASTAA
                     GSSVFYDTSNPVINEYFLRMKNFYHRIKEANTLEEKIDFLRNESFPRSLTKSSKIEDD
                     TLDEIKSAHSRAKTIYDGIAKTVKSLQASSKETLIEDIQETKETVLTILRLADAFLLK
                     MENAMREKEMYHYGDIMRFAIKLFEDNPELRDRLSGEINEIMVDEYQDTNDIQEYLLQ
                     LISRNNLFMVGDIKQSIYGFRNANPGIFLAKYHAYEKGSGGILIDLRENFRSRPEVLE
                     YINDFFMPVMDEEIGGIDYFHNQSMIYAQKNYEKHSILAAGAKKLVYIPELRPDDEDK
                     TKTEALAVACDIFGKLKNRETTLDRETMTERSLKYGDIAVIADRKTGFDIIAKTFSDM
                     GIPVKVYTEESFIMTEEIMMVWQFMQAAFCLQNNDFLDHSLARSVVSLARSFAYRIKD
                     DDIVCFLKCEGLENIDGFIKLKDNKAFAKLAHDIKNVASVINIIPLPEIVSMICKVTG
                     AYESIIRLKDPEACEKRLDYLYMKALELQNIGFVGFLDYLNSVGLKEEADIAYENVAA
                     DTSDMVKVLSMHKSKGLEYPVCYYVGLDKKFNFTENKNLFVFDRKYGLSTKTWSDGFK
                     PNIVGLLLLRRARKEYVSERIRLLYVALTRSREQQIIVLPEINKEEAEASFMDNGCLD
                     SYTRESINKYSRLFSLLPHIPEKKEEALINIPEMAEKPVKVFAAKPVIYLNLTWEKKD
                     LIGERFSKESAGILLKEEEKNALAYGNLLHEAMEYVDFNHPENSLENIPDNAKDRIIK
                     LLQKEPFDKIRKMRIYREYPVYEEIDGIAKNGVIDLLALSDKEAIVVDYKSGDIDKSE
                     YTEQIFGYANIIRKMTNKPTSAYLCSLWTGAIRKVD"
     gene            complement(27636..29948)
                     /locus_tag="PLP02_01265"
     CDS             complement(27636..29948)
                     /locus_tag="PLP02_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPF42130.1"
                     /translation="MMDKRTLTDIGLSCKDLVLCAFSDRERIVSEIRDEYGLFPARIK
                     DIGYFEPIFSKDWLLPVSRHFGISPDLAEGLKEAIAHIDIKKEYVGLALLVLEIRKFL
                     EENNLISKRLIPSDDIKRIIVVNKIALDCLPFNVPFEVVYLGKECHNDIDLFESADDI
                     EKCENCYQCITKMLASGIEPGTIRVLGGSKEDVYRLKRKLEMAEMPFYDTRGRTLSGH
                     PIAQEFVMLSKTVGTWSAFQTIKEKPGNSVINCLADLLNDYGCENLDENPELLHFETE
                     KRKYRETKMTDAIVFANIDHIGYDENTFNLILSADMDALLAIKGNRYLDDNVLEAIGM
                     KTAYKKTEERFAHLEQIFRRVPNLTLFMSKLTSGIETRKPEFALGNRKIRESAGPIIP
                     GMSFSRRHDILEYAKLRDKETTYGNKSALSGVYEKEFVGKIIGYDPKFSGLRKHTIEK
                     IKEKTIGISPSGMNVFFGCPYRFLIGNILRLEPEKENIKAFFGSLAHAVIANGTIDLK
                     DMEKFPDDLSARKEIFMECFRKQIEKTLSMLDIFTGQSEFELSGREKNVSLDPENLSG
                     YVIHGKIDRVMTKSIEGKDYYVIIDFKSGNNSFKEDDFEKGTDVQLPFYLYLENDSAS
                     SERKIPFGFFYQKILPSLYSKSEGKDSYKKKMELDGRIISDMEIIESFSDISWIRGVS
                     LNASGGIRDGKRTVGTEYMDNLMKTMEKHINAMTEAIDEGKFTIIPQMKKVAYGLSAA
                     CRYCQNRPLCHLEAAGKDELLESEGSDDDD"
     gene            complement(30092..30520)
                     /locus_tag="PLP02_01270"
     CDS             complement(30092..30520)
                     /locus_tag="PLP02_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPF42131.1"
                     /translation="MNSIIKKDNNVSILDDFFDDFFTVRPPMRQAAQCMRTDIKEVEN
                     GYELVIDVPGFGKDDIKISLEKGYLNIEAKIEEDKENKDAKGHFIRRERFLGSSARSF
                     YVGDDIAEKDIRASYDKGILTVFIPKEGTNVKEKKYISIE"
     gene            complement(30715..31926)
                     /locus_tag="PLP02_01275"
     CDS             complement(30715..31926)
                     /locus_tag="PLP02_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42132.1"
                     /translation="MKKITHVLVFFFLIIASFVFYTVKVSASSPVNLYFFYDITCGHC
                     QEEDAYLSLLESEYPDIVVNRYELSESDYNLALYDSVRETFGKSMSLTPLLVIGGVAL
                     TGFSEQTETDIVSLIERYTTATDYVDVTEKIINGETVDRDDIEYLRFSWGDYVYIPIF
                     GNVSVDSFSLIVAAIVIGLVDGFNPCAMWILLFLITLLVELPDKRRRWTLGIAFLLTS
                     GIFYFLIMTTWLKIGISIAAIKWVRIAIASFALAFGIYNITRFVISLKHKETGCDVTN
                     VKMKRKFQDMVRSSVAKKNILMAIFGIMLVAIAVNFLELACSAGLPLLFTQILAYRSL
                     PYAIYLMYIGIYVFFFLVDDIAVFVISMITLEVKGISNRFGKVSQLVGGAIMIAIGIL
                     LMFFPTIIMLS"
     gene            complement(31923..32186)
                     /locus_tag="PLP02_01280"
     CDS             complement(31923..32186)
                     /locus_tag="PLP02_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPF42133.1"
                     /translation="MQIKMIGAMWCPSCLIMRSRIRKLIKIHPEIALVEYDFDMDREK
                     IPEFSDAKILPIIIFEKDGRETFRFVGEKSVSELGKFMEENPK"
     gene            complement(32177..33295)
                     /locus_tag="PLP02_01285"
     CDS             complement(32177..33295)
                     /locus_tag="PLP02_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPF42134.1"
                     /translation="MIYLDYSATTPPCKKAIVCFERESERRYGNPNSLHAFGMEARLE
                     IESSTKKIKKILGNENCEIIYTSGATEANNMAIMGTAIKKQKQGKHVITTSEEHPSVT
                     ACFSRLVKMGYEVDIIEAKKDGRIDPEELESLVRPDTILVSVVNVNSEIGSVNDIERI
                     GNILKKYSQVTFHSDMTQAIGKIRLPKYYPDLFTFSGHKIYGLKGIGCLVRNDKTVLE
                     PILYGGASVSAYRPGTPPEPLIVSFAAALEDAYSKFLLKEKKIAKLNRILIEGLESLD
                     GVLLNSPDDAIGQIINFSFPLLKAPVLQKRLSDRGIFVSTQSACCFEKTYSRLINLLF
                     GDMDRAESSIRVSISHLTSDRDIRTFLDVLAMEIESCR"
     gene            complement(33317..34720)
                     /gene="lpdA"
                     /locus_tag="PLP02_01290"
     CDS             complement(33317..34720)
                     /gene="lpdA"
                     /locus_tag="PLP02_01290"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPF42135.1"
                     /translation="METRKTDLLIIGAGPGGYVSAIYAAKKGLDVTLIEKRFLGGTCL
                     NVGCIPTKSLVKSADVFTEAKEGEKYGVVAKEVTIDLENAVARKDFVVKNLTDGIGVL
                     LEKNGVKVINGTASFLDDSRVLVKSEDTETVFSAKDIIIATGSKTKHLPINIETPEIY
                     LDSEGLLKNTKLPETLTVIGGGIIGMEFAFAYGRCGVKVEVIEFLPSILPMVDRDLSM
                     RLLRYAKMSNIHITTSAKVTEVKKSESGKALVCFETNGEMRSVEADLVLEAVGRGPET
                     NMLGLENTSIKIGKRGEITVDDRMKTSVESIYAIGDVNNKMQLAHVASHQGMIAVENI
                     LGKDAKMDYTHVPSVIFTSPQIATIGKSEAELQAEGTEYRVVRVPYSANGKALISDSQ
                     AGFIKLLAKSEGNELYGAQIFGNDAEHLIASLDVAMTNNLSVEDIKKTIFAHPTISEI
                     IHEAYLGLAKEAIHFLN"
     gene            34889..35326
                     /locus_tag="PLP02_01295"
     CDS             34889..35326
                     /locus_tag="PLP02_01295"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPF42136.1"
                     /translation="MDYKDIKFKSGDSNEYSLNDFHGTGLVLYFYPKDNTPGCTTEAI
                     QFTSLKDDFAKKGYKLVGVSRDSVASHCNFTEKHNLGITLLSDPDGILAEAFGVLKDK
                     TMFGRQYKGIVRSTFIINKEGDLIKEYRNVSPFGHAVNVLADL"
     gene            35436..37562
                     /locus_tag="PLP02_01300"
     CDS             35436..37562
                     /locus_tag="PLP02_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF42137.1"
                     /translation="MNNQEPESYLDDPKVLDKFGRIINEEVKSGKIDPVIGREDEIRR
                     IIKILSRKTKNNPVLIGEPGVGKTAIVEGLARRIVDKDVPLGLQNKSIYELDLASLLA
                     GAKYRGEFEERLKAVLKKIKDSNGEIILFIDEIHMIVGAGRTEGAMDASNMLKPMLAR
                     GELHCIGATTLNEYRKYIEKDNALERRFQKVLIDEPTVEDTISIIRGLKDRFEAHHGV
                     HISDNAIIAAATLSNRYISDRFLPDKAIDLIDEACASIRMEIDSMPQELDTIKRKIMQ
                     LEIEKRALEKESDALSKDRLVKLESELAKERRSEDEITKEWEKEKHELQKVKEMKAKL
                     ENYKSELQNALNQSDYSKAAELQYSKIPELEKKIEEAKSTDANKMISEVVTEEDIAAI
                     VANWTKIPVAKLLQGDRDKLLHLDEALKQRVIGQDEAISLISDAIIRQRAGVRNENRP
                     IGSFMFLGPTGVGKTEVAKALAEQLFSSENHIVRIDMSEYMESFSVSRLIGAPPGYVG
                     YDEGGQLTEAIRRKPYSIVLFDEFEKANPEVFNILLQILDDGRLTDNQGRTVDFKNTI
                     IIMTSNLGSEYLIRGDQSGKDKVMDLVKHSFKPEFINRIDEIVIFNPLSYDVQVKIVD
                     KMLQELSLRLKEENIYVGFDSSVNKSVIDQAYDPEYGARPLKRYIQRYVETAIAKSII
                     SDLIVPGKNYLLLYDKDTGYELKPAQ"
     gene            complement(37624..39414)
                     /locus_tag="PLP02_01305"
     CDS             complement(37624..39414)
                     /locus_tag="PLP02_01305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPF42138.1"
                     /translation="MPKFRKYYTPLFSMKDFRDPMIAYSDDLIQFANDAAVKLFRCGS
                     EEALVGRKVTELIAPKYVSVYREQVDNVKNNMHSKPRTGFDLMRLDGTIFYAEVFSAI
                     SNDKPLQICSFIFERETAQDSERTMMLEKNRYDNIIEATNTATWELDVKSGKISVNQK
                     WLDMLGYSREETSDYNIRKWEKLVHPDDLVSSKKKIDLHLCGKEPFYNSELRMRHKDG
                     HYVWISDKGKTIIFDAEGKPETMYGINQDITARKEKEMEIEHLSYCDILTGIYNRRKF
                     EEVLREKDTEENLPISIVMADVDALKTTNDAFGHLQGDNLLKKVANVIKSTFNFTDLI
                     FRVGGDEFVILITNCDCEKTRKAAEEVKDALMKDSIGGLPCSVSFGIAAKKNPQESIF
                     EVYKDAEMLMYNQKLINSSEYHMNVIELIRENLFNEYPQEESHARMVTEFATKLAKEC
                     GLSETEVAAIGTLAEVHDIGKIAIGHELLKKNGKLEETEIIRIRQHPEIGYRILFSAV
                     GYNRIALDVLSHHERWDGHGYPKGLKEEAIPFRSRIIAICEAYSAMIQDWPYRKALGK
                     EVARREILEGAGKQFDPELARIFVEKVINQ"
     gene            complement(39630..40118)
                     /locus_tag="PLP02_01310"
     CDS             complement(39630..40118)
                     /locus_tag="PLP02_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42139.1"
                     /translation="MTAILLAILVVQEEYLVLLPNIQFTAVLLLVYAAILPWSLLCTL
                     VVSYVFIDNLIMGSFSLLYTIPMLFAWLFFVVIGKLIAKKSFFVILIFAFAFGFIYGW
                     FYFPTQMILQGVDILWPYFLADLEFEIVMAVCNYFTVLLLYRTLVALLEKLLSGWTAK
                     SG"
     gene            complement(40159..40578)
                     /locus_tag="PLP02_01315"
     CDS             complement(40159..40578)
                     /locus_tag="PLP02_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HPF42140.1"
                     /translation="MKRKALGIMLALILLPAFLACGNVTTSGTDTLTGTITFELYDAN
                     DGLISQKTIAYEESDTLLGLLQENYTVYCNDADGNPDDTCSYEYTFGHYIVGIGDLDA
                     YTDDTYYISFYINGQYSMTGIDDTPITDGYCYQFKLE"
     regulatory      complement(40595..40786)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(40853..41476)
                     /locus_tag="PLP02_01320"
     CDS             complement(40853..41476)
                     /locus_tag="PLP02_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HPF42141.1"
                     /translation="MIKIDLDTWPRKKHFMLFKDSDQPHFGITFEADVTPLVDFSKTS
                     KTSFFAVMLHAVMTSLNQIPEFRYRIRPDGVILHDIVSPSYTVLSDDDLFYFVTTDYC
                     DSLAQFVFKVQKDIEAAKIRKSLDDVEGKDDIVYISSLPWFSFTSLTHPSDTKHPDSF
                     PRISWDKYRMIDGKAMIHISVSAHHALCDGIHIYRFISNLKTYAEKI"
     gene            complement(41481..42311)
                     /locus_tag="PLP02_01325"
     CDS             complement(41481..42311)
                     /locus_tag="PLP02_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF42142.1"
                     /translation="MNIKKHIVLDNGISIPSLGFGTWQIKNGKEAYDAVFTALEAGYR
                     HIDTAAAYQNEESVGRAIRDFGLRREEIFLTTKLRAELKGYDVAMKELEMSLEKLETD
                     YVDLYLIHAPKPWGTKGDGSEYTEANIDSWKAFIKLYEEGKCRSIGVSNFQPYHITPL
                     TEATGFIPHVDQIFICPGNIPQETVDYAKEMGIRLEAYSPFATGRIFKSEELADMAKK
                     YGRTPGQVALRWSLQKGFIPLPKSTKSSRIRSNLDVFSFEISPEDMKVLDNLKFPKEG
                     "
     gene            42479..42856
                     /locus_tag="PLP02_01330"
     CDS             42479..42856
                     /locus_tag="PLP02_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPF42143.1"
                     /translation="MNKPIFSDLAPKAIGPYSQAIDCGDFLFVSGQLPVDPVTGNMEK
                     NDIKIQTKRSLDNVIAIANEAGYFKSDVVKCTVYLKNLNDFSQMNEVYQEFFSNHRPA
                     RVALEVARLPKDALVEIDAICHK"
     gene            complement(42905..43066)
                     /locus_tag="PLP02_01335"
     CDS             complement(42905..43066)
                     /locus_tag="PLP02_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPF42144.1"
                     /translation="MKKWVCEVCGYVYDPEFGDPEGGVAPGTAFEDIPEDWVCPDCGV
                     SKDNFSPLD"
     gene            43260..>44819
                     /locus_tag="PLP02_01340"
     CDS             43260..>44819
                     /locus_tag="PLP02_01340"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPF42145.1"
                     /translation="MPSNDILFSTIFCIVVSLLAFGLAIWLYVWVKKQESNNQKIDSI
                     GKLIKSGANTFLKKEYELLARFAGVVAILIFVFLPKPIWQSTGWADWSQNLTITLAYL
                     FGTMFSALAGKIGIFVATIANRKTAEAAQKGIKESFLTGFRGGAVMGMAVVGASLLGI
                     SIVFLIANQDATMLLGFSFGASSLALFAKAGGGIYTKTADISADLVGKVELGIPEDDP
                     RNPAVIADNVGDNVGDVAGMGADLFDSHVASIAAAMVIATTISTKMVSVVFVYAGLGL
                     LSSIIGVIFARMGKKGNPTTALNLSSYITMGIFTVLTALATLIFRFAWGIFGASVVGL
                     AVGAVVGLATDYFTDDRKSPVKKVAEASKSGPALTIISGVSYGFMSVLPAMVGIGLSA
                     FVAYILNPDYPLFGIAMSAVGMLSVVGMIISNDAYGPIVDNARGLAEMGGLGEEVIRR
                     TDSLDSAGNTVKAVTKGFAIGAAGLTVISLLGAFISEVNDAATERGLATITGFDIMNP
                     AVFFGLLVGAAV"
BASE COUNT        11172 a        10597 c         9416 g        13634 t
ORIGIN      
        1 ttcagcgttg tcgcccggta catcgcgccc gtgtccacat aggtgtagcc cagacgtttt
       61 gccaggagtt tcgccaccgt cgactttccg gcacccgccg gtccgtcgat tgccaaagca
      121 tgatatatca tacaatcgct cctcaactta atacattata ccacatataa ttgatatttc
      181 cagccccaat taaccgcaaa aatgttttca ttttccgttg ttcgagagat tccaaagttt
      241 tcttatctcg tgcaatttta aaagacgata gcctcccagg gggatgcctt ccgtcgtaac
      301 acatcccatc cgatatcgat gtagtttcat tactggatgt ccgacggctt caagcattct
      361 tttcacttga tggtatttgc cttccgtcac gattattttc agatatgtgt tttctttggc
      421 gtcatcgtat tttacatcaa atactttcgc aggttggcta acgaaatcgc ccaaatcgac
      481 gcctctagct aatttcgccg aagtttcctt gcgaaccaat cctttcactt tcagatgata
      541 ttctttttcg actttgtatc gcggatgaat aagttggttc atgaagtcac cgtcgttcgt
      601 cagaaggatg atcccagaag catcgtaatc aaggcgtccc accggaaaaa ccctttcttt
      661 tccctgtacg agatcaatga ctgccggcct tccgtgttcg tcggaggtag ttgaaatgta
      721 gccggtcggc ttattcaggg cgtaatatac tttcttttcc cgtttcagtt cctttccgcc
      781 aacaaaaatc cggtcgttat ctgcgaccat aactcccatt tgggtcgtta tgacgccgtt
      841 aaccgaaact tttccttccg caatcatttc ctcggctttc cttctggaag ccactccggc
      901 ggcctgcaac actttttgta aacgttccat atccggcata cctcctttct tttcccaaaa
      961 aatgaggata gtcgcctatc ctcatcaaag atatcagctt tcttccgctt cggtttcctt
     1021 tggcagaagt tcgatcgtca acaagacgga tttgatcctt cttttcgaaa tcgcaagaat
     1081 ctcgaaagtc aggagttcgt gatcttcgga aaccagttcg gacaaatcgt cgtaaacctg
     1141 gttgaccgaa agttcatatt cgattctctc cccgacctcg ggaatgtcct ggaatttttc
     1201 aaacaaccat cccccgatcg aagtcgcgtc ggagacgggc ggttcgccta gttccaaatc
     1261 ttcgaaaagg tctttgagat catagtcggc gttgacgaca tattcgttgg gagctttctt
     1321 tacgattgct tccttgacat cgtcgtgttc gtcgtaaatc tccccgacaa gttcttccaa
     1381 ggcgtcttcc atggtcacga gaccgtcgac gccgccgtat tcatcaacga caatcgtcaa
     1441 atgagagttc tcggcctgca tgagttctat aagggcgtcg accttggtcg tcgatggtac
     1501 gaagatcggt ttcctcaaca gttttttgat attgatgcta tggcccttga tcagcttggt
     1561 ataaaaatcc ctctcggaaa gaatgccgat aatgttgtcg gaattgccgt catagacagg
     1621 tacccgggag aatttgcttt tgaagaaagt atcgcggatt tccgacaccg gggtcgaaac
     1681 atccagagcg atcatgtcca ctcgcggcgt catgatctct tcaaccgtta tttcggacag
     1741 atcgagaaca ttctgaagca tgtcggcttc atcttcatcg atcgagcctt cttcttccat
     1801 cgtgtcgatg atggtctcga gttccgattc ggtcaccgtc agtttatttt cggtttcgat
     1861 ctgctggatc acgcgatggt tcagaagcct gaaaggatat gtgagcggtg tcatgatctt
     1921 gatcaaaaaa tagacaagcc cgctgaaaag caggcaaatt ttttccggat gcgtcttagc
     1981 gacgctcttc ggaagaattt ctccgaatat caggacgata atggtgatcg ccacagtatt
     2041 gattatggtg acaaccgtat cggcatttgt caaatccaag aagagtttgg caaagaaaag
     2101 tgcactgacc gaagtcaggg caacattgga tagattgttc ccgacaagaa gcgtagtgag
     2161 cgtcttgtcg aagttttcaa ctatccagag cgcttttttc gagcctctgg cattatcttc
     2221 gaccaatgtt tttattctta ctttaccgac cgccgagtat gccatttccg ccatcgagaa
     2281 gaaagcggag aaaaagagca ttgccagcat tatagccagc agacccggtc ggaaagtggc
     2341 ttccaatgtt acaagccaca tgtgacttgg ttccaagaaa tcatcactcc ctgttgatta
     2401 aaaaactatt ttgctctggt atcgtatttt ccagtggaag tcgataccac gattttttca
     2461 cccggctcga tgaataaggg aaccagaagc cgtagcccgg tgttggtaag agcttctttg
     2521 gtagcgttgg aggcagtgtt tcctttagct ccacccgctg tttcaactac ttcgagaatg
     2581 accttgtcag gcagttcgag gcctagaatc tcgtgctcga aactgataat gttgatcgac
     2641 attccctcgg tgagataata caattcgttc tcgatctgcg atttatgcaa ttcaatctgt
     2701 tcgaaagttt cgttattcat aaaaacatgc gtgtcgccat tggcatagat gtactgcatc
     2761 tgtgatttgt cgattagcgc ctggttgaca ttaaccccgg cattgaacgt tttgtcgaga
     2821 atggttccgg aacgtaggtt cctgagtttc gtcctgacga atgcgcctcc cttgccgggt
     2881 ttcacatgct gaaattcgat cacttggtag agatttccct cgtattcgat cgttagtcct
     2941 gttttgaaat cgttggtgct taccatttat aaatcctccc gcggtaataa tattacggat
     3001 ataccttaaa tattataata taaaatactt tggttgacaa ctcatttcaa ctctactata
     3061 atgatgattg tatcttcgcg aggtgagaat catgcagttt ttatcagtta cggactggat
     3121 ttggcctatt tccatcggac tgttggtcgg catcctgata tccgccagaa aacgtcacaa
     3181 ctacaacgat atcgtatatc tcgaggcaga tgaattccga cagaacatgc ggaagggtca
     3241 attgatcgat atccgcaacg aggaagcttt caagaaagaa cgcatcaacg gcagccgcaa
     3301 tttccccggc cgatcggttt tttccaacct caacaggctt cgcaaggatc aaccagtgtt
     3361 tctgtattcc gacaccgatt cgttcaaagt cagacaaacc gcaaaaaaat tcattcgcaa
     3421 aggctttcaa ccggtctacg tattaaaggg cggccttacc gtctggagtt tccctttgaa
     3481 aaacgattga acccgataat aaaaccaaag gcgtggaatc agatttcctc gccttttttt
     3541 tctatataat tgacaatctc atcatatagg gtgatcctgt tgtcattcgt cacatcgaac
     3601 caagtcacat ccatctggtt cttaaaccag gtcaattgtc ttttggcgta tcttctagaa
     3661 ttccttttaa tcaagtcgaa tgcttcttca ttacggattt cattacggaa aaacataaag
     3721 agttccttgt agccgatcgc actgatcgat tgtccagtta tgcctgcttc aaaaagggct
     3781 tttgcttctt caaccaaacc gttttcgatc attctgtcca ctcgttcgtc tatttgtttg
     3841 tagagttcct ttctgtccat tctgagcccg ataaggagaa tgttatcgta aaagggaatg
     3901 gaacctttct tacgaaattc gttcgatccg gtgccgacga tttccaggct tctcagaatc
     3961 cgtctccggt tgttgggatg gatcgttttg gcgagttgcg gatcctgttt ttctaaaatc
     4021 ccatacagtt ccgaattatc gtaatccgaa tattcggcag tcaactcatc gtttcttttt
     4081 tccccaggaa attcatactc gtaaagagcg gccttgatgt aaaaacctga tccgccgaca
     4141 acaattggca tcctaccttc tttactgacc aattcgatct tctcccgtac cattttctga
     4201 taatccgaag cggaaaaaga ttcattgggt tctaatatgt caatcaaatg atgccttact
     4261 ccttgcattt ccgttttttt gatcttagcg gtcccgatat ccatgcgttt atatagctgg
     4321 aatgcatcag cagaaataac ttcaccgtca tactttttcg ccaatttgac cgccagatcg
     4381 ctttttccgg tagccgtcgg tccgacgatc gccaaaatcg gtttcacgag gccacccggt
     4441 aaaaccattt ctcgatgtca tactcaccga tcaaaacaat aatcggtctt ccgtgcggac
     4501 atgtatatgg tctattacat ttagaaagat ctccgacgag tctttcggct tcttccctgg
     4561 ataaacggtg gttggctttc aacgaccgtt tgcaggcaag aagttttgct gtttcgtcga
     4621 ccagtgtctt tttatcggtt tttccttcct cgatcaaaga tgaaacgaca gtttccgcaa
     4681 aatcggcttc gtgtcctttc ggataccagg aaggaactgt ttccaatagg attgtcgaaa
     4741 gatctttttt tctgatcaca agaccgtttt ccttcgcttt atcgattgta tctttcccga
     4801 tcatttccgt cagggtgttc gaaagcgata gttccatcgg caacaaaagc tggtaggtct
     4861 ctttatttgg agccaacatc aagtttagat acttctcgta ccttatccgt tcagccgcag
     4921 cgtgctggtc tactagatat agtcccgctt cgttctggaa caagagatag gttcccccat
     4981 attgcccgac atattcgaaa tcgggaaatt cattacttgc cttatcctct tttggaatct
     5041 cttcctcttc tttgacaaaa agaccttctt caggttcttt gtttggataa gatgttccaa
     5101 atcccatttt tatctgtgta tcgcttttat cagaagccgt ctggatgact tttgttttcc
     5161 cgatggcttc tttcagggtt tcgctaatca gtttgcgaag tgtttccagt tcggaaaact
     5221 tgacctccgc tttggtcgga tgaatgttca catcgatcaa ttgtggatcg actgcgatat
     5281 tcaaaagaat tatcggatat ttgtctttgg gcagatatcc gatgaaacat tcgacgattt
     5341 ctcttgtcaa gataaaatcc ctgatcggtc ttccattcac gatcaggatc accgattgtc
     5401 ttcccgagcg gttgtgaatt ggattggcaa aataaccttc gatggaataa tcgcggtttt
     5461 ttccggaaaa acgtttaacc gacttcgcta cttccagacc gtagacaaca gcaaatatat
     5521 cggtttccga attgtttccc gatgtctgca aaagcattct tccgttgttt tccagccgga
     5581 aagcgacatc cggatgagca aaggccattt tgtcgacaaa gtcgacgatc agtctcagtt
     5641 cgaccgtttc cgtcttcagg tatttgaaac gggccggcgt attgaaaaaa agcctggtga
     5701 cggtaatcgt tgttccgtcc ctggtggctg tttcttcatt aacaagcagt ctcccgtctt
     5761 cataaacgat tctggttccc acttttttgt cggatgtctc catctcgaca cgggatacag
     5821 atgcgatcga aggtaaagcc tcgccgcgga aaccgagcga ggttatgtgg aataaatcgt
     5881 attcgttttt tatcttggaa gtcgcatgcc gggaaaatgc gagcagggaa tcctccggat
     5941 ccattccgga tccgttgtcc gaaacctgga ccatggcgaa gccggagtct ttaagcgaaa
     6001 tctggattct ggtagctcct gcgtccaagg aattctcgat cagttccttg acaacgttga
     6061 cgattcgttc gacgacttcg ccggcggcga tcatgttttg gagtctagcg tccagttgga
     6121 tgatgtgtcc catttcgtca tcccctttca gttgatcttt ttgatttcct cgattatcga
     6181 tgccaggata ttcatggctt tcaacggggt aagttcgttg atttcgattt cctttatttg
     6241 atcgatgatc ggacggtatt cgcttttttc tctttcttct tccagagaag cggtttcaaa
     6301 attgaaaaga tcgaccgtct gaggtttgat gacattatag ccgtgattct tttcgagatc
     6361 ttccagaatc gtctgggctc tgcgtaccag ctgggttggc agtttagcca gttttcctac
     6421 atggattcca tatgagcggt ctgtcggtcc gtcggcgact ttgtgcaaga aaacgatgtt
     6481 tcccttgtcc tcttttgcca tcacatggat gttatgaaga cttttcaggg tatcctccag
     6541 gtaagtcagt tcatggtaat gggtggagaa aaggactttg caagcgatct tgtgatgggt
     6601 gtattcgatg atcgcctgtg ccagagccat gccgtcgtag gtggcggttc ccctgccgat
     6661 ctcatcaaac aaaatcaaac tgttttcagt ggcgttttgc agggcatagt tcacttccat
     6721 catctcgacc atgaaagtcg attttccggt cgataggtcg tcgctcgcgc cgattctcgt
     6781 gaaaatgcgg tcgaataccg gcagtaaagc tgattttgca ggcacaaaac agccgatctg
     6841 ggccatgatg acgatcagag ccagctgacg catataggtt gattttccgg acatgttcgg
     6901 tccggttatg agcaaaatcg aagtatcgct gtccatattc aaatcattgg atacgaatga
     6961 tcctctggcg actgtccgtt cgacaaccgg atgccttcct tcgacgatca tgatcttttt
     7021 ttcattagag agaactggtc gtacatagcc gttttcgatc gatattatgg cgaatgccgt
     7081 cagcatgtcc acttccgcaa gcgatttcgc cagtttttgc agattatcgg tcattccttt
     7141 cgcctgttcc ctgacaaaac agaaaagttc atactccatc tggattgatt tgtccatcga
     7201 accgaggatc atcgattcct tttccttaag ttcgggggtt atatatcttt cacagttgac
     7261 aagcgtctgt ttccggtcgt aaccgaattc cggtttgatc tgatcgatct gtcccttcgt
     7321 cacttcgata tagtaaccga aaacccgatt gtatccgatt ctcagttttt tgattccggt
     7381 ccgttcgcgt tccttttctt cgaagtcacg gagccattct ttcccgtggg tggcccggta
     7441 ttttatctcg tccagttcgg aagaataacc agctttgatg atgccgcctt ccttgaccga
     7501 aaatggcgga tcttcgacga tcgcttgtcc gatcagatct tctagttgtg tgaagtcttc
     7561 cattcctttc aaaaggcggt tggcgaggtc gtcttccaga gacagaagtc tcgatttgat
     7621 cccgggaacg cttttaagcg atcttgccag gttgacgagg tccttgccgt tggcgttgcc
     7681 gaaagaaagc cttccgacaa tcctttccaa atcatatact tccttgagaa gatttctgat
     7741 ttcttccctg ataatgaaat tgtcgctcat cgcttcgacg agccgatagc gttcctcgat
     7801 tttttctttg tcgaccaaag gacgcatgat gttgcttttg agcatcctcg atcccattgc
     7861 gcactggcat ttgtcaagta gccagaaaag cgttcctttg cggcttaggg agcgatatgt
     7921 ctccgtcaat tcgaggttgc gtctcgaatt gttgtcgata tgaagatatt tcgaggactc
     7981 gaacacctgc actttctgca tgtgcatcag ttcgcgcctt tgcgagctgt aaagataatt
     8041 catgagtctg gcgaatgtct gtagcagttc cttgtcatag atatcctgaa caagactctt
     8101 gcaataggcg ggaagttcaa gattgtccga tatggatacg attaacgcat aactgttcag
     8161 atattctctg atcctgcttt ggttgaaatc actgcccagc accaattccc tgacattcag
     8221 attcaagatt tcattcatca ggatgtcgat gtcgtttggc aatgtgatga tgtgattttc
     8281 tccggtcgtt agatcggaat aagccaaaag gaactgtgtt ttcgtttccg atatcgctgc
     8341 gataaaattg ttcgattttt cctcgactgc gccttcctcg acgatcgtcc cgggagtgat
     8401 cagtttgatg acatcccttt tgacaagtcc tttcgcaagt tgcggatctt cgatttgttc
     8461 gacaatagcc accttgaatc ccttgtcgat cagtttttcg atatagaaat tggccgcatg
     8521 atggggaacc ccgcacatcg gtactttgtc ctccatgccg gcatcccgtc cggtaaggac
     8581 gatttccagt tctttcgatg cgacgatcgc atcggtgaaa aacatctcat aaaaatctcc
     8641 cagtcgaaaa aagactataa agtcgctata gtcttttttg atttccagat attgttgcat
     8701 catcggagta tagtttgacc ggtcaagcga attatgatcc attgatttag attctccctg
     8761 aaaacccgct gttattcaaa aagtccgaac ggccaatggc cggggttcaa cgtaaagtga
     8821 tatacataag tgtaggcacc gataatgatg atggcggcga agagtgtgat gaggaagatg
     8881 gtccaaaacc gttttttccg ctttttctga tggtattttt tgatgatgag attgatcttt
     8941 tcgtcaagca gatccttttc ctcgtcggaa aattcgatga tctcgctttg tggtttttca
     9001 gcttcggtag ctgttttctc caaaaccggg gccacgatcg gctcttcttt cttttcagga
     9061 ataatttccg gttccggtga gattaccggc tttttcgtta cgatttcttc agtttttttg
     9121 atctcttccg gcatcttttg ttcttcttca ggcaactcga ttgtttcctc ttccttcacc
     9181 ggctcttctt ccggcttaag aatcgtttct tcttttgcaa ccgaaatcgt ttccgccggt
     9241 tcttcgtcaa cttcgatact tcgcatgatt tctttttctg ccggttcggc ttcgattacg
     9301 ggaatatcct gatcgttggc ctggaactcg atcgcgtcga tcttgaccga atcaaattct
     9361 gactcgagag gaatattgta atcatggaca tcctttgcga tttcctgatg ataaagattc
     9421 agggcataga tgatgtattc caccatgtcg cttttcttga gatcgatcga aatctggaag
     9481 cctttttcct tcgcatagat ttccaattcg agaaccgatt tgtcctgaag cagcgtggct
     9541 tcctgttcgg ttagccgaaa acgggccgca aggatctcga tcaggctgga cttgttgatg
     9601 cggcgtggta ctttgacacc gtacttttca ccgagaccgc gaagatcgcc taaggtgcag
     9661 gaatgcacga agacatcctt gaggattttg agactcacac cgtcgagata gccgttcggt
     9721 tcgtagatta cccgctccat catgccgatg aaatcgccgt aattgatttt ttcgagatcg
     9781 acggcgtatt tgtatttcag cttttccaga tcatcgaaaa aagcgttctt caatccgtaa
     9841 agctcacggt tgagaaacag aatgtcgaag agcaattgtt tgtacttggc ggcatcaaaa
     9901 tcaagattgt agtcgtaaat gagtttctcg agcaaataaa ccgaatagtc gttaaacccg
     9961 cgcagccggt agcgaagctc gtctgtgtag gactcgtaaa gtttggtttg aaagactttt
    10021 gggtagatcg tttctcttaa aacgaaacga tgaatgtaag taggaatcat aatcttgtgc
    10081 tgcaacaaca attcgagcaa atcctctgtg ggtattacgg acaatgtttt ggcaaatgca
    10141 aaatagtcct cgttcgcttt gaatctactc atggtctcac ctttttcatt acaatatact
    10201 ttgatattac tatattttgt tttcttattc aagggaaaaa acagaaaatt tggcgatttc
    10261 cttgtttttt ttccatatcg cttttcgcta ttctttttca gagccccaat gaggtctcgc
    10321 ttatttgttt gcttctttgt ccgtcacttc gccatagagc gaccaagtgt tggcttttgt
    10381 gatattaact gacactgttt ttcctaccat tccggggttt cccggaaagt ttacgagttt
    10441 gttgttggga ttataaccac aatatttcgt cgcatccgtt ttggactttc cttcgaccaa
    10501 tacttccgtg gtcgtgtcga gaaatctcct ggtaccggac gcatagccgc gattgaccag
    10561 ttcgttcagt cttttcaagc ggtcgcgttt tgtttccgcc aacacatcat cgggcataga
    10621 agccgccgga gttccggctc gtttcgaata aatgaaagta taggctcctt cgaagccggc
    10681 ttttttgacg agatcaagcg tttcctggaa atcggcctcg gtttcacccg gaaatccgac
    10741 gatgatgtct gtagtcaggg agatgcccgg aatctttgtt ttgattttgt ttacaagttc
    10801 cagatattgc tctttcgtgt atttgcgatt cattttggaa agaatggcat tcgatccgga
    10861 ttgcaccggc aaatggatga acggcatcag gtttccgcct ttcgccagaa tatctatgag
    10921 ctcgtcggtg aaatccttgg gatggcttgt catgaaacgg atccgttcga tcggtaattt
    10981 gtttagttta tcgagcaatg cggcaaatgt gatttccgga tcgccgtaat ccagtccgta
    11041 tgaattgaca ttttgcccta gcaatgtgac ttcccgataa cccttttttg ccagttggga
    11101 aacttcttcg acaatatctt cgatctggcg tgatcgttct tttccccttg tgtaaggaac
    11161 gatgcaatac gtgcagaatt cgtcgcatcc gtaagtgatg tcaacccaag ctttgaacgg
    11221 atggttgcgg acggaaggaa tcttctctac tatgtctcct tcttcggata gaacttcaac
    11281 aaccattttt ccgctttcta tccgttcttt gatcatttcc ggtagtcttt cgatgttgtg
    11341 agttccaaac aggatatcga cgcgattctt ggttttcaat atcttctcga ccgttgtctc
    11401 ttcttgcggc atacagccgc caagagcgac gatgagatcc ggattggctg ctttgtattt
    11461 catgatgcgg ccgatttcgc catacacccg cttctcggcg ttttccctga ttgcgcaagt
    11521 attgagaatg actatgtccg ccctttcgat acaatcggcg gcttcatatc cgaccatgga
    11581 caaaagaccg gccattcttt cggaatctgc ttcattggcc tggcatccgt atgtctcgat
    11641 aaaatacttt ttgtcctttc cgatgcaatc gatgccggaa gaaggcttat attgccggat
    11701 ttccaccggt ccctttgtac gttttctcgc tgatttcaat tctggtttga tcattcgact
    11761 atctcttttc cggtaaatca ttccgcatag ttcacccgtt cgatccgttt ctgttcgaga
    11821 tcgagaagta cggcgctaaa ctgaagtggt ccgttttttt ccggtcttgt cctttcggga
    11881 attccggtga taaatttacg taggccctga ccgatgtcgc caccgattat cgaatatttg
    11941 actcccgtca ttccgatatc ggtaatgtag agcattcctt tcggaaacac gcattcgtcg
    12001 gcggttgtca catgggtatg cgttcccacc aaagcgctta cccgtccatc aagataattg
    12061 gcgagcgcat acttttcgct ggtggcttcc gcatggaaat caatgataat ataatccgaa
    12121 tcgcactttt gaataacttc atcggctacg atgaaaggac tgtcgatcgg atcgttcatg
    12181 aaaacccggc caagcaggtt gaccacggtg attgtcttgt cgttcacccg gcaggttaca
    12241 aatcccttcc cagcggcgcc tggtccgtaa ttggctggcc tcaccacata gtctaggtta
    12301 agaacctcac cagaatcctt gcgggaatag gcatggtttc cgagtgtgaa cacattgact
    12361 cctcttttca tgtagtcttt gtatatttct tccgatagtc cgttgccgtt ggcgatgttc
    12421 tcaccgttaa cgacaatgaa attcggccgg tagagttgct tggcagtcga gaaaaagcga
    12481 tcaaatgcct cgcgaccttt ttccatatag acgtctccga taaacagtac gcgcatgttt
    12541 ccaccctctt cttaacaaga tagccttcct ttcgggaagg ctatcaaatc tcattttgcg
    12601 atgtcttgag cccttgtctc ccggatgacg gttatcttga ttgttcccgg atagctcagt
    12661 ttctcttcaa tttgctgttt tatttctctg gcgacgacaa atgtcttggc atcgtcgatt
    12721 tcgttcggtt tgacgattac gcggatctca cggcccgcct gaatggcata tgcctggtcc
    12781 acaccttcca cagaagtgga tatttcttcc aactgggtga gacgattgat gtagttttca
    12841 agcgattcgc tcctggctcc cggtctggct gcggaaaggg cgtcggcggc ggctactagc
    12901 acgccgataa cggtttcagc cggtttgtct ccgtgatggc tggcgattgc gtcgatgatc
    12961 ggttttgtct cgtgatattt gttcgcaagc atcacgccga tgtcgacatg cgatccttcg
    13021 atctcgtgat caacggcttt cccgatgtca tgaagcaatc cggcccgttt ggctatcact
    13081 tcgttctcgc cgagttcaac ggccatttta ccggccaaat aagcggtctc gatcgaatgc
    13141 tgcagggcgt tttgtccata agaagtgcga aaatgcagtc ttcctatcag ttttacgaga
    13201 tccggatgaa ctttgccgat accggtttcg aagacggcct tgtcgcccat gtcgcgaatc
    13261 aattgatcta cttccactct ggttttctcg acaatttctt cgattctggc cggatggata
    13321 cgtccatctg aaatcaatgt ttcaatcgag cgtttagcga tttcccttct taccgggtcg
    13381 aatcctgaca gaactacggc ttccggggtg tcgtcgatga tcagatcgac tccggtcatg
    13441 gcttcgatcg ttctgatgtt tcttccttca cggccgatta tccggccttt catttcatcg
    13501 tttggcagat tgacaacgga tacggtcgat tccgcggtca catcctgtgc gtatttttgg
    13561 attgcctgcg ataaaatttc cttggcgttt ttctgagcgt cgagtcttgc tttttcttcc
    13621 tcttctctca aataacgtcc gatttcctga gccatgtcac tttcgacacg ggccatgatg
    13681 atttcttttg cttgttgttc cgaaagattg gcaatatcca ggagtttctt gttttcttcc
    13741 tggatgattt cgtccagttt gctgttcttt tcctcaagag cgactttttt ctcgtcaagt
    13801 atttcctctt tacgatccaa gttgagttct cttttgtcca aatttgccga acgacgatcc
    13861 attgtttctt cgcgttgatt aactttgttt tccagttcgg aaatcaacgc tttcttctct
    13921 ttgattaatt tatcgttctc ggaattcaga ttgtagattt cttgtttagc ttctaagagt
    13981 ttttcttttt tgattttttc cgcttgagaa tttgcttcgt caatgatgtt ttgcgcttta
    14041 tttttcgctg tttggaatcc cttttcgacg accgataccc gagtaaggaa tccgatgata
    14101 ccaccgacaa ttaagcctag caaactggaa ataatttcag gccatggcat gattattacc
    14161 tccgagttta gaattgtcat atatgatagc cgggctcgta ttgtgagtct ggcacctata
    14221 tcttgatact attataatat aaaaaaatat gatagtcaaa aattttttcc gggaattcca
    14281 atgtttccaa aaaaaaagga aagttgttcg gactttccta tttaagattc agttcttttt
    14341 ttactaactg acggatttct tcgtaaacat ccggattttc ttccaaatat ttcttgacgt
    14401 tttctcttcc ctgtccgatc ttttgatcct tataggaata ccaagcgccg cttttctgaa
    14461 tgatgtcttt atcggcggcg agatcgacca gttcgccggt cttggagatt ccctttccga
    14521 acatgatgtc gacgatgcaa gttttgaaag gcgcagcgac cttgttcttg acaactttga
    14581 ctttggcttg gtttccgatg aattcgttgc cttccttgat ctgttcgcct cttctgattt
    14641 ccaagcgtac cgatgcataa aatttaaggg ctcttcctcc cggtgtcgtt tccggattgc
    14701 caaacattac gccgactttt tccctgatct ggttgataaa gatcgcaatg cattgcgatt
    14761 tagataaaac gcctgacagt ttacgcatgg cttggctcat cagtcttgcc tgcaatccga
    14821 catgtgaatc gcccatctcg cccctgattt ccgcttccgg gaccaaggcg gcgacagaat
    14881 cgacgaccat tatgtcgaca gctccgcttc tgactagtgc ttcggcgatt tccaatccct
    14941 gttcgccggt atccggctgg gaaagaatca aagaatcgat atcgactccg aggtttttcg
    15001 cataaacggc atcaagcgcg tgttcggcgt cgataaaggc ggcatagccg ccgtttttct
    15061 gcacttctgc gatcgcatga agtgcaaagg tcgtttttcc cgaagattct ggtccgtaga
    15121 tctcgacgat ccttccgcgc ggatatccac cgactcccaa agcgctgtca agcgccaaag
    15181 aaccagtaga aaccgcttct atgctcatgt cgtcctgtct ttctcccaag atcatcacgg
    15241 cacccttgcc atattctttt tcgatttcct taagtgcgac ttcaagaatc ttctttcttt
    15301 tttcgtcagc catattagcc tcccgtttcc aagattgcga tattcttcat taataatacg
    15361 ccggacaatc cggctttgct ttaaaatgtc tcgaaaataa gtttgcggtt tttgatgaaa
    15421 taatccactc cggaaatcac cgtcattaga atggcgatgc ccatgataac ccatccggtg
    15481 atgttagccc ataaaagatc gtatggcatc atgaacagat accaggtgat tgcggccatg
    15541 gtcgatgcgg ttttgaattt tcccagttgc gatgcggcga ttatttttcc gtttccgacg
    15601 gctacgagtc ttatcgacgt gactatcagt tcacgggcaa gaacgatgac aggtacccag
    15661 aagggcaacc acatattcgg aacaattcga taattgtccg ccagaacgat cagggcggac
    15721 aagaccaaaa gcttatcggc aagcggatcc ataaacttac cgaatgtcgt gaccatattg
    15781 cgttttctgg cgatttttcc gtcgaaataa tctgtgattg aagccaggac aaacagtatt
    15841 cccataatcc agtatgtcat gtttccaagt gtgcctttaa gcagataaac gacaatcaag
    15901 agcggcacca ttatcatcct tacaatcgtc aagcggttcg gtagattcat tttgaacacc
    15961 tcatatataa ttatatcata tatggcctgg aatacgccac gaaaaacgat tttttcctaa
    16021 gaaaaaagcg gtcgttaaac cgcttatgtc cggtattatt cctcttcggg gatattgacg
    16081 ttgtgataga tctcctgaac atcgtcgaga tcatttgtca tattaagaaa acgttggaat
    16141 ttacctaggt cttctggggt aagagtgata ctctccaagg gaatgtaagt gaccgtgtct
    16201 tccagataat tcagctcttt tttgctggca tccaaagcgt ccttgatcgc gtctaaatcc
    16261 gcgggagcgc cggtgatcat gacaattccg tcttcttcgg cgatatcggc gatatcgatt
    16321 cccgcttcca gtatcgcttc caaagcttct tcttcggata tgccttccac actgattagg
    16381 gacgaatagg tgtaaagata agtaacgctt ccagaaactc cgagtttacc accggtcttg
    16441 gtgaagcaac ttctaacttc accgaatgtc cggttgacat tgtcagtcag acactcgacg
    16501 atgaccgtcg ctcccccggg accgaagccc tcgtatctgg cgggagtata atcttctccg
    16561 gctccgcctt tggccttgtc gatattcctt ttgatgacat ctccgggaac ctggttctgt
    16621 ttcgctcttt cgattgttct tttcagtacg aggttgccgt cgggatcggt tccgccggtc
    16681 ttggcggcca tgtatatttc tttaccgaat ttcgagtata atttgctttt catcatgctt
    16741 gttttttcca ttgctgcttt tcttacttca tgggctcttc ccataatttc accaccattg
    16801 ttttattttt tgcttttgca agccatctcc gtatgatcgg gatacaatac tatcctaccc
    16861 tcgcggtaaa gatgtccgat cgcacgtttg aatgccgatt tgctcattcc gaaagccgat
    16921 tctatctctc ccggaagact tttatcgctg tatctcatat atccgtcgtg attttccaaa
    16981 tatgcaagga tcgtcctggc atcaggatcg atcatcgatt cttttctttc gatcagagat
    17041 gcgacatatt cgttgtcgct gttttttttc acgattctcg gagcgatttg ctctccgatc
    17101 cggaaattgt ccctgagatt gtttttatgg atgaatattt cctgcccttc gctagcgaaa
    17161 gcgacaaggc cttccggaag gatgaacatc acccaggcat ctataacctt ctcaagatcc
    17221 agttcccgat gttctttaaa aaatccggtt atctgttttc ttcccggaat atgggcgaat
    17281 aactgccctt ttttttccgt catgacagcg aatacgctat caccggcctg tggccaaaga
    17341 tcaaggtcaa atggcaagtc gtccttgcta agtaacagat ccttgacctg gttgttgtct
    17401 agaaatacgc catattcgta attgacgcct ttcaccttga gaaatgaagc cgttcccagt
    17461 tccacaaacg gacaaaacgc cgaagcgcat attctgccat aactgtccgg atagatatag
    17521 gcatgaacga tgtccccggg cttcaattcg cttccggttt cttttttgtg gagaaagatt
    17581 tcttcttttt cgtttgtgag aatataggct atgtcggtta tccggctgac cttcagatca
    17641 gcagtttttc ctatttgcaa agtcatataa ggcccaaagg caccatggca agcaaaaaag
    17701 cagaacgccc ccggttgatc gacaacgaac gtctatcgtt accggaaccc ttctgttgga
    17761 aattgcttat gtcaaatgtc ttttccttta acatggtatt atctccgaac tgcttttctt
    17821 tcgttaacat tataccaaat cagaatgtta atgtccacct gattatatca ttaaaagaac
    17881 agggccaaat ccctgttcct cttcatgtta ttatttgtcc acggaataat ctctgatcag
    17941 gcttttatag aagttcaccg cccgatacag gctttcaatt tccagcgttt catcttttcc
    18001 gtgcatcttt cccaaatcct cgtcactgat gatgacgggg gaaaatctca gattgtcatc
    18061 ggtaatcaaa gcatagtggc gacagtccgt cgcaccggtg atgatatagg gagataccag
    18121 agcttctggg aaattctttc tgatttgtct ttctatcgac tggtatgcct tgctcctggt
    18181 atcgactatc ttggaagcgt cctcgctttc cagtatgatc gtctcgatat cgtattttgc
    18241 cgcaatcttc gacaataccc tataggtatc atcgacattc tggatcggat ggattcttat
    18301 gttggcgatc acattggctt ccgaaggaat cacattataa gcatcgcttc cggatgacat
    18361 cgtgaaagcg atagtagtcg acagaagcgc ccgaccaaaa ggactgattt taggaagcag
    18421 cccggtcatc aatcctttga aaagccacaa attgccgaac aagaaccggt aaggaaatga
    18481 cattcccgga gcggcattag cgaaaatcgc cctgactgag ggaatcatct tcgtttttag
    18541 aggaaaatgt ctttcgacat cgttcacaaa catagccaat ctggcaatcg gagtgttccg
    18601 gggcggagtg gaagaatgtc cgccttccga ttttgcttta aacatcatgt tcacatatcc
    18661 tttttcgaca actccgacaa aagccatggg aatttttatg gaaggaagcg agtctctgac
    18721 aaccgcgccg ccttcatcca gaacgaaagc cggcttcaca ccaatctgtt tcagatattc
    18781 ggctgtgtct ttcgcaccgt gtccggatac ttcctcgtcg cttgcgaaag ccagatagac
    18841 atccccttca ggaacgaaac cttctttgat tagttcgttt attgcctgca ttatcgcaaa
    18901 aacggtcact tttgtatcga aagatcccct gccgtagatt tttccatttt cgatcgttcc
    18961 ctcgaaagga ggataactcc agacattttc gtcagctggc accacatcct ggtgcgccat
    19021 cagaacgatc ggatcttttt ttcccgttcc tttccataag aaaagcagag aattattggg
    19081 aaaaaccttc ttttttgcca aagcgtgcag ttcgggaaac attttttcca tttccgattg
    19141 cataccgata aactcatttt ccgttccgga tatgcttgcg atcgttttat atcggagcat
    19201 ccggctcaag ttttcggcat attcttgcat cttgggagta aattcggaaa aatccggttt
    19261 ggcaaccggc tcaactttga ttaagaaagt tctgataatg gctaccgcca aaaggatagt
    19321 cactatcgaa catagtccga taacaagata ccagtaccaa tccataagta cgcctccgaa
    19381 tacgatcagt tatttttctt ctttttctct ccgagataac gctcttcctc actgaagacg
    19441 catccgcagt atttttgcat ataaaggggc agttccttgg caatcttcct tccttcgtaa
    19501 aagaaaggac ggaaatcttc atagtagaac gggattccct cttctttgcc gatcctatca
    19561 gcgatttcta ccaaaagggc atgcttctga taaggactta ttaacaatgt tgacgaaaaa
    19621 gcgtcaaatc ctcttttctt tgcttcgatg gccgtctttc tgattctctc ttcataacaa
    19681 aacatacagc ggttgttaag atccttggcg acatgctcga cgaacatcct gagtccgtat
    19741 acatcgtttt cgaccagatc gaggccgatg tcggtggcat agccttccaa agcttctttt
    19801 cttgctttat attccatata gggatgaata ttggggttgt accaatatcc ggtcacatca
    19861 tggttttgct ccctgagttt tttgatcacc atcacgctgc acggagcgca acaaatatgc
    19921 aaaaaaatac gcattactga atcacttctt tcatagcatt ttcaaacggt cgagagccgg
    19981 cttgtaatta gatctagccg agagttccag gtattgtctt gcccgagcca gatcggttct
    20041 tattccttct gcgccgttta agtaaagaat tccaagttca tacagggcag ccggttcctt
    20101 ggcgtcgttt gcgctttttt ccagccactt catggccggg acaatttccg tagcccgtaa
    20161 tagataatac atttccagca tcgcgattcc ggaagctcct gatgcggcaa gttcgaacca
    20221 gtaacggctt cttcttaagg atttctgagt tcccaaacct tctttgtaca taatggctat
    20281 ctgggaacaa gccatagcca cattgctttc aagatgttgc gggtatttga catattcgcg
    20341 aaaaagatct aaagctttgg catacatctt ctgatctttt ttctgtttat tctttgcatt
    20401 gtcgtacaga catgtcgcat acttgaaagc cgaagcgctt actccggcat ccaaagcttt
    20461 ctgataatgc ttgatggctt ttgaaatgtt tatgggcgtt ccgactccct tttcgtaata
    20521 gatacctagc cagtagtgag ccttgggagt tccctttttc aaagcatttt ccatgtactt
    20581 gaaggcaagt tcctcgctgg aatttgttat atctttccgg taatgaagca atgacagatt
    20641 cacgagagct tccggatcgt tctgtttggc ggcctcgtaa taccatcttt tggcttcttc
    20701 catgtcaata tccgagccgc tttgcagtct gcaggcgtct cccagggcga tcattgcttt
    20761 cacgttgcca agattagccg ctttcaaaag ctcagctttc cctgattcca cgtttttgcc
    20821 cattcccaac cctttgagca gacatacgcc caacccgtag tgaccgatga cattgtcata
    20881 agcttgaagc agacggaagt attcggcggc cttttccgga ttttccggag ttatcatcgt
    20941 accttcgaga taatcctgcg ccactttttc cagcgccttt tcgtctccga tacgggcttt
    21001 cttctctaaa taatagaaca tcatttcttt tctgaaagct tgtgaacgtt ttttaaaata
    21061 gggatgtggt ttttcataca aagaatagat tgcatcgatc gcttctttat ggttagcctc
    21121 gacagcttcg tccagccatt tcatggcttc ttcaggattt ttctgtcctt ttcccgtttc
    21181 gagcatcatc aaagccagcc tgtacattcc ttctgggttt ttgagatcgg cagatttctg
    21241 gtaatattcc ctggccttgc gaatatcctt ttcacatccg atgcctttaa gataatagcc
    21301 agccagttgg ttgtatgcgt cggtgtcctg cagtttggcg ccttccatgg tgtaacggaa
    21361 agctttcttc ttgcttttga gcattccttt gcccttcaaa gccatatcgg caagcaccag
    21421 atgcgccttg gaatatccgg aagcaagtga tttttgaaga taagaaagcg cctgcttgta
    21481 ttcctgtttt tcaatgaaat aacggccgac attgtaaaga ccgaccggat ttccctcgtc
    21541 ggctgtctgt ttgtagtatg tgtatgccag ttcgatgttc tgcggaacac cttttccgta
    21601 aaagtatttg tctccgagat cgttagtctc tttcggactc aggttggttg tcatatcaat
    21661 accccctgag gccgaaaagt tcagatttgg cctcttctga tccgagtttc gccgcttttt
    21721 cgtaccattc tccggctttg atataggaag acgggacccc gtctccgttt tcgttacggt
    21781 gtcctaacat gatcatcgcc gtgacatcac cggcatcggc ggctttcaga agataaccat
    21841 acgatttctt ggacgatttc tccacgcctt tgcctttctc ataagcttcg gccaaatatc
    21901 tgtatccttc cgtaattcct tcacgggcgg ttatttcgaa atatatgatg gatttttccg
    21961 gattatccga catgtaatat tcttttgcgg cagtatacaa ggtttttgtt tctctgatat
    22021 cggatacgct tttcgtaccg atgagccgga attggttcat ggcgtcgatc gaacctttcg
    22081 agagagcttt gttgaaccag gaatacgccg attccaggct tttccgaact ccgtcgccat
    22141 caaaataggc gagtccgatc ttatacatgg caaaaggatt gtcttttttg gcaagttcga
    22201 catatatttc cagtgctctc gctttgcttt tctggacaat tttgccttct tcaaagaatc
    22261 ttgcttcggc aagcaaggcg tccgcgacat ccttatgggc ggcggtcaca taataatcat
    22321 atgccttctg aaggtttttc atgactccca aaccttcttc ataacaataa cccatcatgt
    22381 atatggcttc tgggtacagt tcaacggctg ccttttcaaa acattcgtag gcgcgttcgt
    22441 aattctgttt ttctccgtaa ccgtaaaaat acattaatcc caaatgatgc caggcgacct
    22501 tagagcccat tttggacaat ttgcgataaa gcgacaaggc ttccgagtat tgtttggcgt
    22561 catatttcgc caaagccctt gcctcaagtt ccgaattttc caagtcaacc gcctcctttc
    22621 ccaaataata attatatcat accaaaagat tatttcgcaa aaaagccgca atgatttcca
    22681 aatgtagcaa actttttttc agccccggtt cacgattcct ttcgagccag acatttccgt
    22741 atgttttcat taccgattct atcaaagcca aagtctgctt tttgtcactt ttgatgccgt
    22801 tggcaatttg attcatggaa acgccgattt cgatcaaccg gcaggtttgg acgatctcct
    22861 ttgccacaag acattcttcg ttacaaaaga ctgagtcttc gattctcttg gcgagagata
    22921 atatcttttc cgcattatcc ggactgatct tctgttttgt aagcgcgtct tgccaaattg
    22981 cgtattttag ttcgagcgga tgactttcat ccggatctac aaacatccat gaggcgaatg
    23041 tcattgtccc gttcggaata ttgaagtcct ccagcatgtg gtatttggat aattcaataa
    23101 ttttatctgc aaagccattt gatttatcgt tgaaaacgtt ctgatcaagc catttctccg
    23161 ttttctcgat atcttcggct tctccccatg atgccgtagc ggcaaagagc aacgaaggga
    23221 gcgaaaacga caaagattgc ggatgagaat aatcgcccca atcagtcaga agaattccca
    23281 gtcccatgtg ggatttggcg gccattgctg cgtttctgat tgaatcgcgc atatcctcgt
    23341 atcttcccga aaaactgttc caagaggagg tccccggggc gagcagaaaa tcctttccgg
    23401 ctttttccat tcttatggaa ggcgtttcaa aatcgtgttc gctgtcatat ccccagtcgc
    23461 aaacgacaac atcgtttccg accagttcgt atagcgaggg atcctcattg aaaacatcgc
    23521 cccagattat cggacgtttg cccttttccc tcactctgtt catgactttc tccatgtatg
    23581 tcaaatatac tttcccgcgt cctttttcgg cgcatttttc cttgcttttt ccaagaccta
    23641 attcaaaagg ctcgtctccg ttgacattga aaaaggcgga ttgggaattt ccacaggcgt
    23701 catcgatcat ttttttgact agttcgatgc ttctaggatc aagcggattc aaggtcgaag
    23761 ccggaaaggg atagccgtaa ttgacataac cttcctcgca ttccgccata ttgtggaaaa
    23821 aatcaaggcc cagccatgcc gtcatatggc cgaaagtgtt gatatttctg acaaaatcga
    23881 ttccccttgc cagacagtaa gattccagtt catccatttc ttttggcgtg agcggatcct
    23941 cgtaaggaag atttggaaat gatttcaagt aaacggggaa tccctcgaca tacaattcga
    24001 aatggttcat tttcgatatg aaaagcatgt caatgacttc tttaagcgtc ttcatggtcg
    24061 ggattttatc ccggctgatg tccaaaagaa aaccgcgaat tccgagatcc ggccaatcgt
    24121 cgattcgctg acatctgact ttgccttctc tttgcaactg gatgtaggtg gaaagccctc
    24181 tcagggctcc cgagtatttc gtataacgga tttggctttt ctttttgccg atcgtgagaa
    24241 tgtattcctc gtcttttagt cctggctcat aagcaaattc aagatccgct tccttacttg
    24301 cattttcaat attcaagtat ttgctggcga cttttccgat gctttccgtc aagatgtcgc
    24361 aagcgatcac ggttgtttgc ggaaatacat attctccttc gataatttca atgctttttg
    24421 gtcttggtaa tatcatttcc atttttcaat cgacctttct tatggcaccc gtccaaagcg
    24481 aacaaagata ggcactcgtt ggtttgttcg tcattttacg aatgatgttt gcatatccaa
    24541 agatttgttc ggtgtattcg cttttatcga tgtcgccgga tttgtaatcg acgacgatcg
    24601 cttctttatc gcttaacgcc aataaatcga taacgccgtt tttcgcgatg ccgtcaatct
    24661 cttcatacac gggatattca cggtaaatcc tcattttgcg gatcttgtca aacggctctt
    24721 tttgaagcaa tttgattatt ctgtcttttg cattatccgg aatgttttca agcgaatttt
    24781 caggatgatt aaagtctacg tattccatcg cctcgtgaag caaatttccg tatgccaaag
    24841 cgtttttttc ttcttctttc aataagattc ccgctgattc cttggaaaac cgttcgccaa
    24901 tcagatcttt tttctcccat gtcaaattca gataaatcac cggttttgcg gcgaacactt
    24961 ttaccggttt ctccgccatt tccggaatat ttatcaaagc ttcctctttt ttttcaggga
    25021 tatgaggcag gagggaaaac aatcgggagt atttgtttat actctctcgt gtgtagctat
    25081 cgagacatcc gttatccatg aaggatgctt ccgcctcttc tttgtttatt tccggcaaca
    25141 caatgatttg ttgctcccgg gaacgcgtca gggccacata tagaaggcgt atcctttcgg
    25201 agacatattc ctttctcgcc cttcttaata gcagtaatcc gacaatattg ggtttgaacc
    25261 cgtccgacca tgtttttgtc gaaagtccgt attttcggtc gaaaacaaac aggtttttgt
    25321 tttcggtaaa attgaatttc ttgtcgaggc cgacataata gcagacagga tattccaaac
    25381 ctttcgattt atgcatggat aaaaccttca ccatgtcgga agtatcggcc gccacgttct
    25441 cgtaagcgat gtcggcttct tcttttaatc cgacagaatt caaataatcc aagaatccga
    25501 cgaatccgat gttttggagt tccaaagctt tcatgtaaag atagtccagt cttttttcac
    25561 acgcttcagg atcttttagg cggatgatcg actcgtatgc cccggtgact ttgcaaatca
    25621 tggatacgat ttccggcaaa ggaataatat tgatcacact ggccacattc ttgatatcgt
    25681 gagccaattt cgcaaacgcc ttgttatctt tcagttttat aaaaccgtca atattttcca
    25741 atccctcgca tttgaggaaa caaacgatgt catcgtcttt gatccggtat gcgaacgacc
    25801 tggccagaga aacgaccgat ctagccaaag aatgatcaag aaaatcattg ttttgaagac
    25861 aaaaggcggc ctgcatgaat tgccatacca tcatgatttc ttcggtcata atgaatgatt
    25921 cttctgtgta cactttgaca ggaattccca tatcggaaaa tgtttttgcg ataatgtcga
    25981 atccggtttt tctgtcggca atcacggcta tgtcgccgta tttaagggac ctttccgtca
    26041 tggtttccct gtccaatgtc gtttctctgt ttttcaattt tccaaaaatg tcgcaagcta
    26101 cggccaatgc ctctgttttc gtcttgtctt cgtcatccgg acgcaattcg ggaatgtaga
    26161 ccaacttttt tgcccctgcg gccaaaattg aatgcttctc ataatttttt tgtgcataga
    26221 tcattgactg gttgtggaaa tagtcgatgc cgccgatttc ctcgtccatc accggcatga
    26281 agaaatcgtt gatgtattcg agcacttccg gacgagaacg gaaattttcc ctgagatcga
    26341 tcagaattcc gccggaacct ttttcatagg catggtattt agccagaaat attcccggat
    26401 tggcattcct gaacccgtaa atcgattgtt tgatgtcgcc taccatgaac agattgttcc
    26461 tggaaattag ttgtaacaga tattcctgaa tatcgttagt atcctgatat tcatcgacca
    26521 ttatttcatt gatttcacct gacagcctat ctcttaattc aggattgtcc tcaaagagtt
    26581 tgatcgcaaa acgcatgata tcaccataat ggtacatttc tttttcccgc attgcgtttt
    26641 ccatttttaa aagaaatgca tctgccagtc tcaatatggt aagtacggtt tcctttgtct
    26701 cctggatatc ttcaatcaga gtttctttgg aagatgcttg taaggatttc acagtctttg
    26761 caataccatc atagattgtc ttggcccggc tgtgcgcgct cttgatctca tccaatgtgt
    26821 catcctcgat ctttgaggat ttcgtcagag atctgggaaa tgattcattt cttagaaagt
    26881 cgattttttc ttccagcgtg ttcgcttctt tgatgcgatg gtaaaagttc ttcatcctaa
    26941 ggaaatattc gtttattacc gggtttgaag tatcgtaaaa gacacttgat ccggcagccg
    27001 tcgaagcaat cattctgttt ccgatataat cgttgaattc tttcaaaata ttcccgatac
    27061 tttcagaaga aaaatgatcg ttttcataat tatctaaata tctatgataa tcgggcaggg
    27121 tttcaagacc aaacgaagcg acatttatcg cttctttcag cttgtcatcg ccgcgatcaa
    27181 acagtctttc cacaaccctg acgaatccgg gatccttcat tatgtagaat tcatccagag
    27241 tttcgtttag gcatttctcc cgcaaagctt cgagcatcgc ctcgtcggcg atatcgattt
    27301 tcgaaggaag attgagcata taatggtatt ttctgacaat cgacagtgca aaagcgtcga
    27361 aagtggaaat cgtcgcattt tcgattcttg attctatggg ttttaagtct ttgctcgctg
    27421 acacgctttt tctgattctc gccttcattt cctcggcggc caggcgtgtg aaggtcagaa
    27481 tgacaagctt gtcgatatct attccgtctg ttagttttct cataacgcga agcgacagga
    27541 cttttgtctt tcccgatccg gctccggcgg cgaccagaat gttttctccc tcgtgcatga
    27601 cggctttctt ctgcatcgcg gtcaggttgt ataggtcaat cgtcatcgtc agatccctcg
    27661 ctttccaaaa gctcgtcttt tcccgcggct tccagatggc aaagcggccg gttctggcaa
    27721 taccggcacg cggccgaaag accataagcg actttcttca tttggggaat gattgtgaac
    27781 tttccttcgt cgatcgcttc ggtcatggca tttatgtgct tttccatggt cttcatcaga
    27841 ttatccatat attcggttcc gacagttcgc ttaccgtctc tgatgcctcc cgacgcattc
    27901 aggctcactc cccttatcca tgaaatgtcg gaaaagcttt caattatttc catgtcggaa
    27961 atgatccttc cgtcgagttc catctttttt ttgtacgaat ccttcccttc tgattttgaa
    28021 taaagagaag gtagtatctt ctggtagaaa aacccgaacg gaatttttcg ctcgctgctg
    28081 gcagaatcat tttccagata cagataaaaa ggcaactgca cgtcagtgcc tttttcgaag
    28141 tcatcctctt tgaacgaatt gtttccggat ttgaaatcga taatcacata atagtctttt
    28201 ccttcgatgc ttttcgtcat cacccggtcg attttcccgt ggatgacata tccggataaa
    28261 ttttccgggt caagggacac gtttttttct cttccggata actcgaattc gctttgcccc
    28321 gtgaatatat caagcatgga taacgttttt tctatctgtt tacgaaaaca ttccatgaaa
    28381 atttctttcc tggctgacaa atcatcggga aatttttcca tgtcttttaa atcgattgtc
    28441 ccgttggcaa tcacggcgtg cgctagcgaa ccgaaaaaag ccttgatgtt ttctttttcc
    28501 ggttccagtc tcagaatatt tccaatcagg aatcggtatg gacacccgaa gaaaacattc
    28561 attcccgaag gagatattcc gattgttttt tccttgatct tttcgatcgt gtgttttcgc
    28621 aaacctgaga atttgggatc gtatccaatg attttgccaa caaattcttt ttcataaacg
    28681 cccgatagag cgcttttgtt tccgtaagtt gtttctttgt ctctgagttt tgcgtattcc
    28741 agaatgtcat gtcggcggga aaatgacatt cccgggatga tcggtcccgc cgattcacgg
    28801 attttccggt ttccaagagc aaattccggt tttctggttt caatgccgct tgtcaacttg
    28861 gacatgaaca aagtcaagtt gggaacccgt ctaaagatct gttctaaatg ggcaaaccgt
    28921 tcctcggttt ttttgtatgc ggttttcatc ccgatcgctt ccaagacatt gtcatcgaga
    28981 taacgatttc ctttaattgc caataaagcg tccatgtctg cgcttaagat gagattgaac
    29041 gtattttcat cgtaaccgat atgatcgata ttggcgaaaa cgatcgcgtc ggtcatcttt
    29101 gtttcccggt atttcctttt ttccgtttca aaatgcaaaa gttctggatt ttcatccaga
    29161 ttctcgcatc cgtaatcatt tagcaaatcg gccaggcagt taatgacgga atttcccggt
    29221 ttttctttta ttgtttggaa tgcagaccat gttccgaccg tttttgaaag catgacgaat
    29281 tcctgggcga tcggatgtcc ggaaagagtt ctcccccgag tatcatagaa aggcatttcc
    29341 gccatttcaa gctttctctt taatctgtat acatcctctt tgcttccacc caaaaccctg
    29401 atagttcccg gttcaattcc ggatgctaac attttcgtta tgcattgata acagttctcg
    29461 catttctcta tatcgtcggc gctttcgaat agatcgatgt cgttatggca ttctttgccc
    29521 aaataaacga cttcgaaagg aacattgaac ggcaaacagt ccaaagctat tttgtttacg
    29581 acaattatcc gtttgatatc atccgatgga atcagacgtt ttgatatcaa attgttttct
    29641 tcgaggaatt tccggatttc taatacaagc agagctaatc caacgtattc ctttttgata
    29701 tcgatatgcg ctatcgcttc cttgaggcct tccgccaaat ccggactgat cccgaaatgt
    29761 ctgcttacag gtaaaagcca gtctttcgaa aagattggct cgaaatatcc tatgtctttg
    29821 atccgggcgg gaaaaagccc gtattcgtcc ctgatttcgg atacgatcct ttcacgatca
    29881 gaaaaagcgc acaggacgag gtctttgcaa gataagccga tatccgtaag cgttcgctta
    29941 tccatcattc atcccttcct ttcccggtat ccgtatatat aacataatga tatcagaaac
    30001 atcccaaaac ataaagggat aaaatgaaaa aaagcgcctg cgtcggcgcc tttatggcaa
    30061 gtaaaaaggc ttctctcagg aagccttttc cttattcgat cgagatgtac tttttctcct
    30121 tgacgttggt tccttcctta ggaatgaata ctgtcaggat tcctttgtcg taacttgccc
    30181 tgatgtcttt ttcagcaatg tcgtcaccga catagaaact tcttgcggag cttcccagga
    30241 atctctccct gcggatgaaa tgacccttgg catctttgtt ttctttgtct tcttcaatct
    30301 tggcttcgat gttgagatat cccttttcca ggcttatctt gatgtcgtcc ttaccaaatc
    30361 ccggaacatc aatcaccagt tcataaccgt tttccacttc cttgatatcc gtacgcatgc
    30421 attgggctgc ttgtctcatc gggggtctca cagtgaagaa atcgtcgaag aaatcatcga
    30481 ggatcgaaac gttgttgtct ttcttgatta tgctattcat agaaaatgcc tccttgtgat
    30541 atttttttgt tagcactcta tattcccaag tgctaattat attatatcac ttctgttagc
    30601 actgtcaagc attgagtgcc aataaatgtg atttgcaaac gttttcattg atgatacatg
    30661 ccattcccgg tttaaaaagg tgtacaatac ataaaaaaaa ccggctaagc cggtttatga
    30721 caacattatt attgtcggaa aaaacataag aaggatacca attgcgatca tgatggctcc
    30781 gcccacaagt tgcgacactt tcccaaaacg attcgatatt cccttgactt ccaaagtaat
    30841 catcgagatg acaaacactg cgatgtcgtc taccaaaaag aaaaacacat atatgccgat
    30901 atacatcaga tatatcgcgt aaggaagact gcgataagca aggatctggg tgaaaagcaa
    30961 gggaaggccg gccgaacatg ccagttcgag aaagttaacc gctatagcca caagcattat
    31021 cccgaagatc gccatcaata tgttcttttt ggcaacggaa cttctcacca tatcctgaaa
    31081 ttttcgcttc attttcacat ttgtcacgtc gcatccggtt tctttatgtt ttaaggatat
    31141 gacgaatctt gtaatattgt agataccaaa cgcaagggca aacgaagcaa tcgcaatccg
    31201 tacccatttg atggcggcga tgctgattcc gatctttagc catgtcgtca taatcaaaaa
    31261 atagaatatt ccgcttgtca aaagaaaagc aataccgagt gtccagcgtc ttcttttgtc
    31321 cggtaattcg acaagaagtg taatcagaaa caacaatatc cacattgcac agggattgaa
    31381 tccatcgaca agcccgatga caatcgcggc aacgattagc gaaaacgaat cgactgacac
    31441 gtttccaaaa atcggaatat agacatagtc tccccaggaa aaacggagat attcgatatc
    31501 atccctgtcc acggtttcgc cattgattat cttttctgtg acatccacat aatcagtcgc
    31561 tgtcgtgtac ctttcaatca gactaacgat gtccgtctcg gtctgttcag agaatccggt
    31621 cagagcgact ccgccgatga ctagcaaggg agtcagagac atgcttttcc caaatgtttc
    31681 cctgacgcta tcatagagag ccagattgta atcggattcg gaaagctcgt aacgattgac
    31741 gacgatatca ggatattcgc tttcaagaag cgaaagatag gcatcttctt cctgacaatg
    31801 tccacaggtg atatcataaa aaaaataaag attgaccggc gatgaagcgg ataccttcac
    31861 agtatagaaa acaaatgaag caatgataag aaagaaaaaa acgaggacat gcgtgatttt
    31921 tttcatttcg gattttcctc catgaatttt cccaactcgc tgacggactt ctcaccaaca
    31981 aagcggaatg tctcacgacc gtctttttca aatattatga taggcaagat tttcgcatca
    32041 gaaaattccg ggattttctc cctgtccata tcgaaatcat attccaccaa tgcgatttcc
    32101 gggtggattt tgattagttt tcggattctc gatctcataa taaggcagct ggggcaccac
    32161 atcgcgccga tcatttttat ctgcatgatt caatctccat tgccagcacg tccagaaatg
    32221 ttcggatatc cctgtcactt gtgagatgcg aaatggagac ccttatcgat gattcggccc
    32281 tgtccatgtc gccaaacaaa agattaatca gtctcgaata cgttttttcg aaacaacaag
    32341 ccgattgcgt ggaaacgaaa ataccgcggt cagacaacct tttctgaagc actggcgctt
    32401 tgagcaaagg aaaactgaaa ttgattatct ggccgatagc gtcatctgga ctattaagaa
    32461 gaacgccatc cagggattcc agtccttcga ttaaaatacg gttcagtttg gctatcttct
    32521 tctctttaag caaaaatttg ctgtaagcat cctctaatgc cgccgcaaac gaaacaatca
    32581 gcggttcggg cggagttccc ggccggtacg cggaaacact ggcgccaccg tagagaatcg
    32641 gctccagcac cgttttatcg tttcttacca gacatccgat tcctttgaga ccgtaaattt
    32701 tgtgccctga aaaagtgaaa aggtccggat aatatttcgg cagtctgatt ttcccgatcg
    32761 cctgggtcat atcgctgtgg aaagtgactt gagagtattt tttcagtatg tttccgatcc
    32821 tttcgatatc gttgacggaa ccgatctcgc tattcacgtt cactaccgat accaagatcg
    32881 tatccggcct taccaaactt tccagttctt ctggatcgat ccttccgtct ttttttgctt
    32941 cgataatgtc cacttcgtaa cccattttga caagccggga aaaacatgcg gtgaccgatg
    33001 gatgttcctc cgaggtcgtg atgacgtgct ttccctgttt ttgttttttt atagccgttc
    33061 ccatgatcgc catgttgttc gcttcggtag ctccggaagt atagattatt tcacagtttt
    33121 cattcccgag gatctttttg attttttttg tcgatgactc tatttccaat ctggcttcca
    33181 ttccgaaagc atgcaaagaa ttcggattgc cataacggcg ctccgattcc ctttcaaagc
    33241 aaacgatcgc ctttttacaa ggcggagtgg tagcggaata atcgagatat atcaaaaaaa
    33301 atcacctcgg ttttaatcag ttcaaaaaat gaatggcttc tttggcaagg ccgagatatg
    33361 cttcatgaat gatttcggat atggtcggat gcgcgaaaat agttttcttg atatcttcca
    33421 ccgaaaggtt gttggtcatc gccacatcca gagaagcgat cagatgttcg gcatcgttac
    33481 cgaaaatctg ggctccgtag agttcgtttc cttcgctttt ggccagcagt ttgataaatc
    33541 cggcctgact atcgctgatt aaggcttttc cattggcgga ataaggtacc cgaacaactc
    33601 gatactcggt tccttctgcc tgcaattcag cttcggattt tccgatagtc gcaatctgcg
    33661 gcgatgtgaa tatcacgctc gggacatggg tataatccat tttagcatct ttcccgagga
    33721 tgttttccac tgctatcatt ccctggtggc tagccacatg ggccagttgc attttattgt
    33781 ttacgtctcc gatggcataa atactctcga cggatgtttt catccggtcg tccaccgtta
    33841 tttctccgcg ctttccgatt ttaatagaag tgttttccaa acctagcatg ttggtttccg
    33901 gtcctcttcc caccgcttcc aataccaaat cggcttcgac cgaacgcatc tcgccgttcg
    33961 tttcaaaaca aactaaggct tttcccgatt cgcttttttt tacttcggtg accttggcgc
    34021 ttgtggtgat atgaatattc gacatcttgg cataacgaag gagacgcata gacaaatcgc
    34081 ggtctaccat cggtaaaatg ctaggcaaaa attcgattac ttccactttc accccgcatc
    34141 ttccgtaagc gaaagcgaat tccattccga taatgccgcc gccgataacc gtcaaggtct
    34201 ccggtagttt tgtattttta agcaatcctt cggaatcaag atatatctcc ggagtttcta
    34261 tattgattgg aagatgtttc gtcttagatc cggtggcgat aatgatgtct ttggctgaga
    34321 atacggtctc tgtatcttcg cttttcacaa ggacacggct gtcatccaag aaacttgctg
    34381 tcccgtttat cactttgaca ccgttttttt caagcaatac gccgatgccg tctgtaagat
    34441 tcttgacaac gaaatctttt cttgccacgg cgttttcaag atcgatcgtg acttctttag
    34501 caactactcc gtacttttct ccctcttttg cctcggtaaa aacatcggct gattttacca
    34561 ggcttttggt cgggatacat ccgacattca ggcaggtgcc gccaagaaaa cgtttctcta
    34621 tcaatgtgac atccaatcct tttttggccg catagatcgc cgaaacatat cctcccggtc
    34681 cggctccgat tatcaaaaga tcagtttttc ttgtttccaa aacaatcatc ccttttatat
    34741 attattgaac ggttatccgt cttttaaaga ttataccact ttgtccgtct gttcgcaatc
    34801 tcgatagcca tttcccgagc accttgattg aattatcaag atttatttgc taatatttaa
    34861 atagaggaaa caaaggaggt ctatgtatat ggactataaa gatattaaat ttaagtccgg
    34921 cgatagcaac gagtattcac ttaacgattt ccatggaacg gggctcgtac tctacttcta
    34981 tcccaaggac aatactcctg gttgtacaac cgaagcgatt caatttactt ctcttaaaga
    35041 cgatttcgcc aaaaaagggt ataaactcgt cggcgtgtct cgcgactcgg tcgcatcgca
    35101 ttgtaatttt accgaaaaac acaatctggg aatcacgctt ttgagtgatc ccgacggaat
    35161 ccttgcggaa gcattcggag ttttaaaaga taaaacaatg ttcggacgac aatacaaagg
    35221 catcgtcagg agcaccttca tcatcaacaa agaaggcgac cttatcaaag aataccgtaa
    35281 tgtgagccct tttggacacg cagtgaacgt cctggctgat ttataaaagg cggaagtgat
    35341 tccgcttttc ttttgcataa taattagcac tctacgcttg acagtgctaa caaatagtaa
    35401 tataatattg ttggtttaaa aaaggaagtg atatcatgaa taatcaagaa ccggaaagct
    35461 accttgacga tcccaaggtt ttggataaat tcggacgtat catcaatgaa gaagtcaaaa
    35521 gcggaaagat cgatcccgtc atcggcagag aagacgaaat ccgccggatc attaaaattt
    35581 tgagtcgtaa gacaaagaac aatcccgttc tgatcgggga acccggcgtc ggaaagaccg
    35641 ccattgtcga aggtcttgcc agacgaatcg tcgacaagga tgtcccgctt ggattacaga
    35701 acaaatcaat ctatgagttg gatctcgcca gccttttggc tggcgccaaa tatcgtggcg
    35761 aattcgaaga aagactgaaa gccgtactca aaaaaatcaa agattccaac ggcgagataa
    35821 ttcttttcat cgacgagatc catatgatag tgggggccgg acgcaccgaa ggcgcgatgg
    35881 atgcttctaa catgttaaaa ccgatgcttg cccgcggaga attgcattgc atcggagcca
    35941 ccacactcaa cgaataccgg aaatacatcg aaaaggacaa tgctctggaa agacgttttc
    36001 agaaagtatt gatcgacgaa ccgacggtcg aagacaccat cagcattatc cgcggtctca
    36061 aagaccggtt tgaagcccat cacggcgtcc atatcagcga caatgcaatt attgcggccg
    36121 ccactctttc caaccgttac atcagcgacc ggtttttacc ggacaaagcc atcgatttga
    36181 ttgatgaagc atgcgcctcg atccggatgg aaatcgattc aatgccgcaa gaactcgaca
    36241 caatcaagcg aaaaatcatg caactggaaa tcgaaaaaag agcgcttgaa aaagaatccg
    36301 atgccttaag taaggaccga cttgtcaaac tggaaagcga acttgccaaa gaacgccgct
    36361 cggaagacga gatcactaag gaatgggaaa aggaaaaaca cgaattacaa aaagtcaagg
    36421 aaatgaaagc caaactggaa aactataaaa gcgaacttca aaacgctctc aatcaaagcg
    36481 attattcgaa agccgctgaa ctccagtaca gcaaaattcc cgaactggag aaaaaaatcg
    36541 aggaagccaa atcgaccgat gcaaacaaaa tgatcagcga agtcgtcacc gaagaagaca
    36601 ttgccgcgat cgtggccaac tggacaaaga tcccggtagc caaattgctt cagggcgacc
    36661 gagataagct tcttcatctc gacgaagccc tgaagcaaag ggtaatcgga caagacgaag
    36721 cgatatcgct gatctccgat gcaatcatca gacagagagc gggtgtgaga aacgaaaacc
    36781 ggccaatcgg atcattcatg ttcctcggcc cgaccggagt cgggaaaacc gaagtggcta
    36841 aagcgcttgc cgaacaatta ttttcaagcg agaaccatat tgtcagaatc gacatgagcg
    36901 aatatatgga atcattctcg gtctcccgcc taatcggggc ccctccggga tacgtcggtt
    36961 acgatgaagg cggccagctt accgaagcca tcagacgcaa accgtactcg atcgtgctgt
    37021 tcgacgagtt cgaaaaagcc aatccggaag ttttcaacat tcttctgcaa attctggacg
    37081 acggccgttt gaccgacaat caaggaagaa ccgttgattt caagaacacg atcattatca
    37141 tgacatccaa tctcggcagc gaatatttga ttcgaggcga ccagtccggc aaggacaagg
    37201 tcatggatct cgtgaaacat tctttcaaac cggaattcat caaccggata gacgaaatcg
    37261 tcatattcaa cccgctttct tacgatgttc aggtaaaaat cgtcgacaaa atgctacaag
    37321 agttatcctt gcgtctcaag gaagaaaata tctacgtcgg tttcgacagt tcagtgaaca
    37381 aaagcgtaat cgaccaggct tacgatccgg agtacggtgc ccgcccattg aagcgttata
    37441 tccagcgcta tgtcgaaacc gcgatcgcca aatcgatcat ttctgacttg attgtaccgg
    37501 gaaaaaatta tttactgcta tatgacaaag ataccggata tgaactgaaa cctgcacaat
    37561 aatcaaaaaa gaataaacaa ataaaaaagg ttttcttccc aagggagaaa gcctttttgt
    37621 ttatcattgg ttaattacct tttccacgaa aatcctcgcc agttcgggat cgaattgttt
    37681 tccggcgcct tccaggattt cccgtctggc aacttccttg cctaaagcct ttcggtaagg
    37741 ccagtcctga atcatggccg aatatgcttc gcatatggcg atgatccgcg accggaatgg
    37801 aattgcttct tctttcaaac ctttcggata cccgtgtcca tcccatcttt cgtgatgcga
    37861 taaaacatcc agggcgatcc ggttgtatcc tacggccgag aaaagaatcc gatatccgat
    37921 ctccggatgc tgtctgattc tgataatctc cgtttcttct aactttccgt ttttctttag
    37981 aagctcatgc ccgatggcga tcttgccgat atcgtgaact tccgccaatg ttccgatcgc
    38041 agccacttct gtttcggata gtccgcattc cttcgccaat tttgtggcaa attccgtaac
    38101 cattctggca tgactttctt cctgaggata ttcgttgaac agattctctc ggatcagctc
    38161 gataacattc atatgatatt cgctcgaatt gatcagtttc tgattataca tcagcatctc
    38221 ggcatcttta tatacttcga aaatcgattc ctgagggttc tttttggcag cgattccgaa
    38281 cgacactgaa cagggcaatc cgccgatcga atccttcatc aaagcatctt tgacctcttc
    38341 cgccgctttt ctggtttttt cgcaatcgca atttgtgatc agtatgacga actcgtcgcc
    38401 gcctaccctg aaaatcagat cggtaaagtt aaaagtgctt ttaatgacat tggcgacttt
    38461 tttcaaaagg ttgtcgcctt gaagatgtcc gaaagcgtca ttggtggttt taagggcatc
    38521 gacatcggcc attacaatgg aaatgggcag attttcctcg gtatcctttt cgcgaagcac
    38581 ttcttcgaat tttctcctat tgtagatccc cgtcagaatg tcgcaatagc tcagatgctc
    38641 gatctccatt tctttttctt tacgggcggt gatatcctga ttgatcccat acatcgtttc
    38701 tggctttcct tcggcatcga atatgattgt tttacccttg tcggaaatcc atacgtagtg
    38761 tccgtcttta tgtcgcattc tcagctcgga attgtaaaac ggttctttcc cgcaaagatg
    38821 tagatcgatt ttcttcttgg atgaaacaag atcatccggg tgaaccaatt tctcccactt
    38881 tctgatattg taatcgctgg tttcttcgcg gctgtaccca agcatatcga gccacttctg
    38941 attcacggat atcttgccgg atttcacatc caattcccat gttgcggtat tggtagcctc
    39001 gatgatattg tcgtatcggt ttttttcaag catcatggta cgttcagaat cctgcgctgt
    39061 ttctctttcg aaaatgaagc tgcagatttg caagggttta tcgttactga ttgccgaaaa
    39121 cacttccgca tagaatattg tgccgtctag tctcatcaaa tcgaatccgg ttcgtggttt
    39181 gctatgcata ttatttttca cattgtccac ttgttctcgg tatacagata cgtattttgg
    39241 agcgatcaat tcggtcactt ttctgccgac aagcgcttct tccgatccgc agcggaacaa
    39301 tttgacggcc gcgtcattcg caaattgaat caaatcgtcc gaataagcta tcatgggatc
    39361 acgaaaatcc ttcatagaaa aaagcggagt gtaatatttg cggaatttag gcatttttct
    39421 tgttccgttt tcatttatac tcttgttttt cccaatagtc aaataagcag caaaacatac
    39481 aaaaaaagtg cctgcagcta taaacagact attgctcaca aatatgaatc tgaaagcgtt
    39541 agtaaaagtc ataatcggca taccatcccg agtgtcattg ccggaaaacg cctgtatcat
    39601 gcgattccat tagatatgac tatattttat taaccgcttt ttgccgtcca tcccgacaat
    39661 aacttttcta gcagggcaac gagcgttcga taaagcaaaa gaactgtgaa ataattgcat
    39721 accgccatca caatttcaaa ttctagatct gccaggaaat acggccataa aatgtcgact
    39781 ccctgcaaaa tcatctgggt tggaaaataa aaccaaccat agatgaatcc gaaagcaaaa
    39841 gcgaaaatga gaatgacaaa gaacgatttt ttggcgatca gtttgcctat aacaacaaaa
    39901 aaaagccagg caaacagcat cggtatcgta taaagcaaac tgaacgatcc catgatgaga
    39961 ttatctatga aaacatagga aaccaccagc gtgcatagca aggaccaagg caaaattgca
    40021 gcatatacaa gcaacaacac ggcggtgaac tgaatattcg gaagaaggac caggtattct
    40081 tcctgtacaa ccaagatggc gagtaagatg gctgtcaata tgatatatct tagcctggtc
    40141 cttccaagat aatgtaaact attcaagctt gaattggtaa cagtacccat cggtaatcgg
    40201 cgtatcgtct attccagtca tggaatactg cccgttgata taaaacgaaa tgtagtaagt
    40261 atcgtccgta taagcatcca aatcgccgat tcccactata taatggccaa atgtgtattc
    40321 atacgaacat gtgtcgtcgg gatttccatc cgcatcatta cagtaaacgg tgtagttttc
    40381 ctgtaataac ccgagtagag tgtcgctttc ttcataagcg atggtttttt gactgataag
    40441 tccatcgttt gcatcgtaaa gttcaaaagt gatggttccg gttagagtgt cggtacctga
    40501 agtcgtaaca ttgccgcaag ccaaaaacgc cggtagcaaa atcaaggcca gcatgatacc
    40561 cagtgctttt cttttcattt ccttacccct tattagattg tattgacgaa caggtaggtc
    40621 tcccgacttc gcttgccgtc cgatccatcc ttctcgccta agcaatggtt cattggatcc
    40681 gaaaagcttc acggttgctg gcacagtcca agattttcac ttgtgttccc tgttatacac
    40741 cttgttcatc attaatgatt atatcacttt tacggcagtt aatcacaaga tttcgcgaaa
    40801 tttgcgaata tacattcaaa tggattcata aaaaaaacct gtttaccagg tttcatattt
    40861 tttcagcata cgttttcaga ttgctgatga atctgtagat atgaattcca tcacataaag
    40921 cgtggtgggc ggatacggaa atatgtatca tggctttgcc gtcaatcata cgatatttat
    40981 cccatgatat acggggaaac gaatcaggat gcttcgtatc gctgggatgt gtaagggatg
    41041 tgaatgagaa ccagggcaga gaggaaatgt agacgatatc atcctttcct tcgacatcat
    41101 ccagactttt ccggattttc gccgcttcga tatctttttg aaccttgaag acgaattgag
    41161 cgagactgtc acaatagtca gttgtcacga agtaaaaaag atcgtcatca gacaatacgg
    41221 tgtaagacgg gcttacaata tcgtgcagga taactccgtc cggtcttatc cgataacgaa
    41281 attccgggat ttggttaaga gaagtcatca ctgcatgcaa cataacggca aaaaatgatg
    41341 ttttgcttgt ttttgaaaaa tcgacaaggg gcgtcacgtc tgcttcaaaa gtgattccga
    41401 aatggggctg atcgctatct ttgaaaagca tgaagtgttt cttccttggc caggtatcca
    41461 aatcgatttt gatcacgaca ttatccttcc ttcggaaatt tcagattatc caagactttc
    41521 atgtcttccg gacttatttc gaaactgaaa acatcaagat tgcttcttat tcttgaagac
    41581 ttagttgatt tcggcagcgg aatgaatcct ttttgcagac tccatcgcaa tgctacttgg
    41641 cccggcgttc ttccatattt tttcgccata tccgccaatt cttccgattt gaatattctt
    41701 ccggtcgcaa acggagaata cgcttccaga cgaatgccca tttccttggc atagtctacg
    41761 gtttcctgag gaatgttacc cggacagatg aatatttggt cgacatgcgg tatgaatccg
    41821 gtcgcttccg tcaaaggcgt aatatggtaa ggctggaaat tggaaacgcc gatcgaccgg
    41881 cacttacctt cttcataaag tttaataaac gctttccagg aatcgatatt ggcttctgtg
    41941 tattcggatc cgtctccctt tgtcccccaa ggtttcggag catggatcag ataaaggtcg
    42001 acatagtctg tttccagttt ttcaagcgac atttccaatt ccttcatggc gacgtcataa
    42061 cctttcagct ccgcccgcag tttggtcgtg agaaatattt cttcccttct caggccaaaa
    42121 tcacgaatcg ctctgcctac cgattcttcg ttctggtatg cggcagccgt atcgatatgg
    42181 cgatatccgg cttccaaagc cgtgaaaacc gcatcatacg cttcttttcc gtttttgatt
    42241 tgccaagttc cgaatcctaa agaaggaatc gaaattccgt tgtcgagaac gatatgtttt
    42301 ttgatattca tatgaacacc tcttatatac atttagtctt tatttattat aacacctgat
    42361 gattgcggac tgaaaatgaa atgaatgctg tttttcctct tcttacgcgt ttcattcgtt
    42421 tttttatgct atcattgata cttagagatc catataaaga aagaaggcgg aacactatat
    42481 gaacaaacct attttttccg atctggcgcc taaagccatc ggtccatact cccaagcaat
    42541 tgactgtggt gatttcttgt tcgtttccgg acaactgccg gtcgatcccg ttacgggaaa
    42601 catggaaaaa aacgacatca aaatccagac aaaaagatcc cttgacaatg tcatcgcgat
    42661 tgcaaacgaa gccggatatt tcaaaagcga tgtcgtcaag tgcacggtct atctcaaaaa
    42721 tctgaatgac ttttcccaga tgaacgaggt ttaccaggaa ttcttttcaa accatcgtcc
    42781 ggctcgggta gctttggaag ttgccagatt gccaaaagat gcgctggtgg aaatcgatgc
    42841 aatctgtcac aaataaacaa aaccccggtt gcttgttaca atcggggttt gtctcgataa
    42901 tctcttagtc caacggactg aaattgtcct ttgatacacc gcaatcgggg catacccaat
    42961 cttctggaat gtcttcgaaa gcggttcccg gagcaactcc tccctcagga tcgccgaatt
    43021 cgggatcata gacataacca cagacttcgc aaacccattt tttcataatc gaatcctccg
    43081 ttaacatgtt tccttttctt atacactaaa atgtatcata gcaccacctg attgtcaagc
    43141 gggtaccata aaaccagata agcaaaaatc agttttccaa atgccctgcc atcttgaaga
    43201 aacccggtaa aaatgttaaa atagcataca aaggcataaa aagattggag aagataataa
    43261 tgcccagcaa tgatattctg ttttccacta ttttctgcat cgttgtctcg ctgctcgctt
    43321 tcggtcttgc gatttggctc tatgtttggg tcaaaaagca ggaaagcaac aaccaaaaga
    43381 tcgattccat cggtaaattg atcaaaagcg gagcaaacac ttttctaaaa aaagaatatg
    43441 aattattggc ccggtttgcc ggagtcgtgg cgattctgat ttttgttttt ctgccaaagc
    43501 cgatctggca atcgaccggt tgggccgatt ggtcgcagaa tctgaccata acgctcgcat
    43561 atctcttcgg aaccatgttc agcgcgctcg ctggcaagat aggcattttc gtggcgacga
    43621 tcgccaaccg taaaacagcc gaagccgccc aaaagggaat caaagaaagt tttttgaccg
    43681 gatttcgtgg cggcgccgtg atgggaatgg ctgtcgttgg cgccagtctt cttggtatat
    43741 cgattgtttt tctgatcgct aatcaagacg cgacaatgct tctcggtttt agtttcgggg
    43801 cttcttccct ggcattattc gccaaagcag gcggaggcat ttataccaaa accgccgata
    43861 tcagcgccga tttggtcggt aaggtcgaac tgggaatccc ggaagacgat ccgcgtaatc
    43921 ccgctgtcat cgccgacaat gtcggagaca atgtcggaga cgtcgccgga atgggggccg
    43981 accttttcga ctcgcatgtc gcatccatag cggctgccat ggtcatcgca acgaccatca
    44041 gtactaaaat ggtttcggtc gtcttcgtat atgccggact cggactccta tcctcgatca
    44101 ttggcgtcat cttcgcaaga atggggaaaa aaggcaatcc taccacggca ctcaatctga
    44161 gttcatatat cacaatggga atattcacgg ttcttaccgc cctggccact ttaatctttc
    44221 gttttgcctg gggaatcttc ggtgcatccg tcgtcggcct cgcagtcgga gctgttgtcg
    44281 gcctagccac cgattacttc acggatgatc gcaagagtcc ggtaaaaaaa gttgccgaag
    44341 ccagcaagag cggcccggcc ctgaccatca tttccggtgt cagttacgga ttcatgtccg
    44401 tattgccagc catggtcggc atcggacttt cagcttttgt cgcctatatt cttaatccgg
    44461 actatccatt gttcggaatc gccatgtcgg ccgtcggaat gctatctgtt gtcggaatga
    44521 tcatttcgaa cgatgcttac ggtccgattg tcgacaatgc cagaggtctg gcagagatgg
    44581 gcggtcttgg cgaagaagtc atccgtagaa ccgatagcct tgattcggct ggcaatacag
    44641 tcaaagccgt caccaagggg tttgcgatcg gcgccgccgg ccttacggtc atatcattac
    44701 ttggggcttt tatcagcgag gttaacgatg ccgcaaccga acgcgggctc gccaccataa
    44761 caggatttga tatcatgaat cccgcagtct tcttcggttt gcttgtcgga gcggctgtt
//
LOCUS       DAOPJL010000005        43785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_896_length_43785_cov_7.531969, whole genome shotgun sequence.
ACCESSION   DAOPJL010000005 DAOPJL010000000
VERSION     DAOPJL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 43785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43785
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_43785_cov_7.531969"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PLP02_01345"
     CDS             complement(<1..1179)
                     /locus_tag="PLP02_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF42146.1"
                     /translation="MSTKDGDLTYFLKSNESLLRETLREFAEKSYESASLNEIIKRSS
                     FNKGSFYYRFPEKKYLYFALIDDLYTNMRIVSSEDDFILDKPVSIKNAINILFENLYR
                     IYCLNPHYLGLLCNIFLDTPEIQKEITKQCSKSFFDAFAKRFTKTIKHYDYDKTNKTY
                     FLLKEIDFHYNEAIRLLPEHPDNNDFMKLAEDTSLWLEAIAGPLISAEILKSLHEENA
                     KYVKTNEDNFQKDVVFIKQSEILGIIGENDLELGELAKDILSKDTKRTDFRIRGKIRE
                     NAFLLDTNQITEPMNGSQTVNLAIKTILKANSIHMEKKELAVYLEQFYLENISNCKLS
                     ALPPTETLFLNLFAILYKDPSMIIIQRLDQWMERYKIKRFLAILLKYRPKKCTIVLTM
                     K"
     gene            complement(1285..1782)
                     /locus_tag="PLP02_01350"
     CDS             complement(1285..1782)
                     /locus_tag="PLP02_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HPF42147.1"
                     /translation="MEIRNKTLYDKDLIIRYNHYYLMDFLKKNSLFVILTACGFGAYL
                     LVQNEWEYALAMVGILAAYFLLAIVMQMLTTKRAVKRSPIVANPVYQTYVFKEDEVEI
                     FNIKARTIKYENIIRIKESKEFLIVYDLEKKTFIVDKNGFEADNDLNILKDFLKEKLG
                     KKYRK"
     gene            complement(1803..2768)
                     /locus_tag="PLP02_01355"
     CDS             complement(1803..2768)
                     /locus_tag="PLP02_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPF42148.1"
                     /translation="MPFEVEIIKWFQSFRNGFWDLFFQLWTMFGEELVIVGILGFMFW
                     CLDKKRGEAIGITVFASLIFNSALKGFVQRIRPYDADADIVNVRSSTTDKYSFPSGHT
                     QGAATTFFSLSTWMKKHWLTILSIVVVIMVMLSRMYLGAHYLTDVIAGGLLGLAFAIG
                     FYYLFQKVKNRALIYWVTAITSIVLVVASYLFYIISMPSFDVAMQTTDADYVYYGLVG
                     LFKMTGAMLGFVLGISFEKKRVNFENHRVIWKNIIRFALGVAIVMGVRYVLKYLFGFI
                     VDSSAFAWGDTFKATIALILDMIRYLAMVFIGIGVYPLLFKKVKI"
     gene            complement(2768..3589)
                     /locus_tag="PLP02_01360"
     CDS             complement(2768..3589)
                     /locus_tag="PLP02_01360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPF42149.1"
                     /translation="MMIRMFAADLDGTLLGRKSKVSERTVETINKLKKAGIFIVIASG
                     RSKEEIMSLVSDFQLDMYDKAYLLPYNGALTIKADNMDIIKRTTLSHNDLSCIIGAIH
                     DAGMFCHLYPETGLMVSDGLTEAQILGKEAMDGFTTISKNLENIPAWAYKILVFDSKE
                     KLDILRNGLPSEIEEKYGVFKSAPFLLEFSNKIATKGSAMKDLADSLGIDQDQIIAFG
                     DEENDISLLNEAGLGVAMANATDIVKKQADLIAPSNEEDGVSRILEAILEGKEEI"
     gene            complement(3596..4165)
                     /locus_tag="PLP02_01365"
     CDS             complement(3596..4165)
                     /locus_tag="PLP02_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPF42150.1"
                     /translation="MIELIRLFLSFFKVGLFTFGGGYAMIPLIEDEVINNQWLDSMDT
                     LVDFIAVSESTPGPFAINIATFIGYHEAGVFGAIFATLGVVMPSFIIIYLVAKFFTKI
                     ATNRYVQGFLSGVKPAIVGIILSVGIAFLVRNVFSTEITDISQAILDWRLLAIAGVLM
                     AFVIVFKKMNPIYLILISGALGLLVFGLM"
     gene            complement(4158..4745)
                     /locus_tag="PLP02_01370"
     CDS             complement(4158..4745)
                     /locus_tag="PLP02_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPF42151.1"
                     /translation="MPENPEKDNKAGKCLELFALFFKIGLFTFGGGYAMIPLIEAEIV
                     EKKKWISNDEFLDIIAIAESTPGPIAVNSATYIGYRKKGFLGALFATLGVIMPSFLII
                     LLISLFFLQYKENIYIQYAFKGIRVGVSILIIMAGLRLFKKLKKDIFGYIMIAVGFFI
                     ALFDLVPVLILIAASGVIGLGRTFIIMKRQVSKND"
     gene            complement(4729..5562)
                     /locus_tag="PLP02_01375"
     CDS             complement(4729..5562)
                     /locus_tag="PLP02_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF42152.1"
                     /translation="MIVFLIQIVLAAIGTAFYGLYGNYPHNGWAVLIMLACFLIILFI
                     LTVFTILWIIASIYILKGTDKKSIAKHRYYAFFIPYVFGVLLRVRIVITGKENLPKPE
                     DGNFIVVSNHMEYSDPVYIKFAYSKYNLAFVAKKELFRIPLIKTLMEGTGCISLDRES
                     TKQGLQAILDTIKAVKDGQPMAIFPEGTRSYENKMGEFRHGAFKIALKANAGISPVCL
                     YDMHGIIKIWRFGIHTCRLHILPFVPYEEIKDKSTAEVSDRIYALIDERLEIYNNAGK
                     S"
     gene            complement(5559..6035)
                     /locus_tag="PLP02_01380"
     CDS             complement(5559..6035)
                     /locus_tag="PLP02_01380"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPF42153.1"
                     /translation="MISMIFAIDKNRLIGSNNNLPWHYPEDLAYFKKTTLGHTVLMGQ
                     NTFLSILSSLGKPLPGRKSVVVSKTPFSYPDIVILDDLVTYLKGEHDEEIFVIGGKSV
                     FEQSLPYAQKLYITHINKEYEGNVYFEGLDLSLFDLIQSNKSGDLVFAIYERKQEK"
     gene            complement(6036..6914)
                     /gene="thyA"
                     /locus_tag="PLP02_01385"
     CDS             complement(6036..6914)
                     /gene="thyA"
                     /locus_tag="PLP02_01385"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPF42154.1"
                     /translation="MEQYLKFLEKILSEGIHKNDRTGVGTTSFFGYQMRFDLSEGFPL
                     LTTKKIHVKSIIHELLWFVSGDTNIRYLVQNGVRIWNEWPYEAFRKSPDYRDETMEEF
                     IGKIADDPVFALKHGDLGPVYGKQWRNFEGKDKTVDQLLEAVKSLKDNQGSRRIIISA
                     WNPAEIEKMALPPCHTLFQFNVTGNKLDCQLYQRSADAFLGVPFNIASYALLTIMMAH
                     VTGYEPGTFVHSLGDCHIYDNHREQVDVLLSRKPRKLPEMRIKRKVESIDGFCYDDFE
                     LSGYDPYPAVKGKVAV"
     gene            complement(6985..7314)
                     /locus_tag="PLP02_01390"
     CDS             complement(6985..7314)
                     /locus_tag="PLP02_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPF42155.1"
                     /translation="MLNQVILVGRLTKDPELRKLEDNRSVTTISIAVQRPFKSGETNE
                     YETDFFNCTLWSGIAEATVDYCKKGSIVGVKARLQQRDYLQDGKKMFSYPEIIVEKIS
                     FISSQKD"
     gene            complement(7497..8084)
                     /locus_tag="PLP02_01395"
     CDS             complement(7497..8084)
                     /locus_tag="PLP02_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPF42156.1"
                     /translation="MYTPVTLSLQGWQVALIVLVAVVLIIVFWYIGVMNKLRQLELKV
                     NEGESGIDVALTKRFDMLTKMLETTKGYAKHEAETLEKVVKWRSGIPESATLKDKEEF
                     LGQLNKVASGIDVVVEKYPDLKANTVFLELQSAVANTEEHLQAARRLYNANVTSINHI
                     IVTFPQSIVAKAINMDKKPYFEAEEKKRQDVEFKF"
     gene            complement(8134..9021)
                     /locus_tag="PLP02_01400"
     CDS             complement(8134..9021)
                     /locus_tag="PLP02_01400"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HPF42157.1"
                     /translation="MKSKLDVFNQTKKKSETLTLIGIMLVVLAFIIYMAIYSATASED
                     MAAIAFIVGLAGFIVGAVGLTQFNMLKRRFKKEVLKAYFSEALPGTEYYPERGLSQME
                     VYATEFLKRADRFYTEDLLTGNIDDVGFISSDVRLEERHVQHTQNGTRVYYVPYFVGR
                     VFRFTFNKPFDGYLQVLEAGSPLSNRPFKKVKLESIDFNKQFKTYATEEISAFYVLTP
                     DIMEAIMELEKRNPGRIGFSFSEKDLYIAINNNRDTFEIKMFRKIDQAMMDSFKQDIL
                     AVRDFINLLKLNKKIFKEI"
     gene            complement(9036..10523)
                     /gene="glpK"
                     /locus_tag="PLP02_01405"
     CDS             complement(9036..10523)
                     /gene="glpK"
                     /locus_tag="PLP02_01405"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016938408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPF42158.1"
                     /translation="MQKYILAIDQGTTSSRAIIFSSEGKMVCLSQKEFSQKFPQPGYV
                     QHDPMEIWESVSVCIGECLVKAKINPAQIAAIGITNQRETTVVWDKETGKPVCDAIVW
                     QSKQTTGICEKMRKRGAEPIVREKTGLLIDPYFSATKIKWILDNVEGARKAAEKGKLL
                     FGTIDSWVVWNLSGGHAHVTDYTNASRTLLFNIFDLAWDKELCDLFQVPMSMLPEVKP
                     SSGLFGKTAKNVFYGHEIPIMAIAGDQQSALFGQTCFNKGDVKNTYGTGCFLLMNTGN
                     EPVRSEKGLLTTIAWYIDGKPTYALEGSIFVAGSAVQWLRDGIKVIKNAPESETYAKN
                     IKDSGGIYFVPAFVGLGTPYWDKDARGAIFGITRGTGREEIIRATLEAIAYQTKDVLE
                     VMESESKIKLNRLRVDGGASQNDFLMQFQSDILNVYIDKPETSETTALGVAYLAGLAI
                     GFWTVEKIRAMWGVDKVFKPSITEIKRKELYDGWKKAVKACMAWK"
     gene            10629..11558
                     /locus_tag="PLP02_01410"
     CDS             10629..11558
                     /locus_tag="PLP02_01410"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF42159.1"
                     /translation="MIFTKKIISKDSQGNPIHIYLYEPQEKPVGVVMIIHGASEHFAR
                     YGLFSEYLNKSGFVVAGCDILGHGLSTDTTAYVHFSDKNGDSQAFESVLLVKDLITEK
                     YPDLPKYVFGHSMGSFLARKLCIDYPDYFQKAVWTGTGFVSKSTIIMGKVIGKLLIMF
                     HGPRYVSKFINGIAIDAYPKKMRERGIISGIDEEWLTKDKAIQDYYHNSPICGQPFTL
                     GANLAMAKWADYVNKPVNIAKMNHDLPIFIGSGQNCPLGDFGKGVTRLYELMKKQGFK
                     NVELNIYPDDRHEILNELDRDKVYKDIIDFLKK"
     gene            11639..12655
                     /locus_tag="PLP02_01415"
     CDS             11639..12655
                     /locus_tag="PLP02_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42160.1"
                     /translation="MKKVMLATTMAFALIAGLFLVGCDQATATDELSYATIEINPAVD
                     LVLMNNTVQTATALNSDGEMLLTQLSLNGLSAEDAVNDIIDEAIDLGYIDPETEGVTV
                     QVSCTNEGTQTMLQEKINTAFQERGMFGRAVAAQNADYIAEAEELGVSAGFLRLVYRA
                     MEADDTLLKEDALVMSTQDLIAIVKDNNGNVNGLSQELKDEFFAARDDLRDEYLPQIQ
                     DLQAQITAAEADGLDTTDLQAQLDALVAEFHDELALLRADYQLEGAALKAQVQSEIQA
                     RIQAHQSDVQAYRAQIRERIRTYKDEIKAYQNGSTTQTTTTTTATSGEENTNQNSTSD
                     GGSN"
     gene            complement(13232..14242)
                     /locus_tag="PLP02_01420"
     CDS             complement(13232..14242)
                     /locus_tag="PLP02_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPF42161.1"
                     /translation="MKLEERLEVKKVFSTYVDALESNDVALMESLFSENVILESTNYG
                     NANGKKQVANKISWSGEPINYSRYKIFNFVCFTENGHACQSAVVTGLVGINDDDYFHY
                     FNFGGYYLNDYVKENGKWKISHIRFNLDFEDGNTCFVRNWWKLIDYRYFEGNISYPIV
                     SEIHAPWRVIENPESLGTDEEQVLDAYYRYSWGIDHADFEIFKSCIDSEFCFGNSILS
                     NGKNQTETKEDMLANLIKFMKYKRFKEATMEHIWKIVSVDIQNGIAKVKSMRYEPNRM
                     STTKFNRLNMETDFYTGKFEYEFVKREGNDWKTGGWKMRLNNPKELKIFSETTHDKNK
                     FF"
     gene            complement(14239..14736)
                     /locus_tag="PLP02_01425"
     CDS             complement(14239..14736)
                     /locus_tag="PLP02_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HPF42162.1"
                     /translation="MLNFLHAEKKTKEHEKCFVYSPVKGHVFPIEEISDEAFSKHMLG
                     DGFAVNPVSNQIVSPVNGKITAVFPTNHAIAITAENGTEILIHIGIDTVNLNGSFFHS
                     LARMKQKVVVGMPLIMVEFAKIAEKGYEIPVIVVVTNLNEGYKIQKNIDAFDNEPVLE
                     IERGE"
     gene            complement(14730..15590)
                     /locus_tag="PLP02_01430"
     CDS             complement(14730..15590)
                     /locus_tag="PLP02_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HPF42163.1"
                     /translation="MKITRILNNNVAESQRNGRDVVVMGNGLAFGKKIGDKIEEEKIE
                     KTFELSNDTNISARIYQLIEAIPFEYWDFTFDVEKLVFDETHFVISSGFYISLLDHIY
                     TAVKRAKEGIHIPNFFTYDIRKFYEPEFVLAKKIVACMNERFNVITDENEDTFIVMHL
                     IDAQFSGDIGVMNRVIDITNKVLLIVGRFWKNKIDTESIEYERFKTHLRYFIERAFAN
                     QSEKSKIDGVETILADLGHKYPIQYACVESIKDYIQKELNILVNKDEEFYIMLHLIKI
                     MNKQEGTSLC"
     gene            complement(15770..17236)
                     /locus_tag="PLP02_01435"
     CDS             complement(15770..17236)
                     /locus_tag="PLP02_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPF42164.1"
                     /translation="MRKDFLWGGATAANQFEGAYLEDGKGLSTSDIITGGNRKEKRYP
                     TPVLQKDKTYPSHVAVDFYHHYKEDIALLGKLGIKVFRMSIAWSRIYPNGEEEYPNEN
                     GLKFYENVFKELKKYNIEPLVTINHFDMPLALCKKYHGWADRIMIDLFVKYATTLFER
                     YKNQVKYWLTFNEINTSMMDVVSDMPSILSNEQYGDSLLLQGLYYENMSDIPNESLRQ
                     RKAQAFHNQIVAAAKTVIVGRSINKKFHFGNMIAYGLYYPETTDPSDQLFAMQLEEVY
                     NYIAGDIQARGAYPAYAKRFFKEKNVEIHFGENDEKILKKGTVDFITFSYYFSNVVSA
                     NQEKMRNASNFGGMHNNYLKTTEWGYQTDPIGLRIVLNKLHDRYNKPVFVSENGLGAI
                     DKVENDGSIHDQYRIDYIRDHIKQIKEAEKDGVDVFGYAIWGIIDIVSAGTGEYRKRY
                     GLIYVNRDDEGNGNFERSEKDSYNWYKKVIETDGESLN"
     gene            complement(17312..18694)
                     /locus_tag="PLP02_01440"
     CDS             complement(17312..18694)
                     /locus_tag="PLP02_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HPF42165.1"
                     /translation="MDYKELASAIVLAVGGEDNIVNVAHCATRLRFVLKDTEKFNEEA
                     LKKIDGVINTMSFGGQYQVIIGPHAKDVFPFMPSKGKGEKKEKSDKKKGFKHVLASIA
                     EFLSASIGVAIIPLIGAGMIKAILTMLIQFGWLGTDNNTYIVLYFAADAIWYFIPVFL
                     AIGAARKLGSDPILAVLMACIMLSPTFVSYVNAGTPLTIFGLPITLTTYGQSFLPILL
                     TVYVMSLLEKALKKYIPSGAQMLLVPVLIILIMTPVALVLTGPLTNWVAILIAKPLEA
                     ISDYTWILVMILAALMPILIMFGLHGAIVTFIILTWYVTVGYDPVFMVAALCAHISIG
                     TTAIVVGIKTKNRVMRSTAYSSGVTMLIGNVSEPAIFGVLLRSKKALITTCISAGITG
                     LYAGLMGVKCYVIAGASWLFMPLFIGDGSTWFAVISTVILATILSGVLAFIFYKDPAD
                     LAKNPETENA"
     gene            complement(18882..19145)
                     /locus_tag="PLP02_01445"
     CDS             complement(18882..19145)
                     /locus_tag="PLP02_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPF42166.1"
                     /translation="MVSKDVMVECENGLHARPAASFAFAAAQFESEVTIAKSESQDLG
                     VNAKSVISVLSLGVRKNESVKLTAIGSDEEKAIRELTMFLRNN"
     gene            complement(19161..20879)
                     /gene="ptsP"
                     /locus_tag="PLP02_01450"
     CDS             complement(19161..20879)
                     /gene="ptsP"
                     /locus_tag="PLP02_01450"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPF42167.1"
                     /translation="MRKYSKGVTIVEGIAIGKASILFRDSIDTKNIAYIIGNEYEKEI
                     ALFEATRQTVLEKINELQSNMKKKNPEEAKIFDAHAEIVKDPELEKKVRLLIKQKNYT
                     CRSALLVSFKEISNMLEQIDDELIKERAKDIEDIGKMFIDTIDGDKNKNANMFYEEGT
                     ILIAEELFPSDLASVSKDYIKGIALSKGSATSHVAIMAKGLKIPTVFGITGITDMIKQ
                     GDEIIIDGYNGTIYENPDSATLDKYNDLKKNYETKQKKERDILKRIPFTTDKTRIDLA
                     VNIGSSNSYKPEIAQFTDGVGLMRTEFLFMERNDFPSEEEQFDVYRSVLQDFAGKPVV
                     LRTLDIGGDKKLNYLPLPEEENPFLGCRAIRLCFENPEIMHTQLRAAFRASAYGNLQI
                     MYPMVGSLDDWNRIQGFVEKVKKELLEKNIPFGLIKQGIMIEIPSIVMLAEEISKEVD
                     FASIGTNDLAQYLTATDRMSDLVRPYYQSFTPGLFRFIKIAIDAFHKNKKPLSVCGEL
                     GGDSRGAAILIGMGIRKLSVSPSLLANIKELVTTKSLVELEEIAENVLNFGKQDEVLD
                     YINKKL"
     gene            complement(21057..21749)
                     /gene="radC"
                     /locus_tag="PLP02_01455"
     CDS             complement(21057..21749)
                     /gene="radC"
                     /locus_tag="PLP02_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPF42168.1"
                     /translation="MYMIKDIPGSERPRERLLTIGAENLSIHELLAIVISTGSQNNSA
                     LETARDILSDLETIGELRDRTAEELMKTKGIGVSKATKLLAAVELGKRVLNENRETVP
                     IRSPADVFKLLKDEIGLLKQEVLYALYLDLKSNLIAKRKIFVGGLNQSVAHPREVFRY
                     AVKYSAYQIILAHNHPSGDPEPSANDIEMTKRFLEAGEILQIFLADHVIIGKAGYVSI
                     VDKLKNGRIRKN"
     gene            complement(21826..23091)
                     /locus_tag="PLP02_01460"
     CDS             complement(21826..23091)
                     /locus_tag="PLP02_01460"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPF42169.1"
                     /translation="MFNNFTEAREWIETQKRFGEKLDLSKMRLACAKLNHPENSFRSV
                     HVAGTNGKGSTVNYIKNILDDAGFKVGVYTSPFVLKFNERIGIGQEFISDEDVVKYAS
                     FLRNFRDRLLTEDNEALTFFEIITLMAFMYFRDQKVDFAVIEVGLGGTLDATNVITPE
                     VSCITNISYDHMKQLGNTLESIALNKLGIVKDGIPLVTSIENKSLFPLFSEVVAKNNT
                     RMKIVDLSLISNVKIGEETTFMYRTEEYRLKMAGMHQVKNAVLAIEAIRILRLKDNLT
                     ITEDNIRNGLAVTSWPGRFEIFEHQIVLDGAHNIGAVLSLKKALADIFPDRYIICLFC
                     MMKDKEHEKVIQELDNVVDEFHFAEIEYPRRADAETLFQESHHHNKFLHHDAKAAFLE
                     LREHLKENQILLVTGSLYLVSDIRPLIAK"
     gene            complement(23095..25707)
                     /locus_tag="PLP02_01465"
     CDS             complement(23095..25707)
                     /locus_tag="PLP02_01465"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPF42170.1"
                     /translation="MRARKPWWKGRNTMNDMNQKYDHLEVEKGKYAFWLANHFFEAGN
                     MNKPPFCIVIPPPNVTGKLHLGHTFENTLQDIIIRRKRMQGYDALYLPGMDHAGIATQ
                     AKVDQKLKMEGISRFDIGREEYLKVAWKWKEEYAGYIRKQWESMGLSLDYSRERFTID
                     KGLSEAVEKVFVTLYEQGYIYRGVRIINWDVEAKTALSDIEIEHKEIQGNLYYYTYKF
                     SDSDGGLTVATTRPETMFADTALMVHPEDERFRQYIGREVYIPLTNTKIKVISDMLVD
                     REFGTGIVKVTPAHDPNDFLVGQRHNLPMPLCMNEDGSMNSLAGPYEGMDRFQCRKEV
                     VKELEKQGLMQKIEPIIHNVGHSERTGIIIEPRLSKQWFVAMKDLADKALENSEVAFV
                     PERFQKIFEHWMEDIQDWCISRQLWWGHRIPVWYKDDEIYCGHESPGEGWRQDEDVLD
                     TWFSSALWPFSTLGWPHETADFKRYYPNSVMVTGYDIIFFWVARMIFQGIHFTKQSPF
                     KECVIHGLVRDSEGKKMSKSSNNGVDPQDVVREYGADSLRYFISTNSAPGFDLRYETE
                     KVESSWNFINKLWNISRYVIMTTDGMKKEDIVVKSDSLGFAEKWILERLAETIKEADR
                     QFDRFDFGEAAKAIYNFTWNDFAAWYLEMSKLNQDSATTKSVLLYVLDAIIKLLHPFM
                     PFVTEDIYQRIPHDILSITIAKWPTAEYSYPETSGKEYFFELIRKVRQIRNEYNVPYT
                     KKIAINIECDPEVRTFTQENSQYIERFLNPEKIVFTQKAENHKRAIMISMPELIAFIP
                     LGSLVDIDAEISRLNKETDRLQKEIDRSSAMLTNESFLAKAPEAKIAQEKEKHENYRK
                     QLAETKARLQELTE"
     gene            complement(25661..26140)
                     /locus_tag="PLP02_01470"
     CDS             complement(25661..26140)
                     /locus_tag="PLP02_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF42171.1"
                     /translation="MDNYIKYLRNMVGDNKVILCACSVVIVNEKNEVLLEKRSDNHLW
                     GLPGGLMELEESIEECAIREVYEETFLKIRLTRFLGIFNNPMMRWREHDEARVIAFAF
                     AAKIVGGSLRVNDHESLEMQYFSKDKLPRIHSVDNYETINAYYAGKEALVEGKEYHE"
     misc_binding    complement(26177..26383)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(27054..27656)
                     /gene="yihA"
                     /locus_tag="PLP02_01475"
     CDS             complement(27054..27656)
                     /gene="yihA"
                     /locus_tag="PLP02_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPF42172.1"
                     /translation="MIIRSIELAATAALKKQYPDGDLPEILFAGRSNVGKSSFINSLL
                     NNRHMARVSQTPGKTRLVNFFLINKEFHLVDLPGYGFALGSREDVETFRQRVADYLNS
                     KRNVVLALFLLDIRHKPSKDDIVMRDFFNAKGLPVCYVLTKSDKLSNNERSKSLKLIK
                     ETIGDVNGTYVPFSAKTGENKELIWNIIEKSIAKEVGLND"
     gene            complement(27671..28849)
                     /locus_tag="PLP02_01480"
     CDS             complement(27671..28849)
                     /locus_tag="PLP02_01480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPF42173.1"
                     /translation="MNECKVYLNKGEDERIRRGHVWVYSNEVNRITGPIESGNLSDVY
                     TFSGDYLGQGFLNTASKIFVRILSRNPDDVIDNVFFRKRLEAAKTHREDAGLTDTYRA
                     CFGEADGIPGLIVDKYSDYLSVQFLTLGTDKRKADFVQLLNDIYNPKGIYERSDVKAR
                     EKEGLEQIKNLLLGVVPDRVMIKEGNIKLYVDIKNGQKTGTYLDQRNNHLSIIPFVKD
                     KSVLDCFSHDGNFALQAKLAGACRATAVDISQDACQRISENAKLNNLDIDIVKSDVFE
                     YLRMANRQKAKYDVIILDPPAFAKKKSDVEQASKGYKEINMQAMDLLNDGGYLVTSSC
                     SHYLELNHFLDILAEAAADRKKNVQMVKLSLQNSDHPVLLGSDETMYLKFLILRILSY
                     "
     gene            complement(28868..30145)
                     /locus_tag="PLP02_01485"
     CDS             complement(28868..30145)
                     /locus_tag="PLP02_01485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPF42174.1"
                     /translation="MTINNATWLSGEKMLDTRLTNGLRVVVLPKKGFFRKAVTLIIPF
                     GSEDENIECQDEKMSFPAGSAHLLEHLLFESQDGFYTRKFTEIGSTANAYTTNNRTAI
                     YFSTVGAIDEPLKLLFEMLENVDFRKDAINKEKKIVSEEISLYDDDVEQSLYFDLMRS
                     LYRSNPVKNDIAGSKESLRTISAGTLEKAFSCFYRYQNMILVIAGDVDKEAIITLLEN
                     LASDNVLVNDHFKRIRKIDPEGLAREYYTEKRDLRNSIVMMGIKLENSVLRTPMEAAL
                     EEIKGYLFLENYMGKMSGRELELQKQGLVNDTFDFSLSVTTNYGHIILYSETKKPEET
                     MEAFRNLFALTREHIVKPDDLERQKRKMIGDFVLAFDSVSRSCSLLSEYLARGADIFV
                     IMDAINRMTPKDLQETGNKYALMPQAFVHYGVR"
     gene            complement(30142..31413)
                     /locus_tag="PLP02_01490"
     CDS             complement(30142..31413)
                     /locus_tag="PLP02_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPF42175.1"
                     /translation="MFKIATNPGQTRIRDIHTDKFKGVQISLCFIGDFKENNLNERAL
                     LPEILTGGNKRLKDRTMIQRELGSLYGAEIVTSTRKIGFQSVISFDLIFPSEKHLPGN
                     PVIFPQALSLLKELVFQPDFGGCDYFKDLFKLEKRLLRESLENDYHDRFLYGYDCFRK
                     RMFPKELFRFSARGELETIDNLTGESLVKAYHDMIDNDRIMIYSIGDFDRKYLNILED
                     LEKYGNNDSLLRPIDDEGTLPDIPTESEDLADVHQSRIYMGYRTKIVPGHPLYHALIV
                     ANVLLGDSDQSSLFLELREKRHLGYDVFSNYIGDKGVLFVFAAIEPGKEIEAKELAKR
                     IVSGMKDREISEKDLEMAKEVIVKRIHQAADSPSSTVNRIFFYDLFYNKEYDMQSSIE
                     DIRRVSKEDVRQAYSSLFLDTVYVNKRRGIL"
     gene            complement(31468..32172)
                     /gene="deoD"
                     /locus_tag="PLP02_01495"
     CDS             complement(31468..32172)
                     /gene="deoD"
                     /locus_tag="PLP02_01495"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPF42176.1"
                     /translation="MPTPHIEARLQDIARTVLMPGDPLRAKFIADKFLSDVRIVNGVR
                     NMLGFTGKYNGKEITVMGSGMGMPSIGIYSYELFKFYNVERIIRIGSAGAYSPKLKLF
                     DTLLVTEAWSDSNFANAQNGYKKHILKSSPELNLKLIENANRLGISLAQGRIHSSDVF
                     YRENPDIYLDFVNKHKCLAVEMEAFALFHVATVLDKQAACLLTISDSFVTKQETTAGE
                     RETAFTNMMKVALELE"
     gene            complement(32172..32771)
                     /locus_tag="PLP02_01500"
     CDS             complement(32172..32771)
                     /locus_tag="PLP02_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42177.1"
                     /translation="MEDGRQKALMDSAIKLASPIKYTMNITNTVLFAVILYVMDRIFE
                     FGIDIYTILIFFGLLLIYMFARDLMMNFQAKKKVFSQYQFLLKNKPGMELYVPVLGQS
                     GLSLFVRKAGLYLLNGEVFLEIFPSQKVKETETRNKTAVAGREFVIHRIEKDQKQQYY
                     HVKSSVSGMYYDLLIPLDEEFVSLLQTLIQIPEPEKEVS"
     gene            complement(32771..33427)
                     /gene="deoC"
                     /locus_tag="PLP02_01505"
     CDS             complement(32771..33427)
                     /gene="deoC"
                     /locus_tag="PLP02_01505"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPF42178.1"
                     /translation="MKINKLIDHTYLKAFGTVKEIETLVAEAIKYDFKSVCVNPCHVK
                     YCKEQLLNTDVLVCTVIGFPLGANTTETKVFETLDAIRNGADEIDMVMNIGAFKEGND
                     EYVKQEIEDVVKASLGRTVKVIIETCYLTEEEVIRASELVGKTEAAFVKTSTGFGPTG
                     ATMENVKLMKDNCNGKEVKAAGGVRTKEDLEKMLEAGATRIGTSNGVALVKGETGSGY
                     "
     gene            complement(33580..36327)
                     /gene="ileS"
                     /locus_tag="PLP02_01510"
     CDS             complement(33580..36327)
                     /gene="ileS"
                     /locus_tag="PLP02_01510"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPF42179.1"
                     /translation="MDNNKNNYKDTLLMPNTDFPMRGNLGQREPEIQAKWDDLDLYRK
                     VMKKNEGRPLYVLHDGPPYANGSIHAGTAMNKILKDFVIRYRNMAGYKAPYLPGWDTH
                     GLPIENALSKDRKVDRKSVEVVAFRKLCAEYAKEQVDIQRRQFKRLGVLGEWENPYVT
                     MDKRFEAAQIRLFGQMVEKGLIYKGLKPVYWSPSSESALAEAEIEYHDVKSPSIYVSM
                     PVVDGKGILPDDALMLVWTTTPWTIPANLAICAGPDIEYALFTTDNKKKYVAAKNLVE
                     QLKGILGWEKTEILHEYIGSELEGITYKHPLYDRVSPVILGDHVTIEDGTGLVHTAPG
                     HGEDDFLVGQKYNLEAYCPVDSLGRMTKEAGKRFEGLFVDDCNPEVIKALDEAGSLLK
                     SVEIVHSYPHDWRTKKPVIFRATPQWFASIDILKQDILDAIKKIKWYPSWGDVRLSNM
                     IKDRGSWCISRQRAWGVPIPAFYTEKGNPILNKTIIEHVATIFEKEGSDAWFTMSAKE
                     LLPEGYVNEESPNGIFRKETDIMDVWFDSGSSHHGAMIERGFPYPADLYFEGSDQYRG
                     WFNSSLITGIATEGKSPYKTLVSHGFVLDGEGRKMSKSLGNTVDPNKICNTMGADIYR
                     LWVSSVDYQADVRLSDDLLKQVSESYRKIRNTLKFLLGNLFDYDETKNKVPYGQLEEI
                     DKYIILKTDGLMKEVLASYDDFRFDDVYRLVNNYVVFLSAFYLDFSKDVLYIEKADDL
                     RRRQIQTVIYHATDSLLRIMTPIIPHTASEAYDLIPHREKEDVYLTDISMPEEYPDAV
                     EIIDKYDRFMEMREIVLKALELARNNKVIGKSMAARVVLYPNEEYQNLIDNLQCNLQL
                     VFIVSKLEIKSEGKGSYDENGLSVDVFPCEGVVCDRCWMTVPDTDENGLCPRCHDVLL
                     K"
     misc_binding    complement(36366..36570)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(36619..36876)
                     /locus_tag="PLP02_01515"
     CDS             complement(36619..36876)
                     /locus_tag="PLP02_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPF42180.1"
                     /translation="MPEILYYILTILYSILQVYFVVMIVTIILSWTSVRNTRTYQVLD
                     RISSLYLGYFRNWLTIGMFDFTPMIGLIVYQIILGIIARVL"
     gene            complement(36881..37198)
                     /locus_tag="PLP02_01520"
     CDS             complement(36881..37198)
                     /locus_tag="PLP02_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPF42181.1"
                     /translation="MGMLGKKEKIVATDISFEDLKFYRVQDTSGIYEYAEMIMHRLPV
                     VLNFSDCLITEANEVLMFLTGVLYACDGEIVKIQDKIYLMAQKTDLKGPTLQKFIHDY
                     TKK"
     gene            complement(37201..37854)
                     /locus_tag="PLP02_01525"
     CDS             complement(37201..37854)
                     /locus_tag="PLP02_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPF42182.1"
                     /translation="MPDSIRENAIRLLEETKGKTIVAATKYVGLAEIRELYEAGINNV
                     GENRVNDFLAKKEALSDIKLVWHFIGSLQTNKVAKMINEIDCLHSLDRLSLAEEIQKR
                     RIEVLPCFIEVHISPEESKSGVRPKDLRDFCEKVAKYDKIKVIGLMGMAPLTDDTGKI
                     RNSFALLARLRDDLRSKKIPGVDCNYLSMGMSDDYRIAMSEGATHLRLGRILYRNGE"
     gene            complement(37865..38047)
                     /locus_tag="PLP02_01530"
     CDS             complement(37865..38047)
                     /locus_tag="PLP02_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42183.1"
                     /translation="MSDKKQKRNFWISFSLYMANVIAGVVLIVIYKANISHVITISIY
                     LVILSAFFSYYIRKKN"
     gene            complement(38040..38768)
                     /gene="surE"
                     /locus_tag="PLP02_01535"
     CDS             complement(38040..38768)
                     /gene="surE"
                     /locus_tag="PLP02_01535"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPF42184.1"
                     /translation="MKILLTNDDGYDAKGIRILYEKLRKYGDVTLVAPAKHMSGASVS
                     RIFWNEAIVTKYKDDIYSVEGTPADAVAFAIHGLKLNPDLVVSGINDGFNIGTDTIYS
                     GTVGACMEALRDGYKCIAFSTDFGYYSPVEAETEAAMDFILAHDLVSSDYILNVNFVS
                     KRFDKSNGIKITDVGYYPFEYTYEKTENGHYRTRRHHLVNEFVEGTDYHAVHNGYISI
                     TPLKFGFQTDKGLEELKRKVAQYE"
     gene            complement(38794..40230)
                     /gene="mgtE"
                     /locus_tag="PLP02_01540"
     CDS             complement(38794..40230)
                     /gene="mgtE"
                     /locus_tag="PLP02_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPF42185.1"
                     /translation="MDEKEEIRLEEKDYIGEIVTLINAETDFSGLQKQLEEYHPFDIS
                     QALLEIDSERRRNVFLHFTYPFGAAIVEHFDSEDAIVLLKQLPLETAVRIVDNMETDD
                     AVDLLQNLEPKDEDIDIISLLSPKKRAELNKLLVYEEDEIGSAMSGSFIRLFANMTVK
                     EAMRKVVSIAGDTDYISILYVLENNFLVGYIRLKSLIIARAEERISDIMETRFITARP
                     TDDKEEVSNLMQTYGESSMPITDIDMHLVGIVTHDDLMDIVSEAKSDDYAKLAGLTDG
                     DIDLGRDSFSRAVKQRLPWLSIVLFLSLLTSLIFSFFEEALNTSIVLAARLAVYLPLI
                     LDMSGNTGTQSLAVMIRYLNSPERDTSKKVMGKYLLREIGTGIIEGLVIGVLVFGLVI
                     VTNLVSRGKAFDAVSLSTAVVTAFSVFVALLASTLLGALIPLGMDKVKIDPAVASGPF
                     ITTVSDIITLSIYYSISLAILLPIYQAL"
     gene            complement(40243..41640)
                     /gene="mgtE"
                     /locus_tag="PLP02_01545"
     CDS             complement(40243..41640)
                     /gene="mgtE"
                     /locus_tag="PLP02_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPF42186.1"
                     /translation="MENEDRTINTKEIIDVLKSNAELLEKREKLDNYHDYDLAVAMLE
                     MTEEERSDFYRILDFPLLSAVFSELDPHDAKDVLDNAPVAVMTAVFKGMQTDDVVDII
                     DCIDDSDMRMAYLSVIDAKKRIEVKKMLEYDDSLVGSIMNNNYLEIKRTDTVKMAIAT
                     MVQKAPETEFINNIYVVDKGILVGVLSLKEIISAGNKPDTLIEDIMTVKIVSVTPWQK
                     TEDAIELMKNYDFFLLPVVTGDFKMLGIIAFDDMFEALNKESDEDYSKLAGLTDINID
                     EDRETVWTTVKKRMPWLAILLFINLVTSSIITGYEDLLSRVLTLAIFMPLILNLAGNT
                     GTQSLGIVIRLFATNQLDDKKAIRKHLIRELLIGIANGLAMGILIFGIVIGMRMLEGM
                     AFVDVLPLAIVISVSLSASLAVATLAGSVVPLVLNLFKIDPAVASGPFITTVNDIISL
                     LIYFGLATTLLARYF"
     gene            complement(41739..42911)
                     /gene="ftsZ"
                     /locus_tag="PLP02_01550"
     CDS             complement(41739..42911)
                     /gene="ftsZ"
                     /locus_tag="PLP02_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPF42187.1"
                     /translation="MFDLNDKFNQKPNIKVIGVGGGGGSAINRMIENDVQGVDFVAMN
                     TDAQVLKLSKADIRLQLGKTLTRGLGAGASPDIGRQAAIESEDEIRDLLADTDMVFIT
                     AGMGGGTGTGAAPVIARIAREMGCLTIGIVTKPFGFEGRKRMSVAIQGLEELKPYVDT
                     LIVIPNDKLLYIVEKNTPYLDAFREADNVLRQGVQGITEIIAVPGVVNVDFADVKTVM
                     KDKGTALMGIGIAEGENRAVEAARAAIRSPLLETSINGATDAIVNITSGFDASLYEIN
                     EIIEEIQKSSTTDINVIYGSAINSDLQDEIIVTVIATGFSEDPLFKDTLLEKAEEIKN
                     DDILPVEEIFQKQKPGKKEKQKKNRNVREPEPLPKTKADEEEDIQIPSWLKERFKK"
     gene            complement(43069..>43785)
                     /gene="tgt"
                     /locus_tag="PLP02_01555"
     CDS             complement(43069..>43785)
                     /gene="tgt"
                     /locus_tag="PLP02_01555"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPF42188.1"
                     /translation="FGADIIMSFDECPPFYADYEYMKASVERTVRWAKRGKDAHEKPD
                     TQALFGIVQGGHYKDLRKQSLDSLMEIGFPGYSIGGLSIGEKKDDMYEMLEYLKGIMP
                     HDKPRYLMGVGAPDDILKGVECGIDMFDCVLPTRIARHGSAMTSEGKVVIKNKTYEED
                     MGPLDPECDCKVCQSYTRSYIRHLFKTDEILGLRLVTYHNLYFLKKLMADIRKAIMED
                     RFGDFKDEFIKRYYPKTENV"
BASE COUNT        11119 a        10142 c         9199 g        13325 t
ORIGIN      
        1 tttcatagtc agcacaattg tacatttttt tggcctgtac ttcaacaaaa tcgccaaaaa
       61 tcgttttatt ttgtatcttt ccatccattg atccaacctt tgtataatta tcatggaagg
      121 atctttatac aaaatggcaa acagatttaa aaaaagcgtt tctgtgggtg gcaaggcgga
      181 taatttgcaa tttgatatat tttctaaata aaattgttcc aaatagaccg ccaattcttt
      241 tttctccatg tgtatactgt ttgctttaag gattgtttta atcgctaagt tcacagtttg
      301 ggatccgttc attggctctg ttatttgatt tgtatccaaa agaaacgcat tttctctgat
      361 ttttcctcgg atacgaaaat ctgttctctt cgtgtctttc gataatatgt ctttagcaag
      421 ttcgcctagt tctagatcgt tttctccgat aatacccaat atttcgctct gtttgatgaa
      481 aacaacatct ttttggaaat tatcttcatt tgtttttaca tattttgcgt tttcttcatg
      541 caatgatttt aatatttccg ctgaaataag cggtccggct attgcttcca gccacagcga
      601 tgtatcttcg gctaacttca tgaaatcatt gttatccggg tgttccggca ataatctgat
      661 ggcttcattg taatggaaat caatttcctt taaaaggaaa tatgttttat ttgtcttatc
      721 gtaatcgtaa tgtttaatcg ttttggtaaa tctttttgca aaagcgtcaa aaaaagattt
      781 tgaacattgt tttgtgattt ctttttggat ttctggagta tccagaaata tattgcatag
      841 gagtcccaaa taatgcggat ttaaacaata tattcgatat aaattttcaa aaagaatgtt
      901 aatagcgttt ttaattgaca ccggcttatc caagataaag tcatcttccg atgaaacaat
      961 ccgcatattc gtgtataaat cgtcgattag agcgaaatat agatatttct tttccggaaa
     1021 tcgatagtaa aaactgcctt tgttgaaaga cgagcgtttg attatctcgt ttagcgatgc
     1081 cgattcataa cttttttctg caaactcacg taacgtttct cttaacaatg attcgttcga
     1141 tttcaaaaaa tatgtcaaat ctccatcttt agtcgacatt aagtcctccg tcgttatacc
     1201 acatggttta gaccgcacgg tataataata tcatcctgtt tgaaatatgt caacgactta
     1261 tgttataaaa agcctatgat taggttattt tcgatatttt tttcctaatt tttcttttag
     1321 aaaatctttc aaaatattta aatcattgtc tgcttcaaag ccgtttttat cgacaataaa
     1381 ggttttcttt tcgagatcgt atacaatcaa gaattctttc gattccttta tccggatgat
     1441 attttcatac ttgattgttc ttgcctttat gttgaatatc tccacttcgt cttctttgaa
     1501 aacgtatgtc tgatacaccg gattagccac tatcggactt cttttcacag ctctcttggt
     1561 cgtcagcatc tgcatgacaa tcgccaagag gaaataggct gccaatatac ctaccatcgc
     1621 caaagcgtat tcccattcgt tttgtacgag cagatatgcc ccgaaaccgc aagcggtcag
     1681 aatgacgaac aacgagtttt ttttcaagaa atccataagg tagtaatgat tgtatctgat
     1741 aatcaggtcc ttgtcataaa gggtcttatt ccttatttcc atattctgtt caactcccga
     1801 tttcatattt tcactttttt gaacaaaagc ggatatactc cgattcctat aaataccatc
     1861 gccaaatacc ttatcatgtc aagaatcagc gcgattgttg ccttaaacgt gtccccccag
     1921 gcaaacgccg atgaatcgac aataaatccg aaaagatact tcaagacata acgaacaccc
     1981 attacaatgg caacacctag agcaaaacga atgatgtttt tccaaattac ccggtggttt
     2041 tcaaagttca cacgcttttt ttcgaatgag atacccaaaa cgaatcccag catcgcacct
     2101 gtcatcttga aaagaccgac aaggccgtaa taaacgtaat ccgcatccgt cgtttgcata
     2161 gcgacgtcaa acgacggcat agaaataatg taaaaaagat atgaagcaac caccagcacg
     2221 atgctcgtta tggctgtaac ccagtagatt aaggcgcgat ttttaacttt ttgaaaaaga
     2281 tagtagaacc cgatagcaaa tgccagtcct aacaatcctc cggcgatgac atccgtcaga
     2341 taatgggctc ccagatacat tctgctaagc ataaccataa tgacaaccac gatgcttagt
     2401 atggtcagcc aatgcttttt catccacgta gacaaactga agaatgtcgt agccgcacct
     2461 tgtgtgtgtc cgcttggaaa cgagtatttg tcagtggtgc tggatctgac attaacaata
     2521 tccgcatcgg catcataagg cctgattctc tgaacaaatc ctttcaaagc gctattgaag
     2581 atcaatgaag cgaaaacggt gattccgatc gcttctcctc ttttcttatc caggcaccag
     2641 aacatgaatc caaggatacc tacgatcact agttcttctc caaacattgt ccaaagctgg
     2701 aagaaaagat cccagaaacc attacgaaaa ctttgaaacc atttgattat ttccacttcg
     2761 aaaggcatta tatctcctct tttccttcta atatggcttc gagtatccgg ctgacgccgt
     2821 cttcttcatt ggaaggggcg atcagatccg cttgtttttt tacaatatcg gtcgcattgg
     2881 ccatggcaac gcctaatccg gcttcattga gaagcgatat gtcattttct tcgtctccaa
     2941 atgcgataat ttggtcttga tcgatcccga gactatctgc aagatctttc atcgcgcttc
     3001 cttttgtggc gattttgttt gagaattcca atagaaacgg ggcgcttttg aatactccgt
     3061 atttttcctc tatttcgctc ggaagtccgt ttcggagtat gtcgagtttt tctttgctgt
     3121 cgaataccaa aatcttatat gcccaggcgg gaatattttc cagattcttg cttatcgtcg
     3181 taaaaccgtc catggcttct tttccgagaa tctgagcttc tgtcaatccg tcggaaacca
     3241 tcaggccggt ttcagggtat agatggcaaa acataccggc atcgtgaatc gcaccaatga
     3301 tacatgaaag gtcgttatgg gaaagcgtcg ttcttttgat tatatccata ttgtcagcct
     3361 tgatagtcaa agcgccgtta tacggaagca ggtatgcctt gtcgtacatg tctaactgga
     3421 aatccgatac caaactcatg atttcttctt tcgatcttcc ggaagcaatc actatgaata
     3481 ttccggcctt tttaagctta ttaatcgttt caacggttct ttccgacact tttgatttcc
     3541 tgccaagcag ggttccgtca agatcggccg caaacattct tatcatcatt ttacctcaca
     3601 tcaagccaaa gacaagcaag cctaaggcac cggatatcag aataagataa atcggattca
     3661 tttttttgaa tacgatgaca aatgccatca gcacaccggc tatggcaaga agacgccagt
     3721 ccaaaatcgc ttggcttata tccgtaattt cggtcgagaa aacatttcgg accaaaaagg
     3781 cgatgccaac cgacaaaata atgccgacaa tagccggttt gacccctgaa agaaatccct
     3841 gaacgtacct gtttgtggct atcttcgtaa agaattttgc cacaagataa atgattatga
     3901 aagaaggcat taccacaccc aatgtggcga aaatcgcccc gaaaactcct gcttcatgat
     3961 aaccgatgaa ggtagcgatg ttgatggcaa acggtcccgg tgtcgattcg gacactgcga
     4021 tgaagtccac aagtgtatcc atggaatcaa gccactgatt gtttatcact tcgtcttcga
     4081 tcagcggaat catggcataa ccaccgccga aggtaaacag accgactttg aaaaaggaca
     4141 ggaacaatct gataagttca atcattcttg ctcacctgtc ttttcatgat gataaaggtg
     4201 cgtccgagtc cgatgacgcc agaagccgca atcaggatca agaccggaac cagatcgaaa
     4261 agagcaatga aaaaaccgac ggcgatcatt atatagccga aaatatcttt tttaagtttt
     4321 ttgaacaatc gaagtccggc catgatgatg agaatggaaa ctccgacacg gatgcctttg
     4381 aaagcatatt ggatataaat attttccttg tactgcaaga aaaacagcga tatcagaaga
     4441 atgatcagaa acgacggcat aataacgccc aaagtcgcaa aaagcgcccc taggaagcct
     4501 tttttgcgat atccgatata agttgccgaa ttaacggcga tcggccctgg agtggattct
     4561 gcgatggcga tgatgtccaa gaattcatca ttgcttatcc atttcttttt ttccacaatt
     4621 tccgcttcga tcaacggaat catagcgtag ccgccgccga aggtgaagag gcctattttg
     4681 aagaaaagcg cgaaaagttc aagacatttt cccgctttat tatctttttc aggattttcc
     4741 ggcattgttg tagatttcca gcctttcatc gatcagggca tatatcctgt cgctgacttc
     4801 ggctgtcgac ttatccttga tctcttcata cggaacaaaa ggcaaaatat gcagccggca
     4861 agtatggatg ccaaaacgcc atattttgat tattccgtgc atgtcatata aacagacggg
     4921 ggaaattccc gcgtttgcct tcaaggcaat tttgaaggct ccatggcgga attctcccat
     4981 tttgttttcg tagcttctgg tcccttcggg gaatatggcc atcggttgtc cgtccttgac
     5041 ggccttgatc gtatcgagaa tagcctgcaa accttgtttg gtcgattccc ggtctaaaga
     5101 aatgcatccg gttccttcca tcaaggtttt gataagggga atccggaaca attccttctt
     5161 ggccacaaac gccagattat acttcgagta tgcgaatttt atatatacag gatccgaata
     5221 ttccatatgg ttagatacga ctatgaaatt gccatcttcc ggtttcggaa gattttcctt
     5281 gccggtgatg acgatcctca ctcgcaaaag gacgccgaac acatatggaa tgaaaaaggc
     5341 gtaataacga tgtttggcaa tgcttttctt gtcggttccc ttgagaatgt aaatggacgc
     5401 gatgatccat aagattgtga aaaccgtcaa aatgaacaga atgatgagaa aacaggcgag
     5461 catgatcaat accgcccagc cattgtgcgg ataatttccg tataatccat aaaatgcggt
     5521 cccgatggcc gccagaacga tctgaatcag aaacactatc atttttcttg cttcctctcg
     5581 tatatggcga aaacgaggtc gcccgacttg ttagactgga tcagatcgaa caaggataga
     5641 tccaatcctt cgaagtagac gtttccttcg tattctttat ttatatgggt gatatatagt
     5701 ttctgtgcat atggcaacga ttgttcaaaa accgacttcc cgccgattac gaatatctct
     5761 tcatcgtgtt ctcctttgag atacgtgacc aaatcatcta gaattacgat gtcgggatag
     5821 ctgaacggtg tttttgatac aacaaccgat ttccttccgg gtaatggttt cccaaggctg
     5881 gacaggattg aaagaaaggt gttttgtccc attagcacgg tatgcccaag agtagtcttc
     5941 ttgaaataag ccaaatcctc cgggtaatgc caggggagat tgttattgga gccgatcaac
     6001 cggttcttat cgatcgcaaa gatcatactg atcatttaga ccgccacctt tcccttgacc
     6061 gccggatagg ggtcatatcc tgacagttca aaatcatcgt agcaaaagcc atcgatcgat
     6121 tcgacttttc ttttgattcg catttccggt aatttccgcg gctttcttga taaaagcacg
     6181 tcgacttgct cacgatggtt gtcatagata tggcaatccc ccaacgaatg gacaaatgtg
     6241 cccggttcgt atccggtcac atgtgccatc atgatcgtca gaagagcata tgatgcgata
     6301 ttgaatggaa cgccgagaaa cgcgtcagcg cttctttggt acaactggca gtcaagttta
     6361 ttgccggtaa cattgaactg aaagagcgta tggcagggag gaagagccat tttctctatc
     6421 tccgcaggat tccaggcaga aattatgatc cgccggcttc cctgattgtc cttcaacgat
     6481 ttcaccgctt ccaacaattg gtcaaccgtt ttatccttgc cttcaaaatt tcgccactgc
     6541 tttccgtata cagggccgag atctccgtgt tttaatgcaa aaacaggatc atcggcgatt
     6601 tttccgataa attcctccat ggtttcgtcc cgataatcgg gtgatttccg aaaggcctcg
     6661 tatggccact cgttccaaat cctgactccg ttttggacca aataacggat atttgtatcg
     6721 ccggatacaa accatagcag ttcatgaatg atagatttca catgaatctt cttcgtggtg
     6781 agaagcggga acccttccga taagtcaaaa cgcatctgat aaccgaaaaa ggatgtcgtt
     6841 ccgacacctg tgcgatcatt tttatgtata ccttcggaaa ggatcttttc aagaaacttc
     6901 aggtactgtt ccatgcccat cacccatatt tgcaattata ccattgtttt gtgggtaaaa
     6961 aaagaaaaag atgcattttg catcttagtc tttctgagaa cttatgaacg atattttctc
     7021 gacaatgatt tccggatacg agaacatttt tttcccatcc tgcaagtaat cacgctgttg
     7081 taagcgggct ttgactccga cgattgaccc tttcttacaa taatccacag tggcttccgc
     7141 gatcccgctc caaagcgtgc aattgaagaa atctgtctcg tattcgtttg tttccccgct
     7201 tttgaacggc ctttgaacgg caatcgatat cgtcgtcacg ctgcgattgt cctctagttt
     7261 tcttaactcc ggatcttttg tgagtcttcc gacaagtatc acctggttta gcatatacat
     7321 tcctcctttt tcattcataa taaacgaaag acccgaaaaa cgacaacttg cattttccgc
     7381 tttggctttt gtaatatgac aaaaaagcga tgtatttttt acaaatacaa aaaagcggct
     7441 tttatacggc cgcttttgta tttttcttta ttaatttacc atgaaatctg tatttattaa
     7501 aacttgaatt cgacgtcctg tcttttcttt tcttccgctt cgaaataagg ctttttgtcc
     7561 atgttgatag ctttggccac gatactttgc gggaatgtca cgatgatgtg attgatgctg
     7621 gtgacattgg cattgtaaag tcttctggcg gcttgcagat gctcttcggt gttcgccacg
     7681 gcggactgga gttcaaggaa aacagtgttt gctttgagat ccggatattt ttccacgact
     7741 acgtcaattc cggatgcgac tttatttagt tgtcccaaaa attcttcttt atccttgaga
     7801 gttgcgcttt cagggatgcc tgaacgccat ttgacaacct tttcaagcgt ttcagcctca
     7861 tgtttggcat aaccttttgt ggtttccaac atttttgtga gcatgtcaaa ccgtttggta
     7921 agggcgacat cgatgccgga ttcgccctcg ttgactttca gttccaactg acgaagtttg
     7981 ttcatgacgc cgatatacca gaatacaatg atgagaacga cggctactaa gacgatcagg
     8041 gctacttgcc agccttgaag ggaaagggtg accggtgtat acattttgtt tcctccttat
     8101 tcttctttaa tatttatgtc gaaacatatt atatcatatt tctttaaaaa tctttttatt
     8161 caatttcaac aaattaataa aatcgcgtac agcaagaatg tcctgtttga aagaatccat
     8221 cattgcctgg tcaattttac ggaacatttt tatttcaaac gtatcgcgat tgttgttgat
     8281 ggctatgtaa agatcttttt ccgagaatga aaagccgatt ctgccaggat tgcgtttttc
     8341 cagttccatg atggcttcca ttatatcggg agtcagaaca taaaaagcgg aaatctcttc
     8401 ggttgcgtat gtcttgaatt gcttgttgaa atcgattgat tccagtttta ctttcttaaa
     8461 aggccggttt gaaagcggcg atccggcttc caaaacttga agatagccat caaacggctt
     8521 gttgaaagta aaacggaata ctcttccgac gaaatacggc acgtaatata ctcttgtacc
     8581 gttttgggtg tgctggacat gcctttcttc caaacgcacg tcacttgaga taaatccgac
     8641 atcatcgatg ttgcctgtta acaaatcctc ggtataaaat cgatcagccc gtttcaagaa
     8701 ctctgtcgca tagacttcca tttgcgacaa accgcgctcc gggtaatatt cggtgcctgg
     8761 caaggcctcc gaaaaatatg ctttcaatac ttctttctta aatcgccttt tgagcatatt
     8821 aaattgagtc agcccgacag ccccgacaat gaatcctgct agcccgacga tgaaagcgat
     8881 cgccgccata tcttcggatg ctgtcgcact gtatattgcc atgtaaatta taaatgccaa
     8941 aaccaccagc attatgccga taagtgtcag ggtttcgctt ttcttctttg tttggttgaa
     9001 cacgtcaagt ttgctcttca ttttatctat cccgcctatt tccaagccat acaggctttg
     9061 acggctttct tccatccgtc atataattct ttgcgtttga tttcggtgat ggaaggtttg
     9121 aacactttat cgacgcccca catcgccctt atcttttcta ctgtccagaa tccgattgcc
     9181 agtccagcca gatatgcgac tcccaatgct gttgtctcgc tggtttccgg tttgtctatg
     9241 tagacgttga gaatatcgct ttggaattgc attagaaaat cattttggct ggcaccgcca
     9301 tcgacccgaa gccggttgag ttttattttc gattcgcttt ccatcacttc gagaacgtct
     9361 tttgtctgat aagcgatggc ttccagggtg gcgcggatta tttcttcccg gcctgttccc
     9421 ctggtaattc caaaaatagc tccccttgca tccttatccc agtaaggggt tccgagtccg
     9481 acaaaggccg gcacgaaata gataccgccg ctgtctttaa tgtttttggc atatgtttcc
     9541 gattcaggag cgtttttgat gaccttgata ccgtctctta accattgaac cgcagatccg
     9601 gcgacaaaga tggatccttc gagcgcatag gtcggcttgc catctatata ccaagcgatt
     9661 gtcgtcaata gtcccttttc cgatcttacc ggttcattgc cggtattcat tagcaaaaag
     9721 catccggtac cgtaagtgtt ctttacatcc cctttgttaa aacatgtctg tccgaaaagg
     9781 gctgactgtt gatcaccggc aattgccatg attggaattt catgtccgta gaaaacattc
     9841 ttggcggttt tcccgaacag tccggaagaa ggttttactt ccggcaacat cgacattggc
     9901 acttggaaca aatcgcaaag ttccttgtcc caagccaaat cgaaaatgtt gaatagcaaa
     9961 gtacgcgagg catttgtata atcggtcacg tgcgcgtggc cgccggacaa attccagact
    10021 acccatgaat cgatggtccc aaatagcagt ttaccttttt cggcagcttt cctagcgcct
    10081 tcgacattgt caagaatcca ttttatcttg gtggctgaaa aatacggatc gataagaaga
    10141 cccgtttttt cccttactat cggttcggct cctcttttgc gcattttctc acaaattccg
    10201 gttgtctgtt tgctttgcca cacaatggcg tcgcaaacgg gttttccggt ttctttgtcc
    10261 caaacgactg ttgtctctcg ctgattggtg attccgatcg cggcgatttg cgccggattg
    10321 atcttcgcct tgaccagaca ttcaccgata cagaccgaaa ccgattccca tatttccatc
    10381 ggatcatgct gtacataacc tggttgggga aacttctgac tgaattcttt ttggcttagg
    10441 caaaccatct tgccttcgct gctgaagatg atggcccggg aagatgtcgt cccttggtcg
    10501 atcgcaagga tgtatttttg cattttcaca cctctttcct aatcattata acaaatcgta
    10561 tgtcataatt aaaccgcaaa tgttataatg tctttatatt gtaaagaaaa attatattat
    10621 tttaaggagt gatttttacg aaaaaaatca tcagcaagga ttcacagggt aatcccatcc
    10681 atatttacct ttacgaacct caggaaaaac ctgttggcgt tgtcatgatc attcacggcg
    10741 caagcgaaca ttttgccaga tacggccttt tctccgaata tctgaacaaa agcggatttg
    10801 tagtcgccgg ttgtgacatc ctcggtcacg gtctcagcac cgacaccact gcctacgtcc
    10861 atttttctga caaaaacggc gacagtcagg cttttgaatc cgtattgctt gtcaaggatc
    10921 tcatcaccga aaagtatcct gatctaccaa aatatgtctt tggtcacagc atgggttcgt
    10981 tcctggcgag aaagctttgc atcgactatc cggattattt ccagaaagct gtctggaccg
    11041 gaacgggatt tgtttccaaa tctactatca tcatgggtaa agtcatcgga aaattactta
    11101 tcatgtttca cggaccgcgc tatgtctcta agttcatcaa cggaattgcc attgacgctt
    11161 atccgaagaa aatgcgtgaa cgcggaatca tcagcggtat tgatgaagag tggcttacga
    11221 aagacaaagc catccaagat tactatcata actcaccaat ctgcggacag ccattcacgc
    11281 tgggtgccaa cctggctatg gcaaaatggg ccgactatgt gaacaaaccg gttaacatcg
    11341 ccaaaatgaa tcatgatcta ccgatcttta tcggttccgg acaaaactgc ccgctcggag
    11401 attttggcaa gggcgtcacg agattatatg aattaatgaa gaaacaaggc ttcaaaaatg
    11461 tcgaactcaa tatttatccc gacgaccgcc atgagatcct gaacgaactc gatagggaca
    11521 aagtatataa ggacatcatc gattttttaa aaaaataaca atattgcatg caattaccaa
    11581 taaaacggat tctccttccg tatatcttgt gtaaaaacct aaaaggagga ttcaaacaat
    11641 gaaaaaagta atgttagcca ctacaatggc tttcgcccta atagccggat tattcctagt
    11701 aggatgcgat caagccaccg caacagacga attgtcatac gccaccatcg agatcaatcc
    11761 cgcagtggat ctggttctga tgaataacac cgttcagacc gccacggcct tgaactcaga
    11821 cggagaaatg ctcctgaccc agttaagtct caacggactt tccgctgaag acgccgtcaa
    11881 cgacatcatc gacgaagcca tcgatctcgg atacattgat cccgaaaccg aaggtgtcac
    11941 tgtccaggtg tcctgcacca atgaaggtac acagacgatg ctgcaagaaa aaatcaatac
    12001 cgctttccaa gaacgtggaa tgttcggtcg cgccgtagcc gcccagaatg ctgactacat
    12061 cgcagaagcc gaagaactcg gagtcagcgc cggtttcctg agacttgtat accgtgccat
    12121 ggaagccgat gacacccttc tgaaggaaga tgcactcgtt atgtccaccc aggatttgat
    12181 cgccattgtc aaagacaaca acggcaatgt caacggactt tcccaggaac tcaaggatga
    12241 attctttgct gcacgcgatg acctgagaga cgaatatctt ccacaaattc aagatcttca
    12301 agcccagatt actgctgctg aagccgacgg cttagacact accgatcttc aggctcaact
    12361 cgatgctctc gtagctgaat tccatgatga actcgcctta ctgcgtgccg attaccaact
    12421 tgaaggcgct gcccttaaag ctcaggttca aagcgaaatc caggcccgta tccaagccca
    12481 tcaatccgat gtgcaggcct atcgtgctca gattcgcgaa agaatcagaa cttacaaaga
    12541 tgaaatcaaa gcttatcaaa acggaagcac aacccaaacg actaccacga ctactgctac
    12601 ttcgggtgag gaaaacacca accagaattc aacttctgac ggtggaagca actgagacat
    12661 caatatgaat caaaaaagtc gatcaatgat cggctttttt tattttatat gtttaattat
    12721 ttctctgaaa tctatacgca gtcccgagca ttcgaatata ccatcttcgc tcataaagaa
    12781 tttcccggag agacaattat tggcataata tcgaatggtt ttcaaataag tgaaatcctt
    12841 tggctcaaaa cacagaggaa aaaggtgctc gaagtctgcc agataccaca ttatcatacc
    12901 ccaaactaca acatttgtat aaattttcat gtctaggttc tcaaaacaag gagcacaggt
    12961 aaaacataga tcaatactgt ccattatcca tatttcatca actgaattca taaataaaga
    13021 gattactgcg ataatgacgt ttccggattc gttcttcatc aatatgagta tgattttctt
    13081 tttgctatca atctccgtca tatcgaagaa aattattttt gcttttttcg catttgattc
    13141 agactttttt gtcaaagaac tctcaaacgc ttttgttggg atgcagtaat tatccagtac
    13201 ggcgcaaata caggtgtgca attctctata atcagaagaa tttgtttttg tcatgggttg
    13261 tctcggaaaa tatcttcaat tcctttgggt tgttcaaacg cattttccaa ccgcctgttt
    13321 tccagtcatt tccttctctt ttgacaaatt cgtattcaaa tttcccggtg taaaaatctg
    13381 tttccatatt caatctgtta aatttggtcg ttgacatcct gttcggttcg tagcgcattg
    13441 atttgacttt tgcaataccg ttttgaatgt ccacgctcac tattttccaa atgtgctcca
    13501 tagtggcttc cttgaaacgc ttgtatttca tgaactttat cagattggcc aacatgtctt
    13561 ctttggtttc cgtttgattc ttaccattcg ataaaatgga attcccgaaa caaaattccg
    13621 agtcgatgca ggatttgaat atttcaaaat cagcatgatc gattccccaa ctgtatcgat
    13681 aataagcatc caaaacctgt tcctcgtcgg ttcccaagga ttcaggattt tctatcaccc
    13741 gccacggagc gtggatttca cttacgatcg gataagaaat atttccttcg aaatagcgat
    13801 agtcgataag cttccaccag tttctgacaa aacacgtgtt cccgtcttca aaatccagat
    13861 tgaatctgat gtgcgaaatt ttccattttc cgttttcctt gacgtaatca ttaagatagt
    13921 atccgccgaa gttaaaataa tggaagtaat cgtcgtcgtt gattccaaca agtccagtca
    13981 caacagcgct ttgacatgca tggccatttt ccgtaaaaca aacgaaatta aaaatcttat
    14041 aacgggaata attgattggt tcgcctgacc agctgatttt gttggcgacc tgttttttcc
    14101 cgtttgcgtt gccataattc gtggattcca aaataacgtt ttcggaaaac aagctttcca
    14161 tcaatgcaac atcgttcgat tctaaagcat cgacatatgt agaaaatact ttttttactt
    14221 ccaatctctc ttccaacttc attcgcctct ttcaatttct aataccggtt cattgtcgaa
    14281 agcatctata ttcttttgaa ttttatatcc ttcattcaag ttcgtcacta caacaatgac
    14341 cggaatttcg tagcctttct ctgctatttt tgcgaattcc accatgatga gcggcatccc
    14401 gacgacgacc ttttgtttca tccgggctaa ggaatggaaa aaggatccgt tcagattcac
    14461 tgtatctatt ccgatatgaa tcaaaatttc cgtcccattt tctgccgtga tagcaatggc
    14521 gtgatttgtg ggaaacacgg ctgttatttt cccgttaacc ggtgagacta tttggttact
    14581 tactggattg acggcaaatc cgtcgcccaa catatgcttg ctgaaagctt cgtcgcttat
    14641 ttcttcgatt gggaatacat gccctttcac cgggctatat acaaagcatt tttcatgttc
    14701 tttcgttttc ttttcggcat gcaagaaatt caacataagc ttgttccttc ctgtttattc
    14761 ataatcttga tcaaatgtag cataatataa aattcttcgt ctttatttac caatatgttc
    14821 aattcttttt gaatataatc ctttatactt tccacacagg catactgaat cggatattta
    14881 tgccccaaat ccgccaaaat ggtttccaca ccgtcgattt ttgatttttc gctttgatta
    14941 gcgaacgccc tttcgataaa atatcttaga tgtgttttga atctttcgta ttctattgat
    15001 tcggtatcga ttttgttttt ccaaaaccgt ccgacaatca aaagtacttt gtttgttatg
    15061 tctataacgc gattcatcac tccgatatcg ccgctgaatt gagcatcaat cagatgcata
    15121 acgatgaatg tgtcttcatt ttcatcggtt atcacattga atctttcatt catgcaagcc
    15181 acgattttct ttgccaaaac aaattccggt tcatagaatt ttcttatgtc ataagtaaag
    15241 aaattgggaa tatgaatccc ttctttggct cttttcacgg ctgtgtagat atgatctaaa
    15301 agcgaaatgt aaaacccgga cgaaatgaca aaatgggttt cgtcaaaaac caatttttcg
    15361 acatcgaaag tgaaatccca gtattcgaac ggtatggctt cgataagttg atagattcta
    15421 gcggaaatat tcgtgtcgtt agatagttca aaggtctttt cgattttctc ttcttcgatt
    15481 ttatcgccga tttttttccc gaaagccaac ccgttgccca tcactaccac atctctgccg
    15541 tttcgttggg attcagcgac attattattc aatattctgg ttattttcat gataaacctt
    15601 ccgatcgata tatattggtc ttgaaagcca atgatagtgt cccgtttgga ttgaaataat
    15661 catgtcatac aatatatatt atattacgtc ttgacgacaa tatgtccata agagccgata
    15721 ttgtgtcatt ttgtgtttcc gttcgtatct atacttatat actgccgatt caatttaaag
    15781 attcgccgtc tgtctcaatg acttttttat accaattgta tgagtctttt tcgcttcttt
    15841 caaaattacc gttgccttcg tcgtccctgt ttacataaat caggccgtat cttttcctgt
    15901 attctccagt gccggcgcta actatatcga tgattcccca gatggcatat ccaaaaacat
    15961 caacgccgtc cttttccgct tcctttatct gctttatatg atctctgata taatcgattc
    16021 ggtattgatc gtgaatgctt ccgtcgtttt ctaccttgtc gatcgctccc aatccgtttt
    16081 cgcttacaaa aacgggtttg ttgtatctgt cgtgcagttt gttgagcaca attctcaatc
    16141 cgatcggatc agtctggtat ccccattccg tggttttgag ataattgtta tgcataccgc
    16201 cgaaattaga agcgttacgc attttttcct gattggccga aacgacattg ctgaaataat
    16261 aactgaatgt gatgaagtcc acagttcctt tttttaggat tttctcgtcg ttttctccaa
    16321 aatgtatttc aacgtttttt tctttgaaaa accgtttggc gtatgccgga tatgctcctc
    16381 ttgcctgaat atcacccgcg atatagttat acacctcttc tagttgcatc gcaaacagtt
    16441 gatcagatgg atcggtggtt tccggataat ataggccgta ggcgatcatg tttccaaaat
    16501 gaaatttttt atttatgctt cttccaacta tcactgtttt agctgcggca actatctggt
    16561 tgtgaaacgc ctgggctttt ctttgcctta acgattcatt cggaatgtcc gacatatttt
    16621 cgtaatacaa tccctgcaaa agaaggctgt cgccatattg ttcgttcgag agtatggatg
    16681 gcatatcact tactacatcc atcatgctgg tattgatttc attgaatgtg agccagtatt
    16741 tcacttgatt cttatacctt tcgaaaagcg tggtggcata tttgacaaac aaatctatca
    16801 ttattctgtc tgcccatccg tgatattttt tgcataatgc caagggcata tcgaaatggt
    16861 tgattgtcac taatggttca atattgtatt ttttcagctc tttgaagaca ttctcataaa
    16921 acttcaaacc attttcattg ggatattctt cttctccgtt cggatatatt cttgaccatg
    16981 ctatggacat tctaaacact tttatcccca gttttccgag taaagcgatg tcctctttgt
    17041 aatggtgata gaaatccacg gcaacatgcg acggatatgt tttgtctttt tgtaagaccg
    17101 gagtgggata gcgtttttct tttcggtttc ctccagtgat aatatccgaa gtactaagac
    17161 cttttccgtc ttccaaatat gccccttcga attggttggc ggcggtggcg ccgccccata
    17221 gaaaatcttt tctcatcgtt ttcttccttc ctcggcggtt caaaggggca gttgtttccg
    17281 cctgcccctc tgatcgtctt tgcaattatg attatgcgtt ttcagtttcg ggattcttag
    17341 ccagatcggc tggatccttg tagaaaataa aggcaaggac acctgataga attgtggcta
    17401 atataactgt cgatattacg gcgaaccatg tggaaccgtc accgatgaat agcggcatga
    17461 acaaccagct ggctccagca atgacatagc acttgactcc catcaaaccg gcatatagtc
    17521 cggtgatacc tgcggaaata caagttgtga tcaaagcttt tttggatctt agcaaaactc
    17581 cgaaaattgc cggttcgctg acgtttccta tcagcatggt gactccgctg gaataagcgg
    17641 tgcttctcat gacgcggttc ttggtcttta ttccgaccac gattgctgtc gtaccgattg
    17701 agatatgggc gcaaagcgcg gcaaccatga ataccggatc atatccgacc gtgacatacc
    17761 atgtcaggat aataaatgtc acaatcgctc cgtgtaatcc gaacatgatg agaattggca
    17821 tcaatgccgc caaaatcatg accaaaatcc acgtgtaatc agagattgct tccaaaggtt
    17881 tagcaataag aattgccacc cagtttgtca acggacccgt caaaaccaac gccaccggag
    17941 tcatgatcaa tatgatcaat actggcacca acaacatttg agcaccagaa ggaatgtatt
    18001 tctttaaggc tttttccaac aggctcatga catagacagt aagcaatatc ggcaagaatg
    18061 attgtccgta tgttgtcaat gtgatcggca gtccgaatat cgtcagcgga gttccggcat
    18121 ttacataact cacaaatgtc gggctcagca ttatgcatgc cattaagacg gccaaaattg
    18181 gatcgcttcc cagtttgcgc gcggctccga tcgctagaaa tacaggtatg aaataccata
    18241 tcgcatctgc cgcaaaataa agaacgatgt aagtgttgtt atcggtcccg agccatccga
    18301 attgaatcaa catcgttaga atggctttga tcataccggc tccgatcagc ggaatgatag
    18361 cgacaccgat cgatgcgctc aaaaactcgg caatcgaagc caaaacatgt ttgaatcctt
    18421 tctttttgtc acttttttcc ttcttttcgc cttttccttt tgacggcatg aaaggaaaaa
    18481 catcctttgc atgagggccg atgatcacct gatactgtcc cccgaacgac atggtgttga
    18541 tgacaccatc gatttttttc agcgcttctt cgttgaattt ttccgtgtcc ttcagtacga
    18601 agcgcagtct tgtggcgcag tgggcaacgt ttacgatatt gtcctctccg ccgacggcaa
    18661 gtacgatcgc gcttgccaac tctttgtaat ccattgcttc ctccttatat ttttgttgct
    18721 ttatcctttt taataaaaaa acccgaatgc aagctaaaag cttacatccg ggttatgcct
    18781 gaaatgcagt aacattccct ttttcgtctt gattttatca tcagattatg cacttgtcaa
    18841 tatattatgt gaaaactctt tcatcacaaa aagccgaatt tctaattatt tcttaaaaac
    18901 attgtcagtt cccttattgc cttttcttcg tccgaaccga tcgcggtcaa tttaacggat
    18961 tcgtttttcc ttactcctaa agaaagaacg gaaataacgg atttcgcgtt cacgccaagg
    19021 tcttgtgatt ctgatttggc tattgttact tccgattcga actgggcagc cgcaaaagca
    19081 aaactggctg cgggtctagc atgaagccca ttttcacatt caaccattac atccttgcta
    19141 accatttcat tcggctccct ttatagtttt ttgttaatgt aatccaatac ttcatcttgt
    19201 tttccgaaat tcaaaacatt ttctgcaatt tcttccagtt cgacaagcga ttttgtcgtc
    19261 acaagttcct ttatgtttgc caaaagactt ggacttacgc ttagttttct gattcccatt
    19321 ccgataagaa tcgccgcacc gcgagaatct cccccaagtt cgccacagac tgataacggc
    19381 tttttatttt tgtgaaaagc atcaatggcg attttaatga aacggaacag acctggtgtg
    19441 aaactctgat aatatggtct gaccagatcg ctcatgcggt cggttgctgt taagtattgc
    19501 gccagatcat ttgttccgat actggcaaaa tcgacttcct tactgatttc ttctgccagc
    19561 atgacgatcg agggaatctc gatcatgatt ccttgtttga ttaaaccgaa tggaatattt
    19621 ttttccagta actctttttt tactttctca acaaatccct gtattctgtt ccagtcgtct
    19681 agacttccca ccatgggata cattatctgt agatttccat acgccgaagc acgaaatgca
    19741 gcgcgcagtt gcgtgtgcat tatctcagga ttttcaaaac atagtctgat ggcacggcat
    19801 cctaaaaatg gattttcctc ttcgggaagc ggcagataat tcagtttctt atcgccaccg
    19861 atatctaatg tccgcaaaac gaccggtttt cctgcgaagt cctgaagaac cgaacgatag
    19921 acgtcgaatt gttcttcttc ggacggaaag tcgtttcttt ccataaaaag gaattccgtc
    19981 cgcatcagac cgactccgtc ggtaaactgg gcgatttcag gtttgtaact attggatgag
    20041 ccgatgttta cggccaaatc gattcgcgtt ttgtccgttg tgaacgggat tcttttcaga
    20101 atatctcttt cttttttttg ttttgtttca taatttttct taagatcgtt atatttatcc
    20161 aatgtcgctg aatcgggatt ttcataaatg gttccgttgt acccgtcgat aataatctca
    20221 tcgccttgct tgatcatatc ggtaattccg gttatcccga atactgtggg tattttcaaa
    20281 cctttggcca tgatcgcgac atgcgatgtc gcgcttcctt tcgaaagtgc gattcccttg
    20341 atatagtctt tggagacaga tgccagatcg gacggaaaca attcttcggc gatgagtatc
    20401 gttccttctt cataaaacat gttagcattt ttgtttttgt cgccatctat cgtatcgatg
    20461 aacatttttc ctatgtcttc gatatctttc gcccgttcct tgatcagttc atcgtcaatc
    20521 tgttcaagca tatttgatat ctctttaaac gaaacaagca aggcgcttcg gcaggtataa
    20581 tttttttgct ttatcagaag acggactttc ttttccagtt cgggatcctt gacgatttcg
    20641 gcgtgagcgt cgaatatttt cgcttcttcc ggattttttt tcttcatgtt gctttgcaat
    20701 tcgttaattt tttcaagaac tgtctgacgg gtcgcttcaa ataaagcgat ttccttttcg
    20761 tattcgtttc cgatgatata agcaatgttt tttgtatcga tactatcccg aaataaaatg
    20821 ctggctttgc cgatagcgat gccttctaca atcgtgacac ctttgctgta ttttcgcata
    20881 tttacctccg cccggaatcc atccatacgt taaaagcaaa taaaaaaccc aaagaaaaat
    20941 tttcgatggg ttacgcctgc aataaatcag taacattccc tttatagaca cattctatcg
    21001 cttttgattt tttttgtcaa gtccatattc atcgacagat ttgttctttc aaattcttaa
    21061 tttttcctga tcctgccatt tttcaattta tcgacaatgg atacgtatcc cgctttcccg
    21121 attattacat ggtcagccaa aaatatttgg aggatttctc cggcttccag gaaacgtttc
    21181 gtcatctcta tgtcgttcgc tgacggttca ggatccccgg agggatgatt gtgagccaaa
    21241 attatctgat atgcggaata tttgacggca tagcgaaaaa cttcgcgagg atgggcaaca
    21301 gactggttca agcctcccac gaatattttc cttttggcga tcaaattcga tttaaggtcc
    21361 agatataggg cataaagaac ttcttgtttc aataatccga tctcgtcttt caaaagtttg
    21421 aagacatcgg caggagatct tatcggaaca gtttcccggt tttcgtttaa cacccttttc
    21481 ccgagttcca cggccgccaa tagttttgtc gctttggaaa ctccgattcc tttcgttttc
    21541 atcaactctt ccgccgtcct gtcgcgaagt tcaccgattg tttccagatc ggaaagaatg
    21601 tccctggccg tttccaaagc ggaattattc tgactgccgg tcgatatgac gatcgccaga
    21661 agttcgtgga tcgaaagatt ttccgctccg atcgtcaaca gtctctcccg aggccgttcg
    21721 gaaccgggaa tgtccttgat catatacatg gattgtcacc ctgttaaagc atacgcgctt
    21781 tacggattat ttctttgaaa aaagaccggt ttcccggtct ttaatctatt tggcgataag
    21841 cggtctaatg tccgatacca gatacaagga cccggtcacc aggagaatct ggttttcctt
    21901 taaatgctct cttagttcca gaaaggccgc tttggcgtca tgatgaagga acttattatg
    21961 atgatggctt tcttggaata gcgtttcggc atctgcgcgt ctcggatact cgatctcggc
    22021 gaaatgaaat tcatcgacca cattgtcgag ttcctggatc actttctcgt gctccttgtc
    22081 tttcatcatg cagaaaaggc agatgatata tctatcggga aaaatatctg ctaaagcttt
    22141 tttgagcgac agcacggcac cgatgttatg ggctccgtcc aaaacaatct gatgctcgaa
    22201 tatctcgaac cggccgggcc acgaagtcac ggcaagaccg tttctgatat tgtcttctgt
    22261 tatcgtcaga ttgtctttga gcctcaaaat acggattgct tcgattgcca gcacggcgtt
    22321 tttaacctgg tgcattcccg ccattttcag acggtattct tccgttctgt acataaatgt
    22381 cgtttcttcg ccgattttca cattggaaat cagggacaga tcgacgattt tcatcctggt
    22441 attatttttt gcgaccacct cggaaaacaa ggggaacaga ctcttgtttt cgatcgaggt
    22501 gacaagagga ataccatctt tcacgattcc aagtttgtta agtgcgatgc tttccagggt
    22561 gtttcccaat tgtttcatgt ggtcatacga gatgttggtg atgcaactga cttccggagt
    22621 gatgacgttc gtcgcgtcaa gcgtgccgcc gagtccgact tcgatgacgg caaaatcaac
    22681 tttttggtca cgaaaataca tgaatgccat caaggtaatg atctcgaaaa aagtcagggc
    22741 ttcgttgtct tctgtcaaga gacggtcgcg gaaattacgc aggaaagagg cgtattttac
    22801 tacgtcctcg tccgatatga attcctgtcc gatgccgatc ctttcattga atttcaggac
    22861 aaagggggaa gtatagactc caaccttgaa accggcatca tccaggatgt tcttgatgta
    22921 attgaccgtc gaaccctttc cgttggttcc ggccacatgg acggatcgga aggaattttc
    22981 cgggtgattc aatttggcgc aggctaggcg catcttggat agatcgagtt tttccccgaa
    23041 acgtttctgc gtctcgatcc attccctggc ctcggtaaaa ttattgaaca tttcctattc
    23101 ggtcagctcc tgcaacctgg ctttggtttc agccagttgt ttgcgataat tctcatgttt
    23161 ttccttttcc tgggcgattt tggcttccgg agcctttgcc aggaacgatt cgttcgttag
    23221 cattgcgcta cttcggtcga tttctttttg aagtcggtcg gtttccttgt ttagtcgact
    23281 gatttccgca tcaatgtcga ccagacttcc gagcgggata aaagcaatga gttccggcat
    23341 gcttatcatg atggcccgtt tgtggttttc tgctttttgg gtaaatacga ttttttccgg
    23401 attgagaaat ctttcgatgt attgactgtt ttcttgggtg aatgtcctca cttccggatc
    23461 gcattcgata tttatggcga ttttcttagt atagggaaca ttgtattcgt tcctgatctg
    23521 acgaaccttt ctgatcagct cgaaaaagta ttcctttccg gatgtttccg gataactgta
    23581 ttccgctgtc ggccatttgg cgatcgttat tgataaaatg tcgtgcggta tccgctgata
    23641 gatatcttcc gtcacgaaag gcatgaacgg atgcaacagt ttgataatcg catcaagaac
    23701 gtaaagaagg acggatttag tcgtggcgga gtcctggttc agtttgctca tttcaagata
    23761 ccatgcggcg aaatcgttcc aggtaaaatt gtagattgcc ttggcggctt cgccgaagtc
    23821 gaaccggtcg aattggcggt cggcttcttt tattgtttcc gccagacgtt cgagaatcca
    23881 tttttcggca aatccgagcg aatcgctttt gacgacgata tcttcttttt tcatgccgtc
    23941 ggtagtcatg atgacataac ggctgatatt ccagagcttg ttgatgaaat tccagctcga
    24001 ttcgactttt tccgtctcat aacgcaaatc gaatccgggc gcggagtttg tcgaaatgaa
    24061 ataacgaagg ctgtcggccc cgtattccct gacaacatcc tggggatcga cgccgttatt
    24121 ggatgatttg gacatcttct ttccttcgct gtcgcgaacc aagccgtgga taacgcattc
    24181 tttaaaggga gactgtttgg taaaatggat tccctggaag atcatgcggg cgacccagaa
    24241 aaagatgatg tcgtagccgg taaccatgac actgttcgga taataacgtt tgaaatctgc
    24301 ggtttcatgc ggccatccca atgttgaaaa cggccacagg gcagaggaaa accaggtatc
    24361 gaggacatcc tcgtcctgtc tccagccttc gccgggggat tcatgcccgc aatagatttc
    24421 gtcatcctta taccaaaccg ggatccggtg tccccaccaa agctggcggg agatgcacca
    24481 atcctggatg tcttccatcc aatgttcgaa tattttctgg aaacgctcgg gaacgaaagc
    24541 gacttcgctg ttttcaagcg ccttgtcggc gagatccttc atggccacaa accactgctt
    24601 cgacagccgt ggttcgatga tgatgccagt tctctcgctg tgtccgacgt tatggatgat
    24661 cggttcgatc ttttgcatca agccttgttt ttccagttct ttgacaactt ccttacggca
    24721 ttggaaccgg tccatgcctt cataaggtcc agccaggctg ttcatggaac cgtcctcgtt
    24781 catgcataag ggcatcggca gattatgcct ttgtcccacc aaaaaatcat tgggatcgtg
    24841 ggccggagtg actttgacga taccggttcc gaattcacgg tcgacaagca tatcggagat
    24901 gactttgatt ttggtgtttg tcagaggaat ataaacttct cttccgatat attgccggaa
    24961 tctctcgtct tccggatgta ccatcagggc cgtgtcggcg aacattgtct cgggacgggt
    25021 tgtcgcgacc gtcagtccgc cgtcggaatc ggaaaacttg tatgtgtaat aatacagatt
    25081 cccttggatt tccttatgtt cgatctcgat atcggaaagt gctgttttgg cctcgacatc
    25141 ccagtttatg atccggacgc cgcgatagat gtatccctgt tcgtaaaggg tcacaaacac
    25201 tttttcgaca gcttcggaca gtcctttgtc gattgtgaac ctttcgcgcg aatagtcgag
    25261 cgagagtccc atgctctccc actgctttct gatgtatccg gcgtattctt ctttccattt
    25321 ccaagcaacc ttgagatatt cttcccttcc gatgtcaaaa cggcttattc cttccatttt
    25381 caacttttgg tcgaccttag cctgagtggc gataccggca tggtccatgc cgggtaggta
    25441 aagggcgtcg tatccctgca tccgttttct gcggatgatg atatcctgaa gcgtattttc
    25501 gaaagtgtgt cccagatgga gtttcccggt gacgttgggc ggcggaatca cgatacaaaa
    25561 aggcggcttg ttcatattgc cggcctcgaa gaagtgatta gccaaccaga aggcgtattt
    25621 tcccttttcc acttccagat ggtcgtattt ctgattcatg tcattcatgg tattccttcc
    25681 cttccaccag ggcttccttg cccgcatagt aagcgtttat cgtttcgtag ttgtcgacag
    25741 aatggatcct tggcaactta tctttggaaa agtattgcat ttcaagcgat tcgtggtcgt
    25801 ttacccgcag gcttcccccg acgattttgg cagcgaaggc gaaggctatg acccttgctt
    25861 cgtcatgttc ccgccagcgc atcatgggat tgttaaatat acccagaaaa cgggtgagtc
    25921 tgatcttgag gaatgtttcc tcgtagactt cgcgaattgc gcattcctca atcgattcct
    25981 cgagttccat caatcctccg ggaagacccc agagatgatt gtcggatctt ttttccagaa
    26041 gcacttcgtt tttctcgttt acaatgacga cggaacaagc gcagagtatg actttgttgt
    26101 cgccgaccat gtttctcaga tatttgatgt agttgtccat tgtcgtttct cctttgcaaa
    26161 agaaaatcgt ccttgtgaaa aggacgatct gttatatcgc ggtaccacct ttgttccgga
    26221 aaattccggc tcttaagccc ttaacgcggg aaacgtccgg gattactata tattcacccc
    26281 gacaactccg tggcgacctt cacctttcct tctggctgac ttgcaccctg cgtccgctcg
    26341 cttttttccc ggattggcta ctcttccact tcactgtatt tgaattgtat tttatcagat
    26401 aatcgtcgat ttgtaaagcg gattcgccat aattgtcgac gatcattacg ccggcgcagt
    26461 aagatatttt catgaagctt agcgcaaggc ttccgtccat cacttcgatc agttgggaaa
    26521 atacatggta gtgctcagtt tcgtcccata attcggcgca tttctggtgg cactgttcct
    26581 tttcccgaaa gttagtgaac atgatgtccc cgatcagaat cttcccgtat ggaagcagat
    26641 ggggaatgag gtattccacc atatcgacga agtgttcgat gtctaaatga tggaaagcgt
    26701 atgtcgcgac gatgaaatcg tatttttcct tgttgaaatc atcgggaagc cccaaacaga
    26761 aatcggcttg agccaagttg gcgtccggta gtctcaagcg ggcgatgtca agcatttttt
    26821 cggaactgtc cattgcataa agagcatatt tttccggcgg cagtttctga tataatgttc
    26881 cggtaccgac gcctaaatcc aggatcttcg ctttggctag ttgagtctcg tctgaaatga
    26941 ttccggctat cacgttcata agttcgttgt atccggcgaa cggaaattga ttttcccggt
    27001 cggcttcctg gaccatctga tcgtattctg acgccatttt gtcaaatagg ttttcagtca
    27061 ttcaatccca cctctttagc aatgctcttt tcgatgatat tccatataag ttctttgttt
    27121 tccccggttt tggcggaaaa cggaacatag gtcccattga cgtcgccgat tgtttccttg
    27181 atcagcttca atgatttcga ccgttcattg ttcgacagtt tatcagattt ggtcaaaaca
    27241 taacaaaccg gcaatccctt ggcattgaaa aaatcgcgca tgacgatatc gtccttggat
    27301 ggcttgtggc ggatatccag aagaaaaagg gccaagacga cattgcgttt tgaattcaga
    27361 taatcagcta cccgttggcg gaacgtttcg acatcttccc ggcttccaag ggcaaagccg
    27421 tatcccggca aatccacgag atggaattct ttgtttatga gaaaaaaatt cacaagcctt
    27481 gtttttcccg gtgtctgcga caccctggcc atatgcctgt tgttcaacaa ggaattgatg
    27541 aaagaggatt ttccgacatt gctcctgcct gcgaagagga tttccggcag atcgccgtcg
    27601 ggatactgtt ttttgagggc cgcggtggcg gctagttcga tcgaacggat gatcatcttg
    27661 tgctcctttc tcagtatgac agaatccgga gaatcaggaa tttaaggtac atggtctcat
    27721 cgctgccaag caagacgggg tgatcgctgt tttgcagcga cagttttacc atttggacgt
    27781 ttttctttct gtcggcggcg gcttcggcca gaatatccag aaaatggtta agctccagat
    27841 aatgagaaca tgacgaggtc acgagatacc cgccgtcgtt taaaagatcc atggcctgca
    27901 tgttgatttc cttgtatccc ttgcttgcct gttccacatc gctttttttc ttggcaaaag
    27961 ccgggggatc caggatgatg acatcatatt tcgctttctg cctgttggcc atgcgaagat
    28021 attcgaacac atctgatttc acaatatcga tgtcgagatt gttaagcttg gcgttttccg
    28081 agattctctg acaggcatcc tgggaaatgt cgacggcggt ggcgcggcaa gcgcctgcca
    28141 atttcgcctg caaagcaaaa ttgccgtcat gcgagaaaca gtcgagtact gatttgtctt
    28201 taacaaacgg aatgatcgac agatgattgt ttctttggtc gagataagtt ccggtcttct
    28261 gcccgttttt tatatcgacg tataatttga tgtttccttc ttttatcata acacggtcgg
    28321 gcacaactcc caataacaag ttcttaattt gttccaatcc ttctttttca cgagccttga
    28381 catcgcttct ttcatagatg ccctttggat tgtatatatc attcaatagt tgaacaaaat
    28441 cggcttttct cttatcggtg ccaagcgtca gaaactggac ggatagataa tccgaatact
    28501 tgtcgacgat caatcccgga atcccgtccg cttctccgaa gcatgcccgg tatgtgtcgg
    28561 ttaatccggc atcctcccga tgtgttttcg ctgcttccag tctttttcta aaaaaaacgt
    28621 tgtcgattac atcatcggga tttcttgata aaatccttac gaaaatcttt gaagccgtgt
    28681 tcaaaaaacc ttgtccgaga taatccccag aaaaagtgta gacatccgac aggttgccgg
    28741 attcgatcgg tcccgttatc cggtttactt cattactgta aacccagaca tggccacgac
    28801 ggatccgttc atcctctccc ttattcagat ataccttgca ttcgttcata tatattttcc
    28861 cttcatccta gcggacgccg taatggacga aagcctgcgg catcagggcg tatttgtttc
    28921 ctgtttcctg taaatccttc ggagtcatcc ggttgatggc atccatgatc acaaatatat
    28981 ccgctcccct ggcaagatat tcgcttagaa gagaacatga tcgggaaacc gagtcgaagg
    29041 caagtacgaa atcgccgatc atttttcttt tctgtctttc caaatcatcc ggtttgacaa
    29101 tgtgttccct tgtcaatgca aacagatttc gaaaagcttc cattgtttct tccggttttt
    29161 tggtttcgct atacaagata atgtgtccgt aattagttgt gacggaaagt gaaaagtcga
    29221 aagtgtcgtt caccagacct tgcttctgca attccagttc cctgccgctc atcttgccca
    29281 tataattctc caaaaacaga taacctttta tttcttctag ggcagcttcc atcggtgtcc
    29341 ttaagactga attctccagt ttgatcccca tcatcacgat actgttgcgt agatcgcgtt
    29401 tctccgtata atattccctg gcaaggcctt caggatcgat tttccgaatt cttttgaaat
    29461 ggtcatttac caagacattg tctgatgcca aattttcgag gagcgtaatg attgcttcct
    29521 tatcaacgtc tcccgctatc accaatatca tgttttgata acgataaaag catgaaaatg
    29581 ccttttccag agtgcctgcg gaaatcgtcc gtaggctttc ctttgatccg gcaatgtcgt
    29641 ttttaaccgg gtttgatcgg tatagggaac gcatcagatc aaaatatagc gactgctcga
    29701 catcgtcgtc gtaaagggag atttcttcgc tgacaatttt cttttctttg tttatcgcat
    29761 cttttcggaa atcaacgttc tcaagcattt cgaaaagcaa tttcagaggt tcgtcgatag
    29821 ccccgacggt tgaaaaataa atggctgtcc ggttgttggt cgtataagcg ttggccgtag
    29881 aaccgatttc ggtgaacttc cgtgtgtaaa agccatcttg gctttcgaat aagaggtgct
    29941 caagaaggtg cgccgatccg gcgggaaaac tcattttctc gtcttggcat tcgatgtttt
    30001 catcttccga tccaaaggga attattaaag tgacggcttt gcggaaaaaa cctttttttg
    30061 gcaaaactac aactctaagt ccgtttgtta gcctggtgtc aagcattttt tccccggata
    30121 accacgtggc gttgttaatc gtcataatat tcccctcctc ttgttcacat agactgtatc
    30181 gagaaaaagg gatgaatatg cttgacggac atcttccttc gacacccgtc tgatgtcttc
    30241 gatcgacgat tgcatatcgt attctttgtt atagaacaga tcgtaaaaaa agatccggtt
    30301 gactgtcgaa cttggagaat cggctgcctg atgaattctt ttcacgatga cttccttggc
    30361 catttccagg tccttttcac tgatttcccg gtctttcatt ccggaaacaa tcctttttgc
    30421 cagttccttg gcttcgattt cttttcccgg ctcgatggca gcgaagacaa aaagaacgcc
    30481 tttgtctccg atataattgg aaaacacatc gtaaccaaga tgacgttttt ccctgagttc
    30541 caagaacaaa gacgattggt cggaatcccc caaaagcacg ttagcgacga taagcgcatg
    30601 gtaaagggga tgccccggaa cgatttttgt ccggtaaccc atgtagattc ttgactgatg
    30661 gacatcggcc aaatcttccg attcggtagg aatatcgggt aatgttcctt cgtcgtctat
    30721 cggacgtaat aacgaatcat tattgccgta cttttcaaga tcttcgagaa tattcaggta
    30781 tttccgatca aaatcgccta tcgaataaat cattatccgg tcattatcga tcatatcatg
    30841 atatgctttg acaaggcttt ccccagtcag attgtcgatc gtttccaatt ctccacgtgc
    30901 ggaaaaccgg aataattcct tgggaaacat ccgtttgcgg aaacaatcgt agccatagag
    30961 aaaccggtcg tggtaatcat tttcaagcga ttcccttaat aagcgttttt ccagtttgaa
    31021 aaggtctttg aaataatcgc atcctccgaa atccggttga aaaacaagtt cttttagaag
    31081 cgacaacgcc tgaggaaaaa tcaccggatt accgggaaga tgcttctcgg aaggaaaaat
    31141 caaatcgaaa gagatcacgc tctgaaatcc gatttttctt gtggatgtga cgatttcagc
    31201 tccgtacagg cttccgagtt cgcgctgaat cattgtcctg tctttaagtc ttttgtttcc
    31261 gccagtgagt atttccggta aaagtgctct ttcgttcaga ttgttttctt tgaaatcgcc
    31321 gataaaacaa agcgatatct ggacaccttt gaacttatcg gtatgtatgt ctcttatgcg
    31381 ggtctgtccc gggtttgttg caattttgaa catcgatgtc tccttataga acaatggatg
    31441 tttatcggcg ataaacatcc atgcttttca ttccaactcc agggcaacct tcatcatgtt
    31501 ggtaaaagcg gtttcgcgtt cgcctgccgt cgtttcctgt ttggtcacga acgaatccga
    31561 gattgtcaga aggcaggccg cctgtttgtc gagaacggtg gccacatgga aaagagcgaa
    31621 tgcttccatc tccacggcca gacatttgtg tttgtttacg aaatcaagat agatatcggg
    31681 attttcgcgg tagaacacgt cgcttgaatg gattcttccc tgagccaggg atattcccag
    31741 ccggttcgcg ttttcgatca attttagatt aagttcaggc gatgatttca aaatatgctt
    31801 tttgtatccg ttttgggcat tggcaaagtt ggaatcactc catgcttcgg tcacaagaag
    31861 cgtatcgaac agtttaagtt tcggcgaata ggctcctgcc gatccgatgc ggatgatccg
    31921 ttcgacattg tagaatttga acagttcata ggaatagatt ccgatcgaag gcattcccat
    31981 tcctgaaccc atcactgtga tctccttgcc gttgtatttg cccgtgaatc cgagcatgtt
    32041 cctgacgcca tttactattc tgacgtcact cagaaactta tcggcgatga atttggcgcg
    32101 caagggatct ccgggcatca aaaccgtcct ggcgatatcc tgcaatcttg cttcaatatg
    32161 cggtgttggc attatgacac ttccttttca ggttcgggta tctgaatcag cgtctgcaaa
    32221 agggagacga actcttcgtc aagcggtatc aagagatcgt aatacattcc gcttaccgat
    32281 gattttacat gataatattg ctgtttctgg tctttttcga tccggtgaat gacgaactcc
    32341 cgtccagcga cggcggtctt gttcctggtt tctgtttctt tgaccttctg agacgggaat
    32401 atctcgagga agacttcgcc gttcaataaa taaaggccgg ctttgcggac gaataaggaa
    32461 agaccgcttt ggccaagaac tggcacatac aattccattc ccggtttgtt tttaagcagg
    32521 aactgatatt gggaaaagac cttttttttg gcttgaaaat tcatcatcag atcgcgggcg
    32581 aacatataaa tgagcaagag accgaaaaaa atcagaatgg tatagatatc gattccgaat
    32641 tcaaaaatcc ggtccatgac atacaaaatg acggcgaaaa gcacggtgtt ggtgatattc
    32701 atcgtatatt tgatggggga ggccagttta atggcggaat ccatcagtgc tttttgacgg
    32761 ccatcttcca tcagtagccg ctcccggttt cacccttgac caaggcaacg ccgttcgacg
    32821 tcccgattct ggtggctccc gcctccaaca ttttctctaa atcttccttg gtacgaacgc
    32881 cgccggcggc tttgacttcc ttgccgttac aattatcctt catcaatttg acattttcca
    32941 tcgtagctcc ggtgggtccg aatccggttg acgtcttgac gaatgcggct tctgttttgc
    33001 cgaccagttc gcttgccctg ataacctctt cttccgtaag ataacatgtt tcgataatca
    33061 ccttgacggt gcggccgaga gaagccttga cgacatcttc gatttcctgt ttgacgtatt
    33121 cgtcgtttcc ttccttgaac gctccgatat tcatgaccat gtcgatctcg tcggctccgt
    33181 ttctaatcgc gtccagggtt tcgaaaactt tggtttccgt agtattggct cctagaggga
    33241 atccgatcac cgtacatacc agcacatcgg tgtttaaaag ctgttccttg caatacttta
    33301 catggcaagg atttacgcaa acgcttttga aatcgtactt gatggcttcg gccaccagag
    33361 tttcgatttc cttgacggtt ccgaatgctt tcaggtatgt atgatctatc aatttgttta
    33421 tcttcataaa acatgtctcc ttctgtatac aaacgccgtt aaatcaaggt tatatgatgt
    33481 ttgttaatgc tataatagta tactttaata tgacataaaa gtcaatcaaa gcaaaaggaa
    33541 aagccgacaa gcggattgcc ggctttctct tattctttgt tattttaaaa gcacgtcgtg
    33601 acaacgcggg caaagcccgt tttcatcggt atctggaaca gtcatccaac aacggtcgca
    33661 gacgactcct tcacagggaa agacatcgac cgaaagaccg ttttcgtcgt atgatccttt
    33721 tccttcggac ttgatttcca gcttggaaac aatgaagacc agttggagat tgcattgcaa
    33781 gttgtcgatc aggttctgat attcttcgtt agggtacaaa acgacgcgag cggccatgct
    33841 tttcccgatc actttattgt ttcttgccag ttccaacgct ttaaggacga tttcgcgcat
    33901 ttccatgaag cgatcatatt tatctatgat ctccacggcg tccggatatt cttccggcat
    33961 tgatatatcc gtcaggtaga catcttcctt ttcgcggtgg gggatcagat cataagcttc
    34021 cgatgcggta tgcggaatta tcggggtcat gatgcgaagc agactgtctg tcgcgtgata
    34081 gatgacggtc tggatctggc ggcgtcttag gtcatcggct ttttcaatat atagaacatc
    34141 cttggagaaa tcaagataga atgcggacag aaaaacgaca tagttgttta cgagccggta
    34201 gacatcatcg aagcggaaat cgtcgtaaga tgccaatact tccttcatga gaccgtctgt
    34261 cttcagaatt atgtatttgt ctatctcttc caattgtcca tagggaactt tgtttttggt
    34321 ttcatcataa tcaaacaggt tccccaaaag aaatttgagc gtgtttctga ttttgcggta
    34381 agattcggaa acttgtttca aaagatcgtc agacaatctg acatccgcct ggtagtcgac
    34441 gctggatacc caaagacgat agatatcggc tcccatcgtg ttgcagattt tgttcggatc
    34501 aacggtattg ccaagcgact tagacatttt acgtccttcg ccgtccaaca cgaagccatg
    34561 ggacaccagg gttttgtatg ggctcttgcc ttcagtggcg atacccgtga tgagacttga
    34621 attgaaccaa ccgcgatatt ggtcggaacc ttcgaaataa agatcggccg gatacggaaa
    34681 tcctctttcg atcatggcac catgatggct ggatccggaa tcgaaccaga catccatgat
    34741 gtcggtttct ttccggaata tgccgtttgg cgattcttca ttaacatatc cttccggcaa
    34801 tagttccttg gcactcattg tgaaccaggc atcggagcct tccttttcga agatcgtggc
    34861 gacatgttcg atgattgtct tgttaaggat cggattccct ttttcggtat aaaacgccgg
    34921 aatcggaact ccccaggccc gctggcggga aatacaccaa ctgcctctgt ctttaatcat
    34981 gttggacagt ctcacgtcgc cccatgaagg ataccatttt atttttttga tggcatccag
    35041 gatatcctgt ttgagaatgt cgatcgaagc gaaccattgc ggagtcgctc ggaaaataac
    35101 cggtttcttg gtccgccagt catgcggata actatggacg atttcgactg attttaacaa
    35161 gcttccggct tcgtcaagag cttttatcac ttcggggttg caatcatcta cgaacagtcc
    35221 ttcgaaccgt ttgccggctt cttttgtcat gcgtccgagc gaatcgacgg gacaataggc
    35281 ttcaagattg tatttctgcc cgaccaagaa gtcatcctcg ccatgaccgg gagcggtatg
    35341 gacaaggccg gttccatcct cgatcgtcac atgatcgccc aagatgaccg gggacacgcg
    35401 gtcataaagc ggatgtttgt atgtgattcc ttcgagttct gatccaatgt attcatgcag
    35461 aatttctgtt ttttcccaac cgagtatgcc tttcaactgt tctaccaagt tctttgcggc
    35521 tacgtatttc tttttgttat cggttgtgaa aagagcatat tcgatatcag gaccggcgca
    35581 aatcgccaga ttggcgggaa tcgtccaggg agtcgtcgtc cataccagca tcaaagcgtc
    35641 gtcaggcaaa attcctttcc cgtccacaac cggcatcgac acataaatgg aaggggactt
    35701 aacatcgtgg tattcgattt ccgcttcagc cagggcggac tcgctggaag gcgaccaata
    35761 gactggcttc aggcctttat agatcaatcc tttttcaacc atttggccga agagtctgat
    35821 ctgggcggct tcgaaacgtt tgtccatggt gacataggga ttttcccatt cgcccaacac
    35881 acccagacgt ttgaactgcc ggcgttgaat gtcgacctgt tctttggcat attcggcaca
    35941 aagttttctg aatgcgacca cttcgaccga tttacggtcg actttccggt ctttcgacaa
    36001 ggcattttcg attggcagac catgggtatc ccatccgggc agatagggcg ccttatatcc
    36061 tgccatgttc cggtagcgga tgacaaaatc tttcaagatc ttgttcattg ccgtgccggc
    36121 atggatcgat ccgttggcgt aaggaggacc gtcgtgaagt acatacaaag gacgaccttc
    36181 gtttttcttc atgacttttc tgtaaaggtc caaatcgtcc catttcgctt ggatctccgg
    36241 ttccctttgt ccgagattcc cccgcatggg gaaatcggta ttaggcatca gaagcgtgtc
    36301 tttgtaattg ttcttgttat tgtccattct aaaaacctcc tgaataaata aaaaagacct
    36361 tcctctctga ggacggtctt gaccgcggta ccaccttagt tccaggaatt cctggcgctt
    36421 gtcgtcttta acgcagaccg gcgggaaaac ttactttgta gtcttccgct cgcgagtgat
    36481 atccataccg gaggatcatc cctgttcgca ccgaccacag ggtctctttt ttttccgacc
    36541 gtaaggcatt gtctcgctca ttgctttcat ttgaataaat tataatccaa tgtatgatgt
    36601 ttttcaagta aaatcttcct ataacactct tgcgattatt ccgaggatga tctgatagac
    36661 gatgagaccg atcatcggag tgaaatcgaa cattccgatt gtgagccaat tgcggaaata
    36721 tccgagataa agcgatgata ttctgtcgag gacctggtat gtccgcgtat ttcgcacgct
    36781 tgtccaactc aaaatgatcg ttacaatcat gacgacgaaa taaacttgca atatggaata
    36841 taggattgtc aggatgtaat aaagtatttc cggcataaac tcacttcttc gtataatcgt
    36901 ggatgaactt ttgcaaagtc ggccctttca ggtctgtttt ttgcgccatc agatagattt
    36961 tgtcttggat tttaacgatt tcgccgtcac aggcgtatag tactccggtt aagaacatca
    37021 gtacttcgtt ggcttcggtt atcagacagt cggaaaaatt gaggacgacc ggtaaccggt
    37081 gcatgatcat ttcggcgtat tcatagatgc cggaggtatc ctggacacgg taaaacttca
    37141 agtcttcgaa actgatgtca gtggccacga ttttctcttt ttttcctaac attcccattt
    37201 tcattccccg tttctgtaaa ggatccttcc gagccgcaga tgagttgccc cctctgacat
    37261 ggcgatcctg taatcgtcgg acatccccat ggaaaggtaa ttgcagtcaa cacccgggat
    37321 tttcttagaa cgcaagtcat cccgtaacct agcaagcagg gcgaaactgt tcctgatttt
    37381 cccggtgtcg tcggtaaggg gagccattcc catgagtccg atcactttta ttttatcata
    37441 ctttgcgact ttttcacaaa aatcacgcag atcttttggt ctgacgccgg actttgattc
    37501 ttccggtgat atatggactt cgatgaaaca tggcaatacc tctatccgtc gcttctgaat
    37561 ttcctcggcg agactcagac ggtcgagcga atgaaggcaa tcgatctcgt tgatcatttt
    37621 tgccactttg ttcgtttgca gactgccgat aaagtgccaa acgagcttta tatcggataa
    37681 agcttccttt ttggccagaa aatcgttgac tctgttttcc ccgacattgt taatcccggc
    37741 ttcataaagt tcgcgaattt cggccaatcc cacatatttc gtggcagcca cgatcgtttt
    37801 acccttggtt tcttccaaaa gtcggatggc gttttcccgg atgctatcag gcatcgtaat
    37861 cacctcagtt tttcttgcgg atgtaatatg aaaaaaaggc ggacagaatg acgagataaa
    37921 tcgaaatcgt gatcacatgg gaaatgttcg ccttatagat gacgatcaac acgaccccgg
    37981 cgatgacatt tgccatataa agcgaaaaag agatccaaaa attacgcttt tgtttcttgt
    38041 cactcatatt gagccacttt ccttttcaac tcttccagtc ccttgtcggt ctggaaaccg
    38101 aacttgagag gagtaatcga tatgtagcca ttatggacgg catggtagtc ggttccttcg
    38161 acaaactcgt tgactaagtg atgccggcgg gttctgtaat gaccgttttc ggttttctcg
    38221 taagtgtact cgaacgggta gtaaccgaca tcggtgatct ttatgccgtt tgatttgtcg
    38281 aaacgcttgg aaacgaaatt gacattgaga atatagtcgc tcgaaacaag atcgtgagcc
    38341 agaataaaat ccatcgccgc ttccgtttcg gcttccacgg ggctataata cccaaaatcg
    38401 gtagaaaagg caatgcattt gtatccgtct ctcaaagctt ccatacaggc gccgacggta
    38461 ccggaataga ttgtgtcggt tccgatattg aatccgtcgt ttattcctga tacgactaga
    38521 tcgggattca gcttcaatcc atggatggca aaagccacgg catcggccgg ggttccttcc
    38581 acgctgtaaa tgtcgtcttt gtatttcgtg acaatggctt cattccagaa aatccgggaa
    38641 accgaggctc cgctcatgtg ctttgccggg gccaccagag tgacgtcgcc gtattttctt
    38701 agtttttcat aaagaatccg gatgcctttg gcatcgtatc cgtcgtcgtt tgtaagtagt
    38761 attttcatct tttcaactcg tttcctgtaa tttttacaat gcctggtata tcggcaataa
    38821 tatcgccagg ctgattgaat aatagatcga tagggtgatg atgtcgctca cggtcgtgat
    38881 gaaggggcct gatgcgacgg ccgggtctat tttgaccttg tccattccaa ggggaatcag
    38941 agcgccaagc aatgttgatg ccaggagcgc cacaaaaacc gaaaatgcgg tgacaacggc
    39001 ggtcgaaagc gagaccgcat cgaaagcctt tcctcgggaa accagattgg tgacgatgac
    39061 taggccgaaa acaagcacgc cgatcaccaa tccttcgatg atcccggttc caatctccct
    39121 aagcagatat ttacccatca cttttttcga tgtgtcccgt tcgggcgaat tcagatatct
    39181 gatcatgaca gccagactct gggtaccggt gtttccggac atatccagga tcaagggcaa
    39241 atagacagcc agtctggcgg ccaggacgat acttgtatta agcgcttctt cgaagaaaga
    39301 gaaaatcaac gatgttaaca aactgaggaa aagcacgatc gacagccagg gaagtctctg
    39361 tttcacggct cttgagaacg aatcccttcc caggtcgatg tcgccgtcgg ttaagccggc
    39421 cagtttggca taatcgtccg atttcgcttc ggaaacgata tccatcaaat cgtcatgggt
    39481 aacgattccg accagatgca tgtctatgtc cgtgatcggc atagacgatt ccccgtatgt
    39541 ctgcatgagg ttggaaacct cttccttgtc gtcggttgga cgcgcggtga tgaagcgcgt
    39601 ttccatgata tcgctgatcc tttcctccgc tcttgcgatg atcaggcttt tcagtcggat
    39661 atatccgacg agaaaattgt tttccaagac ataaaggatc gagatgtaat cggtgtctcc
    39721 ggctatcgac acgacctttc gcattgcttc tttcaccgtc atgttcgcaa acaggcggat
    39781 gaagcttccg gacatggcgg agccgatttc gtcttcttcg taaacaagta gtttattcag
    39841 ttccgcccgt tttttcgggg acaacagact gatgatatca atgtcctcgt ccttgggttc
    39901 caggttctgc aaaaggtcga cggcatcgtc cgtttccatg ttgtcgacga ttcttaccgc
    39961 tgtttccaag ggcaattgtt ttagtaaaac gatcgcatcc tccgaatcga aatgctcgac
    40021 aatggcagcg ccgaaaggat aggtaaaatg caaaaagaca tttcggcgcc tttcggaatc
    40081 tatttccagc aatgcctgag aaatgtcgaa cggatgatat tcctcaagtt gcttctgcaa
    40141 accggaaaaa tccgtttccg cgtttatgag ggtgacgatt tcaccgatgt agtctttttc
    40201 ttccaaacgg atttcttctt tttcgtccat cgtcctaccc ccttaaaagt atctcgccaa
    40261 caaggtggtg gcgaggccga aatagattaa cagcgatatg atgtcgttta ccgtcgtgat
    40321 gaaagggcct gatgcgacgg ccgggtcgat cttgaaaaga ttcaatacga gaggcacgac
    40381 ggatccggct aatgtggcga cggcaagcga ggccgacagg gaaacggaaa tgacgatggc
    40441 caagggcaaa acatcgacaa aagccatgcc ttctagcatc cgcattccga ttacaatgcc
    40501 gaaaataaga attcccatgg caaggccgtt cgcaatccca atcaatagtt ccctaataag
    40561 gtgcttgcga atggcttttt tgtcatccag ctgattggtc gcaaacagcc taatgacgat
    40621 acccagactt tgagtgcctg tgtttccggc caaatttaaa atcaagggca tgaagattgc
    40681 tagggtcaaa acccgggaaa gcaagtcttc gtaaccggta atgatcgaac ttgtcacaag
    40741 attgatgaaa agcaaaatcg ccagccatgg catgcgtttc ttgacggtcg tccagaccgt
    40801 ttcgcggtct tcgtcgatat tgatgtcggt aagaccggct agtttcgaat agtcttcgtc
    40861 tgactctttg ttcaaagctt cgaacatatc gtcgaaagcg atgattccca gcattttgaa
    40921 atcaccagtt acaaccggta acagaaagaa gtcatagttt ttcatcaact cgatggcgtc
    40981 ttccgtcttc tgccaggggg taacgctgac gattttgacg gtcataatgt cctcgatcag
    41041 ggtgtcgggc ttgtttccgg cggatatgat ctccttgaga gacaaaacac cgaccaaaat
    41101 acctttgtcc acgacgtaga tattgttgat gaattcggtt tccggtgctt tttgaaccat
    41161 tgtggcgatt gccattttga cagtgtctgt tctttttatt tcgagatagt tattgttcat
    41221 gatcgaaccg acgagggaat cgtcgtattc aagcattttt ttcacttcga tgcgtttttt
    41281 tgcgtcaatc acggataagt aggccatccg catgtcggaa tcgtcgatgc aatcgatgat
    41341 atcaacgaca tcgtcagtct gcatcccttt gaacactgca gtcatcacgg ctacaggagc
    41401 attgtccagc acatccttcg catcatgggg atcaagttcc gaaaacacgg cagaaagtaa
    41461 cggaaaatcg aggatccggt aaaaatccga acgttcttct tctgtcattt caagcattgc
    41521 gaccgcgaga tcatagtcgt gataattatc gagtttctcg cgcttttcca gtaattccgc
    41581 atttgatttc aaaacatcta ttatttcctt tgtgttgatc gttctgtctt cgttttccat
    41641 tttccggacc tcctttccgt ttcttataga ttttatcatt tttccgggtc atatacaacc
    41701 tgaaactcaa agaaaaaggc tggcaagcca gcctttggtc atttcttgaa cctttctttc
    41761 aaccaggagg gaatctggat atcttcctct tcatccgcct ttgtttttgg aagcggttcc
    41821 ggctcgcgga cattacggtt tttcttctgt ttttcctttt tgcccggttt ctgtttctgg
    41881 aagatttctt cgacaggaag gatatcgtcg ttcttgattt cttccgcttt ctccaaaagc
    41941 gtgtccttga acaggggatc ctcggagaat ccggtggcga tgacggtgac gatgatttca
    42001 tcctgcagat cggaattgat ggcggaaccg taaatcacgt tgatgtcggt ggtcgaagat
    42061 ttttgaattt cctcgatgat ttcgtttatt tcgtaaagcg atgcatcgaa gccggaagtg
    42121 atgttcacga tggcgtcggt cgctccgttg atcgatgttt ccaaaagcgg acttctgata
    42181 gcggcccttg cggcttccac agcccggttt tcgccttcgg cgatgccgat tcccatcagg
    42241 gcggttcctt tgtccttcat cactgttttc acatcggcga aatcaacgtt cacgactccg
    42301 gggacggcaa tgatttcggt aatgccttgg acaccttgtc ttaaaacatt gtcagcttcg
    42361 cggaaagcat ccaaataagg ggtgttcttt tcaactatgt acagcaactt atcgttgggg
    42421 atcacgataa gggtatcgac ataaggtttc aattcttcca agccctggat ggcgacgctc
    42481 atccgtttac ggccttcgaa gccaaacggc tttgtcacga tcccgatcgt gagacaaccc
    42541 atttccctgg caatcctggc gataaccggc gcggcgcccg ttccggtgcc gcctcccatg
    42601 ccagcggtga tgaaaaccat atccgtgtcc gccaacaaat ccctgatctc gtcttccgac
    42661 tcgatggcgg cttgacgtcc gatgtcggga ctggctccgg cgcccaaacc tcttgtcagt
    42721 gtttttccga gttgaaggcg aatatcggct ttggaaagtt tcaatacctg ggcatcggtg
    42781 ttcatggcga caaaatcgac gccttggaca tcgttttcga tcatccggtt gatggcggac
    42841 ccgccgccac caccgacacc aatcactttg atattcggtt tctgattgaa tttatcgttg
    42901 agatcgaaca tgttttacct ccatatccaa agccgcctga tcctgtcgca aacgtcgaca
    42961 aaaaaacgac atactctgtg acaataatta ttatacgtgg tcatcaatgc tttttcaata
    43021 tcaagtccgc atatttttgc ggggttttgc cattaccttg catttacttc agacgttttc
    43081 cgtctttgga tagtatcgtt tgatgaattc atctttgaaa tcgccaaacc ggtcttccat
    43141 gatcgccttt ctgatatcgg ccatcaactt tttcaaaaaa tacaagttgt gataggtaac
    43201 aagccgcaat cccaaaattt cgtcggtttt gaaaagatga cgaatataac tgcgggtgta
    43261 gctttggcaa accttgcagt cgcattcggg atccagaggg cccatgtctt cctcgtaagt
    43321 tttgtttttg atgacgactt ttccctccga tgtcatggcg cttccgtgcc tggcgattct
    43381 ggtaggtaga acgcaatcga acatgtcgat accgcactcg acccctttta gaatgtcatc
    43441 cggagctccc acgcccatca gataacgggg cttgtcgtgg ggcatgatgc ctttcagata
    43501 ttcgagcatc tcgtacatgt catctttttt ttcaccaatt gacaagccgc cgatcgaata
    43561 acctggaaat ccaatttcca tcaggctatc caggctttgt ttcctgaggt ctttgtagtg
    43621 accgccttgg acaatgccga aaagcgcctg ggtatccggt ttttcgtgcg catcttttcc
    43681 tcttttcgcc catctgacgg ttcgttccac cgatgctttc atatattcgt aatcagcata
    43741 gaaaggcggg cattcatcaa aactcatgat gatatcagcg ccaaa
//
LOCUS       DAOPJL010000006        43121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_926_length_43121_cov_10.734965, whole genome shotgun sequence.
ACCESSION   DAOPJL010000006 DAOPJL010000000
VERSION     DAOPJL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 43121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43121
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_926_length_43121_cov_10.734965"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLP02_01560"
     CDS             complement(<1..489)
                     /locus_tag="PLP02_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018369544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPF42189.1"
                     /translation="MNKFKVVSSYEPTGDQPEAIRKLTENLRSEMKEQVLLGATGTGK
                     TYTISKVIEQVQKPVLVLAHNKTLAGQLYSEFKSLFPENRVEYFISYYDYYQPEAYVP
                     GADLYIEKDAKINDEIDEMRHAATSSLLERKDVIVVASVSCIYGIGDPEEYKNGMVTL
                     RAG"
     gene            577..2256
                     /locus_tag="PLP02_01565"
     CDS             577..2256
                     /locus_tag="PLP02_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HPF42190.1"
                     /translation="MKTLLVAIDAKYIHKNVAVRLLKANCDFPVDLLETNIKENVDDI
                     FDKIVSFSPDIIGFSVYIWNITVITKLTERIKSSTKAIVIWGGPEVCYDYEYFLSRFS
                     VDFVAIGEGELTFNQFLHAINENSGFDHISGLAFKKENIVFATKPAETIDLTELKSPY
                     RFQDDIAELPYQIQYLESSRGCPFHCSYCLASLENKVRFFPLDKVKDDLLFLIRNGAR
                     TFKFLDRTFNIDNKRAIELADFIIKNHLPGNSFQFEITGEILSDEFIDFINLNAPKHL
                     FRFEIGIQSTNESSNLAVSRRQDNDRLFFVIRKIRQAGVIDLHLDLIAGLPYEDLDSF
                     SKTFDAAFSLRAKELQLGFLKLLRGTPIRKNADMLGYSFNQDPPYEITESRWISKNDM
                     KKIRQVEQALNIFWNKFFMTKTIEKIADVELSIFHFFTNLFLYCKRVGFVFHRYQLSD
                     VFQVLNDYLCKTNPSLHDLVLPLMKHDYLSYHKIKPKIWWKEDKLERREVFRTFLEKN
                     HDYKLDTLYKYGILATYGDLFLLAIYMPDHTSILLFGHDGVILSDLSETAL"
     gene            2340..3248
                     /locus_tag="PLP02_01570"
     CDS             2340..3248
                     /locus_tag="PLP02_01570"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPF42191.1"
                     /translation="MKTAIIYLFTGTGNTRIAGDLIAGQLAKHNTRTTIYELKQPFEN
                     IPDPNQFDMVGFGYPVHAFNTPRFFLDFVKKLPDTNKKTAFVFKTSGEPFALNNASSR
                     PLIRLLEKKGFVPVSDRHMLMPYNIIFRYPDAMVKQMYLHTGKMAMLSVNDILKGNHK
                     KFRFSPLTVISLYVFRLQWLGAKINGPWIHVDKNLCAGCSLCVKMCPAANITMENGFP
                     KFSSHCTMCMRCAFFCPSDAVRPGFLNGWRVNGPYQFENIVKDDSIPTSFVQKDTKGY
                     FRLFKNYYERTNKEIKELEKSKQKAC"
     gene            complement(3270..4220)
                     /gene="pfkA"
                     /locus_tag="PLP02_01575"
     CDS             complement(3270..4220)
                     /gene="pfkA"
                     /locus_tag="PLP02_01575"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPF42192.1"
                     /translation="MKIGVLTSGGDAPGMNAAIRAVVRTAISYKMEAYGVFDGYKGLV
                     ENKIRKLERNDVGQIINRGGTILGSSRLPEFVEKEVRQKGIDNLRSHGIDALVTIGGD
                     GTYRGAMALTEMGMPCIGLPGTIDNDIASTEYTIGFFTALDTAIDAIDKLRDTSFSHQ
                     RCTVVEVMGRNCGDLAIYSGIACGAEIVITRDTGFDKEEIFKQVHHAREIGKRHLVIV
                     VAENITDVRELAREIDEISEYEARACVLGYIQRGGTPSSYDRILASKMGSYAVDILKE
                     GYGGRAVYARSMNVGHCDIHEALSMKLEPSDLYGYLSKLN"
     gene            complement(4224..7286)
                     /locus_tag="PLP02_01580"
     CDS             complement(4224..7286)
                     /locus_tag="PLP02_01580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPF42193.1"
                     /translation="MIDFDLYIQTGYSFNGSLIGIDALVENAKKQGFRTLGIADRGRL
                     YGALKFHQACKNAGIKPVIGMYVEATAEIFRDLPLLLLAKNDIGYRNLVKISSQLSND
                     KDHVQLGNLKENSNGVICVIMADAGEVHRAVAGGDFSFANQIIRLIEENCRELYLGLD
                     LNDFETETQVASELVNLYKTVIMNKVLYMSKEDIVASRVLKAILKESSLEESDLLTEA
                     EAVYDFKSPARLDKMYGDYRKSINNTNIMISEINLEFEYGHLYLPKYPVPDGHKAAKY
                     LRALAEKGLEKRLESKKTKTKNKDIYRERLAHELKIIEGMGYEDYFLIVWDIVLYAKK
                     AGILVGPGRGSSAGSLVAYVLGIVDVDPLDFDLYFERFLNPERITMPDIDLDFPDDKR
                     DEVIRYVSEKYGKDHVSNIVAFGTFQGKSALREAGRVLGTKPVIIDEVTKYISETDNS
                     IVTFKHDNSEKYDYLMKTPEIKMLFDIAEKIADLPKHISTHAAGIIISEAPITDHVPV
                     QPGLLDLYQTQYEAIDLEKLGLLKIDFLGIRNLTIIRRVLEAVKENIGKTIDIYKLPL
                     DDVKTFALLREVRTIGIFQLESRGMMNLLGKMNIHEFEDISTCIALFRPGPMENIPSY
                     LRRLNNQEKIDYIDPELEPILKSTKGIIVYQEQIMQIAHVYAGYSLGEADVLRRAVSK
                     KKEETLVAERTKFLDKCRRMNRDETVSNRIYDYIVKFANYGFNKSHSVAYSLVAYWMA
                     YLKANYPGYFMSVLMDSAAGSQGATLEYIQECRKLGIKILPPRINKSRKYYQMEKEGM
                     RYPYLGIRNIGSVVADRLDQLQEEGEYKSFADFVIRAKDINVRVIESMIMAGVFDDFG
                     LTKQTMMMNLKQIMMLGSMNQSLENLGFVYVEYPEYDRDYLASMEKELIGFNLSFHPL
                     AKYRDGFRREGISLLSEATLREKQETTFAALLRKIKVITTKNQEEMAFLEFEDMFVSL
                     EAILFPRDYRNYKNTLEKGGIYIVRGKIELRNNVQQMVIESMKPIKEE"
     gene            complement(7336..8655)
                     /locus_tag="PLP02_01585"
     CDS             complement(7336..8655)
                     /locus_tag="PLP02_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42194.1"
                     /translation="MLARRLLTFVLFLTSFFLPMTAQEDLSGYSYTVLGNYEYEGILY
                     SYGYVTGEFLVESDGYLQVKSEEETITVMFDSGMSEHAVFMAVLEKNIAVVYERYMVN
                     DETGIIFCGISIVTYSANGNVQGRLDYRDIPVCYGNRNFLLLVKWANEKVDIINSYLS
                     LCYEKDVAGTYENTFNYQYQGTAQVNGTYVESIDISTPGNYDIIIEDDIGIKEFNIVV
                     DPLVEGIENGKLYTDEVTIFAKGDLYLDDTTFLSGSTVSIAGIHELTIVGEGGYEKNY
                     LFSIAIRLENVYDGMETSEGIRIFANTTELVINGEIYEGGIISKAGSYVLVASGVGGM
                     EETVSFSILPALFGADNGAIYTDSVEIWTNCDFLMNGEMESEHCIVDEAGKYEVILMM
                     EGSEYSRLTFSIEKDKNTFGFLISPIFWTSVMAFIAVTGLVIIYKKK"
     gene            complement(8718..9908)
                     /locus_tag="PLP02_01590"
     CDS             complement(8718..9908)
                     /locus_tag="PLP02_01590"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPF42195.1"
                     /translation="MAKIMAVNAGSSSLKFQLLEMPEETLITSGIVERIGMKDPVFVI
                     KYKGERIEEKKDIPNHSVAVRLLLEKLIDLKIIKSFDEIEGVGHRILHGGSYLTESCI
                     ITEKEIGIIESIIDLGPLHLVPNLTGIRAFMEILPHVKNVGVFDTAFHQTMPEESFMY
                     AVPYEWYERYHVRKYGFHGTSYRYVTQRTAEILGKKPENTKLIIAHIGNGASLCAVKD
                     GKCVDTSMGLTPLEGIPMGTRTGNIDPAVVEYMMGQKNKSAKEITNMLNKQSGYLGVS
                     GVSSDSRDIVDAAEKGNARAQLAIDIQAKRIADYIASYYVYLEGADAIVFTAGIGENA
                     PTMRHDVVKRLKVLGVKIDPELNECHGVEKLISTPDSKIKVYVVPTNEEVMIARDTLA
                     LCKK"
     gene            complement(9913..10839)
                     /locus_tag="PLP02_01595"
     CDS             complement(9913..10839)
                     /locus_tag="PLP02_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPF42196.1"
                     /translation="MLDHNNIEKLFYAIDEIASLYSAKRQMNGIEGVATACNVIFAAD
                     QEDKEIRNIIESVKELKLGKEEIRKAFQLAVLKRLKQEGRSNSFITPDTIGVLVSYLV
                     ERLAVVPEEPTIFDPLVGTGNLLASVVNNLDRKTTAIGCDIDKECGNLAMALMNMLGE
                     KANIILADTLSLEGVKADIIVTDFPFSDESEHDYFPYKVLEHHYDNLINDGYVVSVIP
                     NDFFDITDTRDYRDSILAKYLAIGLIKLPDDLFKKLGKSILILRKKTDAKKKNENFLI
                     AEIPSFADQDEFKKTLAHINDWFDRYIDRKDK"
     gene            10973..11215
                     /gene="yidD"
                     /locus_tag="PLP02_01600"
     CDS             10973..11215
                     /gene="yidD"
                     /locus_tag="PLP02_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPF42197.1"
                     /translation="MRKLAIKLIRLYQKASVGKRPTCRYRPTCSSYAIQCYQNYNVFS
                     ATILTIWRILRCNPLSQGGYDPIPKVKKAERERIKG"
     gene            complement(11343..12080)
                     /locus_tag="PLP02_01605"
     CDS             complement(11343..12080)
                     /locus_tag="PLP02_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF42198.1"
                     /translation="MKAWAYAKRNTMEILRDPLNLAFGLGFPLVLIMLLTAIQANVPV
                     ELFELDQMTPGIAIFGLSFISLFSSVLIAKDRSSSFMMRMFSSPMTSGDFIAGYTLPL
                     IPMAIVQIGICFLAAMVLGLGATWNILLCIFVSVPAILFFIAIGLFCGSVFTDKQVGG
                     ICGALLTNLTGWLSGTWFDINLVGKGFKIVAEALPFVHAADAGRAALSGNYSEIMPDL
                     WWVIGYAVAMIAIAIVAFKKKMNSDNN"
     gene            complement(12077..12811)
                     /locus_tag="PLP02_01610"
     CDS             complement(12077..12811)
                     /locus_tag="PLP02_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42199.1"
                     /translation="MYAIETFDLTKKYKKVLAVDKLNLKVEKGELFSILGVNGAGKTT
                     TIKMLSCLTRPSSGEALVHGDSVISSPIKVKEKISISPQETAVAANLNVYENLCLIAG
                     VYGYDPIAAKRMADNAIQKYGMEEVAKKRAKTLSGGWQRRLGIAMALITEPEVLFLDE
                     PTLGLDVLARRELWKIIKSLKGKITIILTTHYMEEAEALSDRICVLAKGSVKAIGTPV
                     ELKEKTNAANIEDAFIAIVGEEGDGK"
     gene            complement(12813..13094)
                     /locus_tag="PLP02_01615"
     CDS             complement(12813..13094)
                     /locus_tag="PLP02_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPF42200.1"
                     /translation="MSEKCIGAVRVGPKGQVVIPKEIRDMFGIKPGDSLVILADKEKG
                     IAIERMNVFNKIADAIFAGKSQEIYPDVSEEDAMSFARGIKSLNGKDDK"
     gene            complement(13240..13323)
                     /locus_tag="PLP02_01620"
     tRNA            complement(13240..13323)
                     /locus_tag="PLP02_01620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13287..13289),aa:Leu,seq:tag)
     gene            complement(13330..13405)
                     /locus_tag="PLP02_01625"
     tRNA            complement(13330..13405)
                     /locus_tag="PLP02_01625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13370..13372),aa:Lys,seq:ttt)
     gene            complement(13413..13487)
                     /locus_tag="PLP02_01630"
     tRNA            complement(13413..13487)
                     /locus_tag="PLP02_01630"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13452..13454),aa:Gln,seq:ttg)
     gene            complement(13501..13577)
                     /locus_tag="PLP02_01635"
     tRNA            complement(13501..13577)
                     /locus_tag="PLP02_01635"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13541..13543),aa:His,seq:gtg)
     gene            complement(13701..14549)
                     /locus_tag="PLP02_01640"
     CDS             complement(13701..14549)
                     /locus_tag="PLP02_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPF42201.1"
                     /translation="MEKIGILIDSTSLTRAELKEYPFIKIVSLYVTLDGTSRKEEDIT
                     TEEMIEQLHKAKHITTSQPAPGEFLEKYEEFHNEGYTNVLVVTISDKLSGTYQSAMIA
                     KTMIDFEMDISIHAPQLASFGVANGVALLAEMVAEGASFEEVQKRYYTIFKNGAVMFT
                     LGNLMNLFRGGRLNRIQALLGTVLRIKPIVEMVEGKLQLTKKERTNLACFEYFMNKAD
                     EYCQKFKKVYFDIIHLNRPEWAEKIRLYISGKYPAAGIHMTDYVSPVFFAHLGDQGFG
                     LAVAAE"
     gene            complement(14685..16757)
                     /locus_tag="PLP02_01645"
     CDS             complement(14685..16757)
                     /locus_tag="PLP02_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPF42202.1"
                     /translation="MKEYSAKSLKSVILLGHLGSGKTSLVESLAYVTGRIEKKGEVEK
                     KTTISDYSPEEQAKQNSLSTSVVPVEYDDIKINVLDAPGADEMIGDVSYAFNAASGAI
                     LVLDATKGVEIGAEKMWLEIKKRNLPAIIFVNKMDKENVKFDSVMTAVKEKLGKQAMP
                     FCVPIGKSDEFSGFADIIDLKARLYDGKSVVDGEIYPDKMDKVQGYRTELVETVAGSN
                     EELLEKYFGGEELTLEEVKKGLHTTVLSGEVVPVIVGSATKDIGSVSLLGMIHEYFPS
                     MEEAESFKGSAPDSDDVISRKVSDTEPFSAYVFKTTIDPFMGAINIIQVRSGTVHKDQ
                     EVYVSSVQETRKIGNLFFLCGKTQIPVEVVHAGDICGIAKTDGVVTDSTLSDKKSPIV
                     YPKTYTPTPTMYIAIHPKNKADEDKLSNALQKLQLEDNTFEIRRNKETSQLLIGGQGI
                     IHLGYITDKMKNMFKVDIDTEEQQIVYRETIKATATAEGRYIKQSGGSGYYGVVVMRF
                     EPNPDKDYEFTEEVFGGSVPRNYFPAVDKGLQETLEHGTLAGFPVIGVKGILTDGKYH
                     PVDSNELAFKMAASIAFKEACKQAKPTILEPIDKIVVTVKDEYMGDVLGDVNKRRGRV
                     MGMEPAEAGYQKIVAEAPQAEITKYTIDLKAMTQGSGTFTREFVRYEEVPGNLIGKII
                     EEHDKKNN"
     gene            complement(16977..17579)
                     /locus_tag="PLP02_01650"
     CDS             complement(16977..17579)
                     /locus_tag="PLP02_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009006981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HPF42203.1"
                     /translation="MYWLYSNSGGKTGRKLSEMTKKELWCVFPIFLESHKTYWKSWFI
                     EEKAILENILPRDAVVRISHIGSTSIDGIHAKPIVDILVEIRLGKPMSQIKDLLVKNG
                     YRFMSDEDNRLSLNKGYTEQGFADRVFHIHVSYQGDNDEIYFRNYLTSHPDIAKEYEE
                     LKLKLWKQYEFNRNEYTEAKTDFVRKYTMIAKQENPGKND"
     gene            complement(17637..18023)
                     /locus_tag="PLP02_01655"
                     /pseudo
     CDS             complement(17637..18023)
                     /locus_tag="PLP02_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(18189..18263)
                     /locus_tag="PLP02_01660"
     tRNA            complement(18189..18263)
                     /locus_tag="PLP02_01660"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18228..18230),aa:Trp,seq:cca)
     gene            complement(18341..18955)
                     /locus_tag="PLP02_01665"
     CDS             complement(18341..18955)
                     /locus_tag="PLP02_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPF42204.1"
                     /translation="MAKNQVFPTKGNLLAFKKTDQLAKLGYDLIDRKRNILIREMMQL
                     IESVRQMRTEITDIFQTAYIALQEANITLGIVSDLAKSVPIDDGLSITYRSVMGVDIP
                     SLSYDKKPVRLNYGIASTNTKFDYAYQNFIKVRDLVIKLAEIDNSAYRLAFAIRKAQK
                     RANALKNIVIPELQENIKFIADSLEEKEREEFSRLKMIKNEKNR"
     gene            complement(18958..20403)
                     /locus_tag="PLP02_01670"
     CDS             complement(18958..20403)
                     /locus_tag="PLP02_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPF42205.1"
                     /translation="MNLQYIGLDEINGPLIFMDGVENIGYEEMVEIRLTSGEIRYGRV
                     VLISGKKVAIQVFEGTFGLSLENTRTKFLGHPMEIALSKGILGRVFNGSGRPMDGLGP
                     IFADENRDINGLPLNPVSRVYPRNYIHTGISSIDALTTLIRGQKLPIFSGSGLPHDEL
                     AVQIVKQAQIAEGEGKNFCIVFAALGVKNDVADYFRRSFEEAGVFDRVVMFLNLANDP
                     IIERLLTPRCALTAAEYLAFKHDMHVLVISTDITSYCEALREFSSSKEEIPGRKGYPG
                     YLYSDLASIYERAGIIKDAKGSVTQIPILTMPNDDITHPIPDLTGYITEGQIVLDREM
                     NHLGIYPPIGVLPSLSRLMKDGIGAGYTREDHQTLMNQLFASYARVQDARNLSAVIGE
                     DELNETDKMYIEFGRLFESRFLTQRHDENRSIEETLDLGWALLSTLPKEELDRTEDEY
                     LDKYYNPAAAKKEFKIRDKSIIRELDRGSES"
     gene            complement(20415..22160)
                     /locus_tag="PLP02_01675"
     CDS             complement(20415..22160)
                     /locus_tag="PLP02_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPF42206.1"
                     /translation="MNKIFSINGPVVTVRDTTDFSMMEMVFVGNKKLLGEVIKITSVA
                     TTIQVYESTTSLRVGEPVIGTGSPVSVTLGPGIMQNIFDGIGRPLKTISEKYGAFIAS
                     GFQMSSLDEEKEWEVKLLCKKGDVLAFDDIYATVSETELVLHKLMVPKGIGGTVVEVK
                     RNGKYKVNDTIVKIKTEDSSTVELSLVQRWPIKTPRPVRERLQITTPLITGQRVIDTL
                     FPIAKGGTAAVPGGFGTGKTMMQHQLAKWCDADIIVYIGCGERGNEMTQVLEEFGELL
                     DPRSGKPLTNRTVFIANTSNMPVAAREASIYTGLTFAEYYRDMGYNVAVMADSTSRWA
                     EALREISGRLEEMPAEEGFPSYLPSRISQFYERAGYMSNINGTLGSVSIIGAVSPQGA
                     DFSEPVTQNTKRFVRCFWALDKQLAYARHYEAINWNISYSEYVPDLAKWYNKNVGPRF
                     IDNRGKIMKLLTEENKLMEIVKLIGSDVLPDDQRLILEVSRVIRVGYLQQNAFHKDDV
                     FVPLPKQLRMMDVILYLYQKCVEILKEGKAFSLIQKTKIFEKIVKMKYDVPNDKLEMF
                     DDYFPMIDDVVKSVK"
     gene            complement(22192..22794)
                     /locus_tag="PLP02_01680"
     CDS             complement(22192..22794)
                     /locus_tag="PLP02_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPF42207.1"
                     /translation="MDYYTNDQLYKYFEDEIIMNAGKQMKALKAEIDDLKKKALEKIK
                     SDLSESSQTELAAKIRDMETDHSQELNRISYDYSHKLMQKRQEYMDQIFAVAKTKLQD
                     FANGPEYTKMMKSNLAGIYTKLSGGKIDFRIRKGDENLAGIIKDTCKEQNTVSEDPAI
                     KIGGFVALSEKHALQIDETIDSALESRKEWFYSRASLLFE"
     gene            complement(22806..23114)
                     /locus_tag="PLP02_01685"
     CDS             complement(22806..23114)
                     /locus_tag="PLP02_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPF42208.1"
                     /translation="MKFFVISDNVDTLVGMRLVGIEGVVVHLRQEVLVELEKAMQDKE
                     NCIVLITTKLVELCPEVISELKLTRDIPLIVEIPDRHGGTNIGETIDKYVSEAIGIKI
                     "
     gene            complement(23133..23570)
                     /locus_tag="PLP02_01690"
     CDS             complement(23133..23570)
                     /locus_tag="PLP02_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPF42209.1"
                     /translation="MLNGFEWLLPLALLILISLPLVNVFRGKVSFISAKRRVLGQVFG
                     FFGLLILTAVFEIKGVWVAAAGETTAFDGSIAQGLGFLSAALAAGLSSLGAGIAVAAG
                     TPAAIGALSENEKTFGKSMIFVALGEGIAIYGLLISILIINKL"
     gene            complement(23613..25532)
                     /locus_tag="PLP02_01695"
     CDS             complement(23613..25532)
                     /locus_tag="PLP02_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPF42210.1"
                     /translation="MAIAKLKLINITSNLSNLEPVLAKFVSLHCFSPVLSSKFVERVH
                     GLRTISEENPTETILEELSEIEKEFDIEISADGTEITEYSPDKMKNYISTTHKRLKEL
                     IEKKKELQEQTEKYENALVQVRNIESMDISLDDLFSCRYIFARVGRLPKSSIEKLDYY
                     RSRPFIFNSFGTDKEYSWCMYFTSPEYEREVDNIFSSLFFERIHIPEFVHGTPESAET
                     QLTREMNNFCKESSELQTEITSFLQESKHELVAIKNELLFLAKINEAKKYVVNLGEKF
                     SITGFAEATDVERIKERFANIKDVEVTVRPAGADKRLVPPTKLKNNWFTRPFSTFVNM
                     YGTPSYADIDPTPFFAVTYSLLFGIMFGDVGQGLVLVLLGILLARKKKSSLGQIAIRI
                     GMFSTFFGFIYGSVFGNETLLLPLYKLFGMQNKPVDVLSNDFTSTLLTLAVIIGAALI
                     ITSILINIMTRRKKKEYAEMIFSHNGIAGLILYSYVAVSLLAKFFYGVSFFTLWALLL
                     FIGIPVLMIILKQPFVRKFAGQKFFTGRIGGFIADTFFDLYEIALSYLTNTVSFLRVG
                     GFVLSHAGMMLVVYTLMSSVGNFVGEALVFLFGNMIVMALEGLVVGIQVLRLEYYEMF
                     SRYYEGDGVAFTIFE"
     gene            complement(25589..26566)
                     /locus_tag="PLP02_01700"
     CDS             complement(25589..26566)
                     /locus_tag="PLP02_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42211.1"
                     /translation="MALSKIRLAVISTNKTNADSALASFANIHCFAPVDAIKFVDVVH
                     GLSALSDENPCPAYLGEIAEIEKKFSFLAEPKIVKTSTMGIIKAKKAIDKIKTELSDL
                     VNKRNEKKKELDKYEEAYKQVKTLAGLNTPLEDIFSCDYVYARFGKLPNDSVEKLEFY
                     QDKPFIFKSFAEDKNYSWCMYFTSKEYEREVDNIFSSLFFERFYIPDFVHGTPKQAAM
                     TLADKKGKIAKELEEIETKITNALATASEELSEVKGELQFLNRVNAAKKYVVVLGSKI
                     SISGFVRSKDVHRITEMIKSNPKLGLEIMPADADARLIPPKILKKENFY"
     gene            complement(26590..27627)
                     /locus_tag="PLP02_01705"
     CDS             complement(26590..27627)
                     /locus_tag="PLP02_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPF42212.1"
                     /translation="MGSFAANAMVAKTKAIYGTLLKAEDFEELSKKKSVGEIAAYLKN
                     HPDYQETLADIQESSIHRGQLEELIKKNNFLHTLKVAKFVELKDKSYYHLNIIHREID
                     LILATIRMLISENIDIAIAEFPTYFIRHASFDITAFSRVRDYGMLLEAVKGTKYQAIL
                     EPYFETDRNLIKYSAIEQKLNSHYYDCVFERIAENYSGSVRKELEIMFMTRIELANII
                     LAYRLKKFYDASPDEIENLMDFKHIRTSKKRAREIIENTDADGILSFLRDSEYARYAD
                     DNDFVYVEYFGDKIRYHLAKKDLYYSNSAPIVFSAFLIMEEIERQNLFHIIEGIRYGL
                     PESEIRKMLIY"
     gene            complement(27651..27965)
                     /locus_tag="PLP02_01710"
     CDS             complement(27651..27965)
                     /locus_tag="PLP02_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42213.1"
                     /translation="MEKLIKDIVELDKEYRLKVDALRKEKDKIDAYIRDEKTKMEAEY
                     KGQTEKALTDKKDKLDKEMDKRKREFEISYQESIAKMEGAFKAHFQEWTDQVYNACLK
                     EE"
     gene            complement(28093..28302)
                     /locus_tag="PLP02_01715"
     CDS             complement(28093..28302)
                     /locus_tag="PLP02_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPF42214.1"
                     /translation="MKKAIIEGICCNGCARDVQHILEGIYGVTDVSVSAEDGVATFKG
                     YVSKKVIAATLEEEGYHLVELISEE"
     gene            complement(28351..29928)
                     /locus_tag="PLP02_01720"
     CDS             complement(28351..29928)
                     /locus_tag="PLP02_01720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPF42215.1"
                     /translation="MNYIALLVGFTFAMALISLFIGIYILVKLKSAHGFWKVSVLFFL
                     EFIYAFGYAMELASNDIVIKVIFNHIQYFAIPFIVVVWIYVAKKFRDAQYSTKLNHYI
                     ILLIVPFITFLSVQISYFTGFDLYYSEAELTQFSFFGIETVTVLSLTKGPLYFLNISY
                     NASLTAYVAYIYINNYMQTHGTRKKQSLIMAMCSIIGLGSSFVAYFTKVTTGIDWSLY
                     ILVFISYIIIYAIFRYELFDLQPEAYQHAFEINTLPLMILNEQRELVTWNKAATEFME
                     KENPPYLHMPLEQMFGSDREMLAAIIDMQPFSFTKGIKHYVLETISLISPISRSLKGY
                     MLKFNETTTFVDKIEKLDYQASHDELTKILNRRAFHEKAREYLAGMNKRHAEFSIIYM
                     DIDDFKSINDTYSHMAGDYVLKTFAETIFELLPDDSLFARYGGEEFVVLLKNISREDA
                     IAMAQGIRIAAMKKEYSFEGKKIDLKVSLGLYNHSGSKPIDIYDLIFLADKALYRSKN
                     TGKNKLTVYEPDENEMV"
     gene            complement(30030..31190)
                     /locus_tag="PLP02_01725"
     CDS             complement(30030..31190)
                     /locus_tag="PLP02_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF42216.1"
                     /translation="MKMSETFKFKLFYFIRYFGDAFFYPFMSIYFVSKGISDSDLGLI
                     FAITPITTILVNPFWNYIVKDIKTSRLILKIMTVIEGAMIITMTQISGFEMYALIVSI
                     IAFFCSPFINIQDGFTATFCNNEKIEYSSIRIYASIAYVVASAIAGSAILWFGYESVF
                     IISGTFFILTSLIVFWLKPFKKDAEVSEDTKRDIKSLLKNKNFYKYLIYYTITIGAVR
                     IGDSFFGVYITDLGLESQWYGLLYAAFVFVEVITLRIMITKGQSMGERKLLVLANFLF
                     VARFIFYGFDLPLSVIIPVTLLRGASWGILIYANIHYIIKIVKVENVTTAILIITLFF
                     SIFTAIGNYGFGLLADANGYSMPYQIVAATVAFGFVFFLAFSPKIPEETDKQ"
     gene            31590..33707
                     /locus_tag="PLP02_01730"
     CDS             31590..33707
                     /locus_tag="PLP02_01730"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPF42217.1"
                     /translation="MIKQIVKRDQSVVPFRREKIVLAIFKAASSVGGSDFAKSEELAA
                     EVVGMAEAKYPNGIADVEGIQDLVEKVLIENGHAKTAKAFILYREKRRASRESNALIG
                     ATIDMFTEYLNDRDWQINENANTQKSINGLNNYVREEFTKNYWLHEIYPEDIRNSHIQ
                     GDIHIHDLGFFGPYCAGWDLRQIITNGFGGVSNKVESSPPKHLRSLLGQIINSTFTTQ
                     GETAGAQAWSSFDTYLAPYVRYDNLDYKQVRQAIQEFIFNLNVPTRVGFQCPFSNLTF
                     DIIPPKTMRDQNAIYAGQLMPETYGDFQAEMDTINLAFCDVMMEGDAKGRVFTFPIPT
                     INVTKDFNWNSPVVEKYMEITAKYGIPYFSNYINSDLSPEDALSMCCRLRLNTSELRK
                     RGGGLFGSNPLTGSIGVVTVNLPRLAYLSRSESEFFTRLWETMNIAKKSLEIKRNIIE
                     EQTDNGLYPYSRNYLKDVKLKSGSYWFNHFNTIGLIGMNEAAMNLYGKEVDLTTEKGQ
                     DFAFRTIEYMRDVIKDIQEETGHYYNLEATPAEGTSYRLAKHDKERFPDIITAGKDTP
                     YYTNSSQLPVGFTDDIFETLDLQDKLQSMYTGGTVLHLYLGERVKDTETTKNLIRKVF
                     ENYKLPYISLTPTFSVCNDHGYISGEHFNCPTCGKPAEVYTRVVGYLRPVQNFNKGKR
                     AEYADRVKYVIRNEKTAEEDRKA"
     gene            33704..34396
                     /locus_tag="PLP02_01735"
     CDS             33704..34396
                     /locus_tag="PLP02_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPF42218.1"
                     /translation="MIFAGIAKTSLVDFPGKIATVLFTPGCDYDCFYCHNRSLIEDFQ
                     DIVPNEEIEEFLTKRVGLVDGVVITGGEPTLHSDLISYMIKLKGMGYLTKLDTNGSHP
                     EIVEKCIEAKAVDYYAVDYKAPKDRYKEICRGESDADVVLQTISLLLSANQDFEVRTT
                     VIPQLTLEDLITMAEELPLVPKYTLNPYKKPLHFREEDRALIEAQPYSEKDIAAFAET
                     LKLYQPNTVLVF"
     gene            complement(34462..34546)
                     /locus_tag="PLP02_01740"
     tRNA            complement(34462..34546)
                     /locus_tag="PLP02_01740"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34510..34512),aa:Tyr,seq:gta)
     gene            34691..35053
                     /gene="mgsA"
                     /locus_tag="PLP02_01745"
     CDS             34691..35053
                     /gene="mgsA"
                     /locus_tag="PLP02_01745"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPF42219.1"
                     /translation="MKIALIAHDKMKQEIIDFVTKYRDVLSKHELFATGTTGHRIIEA
                     TGLNIKCFRSGPLGGDQEVGARIANGEIDLIFFFRDPLTAQPHEPDVSALLRLCDVYR
                     VPLATNRSTAELIAHAIK"
     gene            complement(35093..36208)
                     /locus_tag="PLP02_01750"
     CDS             complement(35093..36208)
                     /locus_tag="PLP02_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPF42220.1"
                     /translation="MKSLFYPDYKNTILNVSNSLLKYYGCETFAPSLPLLDNILANKE
                     FRNIALVLVDAMGESTLQNHSEQAKNIASDKVATISSVFPPTTVAATTSVLTGKPPIS
                     TGWIGWMQYVRDIDRNVVFFKNKDYYDESAKIDIPVANTYVPVTEIYDIFKAKNPDLT
                     TKEIFPAFREPEHDTFAKQLETLNTEFEKPGRHFAYVYWDQLDSVMHEFGPDSQEAAD
                     TLDAVDKGYSAIKEKLPSDSIVIMIADHGQIAVKPIKLYEYEKIWSLLEKKPSIESRA
                     VTFWVKEGKKDDFEREFLSNFRNEYILYKTEEILKMNLFGYTSEHPRFREFLGDYMAI
                     AISDMYFVFFQTDFFMKGQHAGLTSEEMLVPLVVFSK"
     gene            complement(36205..37623)
                     /locus_tag="PLP02_01755"
     CDS             complement(36205..37623)
                     /locus_tag="PLP02_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPF42221.1"
                     /translation="MKPEIEKKTHKILYGNPIWKGLLSLSVPVFLVNILKTLHDVVDG
                     IFLGQVPDVDGFSVSTSMQSAVALSWPVYFVFLSFGMGLSVAGNSLIAQYIGNKDKAN
                     AEKYALNTVVLSVILGAIFTIFVYFMTPLIFRFMGAQGSDYSYAVTYMRIRSFEFPVL
                     FLAFAFQAVRQATGDTVTPVIISAFSIVVNLVLTPIMILVWNMGIVGAGLSTLIAQWL
                     ALPFILLLFVYARKGIQVHWRIHEVKRFIINDIIKIAVPASTGQSIQALGFVIMNSLI
                     YSFGTEVSAAFYIGNRINSLVMFPASAVSSIVAIYIAQNVGAGNIPRAKKSFHSGMII
                     SILMMSVGAMLIIPFRDFFVSLFNHDPETIALAAQYTFYLNIGLPLMAIFQTYLSTFQ
                     GSGETKFSFLMAVIRLWVIRLPLILVVINFTDLGPSGIWYSMLISNILIVFVGMFLYS
                     KVRFLPKIRTSRIINDQEEEIA"
     gene            complement(37754..38434)
                     /locus_tag="PLP02_01760"
     CDS             complement(37754..38434)
                     /locus_tag="PLP02_01760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF42222.1"
                     /translation="MDVRKTNRDLIAFWDKNFEKLTPEKVEITDIPEDVLSGYFKQLG
                     NQCENILDVGMGSGYGVFTAALAGTKVKNIIGIDPSENAVKYVNGVVNLSGLHNIECF
                     LGDHSLLSDWRAESFDGIICSNVLDVVPSETSDKIASEIVRLLKPGGLFLLKLNFYLT
                     DELIKKLNMVEIEENGYSINGIMRGLNRTIEEWKNIFDGFSVLEESEYERIPNGPKDR
                     IVLFRKGK"
     gene            complement(38575..38650)
                     /locus_tag="PLP02_01765"
     tRNA            complement(38575..38650)
                     /locus_tag="PLP02_01765"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38615..38617),aa:Thr,seq:tgt)
     gene            complement(38652..38727)
                     /locus_tag="PLP02_01770"
     tRNA            complement(38652..38727)
                     /locus_tag="PLP02_01770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38692..38694),aa:Val,seq:tac)
     gene            complement(38850..40127)
                     /locus_tag="PLP02_01775"
     CDS             complement(38850..40127)
                     /locus_tag="PLP02_01775"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HPF42223.1"
                     /translation="METKPEDILNDREKRANMIALFLRDRDVVVTIRANIPGPDKRLP
                     GAYVLIRHFSKLFYPEQQNNSLFVDGADGPTIFRFIDNVSAKDLKKEAEWFENTEEVG
                     RLLDIDVHTKEGTLHRDILRKCVICDDYAVNCVRAKKHDQNEVLRIYQEIFDDKFMTL
                     LQDVIDKAIMAELNLDPKFGLVTPTSSGSHRDMDYAKMLKAKDAIIPYLTKMFIVALR
                     VDVEKNIFPEIRKIGQEAEKAMFRATNGVNAYKGLIFSLGIIIASTAMVLHGKGQFER
                     IFIRAEELSSGLVKELETGHDTFGKIAYQKYGIAGARKEAEMGFPLVSEALKTLNDIY
                     DPLVLTKTLVYLIGTCEDTVFLKRSVTIEDYHKYQNMFKDLTDFSEGNIKKMTDYCIE
                     HNLSFGGSADLLVVAAFLEEFRKTFHLDIPTLS"
     gene            complement(40111..41121)
                     /locus_tag="PLP02_01780"
     CDS             complement(40111..41121)
                     /locus_tag="PLP02_01780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42224.1"
                     /translation="MIVKKALIREEFNRLRIFLDAFGLKYDDDINESFYIEDQGEIIA
                     TGSRSKNLIKAIAVSKEHRGENLASLLLTELLASMRAEKIFYYQVYTKAEYLSIFESM
                     GFSFIEKTDKVAVLEGGDGNIETTINRMKKRIEERFKRSLDKADIGAIVVNCNPMTKG
                     HFQLIIDSAANHEIFLVLVVEEEKSFFTYPERLLLINKALAGYENIMVIPSTGYVVSS
                     LTFPSYFLKSIDEAEKEHAKLDAMIFRDYFIRILNIRKRYVGGETEPVMVTYNNLLKR
                     YLGDTLVRRERFQQDNEPISASRVRKLILEGKPREASMLVPDAARNTFMEIVEKKYGN
                     KA"
     gene            complement(41129..42637)
                     /locus_tag="PLP02_01785"
     CDS             complement(41129..42637)
                     /locus_tag="PLP02_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPF42225.1"
                     /translation="MKNSLGREIPEGNKPFISAANFSERIYHEAKEKKPAENVVFLNS
                     LEAAFDVLSIMPGMRLSFHHHLRSGDHVINEVAKLIEKHDLRDISLAPSSIFPEYMLA
                     PLIRSGRIRDIHTSYLNGPVAIAIQEGFLKGHLVMQTHGGRARAIEEGELPIDVAFLS
                     CPTVDSSGNGSGAVGKSACGALGYGISDLYHAKKVVLVTDNIVEKLDEYQFDHRYVDY
                     VVKVPSIGDPEGIVSGTTKITRDPIGLKIANITALLLDKLGLLETGTSLQTGAGGTSL
                     AVMEQIEKKMRSKKATGSFLSGGITGYHVEMLESGIFKNIYDVQCFDLEAVRSYRENS
                     GHIGISASQYANPFYKDAIVDKLDAVILGATEIDLDFNVNVTSDSFGNIIGGSGGHSD
                     TAHGAKTTIITSALTKARIPIIKEHVTTVTTPGEDVDILVTERGIAINPLRKDILEKL
                     NDSGLPLIDIRELMELSHKICGVPADIAKPAKTIGYVEYRDGTVIDSLYKTR"
     gene            complement(42627..>43121)
                     /locus_tag="PLP02_01790"
     CDS             complement(42627..>43121)
                     /locus_tag="PLP02_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPF42226.1"
                     /translation="ASLVEAKNIAFCPRVEAMMLGAEDFTADMGITRTKKGDELAYPR
                     AKLAIICKAYDILAIDTPFTDVNDDLGLQDDALTAMNLGFTGKAAIHPRQVGIINNVF
                     SPTEEKITWAKRVMEAKKDADKKGFGVFSLDGKMIDKPVIARALAIREQARACKAWDD
                     NDEE"
BASE COUNT        11691 a        10111 c         8566 g        12753 t
ORIGIN      
        1 ccccgctctg agtgttacca ttccgttttt gtattcctcg ggatctccga taccgtaaat
       61 acatgatacg gaggccacca cgatgacatc ttttctttcc aaaagcgaag atgtggcagc
      121 gtgtcgcatc tcatcgatct catcattgat tttcgcgtct ttttcaatat aaagatctgc
      181 cccgggaaca taagcttccg gctgatagta gtcataatag gatatgaaat attcgaccct
      241 gttttcagga aacagactct tgaattccga ataaagctgc ccagctaagg ttttgttgtg
      301 ggcaaggacc aaaacaggtt tctgaacctg ttcgatgacc ttactaatgg tataggtttt
      361 gccggtaccg gtcgcaccaa gcaatacctg ttctttcatt tcgcttctaa gattttccgt
      421 caatttccta atggcttccg gctgatcgcc ggttggttca taggaagata cgaccttgaa
      481 tttattcatg ccatcatcac cgtttatagt atatcatatt aggataagca tatggcataa
      541 atccgttttt ctgttaaaat ggtatcggtg atgaaaatga aaaccctgtt ggtcgcaatt
      601 gatgccaaat atatccataa gaatgtcgcg gtcaggcttt tgaaagcaaa ctgcgatttt
      661 cctgtcgacc tcttagaaac aaatatcaag gaaaacgtcg acgatatttt cgataagatc
      721 gtctctttct ctccggatat cataggcttc agcgtatata tatggaatat tactgtcatc
      781 accaaactga ccgaaagaat caaatcatca acaaaagcca tcgtcatctg gggcggcccg
      841 gaagtgtgtt acgattatga atactttctt tccaggtttt ccgtggattt cgttgcgatt
      901 ggagaaggcg aactgacatt caatcagttt ctgcatgcca taaatgaaaa cagcggcttc
      961 gatcacatat ccggcctagc atttaaaaag gaaaatattg tctttgcgac aaagcctgcc
     1021 gaaacaatag atttgacaga actcaagtcg ccttaccgct tccaagatga tatcgccgaa
     1081 ctgccttatc agattcaata tctggaatcc tcgcgtggat gtcctttcca ctgttcctat
     1141 tgtctcgctt cacttgaaaa caaagtacgt ttctttccgc ttgacaaagt caaagatgac
     1201 ttgttatttc tgattagaaa cggcgccaga acgttcaaat tccttgacag gaccttcaat
     1261 attgataata agcgcgcaat agaactggct gactttatca tcaaaaatca tctgcccggt
     1321 aattcctttc aattcgaaat aactggggaa atcctatcgg acgaattcat tgatttcatc
     1381 aatttaaacg ctccgaaaca tcttttccgg tttgaaatcg gtatccagtc gacaaacgaa
     1441 agcagcaacc tggcagttag tagacgccaa gacaacgacc gattgttctt tgtcatccgc
     1501 aaaatcaggc aagccggcgt catcgatctt catcttgatt taattgccgg gcttccttat
     1561 gaagacctcg acagtttttc gaaaacattt gatgccgcct tttccttgcg agccaaggaa
     1621 ctccagttgg gattcctcaa gttattgcgc ggaactccaa tcagaaaaaa cgctgatatg
     1681 cttggctata gttttaatca agatccacca tatgaaataa cggaaagccg gtggatatca
     1741 aaaaatgaca tgaagaaaat ccgccaggtc gaacaggcat tgaatatttt ctggaacaaa
     1801 tttttcatga caaaaaccat cgaaaaaata gccgatgttg aattgtcaat atttcatttt
     1861 ttcacaaatc tgtttttata ttgcaaaaga gttggatttg ttttccaccg gtatcaattg
     1921 tcggatgttt tccaggtatt gaatgattat ttatgcaaaa ccaatccttc ccttcatgat
     1981 ttagtattgc cgctgatgaa acatgactac ctgtcctatc acaaaatcaa accaaaaatc
     2041 tggtggaagg aagacaaact ggaaagacgc gaagttttcc gaactttctt agaaaagaat
     2101 catgactata aattggacac actatacaaa tacggaatac ttgcaaccta tggcgatctt
     2161 tttcttttgg ctatttacat gcccgatcat accagcatcc ttcttttcgg tcatgacggc
     2221 gtcattcttt cggatttgtc agaaaccgca ctttgatata taattaattt tgcttggaac
     2281 tatttcgcaa aggagtattc tcaaatgcca caggaaaaag ccttttggtg ttttgcccaa
     2341 tgaaaacagc gataatatat cttttcaccg gtaccggaaa cactaggatc gccggagatc
     2401 tgattgccgg acaattagcg aaacacaata ccagaacaac tatctatgaa ctcaaacaac
     2461 cgtttgaaaa tattcccgat cccaatcagt tcgatatggt tggattcgga taccctgtcc
     2521 acgctttcaa cactcctcgc ttctttttag actttgtgaa aaaacttcca gacactaaca
     2581 aaaaaaccgc ttttgtcttc aaaacgtcgg gcgaaccttt tgcattgaac aacgcttcga
     2641 gccgtccttt gatacgtctg ttggaaaaga agggcttcgt tccggtttct gatcgccata
     2701 tgctgatgcc ttacaatatt attttccgtt accctgacgc catggtaaag caaatgtatc
     2761 ttcatacagg aaaaatggct atgctatcgg taaacgacat tttaaaagga aaccacaaga
     2821 aattccgttt cagtcccttg acggtcatca gcctttatgt ttttcgtcta cagtggctgg
     2881 gtgccaagat aaacggtccc tggatccatg tggataaaaa tttatgtgcc ggatgcagtc
     2941 tatgcgttaa aatgtgtcca gccgccaata tcaccatgga aaacggattt ccgaaattct
     3001 caagccattg cacgatgtgc atgcgctgtg cgtttttctg tcccagcgac gcagtgcgtc
     3061 ccggattttt aaacggatgg cgcgtaaacg ggccctatca atttgaaaat atcgtaaaag
     3121 acgattccat accaacctca ttcgtccaaa aagacaccaa aggatatttc cgcctattca
     3181 aaaattatta cgagcggaca aacaaggaaa tcaaagaact ggaaaaatcc aaacaaaaag
     3241 cctgctaatt aatttggcag gcttttttat cagttcagtt tagacagata accgtacaaa
     3301 tcactcggct ccagtttcat gctcaaggct tcatggatgt cgcaatgtcc aacattcatg
     3361 ctacgggcgt atacggctct ccccccatat ccttccttga ggatatcgac ggcgtaagat
     3421 cccattttgc tggcaaggat ccggtcatat gaacttggcg tcccgcctct ttgtatatag
     3481 ccgagaacac aggccctcgc ttcgtattcg gatatctcgt cgatttctct ggcgagttct
     3541 ctgacatcgg tgatgttttc cgccaccacg atgacaagat gacgtttgcc gatttctctc
     3601 gcatggtgga cctgcttgaa aatctcttcc ttgtcaaatc cggtatcacg cgtaatcacg
     3661 atttccgctc cgcaggcaat gccagaataa atggcgagat caccgcaatt tcttcccatt
     3721 acttcgacca cagtgcatct ttggtgggaa aatgatgtgt cacgaagttt gtctatggca
     3781 tctatagccg tatcaagcgc tgtgaaaaac ccgatcgtgt attcggttga cgctatatca
     3841 ttgtcaatag tcccgggaag gccgatgcat ggcattccca tttcggtaag agccatggct
     3901 ccgcgatatg ttccgtctcc accgatcgtc actagagcgt cgattccgtg actgcgaaga
     3961 ttgtcgatgc ctttttgtct tacttccttt tcgacaaatt ccggaagccg cgacgatccg
     4021 agaatggttc cgccccggtt gattatctgt ccgacatcgt ttctttccag ttttcttatc
     4081 ttattttcca ccagaccttt gtagccgtca aacactccat aagcttccat cttgtaggaa
     4141 atggccgtcc ttacgacggc ccgaattgcc gcgttcattc caggggcgtc gccaccggat
     4201 gtcaatacgc cgattttcat cgtttattcc tcctttatcg gtttcatgga ttcgatcacc
     4261 atttgttgta cattgttacg aagttctatt tttcctctga caatgtagat tccgcctttt
     4321 tccaaggtgt ttttgtaatt tcggtaatcg cggggaaaca gtatggcttc cagagatacg
     4381 aacatgtctt cgaattcgag aaaggccatc tcttcctgat ttttcgtagt aattactttt
     4441 attttccgca aaagggccgc aaaggtcgtt tcttgttttt cccgaagcgt tgcttcggac
     4501 aaaagactta ttccctccct ccggaaaccg tcgcggtatt tggccaatgg atggaaactc
     4561 aaattgaatc cgatcagttc tttttccatg gatgccaaat aatcgcggtc atattccgga
     4621 tattcgacat agacaaatcc gaggttttcc aaactttggt tcatgctgcc gagcatcatt
     4681 atctgtttaa ggttcatcat catcgtctgt ttggtaagtc cgaaatcatc gaaaacgcca
     4741 gccataatca tcgattcgat tactctgaca ttgatatcct ttgcacgaat aacaaaatcg
     4801 gcaaagcttt tatattcccc ttcttcctgc aattgatcca aacggtcggc aacgaccgat
     4861 ccgatattcc gaattcctaa atacggataa cgcattccct ctttttccat ttggtagtat
     4921 ttgcgggatt tgttgattcg tggaggcagt atctttattc ccaatttccg gcattcctgg
     4981 atgtattcca aagtagctcc ctgcgatccg gcagcagaat ccatcaggac ggacatgaaa
     5041 tagcccggat aattggcttt gagatacgcc atccagtatg ccactagact ataagcgacg
     5101 gaatgactct tgttgaaacc gtaattggca aattttacaa tgtaatcgta gatccgatta
     5161 ctgacggttt cgtcccggtt catccgccgg cacttgtcta gaaattttgt tcgttcggca
     5221 accaatgtct cttctttttt cttgctgaca gctcgtctca aaacgtctgc ttcgccaagg
     5281 gaatatcctg catatacatg ggcgatctgc atgatctgtt cctgatacac tatgattcct
     5341 ttggtggatt tcaggattgg ttctagttcc ggatcgatgt aatctatctt ctcctgattg
     5401 ttcaaacggc gaagataact aggaatgttt tccatcggac cgggacggaa aagtgcaata
     5461 catgtcgaga tatcctcaaa ttcatgaata ttcattttcc cgagtagatt catcataccc
     5521 cgtgattcca actgaaaaat tccgatggtc cgcacttcgc gtaaaagcgc gaatgtcttg
     5581 acatcgtcta acgggagttt gtatatatcg attgtctttc caatgttttc cttgactgct
     5641 tccagcaccc ggcggatgat agtaagattt ctgattccga gaaaatctat ctttaacaat
     5701 cccaattttt ccagatcgat cgcttcgtat tgcgtctggt acaaatcaag caacccgggt
     5761 tgcaccggaa catgatccgt tatcggcgct tcggaaataa taataccggc agcatgggta
     5821 gaaatatgtt tcgggagatc ggcgattttc tcagcgatgt caaacagcat ctttatttcc
     5881 ggggttttca tgagataatc gtacttttcc gaattgtcat gtttgaacgt aacaattgaa
     5941 ttgtcggttt ccgagatata tttggttact tcatcaatga tcactggttt cgttccaagc
     6001 actcttccag cttctctcag agcgcttttc ccttggaaag tgccgaaagc gacgatattg
     6061 ctcacatggt cctttccata tttttccgat acatagcgta tgacttcgtc tctcttgtca
     6121 tcgggaaaat ccagatcgat atccggcata gtgattcgtt ccggattaag gaagcgttca
     6181 aaataaagat cgaaatccaa cggatcgaca tctactattc ccaaaacata ggcaaccaaa
     6241 gatccggccg aagaaccacg tcccgggcca accaagatgc ctgccttttt ggcataaaga
     6301 acgatatccc agacaatgag aaaataatcc tcgtagccca tcccttcaat gattttcaat
     6361 tcatgggcga gtctttcccg ataaatgtct ttgtttttcg tttttgtttt cttcgattcc
     6421 agacgttttt ccaatccttt ttcagccaac gcacgaagat attttgcagc cttgtgtccg
     6481 tcaggtactg gatattttgg caaatacaaa tgtccatatt caaattcaag atttatttcg
     6541 gaaatcataa tgtttgtatt attgatcgat ttgcgataat ccccatacat tttgtcgagt
     6601 ctggcgggag acttgaaatc ataaacagct tccgcttcag tcaacaaatc cgattcttcc
     6661 aaagagcttt ctttgaggat tgccttgaga actctcgatg cgacgatatc ctctttgctc
     6721 atgtaaagca ctttgttcat tatgacagtt ttatatagat tcaccagttc ggaagccact
     6781 tgcgtttccg tttcgaaatc attaagatcc aagccgagat ataattcccg acaattctct
     6841 tcgattaatc tgatgatctg attcgcaaac gaaaaatcgc ctccggcgac agctcggtga
     6901 acttccccgg cgtcagccat gatgacgcag atcactccgt tgctattttc tttcagatta
     6961 ccaagttgaa catggtcttt gtcgttagac agttgtgaac tgattttaac cagatttctg
     7021 tatccaatat cgtttttagc caataacaaa agcggaagat cgcgaaagat ttccgctgtg
     7081 gcttctacat acattccgat taccggcttg atgccggcgt ttttgcaggc ctgatggaat
     7141 ttcaaagcac cgtaaaggcg tccccgatcg gcgattccga gcgtgcggaa tccttgcttt
     7201 tttgcatttt ctaccaaagc atcgatgccg ataaggcttc cgttgaaaga gtatccggtc
     7261 tgaatataca gatcaaaatc tatcaagctt atcaccggtt tgattataac atatcgcaaa
     7321 acataattaa atgcgctatt tctttttgta gataatcacc aatccggtta cggcaataaa
     7381 agccatgacc gatgtccaaa aaatcggact tataagaaat ccaaaagtgt tcttatcttt
     7441 ttcgatggaa aaagtcaatc ttgaatactc gctgccttcc atcatcaata tcacttcata
     7501 tttaccggct tcgtccacta tacaatgttc actttccatc tcgccgttca ttaaaaaatc
     7561 gcagttggtc catatttcca ccgagtctgt gtaaatcgcg ccgttgtctg ctccaaagag
     7621 cgccggtaat atggaaaagg atactgtttc ttccatgcct ccgacaccgg aagcgaccaa
     7681 tacgtatgaa ccggctttcg aaattatgcc cccttcgtat atctctccat ttatgacaag
     7741 ttcggtcgta ttcgcaaaaa tgcggattcc ttccgaagtt tccatgccgt catatacatt
     7801 ctccaaacga atcgcaatcg aaaaaagata atttttttca tatccgcctt ctccgacgat
     7861 tgtcagttcg tggattccgg caatggaaac agttgatccg gacaaaaaag tggtatcatc
     7921 caaataaagg tcccccttag caaatattgt cacttcgtcc gtatataatt tgccgttttc
     7981 tattccctca acaagcgggt cgacgacgat attgaattct ttgattccga tatcgtcttc
     8041 gataattata tcgtaattac ccggagtgct tatatcgatc gattctacat acgtcccgtt
     8101 cacttgagct gttccctgat attgataatt gaaagtattc tcatacgttc cggctacgtc
     8161 tttttcataa caaagcgaaa gataactgtt gataatgtcg actttttcat tggcccactt
     8221 taccagcaac aagaaattcc tgttgccgta acatacggga atatcccgat aatccaaacg
     8281 gccctgaaca ttgccgttgg cgctgtaagt aacgatgctg ataccacaaa aaatgattcc
     8341 agtttcatcg tttaccatat atctttcata gacaacagcg atgttttttt ctagtacagc
     8401 cataaaaaca gcatgttcgc tcataccaga atcaaacatg acggtaatcg tttcttcttc
     8461 tgactttacc tgcaaatatc cgtccgattc aaccagaaac tctcccgtca cgtatccgta
     8521 tgaatataag attccctcat attcataatt gccaaggacc gtataagaat aaccgctcaa
     8581 atcttcttgt gccgtcatag gaagaaaaaa tgacgtcaag aacaacacaa atgttaaaag
     8641 ccgtctcgca agcataaaaa aacctcccgc gctttcatat tatgaattat acgcgcgaga
     8701 ggatttgttt ctttttctta cttcttgcaa agtgccaagg tatccctggc gatcatgact
     8761 tcttcgttcg tcggcaccac gtagactttt attttggaat ccggtgttga tatcagtttt
     8821 tcgacaccgt ggcattcatt caattccgga tcaattttaa cacccaaaac tttcaaacgc
     8881 ttgaccacat catgtctcat ggtcggcgca ttttctccga ttccggctgt gaagacgatg
     8941 gcgtcggcgc cttccagata aacgtaataa cttgctatgt aatctgctat gcgtttggct
     9001 tgaatatcga tcgccaactg agcacgggcg ttgccttttt ccgccgcatc gacgatatcg
     9061 cgagaatctg aagacactcc ggatacgcca agatatcccg attgcttgtt gagcatgttg
     9121 gtgatttcct tggcggattt gtttttttgg cccatcatgt actcaactac ggccgggtcg
     9181 atatttcccg tcctcgtacc catcggtatg ccttcgagcg gagtgagtcc cattgatgta
     9241 tccacgcatt tcccgtcttt aacagcgcaa agcgacgcgc cgttgccgat atgggcgatt
     9301 atcaatttag tgttttctgg tttctttccc aaaatttctg cggttctttg ggtcacgtag
     9361 cggtatgagg ttccgtgaaa accgtattta cgtacatgat accgctcgta ccattcgtaa
     9421 ggaacggcat acatgaatga ttcttccggc attgtctgat gaaaagccgt atcgaatacg
     9481 ccgacatttt taacatgagg caatatctcc ataaaagctc tgataccggt aagattcgga
     9541 accagatgca aaggcccgag atcgataatg ctttcgatga ttccgatttc cttttcggtg
     9601 atgatgcagg attcggtgag atagcttccc ccatgcagaa ttcggtgccc gactccttcg
     9661 atttcatcga acgatttgat gatcttcaga tctatcaatt tttccagcaa caaccgaacg
     9721 gcaacgctgt ggttgggaat atctttcttt tcctcgatcc tttcaccttt gtacttgatg
     9781 acgaataccg gatccttcat gccgattctt tccacgattc cgctagtgat cagagtttcc
     9841 tccggcattt ccaggagttg gaatttcagg gatgagcttc cggcgtttac tgccatgatt
     9901 tttgccattt tgtcatttat cctttctgtc tatataacga tcaaaccaat cgttgatatg
     9961 cgcaagtgtt tttttgaatt catcttgatc ggcgaacgaa gggatttccg caatcagaaa
    10021 attctcgttt ttctttttgg cgtccgtctt ttttctgagg attaagatgc tttttcccaa
    10081 ttttttaaag agatcgtcag gtaattttat caatccaata gccagatatt tcgccaatat
    10141 ggaatcacga tagtctctgg tgtcggtaat gtcgaaaaaa tcattcggta tcaccgaaac
    10201 gacataaccg tcattgatca gattatcata gtgatgttcc aaaaccttat agggaaaata
    10261 atcatgttcg gattcatccg aaaaaggaaa atcagtgacg atgatatcgg ctttgacacc
    10321 ctctaaagat aaagtatccg caagaattat gtttgctttc tcgccaagca tgttcatcag
    10381 cgccatagcc agattcccgc attccttgtc tatatcgcat ccgatagcgg tagttttgcg
    10441 gtcaagattg ttcacgactg aagctaggag gtttcccgtt ccgaccaaag gatcgaagat
    10501 tgtcggttct tccggaacta ccgctagtct ttcaaccaaa tacgatacca acacgccaat
    10561 ggtatccgga gtgatgaacg aattggatct gccttcttgt ttcaaccttt tcaatacggc
    10621 cagttggaaa gctttgcgaa tttcttcttt tcctagtttc agttccttga ctgattcgat
    10681 tatatttctt atttccttgt cttcttgatc agcagcaaaa atgacgttgc aagccgtcgc
    10741 aacgccttcg atgccgttca tttgcctttt agcggaataa agcgaagcaa tttcgtcaat
    10801 tgcgtagaat aatttctcga tgttgttatg atcaagcata gcctcacctt ttgttaattt
    10861 tatcataggt tagccgattt ctcaatacaa atccggtctc cagtttctat tgtgaggacc
    10921 ttgcttgcaa aagtgtcctc atgttggtaa aattaacgat ggtgaaacca ttatgagaaa
    10981 acttgcaatc aagctgatca ggctttatca aaaggcatcg gtaggaaaaa gacctacatg
    11041 ccgatatcgt ccgacatgtt ctagttatgc cattcaatgc taccaaaact ataatgtttt
    11101 ttccgcaacc attctaacaa tatggcgtat tttacgatgc aatcctctct ctcagggagg
    11161 ctacgatccg attccaaaag tgaaaaaagc tgaacgtgaa cgtataaaag gctgatgcgc
    11221 caaagtgttt gccggccttt tcaatactgg aaaaggtaaa aacaaaaatc cataaagaaa
    11281 gtcataccgg aattgaaatc cggtatgact gtttttatat tgtgtttctt tttgattgtt
    11341 tgttaattgt tatcgctatt cattttcttt ttgaaagcga caatggcaat tgcgatcatc
    11401 gcgacggcat atccgatgac ccaccacaaa tctggcatta tttcactata attaccggaa
    11461 agcgctgctc ttccagcatc tgccgcatga acaaaaggca atgcctcagc cacgattttg
    11521 aatcctttcc cgaccagatt gatatcgaac cacgttccgg aaagccaacc ggtgagattg
    11581 gtcaacaacg ctccgcatat tccgccaacc tgcttatcgg taaaaacgct gccgcaaaac
    11641 agaccgatgg cgataaaaaa caaaattgcc ggaacggata caaaaatgca taacagtata
    11701 ttccaggtcg ccccaaggcc aagcaccatg gcggccaaaa aacatatacc gatttggaca
    11761 atcgccatcg gaatcaaagg cagggtgtat ccggcgatga aatcgcctga tgtcatcgga
    11821 gaagaaaaca ttctcatcat gaaggaactg cttctgtctt tggcaatcag cacgctggaa
    11881 aaaagcgaaa tgaaggaaag cccgaagatc gcgatccccg gagtcatttg gtccagttcg
    11941 aataattcaa ccggaacatt tgcctgaatc gccgtcagaa gcataatcaa aaccagcgga
    12001 aagccaagcc cgaatgccag attgagagga tccctgagta tttccattgt gtttcttttc
    12061 gcatacgccc atgctttcat ttgccatcgc cttcttcccc aacaatagca atgaatgcgt
    12121 cctcgatatt tgccgcattg gttttttcct taagttcgac cggggttccg atcgctttca
    12181 cggatccttt ggccagtaca caaatccggt cggacaacgc ttccgcttct tccatgtagt
    12241 gagtagtcaa aatgatggtt atctttcctt taagggattt gattattttc cacagttccc
    12301 ttcttgccaa aacatcgagt cccaaagtcg gctcatcaag aaacaggact tccggctcgg
    12361 tgatcaaagc catggcgatc cccagtctcc tttgccatcc cccagaaagt gttttggctc
    12421 ttttcttggc gacttcttcc attccatact tctgaatggc attgtcggcc attcttttcg
    12481 ccgcaatcgg atcataaccg tatacgccgg caatcagaca gagattctca tagacgttta
    12541 gattggccgc aactgccgtc tcctgcgggg aaatgctgat tttttctttg actttgatcg
    12601 gtgaagaaat cacgctgtca ccatggacca gggcttctcc cgacgacggt cttgtcaagc
    12661 aggacagcat tttgattgtc gttgtctttc cggcgccgtt cactcccaaa atagaaaata
    12721 attcgccttt ttcgactttt aagtttaatt tgtcgacagc caatactttt ttatactttt
    12781 ttgtcaggtc gaaagtttcg atggcataca tcttatttat catccttacc gttcaggctt
    12841 ttgattcccc tggcaaaaga catggcatct tcttcgctga catcaggata tatttcctga
    12901 ctttttccgg caaaaatggc atcggctatc ttattgaaaa cattcattct ctcgatagcg
    12961 atcccttttt ccttgtccgc caaaatgaca agcgagtctc ccggtttgat tccgaacatg
    13021 tccctgattt ctttcggtat caccacttgt ccctttggac ctacccttac ggctccgata
    13081 cacttttccg acattttctc tttttccaat atggcacctc cgacacggta taacttgtat
    13141 aattaattat accaccgtga attgcaaggt caataaaaac aataaaaaaa atcgatcttt
    13201 ttcagatcgg ttttctttac actgatatca atatcgcatt ggtgcgggtg acaggacttg
    13261 aacctgcacg tctgggacac cagatcctaa gtctggcgcg tctgccaatt ccgccacacc
    13321 cgcaaaagat ggtgacccgc aggcgattcg aacgcctgac cctttgatta aaagtcaaat
    13381 gctctaccga ctgagctagc gggtcattaa aatggctggg ctaggtggat ttgaaccacc
    13441 gttctcggag tcaaagtccg atgccttaac cccttggcta tagcccaacc tagcgaaaaa
    13501 tggggcgatt gatgggactc gaacccacga atgccggagc cacaatccgg agcgttaacc
    13561 agcttcgcca caaccgccat cgtctttttc gcacctttta gtttactaaa taggcctata
    13621 aaagtcaagc aatatttcca agtaacatca gcggcaacga taaaaaaaga cgcccggtaa
    13681 atggcgcctc aaactcatgg tcattcagca gctacggcaa gcccgaatcc ttggtctccc
    13741 aagtgcgcga agaagaccgg agaaacgtag tccgtcatgt gaattccggc agccggatat
    13801 ttgccggaaa tatacaatct gattttctcc gcccattccg gcctgttcaa atgaatgata
    13861 tcaaaataca cttttttgaa tttctggcaa tattcatcgg ctttattcat gaagtactcg
    13921 aaacatgcca aattggttct ttcttttttt gtaagttgca atttcccttc gaccatttcg
    13981 acaatcggtt tgatcctgag tacggttcca agaagcgcct gaatccggtt aagtcttccc
    14041 ccgcgaaaaa gattcatcag gtttccgagg gtaaacatca cggctccgtt tttgaatatg
    14101 gtataatacc gtttttgcac ttcttcgaac gaagcgcctt ccgccaccat ttccgcaagc
    14161 aacgccactc cgttggcaac accgaaagac gccaactgcg gggcgtgaat actgatgtcc
    14221 atttcaaaat cgatcatggt tttcgctatc atggccgatt gatatgttcc gctcagtttg
    14281 tcggaaatgg tcactaccaa aacattcgtg tacccttcgt tatggaattc ctcatatttt
    14341 tccaagaatt ctcctggcgc gggctgactt gtcgttatat gcttggcttt atgaagttgc
    14401 tcgatcattt cctcggttgt aatatcttct tctttgcgag atgttccgtc taatgtaacg
    14461 taaagcgaca ctatcttgat gaacggatac tctttcaatt ccgctcttgt caggctggtg
    14521 ctgtcaatca aaatacctat tttttccatt ttctcacctt gctattttgc atatgaaatc
    14581 acatagtatg acatcacata cataatactg tatcaaaaac ccatgattta atcaagaaaa
    14641 acatacaaac atcaaaaaaa gaagcggccg agccgcttcc ttgattaatt gtttttcttg
    14701 tcgtgttctt caattatctt gccgatcaga tttcctggta cttcctcgta acgaacgaat
    14761 tctctggtga aagttcccga tccctgtgtc atggctttaa gatcaatcgt atatttggta
    14821 atttccgcct gaggggcttc agccacgatc ttctggtatc cggcttcagc tggttccatg
    14881 cccattacac gaccacgacg tttgtttaca tcacctaaaa catctcccat gtattcatcc
    14941 ttgacggtga cgacaatctt gtcgatcggt tcgagaatag tcggctttgc ttgtttgcaa
    15001 gcttccttga aggcaatgga tgcggccatt ttgaatgcca gttcattgga gtcaaccgga
    15061 tgatatttac catcggtcag aatgcctttg actccgatca ccgggaatcc tgccagggtt
    15121 ccgtgttcca aagtttcctg caatcccttg tcgacggccg ggaaatagtt tcttggtacc
    15181 gatccgccga atacttcttc cgtaaattca tagtctttgt cgggattcgg ctcgaaacgc
    15241 atgacgacga cgccgtaata accggaacca ccggattgtt tgatatatct gccttccgca
    15301 gttgcggtgg ctttgatcgt ttcacggtag acgatttgtt gctcttccgt atcaatgtcg
    15361 actttgaaca tatttttcat cttatcggtg atatagccaa gatggattat tccctgtccg
    15421 ccaatcagca actgactggt ttctttgttg cggcggatct cgaaagtgtt gtcttcaagt
    15481 tgcagtttct gcaacgcatt cgacaatttg tcttcatcgg ctttgttctt cggatggatg
    15541 gcgatataca tcgtcggggt gggggtatat gttttcggat aaacgatcgg ggatttctta
    15601 tcggacaacg tcgaatccgt cacgactccg tcggtcttgg caattccgca aatgtcgcct
    15661 gcatgaacca cttccaccgg aatctgggtc ttcccgcaaa ggaagaacag attaccgatc
    15721 tttctggttt cctgaacact cgacacatat acttcctgat ccttgtgaac tgttccagaa
    15781 cgtacttgga tgatatttat cgctcccatg aatggatcga ttgttgtctt gaaaacataa
    15841 gcggagaacg gctcggtatc gcttactttg cgactgatga catcatcgct gtcaggagcg
    15901 cttcccttga acgactcggc ttcttccatg gatggaaaat actcatgaat cataccgagc
    15961 agggaaaccg atccgatgtc cttcgtggcg gaaccgacga ttaccggtac aacctcgcct
    16021 gaaagaacgg tcgtatgtaa accttttttg acttcttcca atgtgagctc ttcgccgccg
    16081 aaatatttct ccaacagttc ttcgttggat ccggcaactg tttcgacaag ttcggtgcga
    16141 tatccctgga ccttatccat cttgtccggg tagatttcgc cgtcgacaac agattttccg
    16201 tcgtaaagtc tggcttttaa atcgatgatg tcggcaaagc cgctgaattc atcgcttttt
    16261 ccgatcggca cgcaaaacgg cattgcctgt tttccgagtt tttctttgac ggcagtcatt
    16321 actgaatcga atttgacgtt ttccttgtcc atcttattga caaaaatgat agctggaaga
    16381 ttgcgctttt tgatttccag ccacattttt tcagctccga tttccacgcc tttggtggca
    16441 tccagaacca ggatggcacc ggaagcggcg ttgaaagcat aggaaacgtc gccaatcatt
    16501 tcatcggcgc ctggggcgtc gagaacattt attttgatgt catcgtattc aacagggaca
    16561 acactggtag ataaagagtt ttgttttgcc tgttcttccg gagagtaatc ggaaatggtg
    16621 gtcttcttct cgacctctcc ttttttttct atccgtccgg tcacataggc cagagactcg
    16681 acaagggacg tcttgccgct tccaaggtgg cctaaaagaa tgacgctctt caaggatttt
    16741 gctgaatatt ctttcattta tgttgatctc ctctcgttca actctgtttt cagtctattt
    16801 tattatatca tatctaacgg aaaaataaag atgataatat cagcgctttc atggaaaagc
    16861 cgcccttggg cggcaaggcg tttcgacgca atatccatat aggttttttg gtttatcaca
    16921 tagcatatta aagcgatcgg tatgataatt atatcataac aggcgttatt ttcccattaa
    16981 tcatttttcc ccggattttc ttgctttgcg atcatggtgt atttcctgac aaaatcggtt
    17041 ttggcttcgg tatattcatt gcgattgaat tcatattgct tccataattt cagtttaagt
    17101 tcctcatatt ccttggcgat atccggatgg cttgtcaggt aatttctaaa atatatttcg
    17161 tcgttatcgc cctgataact gacatggata tggaaaaccc tgtccgcaaa tccctgctcg
    17221 gtatatccct tgttaagaga taatctattg tcttcatcgg acatgaatcg ataaccattt
    17281 tttacaagca aatccttaat ttgagacatt ggtttgccaa gccggatttc caccagaata
    17341 tcgacaatgg gtttagcgtg aattccgtcg atcgatgtgc tgccgatatg actgatcctg
    17401 acgacagcgt ctcttggcaa aatgttttcc aagatagctt tttcttcaat aaaccatgat
    17461 ttccaatatg ttttatgact ttctaagaaa atcgggaaaa cacaccacag ttcctttttg
    17521 gtcatttcgg ataactttct tcccgtcttg ccgccagagt tggaatacag ccaatacatt
    17581 tgatcgattt tacccttata ttcgaactct tctccaagaa ctccgccgtt tttcattata
    17641 cttttggccg aagcaatatt atccttttca catatcatca aaactctgtc gatgccacgt
    17701 tgtttgcata tatctagagc cagaccgatc atttctgtcc cgtatccttt tcctctttcg
    17761 cttggtctga taccgtcgcc aatatgactc ccgccgttcg caagcattgt ttcttcatcg
    17821 ggataaagcc gtatattgac ggctccaacc atgagatttc tttccaaatc aagacaaaag
    17881 taagtcagag ctggcacttt cccaagaggc ggttcgctct cttcttttct gaatccttca
    17941 agataaccat taaaatcgcg ataatcgtcc ctcgctatgc tccacggagt aatggacttt
    18001 tcttttgcgg tccattcatc catcatttcg acaatccgct tttcatatac tcgacttggt
    18061 ttgacaagtt tcaacatcca caatcaccgt ccggtttttg tttgttccct tccgattata
    18121 gcatacgtgt tattgccatc aaagacatat taaaaaaacc aggcaaatgc gtggcttgac
    18181 tatctgtttg gcaggggtga caggattcga acccgtacta gcggttttgg agaccgacgt
    18241 gctaccattg acaccacacc cctaatggtc tagttaatat tatctttcaa tcttggtttt
    18301 tagtcaagac ttttttccgt agtcaaaacc aggtaaacat ttaccggttt ttctcgttct
    18361 tgatcatttt cagacgagaa aattcctctc gttctttttc ttccagcgaa tcggcaatga
    18421 atttgatatt ctcctgcaat tccggaataa cgatgttttt tagagcgttc gcccgtttct
    18481 gtgcttttct gatcgcaaaa gcaagccggt aggcgctgtt gtcgatctct gccagtttga
    18541 ttaccaaatc tcggactttt ataaaattct gatatgcata atcgaatttg gtattggtgc
    18601 tggcgatgcc gtaattcagt cttaccggtt tcttgtcata cgaaagactg ggaatatcca
    18661 ctcccatgac acttctgtat gttatggata gcccgtcgtc gatcggcact gatttggcga
    18721 gatcgctgac tattcctaac gtaatattgg cttcctgcaa agcaatatat gctgtttgga
    18781 aaatatccgt gatttcggtg cgcatctgtc tgacgctttc tatcagttgc atcatttccc
    18841 tgattaggat gtttcgcttg cgatcgatca gatcatatcc tagtttagcg agctgatctg
    18901 tttttttgaa cgccagcaga ttccctttgg tgggaaaaac ctggtttttt gccatggtta
    18961 tgattcactt ccccggtcga gttccctgat aatcgattta tccctgattt taaattcttt
    19021 ttttgccgcc gccggattgt agtatttgtc aaggtactcg tcttcggtac ggtcgagttc
    19081 ctcttttggt aaagtggaaa gaagtgccca tccgaggtca agggtttctt cgatcgaacg
    19141 gttctcgtcg tgtctttggg tcaaaaagcg actctcgaac agtctgccga actcgatata
    19201 cattttatcg gtttcgttca gttcatcctc gccgatgacg gctgatagat ttctggcatc
    19261 ctgaactctg gcatatgacg cgaacaattg gttcatcagg gtctgatgat cttcccttgt
    19321 gtaaccggcg ccgattccgt ctttcattaa cctggacaga gacggcaaaa cgccaatcgg
    19381 gggatagatg ccaagatgat tcatttcccg atcgagaacg atctgtcctt cggtgatata
    19441 tcctgttaag tcgggaatcg gatgagtaat gtcgtcgtta ggcatcgtca gaatcggaat
    19501 ctgggtgact gatcctttgg catctttgat gataccggcc cgttcataga tggaagcaag
    19561 gtcggaatac aaataacctg gataaccttt tctgccgggt atttcttcct tgctgctgga
    19621 aaattcccga agggcttcgc aataagacgt gatatccgtg gagatgacca aaacatgcat
    19681 gtcatgttta aaagctaaat attcagctgc ggtcaaagcg caacgcggag tcagaagtct
    19741 ctcgatgatc ggatcgttag caagattcaa aaacatcaca accctgtcaa agacaccagc
    19801 ttcttcaaat gaacgccgaa aataatctgc cacgtcgttt ttgacgccaa gagcggcgaa
    19861 cacgatacag aagttctttc cttcgccttc ggcgatctgc gcctgtttga ctatttgaac
    19921 agctagttcg tcatgtggca ggccggaacc ggagaatatc ggcagtttct gtcccctgat
    19981 aagcgttgtc aaagcatcaa tggaactgat acctgtatga atatagttac gcgggtaaac
    20041 acgggaaacc ggatttaaag gcaagccgtt gatatcgcgg ttttcatccg cgaaaatcgg
    20101 acctagtccg tccatcggac ggccggaacc attgaaaacg cgtccaagta ttcctttgga
    20161 aagggcaatt tccatcggat gtccgagaaa tttggtcctg gtgttttcaa gcgaaagccc
    20221 gaaagtgccc tcgaaaacct ggatagccac tttttttccg gaaatcaaaa cgactctgcc
    20281 ataacggatt tctccgcttg tgagcctaat ttccaccatc tcttcgtagc cgatgttttc
    20341 aactccgtcc atgaagatga gcggcccgtt gatttcatcc aagcctatat attgaaggtt
    20401 catgataatt cctcctattt gacgctttta acgacatcgt caatcatggg aaaataatcg
    20461 tcgaacattt ccaatttgtc attcggaaca tcgtatttca tcttgacgat cttttcgaaa
    20521 atcttcgtct tctgaatcaa actgaacgct ttgccttcct tgaggatctc tacacacttc
    20581 tgatacaaat acaaaatgac atccatcatc cggagttgct tgggaagcgg aacaaaaacg
    20641 tcgtccttgt ggaaggcatt ctgttgcagg tatccgaccc tgatcactcg gctaacttcc
    20701 aaaatcagcc tttggtcgtc cggcaacaca tcgcttccga tcaatttgac gatttccatc
    20761 aatttatttt cttctgtcaa caatttcata atttttccgc gattgtcaat gaaacgcggt
    20821 ccgacgttct tgttatacca tttggcgaga tcaggtacgt attcggaata actgatattc
    20881 cagttgattg cttcataatg tctggcatag gcaagttgtt tgtcaagcgc ccaaaaacaa
    20941 cggacaaacc ttttcgtatt ttgagtcacc ggctcggaaa agtcggctcc ttggggagaa
    21001 acggccccga tgatcgatac cgatcccaag gttccattga tgttggacat gtatccggcc
    21061 cgttcataga actgggatat ccggcttgga agatatgacg gaaatccttc ttccgctggc
    21121 atttcttcta gacgcccgct gatttcccgc aaggcctccg cccatctgga agtcgagtcg
    21181 gccatgacag ccacattata tcccatgtcg cgataatatt ccgcaaaggt aagcccggta
    21241 taaatgctag cttcgcgtgc cgcaaccggc atgttcgaag tgttggcaat gaatacggtc
    21301 cgatttgtaa ggggtttgcc gcttctcgga tctaacaatt cgccgaattc ttccaaaacc
    21361 tgagtcatct cgttgccacg ttcgccgcat ccgatataaa caatgatgtc ggcatcgcac
    21421 catttggcaa gttgatgctg catcatggtt ttaccggttc cgaaccctcc cggtacggcg
    21481 gcagtgccgc ccttggcgat cggaaacagg gtgtcaatga ctctttgccc ggtgataagc
    21541 ggagtcgtta tctgcaatct ttccctaaca gggcgaggtg tcttgatcgg ccagcgctgg
    21601 acaagggata gttcgacggt tgacgagtct tccgtcttga tttttacgat agtgtcgttg
    21661 actttatatt ttccattccg cttgacttcc acaacggttc cgccgattcc tttcggaacc
    21721 atcagtttat gtaaaacaag ttcggtttcc gaaaccgtag catatatatc atcgaatgcc
    21781 aaaacatcgc cttttttaca aagcagtttg acttcccatt ccttttcttc atccagtgaa
    21841 ctcatttgaa atccggaagc gataaaagct ccgtattttt ccgatattgt ctttaacggc
    21901 cttccaatac cgtcgaatat attttgcatt attcccggtc ctagggtgac cgaaacggga
    21961 cttcctgtac caatgaccgg ttcgcccacc cgaagggaag tcgtcgattc ataaacctgg
    22021 atagtcgtcg cgactgaagt tatcttgata acctcgccaa gtagtttttt attgccgaca
    22081 aagaccattt ccatcatgga aaaatccgtg gtatccctta cggtaaccac cggtccgttg
    22141 atggagaata tcttgttcat acgatcacat ccttaccgct tgtgtcaaac ttcattcgaa
    22201 aagcaggctt gcccgtgaat aaaaccattc tttccggctt tccaaagccg aatcaattgt
    22261 ttcgtcgatt tgaagcgcat gtttctcgga caaagcgaca aatccgccga ttttgattgc
    22321 tggatcctcg gatacggtat tttgttcctt gcaggtatcc ttgattattc cagccagatt
    22381 ttcatcacct ttacgaatcc ggaaatcaat cttccctccg gataacttgg tataaattcc
    22441 agcaagattt gatttcatca ttttagtata ttccgggcca ttcgcaaaat cctgcaattt
    22501 tgtcttggct accgcaaaaa tctgatccat gtattcctga cgcttttgca tcagtttgtg
    22561 cgaatagtcg taggaaatac gattgagttc ctgactatga tcggtttcca tatctctgat
    22621 tttagccgca agttcggttt gcgaagattc cgaaagatca gatttgatct tttctagcgc
    22681 cttttttttc aaatcgtcga tttccgcttt gagtgccttc atctgtttcc cggcgttcat
    22741 tataatttcg tcttcgaaat atttataaag ctggtcgttg gtataataat ccattggttt
    22801 tcacctcata tcttgatgcc gattgcttca ctgacgtatt tgtcaatcgt ttctccgata
    22861 ttcgtaccgc catggcgatc cggtatctcg acaatcaaag gaatatctct cgtcagtttt
    22921 agttctgata taacttcagg acaaagttcc accaatttgg tggtaatcaa aactatgcag
    22981 ttttccttgt cctgcatggc cttttcaagt tccaccagga cttcctggcg caggtggaca
    23041 acgacgcctt caataccgac aagccgcatg ccgacaaggg tgtcgacgtt gtcgctgatg
    23101 acaaaaaatt tcatgttgtc cttgtaactt tcttaaagct tgttgatgat caaaatcgag
    23161 atcaacaaac catagatggc gattccttca cccaaagcca caaagatcat cgacttaccg
    23221 aatgtctttt cattttccga taaagctccg atggcggccg gtgtaccggc tgcgacggcg
    23281 attccggctc cgagcgatga caacccggcg gccagagctg cactcagaaa tcctagtccc
    23341 tgggcgatgg aaccgtcaaa tgccgtcgtt tcgccggcag ctgcgaccca aacgcctttt
    23401 atttcaaaaa ccgcggtaag aatcaataaa ccgaaaaatc cgaaaacctg tcccaaaacc
    23461 cgacgtttgg cggaaatgaa agacactttt ccacgaaaca cattgacaag gggcaaggaa
    23521 atcaaaatca gcaaagcgag cggcaacaac cattcaaaac cattcaacat aaaggtacct
    23581 cctaatatat ctcaatttta ttttttctcc atttattcga atatcgtaaa ggcgacgccg
    23641 tcgccttcat aataacggga aaacatttca tagtattcca gacgcaaaac ctgaatcccg
    23701 acaaccagcc cttccaaagc catcacaatc atgttcccga acaggaacac gagcgcttct
    23761 ccgacaaagt taccgacaga tgacatcaag gtatatacca ccaacatcat tccggcgtgc
    23821 gacaatacga atccgcccac acgcaggaaa gacaccgtgt tggtcagata tgacaaagcg
    23881 atctcgtaaa gatcgaagaa cgtatcggcg ataaacccgc ctattctgcc ggtgaaaaat
    23941 ttctgaccgg caaattttct gacgaatggc tgtttcaata tgatcattaa aaccggtatg
    24001 ccgataaaca ataacaaggc ccacagcgta aagaaactca ctccgtaaaa aaacttcgcc
    24061 agtaggctga ccgccacgta agaataaagg atcaaaccgg caataccgtt gtgggaaaag
    24121 atcatttccg catattcttt cttcttgcgt ctggtcatga tattgatcag gattgatgtg
    24181 atgatcaatg cggccccgat gataaccgcc agagtcaaca gggtggaagt gaaatcatta
    24241 ctcaaaacat caactggctt gttttgcatg ccaaacagtt tgtagagagg caaaagcaaa
    24301 gtctcgtttc cgaaaacaga gccgtagatg aatccgaaga aagtcgaaaa catgccgata
    24361 cggatggcta tttgtcccaa actcgatttt ttcttcctgg ccagcaatat ccccagaaga
    24421 accaacacaa gtccttggcc gacatcaccg aacatgattc caaaaagaag agaatacgtc
    24481 accgcaaaaa atggcgtcgg atcaatatcc gcatacgatg gcgttccata catattgaca
    24541 aaagtcgaaa acggtctggt aaaccaattg tttttgagct ttgtaggtgg caccagccgt
    24601 ttgtcggccc cggccggacg cactgtcact tctacatcct ttatgtttgc gaatctttct
    24661 tttatccgtt caacgtctgt cgcttcggca aatcccgtta ttgagaattt ttctcccaaa
    24721 ttaacgacat acttctttgc ttcgttaatt ttagctaaga acagcaattc gtttttaatg
    24781 gcaacaagtt catgttttga ttcctgcaag aaacttgtta tttctgtttg caattcagaa
    24841 gattccttgc agaaattatt catttccctt gtcaattgag tctcggcgct ttccggcgtg
    24901 ccgtggacaa attccgggat atggatgcgt tcgaagaaca aagacgagaa gatattgtca
    24961 acttctcttt cgtattccgg ggatgtgaaa tacatacacc aactgtattc cttgtcggtt
    25021 ccgaaagaat tgaaaataaa gggccgggat cgataatagt ccaatttttc aatgctgctt
    25081 ttcggtaggc gtcccaccct tgcaaaaata tagcggcagg aaaacagatc atccaaagaa
    25141 atatccatgc tttcgatgtt tctgacctga accaaggcat tctcgtattt ttccgtttgt
    25201 tcctgaagtt cttttttctt ttcgatgagt tctttcagtc tcttgtgcgt cgtagaaatg
    25261 taattcttca tcttatcggg agaatactcg gttatttccg tcccgtctgc cgaaatctca
    25321 atatcaaatt ctttttcgat ttccgacaac tcttccaata tcgtttctgt cggattctct
    25381 tcggaaatgg ttctgagccc gtgtactctt tcgacaaatt tagaagatag aaccggactg
    25441 aagcaatgca aagaaacgaa tttcgccaag actggttcga gattcgaaag attcgaagtg
    25501 atgttgatca atttgagttt tgcgattgcc atctgaatga agatcctttc aaaacacgtt
    25561 ggtccgtaat atcagatcta tgactaaatc aataaaaatt ctcttttttc agtatcttcg
    25621 gtgggatcaa cctggcatcg gcatcggccg gcatgatttc cagtcccagt ttgggatttg
    25681 acttgatcat ttctgtgatc cgatgaacat ccttggaacg aacaaatccc gaaatcgata
    25741 tttttgaccc aagcacgaca acatacttct ttgccgcatt tacccggttc aaaaactgca
    25801 actccccttt gacttcagat aactcttctg atgcggtcgc taaagcattt gtgatttttg
    25861 tttcgatttc ttccagttct tttgcgattt ttcctttttt gtccgctaaa gtcattgccg
    25921 cctgtttcgg cgttccgtgg acaaaatcgg gaatgtagaa tcgttcgaag aacagggatg
    25981 agaatatgtt gtcaacttct cgctcgtatt cctttgatgt gaaatacata caccagctat
    26041 agtttttgtc ttcggcaaac gatttgaata taaagggttt gtcctgataa aactccagtt
    26101 tctctacact gtcgtttggc aactttccaa atctggcata cacataatcg catgaaaaaa
    26161 tgtcttctaa cggggtattt agacccgcca gggtctttac ttgtttgtag gcttcctcgt
    26221 atttgtccaa ctctttcttc ttttcgtttc tcttgttaac caaatcggat aactcggttt
    26281 ttattttatc aatggctttt tttgctttga taattcccat tgttgatgtt tttactatct
    26341 ttggttcggc aagaaaagaa aactttttct cgatttccgc gatttctccc agataagccg
    26401 gacacggatt ttcatcggat aaagccgaca gtccgtggac cacatcgaca aatttgatcg
    26461 catcgaccgg agcaaaacaa tgaatgttcg caaaagacgc caaagcggaa tcagcattgg
    26521 ttttgtttgt ggatatgact gccaatctga tttttgacaa tgccatcttt tctgcttctc
    26581 ctttttaaat caataaatca gcattttcct gatttcgctt tctggaaggc cataacggat
    26641 accttcgatg atatggaaca aattctgcct ttcgatttct tccatgatca ggaatgcgga
    26701 aaaaacaatg ggggccgaat ttgaataata tagatctttt tttgccagat gatatctgat
    26761 tttatcgcca aaatattcga cataaacaaa atcgttatcg tcagcatagc gggcatactc
    26821 cgaatctctt agaaaggaca atatcccatc ggcgtcggta ttttcgataa tttccctagc
    26881 tctttttttg cttgttctga tatgtttgaa atccatcaaa ttctcgattt catcgggact
    26941 ggcatcgtaa aacttcttca gacgatatgc caaaatgata ttggcaagct cgattctcgt
    27001 catgaacata atttccaatt cttttctcac gctgccggaa taattctcgg cgatacgttc
    27061 aaaaacgcag tcgtaatagt gggaattgag tttttgctcg atggccgagt atttgatgag
    27121 attgcgatcc gtttcaaaat atggttccaa gatcgcttga tatttggtgc ccttgacagc
    27181 ttctagcagc atgccgtaat cccttacccg ggaaaaagcc gtgatgtcaa aactcgcatg
    27241 tctgatgaaa tatgtcggaa attcggcgat ggcgatatcg atgttttctg atatgagcat
    27301 tcttatcgtg gccagaataa gatctatttc tctatggatg atattgagat gataatacga
    27361 tttatctttc aattccacga atttggctac tttcaacgta tggagaaaat tgttcttctt
    27421 aatcaactct tccaattgtc cgcggtgaat cgatgattcc tggatgtcgg ccagggtttc
    27481 ctgataatct ggatgatttt tcagatatgc ggctatttcg ccaaccgatt ttttcttact
    27541 gagttcttca aaatcctccg ctttcaaaag cgtgccataa atggctttgg ttttagcgac
    27601 catggcgttt gcggcaaaag accccatcgg tttcacttcc ttcggctaat ttattcttcc
    27661 ttcagacaag cattataaac ctgatcggtc cattcctgaa aatgcgcctt gaacgcgcct
    27721 tccattttgg caatcgattc ctgataagaa atttcaaatt cacgtttcct cttatccatt
    27781 tctttatcca atttatcttt tttgtcagta agcgcctttt cggtttggcc cttgtattcc
    27841 gcttccattt tggttttttc atcacggata tacgcgtcaa ttttatcttt ttcttttcgc
    27901 aaggcatcta ctttcaagcg atattctttg tccagttcga caatgtcctt gatcagtttc
    27961 tccatatcgg acgcctccta aagtatgtat gatatgacat gctatatatt atagcaccaa
    28021 aataaacgat attcaaaacc ttgattcatc tctttttggc cagtaaaaaa aataacagcc
    28081 cttgcgggca tgttattctt cactgatcaa ttccaccaag tggtatcctt cttcttccaa
    28141 ggtggctgcg atgacttttt tggaaacata tcctttaaaa gtcgcaactc cgtcctcggc
    28201 tgatacactt acatctgtca ctccgtatat tccttccagg atatgctgta catccctggc
    28261 gcatccattg caacaaatac cttcgattat cgcctttttc atatctgctt cacttctttc
    28321 ttatgtcgaa ttgttaacta cctatcaaaa tcaaaccatt tcgttttcgt ccggttcata
    28381 gaccgttaac ttgtttttgc ctgtgttttt cgatctataa agcgccttat cggccaagaa
    28441 aatcagatca taaatatcta tcggtttcga accggaatgg ttatataatc ccaaactgac
    28501 tttcaaatcg atctttttgc cttcgaaact gtattccttt ttcatcgctg cgatgcgaat
    28561 cccctgggcc atagcgattg catcttctct ggaaatgttt ttgagaagaa cgacaaattc
    28621 ttcgccgccg taacgcgcaa acagactatc gtcgggaagc aactcgaaga ttgtttcggc
    28681 aaatgtcttc aacacataat ccccggccat atggctataa gtgtcattga ttgatttaaa
    28741 atcgtcaata tccatataga tgatggagaa ttcagcgtgc cgtttgttca tgcctgccag
    28801 atattccctg gctttttcat gaaatgcccg gcgattgaga attttagtca attcatcgtg
    28861 ggaagcctga taatcaagtt tttcgatctt atcgacaaag gtcgttgttt cgttgaactt
    28921 tagcatatat cccttcaaac tgcgactgat aggagatatt agagaaatgg tttccaatac
    28981 ataatgcttt attcctttgg tgaaactgaa cggttgcata tcgattatgg ccgccagcat
    29041 ctccctgtcg cttccgaaca tttgttccaa aggcatatgg aggtaaggcg gattctcttt
    29101 ttccataaat tcggtcgcgg ctttgttcca tgtgaccaat tccctttgtt cgttcaatat
    29161 catcaaaggt aaggtgttga tctcaaaagc gtgctggtaa gcctccggct gcaaatcgaa
    29221 tagttcatac cggaatatcg cataaatgat gatataactg ataaaaacaa gaatatagag
    29281 cgaccaatcg atcccggtcg tcaccttggt gaaatatgcc acgaacgatg aaccgagtcc
    29341 aatgatggaa cacatcgcca tgatcaggga ttgtttcttt ctggttccat gagtttgcat
    29401 gtagttgttt atatagatgt atgcgacata tgccgtcaag ctggcattat acgaaatatt
    29461 caaaaaatat aacggccctt tcgtaaggga aagaacggta acggtttcaa tgccaaagaa
    29521 agaaaattgg gttaattccg cttccgaata ataaagatcg aagccggtga aataagatat
    29581 ttgaaccgac aggaaagtaa taaaaggcac aattaacaaa ataatataat ggttaagttt
    29641 tgtgctgtac tgggcatcgc ggaatttttt tgcaacatag atccaaacca caacgataaa
    29701 cggtatggca aaatattgaa tatgattgaa tatcactttg atgactatgt cgttcgatgc
    29761 cagctccatg gcgtacccga aagcataaat gaattcgaga aaaaacagca cgctgacttt
    29821 ccagaaaccg tgagcggatt taagtttaac aagaatataa attccgatga aaagagaaat
    29881 caatgccatt gcaaatgtaa atcccactaa caacgcaatg taattcatcg gatcacctcc
    29941 ttaaaacttt tacactccca atttgtattt ttattatatc atttgtattt caaaaaatat
    30001 aataaatctt gaaaaagggg gacggatatt tattgcttgt cggtttcttc tggtatctta
    30061 ggcgaaaaag ccagaaaaaa cacaaaaccg aaagcaacgg ttgcggctac gatttggtat
    30121 ggcattgaat aaccgttggc atctgccagc aatccgaaac catagttgcc gatagcggtg
    30181 aaaatagaaa agaaaagcgt aattatcaat atggccgtcg taacattttc cactttgacg
    30241 atttttatga tgtaatgaat gtttgcgtaa attagaattc cccacgaagc tccccttaac
    30301 aaggtcaccg ggattattac cgaaagcggc aagtcgaacc cataaaaaat aaaacgggca
    30361 acaaagagaa aattggccag aaccagcaat ttcctttctc ccatcgattg tcctttcgtg
    30421 atcattatac gcaaagtgat cacctcgaca aaaacaaagg ccgcatacaa taatccatac
    30481 cattgggatt ccagtcccag atcagtaata taaacaccaa aaaacgaatc gccgattctc
    30541 acagctccga tggtgatagt gtaatatatc agatacttgt agaaattttt gtttttcaac
    30601 agtgacttga tatcgcgctt agtatcttcc gaaacttccg catccttttt aaatggtttc
    30661 aaccaaaata caatcagcga tgtcaaaatg aaaaacgttc ctgaaatgat aaacacgctt
    30721 tcgtaaccaa accaaagaat cgccgagccg gcaatagccg acgccacgac ataagcgatt
    30781 gaagcgtata ttctgatgga agaatactca attttctcgt tattgcagaa agtggcggtg
    30841 aacccgtcct ggatatttat gaagggagaa caaaagaacg cgatgatact gacaatcaga
    30901 gcatacattt caaatccgga aatctgggtc atggttatta tcattgcgcc ttcgataacc
    30961 gtcattatct tcaatatcag ccgactggtc ttgatatcct ttacaatgta attccagaag
    31021 ggattgacaa gaatggtcgt tatcggcgta atggcaaaaa tcaatcccaa gtcggaatcg
    31081 gaaattccct ttgatacaaa ataaatcgac atgaaaggat agaaaaaggc atcaccaaaa
    31141 taacggataa aataaaacaa cttgaatttg aatgtctccg acattttcat gacgttttcc
    31201 tcctctcgtt tggcatagtt gatattatac attcacggta tgcaatttgc aacgaagacg
    31261 attattattt tctgccgtct gaaaagatgt tacttttctt tcggtttcac aatggaaact
    31321 gaaaggaaag aaatgtattt tcataaatga acgttctcta ttttcagaaa acgatacctt
    31381 ttcgtctctt ttcccaaatt tgaataatat acgtataaaa tattttttta aacttcaacc
    31441 gtcatgattt cgcaccatgg cgctgttcct tggcaattgg caaaaaattt acaatatttt
    31501 cattttcaaa ataatattga caaaatcatt tttgccaatt attatcataa atacctaaac
    31561 aaaaagggtg ccgtttggag gggaccgttg tgatcaagca aatcgttaaa agagaccaat
    31621 cagtagtacc gttcagaaga gaaaaaatcg ttcttgccat ttttaaggca gcgagttcgg
    31681 ttggcggctc cgattttgcc aaatcggaag agttggccgc cgaagtcgtt ggcatggcag
    31741 aagctaaata tccaaacggt attgccgatg tcgaagggat ccaggatctc gtcgaaaaag
    31801 tcttgattga aaacggccac gccaaaactg ccaaagcatt catactttac cgggaaaaac
    31861 gtcgggcatc cagagaatca aatgctctaa tcggtgccac aatcgatatg tttacggaat
    31921 atttgaacga ccgggattgg caaatcaacg agaatgccaa tacccagaaa tcaataaacg
    31981 gactgaataa ttatgttcgt gaagaattca cgaagaacta ttggctgcac gaaatatatc
    32041 ctgaagatat ccgcaactcc catattcaag gagatatcca tatccatgat ctggggtttt
    32101 tcggtcctta ttgtgctggt tgggatttaa gacaaataat caccaacggg tttggcggag
    32161 tttccaacaa agtcgaatca agccctccca aacatcttcg ttccttgctg gggcaaatca
    32221 tcaactccac ttttacaact cagggagaaa ccgcaggcgc ccaagcttgg tcttctttcg
    32281 acacatatct ggctccgtat gtcagatacg acaatcttga ttacaaacag gttcgtcaag
    32341 ccatccagga attcatattc aatctcaacg ttccgaccag agtcggattc caatgtccgt
    32401 tctccaatct cacgttcgat atcataccac caaagaccat gagagaccaa aacgcgattt
    32461 acgccggtca gcttatgccg gaaacctacg gcgatttcca agccgagatg gacaccatca
    32521 atctggcatt ttgcgatgtc atgatggaag gcgatgccaa gggacgggtt ttcactttcc
    32581 cgattcccac gatcaacgtc accaaggatt tcaattggaa cagtccggtc gttgagaaat
    32641 acatggaaat aaccgctaaa tacggaattc cttatttctc caattacatc aattccgatc
    32701 tttctccgga agacgcccta tcaatgtgtt gtcgtctcag actcaacaca tcggaactcc
    32761 gcaaacgcgg cggtggtctt ttcggttcca atcctctgac cggttctatc ggcgttgtca
    32821 ccgtcaatct tccaagactc gcctatcttt cccgttccga atctgaattt ttcacaaggc
    32881 tttgggaaac catgaatatc gccaagaaaa gtctggaaat caaacgaaat atcatcgagg
    32941 aacaaacaga taacggactc tatccgtaca gccgcaatta cctcaaagat gtcaaactga
    33001 aaagcggatc ttactggttt aaccatttca ataccatcgg cttgatcggc atgaatgaag
    33061 ctgcaatgaa cctttatgga aaagaagtcg atctgacaac cgaaaaaggg caagacttcg
    33121 ctttcagaac catcgaatac atgcgcgatg tcattaagga tatccaagaa gaaaccggtc
    33181 attattacaa tctggaagcg acgccggccg aaggaaccag ttatcgcctt gccaaacatg
    33241 acaaagaaag attcccggac atcatcactg ccggcaaaga cacgccatat tacaccaatt
    33301 ccagccagct tccggtagga ttcaccgatg acatctttga aactctcgat ttgcaggaca
    33361 aactccagtc gatgtatacc ggcggcaccg ttctccatct atatctcggc gaaagagtca
    33421 aagatacgga aacaacgaag aatctgatcc gcaaggtatt tgaaaattac aaacttccat
    33481 atatctcgct cactccgacc ttctccgtat gtaatgacca cggatacatt tccggcgaac
    33541 acttcaactg cccgacttgc ggcaaaccgg ccgaggtcta cacaagagtc gtcggatatc
    33601 ttcgcccggt tcaaaacttc aacaaaggaa aacgagccga atatgcggat cgcgtgaaat
    33661 acgtaatccg aaacgaaaaa acggcggaag aggatcgcaa agcatgatat ttgccggaat
    33721 cgccaaaacg tcattggtgg atttccccgg aaaaatagcc accgtgctct tcacaccagg
    33781 atgcgactac gactgtttct attgtcacaa ccgatcgttg atcgaggatt tccaggacat
    33841 tgttcccaat gaagaaatcg aagaattctt gaccaaaaga gttggcctgg tcgacggcgt
    33901 agtgatcacc ggaggcgaac ccacactaca tagcgatctc atatcttaca tgatcaaact
    33961 caaaggaatg ggttatctaa ccaaactcga taccaacgga tcgcatccgg agattgtcga
    34021 aaaatgcatt gaggccaaag cagtagacta ctatgccgtc gattacaaag ctcccaagga
    34081 cagatacaag gaaatatgcc ggggcgaaag cgatgccgac gttgtgcttc agactatctc
    34141 cctgctttta tcggccaatc aggatttcga agtaaggacc accgtcatcc cgcaattgac
    34201 gctggaggat cttatcacaa tggcggaaga gttgccattg gtgccaaaat acactttgaa
    34261 tccttataaa aagccgcttc attttcgcga agaggaccgt gcacttatcg aagcgcagcc
    34321 atattcggaa aaagacatcg cggcttttgc ggaaactctc aaattgtatc aaccaaacac
    34381 agttctggta ttttaaagcg gaaaacaatc cgcttttttt cattaaaaaa accgacgtcg
    34441 tgacgtcggt atagcaatcg gtggtggaga gagctggttt cgaaccagcg aacccagtgg
    34501 gagcagattt acagtctgcc gcgtttagcc tcttcgctat ctctccgtcg ggctccgatt
    34561 tgctattaca ttatatcaaa tcaaaatata aaaatcaaga taaaaaaacc taagttccaa
    34621 atacgaaaac acaactgcgt attgacgtga tataataaca atgatggtaa attttcgagg
    34681 tggtaaaaac atgaagatag ctttgatagc tcacgacaaa atgaaacaag aaataattga
    34741 ttttgtaaca aaatatcgcg atgtcctgtc aaaacatgaa ttgttcgcga ccggcaccac
    34801 gggacataga attattgaag caaccggttt gaatatcaaa tgctttcgtt ccggtccgct
    34861 aggtggcgat caagaagtgg gggcaagaat cgccaatggt gaaatcgatc tgattttctt
    34921 cttccgcgat ccattgacag cacagcccca cgagcctgat gtttccgctc ttttaaggct
    34981 ttgcgacgta taccgggtac cattggccac aaaccgatcc accgccgaac taatcgctca
    35041 cgcgataaaa tgattttatg aaaagcgcgc ccttcataag gcgcgcattt ttttattttg
    35101 aaaacacaac aagcggcaca agcatctctt ctgatgtcaa tccggcatgt tgccctttca
    35161 tgaagaaatc cgtttggaaa aacacgaagt acatgtcgct gatggcaatc gccatatagt
    35221 cgcctaagaa ttcccgaaag cggggatgct cgctggtgta tccgaaaaga ttcatcttca
    35281 aaatttcttc ggttttgtaa aggatatatt cgttacggaa gtttgaaaga aactcccttt
    35341 cgaaatcgtc cttttttcct tccttcaccc aaaacgtcac agctcgtgat tctatggacg
    35401 gtttcttttc taaaaggctc catatctttt cgtattcata aagtttgatc ggcttcaccg
    35461 caatttgccc gtggtcggca atcattatga caatgctatc agacggtagt ttttctttaa
    35521 tggccgaata tcctttgtcg acggcatcta acgtatcggc agcttcctgg ctatcgggac
    35581 cgaactcgtg catgacggaa tccaactgat cccagtagac ataagcgaaa tgtcgtcccg
    35641 gcttttcaaa ctccgtattt aaagtttcca attgcttggc gaaagtatca tgttccggtt
    35701 cgcggaaagc cggaaatatt tctttagtcg tcaagtccgg attcttggct ttaaaaatgt
    35761 cgtatatttc tgtgacggga acataggtat tcgcaaccgg aatatcgatt tttgcggatt
    35821 catcatagta atctttgttt ttgaagaata cgacattccg atcaatatcc cggacatatt
    35881 gcatccagcc aatccatccg gtgctgatcg gcggttttcc ggtcagtacg gaagttgttg
    35941 cggcgacagt agtcggtgga aataccgaag atatagtcgc aactttgtcg cttgcaatat
    36001 tttttgcctg ttcactatga ttttgcaaag tcgattcccc catggcatcg acaagcacaa
    36061 gggcaatatt gcgaaattcc ttgttagcta aaatattatc aagcaacggt aaagacggag
    36121 caaacgtttc gcacccgtaa tactttagaa gcgaattaga cacattgaga atcgtgtttt
    36181 tgtaatcggg ataaaatagc gatttcatgc gatttcctcc tcttgatcat ttatgattct
    36241 ggacgttctt atctttggca agaaacggac tttcgaatac aagaacatcc cgacaaacac
    36301 aatcaggata ttgcttatta gcattgaata ccaaatgcct gacggaccga gatcggtgaa
    36361 gttgatgacc actaatatca gcggcaagcg aatgacccac aaacgaatga ccgccatcag
    36421 gaaggagaat ttagtttctc cgcttccctg gaaggtcgac aggtaagttt ggaatattgc
    36481 catcaaaggc aatcctatgt tgagatagaa agtatactga gcggcaagcg cgatggtctc
    36541 gggatcatgg ttgaacaaac tgacgaagaa atcccggaaa ggaattatca gcatggcgcc
    36601 aacgctcatc atgagaatac tgatgatcat cccggaatga aaagacttct tagcgcgcgg
    36661 tatgtttccg gccccgacat tttgagctat ataaatggca acgatcgaac tgacggcgga
    36721 tgccgggaac atcaccagcg aattgattcg gttgccgata tagaaagcag ccgatacttc
    36781 tgtcccgaat gaataaatca gggaattcat gatcacgaag cccaaagcct ggatcgattg
    36841 cccggtggac gccggaacag ctatttttat tatgtcattg ataatgaaac gcttgacttc
    36901 gtgtatgcgc caatggactt ggattccttt tctcgcatac acaaaaagca aaaggatgaa
    36961 gggcagggcc agccattgtg ctataagcgt cgacaatccg gcgccaacga tacccatgtt
    37021 ccacacaaga atcattatcg gagtcaaaac aagattcacg acaatactga aggcgctaat
    37081 aatgaccggt gtgacagtat cgcctgtcgc ctgtctaaca gcctggaaag caaaagccag
    37141 aaacaacacc ggaaactcaa aactgcggat cctcatataa gtaaccgcat acgagtaatc
    37201 ggatccttgt gctcccatga accgaaaaat caacggtgtc atgaaataca cgaagatcgt
    37261 gaagatcgct ccaagaataa ccgacaacac aactgtattg agagcgtatt tttccgcatt
    37321 ggccttgtcc ttgttgccga tatactgagc tattaaagaa tttccagcca ccgacaaccc
    37381 cattccaaag gacagaaaaa cgaaatatac cggccaggac aaagcaactg cgctctgcat
    37441 cgatgtagaa acggaaaatc catccacatc gggaacctgt ccgagaaata ttccatccac
    37501 cacatcgtgt agagtcttca gaatattaac aagaaaaacc ggcacactaa gagacaatag
    37561 gcctttccag attggatttc cgtataatat tttatgtgtc tttttttcta tctcgggttt
    37621 catcgtgcaa ttgcctttcc ataaaaaaga agcgattttt gatcctttgt cctaatgctt
    37681 cctaagcgct cgatacatta tacaatgatg gtataccatt gtcaacaaac ccgcatcgtt
    37741 aggtaaatga taatcatttt cccttgcgaa acaaaactat tctgtctttt ggcccatttg
    37801 gtattctttc gtattcgctt tcttccaaaa ccgaaaatcc atcaaaaata ttcttccatt
    37861 cttctatcgt gcgattcaat ccgcgcatta ttccgttgat ggaatatccg ttttcttcga
    37921 tttccaccat attcaatttc tttatgagtt cgtcggtcaa ataaaagttc aatttcaaaa
    37981 gaaaaagacc gccgggtttg agaagcctta caatttccga tgcaatctta tcagatgttt
    38041 ccgatggtac aacatcaaga acgttggaac atatgatccc atcaaaactt tcagctcgcc
    38101 aatccgaaag caagctgtga tcgcccaaaa aacattcgat attatgcaac ccggaaagat
    38161 tgacgactcc gtttacatat ttaaccgcat tttccgacgg atcgattcca attatattct
    38221 taaccttcgt tcccgctaaa gcggctgtaa acacgccata tcctgagccc ataccaacat
    38281 ccaatatgtt ttcgcattga ttgcccagtt gtttgaaata tccgcttaac acgtcctcgg
    38341 gaatatcggt gatttccact ttttctggcg tcaacttttc aaaattcttg tcccagaaag
    38401 caattagatc gcggttcgtt tttcttacat ccattatttt cctccgaata tgtgaaatat
    38461 atgcaataaa aattatatca tcatcaatgg ccatattcac ttgcaggaat aaaaaaagac
    38521 ggtcgacaac cgcctctttt cataacagaa gttttccttc tgttagatgc gctatggtgc
    38581 cgaaaacagg aattgaaccc gtaacctact gattacaagt cagttgctct accaattgag
    38641 ctatttcggc atggtggagg ttgacggtct cgaaccgccg accttctgct tgtaaggcag
    38701 acgctctccc agctgagcta aacctccgtg cgtgtttgcc agcgttaggc gatcaaagac
    38761 gcatgattcc gatttgacgc ttgcattaca attttactat ttacccaaat caaaagtcaa
    38821 ggaaaaaaag caaaagaaac cgatgtgcct caagacagag tcggaatgtc caaatggaat
    38881 gtttttctaa actcctccaa aaacgccgca actaccaata aatcggcgct cccgccaaaa
    38941 ctcagattat gttcgatgca gtaatctgtc atttttttga tattgccttc cgaaaaatcc
    39001 gtcagatcct tgaacatgtt ctggtatttg tgataatcct ctatggtaac gctgcgtttc
    39061 agaaaaacag tgtcttcaca tgtgcctatc aaatacacca gggtttttgt cagaacaagc
    39121 ggatcataaa tatcatttag cgttttcaaa gcctccgata caagcggaaa acccatttcc
    39181 gcttctttac gtgccccggc aatgccgtac ttctggtaag ctattttacc aaacgtatcg
    39241 tgtcccgtct caagttcttt cacaagtccg gacgacagtt cctcggctcg aataaaaatg
    39301 cgctcgaatt gacccttacc gtgcaatacc atggcagtcg aagcaatgat tatccccaaa
    39361 ctgaaaatca atcccttgta tgcgttgact ccgtttgtgg ctctaaacat cgctttttct
    39421 gcttcttgac cgatttttct gatttccgga aaaatattct tttccacatc cacccgcaaa
    39481 gctacgataa acatcttggt gagatacggt atgatggcgt ctttcgcctt cagcatcttg
    39541 gcgtaatcca tatccctgtg cgatcccgac gatgttggcg taactagccc aaatttagga
    39601 tccaggttga gttccgccat gatagccttg tcaatcacgt cttgtaaaag tgtcatgaac
    39661 ttatcgtcga atatttcctg gtatatgcgc aacacttcgt tttgatcgtg ctttttggct
    39721 ctgacgcaat ttacagcata atcatcgcaa attacgcatt ttcgcaaaat gtcacgatgc
    39781 aaggttcctt ctttagtgtg tacatcgata tccaaaagcc gtccgacttc ttcggtattt
    39841 tcaaaccatt cagcttcctt tttcaagtct ttcgccgaca cgttgtcgat aaaacggaat
    39901 attgtcggtc catcggctcc gtccacgaac aaagaattgt tttgttgttc cggataaaat
    39961 agcttgctga aatgccggat caaaacgtat gctccgggta gtcttttatc cggtcccgga
    40021 atgtttgccc taatcgtcac aaccacatca cgatccctga gaaaaagagc aatcatgttt
    40081 gctctttttt cgcgatcgtt cagaatgtct tcaggctttg tttccatact ttttctccac
    40141 tatttccata aaagtgtttc tggcggcatc agggacaagc atcgacgctt cccttggttt
    40201 gccctccaaa atcagttttc tgacccgcga agcgctaatc ggttcattat cctgttgaaa
    40261 tctttctcta cgaacaagcg tatcgcccag atatcttttt aaaagattgt tataggttac
    40321 catgaccggt tctgtttccc cgccgacata gcgcttgcgg atattcagta tccgaatgaa
    40381 ataatcccgg aagatcatag catcaagttt tgcatgttct ttttcggctt cgtcgatgct
    40441 tttcaaaaaa tagcttggaa aagtcagaga tgatacaaca tatccggtag acggtataac
    40501 catgatattc tcatatccgg ccaatgcttt attaatcaga agcaatcttt ccggatatgt
    40561 aaaaaatgat ttttcctctt caacgaccaa aaccagaaat atctcgtggt ttgcggccga
    40621 atcaataatg agttggaaat gtcctttggt catcggattg cagttgacga cgatggcgcc
    40681 gatatcagct ttgtcaagac tgcgtttgaa tctttcctca atccgttttt tcatcctgtt
    40741 tattgtcgtt tcgatattgc cgtcaccgcc ctcaaggacg gcaactttat cggttttttc
    40801 gataaaagaa aatcccatgc tttcaaaaat agataagtat tcggctttgg tataaacctg
    40861 ataataaaag attttctccg ctctcatcga agccaataat tcagtcagca gtaaggatgc
    40921 cagattttct ccccggtgtt ccttggaaac ggcgattgct ttaatcagat tctttgatct
    40981 tgatccggtc gcgatgattt cgccctggtc ttcaatataa aatgattcat tgatatcatc
    41041 gtcatacttc aatccgaagg cgtcaagaaa aatccgcagg cggttgaatt cttctctgat
    41101 cagagctttc ttgacaatca ccactgtttc acctcgtctt atacagactg tctataaccg
    41161 ttccgtcacg atattccacg taaccgattg tttttgcggg cttggcaata tcagcgggaa
    41221 ctccgcatat cttatgggac aattccatca gttccctgat atcaatcaag ggcaatccgg
    41281 aatcatttag tttctctaaa atgtcttttc tcaaaggatt gattgcgata ccccgttcag
    41341 tgaccagaat gtccacatct tctcccggag tcgtcacggt cgtcacatgt tccttgataa
    41401 tgggaatcct ggctttggtc aatgccgatg tgataatagt cgttttcgct ccgtgggcgg
    41461 tatcggaatg tcctccggat ccgccaatga tgttcccaaa agaatcgctt gtcacattga
    41521 cattgaaatc aagatcgatt tcggtcgctc cgagaattac ggcatcgagt ttgtcaacga
    41581 tcgcatcttt ataaaacgga tttgcatatt gtgaagcgga aataccgata tgtcctgaat
    41641 tttcccgata agatctgacg gcttccaaat caaaacactg aacatcataa atattcttaa
    41701 atattcccga ttccagcatt tcgacatgat atccggttat gccgccggac aggaaactac
    41761 cggttgcttt tttgcttcgc atttttttct cgatctgttc cataaccgca agcgatgttc
    41821 ctcccgctcc agtctgcagg gaagtgcctg tttccaaaag gccgagcttg tcaagcaaca
    41881 aggctgtgat attggcgatc ttcaatccga tcggatctct cgttattttt gtcgttcccg
    41941 agactatgcc ttcgggatct ccgattgaag gaactttgac aacataatct acataacggt
    42001 gatcaaattg gtattcatcc aatttttcaa caatattatc ggtcaccaaa actacttttt
    42061 tagcatgata aagatcgctt atgccatatc ctaaggcgcc acacgcgctc tttccgaccg
    42121 ctccagaccc attcccggat gaatctacgg tcgggcagga tagaaaagcc acatcgattg
    42181 ggagctcgcc ttcttcgatc gctctggcac ggcccccgtg tgtttgcatc acaagatgtc
    42241 ctttgagaaa tccttcctga atggcgattg ccaccgggcc gttcagataa ctcgtatgaa
    42301 tatcacgaat ccgcccggaa cggataagag gtgccaacat atattccgga aaaatcgaac
    42361 ttggcgccag acttatgtcg cgaagatcgt gtttctctat cagcttggca acctcattga
    42421 tcacatggtc accgcttctt aagtgatgat gaaaagacaa tctcattccc ggcatgattg
    42481 aaagtacgtc aaatgccgct tccagactgt tgagaaatac aacattttcc gccggcttct
    42541 tttcctttgc ctcatgatag attctttcag aaaaattagc agcggatata aatggcttat
    42601 ttccctccgg aatttccctt cccaagctat tcttcatcgt tatcatccca cgctttgcat
    42661 gcccgggctt gctcgcggat tgccaatgcg cgggctataa ccggtttatc tatcatcttc
    42721 ccgtccaaac tgaaaacgcc gaatcctttt ttatctgcat cttttttggc ttccattact
    42781 cttttggccc aggttatttt ttcttcggtg ggtgaaaata cgttgttgat gataccgacc
    42841 tggcgcggat gaatggcggc cttgccggtg aatcccagat tcatagctgt caatgcgtca
    42901 tcctggagac cgagatcatc gttgacatca gtgaaaggag tatcgatggc cagaatgtcg
    42961 tatgctttgc atataatcgc cagttttgca cgcggatatg ccagctcatc gccttttttt
    43021 gttctggtta ttcccatatc ggctgtaaaa tcttccgctc ccaacatcat ggcttccacc
    43081 cggggacaaa aggcaatgtt tttcgcttca acaagcgaag c
//
LOCUS       DAOPJL010000007        41621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_982_length_41621_cov_8.418467, whole genome shotgun sequence.
ACCESSION   DAOPJL010000007 DAOPJL010000000
VERSION     DAOPJL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 41621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41621
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_41621_cov_8.418467"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PLP02_01795"
     CDS             complement(<1..876)
                     /locus_tag="PLP02_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPF42227.1"
                     /translation="MDFVKKEKPYFTANDFYRARFGHKIAKIPLNGGFTCPNRDGTLS
                     WDGCIFCSEKGSGDFAGDPGESLGVQYARSLEVLHKKWPGCLTIPYFQANTNTYAPLS
                     RLKKLYEEAINLSDEVVGLSIATRPDVLPDDVVEYLGELRQRTFLTVELGLQSIHAST
                     ASFINRQHSLESFVDAVAKLRKKDIHVICHIINGLPGETPDMMLDTIRFLNKQDIQGI
                     KIHMLYVMKNTRLADLYEKGEITMMSRSDYIETTADQIELLRPDIIIYRLTGDCPRKE
                     LVAPLWSLKKFTVTND"
     gene            973..1818
                     /locus_tag="PLP02_01800"
     CDS             973..1818
                     /locus_tag="PLP02_01800"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HPF42228.1"
                     /translation="MFNIVTDSILHPDRLVNYRNRSGWFVFLYLLILAIFVSAGSIVY
                     SIKNRGEINLTSEATGCTLVNGFLVCEGLSHDNSVAFNAYDYSVYFFDEDGSVDLLDV
                     DDADSLIVFQGQSMSIYIGTSMIMQTPAFAGMSGDDAFDYFMRTFVNSFSVMIVVAAI
                     ITNTALLAVISLIAMIVFSRLIKYISFGKIYKLVVFASTPVALILVFNNLLSLPTILF
                     IILMALAYRSIFLLQRELYIYTMIHNSQQQGGSGQNMGMPDDKMENDDQDLDDDGNDN
                     DDPEE"
     misc_binding    1867..2066
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2098..4518
                     /gene="leuS"
                     /locus_tag="PLP02_01805"
     CDS             2098..4518
                     /gene="leuS"
                     /locus_tag="PLP02_01805"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPF42229.1"
                     /translation="MSEFIPSFNHTEIEKKWQDRWYASDLFHAVDFDKRPKYYALVEF
                     PYPSGIGMHVGHFRAYMSLETIVRKRRMEGYNVLFPIGFDAFGLPTENYAIQNNIHPR
                     IVTDQNIKTFTKQLREAGFSFDLDRIVDTTDPEYYKFTQWIFLKMYEHGLVYRSKTYV
                     NFCPDCKVVLSNEESQGGTCDRCGTEVVQKEKEVWFLKITAYAEKLLEGLKILESPER
                     IKTEQEKWIGRSDGAFISFPIADSADSLEVFTTRPDTIYGVTFMVIAPEHPAIKKYED
                     RIKNMDAIKDYAENSKRKSEFERVQLVKDKTGLKIVGLEAVNPITKEKIPVFIADYVM
                     MTYGTGAIMAVPGHDQRDYEFATKYSQKIVEVIKGGDLSKEAYTDIEDGVLVNSGILD
                     GLKVKEAIAKMIAYLGELGCGKKGVQFKMKDWAFNRQRYWGEPIPLVYCDDCGIVPVP
                     YEDLPVTLPMVDSFIPTDTGESPLANIKEFVECTCPKCGKTARRETDTMPQWAGSSWY
                     FLRYIDPHDKTVFASRDKLDYWGQVDWYNGGMEHVTRHLIYSRFWNLFLNDIDEVPFA
                     EPYCKRTAQGLILGSDGEKMSKSKGNSVNPQDIINEYGADTLRTYILFIGDYELPTPW
                     NDNGIKGCRRFLDKIWRAFPKVNSVPGFSIESEILMNQTIKGVSDDYESMKYNTAIAK
                     MMTLMNELGQKEEITRGEYETLLKLLYPVAPHITEELFEKLGKGHWLDKESWPAWNEE
                     KLAEDKIEIVVSVNGKVRDKLTVPVGTGRDKLEKLALELPKIREITKDNQVRKVIVVE
                     GKLVNLVI"
     gene            4563..5777
                     /locus_tag="PLP02_01810"
     CDS             4563..5777
                     /locus_tag="PLP02_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPF42230.1"
                     /translation="MKEYLHEKNREAKEKLKAIANGMCNRCFSHNLANDCHDKLYCPD
                     CLVTEDDYIFRYERELPYCDPFIRPDLVLTKRQSDASRFLLELLSDRKSGNLFAVCGA
                     GKTEMTYLVASEAIRRGMSVCYAIPRREVVRELAKRLRRDFKGIMVGEMHMEKKADEG
                     CQIVVSTINQLINYYHEFGLVILDEADAFPYSGNEYLERLVKKAALPDAVFINMSATM
                     TKGGIIDTYIVPERFHGFRLDKPRFVKTGNILETLKNGKLPARFESKLNLWLDSGKRV
                     FVFVPTVELGKKVAKIMKNGKLPVDFLYAGNPARRRILNDFYMQKFAILVTTTVMERG
                     VTYDDVQVAVLAADHPIFNKACLVQISGRAGRFKSHPSGEVVFYSETLTGQMMSCRRE
                     IARMNRLRNNSS"
     gene            5774..6172
                     /locus_tag="PLP02_01815"
     CDS             5774..6172
                     /locus_tag="PLP02_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42231.1"
                     /translation="MICAICRKTFVAELEFSTLFQPPILCGDCLTLRKAEPIMEAIPT
                     DNGLIEYYYLCEQNDLDTFMYSRSSHFIEKALEMAICRYNEIGFVIILEPDDFTTLES
                     WWPFVKETGTMIFFSFFRYDFLTEKKDFCF"
     gene            6254..6790
                     /gene="raiA"
                     /locus_tag="PLP02_01820"
     CDS             6254..6790
                     /gene="raiA"
                     /locus_tag="PLP02_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009677046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPF42232.1"
                     /translation="MKLDVRGKDGFEITDSIRSYFEKKLKKVEKMFSTELEAFAVCKI
                     YKGMSKVEITIPIKFVTIRAEVLDKDMYAAIDKAVDKLEAQIRKNKYKLNRSLQEKTG
                     LKETFKSDELDIDLLEKELISPIRKKRIQLDMLSTDEAITQMELLGHDFFIYRNEDKE
                     VCVLYLRDDGKYGLIETY"
     gene            6895..9387
                     /gene="secA"
                     /locus_tag="PLP02_01825"
     CDS             6895..9387
                     /gene="secA"
                     /locus_tag="PLP02_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014589130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPF42233.1"
                     /translation="MPKLFDPNRKILKRLEKTAEKVLALEDEFKQLSDDELKAKTDSF
                     RERYKAGETLDDLLVEAFATVREASTRVTGLTPFKVQVMGAIVIHEGNISEMKTGEGK
                     TLTAVMPAYLNAIPGEGVHIVTVNEYLAGREVSGEIGELFRFLGLTVGLNVRDITPEE
                     KRAVYNCDIVYSTNNELGFDYLRDHMVIYKENIVQRPLNFAIIDEVDSILIDEARTPL
                     IISGGEKSTAEMYKQANYFAIRLKEEEDFAIDIKEKTVNLTEEGIKKAEAFFNLENLY
                     DISNVVLLHHINNALKANFTMSRDVDYVVHEGKVIIVDPFTGRLMHGRQFSEGLHQAL
                     EAKEAVEIKKETTIMATITFQNFFRMYKKLSGMTGTAKTEEEEFTNIYNMYVVEIPTN
                     MPVIRIDDNDLIFATMQAKYKALADEIEKRHNLGQPMLVGTISIESSELLSSLLKRKH
                     IKHDVLNAKQHEREADIVAQAGQMGAVTIATNMAGRGTDIKLGPGVKELGGLAVIGTE
                     RHESRRIDNQLRGRSGRQGDPGYSRFFLSGDDDLLRRFGGDRFKSMISMITTQKGSEA
                     ELPLDYKMFSKLVMKAQHQIEGNNFDRRKTVLQYDEVMRRQREIIYKQRTDILFLETI
                     EDTVNQMIESVLSSLINHYAEDRDAMYEALNKYFQKDMIDRDILKTEENATSYVFDQY
                     KKEIENKKSLTGDKIYNDFLKAVTLRVVDTYWIQHIDAMSELRQAVSLQSYGQNNPFR
                     EYQDTGFQMFEEMVFNIQSDVTRFVLKAQVRQNTERVQVAKPTYASSGKEDENKKKKP
                     VVKSDTTKVGRNDPCPCGSGKKYKYCHGRNAD"
     gene            9406..10506
                     /gene="prfB"
                     /locus_tag="PLP02_01830"
     CDS             9406..10506
                     /gene="prfB"
                     /locus_tag="PLP02_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139279059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPF42234.1"
                     /translation="MNQLELKTKINELTEWLEELGRTVDEKDRQEKIRDIEAKMLAPD
                     FWSNAEKAQKVIREQNDHKSVIAEYHRLCDILETLNMTYELVSEGEEMDLGEAEELIR
                     EFEEKDDALTIRLLLDKPYDKLNAILEFHPGAGGTESQDWAQMLFRMYTRWAEQNGYK
                     VTVLDYLDGEEAGLKSATILVAGPNAYGYLKAESGVHRLVRISPFDASARRHTSFASV
                     SVVPEIDDSIDVDIDEQDIRVDVYRSSGNGGQGVNTTDSAVRITHLPTKLVVTCQNER
                     SQIQNRETALRVLKGKLYQLELEKKQKELDSLSTTNVQNAFGSQIRSYVFQPYTMVKD
                     HRTSFETAAIGKVMDGDLNGFINAYLRQTKSR"
     gene            10519..11460
                     /locus_tag="PLP02_01835"
     CDS             10519..11460
                     /locus_tag="PLP02_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPF42235.1"
                     /translation="MAKHRSLRRIGRFEQYAFLTLGVLIMASAYYFFVIPSGIVTGGV
                     TGLSMILQRYLPNIPISAFAFCFDMIFLALCFFFLGKGEFVRSIYGSILFPGFLALFE
                     NTVPNPTFGPNDLLLVALYAGGLIGIGFAIVVRYGGTTGGTDIPIKILKKNSRLSLAT
                     SVYLIDGSVILLGAITSQSGIGQGFIDALYALVIVILSGKVSDSFLMGLEGKKAVHVV
                     TAKPKEVKAAVFALFSRGMTEMRVQGGYTETDKSLLVMVIQNNEYLFVRNIITRTDPA
                     AFVYVTPASEINGEWSPGEEVYIGNARNRQQSQEKKQ"
     gene            11420..12223
                     /locus_tag="PLP02_01840"
     CDS             11420..12223
                     /locus_tag="PLP02_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPF42236.1"
                     /translation="MPATVNSLKKKNNKYIVSISTPLGKDKEYVVSEDLVVEYRLVKS
                     KILSPDDYAIFLDVCEFDRFFQKAKHWLLFQPRSLSQTREYLEKAGASDSLVEKTIAK
                     LREKHLLDDDDFACRYALILLEDKRFGQRRIAFELAKKGFDPESIKLALFEINHDTIY
                     ENMNYLFEKKLPTLGKSSLNAAILTMKHHLGQKGYEASDIQDIMAKRKGEFNQFIDEK
                     KQIEKDYRLVKAKYLRKDLPEAKRRDLTIQALLRKGYSYELIKETVERG"
     gene            12227..14101
                     /gene="glgB"
                     /locus_tag="PLP02_01845"
     CDS             12227..14101
                     /gene="glgB"
                     /locus_tag="PLP02_01845"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPF42237.1"
                     /translation="MDYKMTELDLYLFNEGKLKEAYKHFGSHLVYDEDGKFLGTRFTV
                     YAPHARIVSIVGDFNMWDSRTHVMTKTDQLGIFSLFLPDVGEWVRYKYCIVTSFGQTI
                     FKADPYAFFSDNRPETSSKVYDIDHFDWSDRDYLENRKKGNPFMAPMSIYEVHLGSWM
                     TKPDGSFHKYNELVDHLLPYVKSHGFTHIEIMPIVEHPLDESWGYQGTGYYSATSRYG
                     VPKDFMYFVDMCHKEGIGVIMDWVPGHICKDPHGLYMFDGEPLYEYSDYSIRENEVWG
                     TVNLDLGKGATKSFLISNAFFWIRYFHVDGFRIDAVSNILYYLGNSAIGTNYPAIEFL
                     KNLSYAIKELDHSVLLFAEDSTTFPKLTTSVIDGGVGFDYKWNMGWMNDTLKYFEKDP
                     IYRKYHHDLITFGMVYAYSERFVLPLSHDEVVHGKKALVAKMPGDYWQKFANYRLLIG
                     LQFTHPGKKLLFMGGEFAQMHEWKDKEELDWFLLGYPMHEKAARFVRDMLLVYKQEKA
                     LYELDHDPRGFRWIDQTNRDQSIYIFLRQALDENEFVIILLNMTPNAYEHFKVGVPKA
                     GIYTEILNSDKEVYGGSNVYNGMPLVTIAEPNHGMTDSIDLKVAPLAMSVLKYIGNKN
                     "
     gene            14123..15247
                     /locus_tag="PLP02_01850"
     CDS             14123..15247
                     /locus_tag="PLP02_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPF42238.1"
                     /translation="MKKTIIAMILVGGAGSRLQEITRDLAKPAVSFGAKYRLIDFTLS
                     NIANSDIDCVGLVTQYEPYELMNYIGSGASWDLDVIDGGIHFLTPYTKKASILWQKGT
                     AHAIGRYLWFAQEFEADFVLVLSGDQIYKMDYRKLWEHHLNHNADLTIAATRVPKDEA
                     SRFGILSVDEESRVLAFSEKPEEPDGNLASMGLYLFNTDVLVDLLKDSSEESLDFGMN
                     VIPKSLKQKKIVSAYEFDGYWRDVGTVEALYKANMDMLDDPDFLGLNVSRNLPVYSKS
                     LNLPPHVVMDHAKIRRSVIADGSVVDGEVSHSCIGYECMVKAHAVIRDSVLLPGAVIG
                     KNVRLKNVVVNRNTVIPDGFVFEPESITLLDTTNLERAVK"
     gene            15249..17678
                     /locus_tag="PLP02_01855"
     CDS             15249..17678
                     /locus_tag="PLP02_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPF42239.1"
                     /translation="MPNLFLVCDASNIGAFSKLTRHRMPGALPFGAKYRLIDFTLSNC
                     KNSHITNVAIFPYGNYRSLSDHIGSGDRWDLNRRKDGIFILPPKNLNLTIEDSISFQR
                     MYEHIEYFLRSSQEYVIVTPSSLVWNIDYQVLLYSHLVSKADITEVLTADRKRIQTFV
                     LSKMLLLEYIRNYDTIPYRNLTEVIDYATNLKKNPYHFEQACFFINSPFELYEANMQL
                     LYPETRAQLFRKDRPIFSKETMSAPARYGDKAKVSRSIIASGALIEGEVKGSIIGRKA
                     YIAKGALIENSVIMNQCVIASGAHVEYALLDKETKVAENAEITGNLNQLFVSEKKQVV
                     VSGEDLRVVQITAECLPFVKTGGLADIVASLAENYSLLGVDSSVIMPLYPIIKEKYKM
                     FLEMRADQSIEYGSDKYHVTIYYYNNQNVNYYFIESFDFFDREKIYGYDDDGDRFAFF
                     SKAAIAFCDAFENQPDVIHIHDWHMGLVPLILKEDEKCRHIKTLLTIHNIEYQGLYNS
                     DIITKLGIKNYDIINKKINFLETGINTASKITTVSDTYREELHYEYYSRNLVDTINRR
                     DRDFYGILNGISTDVDPINDLTIQEKYNLMNVFSAKKTDKADLQKRMGLKQGNDYFIL
                     GMVTRIVEQKGFEILLPSLEDIIRNPMIQFVILGTGEERFIENLNQLKERYPDQVSLN
                     IGYDATEPGYIYAGADAFLMPSRYEPCGTGQMIAMRYGTIPIVRQTGGLNDTVEHFDQ
                     ITKRGNGFKFYNYDSRDLSFQINLAFHVFKDAPDDWKQLVINAMNTRFWPQDTAKAYV
                     ELYRTMLIENP"
     gene            17753..20173
                     /locus_tag="PLP02_01860"
     CDS             17753..20173
                     /locus_tag="PLP02_01860"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPF42240.1"
                     /translation="MTKSIFRSKDVFWKEFADRLSKTYMKDPDNASIREMFNVLGTLV
                     REEIAGDWKKTDEMLKKADCRKVHYFSIEFLLGRLMTNNLMSLGVREVVEDAFADAGI
                     SLSDIEEYESDPGLGNGGLGRLAACYLDSMASLGISGYGHCIRYRYGLFRQKIKNGYQ
                     EERPDNWLADGYVWEVRKDEEAVEVPFFGYVGFEDGKMVYHPSERIRAVPYDVPIIGN
                     HNGKVNYLRLWNAEPARKYPKDMSPFEYESQLERISGFLYPDDTTDDGKRLRLMQQYF
                     FSSAGINSICRQHKQRYGTLKNLAEMNVFHINDTHPTFIIPELMRIFLDEEGMEWEEA
                     WKITEKAVAYTNHTILAEALEKWPVSLFQPLLPRIYQLIEEINRRFCNDLLQKYGYGK
                     IEQINQMAIVSNGIIRMANLCVVASFSVNGVARLHTEILKKYVLKDFFDLYPDKFHNV
                     TNGITHRRWLLYANPELSELLYETIGDGFVTNAAKLGDFLKYASDKETIKRLFEIKRI
                     KKEALAARIYKEQGLTIDPDSIFDIQVKRLHEYKRQLMNAINIMYVYKQLKEDKEFRR
                     RYHPHTFIFGAKAAGSYYFAKKVIKLITTIAALVNADEETNKWLKVVFVENYNVSYAE
                     MIMPAADLSEQISTASKEASGTGNMKFMMNGAITIGTEDGANVEIHELVGDDNIVIFG
                     LSASEVEKLYSEKSYRPKELYDNDPVLKEILNHLIDGFFPDVDKNEFHDIFDKLVYED
                     PYFVLKDLPGYREAHMRANDLYKDKNRWAEMMVTNIAKSGGFSSDRSVEEYAETIWHV
                     NIAKGKHK"
     gene            20170..20811
                     /locus_tag="PLP02_01865"
     CDS             20170..20811
                     /locus_tag="PLP02_01865"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPF42241.1"
                     /translation="MKSRWIDLTHSISPDTMPYPGDPCLILEKNEKKGKDDFVLSRVT
                     TGMHLGTHVDSFSHLIKNGADIVSLPPDRFYGKAALVRVKQVGGLIKTQDVIYEWEKL
                     SDKPEILLLETGQANKFGKKEYYTGCPGFEPGFAKVLASFKVRLFGVDLPTVKYGVNG
                     NAAMHRDLFENEILIVESLANLDKLPVYFDFFALPLKIEGAEASLVRAMALAD"
     regulatory      20849..20966
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            21008..21610
                     /locus_tag="PLP02_01870"
     CDS             21008..21610
                     /locus_tag="PLP02_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPF42242.1"
                     /translation="MGNKRSTKYLIQVAILGAIAGILMIPELPIAFVAPTFYKLDLSE
                     VAVLIGTFSLGPGAGAMIEVIKILINLMVNGTETAYVGELGNLIIGLAFVVPAGFVYK
                     HNKTKKNAIVSLAVGGLMMVLFASIINYFVLIPAYSFAFGIDIDYIVSLGHAINENVN
                     GLLGFILVCVIPFNLIKAIVVSLIVFLVYKRISPLLKVSG"
     gene            21612..22037
                     /locus_tag="PLP02_01875"
     CDS             21612..22037
                     /locus_tag="PLP02_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42243.1"
                     /translation="MKRTLAFFLLMLFLPLFTGCSLTTTSTTVSTEPGTTYEYDDFSD
                     LLINSPEDQLTRPETDYYIYFYGEHCYLCQTIKQEALAYIASLETDKVYLVQVSSYDD
                     INEAIDFPGTPSIAHIVAHESVNVEKGESGVLGVLENLT"
     gene            complement(22050..22817)
                     /locus_tag="PLP02_01880"
     CDS             complement(22050..22817)
                     /locus_tag="PLP02_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42244.1"
                     /translation="MMEYKEAGYDFDRIKTARISKKNHSYAYVSLIFFIVFFSLFATF
                     LVLYLIGIPFEIDGIIRHPGDLPYQKFFFYFLGITGMVSFLMIATMIPNLLARANDYI
                     WIGSSAEGEKEIYVRDRYRETYLDESLMIMLDRKNQRTTYSESKTEIEAGRCSALFWE
                     ELKNVKLWIEKSRPGMHKIKYRLDRGNIRFIKTYVLYFDNFGNLFKYRETVYTVFHGN
                     SNLKSFCTYYVNDLNKYVHLPIDKEIENVVFQIKSGI"
     gene            22960..23679
                     /locus_tag="PLP02_01885"
     CDS             22960..23679
                     /locus_tag="PLP02_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPF42245.1"
                     /translation="MNTIKIDKYYRGMIAHRGLSGIETENTINAYIAAANRSYFGIEC
                     DVYASKDDKLILTHDDTLLRMGLLNVYIPGYKYEEIRRFTLIDRKTGHLSDTVTIPLL
                     KDYLAICMTYQKHAFIELKSNLTNAHVDQVIDEVRKYHRLEDTSIISFHDKYLVYLRK
                     NYPDMSLYLLSGTADEKTLAICEKNRLGLDAEWHAITETVVKRLHLTGLKVTVYTVDD
                     KNDAEKLIKMGVDYITTNILE"
     gene            23681..24577
                     /locus_tag="PLP02_01890"
     CDS             23681..24577
                     /locus_tag="PLP02_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPF42246.1"
                     /translation="MLKIVTVGACAGDYVGISLKRILKSDSNPGVVRLSSGGVARNIA
                     EDLFRLGADVLLISFAGSDRTGEFLAKEMKDLGISHKLLIKDKERSCCYLAIHDHDGN
                     LECGINDFSIFDTIGAKDFAPCLPDLDKADMIVLDCNLSHEALTYLIKRYKDKRIFID
                     GVSQAKIGRLSGLLEDIFLLKANMGELAALSGIDTKDDFAPAIDILFQKGLQNILLTS
                     GSGPIRLVGKDGIISINPQSPEKIVSTTGAGDALYAGLIRGLSLGLSKEKSLELGNRA
                     ACIVMACAAASSFLLSTDIIDM"
     gene            24591..25133
                     /locus_tag="PLP02_01895"
     CDS             24591..25133
                     /locus_tag="PLP02_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPF42247.1"
                     /translation="MKNPTIEAILGRRSVRSYLDKPIPEVDLKKIIECGIYAPSARNR
                     QSWHFTVITDKDTIDRMSELALEGMEKLGIVKDEGYHVFYHAPVVVVISSELEGYSEL
                     NCGCAMENMAIAAKALGIDSCIIGQTRYMFNQTNTADINLLLKIPEGYQHDCSICFGY
                     LSGDYPEPKPRKEGVVDYIR"
     gene            25204..26910
                     /locus_tag="PLP02_01900"
     CDS             25204..26910
                     /locus_tag="PLP02_01900"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPF42248.1"
                     /translation="MWKEIYQDWLDEKSLDPALKQDLEGKSDKELEDMFYTDLEFGTG
                     GMRGVLGAGTNRMNIYTIRRANTGLARYLEERYGRKNLSRGVAIAHDNRQMSKEFALE
                     SAKVLGYHGIKCYLFEDLRPTPELSYATRYLNAIAGIVVTASHNPPQYNGYKIYDEYG
                     CQYTPEYANRIISLVRDVKDLFHIGFMTEEELKKSGLLEYIGKKIDDSYLEMVKTVQI
                     HKELEKPLKIVFTPLHGTSAELGTRLLSECGYDIYPVREQMVHDPMFGTVKSPNPENP
                     AAFDMAKKLGMIVGADLLIATDPDADRLGIAVKKDNDYILMTGNQTGAVLLNYFLSEK
                     QKLGLLPKKGVVYNTIVTSDLGAKVARFFGMEVFSTLTGFKFIGEQARFLESTDKKFV
                     FGYEESYGYVANDKVRDKDSLQAMLLASEAATYYNELEKKTLYDKLEDLFKIYGYHYE
                     AMMNIEETGIEGKQKIARIMGFMQKYRETAFAGFRVDAIEDYKAGIGYKQNENYALTL
                     PEADVVKYIFSDGSWFVFRPSGTEPKLKIYIASVGKTAIEAKEKIEKLESAIRELLEG
                     IR"
     gene            26927..27124
                     /locus_tag="PLP02_01905"
     CDS             26927..27124
                     /locus_tag="PLP02_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42249.1"
                     /translation="MWKYIIAIGIVVLLVAIYIISYVLNQKTEIPEECRNLEDFSACS
                     TCTNQSCTILKKGIPELNDRK"
     gene            27111..27770
                     /locus_tag="PLP02_01910"
     CDS             27111..27770
                     /locus_tag="PLP02_01910"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPF42250.1"
                     /translation="MIGNDWDGILKPEFQKDYFRQLSEDIREEYRRYTCYPPIHDVFN
                     ALRLTSYENTKVLLLGQDPYHNPDQAMGLAFSVKEGVELPPSLENIFKELHDDIGCPV
                     PTSGDLTKWGKQGVLLLNAILSVRRYQALSHDKYGWTIFTDHIISLLNEKKTPVVFVL
                     WGSYAKSKKPLITNPIHFVIENVHPSPLSAFRGFFGSKPFSRINEFLESTNQDPVDFS
                     L"
     gene            27783..28538
                     /locus_tag="PLP02_01915"
     CDS             27783..28538
                     /locus_tag="PLP02_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HPF42251.1"
                     /translation="MHNKNIRNLVFTGLMVAIGVVLAQFLSIYIPNASEPIIKFGIGY
                     LPLIMASIIAGPVYGLMAGIAQDLLGFFIFGGPSGQTFFPGFTLNAALYGFLPGIIYR
                     IRKNSGTKLFFWLNLVLAVLLFLGSFWYFLHVDQVISSTLTDNQKLLLAGIGLIGTVF
                     LVVFDILVYKSKKYGDEGTKILFIISLMYVLTSLILTPIWLYIMNPGISFFVRIPLRI
                     VKMPIEVSFYVILVSVLLEALGITKRKLGPGSK"
     gene            complement(28577..30631)
                     /locus_tag="PLP02_01920"
     CDS             complement(28577..30631)
                     /locus_tag="PLP02_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HPF42252.1"
                     /translation="MKLKNILVSKIPSGNINYTVPDLWNAWNYSGEETHRLLSHELLV
                     NPYRFYSEVISSYILPKAKTNQNYLESLAKSSNTIPEKGTLGGEWVKKSVLYSTMIRA
                     SSAWDHDRSYSLDVSNLDEMKETGTFVKMLALLPLLQKLGVDTMYLLPISRYSRKEKK
                     GELGSPYGVASFYDIDPDLKDNLTGGEMTVGEEFQAFVEACHILGMRVVIDIIPRTNS
                     VESDLIRLHPDWFYWIETKDYDKYRVPYVTGLANTIPPEAKFMPDVYASEDVQRHISM
                     FRNDPYTENKELWNEIKDAEDIVEAIEKAYGLSVAPAFSDHINDVQPPWTDVTFFRMF
                     LDHPSDNVKYLKNKNIPPYILFDTIKSNLFPGKKPNMPLWETLSGIIPYYQEQFGIDG
                     ARIDMGHALPKELLKMIISKAREYDPDFAFIAEELNPENAAKAKASDYNIIIGNGFWM
                     EPRIWEKNLHKFVYGAKDVALPMFACAETHDTARIAGRDGGRELARMLTILNMLLPNL
                     VPFINSGQEIYEIQPMNTGVDCTDKDKFNLPKDDLFYGKLALFDKYAFHYTSEFRWEI
                     ADHMQGIKEIRKRWLSQIMDPNASLPIYFNEFDTPAIGVAYFDKKTGKCILIAANSNP
                     RQEIWCNASLAELRKKSGNENLSGNLVYSTYEMGRPYEDFTDDGRINLHLGGGEVKII
                     EF"
     gene            30795..32321
                     /gene="gpmI"
                     /locus_tag="PLP02_01925"
     CDS             30795..32321
                     /gene="gpmI"
                     /locus_tag="PLP02_01925"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPF42253.1"
                     /translation="MKKPVILMILDGMGIGEQNAGNAIYLAKKPHLDLLEQEFPTTQI
                     EASGEAVGLPEGQMGNSEVGHLNLGAGRVLYQSFTRLNVAIRDGSFKENPAYNKAFDH
                     VKKHHSKLHILGLLSDGGVHSHINHIKYMFQIAKEKGVERAYFHAFLDGRDTPPKSAL
                     TYVSDLESKMAELNFGSIATVGGRYYGMDRDKNWDRIQLAYDAMSFDKGPHFPSATAG
                     IQASYADNVTDEFVVPFICDDTGMIQSDDAIIFINFRPDRAIEISTAYSDPEACPKLN
                     TENHPENILYVSTMKYSDLVKGELAFPLISMKDTLGDYISDLGMKQLRIAETEKYAHV
                     TFFFDGGVDKQIEGAKRVLIPSPKVATYDLKPEMSAYEITDSVIAELDTGEYDIVVLN
                     FANGDMVGHTGVISAAIKAVETVDECVGRVVDKILEMDGIAFITADHGNCEKMLTEDG
                     KPFTAHTTNKVRLIVTKKGITLKDGGVLSDIAPSMLDLMDLKQPEAMTGHSLIIRDKE
                     "
     gene            32379..33665
                     /gene="eno"
                     /locus_tag="PLP02_01930"
     CDS             32379..33665
                     /gene="eno"
                     /locus_tag="PLP02_01930"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPF42254.1"
                     /translation="MPNITSINAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTG
                     EREAIELRDGDKSRYLGKGVLKAVRNVNEIIAPELIGYDVTMQTYIDKVMIDLDGTPN
                     KSKLGANAILGVSMACAKAAADWAGLPLYLYLGGFNAKVLPTPMMNIINGGSHADNNI
                     DFQEFMIMPIGAKDFHEALRMGSEVFHNLQKVLKGKGFSTGVGDEGGFAPNLDSNEAG
                     LKVIVEAIEKAGYVPGKDVALAMDVAASEFYNSEKKLYVLGDDKKELTSKEMVDMYED
                     LINRYPIVSIEDGLGENDWEGWKLLTDRLGKRIQLVGDDIFVTNTKIIAEGIKQGIAN
                     SVLIKVNQIGTLTETFDAIEMTKRAGYTAVVSHRSGETEDTTIADISVAANTGQIKTG
                     SASRTDRIAKYNQLMRIEEELGEVAQFMGGKSLLNK"
     gene            33792..34013
                     /gene="secG"
                     /locus_tag="PLP02_01935"
     CDS             33792..34013
                     /gene="secG"
                     /locus_tag="PLP02_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPF42255.1"
                     /translation="MDWLDVILLCVSVLLIAVVMLQESKDDVKNTFSGEKSELFKNQK
                     QRGPELLLARITFGASIAFSILAIWAYVR"
     gene            34064..36148
                     /gene="rnr"
                     /locus_tag="PLP02_01940"
     CDS             34064..36148
                     /gene="rnr"
                     /locus_tag="PLP02_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPF42256.1"
                     /translation="MKEELKKIMSDKRYHNTDLFGLARSLQTNDRKGLLSALKEMLAE
                     GDVIEDKKGNYGLSERMGYVKGEIFIKAAGYGFLHVEGREDDLFVPASKTMTALDHDI
                     CLAKIVRSPYDNRTEAQIEKIISRASEIVVGEYYQGAVFPKEEQQGVIYRVTERPKGL
                     VDHAFVVAKIVRFSRSHVLDCAITEVFGYRDDPGIDILEVVKRLDIPYEFPATVMKQA
                     KTLPDEVSDIDIKNRTDLTSEVIFTIDGDDTKDIDDAVSVEILSNGSYKLGVHIADVS
                     HYVTENSSLDKEAYNRSTSVYLADRVVPMLPHSLSNGICSLNPGVRRLAMSCVMDIDK
                     NANVRSYRIFPSVIKSFQQLTYTECNKVLNNEPSEKITDQRIISTLKIMKDLKELLEK
                     AREEAGSIDLETIEPKIILADDGSVKEIKIREHGVTESIIEEFMLMANKTVATDIAKK
                     KLPFIYRIHEDPNEDRLRVLFEIVKGLGHITTIPDPIGKPEIHDLLKSVEDTKYEKVV
                     NMLLLRSMAKARYSDDNKGHYGLGFDYYTHFTSPIRRYPDLLVHRMLRSYLFEKKAQE
                     TKAHYAELLPEAGTWTSKCERRAMTAERETDDMKKAEYMESHIGETFEGVISGVVRFG
                     IFVELANTVEGMVHISSFPEAMEYEEEKMSLLGIASRKVYNIGMKVNVKVASANRRTG
                     KVDFRLALEESS"
     gene            36145..36594
                     /gene="smpB"
                     /locus_tag="PLP02_01945"
     CDS             36145..36594
                     /gene="smpB"
                     /locus_tag="PLP02_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPF42257.1"
                     /translation="MKVIASNKKAAHDYFIIEKYECGIVLTGTEIKSVRLGKVAIKDA
                     YAKIKNYELFVINMHIAKYDKGNIFNHEEKRDRKLLMQKHDIIRLEQKLKTQGLTLVP
                     TRVYLKENLCKIELALCKGKKLYDKRETAKEEDSARRMEKTVRDYQA"
     gene            36607..36952
                     /gene="ssrA"
                     /locus_tag="PLP02_01950"
     tmRNA           36607..36952
                     /gene="ssrA"
                     /locus_tag="PLP02_01950"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            37405..38391
                     /locus_tag="PLP02_01955"
     CDS             37405..38391
                     /locus_tag="PLP02_01955"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF42258.1"
                     /translation="MLLSEETIHKLIEYGKETEFLEYKAIPYYEDNQAEFIKDIDAMA
                     NSRHAGLKCIVIGVNDDGHNNFSPIGIPCEYKNLKDFAHYEDLIQNSITPSVSIEFKK
                     FYIQEKLFVVITIFSDYINGPYSISAPLFLTRYNGKGEKIKDSLQGQMYIRIGTVTRK
                     LNPLEVKEKVNNQGSFDIYLLDSMLFVENDGIGMLPIKMINGFKINKAFTSVNLSILR
                     DSKTIYTRSTHWFETYNTAKPSDDFFVPDYYFEINAFTEKIGTFWFIFGSTDALVCHL
                     DEYGNNTEKLDFKLSFFSTPTGLPMEFVFHDCSIFAMEKVLWKIKLREEKNR"
     gene            38398..39618
                     /locus_tag="PLP02_01960"
     CDS             38398..39618
                     /locus_tag="PLP02_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42259.1"
                     /translation="MKNGKTDSKHEIKNKESIDRILKSYEKYFEYYEEFKEDLLTFFE
                     KKNQIKNWHKICSIQDWINVTVNRIKNLRFDEFQTLSTDDKTFFIIELITCINVLVDS
                     TEGLKYIIGLTTDEYLVFINALDSFQKTKKMNHEHDSYLGITNRNDRDYFKHIRAAFG
                     QHPSNLEINGKRVFASWTLSYDMHEEDLYIFVYVNDPNAPWGYRENIIISDLLLFYKN
                     FFDVALKLLEILRKKVQGFFDELVVDSLYNKDDNLYTKILKTKKGIIKRYHGDEKSYY
                     YICLVEIDEGLHKISKNIQMLFKSEYNEYIRILYDAFCEIHERVKNCSKENLVIFSEV
                     DVSWVDISKYDYIPKKAEAYMDGDKRHYDNYLYSCKEIADVLEFDAIEIKTFADYRFL
                     KYVLKKRNLFVFKE"
     gene            40108..41205
                     /locus_tag="PLP02_01965"
     CDS             40108..41205
                     /locus_tag="PLP02_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF42260.1"
                     /translation="MIKKFSVKNFKNFKDEITIDFSNKRDYEFKQDLIKNGLVNKMLI
                     YGKNNTGKSNLGAAIMDITCHLTDNQHENILYNYYMNGDSIESNVDFNYLFLLENQEI
                     EYKYSKSPNRQLVFEEFIVNGKSIYKYNYKNNQFENLISEAQTIDMSKRNREMSALKF
                     IYVNTMYWPENSPVKLMIEFVKNMLWFRSLKQNEFMGLMPNGENLSDFIINNRLTSHF
                     QNFLSECGQEYHLQEMSEMGKKVLGVKYKNFTARFEAIASTGTLSLWLFFYWMNRTTD
                     ISFIYLDEFDAFYHFELSQYILRYINSKREIQSILTTHNTFLIDNDLLRPDCYFALKD
                     GKIRSFADSTKKVIRQGHNLEKMMISGEFDE"
     gene            41198..41569
                     /locus_tag="PLP02_01970"
     CDS             41198..41569
                     /locus_tag="PLP02_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42261.1"
                     /translation="MSKILIIVEGADEEETLRKGTHNLLSLIDPQKDFIVIPFRNPIY
                     ELYEQYKLGSYDDIVKYLRFEKGLILDSNTLSKTAFSSIYMIFDYEPNYQKYSDEVIK
                     DILNTFNNETENGKVYINYPM"
BASE COUNT        12116 a         9025 c         9857 g        10623 t
ORIGIN      
        1 gtcgtttgtc accgtgaatt ttttcagact ccaaagggga gccaccagtt cttttctcgg
       61 acaatcgccc gtgagtctgt atatgatgat gtccggtcgc aacaattcga tctgatcggc
      121 tgttgtttcg atataatccg agcgcgacat catggttatc tcgccttttt catacaggtc
      181 ggcgagcctg gtgtttttca tcacgtaaag catgtggatc ttgattccct ggatatcctg
      241 tttgttcaga aaacggattg tgtcaagcat catgtcaggc gtttcgcccg gtaggccgtt
      301 gatgatatgg catatgacat ggatgtcctt ttttcttagc tttgcgacgg catcgacgaa
      361 cgattccaag gaatgctgcc ggttgatgaa actggcggta ctcgcatgga tcgactgcag
      421 accgagttcc accgtaagga aggtccgttg ccttagttca ccaagatatt cgacaacatc
      481 gtccggtaat acgtccggtc ttgtcgcaat tgacagtccg acgacttcgt ctgacaggtt
      541 tatggcttct tcgtataatt ttttaagtct cgaaagcggg gcgtatgtgt tggtattcgc
      601 ttgaaagtaa ggtatcgtca ggcaaccggg ccatttctta tgcaagactt ccaggctccg
      661 tgcgtattgg accccgagac tttcgccggg atctccggcg aaatcaccgg atcctttttc
      721 cgaacaaaag atgcatccgt cccacgatag cgtaccgtcc cggttgggac aggtgaagcc
      781 gccgttaagg gggattttgg cgattttatg gccgaatctg gcccgataga aatcgttggc
      841 ggtaaagtaa ggtttttcct ttttaacaaa atccatgtta tcacccgtga caactaatta
      901 tatcacaaac gatgtttttt ggttgttcca gcaactaatt atgatataat aaaaacaatt
      961 gggtgataaa ctatgttcaa catcgttact gacagtatct tacatcccga caggctggta
     1021 aactaccgta accgttccgg ttggttcgtt ttcctttatc tcctgattct ggccatattc
     1081 gtttcggctg gaagtatcgt ttattcgata aaaaatcggg gagaaatcaa tttgaccagc
     1141 gaagcgacag gatgcacttt ggtgaacgga ttcttggttt gcgaaggttt gtcgcacgat
     1201 aacagcgtcg ctttcaatgc ttatgattat tcggtttatt tctttgatga ggacgggtcg
     1261 gttgacttgc ttgacgttga tgacgcggat tcgctcattg ttttccaagg acagtcgatg
     1321 tcgatataca tcggcacctc catgatcatg cagacaccgg cttttgccgg catgagcggc
     1381 gacgatgctt tcgactattt catgcggaca ttcgtcaatt cgttttccgt gatgatcgtg
     1441 gttgcggcaa tcatcaccaa cactgctctg cttgcggtga tttcgcttat cgctatgatc
     1501 gttttctccc gactcatcaa atatatatct ttcggcaaaa tatacaaact agtcgtgttc
     1561 gcttcgacac ccgtggcgct catcctcgtt ttcaacaatc ttttgtcatt gccaacaatc
     1621 ttattcatca ttctgatggc tttggcatac cgctcaatct ttctgttgca aagggaattg
     1681 tatatttata caatgatcca caacagccag caacaaggcg gttcggggca gaatatggga
     1741 atgcctgacg ataagatgga aaatgatgat caagacttag acgatgatgg aaatgataat
     1801 gacgatccgg aagaatgatt cttttgtcct gtcttgcaaa acgttgtttt tcgtgatatg
     1861 atattgtaaa atgccaggat agggaagcag tagtgcaccc gatcacttca agagacccgg
     1921 cgaatcggtg cgagccgggg tgatcaacgc atgaactcgt ccctgtagca gttgcggcca
     1981 aaaccgcaga cgttttccgc gttaaggaag agagtgccgg aattatccgg aacaaaggtg
     2041 gtaccgcgat cttgatcgtc ctttatcggg cggtcttttt attttggagg agatagtatg
     2101 agtgagttta ttcccagttt caatcacacc gaaatcgaaa agaaatggca ggacagatgg
     2161 tatgcatccg atcttttcca tgccgtggat tttgataaac ggcccaaata ctacgccttg
     2221 gtcgagtttc cgtatccttc gggaatcgga atgcatgtcg gccatttccg cgcctatatg
     2281 tcgcttgaga caatcgtgcg taaacgccgg atggaaggat ataacgtact gtttccgatc
     2341 gggttcgatg cttttggcct tccgactgaa aactatgcga tccagaacaa catccatccg
     2401 cggatcgtga cggaccaaaa catcaagact ttcacgaaac aactccgcga agccgggttt
     2461 tcttttgatc tagatcgtat agtcgataca actgatccgg aatactataa attcacccag
     2521 tggatatttt taaagatgta tgaacacggg cttgtttatc gcagcaagac ttacgtcaat
     2581 ttctgtcccg actgcaaagt ggtgctttcg aacgaggaat cgcagggcgg aacctgcgac
     2641 agatgcggaa ccgaggtcgt ccagaaagag aaggaagttt ggtttttgaa gataaccgct
     2701 tatgcggaga aactgttgga aggacttaaa attctggaat cgccagaaag gatcaagacc
     2761 gaacaggaaa agtggatcgg tcggtccgac ggagccttca tcagtttccc gattgccgat
     2821 tctgccgatt cgctcgaggt cttcacgacc cgtccggata cgatatacgg agtgacattc
     2881 atggttatcg ccccagagca tccggcgata aaaaaatatg aagaccggat caagaacatg
     2941 gatgcgatca aggattatgc cgagaattcg aagagaaaaa gcgaattcga aagagtacag
     3001 ctagtcaaag ataaaaccgg gttgaaaatt gttggtttgg aagccgtcaa tcccatcacc
     3061 aaagagaaaa ttccggtgtt catcgccgat tatgtgatga tgacttacgg aaccggagcc
     3121 atcatggccg ttcccgggca tgatcaacgg gactacgaat tcgcgaccaa atacagccaa
     3181 aagattgtag aagtcatcaa aggcggggat ctgtcaaaag aagcctacac cgacatcgaa
     3241 gatggcgttc tggtgaatag cggcatcctt gacggtctta aagtgaaaga agcgattgcg
     3301 aagatgatcg cttacctagg agaattggga tgcggcaaga aaggcgtgca gttcaaaatg
     3361 aaggactggg cgttcaaccg ccaacgttat tggggcgaac cgatcccctt ggtttattgc
     3421 gacgattgcg gcatcgttcc ggtgccatac gaagatttgc cggtgacttt gccgatggtc
     3481 gacagtttca taccgacgga tacgggcgaa agcccgcttg cgaacatcaa ggaattcgtg
     3541 gaatgcacct gtcctaagtg cggaaagaca gccagaagag aaaccgatac gatgccccaa
     3601 tgggccggat cttcatggta ctttcttcgt tacattgatc cgcatgacaa aacggttttc
     3661 gctagccgcg acaaactgga ttactggggt caggtcgact ggtacaacgg tggcatggaa
     3721 catgtcacga ggcatctgat ctattcacgg ttctggaatc tgtttcttaa cgacatcgac
     3781 gaagtgccgt ttgccgaacc ttattgcaaa cggacagccc aagggttgat actcggaagc
     3841 gacggggaga agatgtctaa atcgaaaggc aattcggtca atcctcagga tatcatcaat
     3901 gagtacgggg ccgacacctt gagaacatac attcttttca tcggtgatta tgagcttccg
     3961 actccgtgga acgacaacgg catcaaaggc tgtcgccggt tcttggacaa gatctggcgc
     4021 gcgtttccta aggtgaattc tgttcccgga ttttccatag aaagcgaaat actcatgaac
     4081 cagacgatca agggagtctc ggacgattac gaaagtatga aatataatac ggcgatcgcc
     4141 aagatgatga ctttgatgaa tgaactggga cagaaggaag aaatcaccag gggcgaatac
     4201 gaaacattgt tgaaactgct ttatccggtc gctccgcaca taacggagga actcttcgaa
     4261 aaacttggca aaggccactg gctcgacaag gaatcatggc cagcatggaa tgaggagaaa
     4321 ctggcggaag acaagatcga aatcgttgtg agcgtcaacg gcaaagtccg tgacaaactg
     4381 acagtccccg tcggaaccgg ccgcgacaag ctggaaaaac tggctttgga acttccgaaa
     4441 atccgggaaa taacgaaaga caaccaggtg cgcaaagtaa tcgtggtcga aggcaaactc
     4501 gtcaatctgg tgatataaag aaaaaacatc cgtcaagacg tagtcttttg acgggtgttt
     4561 taatgaaaga gtatcttcat gaaaaaaatc gcgaagcgaa agaaaaactc aaagcaatcg
     4621 ccaatggcat gtgcaaccgc tgcttttcgc ataatctggc gaatgattgc catgacaagc
     4681 tttattgtcc cgattgtctt gtgacggaag acgattatat ttttcgctat gagcgcgagc
     4741 tgccttattg cgatcctttc ataagaccgg atctggtact tacaaaaaga caaagcgacg
     4801 cctcccgttt tcttctcgaa ctgctttccg accggaaaag cgggaattta ttcgcggtct
     4861 gtggagccgg gaagacggag atgacttatc ttgtcgcttc cgaagccatc agaaggggaa
     4921 tgtcggtatg ttatgccatt ccgcgccgcg aagtggtgag ggaactcgca aaaagactca
     4981 gacgggattt caaaggcatc atggtcggcg aaatgcatat ggaaaaaaaa gcggacgagg
     5041 gatgccagat cgtcgtttcc actatcaatc aactgataaa ttactaccat gaattcggcc
     5101 ttgtcatatt ggacgaagcg gatgcgttcc cttatagtgg taatgaatat ctggaaagac
     5161 ttgtgaagaa agcagctctt ccggatgctg ttttcatcaa catgtccgcg accatgacga
     5221 aaggcggcat aatcgacaca tacatcgtac cggaaagatt ccatggtttc agactggata
     5281 aaccgcgatt tgtgaaaacc ggaaacattc tggaaacatt gaaaaacggc aaattgccgg
     5341 caagattcga aagcaaactg aatttatggc ttgattccgg caaacgcgtc tttgttttcg
     5401 tgccgacggt cgaacttggt aaaaaggtgg cgaaaatcat gaaaaacggc aaattgccgg
     5461 tcgatttcct ctatgctggc aatccggcga gaagaaggat tctgaatgat ttttacatgc
     5521 agaaatttgc gatactcgtg accacaaccg tcatggaaag gggcgtgact tatgatgatg
     5581 tccaggtggc agtgctggcg gccgaccatc ccatattcaa taaagcctgt cttgttcaga
     5641 tatccggacg cgccggaaga tttaaaagtc atccgtccgg ggaagtcgtg ttttactcgg
     5701 aaaccttgac cgggcaaatg atgtcgtgcc ggcgcgagat cgcccggatg aaccggctaa
     5761 ggaacaattc gtcgtgatat gtgctatatg ccggaaaacg tttgtcgccg aactggaatt
     5821 ttccaccttg ttccaaccgc cgattctttg cggcgattgt ctgacactta gaaaagccga
     5881 accgataatg gaggcgatac cgacagataa cggtttgatc gaatactatt atctatgcga
     5941 acaaaacgat ttggacacat ttatgtattc gcgctccagt cattttattg aaaaagccct
     6001 tgaaatggcc atttgccggt ataatgagat cgggtttgtc atcattctcg aaccggatga
     6061 tttcaccacg ctggaatcgt ggtggccctt tgtcaaagaa accgggacca tgatattttt
     6121 ttcgtttttc cgctatgatt ttcttacgga aaaaaaggat ttctgttttt gacaaaaacc
     6181 cgcatatccg tttacattca tcacaattca atgctataat ctaatcagat acatttcaaa
     6241 aggagtgatg aatgtgaaac ttgatgtcag aggcaaggac ggttttgaaa tcactgattc
     6301 aatccgctcg tatttcgaaa agaaactcaa gaaagtcgaa aaaatgtttt ccacggaatt
     6361 agaagcattc gcagtatgta agatttacaa gggcatgtcg aaagtggaga tcacgattcc
     6421 gatcaaattc gtgacgatca gggccgaggt tctcgacaag gacatgtacg ccgccatcga
     6481 caaggcggtt gacaaactgg aggcccagat ccgcaaaaac aaatacaaac tgaatcggag
     6541 cctgcaagaa aaaacaggac tgaaagaaac attcaaatcg gacgaactgg acatcgactt
     6601 gctggaaaaa gaactcatct cgccgatcag gaaaaaacgc atccagctgg acatgttgtc
     6661 gaccgacgag gcgatcaccc agatggaatt gctgggacac gatttcttca tttatcggaa
     6721 cgaagacaag gaagtatgcg ttttgtatct tcgcgacgat ggcaaatacg gtctgatcga
     6781 gacatattaa gtgaccttta aaggtcactt tttttctata gggagcacaa atgggttgta
     6841 tagggctttg tttttgtggt atcatattcc ttgtaaaacg aagaggtgat tccagtgcca
     6901 aaattattcg acccaaacag aaaaatactc aaaaggctgg aaaaaacagc agagaaagtc
     6961 cttgcactcg aagacgagtt caagcaactt tctgatgatg agttaaaagc gaaaaccgat
     7021 agtttccggg aaagatataa agccggtgaa actttggatg atttgctggt ggaagcattc
     7081 gctaccgtca gagaggcatc gaccagggtg accggactga ctccgttcaa ggttcaggtc
     7141 atgggagcca tcgtgatcca tgaaggcaac atatccgaaa tgaagaccgg cgaaggcaaa
     7201 accttgaccg ccgtcatgcc cgcctatctt aacgccatcc ccggggaagg cgtccacatc
     7261 gttaccgtca atgaatatct ggccgggcgc gaggtatccg gcgagatcgg tgaattgttc
     7321 cgctttttag gactcacggt cgggctgaat gtccgcgata ttaccccgga agagaaacgg
     7381 gctgtttaca attgcgacat tgtttattcg accaacaacg aactcggttt cgattatttg
     7441 cgcgaccata tggtcatcta caaggaaaac atcgtccagc gtccgctcaa tttcgcgatc
     7501 atcgatgaag tcgactcgat tttgatcgat gaagccagga cgccgctcat catttccggc
     7561 ggcgagaaaa gcaccgccga aatgtacaaa caggcaaatt atttcgcgat ccgtctgaaa
     7621 gaagaagagg attttgcgat cgacatcaaa gaaaaaaccg tcaacctgac ggaagaaggt
     7681 atcaaaaaag ccgaggcctt cttcaatctt gaaaacctgt atgacatttc caatgtggtt
     7741 ttgttacacc acatcaacaa tgccttgaaa gccaatttca ccatgtccag ggatgtcgat
     7801 tatgtcgttc acgaaggcaa ggtcatcatc gtcgacccct tcaccggccg tctgatgcac
     7861 ggacgtcagt tttccgaggg cttacaccag gcgttggaag ccaaggaagc ggtcgagatc
     7921 aagaaagaaa cgaccatcat ggcgaccatc actttccaga atttcttccg gatgtacaag
     7981 aaactgtccg gtatgaccgg taccgctaag accgaagagg aagaattcac gaacatctac
     8041 aacatgtatg tcgtggaaat tcccaccaac atgccggtca ttcgcattga tgacaatgat
     8101 ctgattttcg ccaccatgca agccaaatac aaggcgttgg ccgatgaaat cgaaaaacgc
     8161 cacaatttag gacagccgat gctggtcgga accatttcga tcgaaagttc ggaacttctg
     8221 tcgagtcttt tgaagcgtaa acatatcaaa cacgacgtct tgaacgccaa gcagcacgaa
     8281 cgcgaagctg atatcgtcgc ccaggcggga cagatgggtg ccgtgacgat cgcgaccaac
     8341 atggccggtc gtggtaccga tatcaaattg ggaccgggag tcaaagaatt aggcggcctc
     8401 gccgtcatcg gaaccgaaag acatgaatcc agacgtatcg acaatcagct ccgcggccgt
     8461 agcggaagac agggcgaccc cggatattcc cggtttttcc tgtcaggaga cgacgatctc
     8521 ttgcgccggt tcggcggcga ccgtttcaag agcatgattt cgatgatcac gacgcagaag
     8581 ggttcggaag ccgaacttcc gcttgattac aagatgtttt ccaaactggt tatgaaagcc
     8641 cagcaccaga ttgaaggaaa caactttgac cgccggaaaa ctgttttgca atatgacgaa
     8701 gtgatgcgca gacaacgcga gatcatctac aagcagagaa ccgatatctt gttcttagaa
     8761 acgatcgagg atacggtgaa ccagatgatc gaatcggttt tgtcatctct cataaaccat
     8821 tatgcggaag accgggacgc gatgtacgag gcattgaaca aatacttcca gaaagacatg
     8881 atcgaccgtg acatcctgaa aaccgaagag aatgccacat cgtatgtttt cgaccaatac
     8941 aagaaagaga tcgaaaacaa gaagtccctg accggggaca aaatatacaa cgacttcctg
     9001 aaagccgtca ctttgcgtgt cgtcgatact tactggatcc agcatatcga tgctatgagc
     9061 gaactgcgtc aggccgtgtc gctccagagt tacgggcaga acaatccgtt ccgggaatat
     9121 caggacaccg gattccagat gttcgaggaa atggtcttca atatccagtc cgatgtcacc
     9181 cgttttgtcc tgaaggccca ggtgagacaa aatacggagc gcgtgcaggt tgccaaaccg
     9241 acatatgcct catccggcaa ggaagacgaa aacaagaaga aaaaaccggt ggtcaaatcc
     9301 gataccacca aagtcggtag aaacgatccg tgcccctgcg gcagcggaaa gaaatacaaa
     9361 tactgtcacg gacgcaacgc cgattgacca aaagggagtt tttccatgaa ccaactcgaa
     9421 ctcaagacca aaatcaacga actgaccgaa tggttagaag aactgggccg gaccgttgac
     9481 gaaaaagaca gacaggaaaa aatccgggat atcgaagcaa aaatgctcgc tcccgatttt
     9541 tggtcgaacg cggaaaaagc ccaaaaggtg atccgggaac aaaacgacca caaaagcgtt
     9601 atcgcggaat accaccgtct ttgtgatatt ctggaaacct tgaacatgac ctacgaactg
     9661 gtttcggaag gcgaagaaat ggatttgggg gaagccgagg aacttatcag agaatttgaa
     9721 gaaaaagacg acgccctgac aatcaggctg ttgcttgata aaccttacga caaacttaac
     9781 gccattctag aatttcatcc cggcgctggt ggcaccgaaa gccaggactg ggctcagatg
     9841 ttattccgga tgtatacgag atgggcggaa cagaacggat acaaggtgac cgtgcttgat
     9901 tatttggacg gcgaagaagc cggactcaaa tcggctacca tcctggtcgc cgggcctaat
     9961 gcttacggat atttaaaagc ggaatccggc gttcatcgtt tggttcggat atcgcctttc
    10021 gacgcttcgg ccagacgcca tacgtcattt gcctccgtgt cggtggttcc ggaaatcgac
    10081 gattcgattg atgtcgatat tgacgagcag gatatccggg tcgacgtcta ccgctccagc
    10141 ggcaacggcg gtcagggagt caacacgacc gattccgctg tccggatcac ccatctaccg
    10201 accaaactgg tcgtcacatg ccaaaacgaa agaagccaga ttcagaaccg ggaaacggct
    10261 ttgcgggttt tgaagggaaa attgtatcag ttggaactgg agaagaaaca gaaggaacta
    10321 gattccttat cgacaaccaa cgtccagaac gcttttggta gtcaaatccg gtcctatgtt
    10381 ttccaaccgt acacgatggt aaaagatcat cgtacaagtt ttgaaaccgc ggcgatcggc
    10441 aaagtgatgg acggcgatct gaacggtttc atcaatgcct atctgagaca gaccaagagc
    10501 agatagcggg aggttatcat ggccaaacat cgctcgcttc gccgaatcgg cagattcgaa
    10561 caatatgctt ttcttaccct tggcgttttg atcatggctt cggcttatta cttttttgtc
    10621 attccctccg gtatcgtcac tggcggagtc accggtcttt ccatgatctt gcaaagatat
    10681 ctgccgaaca ttccgatttc ggcatttgct ttttgctttg acatgatatt tcttgctctg
    10741 tgctttttct ttctcggaaa aggcgaattt gttcgcagta tctacggatc gattctgttt
    10801 ccgggatttc tcgctttgtt tgaaaacacg gttccgaatc cgactttcgg accgaatgat
    10861 cttttgcttg tagcccttta tgccggcggt ctcatcggca tcgggttcgc gatcgttgtc
    10921 cgctacggcg gcacgaccgg cggcaccgat attccgatca agattctgaa gaaaaattcc
    10981 cgactttcac tcgcaacatc ggtgtatctg atcgacggat ccgtcatctt actgggagct
    11041 atcacgagcc agagcggcat cggccagggt ttcatcgacg ctctgtatgc tctcgtcatc
    11101 gtcattttgt caggcaaagt gtccgattct ttcctgatgg gactcgaagg aaagaaagcc
    11161 gtccatgtgg tgacagcgaa accgaaagaa gtaaaagccg ccgttttcgc gcttttctct
    11221 cgcggtatga ctgaaatgcg ggttcagggc ggttatacgg aaaccgacaa gtctttgctt
    11281 gtcatggtga tccaaaacaa cgaatacctt ttcgtgagaa acatcatcac aagaaccgat
    11341 ccggcggctt ttgtctatgt gacgccagca tcggaaatca atggcgaatg gtctcccggg
    11401 gaagaggtgt atatcggaaa tgcccgcaac cgtcaacagt ctcaagaaaa aaaacaataa
    11461 atacatagta tccatatcca cccccctggg caaggacaag gaatacgttg tatccgagga
    11521 tttggtggtc gaataccgtc ttgtgaaaag caagattctt tcgcctgacg attacgcaat
    11581 ctttctggat gtatgcgaat tcgaccgttt ttttcaaaag gccaaacact ggctcctatt
    11641 ccaaccgcgt tcgttaagcc aaacgaggga atatttggaa aaggcggggg cttccgacag
    11701 tttggtggaa aagacgatcg ccaaacttcg ggagaaacat ttgttagacg acgacgattt
    11761 tgcctgtcgc tatgctttga ttcttctaga agacaaaaga ttcggtcaaa gacgcattgc
    11821 tttcgaactt gccaaaaagg gatttgaccc tgaaagcata aaactggctc tttttgaaat
    11881 caatcacgat accatttatg agaacatgaa ctatctgttc gaaaagaaac tgccgacact
    11941 tggcaaatca tctctgaatg cagcgattct gacgatgaaa caccatctcg ggcaaaaagg
    12001 gtacgaagcc tctgatattc aggatataat ggctaaaaga aaaggcgaat tcaaccaatt
    12061 catcgacgaa aagaaacaaa tcgaaaaaga ctatcgcctc gtgaaggcga aatacttacg
    12121 gaaagatctt ccggaagcga agagaaggga tttgaccata caggctttgc taaggaaagg
    12181 ctactcttac gaactgataa aagaaacggt cgaaaggggc taaactatgg attacaagat
    12241 gacggaactg gatttgtatc tctttaatga aggcaaactg aaggaagctt acaaacattt
    12301 cggatctcat ctggtatatg acgaggacgg taaattcctg ggaaccaggt tcaccgtcta
    12361 tgccccacat gccagaattg tctcgatcgt tggcgatttc aacatgtggg actcgcggac
    12421 gcacgtgatg acaaagaccg accaattggg gatattttcg cttttcttac cggatgtcgg
    12481 cgaatgggtc cgttataaat actgcattgt cacttctttc ggacaaacca ttttcaaggc
    12541 agatccttac gccttctttt ccgacaaccg gccggagaca agttccaagg tctacgatat
    12601 cgaccatttc gactggtcgg acagagatta tctcgaaaac cgcaagaagg gcaatccctt
    12661 catggcgccg atgtcgatct atgaggtcca tctcggttcc tggatgacaa aaccagatgg
    12721 atcattccac aaatacaatg aactcgtcga ccacttgctg ccgtatgtga aaagccacgg
    12781 attcacccat atcgaaataa tgccgatcgt cgaacatcct ttggatgagt cctggggcta
    12841 tcagggcacc ggttattaca gcgccacttc gcgttacggc gttcccaagg atttcatgta
    12901 tttcgtcgac atgtgccaca aagagggcat cggtgtgatc atggactggg ttcccggaca
    12961 catctgcaag gatccgcacg gactctatat gttcgacgga gagcctttat atgaatattc
    13021 cgattacagc attcgcgaga atgaagtatg gggcacggtg aatctcgatc tcggaaaagg
    13081 tgcgaccaag agttttctga tttcaaatgc gtttttctgg atccgttatt ttcatgtcga
    13141 cggattccgg atcgatgctg tgtctaatat cctatattat ctcggaaaca gcgcgatagg
    13201 aaccaactat cccgccatcg agtttttgaa gaatctttcg tatgcgatca aggaactcga
    13261 tcattccgtt ttgctatttg ccgaggattc gaccaccttt ccaaaattga cgacatccgt
    13321 tatcgacgga ggcgtcggtt tcgattacaa atggaacatg ggttggatga atgatacgct
    13381 caaatatttc gaaaaagatc cgatctatcg caaatatcat catgacttga ttacattcgg
    13441 gatggtttat gcctattccg aacgttttgt tttacccttg tcgcacgacg aagtggtgca
    13501 tggcaaaaaa gcgctcgtcg ccaagatgcc tggtgattac tggcagaaat ttgccaacta
    13561 cagattgttg atagggctcc agttcaccca tccgggcaaa aaacttttgt tcatgggggg
    13621 cgaattcgcc cagatgcacg aatggaagga caaggaagaa cttgattggt ttttacttgg
    13681 ctatccgatg catgagaaag cggctcggtt cgtcagggac atgctcctgg tctacaagca
    13741 agaaaaagcc ctttatgaac ttgatcacga tccccgcggg tttcgctgga tcgatcagac
    13801 caaccgcgat cagtcaatct atatcttttt gcgtcaggcc ctggatgaaa acgagttcgt
    13861 catcattttg ttgaacatga caccgaacgc atatgaacat ttcaaagtag gagtaccgaa
    13921 agcaggaatc tacacggaga ttttgaacag cgacaaggaa gtgtacggtg gatctaatgt
    13981 gtataacggt atgccgcttg taacaatagc cgaacctaac catggcatga cggattccat
    14041 cgatttaaag gtagctccgc tcgcaatgtc cgtacttaag tacatcggaa acaaaaattg
    14101 aaagtctgtt tggtgaaata agatgaaaaa aacgatcata gcgatgattt tggtcggagg
    14161 ggccggatcg aggcttcagg aaatcacccg ggatctggcg aaaccggcag tgtctttcgg
    14221 cgccaaatac cggctgatcg atttcaccct ttccaacatc gccaacagcg atatcgactg
    14281 cgtcggtctt gtgacgcagt acgagcctta cgaactgatg aactatatcg gttccggagc
    14341 gtcatgggat ctcgatgtca tcgacggcgg gatccatttt ctcacgcctt atacaaaaaa
    14401 agcttcgatt ctctggcaga agggaaccgc ccatgcgatc ggacggtatc tgtggtttgc
    14461 tcaggaattc gaggctgatt tcgtattggt attgtctggc gaccagattt acaagatgga
    14521 ttaccgcaaa ttgtgggaac atcatctgaa ccacaatgcc gatctgacga ttgccgccac
    14581 aagagtgcca aaagatgaag cttcgcgctt cgggattctt tcggtcgacg aagaaagtcg
    14641 ggttttggca ttttccgaga aaccggagga accggacggc aatctggctt cgatgggttt
    14701 atatctgttc aacactgatg ttttagtgga cctgctcaag gattcttccg aagaaagtct
    14761 cgacttcggc atgaatgtca ttccgaagag tctcaaacaa aagaagattg tcagtgccta
    14821 cgaattcgat ggttactggc gcgatgtcgg aacggtagaa gctctttata aagcaaacat
    14881 ggatatgctt gacgatccgg attttctcgg gctcaatgtt tccagaaacc ttccggttta
    14941 ctcgaaatcc ctcaatcttc cgccgcatgt cgtcatggac catgccaaaa tacgacgctc
    15001 cgtgatcgcc gacggatcgg tcgtcgacgg agaagtcagc cattcctgca tcggttacga
    15061 atgtatggtc aaagctcacg ctgtgatcag ggattccgta cttttgccgg gagcagtgat
    15121 cggcaaaaac gtcagattga aaaatgtggt tgtgaaccgt aacaccgtga tccccgacgg
    15181 ctttgttttc gaaccggagt ctatcacgct tttggacacg acaaatctgg aaagggcggt
    15241 gaagtgagat gcctaacctt ttcttagtat gcgatgcctc caacatcggt gctttcagca
    15301 agctcacccg tcaccggatg cctggggctt tgcctttcgg agccaaatac cggttgatcg
    15361 acttcacgct ttccaattgc aagaattccc atatcaccaa tgtggcgatc ttcccttacg
    15421 ggaactaccg ctctttgtct gatcacatcg gttccggtga ccgttgggat ttgaatcgcc
    15481 gcaaagacgg tatttttatc ctgccgccca agaacctgaa cctgacaatc gaggattcga
    15541 tcagttttca acgcatgtac gagcatatcg aatacttctt aagaagcagt caggaatatg
    15601 tcatcgtcac accttccagt ctggtctgga acattgatta tcaggttctg ctatacagcc
    15661 atctggtgtc gaaagccgac attacggagg ttttgacggc tgatcgcaaa cgaatacaga
    15721 cattcgtatt atccaaaatg cttttgttgg aatacattcg caactacgat acgatccctt
    15781 accggaacct gacggaagtg atcgactacg cgaccaatct caagaagaat ccctatcatt
    15841 tcgaacaggc ttgtttcttc atcaacagtc cgtttgaact ctatgaggcc aatatgcagc
    15901 ttctctatcc ggagacgagg gcccaattgt ttcggaagga ccgtcctatt ttttccaagg
    15961 aaaccatgtc ggctccggcc agatatggag acaaggcgaa ggtcagccga agcatcatcg
    16021 catccggagc cttgatcgaa ggcgaagtca agggatcgat catcggccgt aaggcctata
    16081 tcgccaaagg cgctttgatt gaaaacagcg tcatcatgaa ccaatgcgtg attgcatcgg
    16141 gtgcccatgt cgaatacgcg cttctggata aagaaacaaa agtggcggaa aatgccgaaa
    16201 taacgggaaa tctcaatcaa ctgttcgttt cggaaaaaaa gcaggttgtt gtatcgggag
    16261 aagatctcag ggtggttcag atcaccgctg aatgcctacc tttcgttaag acaggaggcc
    16321 tggcggatat cgtcgcttcc ctggcggaaa attacagcct tttaggtgtc gattccagcg
    16381 tgatcatgcc cttgtatcca atcatcaagg aaaaatacaa gatgtttctg gaaatgcggg
    16441 ccgaccagtc gatagagtac ggctccgata aatatcacgt gacgatctat tattacaaca
    16501 atcagaacgt caattactat ttcatcgaat cgttcgactt tttcgaccgt gaaaaaatct
    16561 acggttacga tgatgacggc gatcggtttg ctttcttttc caaggcggcg attgcttttt
    16621 gcgacgcctt cgagaaccaa cccgacgtga tccatatcca tgactggcat atgggtcttg
    16681 ttcctttgat tttgaaagaa gacgagaagt gcaggcatat caagactttg ctgacgatcc
    16741 acaatatcga atatcagggt ttgtataaca gcgatatcat cacaaaactg ggaatcaaga
    16801 attacgacat cataaacaaa aagatcaact tcctggaaac cggtatcaac accgcttcca
    16861 agatcacgac ggtttcggat acttaccggg aagaacttca ttacgagtat tacagtcgca
    16921 atctggtcga cacgatcaat cggagagacc gggattttta cggtattttg aatggaatca
    16981 gcaccgatgt tgatccaatc aacgatctga cgatccaaga aaaatacaat ctgatgaatg
    17041 tcttttcggc taaaaagacc gacaaagccg atcttcagaa acgcatgggt ctaaaacaag
    17101 ggaatgacta cttcatctta ggaatggtga cgaggattgt cgaacagaaa ggtttcgaaa
    17161 tcttattgcc gtcgctcgag gatatcatca ggaacccgat gatccaattt gtcattctgg
    17221 gaactgggga agagcgtttc atcgagaatc ttaaccagtt gaaagaacgc tatccggatc
    17281 aggtttccct gaatatcggt tatgatgcga cggagccggg atatatctat gccggagccg
    17341 acgctttcct gatgccttca agatatgaac cgtgcggtac cggccagatg atcgccatgc
    17401 gctatggcac gatcccgatt gtcaggcaga ccgggggact gaatgacacg gtcgagcatt
    17461 tcgaccagat cacgaaacgc ggaaacggat tcaaatttta caactacgat tcccgcgatc
    17521 tttctttcca gatcaatctg gccttccatg ttttcaagga tgcgccggat gattggaaac
    17581 aattggtgat caatgcgatg aacacgaggt tctggcccca ggacacggcc aaagcttatg
    17641 tcgaacttta ccggacgatg ctgatagaaa atccgtaaac ggttttaagg catccttgtc
    17701 gcttgacaac accaaatcga tgatataatc gaatctgtgg aggtgtttca ccatgaccaa
    17761 aagtattttc cggtccaagg acgttttctg gaaagaattc gcggaccggc tgtcgaaaac
    17821 atacatgaaa gatcctgaca acgccagcat tcgtgagatg ttcaacgtac tcggcaccct
    17881 tgttagggaa gaaatcgccg gcgattggaa aaagaccgac gagatgctca aaaaagcgga
    17941 ttgccgcaaa gtccattatt tctcgatcga atttttatta ggaagactga tgacgaataa
    18001 tctgatgagt cttggcgtcc gggaagtagt ggaagatgcg ttcgccgatg ccgggatttc
    18061 actatccgac atcgaggaat acgaatccga tcccggtctc ggcaacggcg gtctcggtcg
    18121 tctcgccgcc tgctatctcg attcgatggc ttcgcttggc atcagcggat acggacattg
    18181 cattcgttac cgctacgggt tgttccgcca aaagatcaaa aacggctatc aggaagaacg
    18241 tccggacaac tggctggcag acggttatgt ctgggaagtc agaaaagacg aagaagcggt
    18301 ggaagtaccg tttttcggtt atgtcggatt cgaagacggc aagatggttt accatccctc
    18361 ggaacggatc cgggccgtgc cttatgatgt tccgatcatc ggaaaccata acggcaaggt
    18421 caattatctg agactatgga acgctgaacc ggcccgcaag tatccgaaag acatgtcgcc
    18481 tttcgaatat gaaagccagt tggagagaat ttccggtttc ctttatccgg acgatacgac
    18541 ggacgacggg aaacgtttga gactgatgca gcagtatttc ttcagttccg ccggtatcaa
    18601 cagcatttgc cggcaacaca agcaaagata cggcaccttg aaaaacctgg ccgaaatgaa
    18661 tgtcttccat atcaacgaca ctcatccgac tttcatcatt ccggaattga tgcggatatt
    18721 cctggacgaa gaaggcatgg aatgggaaga agcctggaag ataacggaaa aggcggtggc
    18781 ctacactaat cacacaatcc tggccgaagc cttggaaaaa tggccggtgt cgctatttca
    18841 gccccttttg ccaagaatct accagttgat cgaagagatc aaccggcgtt tttgcaacga
    18901 cctgttacag aaatacggtt atggaaagat cgaacagatc aaccaaatgg ccattgtttc
    18961 caacggaatc atccggatgg cgaatctatg cgttgtcgcc tctttttcgg taaacggcgt
    19021 cgctaggttg cataccgaaa ttctgaaaaa atatgttttg aaggatttct ttgacttgta
    19081 tcccgacaaa ttccacaatg tcacgaacgg catcacccac cgcagatggc ttctttacgc
    19141 caatccggaa ttgtcggaac tgctctatga aacgattggg gacggtttcg tgacaaacgc
    19201 cgccaaactc ggagactttt tgaaatatgc ttccgacaaa gaaacaatca aacgtttgtt
    19261 cgagatcaaa agaataaaaa aggaagccct ggccgctcgc atatataaag aacagggtct
    19321 tacgatcgat ccggattcca tttttgacat ccaggtgaag cgtctccacg aatacaagcg
    19381 ccagttgatg aatgccatca atatcatgta tgtttacaaa caactgaaag aagacaagga
    19441 atttcgcaga cggtaccatc cccacacttt cattttcgga gccaaagccg cgggatccta
    19501 ttatttcgct aaaaaggtta tcaaactgat tacgacgatc gccgccttgg tcaatgcgga
    19561 cgaggaaacc aacaaatggc tcaaggtcgt gtttgtggaa aattataacg tatcatacgc
    19621 cgaaatgatc atgccggccg ccgatctgtc cgaacagatc tcgaccgcat ccaaggaagc
    19681 aagcggcacc ggtaatatga aattcatgat gaacggagcc atcacgatcg gcaccgagga
    19741 tggcgccaac gtggaaattc acgaactggt cggcgacgac aatatcgtga ttttcggcct
    19801 ttccgcctct gaggttgaaa aattgtattc cgaaaaatct taccgtccga aagaacttta
    19861 cgataacgac ccggttctta aagaaatcct caatcacctg atcgacggat tcttcccgga
    19921 tgtcgacaag aacgaatttc acgatatttt cgacaaactg gtctatgaag atccgtattt
    19981 cgtattgaag gatttgcccg gataccggga agcccacatg agggcaaacg acttatacaa
    20041 ggataaaaac agatgggcgg aaatgatggt gacgaacatc gccaagagcg gcggcttctc
    20101 ctctgaccgt tcggtagagg aatacgccga gacgatctgg catgtgaata tcgccaaggg
    20161 aaaacacaaa tgaaatcccg gtggatagac ctgacccatt ccatatcgcc tgataccatg
    20221 ccctatcccg gggacccttg cctgatttta gaaaaaaacg aaaaaaaagg gaaagacgat
    20281 ttcgtcttgt cccgggtcac gaccggaatg catcttggca cccatgtcga ttcgttttcg
    20341 catctgataa aaaacggagc cgatatcgtt tcactgccgc cggaccgttt ttacggcaaa
    20401 gcggcgcttg tcagggtgaa acaagttggc ggtctcatta aaacacaaga tgtcatttac
    20461 gaatgggaga aactatccga caagccggag atcttgcttt tggaaaccgg tcaggcaaat
    20521 aaattcggga aaaaggaata ttataccgga tgtcccggat tcgagcccgg gtttgccaag
    20581 gttctggctt cgtttaaggt ccgcctgttc ggtgtcgatc taccgacggt gaaatatgga
    20641 gtcaacggaa acgccgccat gcacagggat ctgttcgaaa acgaaattct gattgtggaa
    20701 agccttgcca atctggacaa gttgccggta tattttgact tttttgccct gcctttgaaa
    20761 atcgaagggg cggaagcgtc gctggtccgg gccatggctc ttgccgattg acttttcctt
    20821 tttacagctt tataatatta atcgataaga ataacttcgg ggcggggtgg aattccccaa
    20881 ccggcggtat agtccgcgag ccttaaaggg cacgaccggg tgcgattccc ggaccgacag
    20941 taaagtctgg atggaagaag tggattcaaa agccctgagt acgggctttt tttatggagg
    21001 aacgaagatg ggaaacaaac gcagcacgaa gtatctgata caggttgcca ttctgggagc
    21061 gattgccggg attctgatga ttccggagct tccgatcgcc tttgtggcgc ccaccttcta
    21121 caaactcgat ctgagcgaag tcgccgtctt aatcggaaca ttttccctag gaccgggcgc
    21181 cggtgcgatg atcgaggtca tcaagattct catcaacttg atggtgaacg gtaccgagac
    21241 tgcctatgtt ggggaacttg gcaacctgat aatcggactg gctttcgttg ttcccgccgg
    21301 cttcgtatac aagcacaaca agacgaaaaa aaatgctata gtctcgcttg cggttggcgg
    21361 tttaatgatg gttcttttcg cttccatcat caattatttc gtcctgattc cggcgtattc
    21421 tttcgcgttc gggatcgata tagattatat cgtgtccctc ggacacgcca tcaacgaaaa
    21481 cgtgaatggc ctgcttggct tcatcctggt ctgtgtcata cctttcaacc tgatcaaagc
    21541 catagtggtt tcgctgattg tgtttttggt atacaaacgg atatcgccat tattgaaagt
    21601 gtccggatga catgaaaaga acacttgcgt ttttcctgtt gatgcttttt ttgccgcttt
    21661 tcactggctg ttccctaaca acaaccagca ccaccgtttc caccgaaccg ggaacaactt
    21721 atgaatatga cgatttttcc gatcttttga taaacagccc cgaagatcag ctcacaaggc
    21781 cggaaacgga ctattacatc tatttttacg gcgagcattg ttatctttgc caaacgataa
    21841 aacaggaggc tctcgcttat atagcctcgc tggaaaccga caaggtttat ctcgtgcagg
    21901 tgtcttccta tgacgatatt aacgaagcca tcgattttcc gggaaccccg tcgatcgctc
    21961 acattgtcgc ccacgaatcg gtcaatgtgg aaaaaggcga aagcggagtg cttggagtgt
    22021 tggaaaacct gacataaaaa aagcggaatt caaattccgc tttttatttg gaaaacgaca
    22081 ttttcgatct ctttgtcgat gggcagatgt acgtatttgt tcaggtcgtt cacgtagtat
    22141 gtacaaaatg atttcagatt gctgttgcca tgaaagacgg tatagacggt ttcccggtat
    22201 ttgaatagat tgccgaagtt gtcgaaataa aggacatatg tcttgatgaa cctgatgttt
    22261 ccccggtcca gccggtattt gatcttgtgc attcccggtc tggatttttc gatccagagt
    22321 ttgacgtttt tcagttcttc ccagaatagg gcggaacatc taccggcttc gatttccgtt
    22381 ttactttcgg aataggttgt cctttggttc ttcctatcta gcataatcat aagggattcg
    22441 tccagatagg tttccctgta gcggtccctg acataaattt ccttttcgcc ttcggctgaa
    22501 gagccgatcc atatgtaatc attcgccctg gcgagaaggt ttggaatcat ggtggcaatc
    22561 attaaaaacg acaccattcc ggtaatgccg agaaagtaaa agaagaattt ctggtatgga
    22621 agatctcccg gatgacggat gataccatcg atctcgaacg ggatcccgat gaggtaaaga
    22681 acgaggaaag ttgcgaagag agagaaaaac acgatgaaaa aaatcagcga tacgtaagcg
    22741 tagctgtgat ttttctttga aatcctggcg gttttgatcc tatcgaaatc ataaccggct
    22801 tctttgtatt ccatcatcca cccctcccag gaaaatatta taacccaaaa atgacgggcc
    22861 cgggtaaaaa gatggcattt ttgttttttt taatgtcaaa acaggtaaat gatgataaaa
    22921 taaaactgtc tttgattccc ttccggaaag tgtgattcta tgaatacgat taaaatcgac
    22981 aaatactaca ggggtatgat cgcccatcgc ggattatccg gcatcgaaac cgaaaacacg
    23041 atcaacgcat acatcgctgc agcaaaccgc agttatttcg gaatcgaatg cgacgtttac
    23101 gcttctaaag atgacaaact gattctaacc catgacgaca ctttgttgag aatgggctta
    23161 ttgaatgtct atataccagg atacaagtat gaagagatca ggcgtttcac cctgatcgac
    23221 cggaaaacgg ggcatttgtc cgataccgtg acgattcctt tgctaaaaga ctatcttgcc
    23281 atttgcatga cttatcagaa gcacgctttc atcgaactca aaagcaatct cacgaacgct
    23341 catgtcgacc aggtcattga tgaagtcaga aaatatcacc ggctggaaga taccagcatc
    23401 atttcttttc atgacaaata tcttgtctat ctgagaaaaa actatccgga catgagtctt
    23461 tacctgcttt cagggacagc agacgaaaag acactagcaa tctgtgaaaa gaaccgtctg
    23521 gggctcgatg ccgaatggca tgccattacc gagacggtgg tcaaaaggtt gcatcttacc
    23581 ggtcttaaag tgacggttta cacggttgat gacaagaatg acgccgagaa actgatcaag
    23641 atgggcgtcg attacatcac caccaacatt ctcgagtgat atgttgaaaa tcgtcacagt
    23701 cggcgcttgc gccggcgatt atgtcggcat atccctaaag cggattttaa aatccgattc
    23761 caatccgggc gtagtcaggc tttcttcggg aggagtggcg agaaacatcg ccgaggatct
    23821 cttccggctc ggggccgatg tcttgcttat ttcctttgcc gggagtgacc ggacgggaga
    23881 attcttggcc aaggaaatga aagatctcgg aatcagtcat aagctcttga taaaagataa
    23941 ggaaagatcg tgctgttatc tggcgatcca tgatcatgac ggaaatctgg aatgcggcat
    24001 caacgatttc tctatcttcg atacaatagg tgcgaaagat ttcgcgcctt gccttcccga
    24061 tttggataaa gcagacatga tcgtgctgga ttgcaatttg tcccacgaag ctttgactta
    24121 tcttatcaag agatacaagg ataaacggat tttcatagat ggcgtcagcc aggcgaaaat
    24181 cgggcgcttg tcgggcctgc ttgaggatat cttccttctt aaggccaata tgggtgaact
    24241 ggccgctttg tccggcatcg acaccaaaga tgactttgcg ccggcaatcg acatattgtt
    24301 tcaaaaagga ttacaaaata ttctcctgac aagcggttcc ggaccgatcc gccttgtggg
    24361 aaaagacgga atcatttcga taaatcctca aagcccggag aaaatcgttt ccacgaccgg
    24421 agccggcgac gccctttatg ccggtctcat cagaggattg tccctaggac tttcaaaaga
    24481 aaaatcattg gagctgggaa atagggccgc ttgcatagtg atggcttgtg ccgcggcttc
    24541 tagtttcttg ctctctaccg atatcatcga catgtgaagg aggtaaactt atgaaaaatc
    24601 cgacaattga agccattcta ggaagaagaa gcgtaaggag ttaccttgac aaaccaattc
    24661 ccgaagtcga tctgaagaaa atcatcgagt gcgggatcta tgctccttcc gcaagaaacc
    24721 ggcaaagctg gcatttcacg gtcatcaccg acaaggacac gatcgaccgg atgagcgaac
    24781 ttgccttaga aggcatggaa aaactgggaa tcgtcaagga cgaaggatat catgtcttct
    24841 accacgcccc cgtcgtcgtc gtgatcagtt ccgaactcga aggctatagc gaactgaact
    24901 gcggctgtgc catggagaac atggcgatcg cagcgaaagc gctcggaatc gattcctgca
    24961 tcatcggcca aacgagatac atgttcaatc agacgaacac cgccgatatc aatctattac
    25021 tcaagatacc ggaaggttac cagcacgatt gctcgatctg tttcggatac ctttccggag
    25081 attatccgga accgaaaccg cggaaagaag gagtagtcga ttacatccgc tagatcaagg
    25141 aaaatacttt tgcccaagac aaaagtattt tttctgtcag ataccgcaaa ggaggcacca
    25201 accatgtgga aagagatata ccaagattgg ctcgacgaaa aatcacttga tcctgcgttg
    25261 aaacaggatc ttgaaggaaa aagcgacaaa gaactggaag atatgttcta taccgatctg
    25321 gagttcggca ccggcggcat gcggggagta ttgggagcgg gaacgaacag aatgaatatc
    25381 tatacgatca gacgcgccaa caccggactg gctaggtatt tagaggaaag atacggacgc
    25441 aagaatcttt cccgcggcgt ggcgatcgcc catgacaacc ggcaaatgtc gaaggagttc
    25501 gcgttagaat cggcgaaggt tttaggctat cacggcatca aatgctattt gttcgaagat
    25561 ttgcgtccga cgccggagtt atcctacgcc acgagatatc tgaacgcgat cgccggtatc
    25621 gtcgtcaccg ccagccacaa tccgccgcaa tataacggct ataagatcta tgacgaatat
    25681 ggctgccagt atacgccgga atacgcgaac cgcatcatct cgctcgtgcg cgacgtcaaa
    25741 gatctgttcc atatcggttt tatgacggaa gaagagctga agaaaagcgg gcttttagaa
    25801 tatatcggca aaaagattga cgacagttat ctggaaatgg tcaaaaccgt ccagattcac
    25861 aaggaattgg aaaaacccct gaaaatcgtt tttacacctt tgcatggaac gagcgctgaa
    25921 ctcgggacaa ggcttttaag cgaatgcgga tacgatattt atcccgtcag ggaacagatg
    25981 gtgcacgatc cgatgtttgg caccgtcaaa tctccgaatc cggaaaatcc cgccgctttc
    26041 gacatggcaa aaaaactcgg aatgatagtc ggagcagatc tattgattgc gaccgacccg
    26101 gatgctgata gactaggtat cgccgtgaaa aaggataacg actacatact tatgaccggc
    26161 aatcagaccg gagccgtgct cttgaattat tttctttcgg aaaaacaaaa attgggactt
    26221 ttaccgaaaa aaggagtggt ttacaatacg atcgtgacct ccgatcttgg cgcgaaggtc
    26281 gccagatttt tcggtatgga agtcttttcg accctgaccg gttttaaatt catcggcgaa
    26341 caagcgagat tcctggaatc gaccgataaa aaatttgtgt tcggttacga ggaatcctac
    26401 ggatacgtgg caaacgacaa ggtccgggac aaagattcgc ttcaggccat gttgcttgca
    26461 tcggaagccg cgacgtatta caatgaattg gagaagaaaa cgctatacga caaactggaa
    26521 gatcttttta agatttacgg ttatcactat gaagcgatga tgaatatcga agaaactggc
    26581 atcgaaggca aacagaaaat cgccagaatc atgggtttca tgcaaaaata ccgtgaaacg
    26641 gcatttgccg ggttcagggt tgacgcgatc gaagattata aggcggggat cggctacaag
    26701 cagaatgaga attatgcttt gacgctcccg gaagcggatg tcgtcaagta tatcttctcg
    26761 gatggttcct ggtttgtttt cagaccttcg ggaacggaac cgaaactgaa aatatatatc
    26821 gcctccgtcg ggaaaaccgc tatagaggcc aaagaaaaga ttgaaaaact tgaaagtgcg
    26881 atccgggaat tgttagaagg catcagatag gaaggaagtc tttcagatgt ggaaatacat
    26941 aattgccatc ggcattgtcg ttttgttggt ggcgatctac ataatttcat atgtgctgaa
    27001 ccaaaagacg gaaatacccg aggagtgcag gaatctcgag gacttttccg cctgtagcac
    27061 atgcacgaac cagagttgca cgatcttaaa gaaagggata ccggaactca atgatcggaa
    27121 atgactggga cgggatcttg aaacccgaat tccagaagga ttatttccgg caactttcgg
    27181 aagacatcag ggaggaatac cggcgttaca cctgctatcc gccgatccat gatgtattca
    27241 atgctttgcg gttgacttcg tatgaaaata caaaagtcct gcttttaggc caggatccct
    27301 atcataatcc agatcaggcc atgggactcg ctttctcggt gaaagaaggc gtcgagttgc
    27361 cgccgtcttt ggaaaacatc ttcaaggaat tgcatgacga tatcggatgc cctgttccga
    27421 catccggaga tttgaccaaa tgggggaagc agggagtatt gttgcttaac gcgattcttt
    27481 ccgtgaggcg ctaccaggcg ttaagccatg acaagtacgg ctggacgatt ttcaccgacc
    27541 atatcatttc gctcttgaac gagaaaaaaa caccggttgt tttcgttttg tggggaagtt
    27601 atgccaaaag caagaagccg cttatcacga atccgatcca tttcgtgatc gaaaacgtgc
    27661 atccgtctcc gctttcggcc tttcggggct tttttggatc caaaccattt tcacgcatca
    27721 acgaatttct ggaatcgacc aatcaggatc ccgtcgattt cagtctatga ccagaggaag
    27781 atatgcataa caaaaacatt cgcaatcttg tttttacggg cctgatggtc gctatcggtg
    27841 tcgttctggc tcagtttctg tcgatttaca ttccgaatgc atccgaaccg atcatcaagt
    27901 tcgggatcgg ttatctgccg ctcatcatgg cttctatcat cgccggcccg gtttacggcc
    27961 tgatggctgg tatcgcccag gatttgcttg gatttttcat cttcggagga ccgtccgggc
    28021 aaacattctt tcccggtttc actttgaacg cggccttgta cgggttttta ccggggatca
    28081 tttaccggat ccggaaaaac agcgggacaa aactgttttt ctggctgaat ttggttttgg
    28141 ctgttttgtt gtttctggga tcgttctggt attttttaca tgtcgatcag gtgatttcca
    28201 gtactttgac cgacaatcag aaactgcttt tggccggaat cggtttgatc ggtaccgtgt
    28261 ttttggtggt gtttgatatt ttggtttaca aaagcaagaa atacggcgat gaaggaacga
    28321 agatcctttt catcatcagc ctgatgtatg tcctgacttc actcattttg acgccgatct
    28381 ggctctatat catgaatccc ggtatttcct tctttgtgcg aattcccctg agaatcgtga
    28441 aaatgccgat cgaggtgtcg ttctatgtca tattggtttc ggtccttttg gaggcgcttg
    28501 gcattaccaa aagaaaactg gggccgggat caaaataaaa cacgactgcg aagtcgtgtt
    28561 ttttatttgg tgacaatcag aattcgatga tcttgacttc cccgccgccc agatgaagat
    28621 tgatccttcc gtcgtcggta aagtcttcat atggtctacc catttcgtag gtcgagtaaa
    28681 ccaggtttcc ggataagttt tcgtttccgg attttttccg taattccgcc aaggaagcgt
    28741 tgcaccagat ttcctggcgg gggttgctgt tggcggcgat taagatgcat ttgccggttt
    28801 ttttgtcgaa ataggcgacg ccgatagccg gcgtgtcaaa ttcgttaaag taaatcggca
    28861 atgaagcgtt tgggtccatg atctggctaa gccagcgttt tcttatctcc ttgattcctt
    28921 gcatgtggtc ggcgatctcc cagcggaatt cactggtgta atggaaagca tatttgtcga
    28981 aaagcgcgag tttaccgtag aaaaggtcgt cttttggcag attgaatttg tctttgtccg
    29041 tgcaatcgac gccggtgttc atcggttgga tctcgtatat ttcctgacca gaattgatga
    29101 acggaaccag attcggaagc aacatgttca atatcgtcag catccttgcg agttccctgc
    29161 ctccatcacg cccggcaatt cttgccgtgt catgggtttc ggcgcaggcg aacatcggca
    29221 aggcgacgtc tttggctcca tagacaaact tgtgcagatt cttttcccag atcctgggtt
    29281 ccatccagaa accgttgccg atgatgatgt tatagtcaga agccttggct ttggctgcgt
    29341 tttccggatt cagttcctcg gcgataaaag cgaagtcggg atcgtattcc cgagccttgc
    29401 tgatgatcat cttgagcagt tccttgggaa gcgcatgtcc catatcgatc ctggctccat
    29461 cgattccaaa ctgttcctgg taatagggaa tgatgcccga cagagtctcc caaagtggca
    29521 tgttgggttt cttgcccggg aagagattcg attttatagt atcaaaaaga atatatggag
    29581 gaatgttctt gtttttcaga tatttgacgt tgtcggacgg atgatccaga aacatccgga
    29641 agaaggtgac atcggtccag gggggctgga cgtcgttgat atggtcggag aacgctgggg
    29701 cgacggaaag accgtaagcc ttttcgatgg cttcgacgat gtcctcggca tccttgattt
    29761 cgttccacaa ttccttgttt tcggtatatg ggtcgttacg aaacatcgaa atgtgtcttt
    29821 ggacatcttc cgaggcatag acgtccggca tgaacttggc ttccggtgga atcgtatttg
    29881 ccagtccggt tacgtaagga acccggtatt tgtcgtagtc tttcgtttcg atccagtaga
    29941 accagtcggg atgaagtctg atgaggtcgc tttcgacgga attggtgcgg gggatgatat
    30001 cgataacgac acgcatgccc aaaatatggc aggcttcgac gaaggcctga aactcctcgc
    30061 ctaccgtcat ctcgccgccg gtcaggttgt ctttcagatc cgggtcgatg tcatagaacg
    30121 atgcgacgcc gtacggggaa ccgagttcgc ctttcttttc tttgcgggaa taacgggata
    30181 tcggcaagag atacatcgtg tcgactccga gtttttggag taatggcaac aaagccagca
    30241 tcttgacgaa agtgccggtt tctttcattt cgtccagatt agacacatcg agcgaatagg
    30301 aacggtcgtg gtcccaagcg gatgacgccc ggatcatggt cgaatagaga acgctttttt
    30361 taacccattc gccaccgagt gttccttttt cgggaatggt gttgcttgat ttggccaagg
    30421 attccaaata gttttggttt gtcttggctt taggaaggat gtatgatgaa atcacttcgc
    30481 tgtaaaagcg gtacggattg accagcagtt catgcgacaa tagcctatgg gtttcctcgc
    30541 cggaatagtt ccaagcgttc caaagatcgg gaaccgtgta attgatgtta ccggatggaa
    30601 ttttgctaac gaggatattt tttagcttca tcaaccggga tccagacata atatcacctc
    30661 acatttaata ttatatcaaa gagatatcaa tttatggaca ctgcttttaa aagatgtaaa
    30721 ggtttacaaa ggtatcatta cactttcaaa agcggggctt ttatagtata ataacagcgg
    30781 ttagaggtga acttatgaaa aaacctgtca tactaatgat cctggacggt atgggcatcg
    30841 gcgaacaaaa tgccggcaat gccatctacc tggccaagaa accgcatctt gatctcttgg
    30901 aacaggaatt tcccactaca cagatagagg cttccgggga agccgtcggc cttccggaag
    30961 gacaaatggg caacagcgaa gtcggacatc tcaatctggg agccggccgg gtgctttacc
    31021 agtccttcac gaggctgaac gtcgccatca gggacggatc tttcaaagaa aatcctgctt
    31081 acaacaaggc tttcgatcat gtgaaaaagc atcattccaa attgcatatt ctcggtctgt
    31141 tgtcggacgg aggcgtgcat tcgcatatca accatatcaa atacatgttc cagattgcca
    31201 aggaaaaagg cgtcgaacgt gcttattttc atgccttctt ggacggacgc gataccccac
    31261 cgaaatcggc acttacctat gtgagcgacc tcgaaagtaa aatggctgaa ctgaatttcg
    31321 gctccatcgc cacggtcgga ggacgctatt acggtatgga cagggataaa aactgggacc
    31381 ggatccagct cgcttacgac gccatgtcgt ttgacaaagg tccacacttt ccgtcggcca
    31441 ctgctggcat ccaggcgtcc tacgccgaca atgtcacaga cgagtttgtg gttccgttta
    31501 tctgcgacga taccgggatg atccagtctg acgatgccat cattttcatc aacttccgcc
    31561 ccgatcgcgc gatcgagatt tcgacggctt actctgatcc ggaagcctgt ccgaagctaa
    31621 acaccgaaaa tcatccggaa aacatcctat atgtctcgac gatgaaatac tccgatctcg
    31681 tcaaaggcga actcgctttc ccgctcatca gtatgaaaga tactttgggt gactatatct
    31741 ctgatctcgg aatgaaacag ctaaggattg cggaaaccga aaaatatgcg catgtcacct
    31801 tctttttcga cggcggcgtc gataaacaga tcgaaggagc caaacgggtt ctgatcccgt
    31861 ctccgaaagt ggcgacttac gacttgaaac cggaaatgag cgcttacgag atcaccgata
    31921 gcgtgatcgc ggaactcgat accggcgaat acgatatcgt cgttctgaat ttcgcgaacg
    31981 gcgacatggt cggtcatacc ggcgtgataa gcgctgccat caaagcagtc gagaccgtcg
    32041 acgagtgcgt cggcagggtt gtagacaaga ttcttgagat ggacggcatc gccttcatca
    32101 ccgccgatca cggcaactgc gagaagatgc tgacggaaga cggaaaaccg ttcaccgccc
    32161 atacgaccaa caaagtccgc ctgattgtga cgaaaaaagg catcaccctg aaagacggcg
    32221 gcgtcttgtc ggacattgcc ccgtcgatgc tggatttgat ggatttgaaa caaccggaag
    32281 ccatgaccgg acattcccta ataataaggg acaaggaata ggcaacaaac tgtactgaca
    32341 gataaacaaa taaaatgtca aagtcaaagg agaaaaccat gccaaacatc acaagcatca
    32401 atgcaagaga agtattggat tcacgcggca acccgacagt cgaagtcgaa gtttacaccg
    32461 aatccggagc tttcggaagg gcgatcgtcc catcgggagc ctccaccggt gaaagggaag
    32521 ccatcgagct tcgcgacgga gacaagagcc gctatcttgg caaaggcgtc ttgaaagccg
    32581 tcagaaacgt caacgaaatc atcgctcccg aactgatcgg ttacgacgtc accatgcaga
    32641 catacatcga caaagtaatg atcgacctcg acggaacgcc gaacaaatcg aaactcggag
    32701 cgaacgccat tttaggtgtc tcgatggctt gcgccaaagc agccgccgac tgggccgggc
    32761 ttccacttta tctctatctc ggcggattca acgccaaggt gctccccacc ccgatgatga
    32821 acatcatcaa cggcggatcc catgcggaca acaacatcga tttccaggaa ttcatgatca
    32881 tgccgattgg cgccaaggat ttccacgaag ctttaagaat gggttccgaa gtcttccata
    32941 acctgcagaa ggttttgaaa ggcaaaggat tctcgaccgg tgtcggcgac gaaggcggat
    33001 tcgctccgaa tttggattcg aacgaagccg gactcaaagt gatcgtcgaa gcgatcgaaa
    33061 aagccggata cgttcccggc aaggacgtgg ctttggccat ggacgtcgcc gccagcgaat
    33121 tctacaactc cgaaaagaaa ctttatgttt tgggagacga caagaaggaa ctgacaagca
    33181 aagagatggt cgatatgtat gaagatctga tcaaccgtta tccgatcgtc tcgatcgaag
    33241 acggacttgg cgagaacgat tgggaaggct ggaaattatt gaccgataga ctcggaaaaa
    33301 gaatccaatt ggtaggcgac gacatcttcg tcaccaatac caagatcatt gccgaaggca
    33361 tcaaacaggg aattgccaac tccgtcctga tcaaggtcaa ccagatcggc acacttacag
    33421 agactttcga cgccatcgaa atgacgaaac gcgccggtta taccgccgtt gtcagccacc
    33481 gttccggtga gaccgaagat acgacgattg ccgatatctc cgtcgctgcc aatacgggcc
    33541 agatcaagac cggttccgct tcccgtacag accggatcgc caaatacaac cagttgatga
    33601 gaatcgaaga ggaactcggc gaagtcgcgc agttcatggg cggaaagagc ttgctcaata
    33661 agtaatcacc aggcatctta taacacctaa aaaacatgct tttgggtctt ccaaaagcat
    33721 ttttttatgg tataatagtt tagtgcaatg cataggtcga tgtttcagac aggttttcag
    33781 gaggtttttt catggattgg ttagatgtta tattgctatg tgtatcggtg cttctaatag
    33841 ccgtggtaat gcttcaggag tcgaaggatg atgtcaagaa cacgttttcc ggagaaaaat
    33901 cggaactgtt caagaatcag aaacagcggg gtccggaact gcttttggca aggattacct
    33961 tcggagcctc gattgcgttt tcgatcctgg ccatctgggc ttacgtacgc tagaacacat
    34021 tgccatgtgt tctttttttt gcctttgaag ggaggtggtc cgaatgaagg aagaacttaa
    34081 aaaaataatg agcgacaaaa gatatcataa taccgatctc ttcggactgg ctcggagctt
    34141 gcagacaaac gaccggaagg gccttctttc ggcattgaag gaaatgcttg ccgaaggcga
    34201 cgtgatagag gacaagaagg gcaattacgg tctttccgaa agaatgggtt atgtcaaggg
    34261 cgagattttt atcaaagcgg ccggttatgg atttcttcac gtcgaaggcc gggaggacga
    34321 tctcttcgtt cccgccagca agaccatgac ggcgcttgat cacgatatct gccttgctaa
    34381 aattgtaaga agtccatatg acaaccgcac ggaagcccag atagagaaaa tcatttcccg
    34441 ggccagcgaa atcgtggtcg gagaatatta ccaaggagcg gtgttcccga aagaggaaca
    34501 acagggagtc atatacagag taacagaaag gccgaagggc ctggtagacc acgcttttgt
    34561 ggtcgcgaaa atcgtcagat tctcacgaag ccacgtcctt gattgcgcga ttaccgaagt
    34621 cttcggttac cgcgacgatc cgggtatcga tattttagaa gttgttaaaa ggctggatat
    34681 tccttatgaa tttccggcta ctgtcatgaa acaagcgaag acgttacctg acgaagtgag
    34741 cgatatagac ataaaaaaca ggaccgatct cacaagtgaa gtgatcttca cgatcgacgg
    34801 cgacgacacc aaggatatcg acgatgccgt atcggttgaa atattgtcaa atggttcata
    34861 taaactcggc gtccatatcg ccgacgtcag ccactatgtg acggaaaact caagcctcga
    34921 caaggaggcc tacaaccgct cgacgagcgt gtatctggcg gaccgggtcg tgccgatgtt
    34981 gccacactcc ttatcgaacg gcatctgttc tttgaatccg ggagtaagaa gacttgcgat
    35041 gtcttgcgtc atggacatcg acaaaaacgc caatgtgcgt tcctacagga tattcccgtc
    35101 tgttatcaag agctttcagc aactgactta caccgagtgt aacaaggttc tcaacaacga
    35161 gccatcggaa aagatcaccg accagaggat catatcaacc cttaaaataa tgaaggattt
    35221 gaaagaattg ctggaaaaag ccagggaaga agccggttcg atcgatttag aaaccatcga
    35281 accgaaaata atcctggccg acgacggcag tgtcaaggag atcaaaatcc gggaacacgg
    35341 cgtgaccgag tcgatcatcg aagaattcat gttaatggcg aacaagaccg tggcaaccga
    35401 tatcgccaaa aagaaactgc ctttcatcta ccggattcac gaggatccga acgaggaccg
    35461 gcttcgcgtt cttttcgaga tcgtgaaagg tctaggccat atcaccacga ttcccgatcc
    35521 gatcgggaaa ccggaaatcc atgatttgct taaaagcgta gaagatacca aatacgaaaa
    35581 agtcgtcaat atgctgttac tccgttcaat ggccaaagcc agatactctg atgacaacaa
    35641 gggccattac ggcctcggct tcgactacta cacccatttc acatccccga tcagaagata
    35701 tccggatctg ctggtgcata ggatgctaag gagctacctg ttcgaaaaaa aggcccagga
    35761 gacaaaagcc cactatgccg aattgcttcc tgaagccggc acctggactt cgaaatgcga
    35821 gcgccgggcc atgacggccg aacgggaaac cgacgacatg aagaaagccg aatacatgga
    35881 atcgcatatc ggcgaaacat ttgaaggcgt catttccggt gttgtccgtt tcggcatttt
    35941 tgtcgaactg gcaaataccg tcgaaggaat ggtccatatt tcttcctttc cggaagccat
    36001 ggaatacgag gaagagaaga tgtccttgct tggaattgcc agccgtaagg tgtataatat
    36061 aggtatgaag gtgaatgtga aagtcgcctc agcgaaccga agaaccggaa aagtggactt
    36121 taggcttgca ttggaggaat cgtcatgaaa gtgatcgcca gcaacaaaaa agccgcccat
    36181 gactatttca tcatcgaaaa gtacgagtgc ggcatcgttc tgaccggaac ggagatcaaa
    36241 tccgtccgtt tgggaaaagt cgcgatcaaa gatgcctatg ccaaaatcaa gaattacgag
    36301 cttttcgtca ttaacatgca tatcgccaaa tacgacaagg gcaacatctt caatcacgag
    36361 gaaaaaagag ataggaagtt attgatgcaa aaacacgata tcatccgtct cgaacagaaa
    36421 ttaaaaaccc agggacttac cctggtgccc acaagagtct atctcaagga aaatctctgt
    36481 aagatagaac ttgccttatg caaaggcaag aaactctatg acaagagaga aactgcaaaa
    36541 gaagaagact cagcgcgccg gatggaaaag acggtgcgcg actatcaggc gtaagcctga
    36601 ccctatgggg ctgttttgga ttcgacagga cagttcgagt ttgacaagca tgcgatcggc
    36661 agatctcaaa atgcacccag taaatataac tggaaaccaa aattacgctt acgccgccta
    36721 agaagaggta gcgagtccgt ttttaccagc ctgcgggtct aaatgggcta cagctaagca
    36781 ggatagcgaa gaatgtcgct gttctaggca ttttagcgaa aataacagga ctggttgtcg
    36841 gggtattggt tttctttttc cccgccaatg agataaacaa ggaaactaag catgtagaaa
    36901 gtcaaatgat tggctgtttt ggacgggagt tcgattctcc ccagctccac caaataatcc
    36961 cgtaacctga gaaaaacagt atttacatat tgtatatttc tcgggctttt ttattaataa
    37021 aattgtgttt atcatacata ttttggcaat caaaataata attattttat attaaaataa
    37081 tgatactcaa aagtaatata atattgatac atataaaatt tattcgatta tatgccaaaa
    37141 aattaaatat aaagcaaaca ttttacataa tatttatgac attaacaggt gaaatagtgt
    37201 ataagtatat tgggtatttt tgcaaataat ttttttatta acgagttatt gcatatcaag
    37261 aatcttatta gtagtattta atgttatttc aattatcagt gaattattat gtaaaaatta
    37321 aaaaaactgt aagaatgtaa tcgatcaaac catatttttg tgcaataaaa taaacacgaa
    37381 taaaaaagag aggtaattca aaaaatgttg ctttcagaag aaacgataca taaattgatt
    37441 gaatacggaa aagagacaga atttctagaa tataaggcca tcccttacta tgaagataat
    37501 caagcagagt ttattaagga tatagatgct atggcaaatt caagacatgc cggtctaaaa
    37561 tgcatagtga ttggtgttaa tgatgatgga cataataatt tttctcctat cggtatacca
    37621 tgtgaatata aaaatttaaa ggattttgct cattatgaag atttgataca aaacagtatc
    37681 actccatcag tttcgatcga gtttaaaaag ttttatatac aggagaaatt atttgtagta
    37741 atcactattt tttcagatta catcaacggc ccatattcta tatcagcgcc gcttttcctt
    37801 actcggtaca atggaaaagg agaaaagata aaggacagtt tgcaagggca aatgtatatt
    37861 cgtataggaa cagtaacaag aaaacttaat ccgttagaag taaaagaaaa agtaaataat
    37921 caaggctcat ttgatatata tttactagac tctatgctat ttgtcgaaaa tgatggaatt
    37981 ggaatgctac caataaaaat gataaatggg tttaaaataa ataaagcatt tacttctgtt
    38041 aatttatcaa ttttgagaga tagcaaaaca atttatacaa gaagcacaca ttggtttgaa
    38101 acgtacaata cggcaaagcc atcagatgat ttttttgttc cagattatta ttttgaaatt
    38161 aatgcattta ccgaaaaaat aggaacattc tggttcattt ttggctcaac agatgcgtta
    38221 gtgtgtcatt tggacgaata tggtaataat acagaaaagt tggattttaa gttaagtttt
    38281 ttttctacac ctacaggttt gccaatggaa tttgtttttc atgactgttc gattttcgct
    38341 atggaaaaag ttttatggaa aatcaaatta agagaagaga aaaacaggta aaaaaatatg
    38401 aaaaacggaa aaaccgattc aaagcatgag ataaaaaata aggaatcaat tgaccgtatt
    38461 ttgaagtcat atgaaaagta tttcgaatat tatgaagaat ttaaagaaga tttactcaca
    38521 tttttcgaga aaaaaaatca aataaaaaat tggcataaaa tatgctctat tcaagactgg
    38581 attaatgtta cagtaaacag aataaaaaat cttagatttg atgaattcca aacactgagt
    38641 acagacgata aaactttttt tataattgag ttaataacat gcatcaacgt tttggttgat
    38701 agtacagaag ggctaaaata tataattgga ttaactacag atgaatattt agtttttata
    38761 aatgctttag atagttttca aaaaacaaaa aaaatgaatc atgaacatga cagttatttg
    38821 ggaataacaa atagaaatga cagagactat tttaaacata taagagctgc atttggccaa
    38881 cacccttcaa atctcgaaat aaatggtaaa cgagtatttg cttcttggac attgagttat
    38941 gatatgcatg aagaagatct ttacattttt gtatatgtaa acgatcctaa tgccccttgg
    39001 ggatatagag agaatataat aatttcagat ttattgcttt tttataaaaa tttttttgat
    39061 gtggctttaa aattattgga aatattacga aaaaaagttc aaggtttttt tgatgaatta
    39121 gttgtagata gtctttataa taaagatgat aatttatata caaaaatatt gaagacgaaa
    39181 aaaggcataa taaaaagata tcacggagac gagaaaagtt attattatat atgcttagtg
    39241 gaaatagatg aaggattgca taaaattagc aaaaatattc aaatgttatt taaaagtgaa
    39301 tacaacgaat acatacggat tctttacgat gctttttgtg agattcatga gagagtaaaa
    39361 aactgttcaa aagaaaatct tgtaatattt agtgaagtag atgtatcgtg ggtagacatt
    39421 agtaaatatg attatattcc taaaaaagct gaagcttata tggatggaga caaaaggcat
    39481 tatgataatt atttatattc atgcaaagaa atagcagatg tgttagaatt tgatgcaata
    39541 gaaatcaaaa catttgctga ctataggttt ttaaaatacg ttttaaaaaa aagaaatcta
    39601 tttgttttta aagagtaaat tggtaattag cgataagcta ttactttttt gataaattag
    39661 gctttttatt aatgccaata ttattttaac aaaagctaaa tatattcaat atagaaaaac
    39721 aatctccact agtattattc gcgtacacac gtatgtttta tggattccca gctccacaat
    39781 ttcaacccat catccgagaa aacgacattc acgcagaaaa gtcatgcttt cttgggcttt
    39841 tcatagtaaa aagattgtct attatgagaa aaacgaataa ctaataccca tgcaaatgtt
    39901 gttgtaatat agaatattat agattttggt atgctgatga acaaaaaatt aagcaagcac
    39961 gacaattagt ttttttctgt ttaatcacat ccactaatta aaattcagaa aattcaaata
    40021 gagtatcgcg cggaatgaat gtaaaatttt tacattatgc ttgtaatttt atttcgacta
    40081 gtttataatg tggttgaggt gataagcatg attaaaaaat tcagcgtaaa aaacttcaaa
    40141 aacttcaaag atgagattac tatcgatttt tcaaataaaa gggattatga gtttaaacag
    40201 gacttaatca aaaatgggct tgtaaacaag atgcttatat atggaaaaaa caataccggc
    40261 aagagtaatt taggcgcagc aattatggat attacttgcc acttaacaga taatcagcac
    40321 gaaaacattc tatataatta ttatatgaac ggggattcta ttgaatcaaa tgttgatttt
    40381 aattatctct tcttacttga aaatcaagaa attgaatata agtattctaa atctccaaat
    40441 cgtcagttgg tttttgaaga attcattgtg aatggtaagt ctatatacaa atacaattat
    40501 aaaaataatc aatttgaaaa tttaataagc gaagctcaaa caatagatat gagtaagaga
    40561 aatagagaaa tgtccgcact caagtttatc tatgttaata ctatgtattg gccggagaac
    40621 agcccagtaa aacttatgat tgaatttgta aaaaatatgt tgtggttccg atcactgaaa
    40681 caaaatgaat ttatggggct tatgccaaac ggggaaaacc tcagtgattt cataataaat
    40741 aacagattga ctagccattt tcagaatttt ctaagtgaat gtgggcaaga atatcactta
    40801 caagaaatgt ctgaaatggg aaaaaaagta ttaggtgtga aatataaaaa ctttactgct
    40861 agatttgaag caattgcgtc aacaggaacc ctatcattat ggctcttctt ttattggatg
    40921 aatagaacga ctgatatttc attcatttat ttggatgaat ttgatgcatt ctatcatttt
    40981 gagttatcac agtacatctt gagatatatc aacagcaaga gagaaatcca gtctatttta
    41041 acaactcata acacattttt aattgataat gatcttttaa ggccggattg ttattttgct
    41101 ctcaaagatg gaaaaattcg gagttttgcc gatagtacaa aaaaggttat cagacaaggg
    41161 cataatttag aaaaaatgat gatttcagga gagtttgatg agtaagattt taatcatcgt
    41221 tgaaggggct gatgaagagg aaaccttacg taaaggtact cacaatttgt tatctttgat
    41281 tgatccccaa aaggacttta ttgttattcc atttcgcaac cccatatatg agctttacga
    41341 gcaatacaaa ttagggtcat atgacgacat tgtaaaatat ctgagatttg aaaaggggct
    41401 gattttggat tccaacactt tatcaaaaac agctttttct tccatttata tgatttttga
    41461 ctatgaacct aattatcaaa aatactctga tgaagtgatt aaagatatac tgaatacatt
    41521 taataacgaa acagaaaatg ggaaagtcta tattaattac cctatgtgag ttagtttgat
    41581 taaaacggac agttaaataa aaagagtgga aagaacttgt t
//
LOCUS       DAOPJL010000008        39658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1064_length_39658_cov_8.960003, whole genome shotgun sequence.
ACCESSION   DAOPJL010000008 DAOPJL010000000
VERSION     DAOPJL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 39658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39658
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_39658_cov_8.960003"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLP02_01975"
     CDS             <1..602
                     /locus_tag="PLP02_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF42262.1"
                     /translation="SVRRVKDWFLDDAVNGDVDAQYLIAHVYNSYTSQQEHHYWLKKA
                     ADQGHSAAQYKFGIENKDENPSDYIEYIKLASKSNVYAQIELGYNYAHGQYVCKSYLD
                     AYNLYQKAADNMKKIEDIRELRIINNIKIRYNAANDEAEQLALNDDVDAQLYMGCLYQ
                     YGFEIKRNKEKAVYWYELANTNGSVEASEQLIIIDNEMI"
     gene            844..2598
                     /locus_tag="PLP02_01980"
     CDS             844..2598
                     /locus_tag="PLP02_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF42263.1"
                     /translation="MNQNVLNEAIKRFECSDYEKAIYLFKSIADDHVLAMYYLGSMYR
                     MGLGVKEDHKEGFKLFLKAAKLGHAESQFLVGCAYTGSGIYNHSETELVRFDIETILE
                     KSVKDNSVWQDSIPFYHLDGIGVEPNNKEAIKWLSKAAEQNYVNAQIVLGDIYNWGVI
                     VDNDKEKAKQWYEKAASNQNSKAVRELAFFFSRNKNDLANKIKMLIKAHALGDNLAAY
                     FLGKTYERDIKEEGHIEQAVYWYKIGAETQNCYNSQLELGEFALNGIGQKKDIKQAVT
                     WYKKAIETFNLIHHYSHYGQANERLIDLYEKGYKDLITENEYIEYLKGAVESSKDSAR
                     YKLREYYKKGYDVGEENKKFFELLDSAQQGDKNAQIKFGYLYIVDKFIDESTIRIVKD
                     WFLNDAVNGDVDAQYLIANVYNSYTSQQEHHYWLKKAADQGHSAAQYKFGIENKDENP
                     SDYIQYIMLASESNVDAQIELGYNYAHGKYVCKNYLDAYNLYQKAADNMKEIDDIEEL
                     RTINNIKIRYNAANDEAEQLALNGDVDAQLYMGCLYQYGFEIKRNKDKAVYWYEMAKK
                     QGSNEAQIQLNLLYMELE"
     gene            2602..2955
                     /locus_tag="PLP02_01985"
     CDS             2602..2955
                     /locus_tag="PLP02_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42264.1"
                     /translation="MDEKRLKKVLIEIKNGLGLDYALHKGFVSPTCTAKKLDVLYGDN
                     SIGIYVRWFGKDQEQPQIRNMDKLYINHDFGSENSDFRKSEIVNILSKYYDVDWDYSD
                     KKSILIKEKGESKDA"
     gene            3766..4062
                     /locus_tag="PLP02_01990"
     CDS             3766..4062
                     /locus_tag="PLP02_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPF42265.1"
                     /translation="MPFCPKCGAEVKENQDVCLSCGTAISKNATSIVSEEGSTTGWGV
                     LGFFFPLIGLILYLVWKTDKPKAAKSAGKGALIGVIAGFVLGILEAIIVIAAGI"
     gene            4108..4425
                     /locus_tag="PLP02_01995"
     CDS             4108..4425
                     /locus_tag="PLP02_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPF42266.1"
                     /translation="MPYCSNCGAELKDGQQVCLSCGTFVKTNKANPKAIDDGKTDAGF
                     FLIGFFVPLAGLILYLLWREEKPKEGRSAGKGALVSVIVSAALGILWIVIMLLVFPFA
                     FGI"
     gene            4752..6137
                     /locus_tag="PLP02_02000"
     CDS             4752..6137
                     /locus_tag="PLP02_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPF42267.1"
                     /translation="MQKKIIVPPYKIKMVEPIHIIDKKTRMERLEKAGYNPFKLRSED
                     VFIDLLTDSGTGAMSQFQWAALMQGDEAYAGSSSFYRLEKTVKEITGYEYVMPAHQGR
                     GAEQVVLPELVKHPGMVFISNIHFDTTRAHVELANARAVDTVIPECYDLDKWLPFKGN
                     FDLNLLEEAIKEHKPENVAGIIMTITNNSAGGEPVSMENMKAVRKIADKYNITVLIDG
                     ARYAENAYFIQQREKGYEHKTIREIAREQFDQADIFMMSSKKDGLVNIGGLIAIRNDV
                     DLFLRCQTRIVPMEGFPTYGGLAGRDMDALAVGLNEALEEDYLAYRIAEIEYLGERLQ
                     EQGIPIQHPTGGHAVFVDCGAICPQIPYDQFPAQAVCNELYIEGGVRGVEIGSLLLGR
                     DPDTGKNLKAGKEFLRLAIPRRTYTLEHFDYVSEAFGKVYRKRAEIKGLEFVYEPPIL
                     RHFNAKFKPVE"
     gene            6145..7374
                     /gene="arcA"
                     /locus_tag="PLP02_02005"
     CDS             6145..7374
                     /gene="arcA"
                     /locus_tag="PLP02_02005"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HPF42268.1"
                     /translation="MFVDTLHVTSETGTLKKVLLHRPGRELENLTPIWLDQLLFDDIP
                     WLDLARKEHARFADIFRKNGVEVLYLADLVSEALDSEASIKENFIAQFLDEANVTSDT
                     LKQVLHDYLSSFKNTKDMVEQTMAGIRKSEVPNFVKRTLSDYIRDYPFVTDPMPNLYF
                     TRDPFTVIGNGVAISHMYTQTRSRETIYGDYIFKYHPVYGMGRNPMYYQRNDLSTIEG
                     GDILVLNEKTLAVGVSQRTHPAAIEKLAKNLFYNNPTKFETIIAFDIPKSRSFMHLDT
                     VFTQVDIDKFLVHQEVKRTMKAYELTKNKNREGKLTVIPIEQKLDAILEKYIGIPVTL
                     IPCGGDDVVSSAREQWNDGANTIVLRPGEVIVYERNHITNETLKRHGVKVHVTPSSEL
                     SRGRGGPRCMSMPFFRE"
     gene            7485..8159
                     /locus_tag="PLP02_02010"
     CDS             7485..8159
                     /locus_tag="PLP02_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42269.1"
                     /translation="MSHVEVVGVSKDYWNQNISTKALNNVSLKIEKGDFCLIVGPSGS
                     GKSTLLYVMSGLTEPSSGDVQISGKSIKGISSEKMAEIRREEFGFVFQFFNLIPNLNV
                     LDNVMLPQVIGHHIDENRALEVLRIVGMAEFKDRFPDQLSGGMRQKVAIARAIVNEPG
                     LLFADEPTGNLDQKSGREIMELLQKLNHTNGITVVVVTHNPDHLIYGSRKVELLDGVV
                     TSDEKL"
     gene            8146..10617
                     /locus_tag="PLP02_02015"
     CDS             8146..10617
                     /locus_tag="PLP02_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42270.1"
                     /translation="MKSFKFKLRFAFSNIWKNIGNSLSFVLSFALVALFALLLSNVFS
                     VSRQALYYQSIEKYGDIDVVVTYDEDASSRIVNRRSMTEEYAVYVDKAYSFFNLSVVA
                     ETASDTFYVDVMSANTSEFELLVDTELGYVGDTDAVITETMAKKYDLQVGNDLTMYLL
                     GQTYTYEIIRIIPDEGLFCGNSVFVDKTKILDEFLGLPQLDNLGNTLYICLENDIECD
                     DFIAMIEANPQYSDYLIYASVDQNLINTRATRMSSILFGIGVLLAVALVMVMHSLFPL
                     IAAKLRSQYGTIVSLGGSGKFILPVWLVELAVYFLIGGLIGLFSSFLIFQYVAHTYGI
                     EHMIKLDFLMSFIAFLVLGVLMLAEGYVNYRKIVRTDPISLSFNKRYEKQHLYGLLIP
                     ITFLVVILVMVFKPFTSAWNSLAIVVFSLMFAFLSISATVSNISRSIKKHRPKNLFGY
                     FNNTYLGDNIHIHRSLKTLLISILIIVMSLSVREFIFDETAKANEAFLFDYALVNIFD
                     YSDDLKQEILDDYPVEMADEALFYQNVYINFSEEENQETNVKISYFVSIDPDMFNSYF
                     RFGFEGINTLSSLPNDRVYVVIPKNYSQTYNLSSGDTVELDIGPSHDPVTAVIAGFLD
                     TNYEGIVYSNLMMVAGHETDYNVNSMLIRAENAEDIFRELSIDYGEEMYYVLDMDEVI
                     GEVESEVTTAGNLFLVLTAVLVACFMIIIVNDTLLVYYSLEKDLARLKAIGVGKKDIM
                     FSLLTEAALTMAIVCVFVLLETMVLSAYLPKVMLFFHYYKEVVPSGFTLMLGLVLGFA
                     SYVFGYFLYYRKIIHTDIMTEMRIE"
     gene            10628..12910
                     /locus_tag="PLP02_02020"
     CDS             10628..12910
                     /locus_tag="PLP02_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42271.1"
                     /translation="MKPKKLLVCLGVLSAIFFLAGCGKDPVEIDYDEFVATPTNLSIS
                     RADNELTWSPVENATGYVVYIDGEAESTETVTSFDFTSYIGDDVIVFRISALAPIGMK
                     DSGQSVSIAYNPNPAEERAAIISVLSNNGYGDWGTDDFIDALITKGVTAEDLLAFINA
                     YKQYEDDVESASGDPVGIMDAFEEFMSHVENIEAFVSALMSTKLMIIIQDEIDASQEQ
                     LDFYMSLSEEIRDLFYAEEIENLENNIIAMENAMLAIQDSFDYTVLSVTGSIEYLMTV
                     FTDMDTVLTSVTDIYQEGISGFEDFNPDEFIVIKDELVGVLRDNEPSMSDCVLLFETI
                     NAIGEVFSGINLQSDDFASITVTQYAAKMVIGYQIAIEYIDSYDLDFFTTFKDLGLTY
                     AEDQPAMLEAEIEILYLKHVKAFKDDNQNLFDQYDEVFTPEEKQSIFGFYVSYLMEAS
                     NILQAFSGYTEYENEISSFLENLQNVGYETMDTISDMSEQCYDKMMNYFVKTDGEILR
                     LMAIADQFYVTYPDHVYVFGNYALDITYKSADELYMAILENQIKIIDHILGYLDAMTG
                     EISDDDIPALVDLIQTTIPYDSIAAIFDMDEGAMIKFETDIDTLITEQLPNVVELLDN
                     LGGYEKFYNVIGRLANRISGWDDYFANDTEDYVLIIYLLDYYGSFMNSGNQDLVNEIK
                     TGIFKTMKEPEIIDMLSSYVYGLDEGDINELENNFDLAFAFSLSEADVISRLDKNNLT
                     VADKARIDRYFDTIKDYFIS"
     gene            complement(12967..13839)
                     /locus_tag="PLP02_02025"
     CDS             complement(12967..13839)
                     /locus_tag="PLP02_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HPF42272.1"
                     /translation="MQDIPEEILSQIKSLQRGEITEYYVYTHIAKRTKDAGNRKILLK
                     IASQEKHHSDIWSQYLGRAAKPKKGLILYYRILTAIFGYTFALKTMEKREDKAKTDYH
                     EIAKYVQEAIAISQDEEDHEKELIGMLDEERLSYVGSMVLGLNDALVEISGTLAGLTF
                     ALANTRLISLSGLITGIAASLSMAASEYLSERADNNPKALKSSLYTGVAYVITVTLLI
                     LPYLLLSDPYIALGVMLGVVVFVIFFFNYYVSVAKDLPFGKRFGQMAIISIGVSAISF
                     GIGILVKLLLGVDI"
     gene            14116..14433
                     /locus_tag="PLP02_02030"
     CDS             14116..14433
                     /locus_tag="PLP02_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42273.1"
                     /translation="MEQWDIIIYFILIGVLLFVGKILKTKLPFLNRIVLPTALLGGLI
                     GLLLSSVVIPGSYIIDTEVMGPIVYHALALGFIALTLKQEKSKNKRKVWSTGMVITST
                     YAL"
     gene            14494..15498
                     /locus_tag="PLP02_02035"
     CDS             14494..15498
                     /locus_tag="PLP02_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HPF42274.1"
                     /translation="MLLPLGFGQGPGLAYSFGNMWSELLGNQGASLGMSYAFLGFVFG
                     GTVGVLCINILSRKKGVQKPTQYEDVSLYKTTIEVDTIKEVSVLDGLTVQVVIISVIY
                     GLVWGTLYLVKAPLMSLNTETSNIGSTIFGLMEGFNFIIGIGYALLYKIILNKLRKKN
                     KTLNFMVNDYVVSNIASLCFNYMIAGAVLAITADFLKDYGILLAVVSAVGGVATLFYL
                     RFITYRVYPNYKAEYFVGLFGMLCGVASTGIALLKGLDRDLESPVAEEMVLGSGTAIT
                     MALPLFAVLILPSLGYDKFGNPTANLSLYNNIALFGCLAFVLVMVGILLFRSRKKVTQ
                     "
     gene            15605..16624
                     /locus_tag="PLP02_02040"
     CDS             15605..16624
                     /locus_tag="PLP02_02040"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPF42275.1"
                     /translation="MQYRITEKGNLLNEKGRLATSGYATSLLLHYDRNEIAASPWRIK
                     EWDYYAVLTAKYGVAFVVSDLGYTAMVSAVFFDFVKKTQKSMTKLFWFTFGKMGLPSS
                     SEKGNVSCHQQGYDFDFIRNQDERLIKVTIKDFYPGKDLRADLTLKDMKDETMVIATP
                     WQEKPKAFYYNQKINCIPAYGSVLIGEESYAFKEGEAYGVLDWGRGVWTYKNTWYWSN
                     LSGIVDGHRVGFNIGYGFGDTSKATENMLFYDGVSHKLDQVTFEIQEPDFLKPWKFTD
                     NEGRLSLTMEPLVDRQDHTNMIIIKNMGHQVFGKFTGFIVLDDGKRIELKDMIGFAEK
                     ITNHY"
     gene            16635..16943
                     /locus_tag="PLP02_02045"
     CDS             16635..16943
                     /locus_tag="PLP02_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPF42276.1"
                     /translation="MTNKELYDLLTGDGFCTIELDGDDNRKYEMEQIGLLPMHGVTYA
                     IMNILKVDDTPVDKNDAGVVLFELDFDDETEEYFVATVEEDDVFEEVMTEFEKIPPTD
                     "
     gene            17026..17403
                     /locus_tag="PLP02_02050"
     CDS             17026..17403
                     /locus_tag="PLP02_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HPF42277.1"
                     /translation="MNIYYCKHCKRICYTYPVDSTLVCCGDEMTLLQPKTSDVGNEKH
                     LPVVERIPNDMIKVTIGSVLHPMLPEHFIQWIFIAQDDSYQIKTFKPGDTPVATFHIN
                     PDQDIKVYEFCNLHGLWMTEVKK"
     gene            17509..18348
                     /locus_tag="PLP02_02055"
     CDS             17509..18348
                     /locus_tag="PLP02_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPF42278.1"
                     /translation="MDILELLKYLLLGLIQGLAEVLPVSSTGQVELVRSLIGVDVEKE
                     LLFLILLNTGSFIVLLFAFRRQIGVLIRDFFRYIFSGKSHPESKPGFVYCIKLILATL
                     PAAVVGLFFRHKIDGFLLNYGTLFSGIGLLVTGTVLLYVGFGDYRHQNGKIGWLDSVL
                     IGAGQALALIPGISRSGMTTSIALKRGVDIDSALDFSFLMYLPVSFGGLVLLLLEASG
                     SGFSVPSPSHYVYYAVAFLGAAGFTYLAFRIIYNVFRSGKLKYFGYFCLATGILSLIV
                     YAF"
     gene            18395..19258
                     /locus_tag="PLP02_02060"
     CDS             18395..19258
                     /locus_tag="PLP02_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPF42279.1"
                     /translation="MSIVELIKYIFLGLIQGFTEVLPISSTGHVALFQIILNIDTDSG
                     LVFMAIVNFGSLLAVVWHFRLYIGRLIKNFFVFMFDKESRNETRDDFLYAIKIIIGVI
                     PAAFAGLFLTGYVNEIYKRYSLLLVGIGLLVTSTFLFIVRKGPERHVKQTLTFKDATI
                     IGLLQPFSIVPGLSRSGITVSGGLLRKASMDTSLSFSFMLYIPLSIGFACQSVYYMIV
                     QPTGHNLGFDPSDLYQYIYYGAAFLASLAATRIGLKYIFKWFRNGKLVIFAYYTLFLG
                     MIALVAGAVIL"
     gene            19360..20097
                     /locus_tag="PLP02_02065"
     CDS             19360..20097
                     /locus_tag="PLP02_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPF42280.1"
                     /translation="MEEFEKQETSTEEGMTFTELLRIVWDNFLLIVLITVWITALGFV
                     YTFAVVSPKYTAETAAMVQVDTDSGTSEQSAISIANYLMTTYKEFIVSRLVLESVIND
                     VDGISDMTVEQLANSVTVSSTSGAYIIYINVDNTSPELAQAIANQIVDNAIAIADDEA
                     NGFTFLQDKLKLVYSAALPEAPSSPNKPLNIIISFILGGVIALMVVFFKEIFNNKFQT
                     KEELEKVLNLRVIATVPGTIKERKLVD"
     gene            20098..20790
                     /locus_tag="PLP02_02070"
     CDS             20098..20790
                     /locus_tag="PLP02_02070"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HPF42281.1"
                     /translation="MEYFEYHNVVIESPNSREAEAYRKLELNIAMMSLDEKIQVIQST
                     SAIPEDGKTTTAINLAAVYAEKGEKVIIVDFDLRRPKIHRAFKKPNEKGFYDYVNGDI
                     DFHDIIVHDESGIDLILTGKHLSFPHIVLESHKASDLIAKLRKEYDYIIVDTPPVLSV
                     TDSLIVSKYADGVLYVVAYNKTKKDDAKAGLLQLQKNHARVLGSVFANIDIKKFKGYH
                     GYDYYSHQDKTS"
     gene            20805..21497
                     /locus_tag="PLP02_02075"
     CDS             20805..21497
                     /locus_tag="PLP02_02075"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42282.1"
                     /translation="MIDIHSHVLPFVDDGSDNMEMSLAMIREASEAGVTDLFLTPHYM
                     RLRDYLSNASENREIFSELTNKVKQEGISIKLYLGNEIYYSLDILDALRTGTIIPLGN
                     SNKVLVEFSLFEEDEEITEAIHNIKSLGYVPIIAHPERYPYISKLSDYEIIKQMGALI
                     QLNASSIIGQRDKLAHKKAVAIIKAGLADFIASDQHVPGKNHLEEARNIINRKFGLPV
                     AKALFENTVVLE"
     gene            21746..22435
                     /locus_tag="PLP02_02080"
     CDS             21746..22435
                     /locus_tag="PLP02_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF42283.1"
                     /translation="MKSFYVLVADDEFRIRKLLADFLVREGYKVLEASDGEEAIDLIF
                     ESKIKIDLVILDVMMPKYDGWFVLERIREFSTVPVIMLTARSDESDQLKGFKLGADDY
                     VTKPFSPSILMARINKLIKRDNVETTELVFGKIRMSLASREVFVDENKVELTPKEFEL
                     LKYFIDNKGIALSRDKILNAVWNYDYFGDLRTVDTHIKQLRSKLGDYAHYISTVRSIG
                     YRLDIENENVN"
     gene            22419..23966
                     /locus_tag="PLP02_02085"
     CDS             22419..23966
                     /locus_tag="PLP02_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF42284.1"
                     /translation="MRTSIKSRLFLITYGIILCFILGLIVLNNTYLEKYYMNYRENTL
                     LNAFTEVKTIDLSEDTYDSLLFDVEENYNISIQVLKERGIQPNIDNLPSPYLRLYGNT
                     MSISPGIISQIMLQLNLLDDGYSSSSSVDVGDGYVAFVQNIIPTSSTNGDDVQLLSLC
                     AATDSGDGLLMYYILTVTVQSIRDSISVFNTFTIIIGFIFMIISGIFTYLISYRFTNP
                     ILEINQVARQISNLDFGSKVEVSSNDEIGDLGNSINLMSTQLENAISKLKAANEKLEN
                     DIQLKEKIDSMRKEFIASASHELKTPISLILGYAEALKLPGIDKDTSDDYLNIIMDES
                     NKMNKLVMEMLKISQLESGFSEFTVSDFSVKDMTDETARLFSIIFEESKINVTIDVLD
                     REVSSDYDQLQTVLSNFISNAIHHVDKNKKIRIYSEETNEGNLRLVVFNTGLAIAESD
                     QDRVWESFYKVDKARTRSYGGQGLGLSIVRNTLDNIGYGYGVKNVEGGVEFYLEIPAK
                     DIKSNNQ"
     gene            24093..25955
                     /locus_tag="PLP02_02090"
     CDS             24093..25955
                     /locus_tag="PLP02_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPF42285.1"
                     /translation="MRRIKNESMGDYMRKIRIIQYIFVDAVCIVFTYSLTFLALKFDG
                     ASNFDIETAFKVLPLVLVFKLVAFYLMKVYRLVMDNAGIEEVFRIFIAVITTNIVITL
                     ASLFIPNYNFIPEVSMIFTTAFEGIILSVPRVIKRLTNLVSRKHPVGGKRTLLVGAGA
                     GGKLVLNEIRNNPELNNVPIAFVDDDPLKIGKIMSGCPILGPIDKIPKFIDIYQIEEV
                     IICIANIDPKWFQEIIQTAAERPVRIKRIPKFKELKKDTPKKFLEINVEDLLSRDVVE
                     LDSSGISEFISGKRVIVTGAGGSIGSELVRQIVDMRPSELLMLDIYENGVYDIQMELN
                     HKYATSDEEPVQRKVLIGSVYNYNRMNAIFSAFKPQLIFHAAAYKHVPLMEDSPIEAV
                     RTNVIGTYNICKLASLYNVEKVVLVSSDKAVRPTNVMGATKRFAEMIMQYFNSVSSTK
                     YSAVRFGNVLGSNGSVVPLFKKQIENGGPLTVTDERIIRYFMTIPEAVGLILQSGTFA
                     KGGEIFILDMGDPVKIIDLAEKMIMLSGRRPYIDIDISITGLRPGEKMYEELLVDKTK
                     DITKTANKKIFIDRGSEIMPIEEEIRDITGKFETLNSVEIKEMIKSMVSTYTPK"
     gene            26063..27445
                     /gene="rlmD"
                     /locus_tag="PLP02_02095"
     CDS             26063..27445
                     /gene="rlmD"
                     /locus_tag="PLP02_02095"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPF42286.1"
                     /translation="MFSLENGSKVKLEIRKMGINGEGIGYFNRLAIFVPRAITKEIVN
                     CEIVELYDKYAVAKLEEVVKPSNRRVEPPCKFYEQCGGCQLQHVEYKEQLKAKLSLLK
                     QSLSRYTSLDIASLKIRKALGLANNYHYRNKSQRPFKNTNFGLALGLFRQGTNQFVYV
                     DTCMTEDEHVNQINQEALTVLRKYHVMASDQMNKEGILLNLVTRYLASTETASVTFIV
                     TDFDPVLKEVAKDLMAKNPSVVSVTYSVNKKNNPMMFGPAVELLAGKPYITDLFSNMK
                     IRISPDAFHQLNTAQMKVLYDEIVKAAELEGKETVIDAYSGIGMTSLMMARKAKRVIG
                     IDYSPASIADATKNATANGIKNVSFICDHVEGALPKLIANGVKPDILVLDPPRAGLPE
                     EIVKLIVKTKIKKIIYVSCNPSTLAKNIAGLESKYEVCYIQPLDMFPHTANVESVTLL
                     KLKESGGANR"
     gene            27442..28458
                     /locus_tag="PLP02_02100"
     CDS             27442..28458
                     /locus_tag="PLP02_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPF42287.1"
                     /translation="MRIREYVMPKSLIYTGEHPEVKTKIVHYQYDENKVEITDNFVSL
                     PGKKHYIQVIGLKEIDKVKTIREFYSINPLILEDVFNVKQRTKVEMHDDFLFSSLHLE
                     YQDGEEIKHDYLSIFMNKDALITFHETEPAYLAALPGLFQKNEELRKRGIDYLYYQIL
                     DIVTDNHLDIYDTLEKRANDFEEEILENEKIDQESFYLTRKQFLKLKEAVSPLMSELR
                     VAMGSATAGLLFSKDTEPYFQDLRDHLQRLDDQLNTAREEIRHLLDLMMNNQSYKMNQ
                     IMTTLTLFSAIFIPLTFITGIFGMNFTYFGILDYKYAIPAFTAICVLLAVGMLIFFKR
                     KKWF"
     gene            28486..30153
                     /locus_tag="PLP02_02105"
     CDS             28486..30153
                     /locus_tag="PLP02_02105"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPF42288.1"
                     /translation="MDSDLQIAQKAKMETITKIAGKLGLKDSDIELYGNYKAKINMEA
                     VKSDRFQKNGKVILVTAINPTPAGEGKSTTTIGLGDALNRLGKNTVICLREPSLGPVM
                     GVKGGAAGGGYAQVVPMEDINLHFTGDIHAVTAANNLISAVIDNHLYFGNQLNIDPER
                     IVWKRAMDMNDRALRRIQVGLSSKKETPRYDGFDISVASEIMAVLCLARDIDDLKKRI
                     ARIVVAYDKSGNPVTVSDLKAAGAATMLLKDAIKPNLVQTLENNPVLIHGGPFANIAH
                     GCNSLIATDFARRAGDYVVTEAGFGADLGMEKFLDIKSRLLGVLPSAIVLVASIRALK
                     HHGGVEKDKLAEENVEALAKGIVNLEKHIENIGFYKIPFVVALNRFLSDSQAEMDYMM
                     NWAKENNCPMALSDVFANGSAGGMELAKMVIETIENKSSQHSGLLYDVDLSIKEKIER
                     IAKKVYGAANVEYSEAAEKAIETYDAFGWNKMPICMAKTPLSLSDDPKKIGRPENFTI
                     TIRDMKPSIGAGFLVALTGDVMTMPGLPRNGAFENMDVIDGKIIGLF"
     gene            30170..30595
                     /locus_tag="PLP02_02110"
     CDS             30170..30595
                     /locus_tag="PLP02_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42289.1"
                     /translation="MKKEISDTMKKLRDLGLKIAKTAEKGYQQAKSSVESTILEDSLR
                     RRFNLENPYKFQIRRDNSVSDLIEGIMPRNAKRYDEDDMFVFYGDKESNNLTKGDLIK
                     DLSSNADYRIKDLIEVEVPVELDGKTYQVIGTAALCDLL"
     gene            30601..31194
                     /locus_tag="PLP02_02115"
     CDS             30601..31194
                     /locus_tag="PLP02_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF42290.1"
                     /translation="MANYQDEFIDLYRKHIRRPGSEELLQYLLSDQSDFFTAPASTRF
                     HLSHEGGLVEHSLNVYHCLRDYLGRERVTDEYKCQPSEESVAIVGLLHDLCKVNVYRK
                     SFRNVKDANGKWNQVPSFDYRDDLPYGHGEKSVYIISGFMRLTREEAFAIRYHMGFSA
                     DMENKADVSRSFELFPLALCLSIADMEATFFLESSEE"
     gene            31234..32619
                     /locus_tag="PLP02_02120"
     CDS             31234..32619
                     /locus_tag="PLP02_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPF42291.1"
                     /translation="MARIYNLTEGSIIKKLFYVALPVLLLSLSQMAYNLTDIFWVGHV
                     ELIGIVESQAISAVGTAAYITWFAFGIIMIGRIGTSVKVAHSVGENKTDRLAGYAENG
                     LILQAILGLFFSVVVVVFRRQIISIFNIGNENVVEYTVNYLAIVGSFLFVQFLTYGFV
                     AINDGVGKTMTNFLIMLIGIVVNMILDPLFILVLRMGVSGAALATVIGQIITLVAFVF
                     LYAKKNKMFRFRLSAFRKKYLLEIVKIGLPTGLQSMLFTSISIFIARMVYEYGEYVVA
                     AQRVGVQIEQLTWMIAGGFQTALTVFVAQNFGARLHGRIRKGFGIMSGLLLPYAAAVA
                     LCLYFFSDFLLGIFISDPVSLSYGVAYLKIISLAQIFMMMEAIGTGLYNGVGKTYIPS
                     IIGIVGNVLRIPLAIELVKVIDENGIWWSLNISDMFKGAILYAGSLILLAFLERIKLN
                     KKQNRQLVEET"
     gene            32729..33664
                     /locus_tag="PLP02_02125"
     CDS             32729..33664
                     /locus_tag="PLP02_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF42292.1"
                     /translation="MDWYWWVLIFLALIAIYLVNIGYLVAKMLFHPKRMTLSETRKRE
                     LEKSPEILKATKDWRFSMYQIESRHKYPLQLYFLPAEIPTKRYIVIAHGYTYTSHGGW
                     KYATFMRNYGFNVILYDERYHGASGGKNCTMGYYEKDDLYDIISDTYKRFGNDIYLGT
                     YGESMGGAIVLMEQSGDDRVKFVVADCPFADLSLLLKYLIRNKVHLPMYPVIWIVDFF
                     FRLFTKTSIFRISPIEEVRKAKVPILFMHGGEDAFIPPIHSELLFAACPTGKDLFIAG
                     SHSRHTDACRNETDKYKKTLRNFMVNIVHADIDGR"
     gene            33669..35105
                     /locus_tag="PLP02_02130"
     CDS             33669..35105
                     /locus_tag="PLP02_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF42293.1"
                     /translation="MFDVIIIGAGVVGANIAAEMARYDLKIAVLEKHSDVCEETSKAN
                     SGIAHSGYDALPGTLKAKYNVIGNKMMKDFCERLQIPFKQNGSLIISLTDEGIPKLEE
                     LYDQGIKNGVEQLRIIGKEEIFSLEPNLNPEVKHALYAPTGGIVDPFQLTIGPAEIAC
                     QNGTKFFFDTEVTNIRKENGHFIVTTNKGDFQTKAIVNAAGVFSDDINNWISEHKKKI
                     MPRKGEYCLFDKEVGDMVHSTIFQLPTPKGKGVLVTPTAEGNLLVGPNSLGKYDKEDT
                     MTSAEGLDYVLETASQSVAKVPTKFIITGFAGLRASEEGGDFVLGEAEDVPGFFNALG
                     IESPGLTAAPAIAIDMAKWVATYLHANLRENYIDKREKLVRFEQLGNAERQKLFLSDP
                     EYGHVICRCELVTLAEIKNAIHRPLGATTVDGIKRRTRAGSGRCQGGFCSPRVLEILA
                     QELKKDPREIEKFSEKSTYLVGFDKDML"
     gene            35116..36375
                     /locus_tag="PLP02_02135"
     CDS             35116..36375
                     /locus_tag="PLP02_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF42294.1"
                     /translation="MFEKDLVIIGGGPAGLAAAVAAHKNGVKDILIIERENHLGGILN
                     QCIHNGFGLHTFKEELTGPEYAKRYVDQVLEEKIPYILDTMVIDLDHDKKITAINETD
                     GIMEIKAKSIILAMGCRERPRGALNIPGYRPSGIFSAGTAQKFVNIDGYLPGRNIVIL
                     GSGDIGLIMARRLTLEGANVKMVLEIMPYSGGLKRNIVQCLNDYNIPLRLSHTVTNIY
                     GRDRVEGVTVSAVDENMKPIPGSEEYVECDTLLLSVGLIPENELSRKAGVEISQKTQG
                     PVVSNSLETSIPGIFACGNVLHVHDLVDYVSMEAEAAGEYAAKYLEGLPAVKRQTVIQ
                     TGNGVRYTVPSYVDKGSLTGSLTVRFRVSGVYENKYISVYFDTKRLYHKQKRVLTPGE
                     MEQVLLPKIWFDNVSDLKTITIVVEEE"
     gene            36381..36746
                     /locus_tag="PLP02_02140"
     CDS             36381..36746
                     /locus_tag="PLP02_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPF42295.1"
                     /translation="MAKEKTLTCINCPLGCQLSVKIDGDKIEVSGFSCPRGEKYAKEE
                     VLHPVRVITSTIPVVNGEVPMVSIKTKGEIPKDKIMECMISLKGLKAKAPVHEGDVIL
                     ANVCNTGVDIIATKEVKAI"
     gene            36743..37204
                     /locus_tag="PLP02_02145"
     CDS             36743..37204
                     /locus_tag="PLP02_02145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPF42296.1"
                     /translation="MKKIFVIKTKYRNFQIVIEDGFLLSIDTTSENETPPELLDGPAK
                     IVLRQLEEYFSKNRKNFDIPYKMKGTLFQQDVWKALHQIPYGKTASYSLIAEMSGHKK
                     AIRAVGTALKNNPLPIVIPCHRIIRKNGLTGNYNGGADMKKYLLELEKNGS"
     gene            37333..37569
                     /locus_tag="PLP02_02150"
     CDS             37333..37569
                     /locus_tag="PLP02_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPF42297.1"
                     /translation="MTLKDVKPGEKCVIKNIFVNNLRKRIIDMGLTTGTIVAVKRMAP
                     LGDPMEIRVRGYSLTLRKAEARMIDVELVEGNGK"
     gene            37566..>39658
                     /gene="feoB"
                     /locus_tag="PLP02_02155"
     CDS             37566..>39658
                     /gene="feoB"
                     /locus_tag="PLP02_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPF42298.1"
                     /translation="MKKIALLGNPNCGKTTLFNSLTGSNQSVGNWPGVTVEKKSGFLK
                     RDNFMAEIIDLPGIYSLSTISLEEEIAARFVYGKNMDLIINIVDASNLERNLFLTYQL
                     LDAGVPIIVALNCMDIIEKQEDFIDAFAMEKELGVPVVPIVACRKNGTEELIDKIINF
                     DYTVSSTSHLYGEKVEKTIKEFAEALGGDRFAAIRFFEDGLKSLDLKEIKLDKEETAK
                     LLDSYAKNRKEYDIDFDMVIPNDRYDQIIKLCKKVYRKNPLETESKTDKIDRVLTNRW
                     LGLPIFAAIMFGVFYLAFGPVTSYITVWFVKGLDWLFSYIEDFLASAGAAAWVSSMLE
                     NGIFGGLSAVLGFLPQLVVLFLFLSILEDSGYMARAAFIMDRALRRFGLSGKSFIPML
                     IGFGCSVPAMASTRTLDRDDDRKVTTMIIPFVSCGAKAPIYGVFAGALFFGYSYLIVF
                     SMYILGLLVAIMSAILFKKTIFKGTSANYLMELPEYRMPTFKNTMLHTWERSKAFLIK
                     AGTILLGAFIIIWFLSYFGFKDGVFGLLDVSDLEYSFLGYFGKFILPVFRPIGFTSWQ
                     PTVAILTGIVAKESVVGTLGILYGVTGNAIENGSLLYESIRSAFTPAQAYAFMTFALL
                     ASPCVAALSAMRKELASKKWFWFTVFYELIVAYLVSLIIYQVGSLGAGGVITAIVAIT
                     AALIVFFTIKRIIRT"
BASE COUNT        11829 a         7912 c         9171 g        10746 t
ORIGIN      
        1 catcagtaag acgtgtgaaa gattggtttt tagatgatgc agtcaatgga gacgttgatg
       61 cacaatatct tattgctcat gtctataata gttatacaag tcaacaagaa catcattatt
      121 ggctgaaaaa agccgcagat caaggacatt cggcagctca gtataagttt gggatagaaa
      181 acaaagatga aaatccaagt gattatatcg aatatatcaa gttagcatca aaaagtaatg
      241 tatatgctca gatagaacta ggatacaact atgctcatgg tcagtatgta tgcaagagct
      301 atttggatgc atacaattta tatcaaaaag ctgcagataa tatgaagaaa atcgaagata
      361 ttcgtgagtt aagaataatt aataatatta aaatccgtta caacgcagca aatgatgaag
      421 ctgaacaatt agctctaaat gatgatgttg atgctcaatt gtatatgggc tgtttgtatc
      481 aatatggttt tgaaataaaa cgtaacaaag aaaaggcggt atactggtat gaactggcca
      541 atacaaatgg aagtgtggaa gcttctgagc aattgattat tattgataat gaaatgatat
      601 aacctatgaa tcaaaaaatt gaagcatgtg cttagagaag tgaagtataa acttaactaa
      661 gatcatgaac tttacaaagg ggttatatgt tcacctgtac aactgaaatg atttatgaga
      721 catacagtga caatacaata ggtatttctg tcagatggtt tgggtgaata gcatatacat
      781 acataaaaat aaatgtataa cagaagtgct tagtataata catttataga aaagaggggt
      841 attatgaatc aaaacgtttt gaacgaagcg attaagcgat tcgagtgcag cgattatgaa
      901 aaagctatat atctatttaa aagtatagca gatgatcatg ttttagcaat gtattatcta
      961 ggttcaatgt atcgcatggg tttgggggtt aaggaagacc acaaagaggg attcaaacta
     1021 ttcttgaaag cggctaaatt aggacatgct gaatcacagt ttttagttgg gtgcgcttat
     1081 actgggagtg gtatttataa tcatagtgaa acagagcttg tgagatttga tatagaaaca
     1141 attttagaaa aatcggtgaa agacaactct gtatggcaag attcaatacc tttctatcat
     1201 cttgatggaa ttggcgtcga accgaataat aaagaagcaa ttaaatggtt atctaaagca
     1261 gcagaacaga actatgttaa cgcacaaatt gtattaggtg atatatataa ctggggagtc
     1321 atcgttgata atgataaaga aaaagcaaaa caatggtatg aaaaagctgc aagcaatcaa
     1381 aattccaaag cagtgagaga gctagcattt ttcttttcca gaaataaaaa tgatttagct
     1441 aataagataa agatgctgat aaaagcacat gctttggggg ataatcttgc agcatatttt
     1501 cttgggaaaa catatgaaag agatattaaa gaagaaggac atattgaaca agcggtttac
     1561 tggtataaga ttggtgcaga aacacaaaat tgttataatt cacaacttga acttggtgag
     1621 tttgcactta atggaatagg acaaaaaaaa gatattaaac aagcagtaac ttggtataaa
     1681 aaagcaatag agacatttaa tttgatacat cattatagcc attatggtca agcaaatgaa
     1741 agattaattg acctctatga aaaagggtat aaggatttaa ttaccgaaaa cgaatacata
     1801 gaatacctca aaggtgcagt agaaagttct aaggattctg caagatataa attaagagag
     1861 tactacaaaa aaggttatga tgtaggtgaa gaaaacaaaa agttctttga attattagat
     1921 agtgctcagc aaggtgataa aaatgctcaa ataaagttcg gttatctata tatagtggat
     1981 aagttcattg atgaatcaac aataagaatt gtgaaagatt ggtttttaaa tgatgcagtc
     2041 aatggagacg ttgatgcaca ataccttatt gccaatgtct ataatagtta tactagtcaa
     2101 caagaacatc attattggtt gaagaaggct gccgatcaag ggcactcagc agctcagtat
     2161 aagtttggga tagaaaacaa ggatgaaaat ccaagtgact atatccaata cattatgctg
     2221 gcatcagaaa gtaatgtcga tgcacagatt gaattaggat ataactacgc tcatggtaag
     2281 tatgtatgca agaactattt ggatgcatac aatttatatc aaaaagctgc ggataatatg
     2341 aaagaaattg atgatatcga agagttaaga acaattaata atattaaaat ccgttacaac
     2401 gcagcaaatg atgaagcaga acaattagct ctaaatggtg atgttgatgc tcaattgtat
     2461 atgggctgtt tatatcaata tggttttgaa ataaaacgca acaaagacaa agcagtatac
     2521 tggtatgaaa tggcaaaaaa acaaggtagt aatgaagcgc agatacaact caatctgttg
     2581 tatatggaat tggaatagaa tatggatgag aaacgattaa agaaagtcct tatcgaaata
     2641 aaaaatggac taggcttaga ttatgcacta cataaaggat ttgtaagtcc aacatgtaca
     2701 gctaagaaac ttgatgtatt atatggggat aactcaattg gcatatatgt cagatggttt
     2761 ggaaaagatc aagagcaacc acaaattaga aacatggata aactatacat taatcatgat
     2821 tttggaagcg aaaacagtga ttttagaaaa agtgaaatag tcaacatact ttctaaatat
     2881 tatgacgttg attgggatta ctcagataaa aaatcaattc taattaaaga aaaaggagaa
     2941 tcgaaagatg cttgaattgt tgatagtact agttattata attgcaatta tattgttatg
     3001 catactaata tttatatgga atcaattaaa caacacagaa tctagactag tgaaaataat
     3061 agaggataga atcagattaa acaatgttca ttatgaactg gatagacttg actttccaat
     3121 tgttgaagat gaaccattgg attaaaaatg caataaaaat ataaaataga aagaaggtga
     3181 aacaatgcat acttatgaag taacgataat attaaaagga aacaaaagaa ttgttgatac
     3241 agttaaggca agcacaaagc aaaatgcgag agcagtcatg gaagctagat atcctggtgc
     3301 caatgtaaga acacctcaaa aaattgacta gttgtcgtca tgcaagaaaa ttaaaccaag
     3361 catttaatgt tgcttccgta agagttcgtt taatcaaata atgagtgtgc taatatatat
     3421 aagttaaatt actctcatga gtattaattt gagaaaaaaa ccaaaaaaat atgttacttt
     3481 ggtattgtag atggttttca accatgtaaa ggtaaacata ttttagcgga ttttgatgtt
     3541 tctttaatgc acacaaggtc gattttgcaa gcaatatagt tttttcaacg catatcgaaa
     3601 gcgtttcgtt gttgacattg aaataagatt cttgatcata aaggattcac atgactttta
     3661 gtgagaaata tgaatgttgt ttacattttg tatttcgatt ttcccttgtt gaatacagaa
     3721 agtgatgata taattacatc aattaaatca taggaggaaa atataatgcc attttgcccg
     3781 aaatgcggtg ccgaagtaaa agaaaatcaa gatgtttgct tgtcatgcgg aaccgccatt
     3841 agtaagaacg ctacatcaat tgtttctgaa gaaggatcaa caaccggatg gggagttttg
     3901 ggatttttct ttccccttat tggccttatt ctatacttgg tctggaagac tgacaaaccg
     3961 aaagctgcca agtctgccgg aaaaggcgct ttgatcggtg tcatagccgg atttgttttg
     4021 ggaattctcg aagcgataat tgtaatagca gcaggaatct aatcaataag aaaagccata
     4081 caattatttt gaaggatgtg atatttaatg ccatattgct caaattgcgg agccgaactc
     4141 aaagacggtc agcaagtctg cctgtcctgt ggtacctttg ttaaaaccaa caaggcgaat
     4201 ccaaaagcca ttgatgacgg gaaaacggat gccggatttt tcctgatcgg atttttcgtt
     4261 ccgttggcag gattgattct ttatttgctt tggcgtgaag aaaagccaaa agaaggacgt
     4321 tcagccggaa agggagcctt ggttagtgtg atcgtgtccg ctgcccttgg aattctttgg
     4381 atcgtcatta tgttgctcgt atttcctttt gcatttggaa tttaacaagc ataacgtata
     4441 tttggaaaag cattctgcga agaatgcttt tttatttttc tgaaaagaaa acaattgaaa
     4501 tgcaatgaaa aagacgatgt aagcgaaaag gcttacatcg tggtgaaaaa agcaaccttg
     4561 tttatgttat aatagtaatg cgaaaaggaa ttatctttct tcccctggag ccgctttttc
     4621 aatgacatgg atcttgccgg gcgaggaaaa caaaacgcca aagcgtaaat catcggattg
     4681 attacggctt ttttcgattg ccggaaccct gatgaaatca ataatacctc aaaatgaaag
     4741 gaagcaataa cgtgcagaaa aaaatcattg tcccgcctta caaaatcaag atggtcgaac
     4801 caatccacat catcgataaa aaaacgagga tggaaagatt ggaaaaagcc ggatacaatc
     4861 cgttcaaact aagaagcgaa gatgtattca tcgatttgct tacagattcc ggaaccggag
     4921 ccatgagtca attccaatgg gcggctctga tgcaaggcga cgaggcatat gccggatcgt
     4981 ccagctttta ccgtttggaa aaaacagtca aggaaatcac cggatacgaa tatgtgatgc
     5041 ccgcccatca gggaagagga gctgaacagg ttgtattacc ggaactcgtc aaacatcccg
     5101 ggatggtatt catcagcaat atccattttg acactaccag agcgcatgtg gaacttgcaa
     5161 atgccagggc tgtcgatacg gtcattcccg aatgttacga tcttgacaag tggttgccat
     5221 tcaaaggcaa tttcgatctg aatcttttgg aagaagccat aaaggaacat aagccggaaa
     5281 atgttgccgg cataattatg acgattacca acaactcggc cggcggcgaa ccggtttcca
     5341 tggaaaacat gaaggccgta agaaaaatcg ctgacaaata caacataacg gttttgatcg
     5401 acggagccag atacgccgag aacgcttatt tcatccagca acgcgaaaaa ggatatgaac
     5461 acaaaacgat cagggaaatc gcccgagaac aatttgatca ggccgatatt ttcatgatga
     5521 gttccaaaaa agacggtcta gtcaatatcg gcggcttgat tgcaatcaga aacgatgtcg
     5581 atttgttcct ccgttgccag accagaatcg tgcctatgga aggttttccc acatatggtg
     5641 gcttggccgg cagagacatg gacgccttgg cggtcggatt gaacgaagcc ttggaagaag
     5701 attatctagc ctacagaatc gcggaaatcg aatatctagg agaacgtctg caggagcaag
     5761 gaatcccgat ccagcatcct accggcggac atgcggtttt tgttgattgc ggcgccatat
     5821 gtccccagat tccttatgat cagtttccgg cccaagccgt ttgcaacgaa ctgtatatcg
     5881 agggcggcgt tcgcggggtc gaaataggtt cattgttatt gggacgcgat cccgataccg
     5941 gaaaaaatct gaaagcgggg aaggaattcc ttcgtttggc cataccgaga agaacatata
     6001 ctttggaaca tttcgattat gttagcgagg cgttcggcaa ggtatacagg aaaagagcgg
     6061 aaatcaaagg acttgaattt gtctatgaac caccaatttt gagacatttc aacgctaagt
     6121 tcaaaccggt ggaataaggg aggtattttt gtggatacat tgcatgtgac gagcgaaacc
     6181 ggaacattaa agaaggttct tttgcatcgt ccaggcaggg aacttgagaa tttgactccg
     6241 atatggttgg accaattgtt gttcgatgac ataccctggt tggacctggc gagaaaggaa
     6301 cacgcccggt tcgcggatat attccggaaa aacggggtgg aagtcttgta tcttgccgac
     6361 cttgtcagcg aagcccttga tagcgaagcg tcgatcaagg aaaatttcat cgcccagttt
     6421 ttggatgaag ccaatgtgac aagcgacact ttgaaacaag tattgcatga ttatctttct
     6481 tcatttaaga ataccaagga tatggttgaa caaacgatgg ccggaatccg taaatcggag
     6541 gttcccaatt tcgtcaaacg cacgttgtcg gattatatcc gcgattatcc gttcgtcacc
     6601 gatccgatgc cgaatcttta tttcaccagg gatccattta ccgtgatcgg aaacggggtt
     6661 gccatcagcc acatgtatac ccagacaagg agccgagaaa ccatttacgg cgattatatt
     6721 ttcaaatatc atccggttta cggaatggga agaaatccaa tgtactatca aagaaacgat
     6781 ctgtcgacga tagaaggcgg agacattttg gttctgaatg aaaaaacact ggccgtcgga
     6841 gttagccaaa ggacgcatcc ggccgccatc gaaaaactgg cgaagaatct tttctacaat
     6901 aatccgacca aatttgaaac catcatcgct ttcgacatac cgaaaagtcg ttcgttcatg
     6961 catttagata cggtattcac acaagtcgat atcgacaaat ttctggttca tcaggaagtc
     7021 aaaagaacca tgaaggcata tgaacttaca aaaaacaaga atcgagaagg caaactaaca
     7081 gtcatcccga tcgaacagaa acttgatgcg attttggaaa aatacatcgg gattcccgtc
     7141 accttgattc cctgcggcgg cgacgatgtg gtgtcatccg ccagagaaca atggaacgac
     7201 ggagccaaca ccatcgtctt gcgtccggga gaagtgatag tatacgaacg aaatcatatc
     7261 acgaacgaaa ccctgaaaag acacggagtc aaagttcatg taactccttc cagcgaattg
     7321 tcccgcggga ggggaggacc acgttgtatg agcatgcctt tcttccgtga gtgagcagat
     7381 aatacaaagt ccggagttcc ggactttttt atctccgtat tgtttgaacg ctgaaagcat
     7441 ggtatcatat ctttgaactg caggttgcat cacgggaggt cattatgtcg cacgtggaag
     7501 tagtgggtgt atcaaaggat tactggaatc agaatatctc caccaaagcc ttgaacaacg
     7561 tttccttaaa aatcgaaaaa ggtgattttt gtttgattgt cgggccatca ggatcaggga
     7621 agtcaacttt gctttatgtc atgagtggac taacggaacc gtcatccgga gatgtgcaga
     7681 ttagcggcaa aagcatcaaa ggaatatcaa gcgaaaaaat ggctgaaatc agaagagaag
     7741 aatttggctt cgtctttcag ttcttcaatt tgattcccaa tttgaacgtg cttgacaatg
     7801 tgatgttgcc acaagtgatc ggacatcaca ttgatgagaa cagagcttta gaggtattaa
     7861 ggatcgtagg aatggccgaa ttcaaggata gattcccgga ccagttatcc ggcggcatgc
     7921 gccaaaaagt tgccattgcc agggcgatcg tcaatgaacc cgggcttttg tttgcggacg
     7981 aaccgaccgg aaatctcgat cagaaaagcg gacgcgaaat aatggagctt ttgcagaaac
     8041 ttaatcatac gaacgggatc acagtggtgg ttgtcacgca caatcccgac caccttattt
     8101 acggatccag gaaagtcgaa ttactggatg gagtcgtaac aagcgatgaa aagctttaag
     8161 ttcaaacttc gttttgcttt ttccaatata tggaaaaata tcgggaattc gctgtctttc
     8221 gtgctttctt tcgctttggt cgctttgttt gctttgttgc tatcgaatgt gttttcggta
     8281 tcaagacaag cattgtacta ccaatccata gaaaaatacg gagatattga tgttgttgtt
     8341 acttacgacg aggatgcgtc ttctagaatt gtaaatcggc gttctatgac cgaagaatat
     8401 gccgtttatg ttgataaggc atatagcttt ttcaatcttt cggtagtggc agaaacggct
     8461 tccgacacct tctatgtcga tgttatgtca gcgaatacat ccgaatttga attattggtc
     8521 gacacggaac tggggtatgt gggagatacc gatgcggtaa ttaccgaaac catggccaaa
     8581 aagtatgatt tacaggtcgg aaacgacctg acaatgtatc tgttgggaca gacatatacc
     8641 tatgaaataa ttcgaatcat acctgacgaa gggctatttt gcgggaattc cgtatttgtg
     8701 gacaaaacca aaattcttga tgagtttctt ggtcttcccc aactagacaa tttgggtaat
     8761 acgctctata tttgccttga aaatgatatc gaatgtgacg attttattgc catgatcgaa
     8821 gcgaatccgc agtattccga ctatcttatt tatgcttccg tcgatcaaaa tctgataaat
     8881 accagagcga cgcgaatgtc ttccatcctg ttcgggatcg gtgtgcttct ggcggtggct
     8941 ctcgtcatgg tcatgcactc gctttttccg ttgatcgccg ccaaactgag aagccaatac
     9001 ggaacaatag tctctttggg cggcagtggt aaatttatat tgccggtatg gcttgtcgaa
     9061 ttagcggtat attttttgat cggtggccta atagggctgt tttccagctt tttgatattt
     9121 caatacgttg ctcatacata tggaattgaa cacatgataa agcttgattt cttaatgagt
     9181 ttcatagctt ttctggtttt aggcgtattg atgctggctg aagggtatgt caattaccgg
     9241 aagatcgtaa gaactgatcc gataagtctt agtttcaata agcgatatga aaaacagcat
     9301 ttgtatggac tattgattcc gatcacgttt ctggttgtga tcctcgtaat ggtattcaaa
     9361 cctttcacct cggcctggaa cagtttggca atcgttgtct tttctctgat gtttgcgttt
     9421 ctgtcgattt ccgcaactgt aagcaacata tcccgcagta tcaaaaagca tagaccaaaa
     9481 aacctgttcg gttattttaa caacacatac ttgggtgata atatccatat tcaccgttca
     9541 ctcaaaacct tgcttatatc gattttgatc atcgtgatgt cattgtctgt gcgggaattt
     9601 atctttgatg aaaccgccaa agcgaatgag gctttcctgt ttgattatgc tcttgtcaat
     9661 atttttgatt atagtgacga tctgaagcag gaaatattgg atgactatcc tgtcgaaatg
     9721 gctgatgaag cattgttcta tcaaaacgtg tacatcaatt tttctgaaga agaaaaccaa
     9781 gaaacgaatg tcaaaattag ttacttcgtt tcgatcgatc cggacatgtt taattcttat
     9841 ttccggttcg ggttcgaagg aataaataca ttatcttcgc ttccaaacga cagggtatat
     9901 gtcgtcattc ctaaaaacta ttcccagact tacaatctgt cctccggcga taccgtggaa
     9961 cttgacatcg gtccttcaca tgatcccgtc acagcggtga tagccggttt ccttgatact
    10021 aactatgaag gaatagttta ttccaacttg atgatggtgg ccggacacga gacagattac
    10081 aatgtcaaca gcatgcttat cagagccgaa aacgcggaag atattttccg ggaactatct
    10141 atcgattacg gcgaagagat gtattatgtg ctggatatgg acgaagtgat cggagaagtg
    10201 gaatcggaag ttacgacggc cgggaatctg tttcttgtgt tgacggcggt attggtcgcg
    10261 tgtttcatga tcatcatcgt aaacgatacc ttgcttgtgt attattcttt agaaaaggat
    10321 ttggcaagat tgaaagcgat cggagttgga aaaaaggaca tcatgttctc gcttcttacg
    10381 gaagccgcgc ttaccatggc gatcgtttgc gtctttgtac ttttggaaac aatggtactt
    10441 tcagcatatt tgccgaaagt gatgttgttc ttccattatt ataaagaagt cgttccgagc
    10501 ggtttcactc tcatgctggg gttggttctc ggatttgcat cttatgtttt cggatacttt
    10561 ttgtactatc gtaaaataat ccacaccgat ataatgacgg aaatgcggat cgaataagga
    10621 ggaatatatg aaaccgaaaa aattacttgt atgtttaggc gtgctgtcag cgatattttt
    10681 cctagctggc tgtggcaaag acccggtgga aatcgattat gacgaatttg tggcgacgcc
    10741 aactaatctt tccattagtc gtgcggataa tgaactgacg tggtcgccgg tagaaaacgc
    10801 taccgggtat gttgtctata tcgacggcga agcagaatcc accgaaactg ttacgtcatt
    10861 cgatttcact tcttatatcg gcgatgatgt aatcgtattc cggatcagtg ccttggctcc
    10921 gatcggtatg aaagattccg gtcaaagcgt atcaattgct tacaatccga atccggcaga
    10981 ggaaagagcg gcaatcattt ccgttctttc gaataacggt tatggcgact ggggaacaga
    11041 cgacttcata gacgcattga tcacaaaagg tgtgacagcc gaagatctgt tggctttcat
    11101 caacgcgtac aaacaatacg aggatgatgt ggagagcgca tccggcgatc cggtagggat
    11161 aatggatgct ttcgaagaat tcatgtcaca tgtggagaac atcgaagctt tcgtcagcgc
    11221 cctgatgtcg acgaaactaa tgattatcat acaagacgag atcgatgcaa gccaggaaca
    11281 acttgatttt tacatgtcct tatccgaaga aatcagagat cttttttatg ccgaagagat
    11341 tgaaaatctg gagaacaaca tcattgcgat ggaaaacgcc atgttggcta ttcaggactc
    11401 gttcgattat actgttctca gtgtcaccgg cagcatagaa tacctgatga ctgtttttac
    11461 ggatatggat acagtattga catctgtcac tgatatttat caagaaggga tttccggttt
    11521 cgaggatttc aatccggacg aattcatagt gataaaggat gaattggtcg gcgtactcag
    11581 ggacaatgaa ccgagtatgt ctgattgcgt gcttttgttc gaaaccatta acgccatcgg
    11641 cgaagtgttt tccggaatca atctgcaatc agatgatttc gcttcgataa ccgttaccca
    11701 atatgctgca aagatggtga tcggctatca aatcgccatt gaatacatcg actcttatga
    11761 tttggacttt tttaccactt tcaaggatct ggggctgaca tatgccgaag accaaccggc
    11821 aatgctcgaa gcggaaatag agattctcta tttgaaacat gtcaaagcgt tcaaggatga
    11881 caaccagaat ctgttcgacc agtatgacga agttttcaca ccggaagaaa aacagagtat
    11941 tttcggtttc tatgtttcat atttgatgga agcgagcaat attttacaag ctttttccgg
    12001 ttataccgaa tatgaaaacg aaatttccag tttcctggaa aatttacaga atgtcggata
    12061 cgaaaccatg gataccataa gcgacatgag cgagcaatgt tatgataaga tgatgaatta
    12121 ctttgtgaag acagacggag aaatactacg cttgatggca attgcagacc aattttacgt
    12181 aacatatcct gatcatgtat atgtttttgg caattatgcg ttagatatca cttacaaatc
    12241 cgcagacgaa ttgtatatgg cgattttgga aaatcagatc aaaattatcg atcacatact
    12301 gggttatctc gatgccatga ccggagagat atctgacgat gatatacctg cgctggttga
    12361 tctgattcaa acgacgattc catatgattc catcgccgca atattcgaca tggatgaggg
    12421 tgcgatgatc aagtttgaaa cggatatcga cacattgatt accgaacaat tgccgaatgt
    12481 ggtggagtta cttgacaatc ttggcggata tgaaaaattc tacaatgtga tcggacggct
    12541 tgccaaccgg atatcaggat gggatgatta tttcgccaac gacaccgagg actacgtgct
    12601 gattatctat cttttggatt attacggatc tttcatgaat tccggaaacc aggatttggt
    12661 gaacgaaata aagacaggca tattcaagac aatgaaagaa cctgaaatca tcgatatgct
    12721 ttcatcgtat gtctacggat tggacgaagg agacatcaat gaattggaaa acaacttcga
    12781 tctggcgttt gccttttccc tgagcgaggc tgatgttatt tccagacttg acaaaaacaa
    12841 tctgacagta gcagataagg caaggataga cagatatttc gataccataa aggattattt
    12901 catatcttag cgacgaacaa aataaaaagc cgtacgggga gcacccgcac ggctcttttt
    12961 ttttaattag atatcgaccc cgagcaacag tttaaccaaa attccgattc cgaatgatat
    13021 tgcggaaacg ccgatgctga tgattgccat ttgtccgaac ctttttccga acggaagatc
    13081 cttggccacc gatacataat agttgaaaaa gaaaatcacg aatacgacca ccccgagcat
    13141 gacaccgagt gcgatgtaag gatcggataa aagaagatag ggaagaatga gcaaggtgac
    13201 ggtgatgaca tatgccaccc cggtataaag agacgatttg agagctttgg gattgttgtc
    13261 ggcccgttcg ctcagatatt cgctggccgc catcgacaaa ctggcggcga tgccggttat
    13321 caagccggat aggctgatca gtctggtgtt agccaaggcg aatgtcaatc ccgccagagt
    13381 tccggatatt tctaccaaag catcattcag cccgagaacc atggaaccga cataactgag
    13441 cctttcttcg tcgagcatgc cgatcagttc tttctcgtga tcttcctcat cctgagatat
    13501 agctatggct tcctggacgt atttggcgat ttcgtggtaa tcggtcttcg ccttgtcctc
    13561 gcgtttctcc attgttttga gagcgaaagt gtatccgaag atcgctgtca atatccggta
    13621 gtaaagtatc aggccctttt tcggcttggc cgccctacct aaatattggg accagatgtc
    13681 ggaatggtgc ttttcctggg aagctatttt taaaagaatt ttgcggttgc cggcgtcttt
    13741 tgtccgtttg gcaatatgtg tgtatacgta atattcggtt atctctcccc gttgcaaaga
    13801 tttgatttgc gacagtattt cttccggtat gtcttgcatg atatcaccct tgctttcgat
    13861 tggtaataat tttaccacaa atccagacat aaatccatta tgaggcggtt tattataact
    13921 gattttccgt catcgtcgat gtctgagagt tttacaaaaa cataatgaaa aattttttta
    13981 atgtgatata atcaaaaata cagatgaaaa aatacattgt gaaagcaaac gagattgggg
    14041 gcgcatttta ttttcgcccg gtactacaaa ctcgacgcca tatgaaacga taactccaag
    14101 gaggatacag aattcatgga acagtgggac atcatcattt atttcatttt gatcggcgtg
    14161 cttttgtttg ttggaaagat tctcaagacc aaattacctt tcctgaatcg cattgttttg
    14221 ccgacggctt tactcggcgg tctcatcggc ctgttgttat caagcgtcgt gattcccgga
    14281 tcatatataa ttgatacgga agtcatgggg ccaatcgttt atcatgcttt ggctttgggc
    14341 ttcatcgctt tgacgcttaa acaggaaaaa agcaaaaaca aacgcaaagt atggtcaacc
    14401 ggaatggtca tcaccagcac ctatgctttg tagggtttca tcgggattct cctggtattg
    14461 cttttcttct ccgataaatt cgtcggagcg ggaatgttat tgcctctcgg attcgggcaa
    14521 ggccccggtc tggcatacag ttttggaaac atgtggagcg agctgcttgg caaccaggga
    14581 gcatctcttg gtatgtccta tgcgttttta ggctttgtgt tcggcggcac ggtcggggtt
    14641 ctttgcatca atatcctttc tcgtaaaaag ggcgtgcaaa agccaacgca atacgaagat
    14701 gtttcgcttt acaaaacgac tatcgaagtc gataccatca aagaagtcag cgtacttgac
    14761 ggccttaccg tccaggtggt catcatatcg gtgatatacg gcttggtgtg gggtacattg
    14821 tatcttgtca aggcgccttt aatgtcgcta aataccgaaa ccagcaatat cggaagcact
    14881 attttcggac tgatggaggg atttaacttc atcatcggta tcggctatgc cttgctttac
    14941 aaaataattc ttaacaagct tcggaagaaa aacaaaactt tgaacttcat ggtcaacgat
    15001 tatgtggttt cgaacattgc ttcgctttgt ttcaactaca tgattgccgg cgctgtgctg
    15061 gcgatcaccg cggatttttt gaaagactat ggaatcttac tcgctgtcgt ttcggcagtc
    15121 ggaggcgttg ccactctctt ttatcttaga ttcatcactt acagggttta tccaaattac
    15181 aaggcggaat attttgtcgg cttgttcgga atgctatgcg gcgtcgcatc gaccggtatc
    15241 gctttgctta aggggcttga ccgcgatctc gaatctccgg tagccgaaga aatggttctt
    15301 ggttccggaa ccgccatcac catggcgctc ccgctgttcg cggtactgat attgccctcg
    15361 cttgggtatg ataaatttgg gaacccgaca gccaatttgt ccttgtataa taatattgcc
    15421 ctatttggat gtcttgcgtt tgttttggta atggtgggaa ttctcttgtt ccgttcaaga
    15481 aaaaaagtta ctcagtaaat caatttgatg atgccgtgat ttttacggca tctttttgat
    15541 tttatatggt gtaaacgaac ggaaaaatgt ataatataca tgaacagaag gaggttggat
    15601 attcatgcaa tatcgcatta ctgaaaaagg aaatttgctc aatgaaaaag gaagactggc
    15661 gacatccggg tacgctacca gccttttgct acattacgac cggaatgaaa ttgccgcttc
    15721 accctggcgg atcaaagaat gggattacta tgccgttctt acagccaaat acggagtcgc
    15781 attcgttgtt tccgatctgg gatataccgc gatggtttcc gccgttttct ttgatttcgt
    15841 caaaaagacc caaaaatcca tgactaaact tttctggttc acattcggga aaatgggact
    15901 gccttcatca tcggaaaaag gcaatgtaag ttgtcaccaa caaggatatg atttcgattt
    15961 catccgcaat caagatgaaa gactgatcaa ggtgacaatc aaggatttct atccgggaaa
    16021 ggatttgcga gcagatctca ccctgaaaga catgaaggat gaaaccatgg tcatcgccac
    16081 tccttggcag gaaaaaccaa aagctttcta ttataatcaa aagatcaact gtatcccagc
    16141 gtacggcagc gttttgatcg gcgaagagtc ctatgcattc aaagaaggag aggcatacgg
    16201 agtgctcgac tggggaagag gcgtctggac atacaagaac acatggtact ggagcaacct
    16261 ttccggaata gttgatggtc atcgggtcgg tttcaacatc gggtatggtt tcggagacac
    16321 atccaaggct acggaaaaca tgctgttcta tgacggcgtc agccataaac ttgaccaggt
    16381 cacttttgaa attcaggaac cggatttcct gaaaccatgg aaattcaccg acaacgaagg
    16441 aagattgtcc ctgacaatgg agccgcttgt cgacaggcag gaccacacca acatgatcat
    16501 catcaagaac atgggacacc aagtctttgg aaaattcacc ggatttatcg tccttgacga
    16561 cggaaaaaga atcgagctca aggatatgat aggatttgcg gaaaaaataa cgaatcacta
    16621 ttgagaggta aaaaatgaca aacaaagaat tgtatgatct tctaacgggc gacgggttct
    16681 gcacgatcga actggatggt gacgacaaca gaaaatacga aatggaacaa atcggcttgt
    16741 tgccgatgca cggtgtgaca tatgccatca tgaacatctt aaaagtcgac gatacgccgg
    16801 tggataaaaa cgatgcaggc gtggttttgt tcgaactgga tttcgatgac gaaacggaag
    16861 agtattttgt cgccaccgtg gaagaagacg atgtgtttga agaagtaatg acggaattcg
    16921 agaaaattcc gccgactgat taataaagca aaacgatatc ttttttattt ggaatatgat
    16981 atcatattgt caagaaaagc aaaaaaattg agaggagtgc tgtaaatgaa tatctattac
    17041 tgcaagcact gcaaacgaat ttgttatact tacccggtcg atagcacctt ggtttgttgc
    17101 ggagacgaga tgacgcttct ccagccaaag acaagcgatg tcggaaatga aaaacacctg
    17161 ccggtggtgg aaagaattcc aaatgacatg atcaaggtca cgatcggaag cgtactgcat
    17221 ccaatgttgc cggagcattt catccaatgg atcttcatcg cccaggatga ctcttaccaa
    17281 atcaaaacat tcaaacccgg agacacgccg gtggcaacgt tccacatcaa tccggaccag
    17341 gacatcaagg tttatgagtt ttgcaatctc cacggtctgt ggatgacgga agtaaaaaaa
    17401 taacaatcaa tagcaggaaa tgccggtttg taaaaaccgg ctttttcttt acccagttgt
    17461 aaaaaaatat tccttagtgt aaaattgcta cataaggagt tgagagtgat ggatatcctg
    17521 gaattgctga aatacttgct gcttggattg atccagggat tggccgaagt tttgccggtt
    17581 tcttcaaccg ggcaggtcga actggtcaga agccttatcg gtgtcgatgt ggaaaaagaa
    17641 ttgctttttc tcattttgtt aaacaccggg tctttcatcg tgcttctttt tgctttcaga
    17701 agacagatag gcgttctgat ccgcgatttt tttcgttata ttttctccgg taaatcccat
    17761 cccgagagca agccggggtt tgtatactgc atcaagctta tcttagccac gctcccggcc
    17821 gctgtcgtcg ggttgttttt tcgtcacaaa atcgatggtt ttttattgaa ttatggaact
    17881 ttgttttccg gaatcgggct attggtaacc ggaacagttt tgctttatgt cggatttggt
    17941 gattaccgtc accaaaacgg gaaaatcggt tggctcgact cggttttgat cggtgccgga
    18001 caggctttgg cgttgatacc gggaatctcc cgttccggga tgaccacttc aattgcccta
    18061 aaaaggggag tagacatcga ttctgctctt gatttttctt ttcttatgta tcttcccgta
    18121 tcgtttggcg gacttgtgct ccttttgtta gaagcgtccg gaagcggatt ctcggttcct
    18181 tccccatctc attatgttta ttatgccgtt gcttttttgg gagcggctgg attcacttat
    18241 cttgctttta ggattattta caatgttttt cgttccggaa agctgaaata tttcggatat
    18301 ttctgtcttg cgaccggaat tctttctttg attgtttatg cattttagct gacgggaaaa
    18361 cgagcgaaaa taataggcaa aggcggttta acgaatgtct atagtggaac tcattaaata
    18421 catattctta ggacttatcc agggatttac ggaggtattg ccgatatctt caaccgggca
    18481 cgtcgcctta tttcagatca tcctaaacat cgataccgat tcgggtctcg tttttatggc
    18541 aatcgtcaat ttcggttcct tgttggcggt ggtctggcat ttccgattat atatcggtcg
    18601 cttgatcaaa aacttttttg tattcatgtt cgataaggaa agcagaaacg aaactcgaga
    18661 cgactttctg tatgccatta aaatcatcat tggcgttatt ccggccgctt ttgccggttt
    18721 gttcctcaca ggttatgtca acgaaatata caagcgatat tctttattgc ttgttggtat
    18781 cggattgctt gtcacatcca catttctttt cattgtcagg aaaggcccgg aacgccacgt
    18841 caagcaaaca ttgaccttca aggatgcgac gatcattgga ttgcttcagc cgttttcaat
    18901 cgttcccggt ttgtccagaa gcggcataac cgtctcgggc ggattgttga gaaaggcatc
    18961 gatggatacg tcgctttctt tttctttcat gctctatatt cctttgtcga tcgggtttgc
    19021 ttgtcagtcg gtgtattaca tgatagtgca accgaccgga cacaatttag gctttgatcc
    19081 atcggattta tatcaatata tatattatgg tgccgctttt ctcgccagct tggccgcaac
    19141 caggatcgga ctcaaataca ttttcaaatg gttccgaaac ggcaaattgg tgatttttgc
    19201 atactatacc cttttccttg gcatgattgc cctggtggcg ggagcggtga tcctttaagg
    19261 acaaatttcc gacccgattg aattatcatc gtcgatgatg tagaatttaa ttatgcttga
    19321 tgaaatgacc gaactcattt tcgaatagga gagaatctta tggaagaatt tgaaaagcag
    19381 gaaacaagca cggaagaagg aatgactttt accgagttgt tacgtatcgt atgggacaat
    19441 tttcttctga ttgttttaat cacagtatgg atcacggcct tgggattcgt atacaccttt
    19501 gcggttgtat cgcctaagta tacagctgaa accgccgcga tggtccaagt ggatactgat
    19561 tcggggactt cggaacaatc ggcaatcagt attgctaatt acctgatgac gacatataag
    19621 gaattcattg tcagcagact ggttttggaa agtgtgataa acgatgtcga cggcatcagc
    19681 gacatgaccg tggagcaatt ggcaaacagc gtcaccgtct ccagtacgag cggggcttac
    19741 ataatttata tcaatgtcga caacacatct ccggaactgg cccaggcaat cgccaatcag
    19801 atcgtggaca acgccattgc gatagccgat gacgaagcca acgggttcac ttttcttcag
    19861 gacaagctga aacttgttta ttcagccgcg cttccggaag cacccagttc gccgaacaaa
    19921 ccgttgaata tcatcattag ttttattctt ggcggcgtca tcgccttgat ggttgtgttc
    19981 ttcaaagaga ttttcaataa caaattccag accaaggaag aattggaaaa agtcctcaat
    20041 ctgcgggtaa tcgccacggt tcccggcacg atcaaagaga gaaagttggt ggactgaatg
    20101 gaatatttcg aatatcataa cgtagttatc gaatctccga attcacggga ggcggaagcc
    20161 taccggaaac tggaactcaa tattgccatg atgtctttgg atgaaaagat ccaagtcatc
    20221 caaagcacgt ctgccattcc cgaggacggc aaaacgacga cagcgatcaa tctggctgcc
    20281 gtatatgccg aaaaagggga aaaggttatc atcgttgatt ttgacctgag acgtccgaaa
    20341 attcacaggg cattcaaaaa gccgaatgaa aaagggttct atgattatgt gaatggcgac
    20401 atcgattttc acgatatcat tgttcacgac gaatccggga ttgatctgat tctgacgggg
    20461 aaacatcttt ctttccccca cattgtctta gagtcccata aagcttccga cctgattgcc
    20521 aaattgcgta aggaatatga ctacatcata gtcgacactc ctccggtatt gtccgtgacg
    20581 gattccctta ttgtcagtaa atatgcggat ggagtccttt atgtcgtcgc ttacaataaa
    20641 acgaaaaaag atgacgccaa ggccgggttg ttgcaactgc aaaaaaatca tgccagggtg
    20701 ctcggaagcg tgtttgccaa catcgatatc aaaaagttca aaggctatca cggatacgat
    20761 tattattcgc atcaggataa aacgtcctga aagccggtga taccgtgatt gatatccatt
    20821 cccacgtcct gccttttgtg gacgatggat cggacaacat ggagatgtcc cttgccatga
    20881 taagggaagc ttcagaggct ggggtcaccg atttgttcct gacgccgcat tatatgcgcc
    20941 tgagggacta tctttccaat gcgtctgaaa acagagaaat tttttccgaa ttgacgaata
    21001 aagtcaaaca ggaagggatt tctatcaaac tttatcttgg aaacgagatc tattattcgc
    21061 ttgatatcct ggacgccttg agaacgggga ccatcattcc tctgggcaac tcgaacaaag
    21121 ttcttgtgga attttcgctg tttgaagaag acgaggaaat aaccgaggcg atccacaata
    21181 tcaaatcgct cggatatgtg ccgattattg cgcatccgga aagatatccg tatatttcca
    21241 aactctcaga ttatgaaata atcaagcaaa tgggagcttt gattcaacta aatgcttcct
    21301 cgatcatcgg gcaacgtgac aaactggccc acaagaaagc agtggccata atcaaagccg
    21361 gactcgccga tttcatcgct tctgaccaac atgtcccggg aaaaaatcac ctggaagaag
    21421 caaggaatat cataaacagg aaattcggtt tgccagtggc gaaggcattg tttgaaaaca
    21481 ccgttgtttt agaatgacac ttcttttgaa gtgtttttca tttgctcaca aatacatcat
    21541 attgtttagg tataatcaaa ttgaaaaatt caaaaatcgg tacatttcac ttctccccct
    21601 atgaatgatg gtatcttatt tttgaatttt ccaattggta cgggtcaaac ccgtgccagc
    21661 cgccaaattc aggtcttcgg accttgcatt tataaatcta aggcttgtca aagtctttta
    21721 ttttctaaag gtgtgtgatg tttttatgaa aagtttttat gttttagttg cagacgacga
    21781 attcagaatc agaaagttat tggctgattt tttggttagg gaaggctata aggttttgga
    21841 agcatccgat ggcgaggaag ccatcgactt gatttttgaa tccaaaatca agattgatct
    21901 ggtcattttg gatgttatga tgccaaaata cgacggatgg tttgtcttgg aaaggattcg
    21961 agaattctca acggtgccgg taatcatgct tacagcgaga agcgatgaaa gcgaccagtt
    22021 gaaaggattt aaattgggag ccgacgatta tgtgaccaaa cctttttcgc ctagcatcct
    22081 gatggcgcgg ataaacaaac tgatcaaacg cgacaatgtc gaaacaaccg aactagtttt
    22141 tggaaagatc aggatgtctc tagcttcgcg ggaagttttc gtcgatgaaa acaaagtgga
    22201 actgacgccg aaagaattcg aacttctcaa atattttatc gacaacaaag gcatcgcttt
    22261 gtcgcgtgac aagattctga atgccgtatg gaactatgat tactttggcg atttgagaac
    22321 agtcgatacc cacatcaagc agctccgtag caaactgggc gattacgcgc actatatttc
    22381 caccgtgaga agcatcgggt accggctgga tatcgaaaat gagaacgtca attaagagcc
    22441 gactgtttct gatcacctat ggtatcatcc tgtgtttcat actcggtttg atcgtactca
    22501 acaacactta tttggaaaaa tattacatga actatcgtga aaacacgctt ttgaatgctt
    22561 tcacggaggt aaaaacaata gatttgagcg aggacaccta cgattccttg ctctttgacg
    22621 tggaagaaaa ctacaacata agcattcaag ttctgaaaga gaggggcatc caaccgaata
    22681 tcgataatct tccctcgcca tatttaagac tatatggcaa taccatgtcg atcagtcccg
    22741 gaatcatcag tcagatcatg ttgcaattaa atttgctaga cgacggatat tccagcagca
    22801 gttcggtcga tgtcggagat ggttacgtgg cgttcgtcca aaacatcatt cccacatcat
    22861 cgaccaacgg cgatgatgtt caattgctta gtttgtgcgc tgcgactgat tctggcgacg
    22921 ggttgctgat gtattacatt ctgactgtca cggttcaatc gatccgcgat agcatcagcg
    22981 ttttcaatac tttcacgatc atcatcggat tcattttcat gatcatttcc ggaatcttta
    23041 cctatttgat cagttatcgc ttcaccaacc cgattctgga gataaatcaa gtagctcgcc
    23101 agatttccaa tctggatttt ggtagcaagg tggaggtgag ctccaatgat gagattggcg
    23161 accttggcaa cagcatcaat ctgatgagca cgcaacttga gaatgccatc agcaagctca
    23221 aggcggccaa tgaaaaacta gaaaacgata tccaactaaa agagaagatc gattcgatgc
    23281 gcaaagaatt tattgccagc gcatcccatg aattgaaaac gccgatatcg ctgattctag
    23341 gatatgcgga agctttgaaa cttcccggta tcgacaaaga taccagcgac gattatctga
    23401 atatcatcat ggacgaatcg aacaagatga acaaactggt catggaaatg ttgaagatat
    23461 cacaactaga aagcggtttt tccgaattca ccgtcagcga tttttccgtg aaggatatga
    23521 cggatgaaac cgccaggttg ttctcgatca ttttcgaaga aagtaaaata aatgtgacaa
    23581 tagatgtctt agatcgggaa gtgtcgtccg attacgacca attgcagacg gtattgtcca
    23641 actttatttc caacgcaatc catcacgtgg ataaaaacaa aaaaatcagg atttacagcg
    23701 aagaaacaaa tgaaggcaat ttgcggcttg tcgtattcaa caccggcttg gcgatcgcag
    23761 aaagcgatca ggaccgggtt tgggaaagtt tctacaaagt agacaaggcg agaacccgca
    23821 gctacggcgg tcaagggttg gggctgagta tcgtcagaaa caccttggac aacatcggtt
    23881 acggctatgg tgtcaaaaat gtcgaaggcg gcgtggagtt ctatttggaa attccggcta
    23941 aagatataaa aagtaacaat caatagcgaa gaagatattt gcttctgaaa cgtaatgaca
    24001 gcgttttttc ctttgctttg tattgaaatg ctcaaatacg tatgttagaa ttgtcaatat
    24061 tcgaagtcac tctaggggga ggaaacttta aagtgcgacg cataaagaat gagtcaatgg
    24121 gcgattacat gagaaaaatt cggatcatcc agtacatatt tgttgatgcg gtttgcatag
    24181 tattcaccta ttcgctgact tttcttgcgc tcaaatttga cggagcttcc aactttgaca
    24241 tcgagaccgc tttcaaggtc ctgcctttgg ttttggtatt caaactggtc gctttttatt
    24301 tgatgaaagt atacagattg gtcatggaca acgctggcat cgaagaagta ttcagaatct
    24361 tcattgcggt gataacaacc aatatcgtca ttacgctcgc ttcgcttttc attcccaatt
    24421 ataatttcat accggaagta agcatgatat ttaccacggc tttcgaaggc attattcttt
    24481 ctgtgcccag ggttataaag aggcttacca acttggtatc aagaaaacat ccggtcggtg
    24541 gaaaacgcac tttgctcgtg ggagcagggg ctggcgggaa attggtattg aatgaaatcc
    24601 gtaacaatcc cgaactcaac aacgttccga ttgcttttgt ggatgacgac cctttgaaaa
    24661 taggtaaaat aatgtctgga tgtccgattc tgggcccgat cgacaaaata ccgaaattta
    24721 ttgacatata ccagatcgag gaagtcataa tatgcattgc caatatcgat ccgaagtggt
    24781 ttcaggaaat cattcagaca gccgccgaaa gaccggtgag aatcaaaaga ataccgaaat
    24841 tcaaggaatt gaaaaaagat acgccgaaaa agtttctgga aatcaatgtc gaagaccttt
    24901 taagtcgtga tgttgtggag cttgacagca gcgggatcag cgaatttatt tccggaaaaa
    24961 gagtcatagt cactggggcc ggaggatcta tcggttcgga actggtcagg caaatcgtag
    25021 acatgagacc gtccgaattg ttgatgttgg atatttacga gaacggcgtt tacgacatcc
    25081 agatggaact aaaccataaa tacgccacat cggacgaaga accagttcaa agaaaagtat
    25141 tgattggttc agtatacaat tacaatcgga tgaatgccat attttcagct ttcaaaccac
    25201 aactcatatt tcatgcggcg gcatataagc atgtgccgct gatggaagat tctcccatcg
    25261 aagcggtcag aaccaatgtc atcggaacat acaatatttg caaactggca tcgctttaca
    25321 atgtggaaaa agtggttttg gtttctagtg acaaagccgt cagaccgacc aatgtcatgg
    25381 gagcgaccaa acgatttgcg gaaatgatca tgcagtattt caatagtgtg tcctctacca
    25441 aatactcggc agtgcgtttt ggcaatgtcc taggcagcaa tggatccgtc gtaccgctat
    25501 tcaagaagca aatcgaaaac ggaggtccgc ttactgtcac tgatgaaagg attattcgtt
    25561 acttcatgac aattccggaa gccgtcgggc tgattctcca gtctggaaca ttcgccaagg
    25621 gtggcgaaat cttcattctg gatatgggag atcctgtcaa gataatcgac ctagccgaga
    25681 aaatgatcat gctttctgga agaagaccgt atattgacat tgatatcagc attaccgggt
    25741 tgcgtccggg agaaaagatg tacgaagaac ttttggttga caaaacaaaa gacataacaa
    25801 aaacagccaa caaaaagatc ttcatcgaca ggggctcgga aataatgccg atcgaggaag
    25861 aaatccgcga tatcacagga aagttcgaaa ccctaaacag cgttgaaatc aaggaaatga
    25921 tcaaaagcat ggtttcaact tacaccccaa aataaaaggt acgggcatcc gtgccttttt
    25981 tgattccata tccctttata tattatggta ttcgtggtag aatatagatg aaaaaaacca
    26041 agaaacgcga ggcgacgacg ccatgttttc attagaaaat ggcagcaagg taaaactgga
    26101 aatccgcaag atgggaatca acggcgaggg catcggttat ttcaaccggc ttgccatttt
    26161 tgtgcccaga gcgattacca aggaaatcgt caattgcgag attgtcgaac tgtatgacaa
    26221 atatgccgtc gccaaactgg aagaagtcgt caagccgtcg aatcggcgtg tcgagccacc
    26281 gtgcaaattc tatgaacaat gtggcggttg ccagttgcag catgtcgaat acaaagaaca
    26341 gctcaaagcg aaattgagcc ttctgaaaca atcgctttcc cgatatacga gtctggacat
    26401 cgcttcgctt aaaatccgaa aggcactcgg tttggcgaac aattaccatt atcgcaacaa
    26461 atcccagcgt ccattcaaaa acaccaattt cgggctggct ctcggactgt ttcgccaagg
    26521 cacaaaccag ttcgtttatg tcgacacatg catgacggaa gacgaacatg tgaatcagat
    26581 caaccaggaa gctttaacgg tattgagaaa gtatcacgtc atggcttccg accaaatgaa
    26641 taaggaaggt atccttctaa atctcgttac cagatatctc gcttcaacgg aaactgccag
    26701 tgtgacgttt attgtgaccg atttcgatcc tgtgttgaaa gaagtcgcaa aagatctgat
    26761 ggcaaaaaat ccttccgttg tttccgtgac atatagcgtg aacaaaaaaa ataaccccat
    26821 gatgttcgga ccggcggtgg aacttttggc cggtaagccc tatattactg atttattttc
    26881 caacatgaaa atacgtatct cccccgatgc ttttcatcaa ttgaacacgg cgcagatgaa
    26941 ggttttgtac gatgagatcg tcaaagcggc ggaattagag ggcaaagaga cggtgattga
    27001 cgcttattcc ggtattggaa tgacatcttt gatgatggcc agaaaagcga aaagggtcat
    27061 tgggattgat tattctcccg cctcgatcgc agatgcgacc aaaaacgcca cagcgaatgg
    27121 aatcaaaaat gtttctttta tctgtgacca tgtcgaaggc gcccttccca aactgatcgc
    27181 aaatggagtg aagccggata ttcttgtatt agatcccccg cgggccgggc ttcccgaaga
    27241 gatcgtgaaa ctgattgtca aaactaaaat caagaaaata atctatgtgt catgcaatcc
    27301 gtcgactctg gcaaagaaca ttgccggctt agaaagcaag tatgaggttt gctacatcca
    27361 accgcttgat atgtttccgc atacggcaaa tgtcgagagc gtcacattgc tcaagttgaa
    27421 agagagcgga ggtgcgaacc gttgagaatc agagaatacg tcatgccgaa gtcgctcatt
    27481 tatacgggcg aacatccgga agttaaaaca aagatcgtcc attaccaata tgatgaaaat
    27541 aaagttgaaa taacggataa tttcgtttcc cttcccggta aaaagcatta tattcaggtc
    27601 ataggcctca aggaaatcga caaggtcaaa acgataaggg aattttactc gatcaatccg
    27661 ctaattctcg aagacgtttt caacgtcaag caaagaacca aggtcgagat gcacgacgat
    27721 ttcctgtttt cctcattgca tctcgaatat caggacggcg aagagatcaa gcatgattat
    27781 ctgagtattt tcatgaacaa ggatgcgttg atcactttcc atgaaacgga gccggcctat
    27841 cttgcggctt tacctggcct ttttcaaaaa aacgaggaat tgcgtaaacg cggtatcgat
    27901 tatctttatt accaaatcct cgatatcgta accgacaatc atctggatat ttatgacacg
    27961 cttgagaaac gggcgaatga tttcgaagag gaaatcctgg aaaacgagaa aatcgaccag
    28021 gaaagtttct atttgaccag aaagcaattt ttgaagttga aagaagcggt atcgccactt
    28081 atgagcgaac tgcgtgtggc gatgggatct gcgacagctg gcttgctttt ctctaaagac
    28141 accgagccat attttcagga tttgcgggac cacttgcaaa ggcttgacga tcaattgaat
    28201 actgcccgtg aggaaatccg ccaccttctc gatctgatga tgaataacca gtcatacaaa
    28261 atgaatcaga tcatgacgac tctcacgttg ttttcagcaa ttttcatccc cctgactttc
    28321 attacgggaa tcttcggaat gaatttcaca tatttcggca tccttgatta caaatatgcc
    28381 ataccggcat tcacagccat ctgtgttttg ctggcagtcg gcatgcttat cttcttcaaa
    28441 aggaaaaaat ggttttaaga taagaaggga aaggacaaac aagaaatgga ctcagatctg
    28501 caaattgccc aaaaagcaaa aatggagacg atcacaaaga tcgccggtaa gctcggattg
    28561 aaagactccg atatcgaact atatggcaac tacaaagcca aaatcaacat ggaagcggtg
    28621 aaatccgacc gctttcaaaa gaacggcaag gtgatccttg tcaccgctat taacccgaca
    28681 ccggccgggg aaggcaagtc gaccaccacg atcggactag gcgacgcctt gaaccgactg
    28741 ggaaaaaata ccgtgatctg tctaagggaa ccatcgctcg gaccggtcat gggcgtcaag
    28801 ggaggagccg ccgggggcgg ctacgcccaa gtggtaccga tggaagacat caacctgcat
    28861 ttcaccggag acattcatgc cgtgacggct gccaacaatt tgatcagcgc cgtcatcgac
    28921 aatcatcttt attttggaaa ccagctcaat atcgacccgg aaagaatcgt ttggaaacgg
    28981 gcgatggata tgaacgaccg ggcattaaga aggatccagg tcggtttgtc aagcaagaag
    29041 gaaactccgc gctatgacgg tttcgacatc agcgtggctt ccgagatcat ggcggttctt
    29101 tgcctggcaa gggatatcga tgatctaaag aagcgaatcg ctcgtatcgt cgtggcttat
    29161 gataaaagcg gcaatccggt aacagtaagc gacttaaaag ccgccggtgc cgccacgatg
    29221 ttattgaaag acgctatcaa accaaatctc gtgcaaacct tggagaacaa tcccgtattg
    29281 atccacggcg gtccttttgc gaatatcgcc cacggatgca actcgttgat tgccactgat
    29341 tttgccagaa gagccggcga ttatgtagtg accgaagccg gtttcggtgc cgatctcgga
    29401 atggagaagt tcctcgatat caaatcccgt cttttgggag tgttgccttc ggctattgta
    29461 ctggttgcca gcatccgcgc tcttaagcat cacggaggcg tcgaaaagga caaattagca
    29521 gaagaaaatg tcgaagcttt ggcaaaagga atcgtcaact tggaaaagca tatcgaaaat
    29581 atcggttttt acaagattcc tttcgttgtt gcccttaaca gattcttatc cgacagccaa
    29641 gcggaaatgg attacatgat gaactgggcg aaagaaaaca attgtccgat ggcgctttcc
    29701 gatgtgtttg ccaacggatc tgccggcgga atggaacttg caaaaatggt catcgaaacc
    29761 attgaaaaca agtctagtca gcattccggt ttgttatacg atgtcgactt gtcaatcaaa
    29821 gaaaaaatcg aaagaatcgc caaaaaagta tacggagccg ctaacgtgga atattcggaa
    29881 gcggcggaaa aagcgattga gacttatgat gctttcggat ggaacaagat gcccatctgt
    29941 atggctaaaa cccctttgtc attgtcagat gatccgaaaa aaatcggacg tccagaaaat
    30001 ttcacaatca cgatcaggga tatgaaacca tcgattggcg ccggttttct ggtggctttg
    30061 acgggcgatg tcatgacaat gcccggcttg ccaagaaacg gagccttcga aaacatggat
    30121 gtcatcgatg gcaaaataat cggactcttc taaaacagga ggaagcaaaa tgaaaaaaga
    30181 aatcagcgac accatgaaaa aattgcgcga tctcgggctt aagatcgcca agactgccga
    30241 gaaagggtat caacaagcca aatcaagcgt ggaaagcacc atactcgaag acagtctgcg
    30301 tcgccggttc aacttggaaa atccatataa attccaaatc cgcagagaca attccgtgtc
    30361 cgacctgatc gaaggcatca tgcctcggaa tgccaaaaga tatgatgagg acgacatgtt
    30421 tgtgttctat ggcgacaagg aatcgaacaa ccttacaaaa ggcgatctca tcaaagactt
    30481 gagttccaac gccgattaca gaatcaagga tctgatcgag gtggaagtac ccgtcgaact
    30541 ggacgggaaa acctatcagg tcatcggcac cgccgccctg tgcgatctct tgtaggcatt
    30601 atggcaaatt accaagacga attcatcgat ttataccgga aacatatcag acgcccggga
    30661 tccgaggaac tgttgcaata tcttttgtcg gatcaatccg actttttcac agcgccggca
    30721 tcgaccagat ttcatctgtc ccatgaaggc ggcctcgtcg aacattcgct aaatgtctat
    30781 cattgtctga gagactatct tggcagagaa cgcgtgacgg acgaatacaa atgccaacca
    30841 tcggaagaaa gcgttgcgat cgtcgggctt ttgcatgatc tatgcaaggt taacgtgtat
    30901 cgaaaatcgt tccgtaacgt gaaggatgcg aacggaaaat ggaatcaagt tccgtctttt
    30961 gactatcgtg acgatctgcc atacgggcac ggagagaaat cggtttatat catatccggt
    31021 ttcatgcgtc tcacgcgtga agaggctttc gccatccgtt atcacatggg attctccgcc
    31081 gatatggaaa acaaggctga cgtctcgcgt tcgttcgaac tatttccttt ggcgctttgt
    31141 ctatcgattg cggatatgga agcgactttt ttcctagaat catccgaaga ataatttcaa
    31201 aaccccgtta aaacatgaca tgaggtgaaa catttggcac gcatttataa tctgaccgaa
    31261 ggcagcatca taaagaaact cttttatgtc gcgcttccgg tcttgctttt gtcattgtcg
    31321 caaatggcat acaacttgac cgacattttt tgggtggggc atgtggaact gatcggaata
    31381 gtcgaaagcc aagcgatttc ggctgtcgga accgctgctt atatcacctg gttcgctttc
    31441 gggatcatca tgatcggaag gatcggcacc agtgtgaaag tagcccattc ggtcggagaa
    31501 aacaaaaccg accggttggc cggatatgcg gaaaacggtt tgatattgca ggccatactg
    31561 ggcctgtttt tctcagttgt cgtcgtggtg tttcgcagac agatcatctc gatcttcaac
    31621 atcggcaacg aaaacgtagt cgagtacacc gtcaattatc tggcgattgt cggttcgttt
    31681 ctattcgtcc aattcttgac atacggattc gtcgccatca atgatggcgt gggaaagacc
    31741 atgacgaact ttttgatcat gctgatagga atcgtggtaa acatgatttt ggatccgctc
    31801 tttatcctgg ttttacggat gggtgtttcg ggagcggcac tagccactgt gatcggacaa
    31861 atcatcactt tggtcgcatt tgtttttctt tacgccaaga aaaacaaaat gttccgtttt
    31921 cgtctgtcgg catttcgcaa gaagtatctt ctggaaatcg tcaagatcgg attgcctacc
    31981 gggttgcaaa gcatgctttt cacctcaatc tcgattttta tcgcccggat ggtttatgaa
    32041 tacggtgagt atgtggtggc tgcccagagg gttggcgtgc aaatcgaaca gctgacatgg
    32101 atgatagccg gcgggttcca aaccgccctg acggtgtttg tagcccaaaa cttcggagcc
    32161 cgtttgcatg gacggatccg gaaagggttc ggaatcatgt ccggactgct tttgccttac
    32221 gctgctgcgg ttgctttgtg cctttatttc ttttcggatt ttttgctcgg gatattcata
    32281 agcgatccgg tttcgctttc ttacggagtt gcttatctca aaataatcag tctggcccag
    32341 attttcatga tgatggaggc aatcggcacc ggactttata acggagtcgg gaaaacctat
    32401 attccttcga ttatcggaat tgtcggaaac gtgcttagaa ttcctttggc gatcgaactt
    32461 gtgaaagtta tcgatgaaaa cggaatttgg tggtctttga atatatccga catgttcaag
    32521 ggggcgattc tttatgccgg atcactgatc ctgcttgcgt ttttggaaag aatcaaacta
    32581 aacaaaaaac agaaccggca attggtagaa gaaacatgaa aaagcgatgt tttttgggta
    32641 tcaggtccgg ttttttcggc caaatggccg acaatgtgct aaaatatata gtgacataat
    32701 caaatattca cagaagggat gatcgctcat ggattggtac tggtgggtgc ttatctttct
    32761 ggctctgatc gcaatatatc ttgtcaacat cggttatctt gttgccaaga tgctttttca
    32821 tccgaaaagg atgacattat ccgagaccag aaaaagagaa ttggaaaaaa gtccggagat
    32881 attgaaagcg acaaaagatt ggcgtttttc gatgtatcag atcgaatcgc gccataaata
    32941 tcctctgcaa ttgtattttt tgccggctga aattccgacc aagcgttata ttgtcatcgc
    33001 tcatggttat acgtatacga gccatggcgg ctggaaatat gctaccttca tgagaaatta
    33061 cggctttaat gtcattctct atgacgagag ataccatggc gcttccggcg gtaaaaattg
    33121 taccatggga tactacgaaa aagatgatct gtacgatatc atatctgata cttataaaag
    33181 attcggtaac gatatctatc ttggaacata tggggaatca atgggaggag caatcgttct
    33241 catggaacag tccggcgatg accgggtgaa attcgtggtg gccgattgtc cgtttgccga
    33301 tttgagtctc ttgctaaaat atctgatccg gaataaagtt catttgccca tgtatccggt
    33361 aatatggata gtcgatttct ttttccggtt atttacgaag accagtattt ttcggatatc
    33421 gccgattgaa gaagtccgga aagccaaagt cccgatcctc ttcatgcatg gaggcgaaga
    33481 cgcttttatt ccaccgatcc acagcgaact tctttttgct gcctgtccta ccggcaagga
    33541 tcttttcatc gccggcagtc attcccggca taccgacgct tgtcgtaacg aaacggataa
    33601 atataagaaa actcttagaa attttatggt aaatattgtc catgcggaca tcgacggaag
    33661 gtgacaccat gtttgacgta atcataatcg gagccggagt cgtcggcgcg aatatcgccg
    33721 cggaaatggc aagatacgat ttgaagatcg cggttttgga aaaacattcg gatgtttgcg
    33781 aagaaacctc gaaagcaaat tcgggcattg ctcacagcgg atacgacgcc ctgcccggaa
    33841 ctttgaaggc aaaatacaat gttatcggaa acaagatgat gaaagatttc tgtgaaagac
    33901 tgcagatacc gtttaaacag aacggttctt taatcatttc ccttactgac gaagggattc
    33961 ccaaattaga ggaactgtat gatcagggaa tcaaaaacgg agtcgaacaa ttgagaatca
    34021 tcggcaagga agaaatcttt tccctggaac caaatctaaa tcccgaagtg aaacatgcct
    34081 tgtatgcacc gacgggagga atcgtcgatc ctttccaatt aacgatcggt ccggcggaaa
    34141 ttgcctgcca gaacgggacc aaatttttct ttgataccga agtcacgaat attcgcaaag
    34201 agaacggaca ttttatagta acgaccaata aaggtgattt tcaaaccaaa gccatcgtca
    34261 atgccgccgg cgttttcagt gacgacatca acaactggat ttcagaacac aagaagaaga
    34321 taatgccgag aaaaggcgaa tattgcctat tcgacaaaga agtgggagat atggtgcatt
    34381 ccaccatttt ccaattgcct acaccgaaag gcaaaggcgt tctcgtcact ccgacagcgg
    34441 aaggaaattt gcttgtgggt ccgaactcgc tcggaaaata tgacaaggaa gacaccatga
    34501 cttcggctga aggtttggac tatgtcttgg aaaccgcttc gcaaagcgtg gcaaaagttc
    34561 caaccaaatt catcatcacc ggatttgccg gcctgcgtgc ttcggaagaa ggcggcgact
    34621 tcgttttggg tgaagccgaa gatgttcccg gattctttaa cgccctcgga attgaatcac
    34681 cgggtctgac agcggcaccg gccattgcga tcgacatggc aaaatgggtg gccacatatc
    34741 tccatgcaaa tcttcgtgaa aattatatcg acaaacgcga gaaacttgtc cggttcgagc
    34801 aattaggcaa cgcggaaaga caaaaattgt ttctttctga cccggaatac ggacatgtga
    34861 tttgccgttg cgaactagtg acattggcgg agataaagaa cgctattcat cgcccgcttg
    34921 gagccacaac cgtcgacgga atcaaacgga ggacccgtgc tggcagcgga cgctgtcaag
    34981 ggggtttctg ttctccgaga gtgctggaaa tcctagcgca ggagcttaaa aaggatccac
    35041 gtgagataga aaaattcagt gaaaaatcca cgtaccttgt cggtttcgac aaggacatgt
    35101 tgtgaggtgg ccgccatgtt tgaaaaagat ttagtgatta tcggcggtgg accggccgga
    35161 ttggccgcag ctgtagccgc tcacaaaaac ggagtaaagg acatattgat aatcgaaaga
    35221 gagaatcatc tcggcggcat tctgaatcag tgcatccaca acggcttcgg ccttcatact
    35281 ttcaaagaag aacttaccgg tcctgaatat gccaaaagat acgttgatca ggtgctagaa
    35341 gaaaagattc cttatatctt ggacacgatg gtgatcgatc ttgatcatga caagaaaatc
    35401 acggcgataa atgaaaccga cggtatcatg gaaatcaaag caaagtccat cattttggcg
    35461 atggggtgcc gtgaaagacc gcggggagcc ctgaatattc cgggatatcg gccgtccggt
    35521 attttctcgg ccggaacagc ccagaaattc gtcaatatcg acggatatct tcccggaaga
    35581 aatatcgtta ttctcggttc gggggacatc ggtctcatta tggcgagacg tttgacttta
    35641 gaaggcgcca atgtgaaaat ggtcttggag atcatgcctt attcgggcgg actgaaaaga
    35701 aacatcgttc aatgtctcaa tgattacaac atacctttac ggctgagtca tactgtcaca
    35761 aatatatacg gccgtgaccg cgtcgaaggc gtgacggtca gcgccgttga tgaaaacatg
    35821 aagccgattc ctggttccga ggaatatgta gagtgtgata ctttgttgtt atcggtcgga
    35881 ttgattccgg aaaacgaact ttcccggaaa gccggagtgg agataagtca gaagacccag
    35941 gggcctgtcg tttccaattc tttggaaaca tcgattcccg gaatctttgc ctgcggcaat
    36001 gtcctgcatg tgcatgattt agtcgattat gtcagcatgg aagccgaagc agccggggaa
    36061 tatgcggcca agtatttaga agggcttccg gcagtcaagc gccaaacagt gatccaaacc
    36121 ggaaacggtg tgagatacac ggtcccgagt tatgtggaca aaggttcgtt aacgggctcc
    36181 ttgacggtca gattccgggt gtccggggta tatgaaaata aatatatttc ggtttatttc
    36241 gataccaagc gactttatca taagcaaaaa cgcgttttga cacccgggga aatggaacaa
    36301 gtgctgttac cgaaaatttg gtttgacaac gtatccgact tgaagacgat tacgatcgtt
    36361 gtggaagagg agtgattagc gtggcaaagg aaaaaacttt gacttgtatc aattgtccgc
    36421 tcggatgcca gttgtcggta aaaatcgacg gcgataaaat cgaggtttcc ggattcagtt
    36481 gtccgcgcgg ggaaaaatac gccaaagaag aagtgttgca tccggttagg gtgatcacat
    36541 ccacaatacc ggtcgttaac ggagaagtcc cgatggtttc cataaaaacc aaaggcgaga
    36601 ttccgaaaga caagatcatg gaatgcatga tttcgctcaa aggattgaaa gcgaaggctc
    36661 ccgttcatga aggagacgtg atccttgcga atgtctgcaa cactggcgtc gatattatcg
    36721 ccaccaaaga ggtaaaggcg atttgaaaaa gatatttgtc atcaagacaa aatacagaaa
    36781 tttccaaatt gtgatcgaag acggatttct gctttcaatc gacacgacat cagaaaacga
    36841 aactccgccc gaacttttgg atggaccggc caaaatagtc cttaggcaat tggaggagta
    36901 tttttccaaa aaccggaaaa atttcgatat cccctacaaa atgaaaggga cattattcca
    36961 gcaagacgtt tggaaagctc tgcatcaaat cccatacgga aaaaccgcga gttattcttt
    37021 gattgctgaa atgtccggac acaaaaaagc cattagggca gtgggaacgg cccttaagaa
    37081 caatccgctt ccgatcgtaa ttccctgtca ccggataatc aggaaaaacg gattaaccgg
    37141 caattataac ggcggagccg atatgaaaaa atatttattg gaattagaaa agaacggttc
    37201 ttaaaaaaga accgttttct tttttcggga ttgacaaata caacggcgga ggatataatg
    37261 ttatacgaag ctaacatatc gcattttttt tgctagttat gttagcgaaa gctaacaaaa
    37321 ggagaaacaa tcatgacact taaagatgtg aagccgggag aaaaatgcgt gataaagaac
    37381 atctttgtca acaaccttag aaaaaggatt atcgacatgg gacttacgac tggtacaatc
    37441 gtcgccgtga aacggatggc acccctgggc gatccgatgg aaattagggt cagaggatat
    37501 tcgctgacat taagaaaagc ggaagcaagg atgatcgatg tcgaacttgt cgaaggaaac
    37561 ggcaaatgaa aaagatcgct ttgctcggaa atccgaactg cggaaaaacg acgctcttca
    37621 attctttgac cggatcgaat caatcggtcg gaaactggcc cggagtgaca gtggagaaaa
    37681 aatccggttt tttgaaacga gacaatttca tggcggagat aatcgatctg ccaggaatct
    37741 attccctgtc caccatctct ttggaagaag agatagccgc acgtttcgtc tacggaaaaa
    37801 acatggattt gattatcaac atcgtcgatg cttcgaatct tgagaggaat ctgttcctga
    37861 catatcaatt acttgatgcc ggcgttccga tcatcgtcgc cctcaattgc atggacatta
    37921 tcgaaaaaca ggaagatttc atcgatgcat ttgccatgga aaaggaactg ggagtaccgg
    37981 tggtcccgat cgtcgcttgt cgtaaaaacg gcaccgagga actgattgac aaaataatca
    38041 atttcgatta tacggtttca tcaacttcgc atttgtacgg ggagaaagtc gaaaagacga
    38101 ttaaagagtt cgccgaagcg cttggcggcg accgtttcgc ggccattcgt tttttcgagg
    38161 acggacttaa atccctggat cttaaggaaa taaaactgga caaggaagaa acggcaaaac
    38221 ttttggattc atatgcaaaa aaccgtaagg aatacgatat cgatttcgat atggttattc
    38281 cgaacgaccg ttatgatcag ataatcaaat tatgcaaaaa ggtatatcgt aagaatcctt
    38341 tggaaacgga atcgaaaacc gataagatcg atcgggtgct gaccaacaga tggctgggac
    38401 ttccgatatt tgcggctatc atgttcggag ttttctatct tgcattcgga ccggtaacct
    38461 catatatcac cgtgtggttt gttaaaggac ttgattggtt gttttcctat atcgaagatt
    38521 ttctggcatc agccggcgca gccgcttggg tttccagcat gttggaaaac gggattttcg
    38581 gcggactatc cgccgtactc ggtttcttgc cccagcttgt ggtgttgttt ttgttccttt
    38641 cgattttgga agattccggt tacatggcaa gagcggcttt cattatggac cgggctctga
    38701 gaagattcgg tctttcgggt aaatctttca ttcccatgct gatagggttc ggctgttcgg
    38761 tgccagcgat ggcatctacc aggacgcttg accgcgatga tgatcgaaag gtgacgacga
    38821 tgatcattcc tttcgtttca tgcggagcca aggctccgat ctatggcgtg tttgcgggag
    38881 ccttgttttt cggttattcg tatctgattg ttttttccat gtacatactt ggcttgctgg
    38941 ttgcgattat gtctgcgatc cttttcaaga aaaccatttt caaaggaaca agcgcgaatt
    39001 atttgatgga actcccggaa tatcggatgc cgacttttaa aaatacgatg cttcatacct
    39061 gggaaaggtc caaggctttt ttaatcaaag ccggaaccat cctgctggga gcattcatca
    39121 tcatatggtt tttaagttat ttcggtttca aggatggagt gttcggcttg cttgacgtgt
    39181 ctgatctgga gtacagtttc ctagggtatt tcggcaagtt catcttgccg gtattccgtc
    39241 cgatcggatt tacgtcctgg cagcctactg ttgctatttt gactgggatc gtggctaaag
    39301 aatcggttgt ggggacgctt ggcatccttt acggggtaac cggtaacgcc atcgagaacg
    39361 gatcattgct ttatgagtcg atccgttcgg ccttcacacc ggcgcaagcc tatgctttta
    39421 tgactttcgc tttgttggca tctccttgtg tcgccgccct ttccgctatg cgaaaggaac
    39481 tggcttcgaa aaaatggttc tggttcacgg ttttttatga actgattgtt gcttatctgg
    39541 tatcactgat tatttatcag gtaggatcac tcggtgccgg cggagttatt acagccattg
    39601 ttgcaataac agcggcgttg attgtgtttt tcacgattaa gcgtataata agaaccaa
//
LOCUS       DAOPJL010000009        36233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1234_length_36233_cov_10.827962, whole genome shotgun
            sequence.
ACCESSION   DAOPJL010000009 DAOPJL010000000
VERSION     DAOPJL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 36233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36233
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_36233_cov_10.827962"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLP02_02160"
     CDS             complement(<1..325)
                     /locus_tag="PLP02_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42299.1"
                     /translation="MGWLIDATTIIGTIVSAGGLGYAYIINKKTKSLKDSLDDMKHDV
                     QFDYLYKDSLIKLVSIQKSLDQDNYPVEKLSSELTEQLTILSVYDCMFIDKMNEEITN
                     MIKALK"
     gene            complement(506..581)
                     /locus_tag="PLP02_02165"
     tRNA            complement(506..581)
                     /locus_tag="PLP02_02165"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(546..548),aa:Phe,seq:gaa)
     gene            complement(585..660)
                     /locus_tag="PLP02_02170"
     tRNA            complement(585..660)
                     /locus_tag="PLP02_02170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(625..627),aa:Asp,seq:gtc)
     gene            complement(663..739)
                     /locus_tag="PLP02_02175"
     tRNA            complement(663..739)
                     /locus_tag="PLP02_02175"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(703..705),aa:Met,seq:cat)
     gene            complement(816..891)
                     /locus_tag="PLP02_02180"
     tRNA            complement(816..891)
                     /locus_tag="PLP02_02180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(856..858),aa:Ala,seq:tgc)
     gene            complement(901..987)
                     /locus_tag="PLP02_02185"
     tRNA            complement(901..987)
                     /locus_tag="PLP02_02185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(951..953),aa:Leu,seq:taa)
     gene            complement(993..1068)
                     /locus_tag="PLP02_02190"
     tRNA            complement(993..1068)
                     /locus_tag="PLP02_02190"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1033..1035),aa:Lys,seq:ttt)
     gene            complement(1107..1182)
                     /locus_tag="PLP02_02195"
     tRNA            complement(1107..1182)
                     /locus_tag="PLP02_02195"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1147..1149),aa:Thr,seq:cgt)
     gene            complement(1186..1278)
                     /locus_tag="PLP02_02200"
     tRNA            complement(1186..1278)
                     /locus_tag="PLP02_02200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1240..1242),aa:Ser,seq:tga)
     gene            complement(1306..1382)
                     /locus_tag="PLP02_02205"
     tRNA            complement(1306..1382)
                     /locus_tag="PLP02_02205"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Ile,seq:cat)
     gene            complement(1389..1465)
                     /locus_tag="PLP02_02210"
     tRNA            complement(1389..1465)
                     /locus_tag="PLP02_02210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1429..1431),aa:Met,seq:cat)
     gene            complement(1518..1594)
                     /locus_tag="PLP02_02215"
     tRNA            complement(1518..1594)
                     /locus_tag="PLP02_02215"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1558..1560),aa:Ile,seq:gat)
     gene            complement(1706..1984)
                     /locus_tag="PLP02_02220"
     CDS             complement(1706..1984)
                     /locus_tag="PLP02_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPF42300.1"
                     /translation="MKELLKKIIKFRDERGWKKHDTPEALAKSIVIEAAELLENYQWP
                     DTDPDMENVREELADVLMYALAMAADLGMDPEEIILEKLRKNIDKYPL"
     gene            complement(1944..2171)
                     /locus_tag="PLP02_02225"
     CDS             complement(1944..2171)
                     /locus_tag="PLP02_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HPF42301.1"
                     /translation="MENDIKLGDIVTLKKGHPCGENRWEIIRFGADCKIKCLGCGRIV
                     LIDRPTLKKRIKKVLGNINEGTAEENNQISR"
     gene            complement(2173..3027)
                     /locus_tag="PLP02_02230"
     CDS             complement(2173..3027)
                     /locus_tag="PLP02_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPF42302.1"
                     /translation="MPKTTLVKRGLGDLLAENEIVDLDKEEVVDIEISKITPNPFQPR
                     KHFDTEAMNELAESIRINGVLQPVIVKKIADGYMLVAGERRCKASEMAGFSTVPAIVR
                     DYNNQYLAELALLENIQREDLTIVEEAKAYQNAIESLHLTHLELSRKIGKSRSYVSNT
                     LGILSLPDSIQDEINTGNISMGHARALSKLKDVNRIKTIAKMVVEQNLTVRDIENLVK
                     KEKKRNEINRQPVNEELKTELLEVKTELENKYGIQNKVKVSSNKLTITFQNENELAEF
                     RKKLSGDN"
     gene            complement(3020..3793)
                     /locus_tag="PLP02_02235"
     CDS             complement(3020..3793)
                     /locus_tag="PLP02_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPF42303.1"
                     /translation="MSTVLSIANQKGGVGKTTTAINLASSLALLGKKVLLVDLDYQAN
                     ATTCMGIDRGSFEKCVFDCLIEEATIPEITVTTENPLIDVLPAKTTVEAIEEKIIRNT
                     NRDFILHTQLENVKNSYDYVLIDCPPSLGYVTMNALMASDGVIVPVQCEFLAMDGLTQ
                     LLNTIRLIQSKKKVNKETLTIFGVLLTMLDSRTTSGYQVVNEVKTYFGEKVFSTIIPR
                     NVSCSNATFYGVPVTEFSPKSKSSLSYKQLAKEVIQRNA"
     gene            complement(3797..4996)
                     /locus_tag="PLP02_02240"
     CDS             complement(3797..4996)
                     /locus_tag="PLP02_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42304.1"
                     /translation="MKQFFRRLMDTEFLPMSEVRKIKVILVMAFLLLIAVLSVPFSIF
                     FTYSLAVKIIVIAALVIGYALMIGLLQLGKILPAIHLSIIYSISLTLFYTQGTSGIYA
                     YLFFYISLTIIIFYQELYSYLLYGTLVTGIGVWYIITHQAALVMATDVPGAIYIYVII
                     LVLFYLIFLIQILYNEKMYTDMNYDWVKINKMVDKYQEDTYIYLDELRKQAKKPALHE
                     DTDFQKAVDELIVFIGEQVKESGKDIVNVFDLYLYIHERGLEKILENEEISVAMKKTA
                     NKLDKYLLNRRTDMISMILNFFLQFKKTPEYDPERYQYNLGKLAYDNDGQIIAVAMLF
                     QYLAREVTGKDEWDQVRKVLSLEEIDSLFSSLEAEEFLTSAQVALFKDNYDLFVKYLA
                     KNSERKE"
     gene            complement(4993..6198)
                     /locus_tag="PLP02_02245"
     CDS             complement(4993..6198)
                     /locus_tag="PLP02_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42305.1"
                     /translation="MARHKFHMFPEVDNYRETLEIKRLRVKNILVLIFIIIMMATTVS
                     YMLITGQFNMKTEVIPFIVGYFIVIVLNFTCLAYGGDNYRFYLFNKYLTTIGIFIMSI
                     ALVFIFQSPSAITALFIAYAITAFYQDIKGILLSDAFLLFSIVMFMIKYPEFMNMTNT
                     NLENIFGTVIFFVAFVLILTVSSYIIFKQKQFFYNQIALSKEAEYRNIDLLTDLQKTI
                     EGKDVDIDDYYVGLNKFLEAFSQKLGIENAFKDKTAIMLEMEKGANRIQIREKHPELS
                     DNELDSLESLLIGPGRQLRKVAVKMSRSMGLHIKKREIFSETHFKSFNHQSDDEEIRI
                     MAFVVFYVALKRGFIGLPKVGDKEIYNAVVNTDYFYFIDPRVMSIYQKNSEIFATISD
                     DHFGKKGSR"
     gene            complement(6212..6922)
                     /gene="rsmG"
                     /locus_tag="PLP02_02250"
     CDS             complement(6212..6922)
                     /gene="rsmG"
                     /locus_tag="PLP02_02250"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPF42306.1"
                     /translation="MKRGDNLAYDFQAILDESGLELKESMYASFARYYDLLMEGNAKC
                     NLTAITDKEEVYRKHFLDSLALAKVKKMSEQTLLDVGSGAGFPSLPLKIAFPKLRVTI
                     VDSLGKRIAFLEDLVMKLGLNDVSIVKGRAEEYGSKNSFDIVTARAVAKMNILTELCL
                     PFVKPGGSFLAMKSSDCHEELEKAKRGIAILGGKITDIIEYDLGIDGKRSIIIIDKIK
                     KSADIYPRLYAKISKNPL"
     gene            complement(6961..8820)
                     /gene="mnmG"
                     /locus_tag="PLP02_02255"
     CDS             complement(6961..8820)
                     /gene="mnmG"
                     /locus_tag="PLP02_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPF42307.1"
                     /translation="MIYESIVIGGGHAGSEAALALARLGKQTLLLTGNLKMVATMPCN
                     PSIGGPAKGTLVREIDALGGEMGKVADKTCLQMKMLNSSKGPAVRALRAQSDNVEYPL
                     AMLEVLRHTDNLELKEAYVTKLIVDNGKVHGVVLENKEEIRSEAVVLTTGTYMAGAIL
                     VGDQKTPGGPDNQRPSVGLSEDLRSHGIMTFRLKTGTPQRIKKNTVDYSVMEMSPGDG
                     FIHHFSHDPEYLRILDKEWPCYLIHTTKETHKIINENLHKSAMYGGLVEGVGPRYCPS
                     IEDKLVRFADKERHQLFIEPESIHLDEVYLQGFSTSMPHDIQDKMVASLPGMKNAVIA
                     KYAYAIEYDAIDSTQLKLSLESKVVENLFFAGQVNGTSGYEEAAAQGLMAGINASRKL
                     DGKTPLVLSRNEAYIGVLIDDLVTKGTREPYRMLTSRAEFRLLLRHDNADLRLTEKGH
                     EIGLIPEARYSRFLTKKEAIASEMKRLAEIEITPNAKTNALLAKYGLIPIKGKMTIRD
                     LFKRPDTDENIVKEMYPDILVYDKEIYEQINIEVKYEGYIAKALREAEKLKKDEGFLL
                     PEDIDYGKIGNLALEARSKLTKIRPVSIAQASRISGVNPADIQALLFHLKTRK"
     gene            complement(8834..9595)
                     /locus_tag="PLP02_02260"
     CDS             complement(8834..9595)
                     /locus_tag="PLP02_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPF42308.1"
                     /translation="MAVVFLDLDGTILNSGRPAEGVVDAIAMLKKNGHLPVIATGRVP
                     CLFEGLRKELGIEDYVSANGNYIYYKQKVVYERYIPKSVSERMMKSADCIGFDLAVEG
                     VEGFKAYRKKTDLVGLFSHVFHIEEPKLDQTYALNSDLLAFVLFEDWAVDEIKYMFPE
                     LVFNRSNRFGYDVNLSGGLKINGIKFLINYLGLPEDDIYAFGDGYNDIDMLAGVKNSV
                     AMGNAFPEVKTVARYVTDRVDERGVEKALRRFNLI"
     gene            complement(9624..9718)
                     /gene="ffs"
                     /locus_tag="PLP02_02265"
     ncRNA           complement(9624..9718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLP02_02265"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9764..10240)
                     /locus_tag="PLP02_02270"
     CDS             complement(9764..10240)
                     /locus_tag="PLP02_02270"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPF42309.1"
                     /translation="MDSTRNKSDEHFMRLALKEAEKAFMEGEVPVGCVIIHEGKVIAR
                     GHNRREKTGSVLSHAETEAIAKACRKIGDWRLDGYDVYVTLEPCPMCAGAMILSRIRK
                     VVYGAREPKFGAHLSQTALFDQPFNHKVEVISGVLAEESETMLKRFFQELRGQKQL"
     gene            complement(10221..10826)
                     /locus_tag="PLP02_02275"
     CDS             complement(10221..10826)
                     /locus_tag="PLP02_02275"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPF42310.1"
                     /translation="MCPKPNEGRIEVITGPMFAGKTEELIRRIRRLQYAEQNIIVFKP
                     TIDSRYADNEIVSHNNSRTKSVNIVKASEILDYVTDDTDVVAIDEIQFLDDDAVEICE
                     ELADKGVRVIISGLDRNFRGEPFGFMPRLMALAEEVTKLSAICVKCHAPATRTQRIIN
                     GKPANYDDPIILVAARDSYEARCRQCHEVPGKPNKYGFDKK"
     gene            complement(10908..11126)
                     /gene="rpmE"
                     /locus_tag="PLP02_02280"
     CDS             complement(10908..11126)
                     /gene="rpmE"
                     /locus_tag="PLP02_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPF42311.1"
                     /translation="MKKDIHPKYYKVKVTCTTCGNTFETGSTMKELKVDTCSNCHPFY
                     TGKQKFVAAAGRIDKFNKRYGIDDKDNK"
     gene            11318..12487
                     /locus_tag="PLP02_02285"
     CDS             11318..12487
                     /locus_tag="PLP02_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPF42312.1"
                     /translation="MDKKQEKVLEYHLRHQGKIKVSTFKKIRSKSDLSLAYTPGVADI
                     CKEIASDPTTAKKYTMKGRMVAIVTDGSAVLGLGNIGPLPALPVMEGKAALLYEFAGI
                     ETFPICLNAKTPDEIISAVKMIAPEFAAIMLEDIAAPACVMIERKLQELLDVPVFHDD
                     QHGTAIVVGAAMINASKLTKRPLTDLKVVVSGTGAAGSNVCRMLKMLGVKTIYAYDKL
                     GVVRKDKLMQYDFVVRELLEEGIVTDPGKNIDDLAGLVRNKDVFIGVSAPGILTPEMV
                     SGMNRDPMIFALANPEPEIRPEVAKAAGALIVASGRSDYPNQINNVLVFPGLMKGLLA
                     SQAKSVTLEMKKAAAIALASVMDEKTLDFEHVIPDPFDKRAVEAVAKAIASCVKS"
     gene            complement(12477..13043)
                     /locus_tag="PLP02_02290"
     CDS             complement(12477..13043)
                     /locus_tag="PLP02_02290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012261761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPF42313.1"
                     /translation="MDKIILASESPRRKEYFSLLGLDYEAIGANIDERANKSMAPDDL
                     VEDLAYQKAYAVFKNHRNRTVIGCDTIVYAANEILGKPADEEDAKRMLGILSGKTHFV
                     YTGVSIITEKMSRTFHNVTEVVFCDMSDEEISTYVKSGEPMDKAGAYAAQGLGSKFIK
                     EIRGDFFTVIGLPLSQIYQELKAMGIMI"
     gene            13178..14503
                     /gene="tilS"
                     /locus_tag="PLP02_02295"
     CDS             13178..14503
                     /gene="tilS"
                     /locus_tag="PLP02_02295"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPF42314.1"
                     /translation="MFKLNTDLLSRGDRLVVAVSGGIDSMVLLHCLNSLKDSFGISLY
                     VCHVNHHTREACKAEAELVEETCRQYGLPLEILDYHYSEEDNFHQASRKARYDFFRKI
                     ALKKGANKIVLAHQADDLAETILMRIVRGSSLSGYAGILNEGEYQGVKIIRPLLGVSR
                     NDIAIYQQENSVSFMEDESNLKDDYTRNRFRHHVMPPIKEENPAYTKKFAQFSQYLSE
                     AYSLVRETAEAFISRHIKFEDTFASVPANKIARENPAVRKEICKLVIDRLSQDSVEVS
                     FTQFLDLDTLACGDKTYAEIDIGNDLTVIKSYDDLYFHRYRPETIRFEFRIDGFGEYR
                     LPDGSLLSISTNNSIFDGKPHELWYNDLDSVFPITIRNRRDGDRVEMAVGSKKIKDLF
                     IQKKIPHADRDTLPVILDKQGKIIWIPRCFHQKQGEGKLCMYLSYQKGK"
     gene            14508..15050
                     /gene="hpt"
                     /locus_tag="PLP02_02300"
     CDS             14508..15050
                     /gene="hpt"
                     /locus_tag="PLP02_02300"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPF42315.1"
                     /translation="MLEKDIERVLVDETEINAIAKQIGEEITRDYQGKKPLIIGLLKG
                     CMPFMGLLIRHIDLYCDLDLMGVTSYHGGISSSGDVKITKDLDTPVVGRDIIIAEDIV
                     DTAKTIDIVRKLLLYRGAKSVEVATMLNKPAGRVLEFEPKYIGKTIPKEFVVGFGLDY
                     DEMYRNLPYVGVLKSSVYKK"
     gene            15127..17052
                     /gene="ftsH"
                     /locus_tag="PLP02_02305"
     CDS             15127..17052
                     /gene="ftsH"
                     /locus_tag="PLP02_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPF42316.1"
                     /translation="MIALLALLAISIIYSFGNMNAPVELRNDEFVEVLTNQGVSSVQV
                     NRLAGDLNYEAYKLTGTLVSGDSYVTYLTNSDELTVIRNIIYTLNTDSYNGNDVEYQY
                     APAATYNLINIIVPLLMIAGIVILIVYMMKGGANANKQAFDFGKSRAHLSKGQKTTFN
                     DVAGADEEKEELQEIIDFLKNPKKYIELGARIPKGVLLVGPPGTGKTLIARAVAGEAN
                     VPFYFVSGSDFLEMFVGVGASRVRDMFKTAKQNAPCILFIDEIDAVGRQRGTGLGGGH
                     DEREQTLNQLLVEMDGFGHNSGVIVLAATNRVDILDPALMRPGRFDRQIYVGRPDIKG
                     RVAILKVHARNKKINSSVTFDEIAKRTPGFTGADLENLLNEAALLAARENKKEIQLSN
                     IDEAIDRVLMGPAKKSFVYSPKVKNTVAFHEAGHAVIGIKLKNANIVQKVTIIPRGQA
                     GGYNLMLPEEDQSYLLTKDNLLDQITGLLGGRVAEEIMFNEVSTGAQSDLQKSTAIAR
                     AMVTEYGMSDNLGPVTYEHDTGTVFLGRDYGKDKNFSEAVAMEIDKEIRNIINSCHDS
                     ATKVIKENLDLLKLVAHYLLEVETLTKQDIDEIVGTGRLSRWDDKLAEQPETMAEDEK
                     TNPETSVPSEDPEKTEN"
     gene            17103..17351
                     /locus_tag="PLP02_02310"
     CDS             17103..17351
                     /locus_tag="PLP02_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPF42317.1"
                     /translation="MRLDKFLKTSRVFKRRTVAKEIALHQKISVNGRLAKPSTNVDKG
                     DVVTISYRDKELSFRILDVAMHASKDEAGGMYEVISEN"
     gene            17379..18365
                     /locus_tag="PLP02_02315"
     CDS             17379..18365
                     /locus_tag="PLP02_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPF42318.1"
                     /translation="MYKKIITAMALLLTSFLFLACSDTTVEYDVYVTVYPLQYVNEQI
                     MEGTGLTVGIVPGVTSHSDSVDWSPKEIIAMTKASYLFYVGANYDQYIDLQIESIFNN
                     QNVELVKLENETDYIQFIPGIYHEHDEDGAEIEDSGLLGIDPHFWISPIRVMEAAALI
                     YDKLVAFYPEYYATMSANYLALYTELQTLSDDFQTVLDIQEKPILTSTNIYGYLKNDY
                     GLSYIPISPGYHEETEQFTTQEKEEIVDEAIYYGIHHVFFEKNVTSPLSNAVFIALEE
                     SYGTTPIKFEFDILQTLTASDRDAGKNYISIMYENLSMIIEATDYQAHAGLE"
     gene            18373..19869
                     /gene="lysS"
                     /locus_tag="PLP02_02320"
     CDS             18373..19869
                     /gene="lysS"
                     /locus_tag="PLP02_02320"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPF42319.1"
                     /translation="MEEKLTEQEIVRREKAESLKEKGIDPFGSRFDRTHNTETFKEEF
                     SKYTQEELHDTDNPQTIKIAGRIMTRRIKGKAGFCHIQDQYGQVQLYVKLDNVGEDDY
                     ELFKNGDIGDIVGVYGNPMITNTGELSIKVTKYIHLTKALRPLPEKFHGLVDVEERYR
                     RRYLDLIMNDKSRETFVIRSLTIQSMRNFLNNLGYLEVETPILHPILGGANARPFVTH
                     HNTLDMPFYLRIAPELYLKRLIVGGFDCVYEIGRLFRNEGMDVKHNPEFTTIELYLAY
                     SDLEGMMELTENLFSYIAKTVLGSTTVTYLGKEINLQKGWRRVTMTDLIKEKTGIDFK
                     AITDFAVAKKLATDRGLDVPDHLYGVGHIVNLFFETYCEKELIQPTFVHSYPIEVSPL
                     TKKEPDDPRYTQRFEIFIDGCEYGNAYTELNDPIDQKERFLNQLKEKDLGNDEANEMD
                     ADFVEALEYGMPPTGGIGIGIDRLVMLMTDSYSIRDVLLFPHMKPRSK"
     gene            complement(19906..20811)
                     /locus_tag="PLP02_02325"
     CDS             complement(19906..20811)
                     /locus_tag="PLP02_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42320.1"
                     /translation="MSKKQFLISIIAIVVISVGSSVLMIVLEWNDSISSSILWGLIIL
                     LIGYTYLRYRISAPLQRFSSKFNMLVDYDLDIELAKNMAFQGMQEAPFNNIKQYYQMY
                     YSMALYYSGEYSEAIKYFHLLDLKKLNPIYQVLIFSITAYCAYEIDDQEEFDQSLDRV
                     KALETHITGRYHGYAASYVDILDSIKNIDTDPETYRVMVEKHFGKQDGYITTQLNYKY
                     RMAIYYQKVGNIEEMDKCLAFVIANGKQHHTALRAKEMFKGTVKVEDYIVKDEAPETE
                     EDSGPDNENAALPEDSGDNDTGNGE"
     gene            complement(20814..21404)
                     /locus_tag="PLP02_02330"
     CDS             complement(20814..21404)
                     /locus_tag="PLP02_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42321.1"
                     /translation="MDPLLKEYGSWHKENEEFYRELEEHDSVLYDRFLPVYEVLNYLY
                     GEITGNNLEETDDLNKIFGVGLEYINDQFQTCKLYLDKYFQNDLHVFLAYDRVVNALL
                     YLEDVRYELEENGLDANNEKIDLLIEELEAMLEDKKPVETTFSLYVDDALHDAIGEKI
                     SSFVGIIDIFVEVAETLGLQLYEENEIVIGKNLEGD"
     gene            complement(21422..22288)
                     /gene="fba"
                     /locus_tag="PLP02_02335"
     CDS             complement(21422..22288)
                     /gene="fba"
                     /locus_tag="PLP02_02335"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPF42322.1"
                     /translation="MALVSVEGMLRKAYKEGYAVGQFNINNLEWTKAILLTAEETKSP
                     VILGVSEGAAKYMTGYKVVADMVKAMVESMNITVPVALHLDHGTYEGAYAAINAGFSS
                     IMFDGSHYPIEENIAKTKELVALTKKLGLSIEAEVGAIGGEEDGTLGGGEIADPQECK
                     MVADLGVTILAAGIGNIHGKYPANWKGLNFEVLQEINEITKGVPLVLHGGTGIPDEMI
                     KKSISMGVAKINVNTECQLVFAAATRKYIEEGKDLQGKGYDPRKLLAPGVEAIKQSVK
                     EKMELFGSVGKA"
     gene            complement(22380..23816)
                     /locus_tag="PLP02_02340"
     CDS             complement(22380..23816)
                     /locus_tag="PLP02_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42323.1"
                     /translation="MNNEKNLINISKKSFLSVIIILFVLMVAAGILTKVIPAGEYARD
                     AEGAIISDDFHYLTENVGLAFWKWALAPFLVFGSSDGLTIAMISLFLLILGGAFSVMD
                     KTGGIRVIIDKLINRFKERKYTLMRLIILVFMVFGAFFGIFEESVALLPIIILLSLSL
                     GWDTMVGIGMCLLAAGFGFASGITNPFSVGIASQIAGVNLLDGVLYRLLIFGVMYLML
                     STFLVSYAKKIEKDPSRSPSFAIDSQKSKDFGELEEIPYKNKSLILKSYVIMFFSVLL
                     VVIISSLLDLFAGLSIPTIPLMALAFLIGGLLSGYLITRDFRKTLAYFLKGALSVAPA
                     VLLIMMAASIKYIITTGCVMDTILYKLSGLLSGQSPVVAILMIYGLVLSVQFFIGSAS
                     AKAYLIMPLLVPLVSIIGISKELSILAFIFGDGYTNVIFPTNAVLLIGLSIASVNYTT
                     WLKFTWKLQVATLLLTITLLIVGYYIGY"
     gene            complement(23930..25027)
                     /locus_tag="PLP02_02345"
     CDS             complement(23930..25027)
                     /locus_tag="PLP02_02345"
                     /inference="COORDINATES: protein motif:HMM:NF037701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42324.1"
                     /translation="MRNFEYIRWIGRTYTNIEEAAKYFYFTASGFETTFKGTMVKATF
                     KSSKYDIPDKMAYLVALIDTDDPFAKENRTYAIDEAKKEIVIANLENRKHTIKFLKRS
                     EASDSSLALLSIETDGRFIMCDETGDFKMQFIAASSSTGYGNLGNLSTAKSTGNSDGL
                     KGFAYLTSYMLGAEVDIFAASGWGVTRGWNTGGRISETENIPEAYDYIAITDQNTIDR
                     VIGKWDHFHYTPDVVVVNLGTNDFNSCGYENMTEEEKEKMETVFVTRYADFIHKLADL
                     FPKAHVIVAYGLMGDAAILEAPTARAVALANTRIGFERAYLFLMEAAGSNGNQFGSDY
                     HPNVGTGINVARSLAFFISRLSGRKIINEIK"
     gene            complement(25146..>25841)
                     /locus_tag="PLP02_02350"
     CDS             complement(25146..>25841)
                     /locus_tag="PLP02_02350"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPF42325.1"
                     /translation="VTDAIKALGSKPVPSAKYPIVFDRNAIAVLFSAFQRIFSADNVQ
                     KSMSLLKGKLNTKIGSDKVNIVDDPFLKKSNLSRSFDDEGVATKYKMLVEAGVLKTYL
                     HNLVTAKKDKIQPTGNGFGGSVAPTNFMLLPGQATKDELIASLSNGLYITDVQGAHAG
                     ANPVSGDFSLQAMGFVVKDGKVAEPVALITVASNFIDFLNDIEEVGNDVKTSFYGITC
                     PSVKVGPLSVSGL"
     assembly_gap    25842..25941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25942..26258)
                     /locus_tag="PLP02_02355"
     CDS             complement(<25942..26258)
                     /locus_tag="PLP02_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HPF42326.1"
                     /translation="MINVEKLFEKAKDKGFVDVQIFKTGTRDLSIEVFDGELEKYEIA
                     DTSSLTIKAIKDGKMVTFVTEVMDDEDIDMIVDAMLKNVSVITSVDEAEIYAGDEKYE
                     ELTG"
     gene            complement(26260..27657)
                     /locus_tag="PLP02_02360"
     CDS             complement(26260..27657)
                     /locus_tag="PLP02_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPF42327.1"
                     /translation="MLLTKNTIEQILEVGLSTGADFAEAFVEENYHNNFQLVSGRIEK
                     ANSSKSFGTGIRLARKLQSVYGYTNSQNPKDIIKLAEDLSKSFNDEEKTARKPLMKLE
                     TGTHHQVKKAPRDVSQEEKVSLMKKAYNAARNFDKAIQQVAINLIDSEQEVLIANSEG
                     RFVTEKRYRVRIYINAVAGNGQIMQTGTEGPGSGKGYEYFDEDIDVEAVAKEAARTAI
                     TMLGAEECPSGVMSVIINNKFGGVIFHEACGHSLEATSVAKNASVFCGKMGEKIAAPI
                     VTAIDDGTIENAWGSSNYDDEGYPQQRRVLIENGILKSYMIDLLNTRRMSGKATGSSR
                     RESYKFAPTSRMSNTFIDNGSSTFEEIIANTKYGLFAAKMGGGSVNPGTGDFNFAVSE
                     GYIVRDGKIAEPVRGASLVGNGADILLKIDMIGNNLSRAEGMCGSVSGSIPADVGQPT
                     IRVSSVTVGGKKGDK"
     gene            complement(27673..29163)
                     /locus_tag="PLP02_02365"
     CDS             complement(27673..29163)
                     /locus_tag="PLP02_02365"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPF42328.1"
                     /translation="MDLKVRFHELLKKEKAYEYAMQIIGWDSQTEAPRDCFPHRAEMM
                     GILSGELFRIASGKEMQETVYGLYDKKDALDDITSREIRKAKKQLDKIVKIPENEYVE
                     YMSLLQMAQRIWEDAKANNDYESFKGTLGKIIEYTKKFVSYYGEGKHPYDVLLDDYED
                     GMTMKDYDEFFNVLKQELVPFVRQILAAKDNHDYRFMEEKYDIDAQKKFSDYLMDVMC
                     FNRNRGLLKQSVHPFTWGTSPEDVRLTTRYIENMVFSNVFSVIHELGHATYEQQIDSK
                     WNDTYLTGGTSMGIHESQSRFYENIIGRSQEFWNTHYPKMKSLFPEQLKNVDAELAHR
                     AVNRVEASLIRVEADELTYPLHIMLRYEIERMIMTGQTNVDELPALWNDKMEEYLGIR
                     PETDSEGVLQDVHWSSGMIGYFPTYALGSAYAAQFYNAMNKDFNVLEAVKNNDMGKIN
                     AWLKEKIHQYGSSKDPKELLLSVTGEPFNPKYYINYLKDKYSKLYL"
     gene            complement(29246..30853)
                     /locus_tag="PLP02_02370"
     CDS             complement(29246..30853)
                     /locus_tag="PLP02_02370"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPF42329.1"
                     /translation="MTKFIFVTGGVVSSLGKGITAASLGRLLKNRGLKVFMQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGGETDLDLGHYERFIDENLTKNSSITTGKIYSSVIEKE
                     RRGDYLGATVQVIPHITDEIKSKLAIAARESDADVVITEIGGTVGDIESLPFLEAIRQ
                     ARRDYGFHNTMYIHNTLVPYLETTGELKTKPTQHSVKELRSLGITPDVIVLRTSHEIT
                     NAAREKIALFCDVRKEAVIQCLNVKILYEVALKFQQQNLDGIVCEHLMLDTKAADMTE
                     WKVLIERIRNLKEEVTIALVGKYVSLHDAYLSVSEALRHSGYHHSAKVNIEWINSGEI
                     NPENVKEKIGNPDGIIVPGGFGSRGTEGKIQAIKYARENGIPFLGLCLGMQMAVIEYA
                     RDVCKMEGANSTEFDPKTPYNVIDYLPEQYEGINMGGTMRLGLYRCEIEDNTLAMKAY
                     KKPETSERHRHRYELNNRYRDVLSQNGMIFSGINPETGLVEIIELKNHPWFVACQFHP
                     EFLSRPDKPHPLFCGFIHAALKRKHSQ"
     gene            complement(30963..31544)
                     /gene="rpoE"
                     /locus_tag="PLP02_02375"
     CDS             complement(30963..31544)
                     /gene="rpoE"
                     /locus_tag="PLP02_02375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HPF42330.1"
                     /translation="MADWNQLSILEIAETLLKQNDKPMTFLELFQAIGELKNLSDEEK
                     SDIISQVYADFIVSAKFVYVGDDLWDLKGRQSIDLWDKDGAYYGETTDFQEDEEETEK
                     EYEEDEFDEEIDEETDEEIDEEEEESEDEYEKEPDFDEFEEEEEEDIDYDGFEDEEAK
                     FVEDEDDIAPEEDEFDSDKYNEYMDDYEKMYDK"
     gene            complement(31565..31924)
                     /locus_tag="PLP02_02380"
     CDS             complement(31565..31924)
                     /locus_tag="PLP02_02380"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 family protein"
                     /protein_id="HPF42331.1"
                     /translation="MEKTVEFRMQIDGSIVSAFTGKGAQEGDRIIFSDDEGQTYRLQI
                     SDEEVRLYRTGKEELAILFRTGMHSGGILKTEGLEFEMNVFTRSITNKPTELVLEYDL
                     IDDNEPVARHLLTAKWQ"
     gene            complement(32017..>32237)
                     /locus_tag="PLP02_02385"
     CDS             complement(32017..>32237)
                     /locus_tag="PLP02_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPF42332.1"
                     /translation="ANEVNIREDTLDKMVKMYRKKHINRINDRTCSETEAGFYVDILS
                     NMERIGDHCNNMVINILSDSFTHDEQFY"
     assembly_gap    32238..32337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32338..32960)
                     /locus_tag="PLP02_02390"
     CDS             complement(<32338..32960)
                     /locus_tag="PLP02_02390"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HPF42333.1"
                     /translation="MTAYFMSIDWEYTTFEILGGLGIFLFGINMMSDSLKKLAGSKLK
                     MILEKTTNTPLKGIFVGILITGIIQSSSATSALVVGLVRAGLMTLPQAVGVIFGANIG
                     TTVTSVLIGLNIGHYALPIMFVGSVLIFFVHKRKIQELGKAILGFGMLFFGLETMGGA
                     LKQLVNEPAFSQMLLSVADHPILGVLVGVGTTAVIQSSSATIGILQQL"
     gene            complement(33139..34095)
                     /locus_tag="PLP02_02395"
     CDS             complement(33139..34095)
                     /locus_tag="PLP02_02395"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPF42334.1"
                     /translation="MDKQLILDKILEYKKIIIHTHIRPDGDCYGSGIGLREIIRESFP
                     EKEAYTVGETAEYLKFIGDYDVIDDSVYDGALSIVLDTGTVERIADQRYKKGDYIIKI
                     DHHLPVDDYGDFQYVDTTRPATAQIILEFYLEYANILKLNMTAAKALYTGIVTDTGRF
                     KYRSVNGDTFKDVAFLLDYGLDFSDILMTLDIKTEKEMKMKGYVLQNFEKTANGVAYI
                     KITPEVIRKFGVAIEEATANVNELSVLADCPVWILFAEYDDLSVRARLRSKGPAIDKL
                     ANEYGGGGHPLACGTNLDGWNQADDLLEKADELVRKYKENQQ"
     gene            complement(34080..35030)
                     /locus_tag="PLP02_02400"
     CDS             complement(34080..35030)
                     /locus_tag="PLP02_02400"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPF42335.1"
                     /translation="MFKDIFRLISENETIVIARHKAPDLDAYGSQFGLYYALKETFPT
                     KAIYAIGDTNHLNRFREMDTVDKVTLAKALLIILDTSVRQMMDGDFFEGAKIVVIMDH
                     HQNDSDIKNSIFYRDTEISSTSELVATFLLANGVKISKQAAFPLFSGIVGDTGRFLFS
                     TKPETFRIAALLTETGISLSSIYNAMYTESLQMKKTKIDYLSDFRISENGVGYRRNDQ
                     DFLERNNLSSQTASRGLVGQLSGIREIPIWANFTYDTDTRKILCELRSREIPIVDIAK
                     KYGGGGHLLACGCSVASWEETKLIVADLDKLLEEKQWTNN"
     regulatory      35152..35253
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            35314..>36233
                     /gene="guaA"
                     /locus_tag="PLP02_02405"
     CDS             35314..>36233
                     /gene="guaA"
                     /locus_tag="PLP02_02405"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPF42336.1"
                     /translation="MSDQTIVVLDFGGQYNHLIARRIRELGVRSVLLPHDTTLAKIRK
                     EYDVAGIILSGSYSSVRKPDFLSMDEKILFSGIPVLGICYGMQLIAKLMGGQVIPAAK
                     SEYGKQSVSIKNSESLLFRGVGQKTEVFMSHKDVVDALPKDFVIDASASDSMVAAIEH
                     VTLPIYGVQFHPEVTHTIEGMRMLDNFISLVCRTSKTWKMDAFAEEKANEIQNTVKDE
                     KVLLGLSGGVDSAVTAMLVHKAIGSNLTCVFVDHGLLRKNESDEVMHSLSGKFGLNII
                     RVDASDRFLEKLHGVTDPETKRKIIGNEFI"
BASE COUNT         9187 a         8522 c         7974 g        10350 t
ORIGIN      
        1 cctttaaagc cttaatcata ttcgttattt cttcattcat tttatcaata aacatgcaat
       61 catatacgct taggatagtc aattgctctg ttaattctga acttaatttc tctaccgggt
      121 aattatcttg atcaagtgat ttttgaatag aaactaattt gattaaacta tctttgtaaa
      181 gatagtcaaa ctgaacatca tgtttcatat catctaatga atcttttaaa gatttcgtct
      241 ttttgtttat aatatatgca taaccaagac caccagcgct aacaatagta cctataatag
      301 ttgtagcatc aattaaccat cccattttat actcctaatc gtgacttaac gtttcatgta
      361 ttatatcaca gatttattag caattaaata tacattgctg tttttttatg agtgactttt
      421 gtttgtccat atgtcgccat agcattatgt aattcataca aaatagcaac aaaaaagccc
      481 taaaagggct tgtttcaggg cttgatggtg gttcaggccg gaattgaacc ggcgacacat
      541 ggattttcag tccattgctc taccaactga gctaccgaac cagatggcgg tcccgacggg
      601 aattgaaccc gcgatctcca gtgtgacaga ctggcatgtt aaccactaca ccacaggacc
      661 attggttgcg gggggaagat ttgaacttcc gacctctggg ttatgagccc aacgagctac
      721 cagactgctc caccccgcga tatgatttga cgctatctta taatatcaaa tgctcacttt
      781 tttgtcaatg caaaatgagc cttaatatga acaattggtg gagtatagcg ggatcgaacc
      841 gctgacctcc tgcttgcaaa gcaggcgctc tcccagctga gctaataccc catagtaaaa
      901 tggtacccga ggccggggtc gaaccggcac gtcatcgctg acattggatt ttaagtccaa
      961 cgcgtctacc tattccgcca ctcgggcaaa aatggtgacc cgcaggcgat tcgaacgcct
     1021 gaccctttga ttaaaagtca aatgctctac cgactgagct agcgggtcac gtaaagcccg
     1081 aaccaaggtt cggctttaat aaaaaatggt gccgaaaaca ggaattgaac ccgtaaccta
     1141 ctgattacga ttcagttgct ctaccaattg agctatttcg gcacatggcg gagaaagagg
     1201 gattcgaacc cccgcgacgc ttgcgcgccc tgtcggtttt caagaccgat cccttcagcc
     1261 gggcttgggt atttctcctt gaaaaggatt tggttttcca agaggtggtg gacccagtag
     1321 gactcgaacc tacgaccgac cggttatgag ccggtagctc taaccaactg agctatgggt
     1381 cctaaaaatg gtagcggcgg agagacttga actctcaacc tcacgggtat gaaccgtacg
     1441 ctctatccag ttgagctaca ccgccagcat atgataggtg ccgttatgga ggaccttctg
     1501 tcattccaca aaaaaaatgg tgggcctaaa tggacttgaa ccatcgacct cacgcttatc
     1561 aggcgtgcgc tctaaccacc tgagctatag gcccacaaac atatgcactt tacagtgcga
     1621 gttatatttt actatctctc cttttttttg tcaattggta tttccccgat atggaaaatt
     1681 tccttgatat ccggtctcat ttggctcaaa gcggatattt gtcgatgttt tttcttaatt
     1741 tttccaaaat gatttcttcg ggatccattc cgagatcggc cgccatggct aatgcataca
     1801 taaggacgtc cgcgagttct tcgcggacat tttccatgtc cggatcggta tccggccact
     1861 gataattctc cagtaactcg gcggcttcta tcacgattga cttcgccaag gcttccgggg
     1921 tatcatgttt cttccaaccg cgttcatcgc gaaatttgat tattttcttc agcagttcct
     1981 tcatttatgt tacctagcac tttcttgatc ctttttttta aggtggggcg atcgatcaga
     2041 acgattctcc cacatcccag gcatttgatc ttgcaatcgg cgccgaagcg gatgatctcc
     2101 cagcggtttt ccccacaggg atgtcctttc ttcaaagtga cgatgtcgcc tagtttgatg
     2161 tcgttttcca ttttagttgt ctcccgacag tttctttctg aattccgcca gttcgttttc
     2221 gttttgaaag gtgattgtca gtttgtttga agacaccttc actttatttt ggatgccgta
     2281 tttgttttcc agttcggttt tgacttccag aagttcggtt tttaattctt cgttgaccgg
     2341 ctgccggttg atttcatttc gctttttttc ctttttcact aggttttcga tgtcgcggac
     2401 ggtaaggttt tgttcgacaa ccatcttggc aatcgttttt atccggttga catctttgag
     2461 tttgcttaag gcgcgggcgt gtcccatcga gatgttgccg gtattgattt catcctggat
     2521 ggaatccggt aaagagagaa taccgagagt attgctgaca taggaacggc tcttgccgat
     2581 tttgcgggaa agttccaggt gggtgagatg caaagattcg atggcgttct ggtaggcttt
     2641 agcctcttcg acgatcgtca gatcctcgcg ctgaatgttc tcaagcaaag ccagttccgc
     2701 caaatattga ttgttatagt cacggacgat ggcgggaaca gtcgaaaaac cggccatttc
     2761 ggaagccttg catcttcttt caccggccac aagcatgtac ccgtcggcga ttttcttgac
     2821 aatcaccggt tgcaagactc cgtttatccg gatggattcg gcgagttcgt tcatcgcttc
     2881 tgtgtcgaaa tgtttgcgtg gttgaaaagg gttgggagtt attttgctta tctcgatatc
     2941 gacgacttct tccttgtcta aatcgacgat ctcgttttcc gcaagcaaat ctccgagtcc
     3001 cctttttacg agagttgtct taggcattgc gttggatcac ctcttttgcc agttgtttgt
     3061 aactgaggga tgatttgctt ttgggtgaga attccgttac cggcacccca taaaaagtcg
     3121 cattggagca cgaaacattg cgcgggatga tggtactgaa caccttttct ccgaaatagg
     3181 ttttaacttc gtttacgact tggtaaccag acgtagttct ggaatcaagc atcgtcaaaa
     3241 ggacgccgaa gatggtcaag gtttctttat tgaccttttt cttgctttgg atcaggcgga
     3301 tggtgtttaa cagctgggtg agtccgtcca tggcgagaaa ttcgcattgg acgggaacga
     3361 tgacgccgtc ggatgccatc aaagcgttca tcgtgacata tccgagcgaa ggcggacaat
     3421 caatcaagac atagtcgtaa ctgtttttaa cattctcaag ttgggtgtgt aagatgaaat
     3481 cgcggttggt gttcctgatg attttttctt cgatcgcttc gactgtcgtc ttggcgggta
     3541 aaacatcgat cagcgggttt tcggtcgtca ccgttatttc cggtatcgtc gcttcctcga
     3601 tcaggcaatc gaaaacgcat ttttcgaaac ttcccctgtc gatgcccatg caggttgtag
     3661 cattggcttg gtaatctaag tccacaagaa gaaccttttt gccaagaagc gccagcgaac
     3721 tggcgaggtt gatggcggtg gtcgtttttc caacgccgcc tttttggttt gcgatagaaa
     3781 gtactgtgct cattctctat tcctttcttt cactgttttt cgccaaatac ttgacgaaca
     3841 aatcgtaatt gtccttgaag agggctactt gggccgaagt caggaattct tccgcttcca
     3901 ggctggaaaa aagcgaatcg atttcttcta acgacaggac ttttctgacc tggtcccatt
     3961 cgtcttttcc tgtgacttct ctagccagat actggaatag catggcgacg gcgatgatct
     4021 ggccatcatt gtcgtatgcc agttttccta aattgtactg atatctttcc ggatcatact
     4081 cgggagtttt tttgaattgc aagaaaaaat tcaggatcat tgagatcatg tcggtacgcc
     4141 gattaagaag atatttgtcc aatttgttgg cggtcttttt catcgccacg ctgatttctt
     4201 cgttttcaag gatcttctcc aagccgcgtt cgtggatgta tagatataaa tcgaacacgt
     4261 tgacgatatc cttgccggat tctttcacct gttcaccgat gaatacaatc agttcgtcga
     4321 cagctttctg gaaatcggtg tcttcgtgca aagcgggttt cttggcctgt tttctaagtt
     4381 cgtccaaata gatataggtg tcttcttggt atttgtcgac catcttgttt atcttgaccc
     4441 agtcgtaatt catgtcggta tacatttttt cgttgtaaag tatctgaatc aggaaaataa
     4501 gatagaaaag cacaaggatg atcacgtaga tgtagatggc tcccggaaca tccgtcgcca
     4561 tgactaatgc cgcctgatgc gtgatgatgt accagacacc aataccggtg accaatgtcc
     4621 cgtacaaaag atacgaataa agttcctgat aaaaaatgat gatggtgaga gaaatataga
     4681 aaaacaggta ggcatagatg ccgctggttc cctgggtgta aaacagagtg aggctgatgg
     4741 aataaatgat actcaggtgg atagccggaa gaatttttcc cagttgcaac aatccgatca
     4801 ttagcgcata gcctataacc agagcggcta tcacgataat ctttacagcc agagaataag
     4861 tgaaaaaaat cgaaaacgga accgacagca ccgctatcag caataaaaac gccatgacaa
     4921 gaatgacttt gattttcctg acttcactca tcggcagaaa ttcagtatcc ataagccgtc
     4981 taaagaattg tttcatctcg atcccttctt tccgaagtga tcgtcgctta tggtggcgaa
     5041 aatctcactg tttttctgat atatcgacat gactctcggg tcgatgaagt aaaagtaatc
     5101 agtattgaca acggcgttat aaatctcttt gtcaccgact tttggcaaac cgatgaatcc
     5161 gcgcttaagg gcgacataga aaaccacaaa cgccatgatc cggatttctt catcgtcgga
     5221 ctgatggttg aaactcttga aatgagtttc ggaaaatatc tctcttttct tgatgtgaag
     5281 acccatggag cggctcattt tgacggcgac ttttcttaat tggcgccccg gaccgattaa
     5341 aagcgattcc agggaatcca gttcgttatc ggagagttcg ggatgctttt cccggatttg
     5401 gatacggttt gcgccttttt ccatttctaa catgatcgcg gttttatctt tgaaagcatt
     5461 ctcgattccg agtttttggc tgaaggcctc gagaaatttg ttgaggccga cataataatc
     5521 atcaatgtcc acgtctttgc cttcaatcgt tttctgcaga tcggtaagca aatcaatatt
     5581 ccggtattca gcttctttag aaagagctat ctggttatag aaaaattgtt tttgcttgaa
     5641 aatgatatag gatgacaccg tcaaaatcag gacaaaggcg acaaagaaaa taacagttcc
     5701 gaaaatattt tccagatttg tgttcgtcat gttcatgaac tcgggatact tgatcatgaa
     5761 catcacgatc gagaaaagca agaaggcgtc gcttagcaga atgcctttga tatcctgata
     5821 gaatgccgtg atcgcataag cgatgaagag cgcggtgatc gccgaaggcg actggaatat
     5881 gaagactagc gcaatcgaca tgataaagat cccaatggtg gtaagatatt tgttgaaaag
     5941 atagaaacgg taattatccc cgccataagc aagacaagtg aaattgagaa cgatgacgat
     6001 gaaatatccc acaatgaagg ggatgacttc cgttttcatg ttgaactgtc cggtaatcaa
     6061 catgtagctt accgtggtgg ccatcatgat gattatgaaa atcaggacca ggatattttt
     6121 cacccttagt cgtttgattt ccaatgtttc ccggtagttg tcgacttcgg gaaacatatg
     6181 gaatttgtgt ctggccaaag tcaaccctcc cttacagcgg attcttactt atttttgcgt
     6241 atagccgcgg atagatatcc gcactttttt tgattttgtc aataatgatt atcgatcgtt
     6301 tgccatcaat tccgagatcg tattctatga tatctgtgat ttttccgccc aggatcgcaa
     6361 tgccacgttt ggccttttcc agttcctcat ggcaatcgga agatttcatg gcgagaaaac
     6421 ttccccctgg ttttacaaac ggcaaacaga gttcagtcag gatattcatc ttggcgaccg
     6481 ctctggcggt cacaatatcg aaactgtttt ttgagccgta ttcttcggcc cggcctttga
     6541 caatcgaaac atcattcaat ccgagcttca taaccaggtc ttccagaaaa gctatgcgtt
     6601 ttccaagact gtcgacaatc gtcactcgga gttttggaaa tgctatcttt agcggaagcg
     6661 aaggaaatcc agcgcccgat ccgacatcga gcaatgtctg ttccgacatt ttttttactt
     6721 tggcgagcgc caaggaatcc agaaaatgtt tccgatacac ttcttctttg tcggtgatgg
     6781 ctgtcaggtt gcatttggcg tttccttcca tcaaaagatc gtaataccgg gcgaaggaag
     6841 catacatgct ttcttttagt tccaaaccgg attcatcaag gattgcctga aaatcgtaag
     6901 ccaaattatc accacgtttc attttatcac aactgggggc cataaagaaa catgccccgc
     6961 ctattttctt gtcttcagat ggaaaagcaa agcctggata tcggcggggt tgactccgct
     7021 gattctggat gcctgagcga tcgacaccgg cctgatcttt gtaagcttcg atctggcttc
     7081 caatgccaga ttgccgattt tgccgtaatc aatatcttcc ggtaaaagaa agccctcgtc
     7141 ttttttcagc ttttccgctt cgcgaagcgc tttggctata tatccctcgt atttaacttc
     7201 aatgttaatc tgttcatata tctctttgtc gtatacgaga atatcgggat acatttcttt
     7261 gacaatgttt tcatccgtat ccggacgttt gaacagatcg cggatcgtca tctttccttt
     7321 tattggaatg agtccgtatt tagccaaaag cgcatttgtt tttgcgttag gtgtgatctc
     7381 tatttccgcc aatcttttca tttcgctggc aatcgcttct tttttggtta agaaccggga
     7441 atatcttgct tcgggaatca gtccgatttc atgacctttt tccgttaatc tgagatcggc
     7501 gttgtcatgc cgcaaaagca agcggaactc cgcgcgcgat gtcagcatcc tgtatggttc
     7561 cctagttcct ttggtgacaa gatcgtcgat cagaacgccg atataagctt cgttgcggga
     7621 aagaacgagc ggagtctttc cgtccagttt gcgactggcg tttattcccg ccatcaagcc
     7681 ctgagctgca gcttcctcgt aaccgctggt tccgttgacc tgtccggcaa aaaacaggtt
     7741 ttcgacgact ttggattcaa gcgaaagttt caattgcgtc gaatcaattg catcatattc
     7801 gatagcatat gcatatttgg caatgacggc atttttcatc cccggaagag aagctaccat
     7861 tttgtcctgg atatcgtggg gcatcgatgt agaaaatccc tggagataca cttcgtcaag
     7921 atggatgctt tccggttcga tgaaaagctg atggcgttcc ttgtcggcga accgaaccag
     7981 tttgtcttcg atcgacggac agtatcttgg gccgacacct tctacaagtc cgccgtacat
     8041 tgccgattta tgcagatttt cgttgataat cttgtgagtt tccttggtag tatggatcaa
     8101 ataacaaggc cattccttgt caagaatcct caaatattcc ggatcatggc tgaaatgatg
     8161 gatgaatccg tctcccggac tcatctccat aacggaataa tcgaccgtat tttttttgat
     8221 cctttgcgga gttccggttt ttaacctaaa ggtcataatg ccatgactcc gcaaatcctc
     8281 gctcaagccg accgacggtc tttggttatc agggcctccc ggagtttttt ggtctccgac
     8341 cagaatggct ccggccatat aggtgccggt tgtcaaaaca accgcttccg agcggatctc
     8401 ttctttgttt tccaaaacaa caccgtgaac tttgccgtta tcgacaatca gtttcgtcac
     8461 gtaagcttcc tttaattcaa gattgtccgt atgcctaaga acttccaaca ttgccaaagg
     8521 atattccaca ttgtcggact gggctctcaa agccctgacc gccggtcctt tagaactgtt
     8581 gagcattttc atttgaagac acgtcttgtc ggcaactttt cccatttcgc cgccaagagc
     8641 gtcgatctct ctcaccaagg tccctttggc aggaccaccg atcgatggat tgcacggcat
     8701 cgtcgccacc attttaaggt taccggtcaa aagtagtgtt tgctttccga gacgggccaa
     8761 ggcgagagcg gcttcgcttc cggcgtgtcc gccgccgatg acaattgatt cgtatatcat
     8821 caaaccaccc tccctagatc aggttgaaac gccgcaaggc cttttccact ccgcgttcgt
     8881 ccacccggtc ggtaacatat ctggcaaccg tcttcacttc cggaaaagcg ttgcccatgg
     8941 cgacggagtt cttgactccc gccaacatgt cgatatcgtt gtatccgtca ccgaaagcat
     9001 agatgtcgtc ttccggcaag ccgagataat tgatcaaaaa tttgataccg ttgatcttta
     9061 ggcctcctga tagattcaca tcataaccaa accggttgga acgattaaac actagttccg
     9121 ggaacatata cttgatttcg tcaactgccc aatcttcgaa aagaacgaac gcaagcaagt
     9181 cgctgttcaa agcatatgtt tgatcgagtt ttggttcctc tatatggaaa acatgagaaa
     9241 acaaaccgac aagatcggtt ttttttcggt aagctttgaa tccttcgacg ccttcgaccg
     9301 ccaaatcaaa tccaatgcag tcggcgcttt tcatcatccg ctccgaaacg cttttcggta
     9361 tatatctttc ataaacaact ttttgtttat agtagatgta attgccattg gcgctgacgt
     9421 aatcctcgat accgagttct tttctcagcc cttcgaaaag acacggaacc cggccggtag
     9481 ctatcaccgg aaggtgtcca ttttttttca gcatggcaat ggcgtcaact acgccttcgg
     9541 cagggcgtcc actattcagg atcgtcccat ccaaatcaag aaatactact gccataggtt
     9601 ttcctcgttt caaaaaaatc tcccatgagg catctccacc cacatagata actgacttaa
     9661 ggctgcttcc gtcaagacct gacctaattc gtaggtacct attggatgaa gatgcttcat
     9721 aagagattcc ttacggcatg attatagcat attgtcaggt tttttacaat tgcttttgcc
     9781 cgcgcagttc ttggaaaaac cgcttgagca tggtttctga ttcttccgcc agaacgccgc
     9841 taataacttc cactttatga ttgaaaggct gatcgaaaag cgctgtttgg gacagatggg
     9901 cgccgaattt cggctccctg gctccgtaca cgacttttct tatccgggaa aggatcatgg
     9961 ctcccgcaca cattggacac ggttccagtg tcacgtaaac gtcatagccg tccaaccgcc
    10021 agtcgccaat ctttcgacaa gccttggcaa tcgcttccgt ttctgcgtgc gataaaacgg
    10081 aacctgtttt ttcccgccgg ttgtgtcctc tggcgatcac tttgccttcg tggatgataa
    10141 cgcatccaac cggtacttcg ccttccatga acgccttttc ggcttctttc aaagccaggc
    10201 gcatgaaatg ttcgtcgctt ttatttcttg tcgaatccat atttgttcgg ctttccggga
    10261 acttcatgac attgcctgca ccttgcttcg taggaatccc tggccgctac taaaatgatc
    10321 ggatcatcgt aattcgccgg ttttccgttg atgattctct gggtcctggt agccggagcg
    10381 tgacacttga cgcagatggc ggacaacttc gtcacttctt cggccagagc catcagtctg
    10441 ggcatgaatc cgaacggctc gccgcggaag ttccggtcaa gaccagaaat aatgacacgg
    10501 acacccttat cagccaattc ttcacagatc tctacggcat cgtcatccag aaattggatt
    10561 tcgtcaatgg cgacaacatc tgtgtcatcg gtgacataat ccaatatttc cgatgctttg
    10621 acgatattca cggatttcgt acggctgttg ttgtgggata caatttcatt gtcggcataa
    10681 cgggaatcga tggtcggttt gaagacgatt atgttctgct cggcatactg taggcgtctg
    10741 attctgcgaa tcaactcctc tgtttttccg gcgaacatcg gtccagttat tacttcgatt
    10801 cgtccctcgt ttggtttagg gcacattcat ctttccccct ctcggattca caaaaacatt
    10861 atacaaaagt gcaacaaaaa aagccatagt ctatggctct ttttttgtca tttgttatcc
    10921 ttgtcatcga ttccgtaacg cttgttgaac ttgtcaatgc gtccggccgc ggccacgaac
    10981 ttctgctttc cggtatagaa aggatggcaa ttcgagcatg tatcgacttt cagttctttc
    11041 atcgtcgatc cggtttcgaa tgtgttaccg catgtcgtac aggtaacttt caccttataa
    11101 tatttcggat ggatgtcctt tttcatgttt tctccttctg ccatgatttt tatttcacat
    11161 catagtttgt ttcacgagca aaggtattat atcaaacatc atatcaaaaa gcaagcataa
    11221 tatttatggc ttttccgccc ttaaagcaac gaaacgtcgc tatggatgcg tgcttcatgt
    11281 gttataataa acatatcatg tgaaaggacc ggccgccatg gataaaaaac aggaaaaagt
    11341 ccttgaatac cacttgcggc atcaaggcaa gattaaggtt tccacattca aaaaaatccg
    11401 atcgaaatcc gatctttcgc tcgcctatac tcccggtgtg gctgatatct gcaaggaaat
    11461 cgctagcgat ccgacaacgg caaaaaaata tacaatgaaa gggcggatgg ttgccattgt
    11521 aaccgatggc agcgccgtgc tcggtctcgg aaacatcgga ccattgcccg ctttgccggt
    11581 catggaaggg aaagcagcgc ttttatacga gttcgccggc atcgaaacgt ttccgatctg
    11641 tttaaatgcg aaaaccccgg atgaaatcat cagtgcggtt aaaatgattg ctcccgaatt
    11701 cgcagccatc atgttagaag acatcgctgc tcccgcttgc gtaatgatcg aaagaaaact
    11761 acaggagttg ttggatgttc cagttttcca cgacgatcag cacggaacgg caatcgttgt
    11821 cggagcggca atgatcaatg cttccaaact caccaagcga ccgttaacag atttgaaagt
    11881 cgtcgtttcc ggtaccggag ccgccggcag caatgtctgc cggatgctta agatgctcgg
    11941 agtcaaaact atctatgcct atgacaaact cggggttgtc agaaaagata agctcatgca
    12001 atatgatttc gttgtcaggg aattgcttga ggaagggatc gttactgatc ccggaaaaaa
    12061 cattgacgat cttgctggtc ttgtcagaaa caaagatgtt ttcatcggag tcagcgctcc
    12121 tggcatcctg accccggaaa tggtcagtgg tatgaacaga gatccgatga tcttcgctct
    12181 tgctaatccg gaaccggaaa ttcgcccgga agtggcgaaa gctgccggag ctttgatcgt
    12241 cgcatccgga cgcagtgatt atccgaacca gataaacaat gttctggtgt ttcccggatt
    12301 gatgaaaggc ttgctggcgt cacaggcgaa atccgtgact ttggaaatga aaaaagccgc
    12361 ggctatagcc ttggcttcgg taatggatga aaaaacactc gattttgaac acgtgattcc
    12421 ggatccgttt gacaaaaggg ccgtcgaggc agttgccaag gcgatcgcct cttgcgtcaa
    12481 atcatgattc ccatggcttt gagttcctga tagatttgcg agagtggcaa tccgattaca
    12541 gtgaagaaat caccgcggat ttccttgatg aatttcgatc cgagtccttg ggcggcatat
    12601 gcacctgctt tgtccattgg ttcgccggat ttcacatatg tcgaaatttc ctcatccgac
    12661 atatcgcaaa aaaccacttc ggtcacatta tggaaagtcc gtgacatttt ctcggttatg
    12721 atggatacgc cggtataaac aaaatgggtt tttcccgata atattccaag catccgttta
    12781 gcgtcttctt cgtctgccgg cttccctaaa atctcgttgg cggcataaac gatggtatca
    12841 catccgatga cagtgcggtt gcgatggttt ttgaaaacgg catacgcttt ctgataagcc
    12901 aaatcttcca ctagatcgtc gggcgccatg ctcttgttcg ctcgttcatc gatgttcgcc
    12961 ccaatagctt cgtaatccaa acctaggaga ctgaaatatt cttttcttct cggcgattcg
    13021 ctagccagaa ttattttgtc cattttacac ctctttgctt tcagaacaaa ttataccaca
    13081 agattaaacg ggcgcccacg gtttttgacg ccccacatat tgacatcaca ttttatggat
    13141 ataatcatta caatctgtcg aaaggtaggt gacagacgtg ttcaaactca ataccgattt
    13201 gctttcccgc ggcgatcgct tggttgtcgc cgtcagcggc ggcattgatt caatggttct
    13261 tcttcattgt ctcaattccc tgaaagattc gtttggaatc tcgctttacg tctgtcatgt
    13321 aaaccatcac acaagagaag cttgcaaagc cgaggccgaa ctagtcgaag aaacgtgccg
    13381 gcaatacgga ttgcctttgg aaatactcga ttaccattat tctgaagaag acaatttcca
    13441 ccaggcttcc agaaaagccc gctatgattt ctttcggaaa atcgctttga aaaaaggagc
    13501 gaataaaatc gttctggctc atcaggctga cgatctggcg gaaacgatcc tgatgcggat
    13561 cgtccgggga agttcgcttt ccgggtatgc cggcatcctc aatgaaggcg aataccaagg
    13621 cgtcaaaatc attcgcccgc ttctgggtgt ttcgcgtaac gatatcgcca tataccaaca
    13681 ggaaaattcc gtttctttca tggaagacga atcgaatctc aaggacgatt acacgcgcaa
    13741 ccgttttcgg caccacgtaa tgccgccgat taaagaggaa aacccggctt ataccaaaaa
    13801 gttcgctcaa ttctctcaat acctgagtga agcatactcg cttgtcagag aaactgccga
    13861 agctttcatt tcccgacaca taaagtttga ggatacattc gcctctgtgc cggcaaacaa
    13921 aattgcccgg gaaaatccgg cagtcagaaa agaaatatgc aaactagtca tcgaccggct
    13981 ttcgcaggat tcggtcgaag tatcctttac tcagtttctg gatctcgaca ctttggcttg
    14041 cggcgacaag acttatgccg aaatcgacat cggaaacgat cttaccgtca tcaaaagtta
    14101 tgacgatctg tattttcacc gttacaggcc ggaaacgatc cggtttgaat tccggatcga
    14161 cgggttcggg gaataccggt tgccggacgg atccttgctt tcgatttcca caaataacag
    14221 catttttgat ggcaaaccac atgagttatg gtataatgat ttagattcag tatttcctat
    14281 taccatcaga aaccgtcgcg atggcgaccg ggtggaaatg gcagtcggca gcaagaaaat
    14341 caaggacctt ttcattcaaa aaaagatccc ccatgccgat cgcgacacct tacccgtaat
    14401 tttggacaaa caaggaaaga ttatctggat ccctcgctgt ttccatcaaa aacaaggcga
    14461 aggaaagctg tgcatgtatc tatcatatca gaaaggaaaa taataaaatg ctggaaaaag
    14521 acattgaaag agtcctggtg gacgaaactg aaatcaacgc tatcgccaaa caaatcggtg
    14581 aagaaatcac cagagactat caaggaaaaa aacccctcat catcggccta ttgaaaggat
    14641 gtatgccttt tatgggtctt ctcatcagac acattgacct ctattgtgac ctcgatttga
    14701 tgggtgtcac cagctatcac ggcggcattt cctcttccgg tgatgtcaag atcaccaaag
    14761 atctcgacac tcccgtggta gggcgcgata tcattattgc cgaagatatc gtcgacaccg
    14821 ccaaaaccat cgacatcgtc agaaaactcc tgctttaccg aggagccaaa tcggtcgaag
    14881 tggcgacgat gctgaacaag cccgccgggc gggtcttgga atttgagcca aaatacatcg
    14941 gtaagacgat tcccaaggaa ttcgtcgtcg gtttcggtct cgattacgat gaaatgtatc
    15001 gcaatttgcc gtatgtcgga gtcttgaaat cttccgtata caagaaatag cattgataag
    15061 gagaaacaaa tggcagacaa cagacagcca gtaagaaaac agacaaaccg cgcatcccat
    15121 cttctgatga ttgcgcttct ggccttgctg gcgatatcca tcatctacag ttttggcaac
    15181 atgaatgctc ccgtggagtt acgcaatgac gaattcgtcg aagtcctcac caaccaaggc
    15241 gtatcgtccg tccaggtcaa ccgcctggcc ggggatctca actacgaagc ctacaaactc
    15301 accggaaccc tcgtttccgg cgacagttac gtgacctatc tgacaaacag tgacgaattg
    15361 acggttatcc gtaacattat ttatactttg aacaccgatt cctataacgg aaacgacgtt
    15421 gaataccagt acgccccggc ggccacttac aatctgatca atatcatcgt acccttgctt
    15481 atgatcgccg gcatcgtcat tctgatcgtc tatatgatga aaggcggagc caatgccaac
    15541 aagcaggcct tcgatttcgg aaagagccgt gcccatttaa gcaaaggtca gaaaaccaca
    15601 ttcaacgatg tggccggagc tgacgaagaa aaagaggagt tacaggaaat catcgacttc
    15661 ctgaaaaacc cgaaaaaata tatcgaactc ggagccagga ttccgaaagg cgtcctgttg
    15721 gtaggccctc cgggaaccgg aaaaaccctg attgccagag ccgtggccgg cgaagccaac
    15781 gtacctttct attttgtcag cggatccgat ttcttggaaa tgttcgtcgg tgtcggcgcc
    15841 agccgagtcc gcgacatgtt caaaacagcc aaacaaaacg ctccctgtat tcttttcatc
    15901 gatgagatcg acgccgtcgg cagacagcgt ggaacggggc ttggcggcgg acacgacgaa
    15961 cgcgagcaaa ccctgaacca actgcttgtt gaaatggatg gtttcggaca caactccggt
    16021 gtcatcgtcc tcgcggcgac aaaccgtgtc gacatcctcg atccggccct catgcgcccg
    16081 ggcagattcg atagacaaat ctatgtcggc cgtcccgata tcaagggaag ggtcgccatt
    16141 cttaaggttc atgccagaaa caagaaaatc aattcttccg tcacgttcga cgaaatcgcc
    16201 aaacgtaccc cgggattcac cggagccgat ctcgagaatt tgcttaacga agccgccttg
    16261 ctggcggctc gcgagaacaa aaaggaaatc caactttcaa atatcgacga agccatcgac
    16321 cgcgtgctca tgggaccggc aaaaaaatca tttgtctaca gccctaaagt taaaaacacc
    16381 gtcgcctttc atgaagccgg tcacgccgta ataggcatca agctgaaaaa tgcaaacatc
    16441 gttcagaaag taaccatcat cccccgcgga caagcgggcg gttacaacct aatgcttccg
    16501 gaagaggacc aatcttacct gttgaccaag gataatcttt tggatcagat aaccggtctt
    16561 ctgggcggac gggtcgccga agaaatcatg ttcaacgaag tgtcgaccgg cgcccaatcg
    16621 gatctgcaaa aatcaactgc gatcgccaga gcgatggtga cggaatacgg aatgtcggac
    16681 aatctcggcc ccgttaccta cgagcacgac accggaacgg tatttctcgg tcgcgattac
    16741 ggcaaggaca agaacttctc cgaagccgtg gctatggaaa tcgacaagga aatccgcaac
    16801 ataatcaata gttgccatga cagtgccacc aaagtcatca aagaaaactt agatcttctg
    16861 aaactggttg cccattatct cttggaagtc gaaaccctca caaaacagga tatcgacgag
    16921 atcgtcggca ctggacgcct atcccgctgg gacgacaaac tggcggaaca gcccgaaacg
    16981 atggctgaag acgaaaagac aaatccggaa acttcagtac caagcgaaga tccggaaaaa
    17041 accgaaaatt aatcgttcgg ggatacgttt ttgtatcccc gttcagatat cggagggtat
    17101 ttatgcgcct tgataaattt ttgaaaacat cccgcgtatt caaacgccgg accgtcgcca
    17161 aagaaatcgc gctgcatcag aaaataagcg tcaacggacg tctcgccaaa ccgtcgacaa
    17221 acgtcgacaa gggagatgtc gtcaccatat cgtaccggga caaggaactg tcgttccgga
    17281 tattggatgt cgccatgcac gcctccaagg acgaagccgg cggcatgtac gaagtgattt
    17341 cggaaaatta aaccgaaggc gggggagact taatcgcaat gtataaaaaa ataataaccg
    17401 cgatggcgct tcttttgacg tcattcctgt ttttggcttg ttccgacacc accgtcgaat
    17461 acgatgttta tgtcacggtt tatcccttgc aatacgtcaa cgaacaaatc atggaaggca
    17521 ccggcctgac agtcggaatc gtcccgggag tcacttccca ctccgattct gtcgactggt
    17581 ctcccaagga gatcatcgcc atgacaaaag cttcttatct tttttatgtc ggagccaact
    17641 acgatcaata catcgacctt cagatcgaaa gcatcttcaa caatcagaat gtggaactgg
    17701 tcaaactgga aaacgaaacc gattatatcc aatttattcc gggtatttat cacgaacatg
    17761 acgaagatgg cgccgaaatc gaagattccg gattattggg aattgatcct catttctgga
    17821 tcagtccgat aagggttatg gaagcggccg ctttgatata tgacaaattg gtggctttct
    17881 acccggagta ttatgcgacg atgtcggcca actatcttgc cctatatacc gaattgcaaa
    17941 cactaagcga tgatttccaa accgttctcg atatccagga aaaaccgatt ctcacgtcca
    18001 caaacatata cggatatctg aaaaacgatt atggtttgtc ctacattcct atctctcccg
    18061 gttatcatga ggaaaccgaa cagttcacca cccaagagaa agaagagatc gttgatgaag
    18121 cgatctacta cggcattcat catgtatttt ttgaaaagaa tgtcacgtct cctttgtcta
    18181 acgctgtctt tatcgcatta gaagaaagtt acggaacaac tcctattaaa ttcgaatttg
    18241 acattcttca aacccttacc gccagcgaca gggacgctgg aaaaaattat atttcgatca
    18301 tgtacgagaa tctgtcaatg atcattgaag ccacagatta ccaggcacat gccggattgg
    18361 agtgacaaac atatggaaga aaaacttacc gaacaggaaa tcgtcagaag agaaaaagcc
    18421 gaaagcctta aggaaaaagg cattgatccc ttcggttccc gattcgacag aacccacaac
    18481 acagagactt tcaaggaaga gttttccaaa tacacgcaag aagaacttca tgacaccgat
    18541 aatccccaaa caataaaaat cgccggccgc atcatgacaa gaagaatcaa gggcaaagcc
    18601 ggtttttgcc acattcagga ccagtatggc caagtgcagc tttacgtcaa actggacaat
    18661 gtcggcgaag acgattacga attgttcaaa aacggcgaca tcggcgatat cgtcggagta
    18721 tatggaaatc cgatgataac caacaccggg gaactcagca tcaaggtgac aaaatatatt
    18781 catcttacga aagccttgcg gccgttgccg gaaaaattcc acggtcttgt cgatgtcgag
    18841 gaacgatatc ggagacgcta cctggatctc atcatgaacg acaagtcgcg ggaaactttc
    18901 gtgatccgtt ccctgactat ccagtctatg cgtaactttt tgaataatct cggatatctc
    18961 gaagtggaaa ctccgattct gcacccgatc ctgggcggcg ccaacgcccg tcctttcgtc
    19021 acccatcata acacccttga catgcctttc tatctccgga tcgctccgga actctatctt
    19081 aaacgtctga tcgttggcgg attcgattgc gtttacgaaa tcggacggct tttccgtaat
    19141 gagggcatgg atgtgaaaca caatcccgaa tttacgacga tcgagttata tcttgcctac
    19201 agcgatttag aaggcatgat ggagttgacc gagaatttat tcagttatat tgcaaaaacc
    19261 gtccttggca gcactacagt aacttacctt ggcaaggaaa tcaacctgca gaagggttgg
    19321 cgccgggtga cgatgacgga tttgatcaag gagaaaaccg gaattgattt caaagcgatc
    19381 actgatttcg cggtcgctaa aaaactggct accgaccgcg gtctcgatgt tcccgatcat
    19441 ttgtacggcg tcgggcatat cgtcaatctc ttctttgaaa cctattgcga aaaagaattg
    19501 attcagccga catttgtcca cagttatccg atcgaagtct cgccgcttac caaaaaagag
    19561 ccggatgatc cgcgctatac ccaaaggttc gaaatcttta tcgacggttg cgagtacggt
    19621 aacgcctaca ccgaattaaa cgatccgatc gatcaaaagg aacgtttcct gaaccagttg
    19681 aaagaaaagg atctcggcaa tgatgaagcc aacgaaatgg atgcggactt tgtcgaagcc
    19741 ctggaatacg gcatgcctcc gaccggtggc atcggtatcg gcatcgaccg gttggtcatg
    19801 ctgatgacgg atagttattc cattcgcgac gtgcttttgt tcccgcacat gaaaccacgg
    19861 agtaaataaa aaaggagtcc aagctaatgc ggactccttc tttttttatt cgccgtttcc
    19921 cgtatcgtta tcgccggaat cttccggcaa tgctgcattt tcgttatcgg gtccgctgtc
    19981 ttcttccgtt tccggagctt cgtctttgac aatgtaatct tcaactttga cggtcccttt
    20041 gaacatctct tttgccctca gggcggtatg atgctgtttg ccgttggcaa tgacaaaagc
    20101 aagacatttg tccatttctt cgatattgcc gactttttga taatatattg ccatgcgata
    20161 tttataatta agttgtgtgg ttatgtaacc gtcctgcttg ccaaaatgtt tttccaccat
    20221 cacgcgatat gtttcggggt cggtatcgat gtttttgatg ctgtcaagaa tatcaacata
    20281 agatgcggca tatccgtggt atctacccgt gatatgcgtt tccaacgctt tgacccggtc
    20341 gaggctttgg tcgaattctt cctgatcgtc aatttcgtaa gcgcagtacg ccgtgattga
    20401 aaaaatcaga acttggtaaa tgggattcag tttcttcaaa tcgagaagat ggaaatactt
    20461 gatggcttcg gaatattccc cggaataata aagagccatg gaataataca tctggtaata
    20521 ttgcttgatg ttgttgaacg gggcttcctg cattccctga aacgccatgt tcttggcaag
    20581 ttcgatgtcg agatcataat ccacaagcat attgaatttg ctagaaaatc tctgtagcgg
    20641 ggcggaaatg cgataacgca gataggtgta tccgataagc aggattatca gtccccacag
    20701 tatggaactt gaaatgctgt cgttccattc caatacgatc atcagcacgc tcgaacccac
    20761 cgatatcacg acaattgcga tgattgaaat cagaaattgc tttttactca ttgtcaatct
    20821 ccttccaaat tttttccgat gacaatttca ttttcttcgt acagctgcaa tcctaatgtt
    20881 tcggctactt cgacaaaaat gtcgatgatg ccgacaaagc ttgaaatttt ttctccgata
    20941 gcatcatgca atgcatcatc gacataaagc gaaaaggttg tctccaccgg ctttttgtct
    21001 tcgagcatag cttcgagttc ttcgattaga agatcgattt tctcattgtt ggcatccagg
    21061 ccattctctt cgagttcgta acgcacatct tctaaataaa gcagagcgtt caccacccgg
    21121 tcgtaagcga gaaaaacgtg caagtcattc tggaaatatt tatccagata gagtttacat
    21181 gtttggaatt gatcgttaat atattccagt ccaaccccga atatcttgtt cagatcatcg
    21241 gtttcttcca ggttgtttcc ggtgatttcg ccataaaggt aattcaatac ttcataaacc
    21301 ggaaggaagc ggtcgtacag gacggagtcg tgttcttcta gttcacgata aaattcctcg
    21361 ttctctttat gccaggaacc atattctttt aacaggggat ccaatttgtc gccgccgttt
    21421 ttcaagcttt tccaacagag ccgaaaagtt ccattttttc cttaacggac tgtttgatgg
    21481 cttcgactcc cggggccagc agttttctcg gatcgtatcc ttttccttgg aggtctttgc
    21541 cttcttcgat atattttctg gttgccgcgg caaaaaccaa ctggcattcg gtgttgacat
    21601 ttattttggc aacgcccatc gaaatcgact ttttgatcat ttcgtcggga atgccggtgc
    21661 cgccatgaag aacaagcgga acgcctttgg ttatctcgtt gatttcttgc aagacttcga
    21721 aattgagtcc tttccagttg gcgggatact tgccatggat atttccgatg ccggcggcca
    21781 aaatagtgac accgaggtcg gcaaccattt tgcattcttg cggatcggca atttcgccgc
    21841 cgcccagtgt tccgtcttct tctccgccga tggctccgac ttcggcttcg atcgagagac
    21901 cgagtttttt ggtcagtgct acaagttctt ttgtcttcgc gatattttct tcgatcggat
    21961 aatgcgatcc gtcaaacatg atggatgaga agccggcgtt gatggcggca taagcgcctt
    22021 cataggtccc gtggtcgaga tgcaaggcga ctggaacagt gatgttcatg gattcaacca
    22081 tggctttgac catgtcggcc acgactttgt agccggtcat atatttggct gcgccttccg
    22141 acacgcccaa gattaccggt gattttgttt cttcggcggt caaaagaatc gctttcgtcc
    22201 actcgagatt gttgatattg aactgcccta cggcatatcc ttctttatat gcttttctta
    22261 acattccctc tactgataca agcgccatat tcatatcctc cttggtatgg gatttacggt
    22321 ttaaattata ccggatttcc ggataaaagt aaaggtcgat caccaaagac tcacagtcat
    22381 caatagccga tataataacc gacgatgagc aatgtgatcg taagcaaaag tgtcgccacc
    22441 tggagcttcc aggtaaactt cagccaagtg gtgtaattca cggaagcgat acttagtccg
    22501 attaaaagaa cggcgttcgt cgggaagata acgttcgtat acccgtctcc gaaaataaac
    22561 gcaagaatcg acaactcttt ggaaataccg atgattgata caagcggaac caaaagcggc
    22621 attataagat aggccttggc ggaagcggaa ccaatgaaga actgaaccga aagaaccaat
    22681 ccgtagatca tgagaatggc cacaacaggc gattgtccag aaagcagtcc ggagagtttg
    22741 taaagaatcg tatccatgac gcatccggtg gttatgatat acttgatgct agccgccatc
    22801 atgatcaaaa gcactgcggg agcgacagaa agggcgcctt ttagaaaata agctagcgtt
    22861 ttacgaaaat cccgggtaat aaggtaaccg gaaagcaacc cgccgatcaa aaaagccagc
    22921 gccataaggg ggatcgttgg tatggaaagt ccggcaaaca ggtcaagaag cgagcttatt
    22981 atgactacga gcaataccga aaaaaacata atgacgtagg atttgagaat tagactcttg
    23041 ttcttgtaag gtatttcctc taactccccg aaatcttttg atttttgact gtcgatcgcg
    23101 aaagatggcg atcgggaggg gtctttttca atttttttag catagcttac caaaaaggtt
    23161 gagagcataa gatacataac tccgaagata agcaggcggt aaagaacgcc gtcaagcaga
    23221 ttgacgccag cgatttggga agcaataccg actgaaaacg gattggtgat cccgcttgcg
    23281 aatccgaacc cggcggccaa cagacacatt ccgattccaa ccatcgtgtc ccaaccgagc
    23341 gacaaggata aaaggatgat gatcggcaat agcgccactg actcctcaaa aatcccgaaa
    23401 aaggctccga agaccatgaa cactaggatg ataagccgca tgagcgtgta ttttctttcc
    23461 ttaaaccggt tgatgagttt atcgatgatt actctgattc cgccggtttt gtccatgacg
    23521 gaaaacgctc ctcccaagat taaaaggaac aacgatatca tggcgatagt caatccgtcg
    23581 gaacttccga aaacaaggaa gggggccaga gcccatttcc aaaacgccaa tccgacattt
    23641 tcggtcagat aatggaagtc atccgatatg atggcccctt cggcatccct ggcatattcg
    23701 cctgccggta tcacttttgt caggatgccg gcggctacca tcaaaacaaa aaggatgatg
    23761 atgacgctca ggaagctttt cttgctgatg ttgattagat ttttttcatt gttcataagc
    23821 gctccgatta tatgtggata attagtaact tatcaaatct tatcacatct tcgttctatt
    23881 ttcaacgaac agccagaaat caaagatccg gttcattttc attgcttgct cattttattt
    23941 cgttgatgat ttttcttccc gaaagacggg agataaaaaa cgccaacgat cttgccacgt
    24001 tgattccggt accgacattc ggatgataat cggaaccaaa ctggtttccg tttgagccgg
    24061 cagcttccat cagaaaaaga tacgcgcgtt caaagccgat ccgggtgttg gcaagcgcca
    24121 ccgctcttgc cgtcggcgct tccagtattg cggcatcccc catcaaaccg taagcaacga
    24181 tgacatgcgc cttgggaaac aaatcggcaa gtttatggat gaaatccgca taacgggtga
    24241 caaatactgt ttccattttt tctttttcct cttccgtcat gttctcataa ccgcaggagt
    24301 tgaaatcatt tgttccaagg tttacgacaa cgacatcggg agtgtaatga aagtggtccc
    24361 atttgccaat aactctatcg atcgtatttt ggtctgtaat cgctatgtaa tcgtatgctt
    24421 ccggaatatt ctcggtttcg gatattcttc ctccagtatt ccatcccctg gtcacgcccc
    24481 acccgctggc ggcgaatatg tcgacttcgg caccaagcat gtatgaggtc aaatatgcga
    24541 agcctttcag tccgtctgaa tttccggtgc ttttggctgt cgaaagatta cccagattcc
    24601 cgtatccggt tgatgaagaa gctgcgatga attgcatttt gaaatccccg gtttcgtcgc
    24661 acatgataaa acgcccgtcc gtttcgatcg acaacagagc aagggatgaa tcggatgctt
    24721 cgcttctttt cagaaacttt attgtgtgtt tcctattttc cagattggcg atgacgattt
    24781 cttttttcgc ttcgtcgatg gcataggtcc ggttttcttt ggcaaaaggg tcgtccgtat
    24841 cgatcaatgc gacaagatat gccatcttgt cgggaatgtc atatttggag gatttgaatg
    24901 tggccttcac cattgttcct ttgaatgttg tttcaaagcc gcttgcggtg aaataaaaat
    24961 attttgctgc ttcttctatg ttcgtatatg ttcttccgat ccaacggatg tattcgaaat
    25021 ttctcatgtt ccttctcctg ccgtgtgttt catatacaaa tgataccaca cttcctttct
    25081 taccggcaat ctttgttttt cataaaaaaa gcatcccgaa ggatgctttg gtattatgca
    25141 atcaatcaaa ggccggatac ggaaagcgga ccgaccttga cagatgggca ggttattcca
    25201 taaaaggatg ttttgacatc gtttcctact tcttcgatat cgttgagaaa atcgatgaag
    25261 ttgctggcga cagtgatgag cgccaccggc tcggccactt tgccatcctt cacgacgaat
    25321 cccatcgcct gtaaggagaa atccccgctg accggattgg cgccggcatg ggctccctgg
    25381 acatcggtta tgtataatcc gttggaaagc gatgcgatca gctcgtcttt ggtcgcctgc
    25441 cccggcagca gcatgaagtt ggtcggagcc accgaaccgc cgaacccgtt tcctgtcggc
    25501 tgaattttat ctttctttgc ggtgaccaaa ttgtgcaagt atgtcttcaa gacgccggct
    25561 tccaccaaca ttttgtattt ggtggctacg ccttcgtcgt cgaacgaacg gctcagattg
    25621 ctttttttga ggaaaggatc gtccacgatg tttactttgt cggatccgat tttcgtattg
    25681 agttttcctt ttagaagcga catgctcttt tgaacattat cggcggaaaa gattctttgg
    25741 aatgccgaaa acaataccgc tatggcgttt ctgtcaaata cgatgggata tttggccgag
    25801 ggaaccggtt tggatcccag cgccttgatg gcgtcagtga cnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn nnnnnnnnnn nccggtaagt tcttcgtatt tttcgtctcc ggcatagatc
    25981 tcagcttcgt caacggatgt gatgacgctg acattcttca acatggcatc aacgatcata
    26041 tcgatgtcct cgtcatccat gacttccgtc acaaacgtca ccatttttcc atccttgatc
    26101 gctttgattg tcagactgga agtatcggct atttcatatt tttcaagttc tccatcgaat
    26161 acctcgatac tcagatccct ggttccggtt ttgaatatct ggacatcgac aaaaccttta
    26221 tcttttgctt tttcaaacag tttttctacg ttgatcattt tatttgtccc ccttcttgcc
    26281 gccgacagtc acggatgaaa ccctgattgt cggttgcccg acatcggcgg gaatcgatcc
    26341 ggaaaccgat ccgcacatgc cttcggcgcg gctgaggttg tttccgatca tgtcgatttt
    26401 caagagaatg tccgctccgt ttccgacaag ggacgctcct cttaccggct cggcgatttt
    26461 tccatcccgg actatgtacc cttccgaaac agcgaagttg aaatcgccgg ttccgggatt
    26521 gacagatcct ccgcccattt tggcggcgaa caagccgtat ttggtattgg cgatgatttc
    26581 ctcgaaggtt gatgatccgt tatcgatgaa tgtgttgctc atgcgggatg tcggagcgaa
    26641 cttgtatgat tccctgcgtg acgatccggt ggcttttccg ctcatccttc gcgtgttgag
    26701 cagatcaatc atatacgatt tcaatatccc gttttcaatc agaacacgtc tttgctgagg
    26761 atatccttcg tcatcgtaat tggatgatcc ccatgcgttt tcgatggttc cgtcatcgat
    26821 tgccgtcacg atcggcgcgg cgattttttc tcccatcttt ccacaaaata ccgatgcgtt
    26881 tttcgcaaca gatgtggctt ccaggctatg tccgcatgct tcgtggaaaa tgacgccgcc
    26941 gaatttgttg ttaatgatca cagacattac accgctcggg cattcttctg ctcctagcat
    27001 tgtaatagcc gttctcgctg cttctttggc taccgcttca acatcgatat cttcgtcgaa
    27061 atattcgtaa cccttgccgc ttccgggtcc ttctgtgcct gtttgcatta tttgaccgtt
    27121 tccagcaaca gcgttgatat atatccggac acgatatctt ttttctgtta caaaacgtcc
    27181 ctccgagttt gcaatcaaga cttcctgttc ggaatcgata aggttaatcg ctacctgttg
    27241 gattgcttta tcgaagtttc tggcagcgtt gtatgccttt ttcatcaagg atactttttc
    27301 ttcctgactt acgtctctcg gtgctttttt tacttgatga tgagttccgg tttccaattt
    27361 catcaacggt ttcctggctg ttttttcttc gtcgttgaaa ctcttggaaa gatcttcggc
    27421 caatttgatt atgtcttttg ggttctggct gttggtgtat ccgtaaacgc tttggagttt
    27481 gcgggccagt ctgatgccgg ttccgaaaga tttgctgctg ttggcttttt caattcttcc
    27541 agaaaccaat tgaaaattgt tgtgatagtt ttcttcgacg aaagcttccg caaaatccgc
    27601 tcccgtcgaa agtccgactt caagaatctg ttcgattgtg ttttttgtca ataacattgt
    27661 ctattcctcg tttcataaat atagtttgct gtatttgtct tttaaatagt ttatgtagta
    27721 tttgggattg aacggttctc cggtgacgga aagaagcaat tctttgggat ctttgctgga
    27781 tccgtactga tggattttct ctttcaacca agcgttgatt tttcccatgt cgttgttttt
    27841 gacagcttcc agaacattga aatccttgtt catagcgttg tagaactggg cggcgtaggc
    27901 gctgccaaga gcatatgtcg ggaagtaacc gatcatgccg ctactccaat gaacatcctg
    27961 caaaacgcct tcagaatcgg tttctggacg aattcccagg tattcttcca ttttgtcatt
    28021 ccaaagcgcc ggcaattcgt cgacattggt ttggccggtc attatcatcc gttcgatctc
    28081 gtaacgcaac atgatgtgaa ggggataagt gagttcatct gcttcgacac gaatcagcga
    28141 ggcttcgaca cggttaacgg ctctatgcgc cagttcggcg tcaacattct tcaattgttc
    28201 cggaaacagc gatttcattt tcggatagtg agtattccag aattcctgac tacgtccgat
    28261 gatgttttcg taaaaccgcg attgcgattc atggatgccc attgatgttc cgccggtcag
    28321 ataagtatcg ttccatttgg aatctatttg ttgctcatag gtcgcgtggc cgagttcatg
    28381 gatgaccgag aacacgttgg agaacaccat gttttcaata tatctcgttg tcaaccgcac
    28441 atcttcgggg gatgttcccc aagtgaacgg gtgtacgctt tgttttaaca agccgcggtt
    28501 gcggttgaaa cacatgacat ccatcagata atcgctgaat ttcttctggg cgtcgatgtc
    28561 gtatttttct tccatgaacc ggtaatcgtg gttgtctttc gccgctagaa tttgtcttac
    28621 gaacggaacc agttcctgtt tcaggacgtt gaaaaactcg tcgtagtctt tcatggtcat
    28681 tccgtcttcg taatcatcca acaacacatc gtacggatgt tttccttcgc cgtaatatga
    28741 tacgaatttt tttgtgtatt cgataatttt tccaagcgtt cccttgaaag attcgtaatc
    28801 attgttggct ttggcgtctt cccagattct ctgggccatt tgaagcaggc tcatatattc
    28861 gacatattcg ttttccggaa tcttgacgat cttgtcgagt tgcttcttgg ctttgcggat
    28921 ttcccgtgat gtgatatcgt caagcgcatc tttcttatca tataatccgt aaaccgtctc
    28981 ctgcatttcc ttgccgctgg cgattcggaa aagttctccg ctcaagatgc ccatcatttc
    29041 tgctcgatga ggaaagcaat ccctgggtgc ttcagtctgg gaatcccaac cgattatctg
    29101 catcgcatat tcgtaggcct tttccttttt caataattca tgaaaccgta ctttcagatc
    29161 catatgcttt tcacctcgtt tccattataa caactatcaa ggcgcaattc cacctttttg
    29221 ttttgtaaac aaaaaacagc cattttcact ggctgtgttt tctttttagc gctgcgtgga
    29281 tgaaaccaca aaacaaaggg tggggtttgt cgggacggga cagaaactcc gggtggaact
    29341 ggcaggcgac aaaccacggg tgatttttga gttcgattat ttccaccaag ccggtttccg
    29401 ggttgattcc cgagaaaatc atgccgtttt gtgacaagac atctctatac cgattattaa
    29461 gttcatagcg atgacgatgt ctttcggatg tctccggctt cttgtaagcc ttcattgcga
    29521 gggtattatc ttcgatctcg catcggtata atcctaggcg catcgtccct cccatgttga
    29581 tgccttcgta ttgttcaggg aggtaatcaa tgacgttata tggtgttttc ggatcgaatt
    29641 cggtcgagtt agctccttcc attttgcata catcgcgggc atattcgatg acagccatct
    29701 gcatccccag acataatccc aaaaacggta tgccgttttc ccgggcatat ttgattgctt
    29761 ggatttttcc ttcggtacca cgtgatccga atcctcccgg cacaattatg ccgtctgggt
    29821 ttccgatttt ctctttcacg ttttccggat tgatctcgcc ggaatttatc cattcgatat
    29881 tgaccttcgc cgaatggtga tatccggaat gtcgcaaagc ttccgaaaca gaaagatagg
    29941 catcgtgcaa ggaaacatat ttgccgacaa gggcaatcgt tacttcttct ttcaaatttc
    30001 tgattctttc gatcaagacc ttccattcgg tcatatccgc tgcttttgta tcgagcatca
    30061 aatgttcgca aacaatcccg tcgagatttt gctgctggaa tttcaaagcg acttcgtaga
    30121 gaatcttcac attcaaacac tgaatgaccg cttcttttct gacatcgcag aaaagggcga
    30181 ttttctctct ggcggcgttg gttatttcat gactggttct taagacgatg acatccgggg
    30241 tgattcccag agaacgcagt tccttgacgc tatgctgggt tggcttggtt ttcagttcac
    30301 cggtcgtttc caaatatgga acaagggtat tgtgaatata cattgtgtta tgaaatccat
    30361 aatcgcgtct ggcttgtcta attgcttcca aaaacggtaa cgattcgatg tcgccgaccg
    30421 ttccgccgat ttccgttatg acgacatcgg cgtccgattc ccgggccgcg atcgccagtt
    30481 tggattttat ttcatccgtt atatggggaa ttacctggac tgtggcacca agataatcac
    30541 cccgcctttc cttttcgatg acggaagaat agattttgcc ggtggttatc gaactatttt
    30601 tagtcaaatt ttcatcgatg aacctttcat aatgcccaag gtcaaggtcc gtttcgccgc
    30661 cgtcatcggt cacgaaaacc tccccgtgtt gataagggga catggtcccg ggatcgacgt
    30721 tgatatatgg atcgaatttc tgcatgaaaa ccttcaaccc gcgatttttg agcaaacggc
    30781 ccaaagatgc ggcggtgatt cctttgccga gactcgatac gactccgccg gtcacaaaga
    30841 tgaatttcgt cactttcata tcatgcacct caaaaaaaca aaaaggcctc ccgtgggaga
    30901 accttttgtt ggctcttttc gattatatca aatagtcggc gcaaaagcaa acggatttgt
    30961 gactatttgt catacatttt ttcgtaatcg tccatatact cgttgtattt gtcgctgtcg
    31021 aactcgtctt cttcgggagc gatatcgtct tcgtcttcga caaatttcgc ttcttcatct
    31081 tcgaaaccgt cgtagtcgat gtcctcttcc tcttcttctt cgaattcgtc gaagtcgggc
    31141 tctttctcgt attcgtcctc ggattcttcc tcttcttcgt caatctcttc gtcggtctct
    31201 tcgtcgattt cttcgtcgaa ctcgtcttct tcgtattcct tttcggtttc ttcctcgtct
    31261 tcctggaaat cggtggtttc gccgtagtaa gctccgtcct tgtcccagag atcgattgat
    31321 tgacgtcctt tgagatccca aaggtcgtca ccgacatata cgaatttggc cgaaacgatg
    31381 aaatcagcat atacctgtga gattatgtcg cttttttcct cgtcggaaag atttttcaat
    31441 tcgccgattg cttgaaacaa ttcgagaaac gtcatcggtt tatcgttttg ttttaaaagc
    31501 gtctcagcaa tttccaatat tgataattga ttccaatctg ccatctgtag attcccttcc
    31561 gttttcattg ccattttgcc gtcaataagt gtctggctac cggctcgttg tcatcaatta
    31621 gatcatattc caataccaac tcggtcggct tgttcgtgat ggatctcgta aacacgttca
    31681 tctcgaattc caagccttcg gttttgagaa ttcctccgga atgcataccc gtacgaaaca
    31741 ggatggcgag ttcctccttg ccggtgcggt acagtctgac ttcttcgtcg gatatctgca
    31801 agcggtaagt ttgaccttcg tcgtcagaga aaatgattcg gtcgccttct tgcgcgcctt
    31861 tgccggtaaa tgcggatacg atcgaaccgt cgatttgcat tctgaattcg actgtttttt
    31921 ccaatagaaa tatcttcctt tgaatggctt aaattattat atattaatct atgtgaaaat
    31981 acaagtgcaa tattttagtt ttttttaatg tcttttttaa tagaattgct catcatgcgt
    32041 gaaactgtca cttaaaatgt ttatcaccat gttattgcaa tggtcgccaa tcctttccat
    32101 gttggaaaga atgtcgacat agaacccagc ttcagtttct gaacatgtgc gatcattgat
    32161 ccgatttata tgtttttttc gatacatttt aaccatttta tccagggtgt cttctctgat
    32221 gttgacttcg ttcgccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt
    32341 tgttgcaaga taccgatcgt ggcggaagac gactggatga ccgccgtcgt acctacgccg
    32401 accaggactc ccaaaatcgg atgatcggcc acacttagaa gcatttgact gaaagccggt
    32461 tcgttcacga gttgtttcag ggcgccgccc atggtttcaa gcccgaagaa aagcatgccg
    32521 aagccaagaa tggctttgcc aagttcctgt attttgcgct tatggacaaa aaatatcaga
    32581 acactgccga cgaacatgat cggcaaagca tagtgtccga tgttgagacc gatcagaacg
    32641 gatgttaccg tggttccgat attggctccg aaaatgactc cgaccgcttg gggaagagtc
    32701 atgagtccgg cacggaccag tccgactact aaggcgctgg tggccgaaga cgactgaatt
    32761 attccggtga tcaatatgcc gacgaaaata cccttgagcg gtgtattggt cgttttttcc
    32821 aaaatcattt tcagtttgga gccggccaat tttttcagcg aatcgctcat catgttgata
    32881 ccaaaaagga aaatccccaa accgccgagg atttcaaagg tggtatattc ccaatcgatg
    32941 ctcatgaaat atgccgtcat ccggttcctc cctgatgttt tttggatagt tcatccttgc
    33001 gaaccgctac tattatatca aatacccatg tcgattcaaa catttaaaaa cactttcatt
    33061 gctttttccg ctattcgaaa tccgaaaatg ggattatttc tttcttggtt tcttgccaaa
    33121 ggcaaactac aaaaacgttt attgctggtt ttctttgtat tttcttacca gttcgtccgc
    33181 cttttccaac aaatcatcgg cttgattcca accatcaaga ttagttccgc aggcaagcgg
    33241 gtgtcccccg ccgccgtatt cgttcgccag tttgtcaatg gcggggcctt tggaacgaag
    33301 cctggctctg accgacaagt cgtcatattc ggcgaaaaga atccagaccg gacaatcggc
    33361 aagcacggaa agttcattga catttgccgt cgcctcttcg atcgccactc cgaattttct
    33421 tattacttcc ggggttattt tgatgtaagc cactccgttt gcggtttttt caaagttttg
    33481 cagaacatat cctttcatct tcatttcttt ttcggttttg atatccaagg tcatcaggat
    33541 gtccgaaaaa tccaatccat aatcaagtaa aaaagcgacg tctttaaacg tgtctccgtt
    33601 gaccgagcgg tatttgaacc gtcctgtgtc agtcacgatt cctgtataaa gggcctttgc
    33661 ggcagtcata ttcagtttga gaatattggc gtattccaga taaaattcta gaattatctg
    33721 agctgttgcg ggcctggtgg tgtcgacgta ctggaaatcg ccataatcgt caaccggcaa
    33781 atgatggtcg attttaatta tgtaatctcc ttttttatat ctttgatcgg caattctttc
    33841 cacggtcccc gtatcgagaa cgatcgaaag ggctccgtca tataccgagt cgtcgatcac
    33901 atcgtaatcg ccgatgaact tcagatattc ggctgtttcg ccaaccgtgt aggcttcttt
    33961 ttccggaaat gattccctga tgatttccct gagtcctatt ccggatccgt aacaatcgcc
    34021 gtcaggccgg atatgggtgt gaatgattat tttcttatat tctaatattt tgtctaaaat
    34081 caattgtttg tccattgttt ttcctccaaa agtttgtcga gatcggcgac tatcagtttt
    34141 gtttcctccc aggaagccac gctgcatccg caggctagca gatgcccgcc gccgccgtat
    34201 tttttggcga tgtctacgat cggtatttct cttgatcgca gttcacaaag aatttttctg
    34261 gtgtcggtat catatgtgaa atttgcccat atcggaattt ccctgatacc agaaagttga
    34321 ccgacaagtc ctctgctggc cgtttgcgaa gacaggttgt ttctttcaag gaaatcctga
    34381 tcgtttcgcc tatagccgac cccgttttcc gatatccgga aatcagacag atagtcgatc
    34441 ttggtttttt tcatttgcaa ggattccgta tacatggcat tgtagatcga ggaaagcgat
    34501 attcccgttt ccgtcagcaa agccgcaatc cgaaacgttt ccggcttggt ggaaaaaaga
    34561 aatctaccag tatctccgac aatgccggaa aacaaaggaa aggcggcctg tttggatatc
    34621 ttaacaccgt ttgccaacaa gaaagtcgcc acgagttccg aggtggaaga tatctcggta
    34681 tcgcgataaa agatactatt ctttatatcg gaatcattct ggtggtgatc cattatcacg
    34741 acgattttgg cgccttcgaa aaaatcgccg tccatcatct gtcttacgga tgtatcgagg
    34801 atgatgagca aagcttttgc aagcgtcacc ttatccactg tgtccatttc ccggaaacgg
    34861 ttcagatgat ttgtatcgcc gatggcgtaa atagccttgg ttgggaaagt ttccttaagg
    34921 gcataataaa gaccaaactg ggagccatag gcatccaaat cgggagcttt gtgcctggca
    34981 ataacgatag tttcgttttc ggatattagt ctgaatatgt ctttgaacat gacgttcctc
    35041 ctagtgcttg atattacatt atatcacatg tgtccttcat gcatgaaaca aatcgtcatt
    35101 atcttacgag gcgccggcga ttaatcttgc ttttccccgg gatatcggat aaaataagaa
    35161 caaataaaaa ttcgtatatc gccggtgata cggaccggca gtctctaccc cggaaccgga
    35221 aatttcggga ctacgaacaa gccaatggct ttttttgttt ttcgggcgac ggagactttt
    35281 gttttccgtc gctttttttg aaaggagaga cccatgtccg atcagactat cgttgttttg
    35341 gatttcggag gacagtacaa tcatctaatt gccagaagaa tcagggaact gggcgtaaga
    35401 agcgttttgt tgccacacga cacgacgctc gcgaaaataa gaaaagaata tgatgtcgcc
    35461 ggcatcatcc taagcggcag ctatagcagc gtacgcaaac cggatttttt atcgatggac
    35521 gaaaaaatcc ttttttccgg aattcctgtt ttgggaattt gttacggcat gcaattgatt
    35581 gccaaactga tgggcggcca ggtgattccg gccgcaaaat cggaatatgg caagcaatcg
    35641 gtatccatca agaattccga atctcttcta tttcgcggcg ttggtcaaaa aaccgaagtc
    35701 ttcatgtctc acaaagacgt ggtggatgct cttccgaaag atttcgtaat tgacgcttcc
    35761 gcttccgatt ccatggttgc tgctatcgaa catgttacgc ttccgatata tggtgtccaa
    35821 tttcatccgg aagtcacgca tacaatcgaa ggcatgcgga tgttagacaa tttcatctcc
    35881 cttgtttgtc gtacttcaaa aacatggaaa atggacgcgt ttgccgagga aaaggcaaat
    35941 gaaatccaaa atacggtcaa agacgaaaaa gtgcttcttg gtttgtcggg cggagttgac
    36001 tccgcggtta cggccatgct tgtccacaaa gccatcggat ctaacctgac ttgcgtgttt
    36061 gtagatcacg gtttactaag aaaaaacgaa tccgacgaag tgatgcattc cctttccggc
    36121 aaatttgggc tcaatatcat ccgtgttgac gcctctgatc gtttcttaga aaaattgcac
    36181 ggcgtaaccg atccggaaac caaacggaaa atcatcggaa acgaattcat aaa
//
LOCUS       DAOPJL010000010        36180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1238_length_36180_cov_7.780235, whole genome shotgun sequence.
ACCESSION   DAOPJL010000010 DAOPJL010000000
VERSION     DAOPJL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 36180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36180
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1238_length_36180_cov_7.780235"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLP02_02410"
     CDS             <1..527
                     /locus_tag="PLP02_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPF42337.1"
                     /translation="AVPAVFSALLMLGVNRNAERMVAEIHRQFKEIPGLKEGKEGVSP
                     ESDKCVAIATKGALKELIPAGLFAIIMTLAVGFIGGVNAIGGFLAGNIVSGLLLALFM
                     SNSGGLWDNGKKYIEAGANGGKGSDAHKAAVVGDTVGDPFKDTAGPSLNTQITVVSLV
                     ASLMSVIFLTMSIF"
     gene            complement(570..1934)
                     /locus_tag="PLP02_02415"
     CDS             complement(570..1934)
                     /locus_tag="PLP02_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPF42338.1"
                     /translation="MDFKPLVEKQREFFRTGATKNVEFRLAALEKLKEAIVSHEGEIE
                     KALRNDLGKAPFETYITEIGLVLSEIDYFIVHLRKLARPKKVRTPIVLFKAKSYIMPE
                     PFGTVLIMSPWNYPLQLALTPLAGALAAGNTAVVKPGSYAVETCKMIKTIIEESFKPE
                     YVAVVLGGREENQQLLETRFDYIFFTGSTNIGKMVMEKASRYLTPVSLELGGKSPCLI
                     DSGVNMDLVAKRLAFGKFINAGQTCVAPDYVLIRKEDENDLVEGLKKYIRLFFGSDPL
                     LCEELPKIINQKHYERLLGLINPKKIVLGGEHANEKIAPTVMTGVTWEDDIMKEEIFG
                     PVLPIINFDSLDQAYDMIIDHEKPLAFYLFTNDRKVRKEALDRISFGGATINDTLMHF
                     GSTELGFGGVGASGMGRYHGKFSFETFSNMRSVLERSNLVDLEMRYHPYSEAKGNIAR
                     KLLK"
     gene            2037..2987
                     /locus_tag="PLP02_02420"
     CDS             2037..2987
                     /locus_tag="PLP02_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPF42339.1"
                     /translation="MIYLTGATGHLGNNIARYLQNEGIPFTILLRKNGKALLDLNDVK
                     YVFGDIFDPVFLSNNISENDTLIHSAGFIDLNNHHKKECDSANYLGTEQIIDFCQKSK
                     VRLIYISTVDCIPREKSQGFISEPESLDVDNEGSNYTVSKGKATAYLLEKMKNEKMPA
                     VILYPSAIIGINDFKPSALGKEIRKAMRRRISFYFRGGYNFIDVRDCAKAVVECVKNN
                     QSGCYILAANNRSVKQLLKEVGKVQNHKVFLIGVPVFLVRTFSFLIPDISDFMIDTMT
                     DNYNYDNSLMLRDLVPEPFTFEKTVDDTVKWFSENEHIFE"
     gene            complement(3100..3717)
                     /locus_tag="PLP02_02425"
     CDS             complement(3100..3717)
                     /locus_tag="PLP02_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPF42340.1"
                     /translation="MRIGIIGPIGSGKSTLAGKLARYYNAELVEEPVEKNEFLPLFYQ
                     DKETFALLSQNAFYSALFLLMWKSKDIDNLVCDSTLFSNLVFTELLRLEGIMSAMEVA
                     LTYAIADEHMKRIPDVDVHIVIMRRTEQLFENVRRRGRVIEKDQQEYLAFHYANYYDV
                     LKRIFKAYKIPEKKILWLSIEDMEEKEEFASIVKAVEDKYTESKK"
     gene            complement(4187..5797)
                     /gene="groL"
                     /locus_tag="PLP02_02430"
     CDS             complement(4187..5797)
                     /gene="groL"
                     /locus_tag="PLP02_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687962.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPF42341.1"
                     /translation="MSKEVRYSKEARMGMLKGVDILADAVKVTLGPKGRNVVLDKGYG
                     SPMITNDGVTIAKEIELKDAFENMGAKLVYEAANKTNDVAGDGTTTATVLTQSMIHKG
                     IEHVEKGTNPVLMREGMEKAAKEVAKKLLEKSHKIETDEDIAHVATVSSGSSEIGDII
                     AKAMGKVGHNGVISVDESKGFDTYLEVVEGLQYEKGYISPYMVTDREKMQIVMENANV
                     LVTDQKITTIKDILPLLEEIVQANKPLLIIADDIENEVLSTLIVNKLRGTFNVVATKA
                     PGFGDNQKEILTDIATLTGARFVAKDLNQEIKDVKIDDLGLAKKVIIGKDNTTIIDGS
                     GEKKKITARVDEIKKQMDRTTSDYDKKRLGERLAKLTSGVAIVKVGATTETELKEKKM
                     KIEDALNATKAAVEEGIVIGGGAALVEIYKEIKANLKSNVNDTQKGINVVVESLLAPI
                     YQIAENSGYDPLEIVELQKKAEKNYGFDAKNGVWVDMFKEGIVDPTKVTRSAIMNAAS
                     ISALFITTEVGVAEIKEEKPVAPAPEMY"
     gene            complement(5803..6090)
                     /locus_tag="PLP02_02435"
     CDS             complement(5803..6090)
                     /locus_tag="PLP02_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPF42342.1"
                     /translation="MLKPLHDNVVLKKEKVEKKTASGIILTGDVKEQPSFADVVAVGE
                     GNLVDGKLIPLTVKTGDKVVYKKYSTTEFKYDDEEYLIIAEKDILAVLEEE"
     gene            complement(6252..7322)
                     /gene="buk"
                     /locus_tag="PLP02_02440"
     CDS             complement(6252..7322)
                     /gene="buk"
                     /locus_tag="PLP02_02440"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPF42343.1"
                     /translation="MDYVQLIINTGGVSTKVAIFINDKLIQEQVLKHDVEKLKEFATA
                     MDQAEFRKQLILDFLAEHHYSVEQIDIFVGRGGLLRPLKQGGTYLVTEKMVDDLRNCR
                     YGSHAANLGAIIAYELAKPINRPAYTVNPVSVDEFNDFARVSGLKGIERRSVFHVLNH
                     KAIAKRHAKTIGKPYESMNLIVCHLGSGISVGLHEKGRVVDVNNALGGDGPFSTERTG
                     TVPTYPLVDLCFSGKYTIQEVKKLLAGEGGLVSYLGTSNGIEIDKRIKEGDQEAAFYF
                     QAMAYTVVKEIGAMYFVAKGKIDAVLITGGLANNETFVNHIKDYVTPIAPVFVYAGED
                     EMRALAEGALRVLNQEEKLQAY"
     gene            complement(7297..8214)
                     /locus_tag="PLP02_02445"
     CDS             complement(7297..8214)
                     /locus_tag="PLP02_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPF42344.1"
                     /translation="MLEKVEDLAKLAKKTAPIRMGVVFAHDPYVIEAVAKSREAGDIF
                     PVLIGNQEKIKEILREEALNMPCEIIDENDPDQASRIAMDLVNDGEIQVIMKGLLDTK
                     NLLKAVVNKESGIREKTLLSHVGLVSYPGFNRVLFVTDGAMNIDPGVEEKIMIIENAV
                     ELAHSLGYDNPAVGIVSAVEKVNPKIQSTVDASQIIKHYEENPPKGFVVDGPFAIDNL
                     VSEESVKHKGIVSMVAGHADILLFPNLDGGNIFYKTSVFLAGAHSAGIVLGAKVPIVL
                     TSRADTAMAKYNSILLAVVNHYGLRATDN"
     gene            complement(8207..8770)
                     /locus_tag="PLP02_02450"
     CDS             complement(8207..8770)
                     /locus_tag="PLP02_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPF42345.1"
                     /translation="MNETKNIKVAGFGGQGIMMFGQMIAYSATHDNVNSLWFPSYGPE
                     TRGGTANCSVIVSDKEINSPIFKDADHLVAFNAPSLEKFKDNVKKDGLVLYNSSMIPG
                     DPGCKKGLVVGVPINDIATDLGNMKVANMVMLGAYLELTHLFPDETIKQILIKFLGQK
                     KAHLLDINMMALASGKKYVADKGIKYA"
     gene            complement(8763..9512)
                     /locus_tag="PLP02_02455"
     CDS             complement(8763..9512)
                     /locus_tag="PLP02_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013173084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPF42346.1"
                     /translation="MRVKYQKSAGLTDAPLTYCPGCTHGIIHKLVAETMEELGIIDRT
                     IGIASVGCSVFSYEFFNCDMLQAPHGRACAQATGVKRVRPENIVFTYQGDGDLASIGI
                     AETIHAANRGENITVIFINNAIYGMTGGQMAPTTLIGQKTTTSQDGRVAAIHGNPIKI
                     SEIFAPIEGVAYLERVAAFDPKHVIKAKAAIKKAFQYQMENRGFTMIEVLATCPVNWG
                     KTPIDAMNWVKTEMTKVYPLGVFKDKGDNNE"
     gene            complement(9514..10581)
                     /locus_tag="PLP02_02460"
     CDS             complement(9514..10581)
                     /locus_tag="PLP02_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPF42347.1"
                     /translation="MAKVLMKGNEAMALAAIKGGAEAFFGYPITPQNEIPEYLSKHLL
                     ENGKVFVQAESEVSAINMVYGAASTGARVITSSSSPGIALKQEGISYLCGAELPAVIV
                     SVMRGGPGLGGIQPAQADYYQATRGGGNGDYHLIVFAPERIQEAIDILKQAFDIADCY
                     RNPVMILADGLIGQMMESVDFDRPVPHRDLPKKDWVASGMKNHKGRNYVSSIHLDPKQ
                     LEQHVFKLFEKYDLVKKNETRYEMINIENAEYVIVAYGTMSRICRSAIEILAKKGINV
                     GIIRPITIWPYPTEAFDKIPKTAKGILCAEMSMGQMLDDVRIANNGRLPVAFFGRTGG
                     MVPEPEELVDAILHFNERVVK"
     gene            complement(10583..10798)
                     /locus_tag="PLP02_02465"
     CDS             complement(10583..10798)
                     /locus_tag="PLP02_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPF42348.1"
                     /translation="MPKGRTTFDYEKCKGCLLCVEYCPQKILYQDKTTLNKAGYNIIK
                     VSDPDKCIGCGFCAIMCPDSVIKVEVE"
     gene            complement(10809..11456)
                     /locus_tag="PLP02_02470"
     CDS             complement(10809..11456)
                     /locus_tag="PLP02_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF42349.1"
                     /translation="MKRITFLTGYYGSGKTEIALNLAVKEKADYLVDLDIINPYFRSR
                     EAEEFLKGLGTKIISSDLKDSAYSDMPFLSKQIFLPFLQDGCRAIYDLGGNDLGAKVM
                     RQFEKIDKQIVDLFLVVNVYREETRDTTSILKLIDKIQGSSGFAITGIINNSNLLRET
                     TYDDIMSGEAKIAEASKISGIPLVYTCFYEELFQPSFAYMGEPLILKLYFRKKWY"
     gene            complement(11463..12533)
                     /locus_tag="PLP02_02475"
     CDS             complement(11463..12533)
                     /locus_tag="PLP02_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPF42350.1"
                     /translation="MNKFEYMEKHGYEQLIYFYDKTTGLKGITCIHNTTLGPALGGTR
                     LWNYASEEDAALDALRLARGMTYKAAAAGLNLGGGKTVLIGDASKVKSEAYFRALGRY
                     VQSLNGRYITAEDVNTSTKDMDYVHMETEYVTGLEEKSGNPSPITALGVYHGIRAALQ
                     QTFGNDDISKRTFAVQGAGQTGYFLIKMLVEAKAKKIYFTEINQHHIERMKKECPEVE
                     FVEPDKIYGLDVDVFAPCALGAILNDETIPAFRCKIIAGSANNALKDEDKHGMMIKDR
                     GILYAPDFVINAGGLINVYHELIGYCRERALRDTEKIYDRLLEIFSISNEQGISTYEA
                     AKVFAKNRIETVGKVRSNYVRR"
     gene            complement(12804..13772)
                     /locus_tag="PLP02_02480"
     CDS             complement(12804..13772)
                     /locus_tag="PLP02_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42351.1"
                     /translation="MDLAKLKSLYERNSLPENSLKEAIAFMETVAKIVDPDLADEAGI
                     KKIVSELVASGKNTVGNFVIMMRYFLATGNTDRYIQLTRYTGGLDVIENILKRLETRF
                     GKEKMDLAMQGLSIPILGTDPSDMAIFTARFMDNLKKNFNDKDIAFILDGNNHGVPES
                     AFISDKTLYEASSDLDEYLEKLHAQKVAVLQKHADLNKVWFEQKINQEVVDFVKANQE
                     ILSAVRKDDKLYMTKIPYDIDGYLHATDPKEKKYLGCHCPFAREAMKNGDTSISPLWC
                     HCSAGFEKLSFEFVLGTKLKIEILETILDGNERCRFAISLDGIKHK"
     gene            complement(13783..15339)
                     /gene="pruA"
                     /locus_tag="PLP02_02485"
     CDS             complement(13783..15339)
                     /gene="pruA"
                     /locus_tag="PLP02_02485"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPF42352.1"
                     /translation="MAIYEYRTEPLTDFSKEENRKKYEEALKKVGSELGGNYPLVIGG
                     RQIYTKNVYTSVNPANHSQKIGTICQAGIKEADEAMEAALKAFETWKKTDAKVRAGIL
                     FKLAAIMKRERFEFNAIMTLEAGKPWIEADADTAEAIDFLEYYGRQMLSLERIDDAVL
                     SRRDIEKNEYRYIPLGVGAIITPWNFPMAILTGMTSAAIVTGNTVLLKPAMTTQVIAY
                     KFVDALKRAGLPDGVVNYMPGRGSEIGDYIVKHPKTRFISFTGSKDVGQTIYENAAKV
                     NKGQIWLKRVIAEMGGKDAILVDSDCDTEMAAQAIVASAFGFSGQKCSACSRAIIHQD
                     VYDEIVTKIVEKTKKLKVGDPSDFQMNMGPVNDDKAYKMITDYIEIGKKEGKLVAGGK
                     YSDEHGWFIDPTVIVDLDPHARIMQEEIFGPVLAVTKVSSFEEGIMVANDTEFGLTGA
                     AISNNRAHLEMAKADFHVGNLYLNRKCTGAIVGYQPFGGFNMSGTDSKAGGPDYLVLH
                     MQGKSIAEAL"
     gene            complement(15356..16498)
                     /locus_tag="PLP02_02490"
     CDS             complement(15356..16498)
                     /locus_tag="PLP02_02490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPF42353.1"
                     /translation="MKTEILIIGAGISGTSIAYNLAKEGITDIVVVEKGFLTSGSTGR
                     CGAGVRQQWGTKANCIMAKHSIEFFENAEKTLDYDGDIEFKQEGYLILAVTPEEDKAF
                     SENVVLQNDLGIPSVKCNKEEALKIVPHLNPESFISATFCQTDGHLNPFKMTDAFYQK
                     AHKMGVNFLFSEEVESIIKTQDGFLVKTNKREIEAEKVVNAAGGYSHEIGMMVDLDIP
                     THSEKHEILVTEPVERMQGPMVMSFAKNIYCQQVPHGSFLIGRGDPKQKPSHDMDSSW
                     EFLDEMSKTVMEIMPKVGKLRVVRQWAGLYNLSPDRQPIISAVPEIPGFFVACGFSGH
                     GFMFAPITGLMMTQIITGKETMLDRADYDLHRFKINKTTVKEKSVV"
     gene            complement(16488..16769)
                     /locus_tag="PLP02_02495"
     CDS             complement(16488..16769)
                     /locus_tag="PLP02_02495"
                     /inference="COORDINATES: protein motif:HMM:NF037640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPF42354.1"
                     /translation="MNDKNVIICRCEDVSRQDVIDMMKKGFVTVEDLKRQLRIGMGPC
                     QGQNCCELLKQEIARFTGENPENIPMPRQRPLVMGVKLEKIAERAHDED"
     gene            complement(16762..17247)
                     /locus_tag="PLP02_02500"
     CDS             complement(16762..17247)
                     /locus_tag="PLP02_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPF42355.1"
                     /translation="MTLLKNGVPSQEQVRQKFPSEAVLARPKAILECYEEIPCNPCST
                     SCPFKAITIGEDINSIPQIDHDKCTGCGICISSCPGLAIIVAQTDSDKAIFKIPYEFI
                     PVPVPGEKWIGLDRSGNPICEALIVKADTKKTNDRTVVVTVETERRFLYDFITIRCPY
                     E"
     gene            complement(17244..18329)
                     /locus_tag="PLP02_02505"
     CDS             complement(17244..18329)
                     /locus_tag="PLP02_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF42356.1"
                     /translation="MTEKELMIIGAGPAGLSAAKVALEAGCGVTIVDRNKYPGGQLAK
                     QTHKFFGSEKQYAKTRGFDIAGILKSNLGKYHDKLDILTDTTAVGLYKDHVLTTLSNE
                     EYRKYHAQAYIMATGASEKFLAFENNDLPGIFGAGAVQTLMNLYGVLPGKEVVMVGSG
                     NIGLIVTYQLMQAGVTVKAILEAAPTIGGYKVHAAKIARLGVPIYVSTTIKKAIGDKE
                     LEKVVTVSLDESWKEIPGTEKEIYCDTLTIAVGLSPLHQLMSMAGVKMKYVKELGGLV
                     PLLDKRLETSMDNVFACGDVSGIEEASSAMAEGALAGLFAAENLGYRHPDFDKLVADF
                     EKQLQDLRQGPSGTKIRRGLERLGERS"
     gene            complement(18326..18631)
                     /locus_tag="PLP02_02510"
     CDS             complement(18326..18631)
                     /locus_tag="PLP02_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPF42357.1"
                     /translation="MRLKKHPILDVTEKRKIDFYFDGRPMSGKEGDTIAAALHASGIK
                     VLSKSIKDGRNRGLYCAIGNCGSCHMTVDGIPNVKTCITELKDGMVVESQKGLGRLE"
     gene            complement(18758..20182)
                     /locus_tag="PLP02_02515"
     CDS             complement(18758..20182)
                     /locus_tag="PLP02_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPF42358.1"
                     /translation="MITLYLNTDGKEPLYMQLYIQIRDAILEGKIYPQEKIPSKRKLA
                     EDLGVSVQTVENAYGQLTAEGYIYSKPKSGYYVEDVSVFFQYSKTDRVKQYEHIVIKK
                     KNKADVAYDFSTNVVDDIEFPYENWSKEFRNVFAERILFDINGSSPSGLEYLQTEIAG
                     YLYRYRGISANPRSIIIGSGTEYTIMLIRLLLGDNRKIGVENPGYPKLMRMYEAMGIS
                     TVPINLDENGIKIDMLTESEANLVHVTPSHQFPCGMVMPASRRSVLLKWAEIKNDRYI
                     IEDDYDSEFRFSGAPIRAMKSLDFNDRVIYVNSFAKSLFPGLRVNYMVFPDHLAKKLE
                     SDYSYITCPVPVFVQQFLADNINTGKFEQHLARMKKIYKSKRDTLIYALSQSRLTENF
                     GIKGEEAGLHFLLESQSEIDESKYMENARKAGIKIKGLREFCLKKIPLASTFVMGYSK
                     MEKTAMTSAVTALENVFCMLDPKM"
     gene            20290..21165
                     /gene="pdxS"
                     /locus_tag="PLP02_02520"
     CDS             20290..21165
                     /gene="pdxS"
                     /locus_tag="PLP02_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009230280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPF42359.1"
                     /translation="MEKTRYDLNKELAQMLKGGVIMDVTTPEQAKIAEASGACAVMAL
                     ERIPADIRKAGGVSRMSDPKLIKDIQAAVSIPVMAKVRIGHFVEAEILEALEIDYIDE
                     SEVLSPADDIYHIDKTAFKVPFVCGAKNLGEALRRISEGAAMIRTKGEPGTGDVIQAV
                     RHMRLIQAEIRRVVSLSEDEIYEAAKELGVSIDLLKDVKKTGKLPVVNFAAGGIATPA
                     DAALMMKLGAEGVFVGSGIFKSGNPAKRAEAIVKAVANFNDPAILALVSENLGEAMVG
                     TNEEEIALLMANRGE"
     gene            21170..21733
                     /gene="pdxT"
                     /locus_tag="PLP02_02525"
     CDS             21170..21733
                     /gene="pdxT"
                     /locus_tag="PLP02_02525"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPF42360.1"
                     /translation="MEIGILALQGAFIEHEQAFEDIGISTHEIRNRKDLEKPMDGLVI
                     PGGESTSMVKLLKGLSMWDKLKDMLDKGLPAFGTCAGLILLAKVNDGRQDSFGSIDIE
                     AKRNGYGRQIDSFFAYGETEDNTLFPLVFIRAPIITSIGPDVFKAATMGKNIVACRNN
                     NILVCAFHPELTENRYFHRLFVDICKR"
     gene            complement(21814..22926)
                     /gene="alr"
                     /locus_tag="PLP02_02530"
     CDS             complement(21814..22926)
                     /gene="alr"
                     /locus_tag="PLP02_02530"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPF42361.1"
                     /translation="MKQTEIQNRCVAEVNLETLAENYRILSERVGPKTEVMAVVKADA
                     YGHGAVKTACKLAEIGASHFGVATLEEAIELREAGIKGEILVFGLIDACDFSFLTEYG
                     ITPTMISVNQAKEYAKHKIRYKAHINLDTGMSRMGILCRTEAETETAFAEIMEIIRTG
                     FCITTGIYTHFAISENTENPFTDLQFSLFSKVLDKLDKAGVDIPLRHCCNSAATILHK
                     EMHLDMVRCGIALYGLPPVKTDLRLKPVLTLKARVVRVAKLKKGMPVSYGCRYVAKED
                     MEVATISIGYADGLSRLLSGIISVSKDGKEFPVIGTICMDLAMVGIEKGELKPGDFVE
                     IFGTGDINADTIAKKIGTISYEILTDISPRVKRKYI"
     gene            23154..23774
                     /locus_tag="PLP02_02535"
     CDS             23154..23774
                     /locus_tag="PLP02_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPF42362.1"
                     /translation="MIDKLNNGEVDATRMSQYADWVELLFEYSDQTILKTGTYDDQVQ
                     KFDSGHAAFIHQGNWIDPNLDEATFTMGYAPHASVYGTCDSIFIAAPSYYVINSDSEN
                     IDTAKLFLNDLASTTAGMDYTVNQAGMISAFKSCTLLPSGGLSQALVTWMNAGKAYSW
                     WQNDMPSNFGMGTLGPIYTLYANGTIDKTGFITQITAAIEALAPEE"
     gene            complement(23837..25738)
                     /locus_tag="PLP02_02540"
     CDS             complement(23837..25738)
                     /locus_tag="PLP02_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42363.1"
                     /translation="MPKPFNAGPREKFNIKKHGKTLKRLLGYIFRDYKWRFIFVLVCI
                     LFSAIAGAAGSIFIRILIDDYITPMIGVSDPVFSGLLTAIGILALIYVVWIASSYLYN
                     RTMVVMAQGIMKNIRDEMFSNMQDLPISYYDGHTHGDLMSRYTNDADTLRQMLAQSVP
                     QFISSMATVIVIFFAMLFSNAVLTLIVIVCVVLMMMITKKIASVSGKYFVAQQKSLGE
                     TNGFIEEMVEGEKVIKVFCHEEKTKEQFDVLNDKLRVNATMANKFATLMMPVMNNLGN
                     LQYVIIAVIGSALCILGFPGITLGVVASFMQLSKNFTMPINQIANQFNSIIVALAGAD
                     RIFQLMDAKKEEDEGYVTLVNVERQPDGTLKETKEKTNLWAWKHLHKDGKLEYVLVQG
                     KVTFNDVDFAYEPGKTVLHNITLQADKAEKIAFVGHTGAGKTTITNLLNRFYDIADGK
                     IRIDGININKIRKPDLRHSLGMVLQDTNLFTGTIKDNIRFGKLDASDEEIIDAAKRSN
                     ADGFIRMLPDGYDTMLTPGGNNLSQGQRQLLSIARAAVANPPILVLDEATSSIDTRTE
                     AIVQKGMDRLMEGRTVFVIAHRLSTIHNSDVIVVLDQGRIIEIGNHEKLINKKGMYYQ
                     LYTGAFELE"
     gene            complement(25731..27476)
                     /locus_tag="PLP02_02545"
     CDS             complement(25731..27476)
                     /locus_tag="PLP02_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42364.1"
                     /translation="MIRKLAKNIGKYKFDTFMSPLMISLEVVMEVIIPLLMAKLIDDG
                     ITPGDLSAVVKFGLLLVLSASISLGFGSLSCVFSANASAGFAANIRKKMFDNVQDFSF
                     RNIDKFQTSSIITRLTTDVSNVQNAFQMLIRIAFRAPIMLVAALIITISISPTLSLTF
                     LLVIPILIIGFIFIIKSVFPVFTEVFKKYDGMNNIVQEDIAGIRVVKTYVREDYEKEK
                     FRASSQEIYKYFSKGEKILAAMSPLMQFSMYVCILVISYFGAVLIVKGIGGNMITTGE
                     LTSLITYSGMILSSLMMVSMVIVMITISRASAIRITELLDEKSDITNPNNPVTNVNNG
                     DIIFDHVDFRYSENSERKVLDDINFKIKSGQTIGIIGSTGSSKSSLVQLIARLYDSTA
                     GSIYVGGNNVKDYDIEALRNSVAVVLQKNVLFSGSIKDNLRWGNENATEEEMIRACRM
                     ARADEFIREMPKGYDTHIEQGGTNVSGGQKQRLCIARALLKNPKILILDDSTSAVDTR
                     TDAEIRKMFKNELQGVTKIVITQRISSIEDADAIIVMEGGQLNQMGTHEDLLATNAIY
                     REVYESQSKAVMENA"
     gene            complement(27469..27927)
                     /locus_tag="PLP02_02550"
     CDS             complement(27469..27927)
                     /locus_tag="PLP02_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF42365.1"
                     /translation="MKKNDLLGCKIIFLSHLIKRNREEEISKSTEGLTFLHFRIVKFI
                     RHNDDLPIFQKDLEKEFNVRRSTMSGTLDLMESNALIERKAVSGDKRLKQICLTEKAI
                     EISHKDEIDLTQFERKLAKGIDDGKMADLMEMLDILIGNMENYNGETKND"
     gene            28110..29612
                     /locus_tag="PLP02_02555"
     CDS             28110..29612
                     /locus_tag="PLP02_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42366.1"
                     /translation="MKKKHIVVSPFQQYRQSLSSESNELLTDFFASFDEHVLLPEKGK
                     SEMRSDFEKAILYFDSLGVRLDKSLERLDTKNLGGFYSRPAIRWFPLDTAAKIYPLSM
                     RRNQMAVFRLSAYMKETVNPEILQIALSFTIKRFPCFSTTIKKGFFWHYLDTTKRRFV
                     VEKEYDIPCRPLDVSLSGSQTFRLLYHENRISVEIFHILADGYGGMVFLKTLVAEYLR
                     LQGIDITYDKTILPINETPVDGEMSDAFFFVEPKNKASGFLNRPSVQLSGSLSRTKPC
                     RVLHFIMDTDKLKAVAKSKNSTITSYLVANMMLAGKSATDEPSGSMSIQVPVDMRKYY
                     PSGTLRNFSLYCGIKLPLTKITDCESILPEISRQLVEKGALGPMNEMMNAAKGLVRSL
                     RFVPLFIKNPVARIIYGFLGDQAFSNTLSNLGIVNLPEEMASHVVGFDFLLGTSITNR
                     VSCSVVSYNNKTTFSIAKLTLDPSYEETMYKLLKSDGIDVIVEGSGIYGS"
     gene            29602..30174
                     /locus_tag="PLP02_02560"
     CDS             29602..30174
                     /locus_tag="PLP02_02560"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HPF42367.1"
                     /translation="MEVKITYPARDRKKSRALLLRSVVKWLFVASAYVCPIINISAGG
                     PAWSIYVLWFLLFVWSFAFSPDLVEYNLISQTAKFLVFTCVLLALIDIIHSLGWAMFV
                     IPIICFGGLAVIAVFFFSNINIQRQNIMPMMWVIIASIIGIVVAISGWKQMSWPMIVL
                     ISLSVAILLASAAVLGKGFIREIIKRFSIK"
     gene            complement(30240..30683)
                     /locus_tag="PLP02_02565"
     CDS             complement(30240..30683)
                     /locus_tag="PLP02_02565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPF42368.1"
                     /translation="MITTFKASVKKTEKGLQTEGTARGFKIILDEPAGLGGSDTGMNP
                     VEALLVALGGCQAICAAAFAKANGVKFEEFHTELEGDIDLDGFMGKSDVRPGFQEIRF
                     TMHFKTDEPQEKIDKFVKFIEKTCPVGDTIGIGTKLVCAGGVIDK"
     regulatory      31158..31235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      31293..31368
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            31414..31893
                     /locus_tag="PLP02_02570"
     CDS             31414..31893
                     /locus_tag="PLP02_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42369.1"
                     /translation="MKKVFVAILVALLLGGTALAFSWWDNLSREQNETLQIGEGVTLV
                     VTAVATAPADKVLVPAGVTMTADDVDQIQLTYNVNLDQTVIDPLTLSVVATNVLIGGS
                     STYADLINIDITQAATTVNDANVLVTVTVSLDMPSTQAIYDEIKNGQITFSLTFTGE"
     gene            31957..32553
                     /locus_tag="PLP02_02575"
     CDS             31957..32553
                     /locus_tag="PLP02_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42370.1"
                     /translation="MKKRNILLVVIIALLFGSSVAAYAYWDNLQQNTNGNYVVGYGVR
                     LEVPTVVQDERALVPAESFYAAYEADYTTSYAFTYTLSLEDDLQYGMEADLAVDITDF
                     QIAGIAELFNSEEGLFTVTVGTDEAAATTSSTGEWDFTDAFYYNHNTVEVTVTITLAD
                     NGSAGFDASDYERVAGKTTNFAIGFLLSNSNSSNQPAE"
     gene            32582..33232
                     /locus_tag="PLP02_02580"
     CDS             32582..33232
                     /locus_tag="PLP02_02580"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPF42371.1"
                     /translation="MAQEKSVPSNKKPKSSISKGIRFFLSVAIILGLGYVVFNYVPFI
                     AKYDYFVIATGSMEPVIMTHDVVIIDTSVDPDNLSVGDIIAFYTDINGDGTDEIVVHY
                     IYSISEDEGVRTFLTHPEVSEHQDPWTLYDEDIVGIHVLTIPKIGGFLLFAQSFFGRI
                     VLISDILILFLLLELVSDSNKTKKKPDDCQEQETLEKSTDSSSDETEDDSDIHSGN"
     gene            33280..33573
                     /locus_tag="PLP02_02585"
     CDS             33280..33573
                     /locus_tag="PLP02_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42372.1"
                     /translation="MKRKMLVVILTIAVFFMSFSSITYVGYSYFDSLSSNSQITLQIG
                     EWTMAPDEWDPTLKYDKGDQVTYNGVIYEAFRNVPANRAPDSFRGFFYWYIVS"
     gene            complement(33676..34479)
                     /locus_tag="PLP02_02590"
     CDS             complement(33676..34479)
                     /locus_tag="PLP02_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42373.1"
                     /translation="MSIGENIKQLRQENNLTQEELASKIYVTRNAISKWETDKGIPSI
                     DNLKQLATLFKVSLDLIVNEDDRIALAMVNTEKIRKFNDIISSVAIFFAYSLSGILIP
                     YIFFTLNRYDDPAIDYILLPLIYILIGLISVLREISWKYLLIGSALAMIPVYMFYDIL
                     LPESTLGLIGLGHYVLFVTAYFLMDLCIRFLQNRFDSAILKKVFMYTAILITGAYVIH
                     TIIASIYLYNCFVCSAPWYLEVVINSLLYAVPIAIPVILYFHFSQISAK"
     gene            complement(34686..35300)
                     /locus_tag="PLP02_02595"
     CDS             complement(34686..35300)
                     /locus_tag="PLP02_02595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPF42374.1"
                     /translation="MSTKWIFFDLGSTLIDETKAYIHRIREMTEGTKVSYDDFENKRI
                     EFAGKGLDDIQETIKYFRLKKTPWHLEDEILFMDSVETLDYLKNRGYKLGIIANQMPG
                     VSQRLDRWGILKFFDIVVSSAELGVSKPDKRIFEKAFELAGCLPTESVMVGDRLDNDI
                     KPAKSLGMKTIWIRTELSKYQDSNYGKDIADKTIDYISKLQDIF"
     gene            35467..>36180
                     /locus_tag="PLP02_02600"
     CDS             35467..>36180
                     /locus_tag="PLP02_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42375.1"
                     /translation="MKKLSKAVFLLLLSLYSVFFAEIIAGSSNYPLFDLWGLFVVIPL
                     YGFHVIILLTIIIRFQKGKVIPFRTMYFAGMLFALYEAYITKVLWIGFSDDPHIFLGI
                     GLIEYLILVPFWHPIFSFIIPSALLERTLLKSSYIFDGLPNFMKHILNRKAGIISLFA
                     IIGVFAAINGTFPILMLSMISMLVPMLLVIYLLKRRHLTNEYTLKDIMPRKQTFIIMC
                     ILLLGLYVFGTLWIRFDAIT"
BASE COUNT         8940 a         8528 c         7804 g        10908 t
ORIGIN      
        1 tggctgttcc agccgttttc agtgccttgc tcatgctcgg agtaaatcgc aacgccgaac
       61 gcatggtagc tgaaatccat cgccaattta aagaaatacc cggtcttaaa gaaggtaaag
      121 aaggcgtcag tcctgaatcg gacaaatgtg tcgccattgc caccaaaggc gctttgaaag
      181 agttgattcc cgccggtctt ttcgctatca taatgacttt ggccgtcggt ttcatcggcg
      241 gcgtcaacgc catcggcggt ttcttggccg gaaacatcgt gagcggatta ctgctcgctc
      301 tctttatgtc caacagcggc ggtttatggg ataatggcaa gaaatacatc gaagccggag
      361 ccaacggagg caaaggcagt gatgctcata aagccgcagt ggtcggcgat accgtaggcg
      421 atcctttcaa ggataccgcc ggtccttccc taaacactca aatcaccgtc gtcagcctgg
      481 tcgccagtct gatgagcgtc atcttcctga caatgtccat tttctgattc ataaaatcaa
      541 aaaaagaact ccggatattt ccggagtttt tatttcaata attttctggc aatattgcct
      601 tttgcttcgc tatacggatg atatcgcatt tccaagtcaa ccagattgct tctttcgagc
      661 acgcttctca tgttggaaaa agtctcaaaa ctgaatttcc cgtgatatct tcccatgccg
      721 ctggcaccga ccccgccgaa tcccagttcc gtggaaccga agtgcataag agtgtcgttg
      781 atcgtagcgc cgccgaaact gatcctgtcc agggcttctt ttctgacttt acggtcattt
      841 gtgaataaat agaacgcgag aggcttttcg tgatcgatga tcatgtcgta cgcttggtct
      901 agactatcaa aatttataat cggaaggact ggcccgaaaa tttcttcttt catgatgtca
      961 tcttcccagg taacgcctgt catgacggtc ggcgcgatct tttcgttcgc atgctctcct
     1021 cccaaaacga ttttctttgg attgatgagg ccaagcagtc tttcataatg tttctgattg
     1081 attattttcg ggagttcctc acaaagaagc ggatcagaac cgaaaaacaa tctgatgtat
     1141 tttttcaatc cttcgaccag atcattttca tcctctttcc ggatcaaaac ataatccggt
     1201 gcaacgcagg tctgtccggc attgatgaat ttcccgaacg caagtctctt ggcaaccaga
     1261 tccatattga cgcctgaatc gatcagacat ggactttttc cgcctagttc caacgaaaca
     1321 ggagtcagat acctgcttgc tttttccatc accattttac cgatattcgt gctgccggta
     1381 aaaaagatgt agtcgaacct ggtttccaga agttgttggt tttcttctct tccgccgaga
     1441 actaccgcaa catattccgg tttgaaagac tcttcgatga ttgttttgat cattttgcag
     1501 gtttccacgg catatgagcc cggtttgaca acagccgtat tccccgccgc caaggctccg
     1561 gcaagcggtg ttaaggccag ttgcaaagga tagttccacg gactcatgat aagaacggtc
     1621 ccaaaaggtt ccggcataat atagcttttg gctttgaaaa gaacgatcgg agttctgact
     1681 tttttgggcc tggccaattt tctcagatgc acaataaagt agtcgatctc gctcaatacc
     1741 aacccgattt ccgttatata agtttcgaaa ggggctttcc cgagatcgtt tcggagcgct
     1801 ttctcgattt cgccctcatg cgatacgatc gcttctttga gcttttccaa agccgccaag
     1861 cggaattcca cgtttttggt ggctcccgtc cggaaaaatt ctctctgttt ttctaccaat
     1921 ggtttgaaat ccattctgta cacctccata tgccgaaaca gtaacattca ttatatcaca
     1981 atcaagtgtt ttttggtata atatattcat ccgaaacatt cgaaaggaat ccattcatga
     2041 tctatcttac aggcgccacc ggacatctgg gaaacaatat cgccagatat cttcaaaacg
     2101 aaggcattcc atttacaatt ctattgagaa aaaacgggaa ggctctactt gatttgaatg
     2161 atgtaaagta cgttttcgga gatattttcg atccggtttt tctttccaat aacatttcgg
     2221 aaaacgacac gctgatccat tccgccggct tcatagacct taataaccac cacaagaaag
     2281 aatgcgactc agccaactat cttggtaccg agcaaatcat cgatttttgc cagaaaagca
     2341 aggtccggct catctatata agtaccgtcg attgcatacc gagggaaaaa tcacagggtt
     2401 tcatatccga accggaatcg cttgatgtgg acaatgaagg ctctaattat actgtttcca
     2461 agggtaaagc tactgcttat cttcttgaga aaatgaaaaa cgagaaaatg cctgccgtta
     2521 tcctatatcc ttccgcaatc atcggtatca acgatttcaa gccgtcggca ctcggtaaag
     2581 agatccgcaa agcgatgcgt cgccggatat ctttctattt ccggggcgga tataatttca
     2641 ttgatgtccg cgactgcgcc aaggccgtcg tagaatgtgt caagaacaat cagtccggct
     2701 gttatatcct ggccgccaac aaccgaagcg tcaaacagct tctgaaggaa gtcggcaaag
     2761 tccaaaacca caaggttttc ttgattggag taccggtttt tcttgtcaga acattctcgt
     2821 ttctaattcc ggacatctcc gatttcatga tcgatacgat gaccgacaat tacaattatg
     2881 acaattcgct catgcttcgc gatctggttc cggaaccgtt cacgttcgaa aaaacggtgg
     2941 acgacactgt caaatggttt tccgaaaacg agcatatttt cgagtaatga caaaaatatc
     3001 ttctggtttt gtttcagatg gctttgtaaa tatgcaatgt ggatcttatt caattagtca
     3061 taaaaaagaa agttgtcccg atggacaact ttttttgatc tattttttcg attccgtata
     3121 tttgtcttcg accgctttga cgatcgaggc aaattcttcc ttttcttcca tgtcttcgat
     3181 cgaaagccaa aggatcttct tttcgggtat tttgtaagct ttgaatattc ttttcaaaac
     3241 atcatagtaa tttgcgtaat ggaaagccag atattcctgc tggtcctttt cgattacgcg
     3301 gcctcttctc cttacgtttt cgaaaagttg ttcggtcctt ctcatgatga ctatgtggac
     3361 atcgacatcc gggatccttt tcatatgttc gtctgctatc gcataagtca gcgctacttc
     3421 catcgccgac atgattcctt ccagccttag gagttccgta aataccaggt tggaaaaaag
     3481 cgttgaatcg caaacgagat tgtcaatatc cttcgatttc cacatcaaca ggaatagtgc
     3541 gctgtaaaat gcgttttgcg acagaagggc gaatgtttcc ttatcttggt agaacaaagg
     3601 aaggaattca tttttttcca ccggttcctc gaccagttcc gcgttgtaat atcttgcaag
     3661 ttttccggcc aaagtggatt tgccgctgcc tatcgggccg attattccta ttctcatgtg
     3721 gacctccgtg agtaaattca aatatgtctt cattatactg aacatatttt tgtttgtcaa
     3781 agacagaacg ccttttattg caaaaatatt ttcaggaatg ttttcctgtt tctttttttc
     3841 gttatatact tattaatttg caggtttttc tttgcatgaa tatctttata taatatattt
     3901 ttgcaatttg gtttttagta tatatgcaac ttttaatagt tgcacaaata tgtgtccatt
     3961 tatgatggat atttcattat ttatacataa attttccaaa tcggattccg aatataataa
     4021 ataatcaagt ttaaacaaaa ttctgaataa tgtcttgtcg gcactttggc aagtttcttg
     4081 tttaccattt tgatatatat cattcttata agttttagag tatatttaca tacaacaaac
     4141 aaaaagcgcg gaagatccgc gctttctaac tatatttatg cttttttcag tacatttctg
     4201 gtgccggtgc caccggtttt tcttccttga tctccgctac gccgacttcc gttgtgatga
     4261 agagtgcgga tatcgaagca gcattcatga tcgcgcttct ggtaactttg gtcggatcga
     4321 cgattccttc tttgaacatg tcgacccaaa cgccgttttt ggcatcgaat ccgtaattct
     4381 tttcagcctt tttctgcaat tcgacgattt ccaaaggatc atagccggaa ttctcggcga
     4441 tctggtatat cggtgctagc aggctttcaa caaccacatt gatacctttt tgggtatcat
     4501 tgacatttga cttgagattt gccttgattt ctttataaat ttcgacaaga gcggctccgc
     4561 cgccgataac gataccttcc tcgacggcgg ccttggtcgc attgagagca tcttcgatct
     4621 tcattttctt ttccttgagt tcggtctcgg ttgttgcgcc gactttgacg atggcgacgc
     4681 cgctagtcag tttggccaaa cgttcgccaa gacgcttttt gtcataatcg gaagttgtcc
     4741 ggtccatctg tttcttgatt tcgtcgactc tggcggtgat tttcttcttt tcgccggagc
     4801 cgtcgatgat tgtcgtattg tctttgccaa tgatgacttt tttggccaga ccaagatcgt
     4861 ctatcttgac atccttgatt tcctgattca aatctttggc cacgaaacgt gccccggtca
     4921 gggtggcgat atcggtaagg atttccttct ggttgtcgcc gaagcccgga gctttggtgg
     4981 cgaccacatt gaaagttccg cgcagtttgt tcacgatgag ggtggaaaga acttcgttct
     5041 cgatgtcgtc tgcgattatg agcaacggtt tgttggcctg aacgatttct tcaagcaaag
     5101 gaagaatatc cttgatagtc gtgatcttct gatcggtgac cagcacattg gcattttcca
     5161 tgacaatttg cattttttca cggtctgtga ccatataggg ggaaatatag cctttttcgt
     5221 attgcaagcc ttcgactact tccaaataag tatcgaatcc tttggactca tcgacgctga
     5281 tgacgccgtt gtgaccaacc tttcccattg ctttggcaat gatgtcgccg atttcggaag
     5341 atcccgatga aacagtagcg acatgggcga tatcttcatc ggtttcgatt ttatgcgatt
     5401 tttccaacag tttcttcgcc acttctttgg cggctttttc catgccttcc cgcatcagga
     5461 ccggattagt tcccttttcg acatgttcga ttcccttgtg gatcatcgac tgggtcaaca
     5521 cggtggcggt agtggttccg tcgccggcta cgtcgtttgt cttgttggcg gcttcataaa
     5581 caagtttggc gcccatgttc tcgaaagcgt ccttcagttc gatctctttg gcgatcgtta
     5641 caccgtcatt cgtgatcatc ggggacccat atcccttgtc cagcacgaca tttcttcctt
     5701 ttgggccaag ggttactttg actgcatcgg ccaaaatgtc gacgcctttc aacatgccca
     5761 ttctggcttc ttttgaataa cgtacttctt tgctcatctc aatcactcct cttccaggac
     5821 cgcgagaatg tctttttcgg cgatgatcag atattcttcg tcatcgtact tgaattccgt
     5881 ggtcgaatat ttcttgtata caaccttgtc tcccgttttg acggtcagtg gtatcagttt
     5941 gccgtcgacc aggttccctt ctccgacagc tacgacatcg gcgaaactcg gttgttcttt
     6001 gacatcaccg gtcaggataa ttccgctggc tgtctttttt tctacttttt cttttttcag
     6061 tactacgttg tcatgcaatg gtttaagcat atcattcact ccctgtttca gttattagca
     6121 ctcctatttt ccaagtgcta gacatattat atcatttttc ttggcactgt caagtattga
     6181 gtgccagcaa attatctgtt tttgtatttt ttttatatga aaagggcttc cctgtcggga
     6241 agccgctggt ttcaatatgc ctgtaatttt tcttcctggt tcaagaccct gagagcacct
     6301 tccgccaagg cccgcatctc gtcttctccg gcataaacga ataccggggc aatcggcgtg
     6361 acatagtcct tgatgtggtt cacgaaggtc tcgttgttgg cgagcccgcc agtgatcagc
     6421 acggcgtcga tttttccttt ggcgacaaaa tacatggcgc cgatttcctt gacaaccgta
     6481 taggccatcg cctggaaata gaatgcggct tcctggtcgc cttccttgat ccttttgtcg
     6541 atttcgatac cgttcgaagt tcccagatat gaaaccaacc cgccttcgcc ggctaaaagt
     6601 ttcttcactt cctgaatcgt gtactttccg gagaagcata ggtccacgag cggatatgtg
     6661 ggaactgttc ccgttctttc ggttgaaaac ggtccgtcgc cgcccagggc gttgttcacg
     6721 tccacgaccc ggcctttctc atgcaatccg acgctgatgc cgctacctag atggcagaca
     6781 atcaggttca tgctttcata cggttttccg attgttttgg catgtctttt ggcaatcgcc
     6841 ttgtggttca gaacatggaa cacacttctt ctttcgattc ctttaagtcc cgatactctg
     6901 gcgaaatcgt tgaattcatc gaccgacacc ggattgacgg tgtaagccgg acggttgatc
     6961 ggcttggcca gttcgtaggc gatgatcgca cccaaattgg ccgcatggct tccgtaccga
     7021 caatttctga gatcgtcgac cattttttcg gtcacaagat atgtcccgcc ctgtttcaga
     7081 ggccgtaaca acccgcctct tcccacaaag atgtcgatct gctccaccga gtaatgatgt
     7141 tcggccagga agtcaaggat cagttgtttc cggaattcgg cctgatccat cgctgtcgca
     7201 aattctttca gtttctcgac atcgtgtttc aagacctgtt cctgaatcaa tttgtcattg
     7261 atgaagatgg cgaccttggt cgaaacgccg ccggtgttaa ttatcagttg cacgtaatcc
     7321 ataatgattc accaccgcta atagaattga attatacttg gccatggccg tatcggccct
     7381 gctggtcaga acaatcggaa ccttagcacc gagaacgatg ccggccgaat gcgcgccggc
     7441 cagaaaaacc gatgttttgt aaaaaatgtt tcctccgtcc agattcggga aaagcaatat
     7501 gtcggcgtga ccggcaacca tgcttacgat tcccttgtgt ttgaccgatt cttcgcttac
     7561 gagattgtcg attgcgaacg gtccgtccac aacgaaacct ttgggaggat tttcttcgta
     7621 gtgcttgata atctggcttg catcgacggt cgactgaatt ttcggattga ctttctccac
     7681 cgctgacacg atgcctacgg ccggattgtc ataacccaga ctatgggcaa gttccaccgc
     7741 gttttcgatt atcatgattt tttcttcaac gccgggatcg atgttcattg ctccgtccgt
     7801 gacaaaaagc acgcggttaa aaccgggata ggacactagg ccgacatggc ttaagagtgt
     7861 cttttccctg attcccgatt ccttgttcac gacggctttc agaaggttct ttgtatcaag
     7921 cagacctttc atgatgactt gaatttcgcc atcatttacg agatccatcg cgattctcga
     7981 agcctgatcg ggatcgtttt cgtcaatgat ctcgcatggc atgttcaagg cttcttcccg
     8041 gagtatttcc ttgatttttt cttgattccc gatcaaaaca ggaaagatat cgccggcctc
     8101 acggctttta gccacggcct cgatcacgta cggatcatgg gcaaaaacca cgcccattct
     8161 gatcggagcg gtctttttgg ctagtttagc gaggtcttcg actttttcaa gcatacttga
     8221 tccccttgtc ggcaacatac tttttgccgg aagccaatgc catcatgttt atatcgagaa
     8281 gatgagcttt tttctgaccg agaaatttga tcagtatctg tttgatcgtc tcgtcgggaa
     8341 acaggtgcgt aagttcaagg taagctccaa gcataaccat gttcgcaact ttcatgtttc
     8401 cgagatcggt tgcgatatcg ttgatcggca cgccgaccac gaggcccttc ttacaaccgg
     8461 gatctcccgg tatcatcgaa gagttgtaaa gcacgagtcc gtcttttttg acgttgtcct
     8521 tgaatttttc cagcgacggg gcattgaatg cgaccaaatg gtcggcatct ttgaatattg
     8581 gtgaattgat ttctttgtcg gaaacgatca ccgaacagtt ggcggtacca cctcttgtct
     8641 ccggtccgta ggacggaaac caaaggctgt tcacgttgtc atgagtggcg ctgtaagcga
     8701 tcatctggcc gaacatcatg attccttggc cgccgaaacc ggcgactttg atgtttttcg
     8761 tctcattcat tgttatcacc tttgtccttg aagactccta acggatagac tttcgtcatt
     8821 tccgtcttga cccagttcat ggcgtcgatc ggggtctttc cccagttgac cggacaggtt
     8881 gccagcactt cgatcatcgt gaatccccgg ttttccatct gatactggaa agcttttttg
     8941 atcgccgctt tggctttgat tacatgtttt ggatcgaatg ccgcgactct ttccaagtag
     9001 gctactcctt cgatcggagc gaagatttcg gaaatcttga tcggattgcc atgaatggcc
     9061 gcaactcttc cgtcctggga agtcgtcgtt ttctgtccga tcagagtggt cggggccatt
     9121 tggccgccgg tcattccgta aatggcgtta ttgatgaaaa tgacggtaat gttctcaccg
     9181 cggttggcgg cgtgaatggt ttcggcgata ccgatggaag ccaagtcccc gtcgccttgg
     9241 taagtgaata cgatattttc cggtctgacg cgcttgacgc cggtggcctg ggcgcaagcc
     9301 cgtccgtgcg gtgcctgaag catgtcgcaa ttgaagaatt catatgagaa aaccgagcat
     9361 ccgacgctgg ctattccgat tgtgcgatcg ataattccca actcttccat ggtttccgct
     9421 accagtttgt ggatgatccc gtgggtgcat ccggggcagt acgtaagcgg agcgtcagtc
     9481 agaccggctg acttttgata ttttactctc atgttatttc accaccctct cattgaaatg
     9541 aagaatggcg tccaccaatt cctccggttc gggaaccatg ccgccggtcc ggccgaaaaa
     9601 cgctaccggc agacggccgt tgttggcgat tctgacatcg tccagcattt gtcccatact
     9661 catttcagcg caaaggattc ctttcgccgt ctttggtatt ttgtcaaagg cttctgtcgg
     9721 atacggccag atcgtgatcg ggcggataat accgacattg atgcctttct ttgccaatat
     9781 ctcgatggcg gaacgacaga tccgcgacat cgtcccgtaa gcgacgatga catattcggc
     9841 attttcaatg ttgatcattt catatcttgt ttcgtttttc ttgacaagat cgtatttttc
     9901 gaacagtttg aacacatgtt gctccaactg tttcggatca aggtgaatcg aagagacata
     9961 atttctacct ttgtggttct tcattcccga agcgacccaa tctttcttag gtaagtcgcg
    10021 atggggtacc ggacggtcga aatcgaccga ttccatcatc tgtccgatga gtccgtcggc
    10081 taaaatcata accggattcc ggtaacaatc ggcaatgtcg aaagcctgtt taagaatatc
    10141 gatggcttcc tggattcttt cgggagcgaa cacgatgaga tgatagtctc cgtttccgcc
    10201 gccgcgggtt gcctgataat aatcggcctg cgccggctgg atgccgccga gtccggggcc
    10261 tccgcgcatg actgatacga taactgccgg cagttcggct ccgcacaaat aggaaatgcc
    10321 ttcctgtttc aaggcgatgc cgggagatga cgaagatgtt atgaccctcg ctccggtgga
    10381 tgcggcgccg taaaccatgt tgatggcaga tacttccgat tcggcctgaa cgaatacttt
    10441 accgttttca agcaagtgtt tcgaaagata ttccggaatt tcgttttgcg gggtgatcgg
    10501 atagccgaaa aacgcttcgg ctcctccctt aatggcggcg agagccatgg cctcgttgcc
    10561 tttcatcaat actttggcca ttttactcca cctccacctt gataaccgaa tccgggcaca
    10621 tgattgcgca gaatccgcaa ccgatgcatt tgtccgggtc gctgactttg atgatgttat
    10681 aacccgcttt gttcagagtt gtcttgtcct gataaagtat cttttgcgga caatactcga
    10741 cgcatagcag gcaacctttg catttttcgt aatcgaacgt tgttctacct tttggcattt
    10801 tttcctcctt agtaccattt tttgcgaaaa tagagtttta atatcagcgg ttcgcccata
    10861 taagcgaagg agggctggaa taattcttca taaaaacagg tgtacactaa cggaatcccc
    10921 gatattttcg atgcttccgc aattttggcc tcgcctgaca tgatgtcgtc gtaagtggtc
    10981 tcgcgaagca aattggaatt gttgatgatt ccggtaatcg caaatccgga agatccctgt
    11041 atcttatcga tcagtttcaa tatcgaagtc gtgtcccttg tctcttcccg gtacacgttc
    11101 acaacaagga aaaggtcgac aatctgtttg tcgatctttt caaactgtcg catcaccttc
    11161 gctccgaggt cgttaccgcc gagatcgtag atcgcgcggc agccatcctg caaaaaaggc
    11221 aaaaaaattt gttttgacag aaaaggcata tcggagtagg cgctgtcctt gagatcagag
    11281 gaaattatct tcgtccccaa gcccttcagg aattcctcag cctcacgaga ccggaaatac
    11341 ggatttatga tgtccaggtc gacaagatag tcggcttttt ccttgaccgc cagattgaga
    11401 gcgatttcgg tttttcccga cccgtagtat cccgtaagaa aggtaatcct tttcattatt
    11461 tgctaccgac gtacgtaatt cgatcttact ttgccaaccg tttcgatgcg gtttttggcg
    11521 aacacctttg ctgcttcgta tgtggaaatt ccttgttcgt ttgagatgct gaagatttcc
    11581 aggaggcgat cataaatctt ttccgtgtcg cgaagagctc tttcacggca atagccgatg
    11641 agttcatggt atacattgat gagtccgccg gcattgatga cgaaatcagg ggcgtaaagg
    11701 ataccacggt ccttgatcat cattccatgt ttgtcttcgt ctttcagggc attgttggcc
    11761 gatccggcga taattttgca tctgaaagcc ggaatcgttt cgtcattcag gatggctcca
    11821 agagcgcaag gcgcgaaaac atccacgtca agtccataga tcttgtccgg ttcgacgaat
    11881 tcgacttccg ggcattcctt tttcattctt tcgatatgat gctggttgat ttcggtgaaa
    11941 tatatcttct tcgctttggc ttcgaccaac atcttgatca ggaagtaacc ggtctgtccg
    12001 gcgccttgga ctgcgaatgt gcgtttgctg atgtcgtcat ttccgaatgt ctgttgcaaa
    12061 gcggcgcgga ttccgtgata aacgccgaga gcagtaatcg gtgacggatt gcctgatttt
    12121 tcttcaagtc cggtgacata ttcggtttcc atatggacgt aatccatgtc ttttgtcgaa
    12181 gtgttgacat cttcggcagt gatgtatctt ccgttgaggc tttggacata gcggcccaga
    12241 gcccggaaat atgcttcgct cttgaccttg ctcgcatcgc cgatcaagac ggtttttcca
    12301 ccgccgagat taagtccggc cgccgcggct ttgtaggtca tgcctcttgc aaggcgcaag
    12361 gcatcgagag ctgcgtcttc ttccgacgca tagttccaca gtctagttcc gccgagggct
    12421 ggtcccaagg tggtgttgtg gatgcaggtg atgcctttga gtccggtggt cttgtcatag
    12481 aagtaaatca gttgttcgta accatgtttc tccatgtact caaatttgtt cataaaatcc
    12541 tccttgttat atgttgtttt ccattttatg gaaaacgctt tcatcatttt ttattataac
    12601 actaagaatc gaaaaattaa aggaaaaaag atcggctaac gaaaaaaatc cggttgcctg
    12661 ttaagatatg gcaaccgggt atgtgtgttt atgcggaaaa aaagcggcgg taatgccgta
    12721 aataaagcat agagtgtcca aatcccgttt tttggttatg gaacttttga aaacagtttt
    12781 tgccattttt tctccttctt ttgttatttg tgctttatcc cgtcaagact gatcgcaaag
    12841 cggcatcttt cgttcccgtc aagaatcgtt tccagaattt cgattttaag ctttgttccc
    12901 aaaacaaatt cgaatgacag cttctcaaat ccggcgctgc aatggcacca aagcggggaa
    12961 atcgaggtat cgccgttttt catggcttct ctggcgaaag ggcaatgaca tccgagatat
    13021 tttttttctt ttggatcggt tgcatgaaga tacccgtcga tgtcgtaagg gattttcgtc
    13081 atatatagtt tgtcgtcttt tctgacggca gaaaggattt cctggttcgc cttgacaaag
    13141 tcgaccactt cctggttgat tttctgttcg aaccatactt tgttcagatc ggcatgcttc
    13201 tgcaaaacag caactttctg tgcatgaagt ttttcaagat attcatcgag gtcgctagaa
    13261 gcctcgtata acgtcttgtc ggaaatgaag gcggattcag ggactccatg gttgtttcca
    13321 tccaaaataa atgcgatgtc cttgtcgttg aaattctttt tgagattgtc catgaaccga
    13381 gcggtgaaaa tcgccatgtc cgatggatcg gtaccaagaa tcggaatcga caaaccctgc
    13441 atggcaagat ccattttttc cttgccaaac cgagtttcca gtcttttgag aatgttctcg
    13501 atgacatcga gaccgccggt gtatcttgtc agttggatgt atctgtccgt gtttccggtc
    13561 gcaagaaaat aacgcatcat gatgacgaaa tttcccactg tgttctttcc ggatgccaca
    13621 agttcggaaa cgattttctt gattccggcc tcatcggcca aatcgggatc aactattttc
    13681 gcaaccgttt ccatgaaagc aatcgcttcc ttcaggctgt tttccggaag actgttcctt
    13741 tcgtacaggc ttttcagctt agcaagatcc atcgtcttgt tcctacaatg cttccgctat
    13801 cgatttgccc tgcatgtgca agacgagata gtctggcccg ccggctttgg aatcggtacc
    13861 gctcatattg aaacctccga acggctggta gccgacgatg gctccggtgc atttacgatt
    13921 aagatacaga ttaccgacat gaaaatcggc tttcgccatt tcgagatggg cacggttatt
    13981 ggagatcgcc gctccggtca aaccgaattc ggtgtcgttg gcaaccatga tcccttcctc
    14041 aaaactcgaa accttagtga cggccagtac aggaccgaat atttcttcct gcataattct
    14101 cgcatgcgga tcaagatcaa cgatgacagt cggatcgatg aaccagccat gttcgtcgct
    14161 atattttccc ccggcgacca atttgccttc ctttttcccg atttcgatgt aatcggtgat
    14221 cattttataa gccttatcat cgtttaccgg gcccatgttc atttggaaat cggacggatc
    14281 gccgactttc agttttttag tcttctcaac tatcttggtt acgatttcgt catagacatc
    14341 ctggtggatg atggctcttg agcaggcact gcacttttgg ccagaaaagc caaaggccga
    14401 agcgacgatc gcctgggcgg ccatctcggt gtcacaatcc gaatcgacaa gaatggcgtc
    14461 tttcccgccc atctcggcga tcacgcgttt aagccatatc tggcctttgt tgacttttgc
    14521 ggcattttca tagatagttt gtccgacatc tttggatccg gtaaatgaaa tgaatctcgt
    14581 cttcggatgc ttgactatgt aatcgccaat ctcggatccc ctgcccggca tgtagttgac
    14641 gactccatcc ggcagtcccg cccgtttcag ggcgtccacg aatttgtacg ctattacctg
    14701 ggtcgtcatc gccggtttca gaagcacggt gtttccagtg acgatcgcgg ctgaagtcat
    14761 gccggtaaga atcgccattg ggaaattcca tggcgtaatg atcgctccga cgccaagagg
    14821 aatataacga tactcgtttt tctcaatatc cctacgggat agaacagcat catcaattct
    14881 ttcgagcgaa agcatttgtc tgccgtaata ttcaagaaaa tcgatggctt ccgccgtatc
    14941 ggcatcggct tcgatccacg gtttgccggc ttccagggtc atgatggcat taaactcgaa
    15001 gcgttccctt ttcataatgg cagccagttt gaaaagaatc ccggcgcgaa ctttcgcatc
    15061 ggtttttttc caagtttcga aggccttcag agcggcctcc atggcttcgt cggcttcctt
    15121 gatgccggct tggcaaatcg taccgatctt ctgggaatgg tttgccggat tgaccgaagt
    15181 ataaacattc ttcgtgtaaa tctggcgccc gcctatcaca aggggataat tgccacccag
    15241 ttcgcttccg actttcttaa gcgcttcttc gtatttctta cggttttctt ctttggaaaa
    15301 atctgtcaac ggttctgtac gatactcata aatagccata tcaattcacc ctttctcaga
    15361 ccacggattt ttctttgacc gtggttttat ttatcttgaa tctatgcagg tcataatctg
    15421 ccctgtctag cattgtttct ttgccggtga taatctgtgt catcatcaaa ccggtgatgg
    15481 gagcgaacat gaaaccgtgt ccgctgaatc cgcaagcaac gaaaaatccc ggaatttccg
    15541 gaacggcact gatgatcggt tgacgatcgg gtgacagatt gtataacccg gcccattgtc
    15601 tcacaacccg gagtttgccg accttgggca tgatttccat cacggtcttt gacatttcgt
    15661 cgagaaattc ccatgaagaa tccatatcat ggctcggttt ttgcttggga tctcctcttc
    15721 caatcaggaa gcttccgtgc ggaacttgct ggcaatagat atttttggca aacgacatta
    15781 ccatcggccc ctgcattctc tcgaccggtt ccgtcacgag tatctcgtgc ttttcagaat
    15841 gagtaggaat gtctagatcc accatcattc cgatctcgtg cgagtaaccg ccggcggcgt
    15901 ttaccacttt ttcggcttca atctcgcgct tattggtttt aaccaaaaat ccgtcttgag
    15961 tttttatgat cgattcaact tcctccgaaa acaggaaatt gactcccatc ttatgggctt
    16021 tttgatagaa ggcatccgtc attttgaacg gattaagatg tccgtccgtc tgacagaaag
    16081 tagcgcttat gaaactttcc ggattaagat gggggacaat cttcaaggct tcttccttgt
    16141 tgcatttgac ggaaggaatt cccagatcgt tctgcaatac cacattttcc gagaaagcct
    16201 tgtcttcttc tggcgtcacc gcaagaataa gatatccttc ctgtttgaat tcgatatctc
    16261 cgtcgtaatc aagtgttttc tcggcatttt caaagaactc gattgaatgt ttcgccatga
    16321 tgcaattggc cttcgtgccc cattgctggc gcacaccggc tccgcatctt cccgtcgatc
    16381 cgctggtaag aaaacctttt tcgacgacta cgatatcagt aattccttct ttggcaagat
    16441 tataggcgat gctggtgccg ctgattccgg ctccgattat caatatctca gtcttcatcg
    16501 tgagcacgct ccgctatttt ttccagtttg acgcccatga caagcggacg ttgtctcggc
    16561 attggtatat tttcgggatt ttccccggtg aatctggcaa tttcctgttt caacagttcg
    16621 caacaattct gcccctgaca tggacccatg ccgattctca attgtctttt caagtcttcg
    16681 acggtcacaa aacctttttt catcatgtcg attacatcct gcctggaaac atcctcacaa
    16741 cggcagatta tcacattttt atcattcata tgggcacctg attgtgatga agtcgtaaag
    16801 gaaacgcctt tccgtttcaa ccgtgacgac gactgtccta tcgtttgtct tcttggtgtc
    16861 ggcttttacg atcaaggctt cgcaaatcgg attgcccgag cgatcgagac cgatccattt
    16921 ttctcccgga acgggaacgg gaataaattc ataaggaatt ttgaaaatcg ccttgtcgct
    16981 gtcggtttgg gcgacgatga tggcaagccc cgggcaactg cttatgcaga taccgcatcc
    17041 ggtgcactta tcatgatcga tttggggaat cgaattgatg tcctctccga tcgtgatcgc
    17101 tttgaaaggg cagcttgtgg aacaagggtt gcaagggatc tcttcgtagc attcgaggat
    17161 agctttaggc cttgcaagca cggcctccga aggaaatttt tgtcttacct gttcctgaga
    17221 aggaacgccg tttttcagaa gagtcatgag cgttcaccga gcctttccaa gccgcgtctg
    17281 atcttggttc cggaaggacc ctggcgcaaa tcttgcaatt gtttctcgaa atcggcaacc
    17341 aatttgtcga aatcaggatg cctgtatccg agattttccg ctgcgaaaag accggcaagt
    17401 gcgccttcag ccatggcgct tgacgcttcc tcgatgccgg aaacgtcgcc acatgcgaag
    17461 acattgtcca tgctggtttc tagtctttta tccaataacg ggacgagacc gcccagttct
    17521 ttgacatatt tcattttgac tcccgccatc gacatcagtt gatgaagcgg tgacaagcct
    17581 acggcaattg tcaatgtgtc acaatagatt tctttttccg tacccgggat ctctttccac
    17641 gattcgtcca gcgatactgt tacgactttt tccagttctt tgtccccgat cgcttttttt
    17701 attgttgtcg acacataaat cggaacacca agtctggcta tttttgccgc gtgtacctta
    17761 tatccgccga tagtgggggc tgcttctaaa attgccttta cggtgactcc cgcctgcatg
    17821 agttggtatg tcacgatcaa gccgatgttt ccgcttccga ccatgacaac ttccttgccc
    17881 gggagcacgc cgtacaggtt catcagcgtc tgaaccgcac cggctccgaa aataccggga
    17941 agatcattgt tttcaaatgc taaaaatttt tctgaggctc cggttgccat gatgtatgcc
    18001 tgcgcatgat atttccggta ttcctcgttt gataaagttg tcaatacatg atccttatat
    18061 aatccgacag ctgtcgtatc agtcaaaata tccagcttgt catggtattt tcccaaattg
    18121 ctttttaaga ttccggcaat gtcaaatccc cgtgttttgg catattgttt ttccgaaccg
    18181 aaaaatttgt gggtttgttt cgccagctgt ccgcccgggt atttgtttct gtctacgatt
    18241 gtgacaccgc atcctgcttc caaggcaacc ttggcggcgg ataatccggc cggaccggcg
    18301 ccgattatca tcagttcttt ttcggtcatt ctaacctgcc cagtcctttc tggctttcaa
    18361 ccaccatgcc gtctttcagt tcagtaatgc aggttttgac attggggatc ccgtctaccg
    18421 tcatatggca cgatccgcag ttgccgatcg cgcagtacag tccgcggttt cttccgtctt
    18481 tgatgctctt tgacaacact ttgatgccgg atgcgtgcaa tgccgccgcg attgtgtcgc
    18541 cttccttgcc cgacatcggt cttccatcga aataaaagtc gattttccgc ttttccgtga
    18601 cgtccagaat tggatgtttt ttcagtctca tcttttcacc gctctcacgc tttctatcat
    18661 aacataaaac gcttacattg gtaaatgttt tttttattaa aaatcaggat caatcctgat
    18721 tttcaaaatg ttcattcgcc tattttttcg gtattcgtca cattttcgga tccagcatgc
    18781 aaaacacgtt ttccagtgcc gtaacggcac ttgtcatagc ggttttttcc atttttgaat
    18841 aacccatcac aaaagtcgaa gccaaaggga ttttttttag acaaaattcc ctgagtccct
    18901 ttattttaat ccctgctttt cttgcattct ccatatattt tgattcgtcg atttccgact
    18961 gtgattcaag caaaaaatgc aatccggctt cttctccttt gataccgaaa ttttctgtta
    19021 atctggattg cgataatgca tatatgagag tgtccctttt tgatttatat atttttttca
    19081 ttcttgcaag gtgctgttcg aactttcccg tattgatatt gtctgccaga aactgttgga
    19141 caaacacggg aacaggacat gtgatatagg aatagtccga ttccaatttt ttggctaaat
    19201 gatccggaaa taccatgtag ttgactctca aaccgggaaa caggcttttg gcaaacgaat
    19261 ttacatagat gacacggtca ttgaaatcaa gacttttcat cgccctgatc ggagcgccag
    19321 aaaaacggaa ttcgctgtca tagtcatctt cgatgatata tctatcgttt tttatttccg
    19381 cccatttcaa aaggactgaa cgcctggatg ccggcataac cattccgcag ggaaactgat
    19441 gtgacggagt gacatggact agatttgctt cggattcagt caacatatcg atttttattc
    19501 cgttttcgtc caaattgatt ggcactgtcg atattcccat cgcttcgtac attctcatta
    19561 atttgggata tccgggattc tccacgccga tttttcggtt gtctcctaat aaaagtctta
    19621 ttaacatgat ggtgtattca gttcccgaac cgattatgat gcttcttgga ttcgcgctta
    19681 ttcccctgta tctgtacaga tatccggcta tctctgtttg taagtattca agtccggatg
    19741 gagatgatcc gtttatatcg aataaaatcc gctcggcaaa aacatttctg aattcctttg
    19801 accaattttc gtagggaaac tcgatatcat cgacaacatt ggtcgaaaaa tcatatgcga
    19861 catcggcttt gtttttcttt tttataacaa tatgctcata ttgtttaacc cgatctgttt
    19921 tgctatattg aaaaaacaca ctgacatcct cgacataata tccgctcttt ggtttcgagt
    19981 atatatagcc ttcggcagtc agctgtccgt aggcgttttc aactgtctgg acactgaccc
    20041 caagatcttc ggctaatttt cttttggacg gaattttttc ctgcggatat attttacctt
    20101 ccagtatggc gtcgcggatc tgaatgtaaa gttgcatata caacggttcc ttgccgtcgg
    20161 tattcaaata taaagttatc atatctgacc ttcttatatt atgtcattct ggctatttat
    20221 caaatgtcag ttatcattat acaataattg tggatatttg aaaagcagac ttataaagga
    20281 ggaaacaaaa tggaaaaaac aagatatgac ctgaacaagg agttggctca gatgcttaag
    20341 ggcggagtga ttatggatgt aaccacgccg gaacaagcca aaatcgccga agcttccggc
    20401 gcctgcgccg taatggctct cgaaagaatt ccggccgaca tcagaaaggc aggcggagtt
    20461 tccaggatga gcgatcccaa actcataaag gacattcaag cggcggtttc cattccggtt
    20521 atggcaaaag tccggatcgg tcactttgtc gaagccgaaa ttttggaggc gctcgaaata
    20581 gattatatcg acgaatcgga ggtcctttca ccggccgacg acatttatca tatcgacaaa
    20641 acagctttca aagtgccatt cgtttgcgga gcaaaaaacc ttggcgaagc attgcgaaga
    20701 atatccgaag gagccgcaat gattcggaca aaaggcgaac cgggaaccgg tgatgtcatt
    20761 caggcagtca gacacatgcg tctgatccaa gcggaaatca ggcgggtcgt atcactttcc
    20821 gaagatgaaa tttacgaagc cgcaaaagaa ttgggcgttt cgattgatct tttgaaagat
    20881 gtcaaaaaga caggtaaatt gccggttgta aattttgcag ccggaggaat cgccactccc
    20941 gccgacgccg cgcttatgat gaaactcggg gcggaaggcg ttttcgtcgg ttccggaatc
    21001 ttcaagtccg gaaatcccgc caagcgggcg gaagcaatcg tcaaagcggt tgccaatttc
    21061 aacgaccccg ccattctcgc tttggtttcg gaaaatctcg gtgaagcaat ggtcgggaca
    21121 aacgaggagg aaatcgcgct gttgatggcc aaccgcggtg aatgatctca tggaaatcgg
    21181 tattttggcc cttcagggag catttatcga acacgaacag gcatttgaag atatcgggat
    21241 ttccactcat gaaatcagaa atcgtaaaga tctggagaaa ccgatggatg gtcttgtcat
    21301 tcctggcggg gaaagcacct cgatggttaa gcttctcaaa ggactttcca tgtgggataa
    21361 gctcaaggat atgcttgaca agggactccc cgcattcggc acatgtgccg gactcatatt
    21421 gctcgccaag gttaatgacg gacgccaaga ttcctttggc tcaattgaca tcgaggccaa
    21481 aagaaatggc tacggccgcc agatagacag ttttttcgct tatggagaaa ccgaggacaa
    21541 tacattattt ccgcttgttt tcatcagggc gccaatcata acgtcaatcg gccccgatgt
    21601 cttcaaagca gccactatgg gaaaaaatat cgttgcctgc cgcaacaata atattcttgt
    21661 atgcgcgttc catccagagt tgacggaaaa ccgttatttt cacagattat tcgttgatat
    21721 ctgtaagcgt taattgtttt atactaatta aaggaaacgg ccccgtctgg cgaattgcat
    21781 ttgtctgacg gagccgcttt ttatatttgt tattcaaata tattttcttt ttacccttgg
    21841 tgatatgtca gtaaggattt cataagatat tgttccgatc ttttttgcga tggtgtcggc
    21901 atttatatca ccggtaccga atatctccac gaaatcccct ggtttcaatt cgcctttttc
    21961 tattcccacc atggcaagat ccatacagat ggttccgata acgggaaatt ctttcccgtc
    22021 tttggatacg gaaatgatac cggaaagcag tcttgaaaga ccgtccgcat agccgatgga
    22081 gatagtcgca acttccatat cttcttttgc aacataacgg catccgtaac ttaccggcat
    22141 tcccttctta agtttagcga ccctgaccac tcttgctttc agagtcaaaa ctggtttcaa
    22201 ccgaagatcg gtttttaccg gaggcaaacc gtataaggcg attccacatc tgaccatgtc
    22261 gagatgcatc tctttatgaa gaatagttgc agcgctgttg caacaatgcc tcagaggaat
    22321 gtcgacgcca gctttgtcta acttatccag cacttttgaa aacaacgaaa attggagatc
    22381 cgtaaaggga ttttccgtgt tttcgctgat agcgaaatgc gtgtaaatac cggtcgttat
    22441 acaaaatccg gttcggataa tttccataat ctcggcaaaa gcggtttccg tttccgcttc
    22501 cgtccggcat aagatcccca tccttgacat tccggtgtct agatttatat gcgctttgta
    22561 tctaatttta tgcttggcgt attctttcgc ctgatttacc gatatcatcg tcggggttat
    22621 cccgtactcg gtcaaaaagg aaaagtcgca cgcgtcgatc aatccgaata ccaggatttc
    22681 tcctttgatc ccggcctctc ttagttcaat cgcttcctcg agagtcgcga caccgaaatg
    22741 tgaagccccg atttcggcca gcttgcaagc ggtcttaacc gccccgtgtc cgtaagcatc
    22801 tgctttgaca accgccataa cttccgtttt gggacccacg cgttcactca gaatgcgata
    22861 attttccgcc aatgtctcga gatttacctc ggcaacgcat ctgttttgta tttctgtttg
    22921 tttcatcggt atttcacgcc ttcccgaagc attgatgcag tatataaata tttattatag
    22981 tctaattata atgccgtttc accatgttgt aaatattcta tagcatttgc aaaacgaaaa
    23041 ttttatacat caatattaat ctgtttttac attttttctt atcatcagtc cattctttat
    23101 gatacaattg gcttgagtag aaatctaggc ggtgaaccta tggcgacagc acagtgatcg
    23161 acaagctcaa caatggcgaa gtcgacgcca caagaatgag ccagtatgcc gattgggtcg
    23221 aacttttgtt cgaatattcc gatcagacaa tcctcaaaac cggcacatac gatgaccaag
    23281 tacaaaaatt cgattctgga catgccgctt ttattcatca gggcaactgg atcgatccga
    23341 acctggacga agcgacattc acaatgggat atgctccaca tgcttccgta tacggaactt
    23401 gcgacagcat tttcatcgcc gccccgtcat actatgtcat caattccgat tccgagaaca
    23461 tcgacaccgc caaattgttc ctgaatgatc ttgcttcgac aacagccgga atggattaca
    23521 cggtcaatca ggctggcatg atctcggcct tcaaatcctg cacgttgctt ccttccggag
    23581 ggctttcaca ggctcttgtt acctggatga acgccggtaa ggcttattca tggtggcaga
    23641 atgacatgcc ttcgaacttc ggcatgggaa cccttggccc gatctacacg ctttatgcca
    23701 acggaacaat cgacaagacg ggattcatca ctcagattac cgccgctatc gaagcactcg
    23761 ctccagaaga ataattcaat atcaggaatc caaaaaagac cgcgatcgat ttcgcggcct
    23821 ttttttatct gaaattctat tcgagttcaa atgccccggt ataaagctgg taatacattc
    23881 cttttttgtt tatcaatttc tcgtggtttc cgatttcgat tattcttcct tgatcgagta
    23941 cgacaatcac gtcggaattg tggatggtgg aaaggcgatg ggctatcacg aatacggttc
    24001 ttccctccat gagccgatcc attcccttct ggactatggc ttccgttctg gtgtcaatac
    24061 tgctggtggc ttcgtcaagc actaatatcg gcgggttggc gacggcggct ctagcgatcg
    24121 acaacagttg tctttgccct tgggaaagat tgtttccccc cggtgtaagc atggtgtcgt
    24181 agccatccgg caacattctg atgaagccgt cggcgtttga tcttttggcc gcgtcgatga
    24241 tttcctcgtc gcttgcgtcc agtttaccga agcgaatgtt gtccttgatc gttccggtaa
    24301 acaaattggt atcttgcaaa accattccca gcgaatgtct taaatccggt ttcctgatct
    24361 tgtttatatt tattccatcg atccggatct ttccgtcggc gatgtcatag aaacggttga
    24421 gcagattagt gattgtcgtc ttacctgctc ccgtatggcc gacaaaagcg attttttctg
    24481 ctttatccgc ttgcaaggtg atattgtgca aaacggtttt tccgggttcg taagcgaaat
    24541 ctacgtcgtt gaacgtcact tttccctgaa caagcacata ttcgagcttg ccgtccttgt
    24601 gcagatgctt ccaggcccaa agatttgttt tttcttttgt ttcctttaga gttccgtccg
    24661 gttgcctttc cacattcacg agcgtcacat aaccctcgtc ttcctctttt ttcgcatcca
    24721 tcaattggaa aatccggtcg gcgccggcca aagcaacgat aatcgagtta aattggttag
    24781 caatctgatt gatcggcatc gtgaagtttt tggatagttg catgaaactg gcgacgactc
    24841 cgagagtgat tcccggaaag cccaagatac aaagagcaga tccgatgaca gcgataatga
    24901 catattgcag gtttccgaga ttattcatca ccggcatcat cagggtggcg aacttattcg
    24961 ccatcgtcgc attaacccgc aatttgtcgt ttaaaacatc gaactgttcc ttggtttttt
    25021 cctcgtgaca gaataccttg atcacttttt cgccttcgac catctcttcg atgaaaccat
    25081 tggtctcgcc aagcgatttc tgttgggcga caaagtattt cccactgaca gaagcgatct
    25141 tcttcgtaat catcatcata agcacaacgc agacgatgac aatcagtgtc agaacggcat
    25201 tggaaaacaa catggcgaag aatatcacga tgacggtcgc catcgaggag ataaactgag
    25261 gaacgctctg cgccagcatc tgtctcaggg tgtccgcatc gttcgtgtac cggctcatca
    25321 gatcgccgtg ggtatgaccg tcgtaataag aaatcggcag atcctgcata tttgagaaca
    25381 tttcgtcgcg gatattcttc atgattccct gggccatcac gaccattgtc cggttgtaca
    25441 gataggaaga agcgatccag acgacgtaga tcagcgccag tatgccgatc gccgtcagaa
    25501 gtcccgaaaa taccgggtcg gaaacgccga tcatcggcgt gatgtaatca tcgatcagaa
    25561 tccggatgaa tattgatccg gcagcaccgg cgatggcact gaataggata cagaccaata
    25621 cgaatatgaa acgccatttg taatcgcgga atatatatcc gagcagacgc ttcagggttt
    25681 tgccatgctt tttaatgttg aatttctcgc ggggtcccgc attgaaaggc ttaggcattt
    25741 tccatcaccg ccttgctctg cgattcgtat acttcgcgat aaatcgcgtt ggtggccaaa
    25801 agatcctcat gtgtgcccat ttgattcaat tgaccgcctt ccataacgat gatggcgtca
    25861 gcgtcttcaa tggacgaaat tctttgggtg atgacgattt tggtcactcc ctggagttcg
    25921 ttcttgaaca tttttctgat ttcggcatcg gtcctggtgt cgacggcgct cgtcgaatca
    25981 tctaaaatca gaatcttcgg attctttagt aatgccctgg caatacaaag cctctgtttc
    26041 tggcctccgg aaacgttagt gcctccctgt tcaatatggg tgtcatatcc tttcggcatt
    26101 tcccggatga attcgtcggc tctcgccatc cggcaggctc tgatcatttc ttcttcggtg
    26161 gcattttcgt ttccccaccg gagattgtcc ttaatcgatc cggagaacag cacgtttttc
    26221 tgtaagacga ccgcgacgga attcctcagg gcttcgatat cgtaatcctt gacattgttc
    26281 ccaccgacat agatgcttcc ggcagtggaa tcgtacaatc tggcgatcag ctggactaag
    26341 ctgcttttcg agctgccggt gctgccgatg ataccgatcg tctggccgga ttttatttta
    26401 aaattgatgt catcaagcac tttccgttcc gagttttccg agtagcgaaa atcgacatga
    26461 tcgaatatga tgtcgccgtt atttacgttc gttaccggat tgttagggtt ggtgatgtcg
    26521 cttttttcat cgagcaattc ggttatcctg atggcggaag ccctggaaat cgtgatcatc
    26581 acgatcacca tggaaaccat catcaggctg gaaagaatca ttccggaata ggtgatgagg
    26641 ctggtcagtt ccccggtcgt aatcatgttc ccgccgattc ctttgacgat tagcacggct
    26701 ccgaaatagg agatcaccaa aatgcatacg tacatcgaaa attgcatgag cggactcatg
    26761 gccgccaaaa ttttttctcc tttggaaaag tatttataga tttcctgcga tgaggcgcga
    26821 aatttctctt tttcataatc ttcccgtaca taggtcttaa cgaccctgat gccggcgatg
    26881 tcttcctgga cgatgttgtt catcccgtcg tatttcttga acacttccgt gaaaaccgga
    26941 aaaaccgatt tgatgatgaa aatgaatccg attatcaata ttggaatgac aagcaggaag
    27001 gtaagcgaca atgtaggact gatggaaatc gtgatgatga gggcggccac gagcataatc
    27061 ggggctctga aagctatcct tatcaacatt tggaaggcat tctggacgtt ggaaacgtcg
    27121 gttgtaagtc tggtaatgat cgacgaagtc tggaatttgt cgatattccg gaacgaaaag
    27181 tcctgaacgt tatcaaacat tttttttcgg atgtttgccg caaatccggc cgaagcattg
    27241 gccgagaaca cgcaagataa cgaaccgaag cccagagata tggatgcgct tagcactagt
    27301 aacaagccga atttcacaac ggccgacagg tctccgggag ttatgccgtc atcaatcagt
    27361 ttggccatca ataacgggat gatcacttcc atgacgacct ctaaagatat catgagcggt
    27421 gacatgaagg tatcaaattt gtatttcccg atgttctttg caagtttcct aatcattttt
    27481 cgtctctccg ttatagtttt ccatgtttcc aatcaggata tcgagcattt ccatgagatc
    27541 ggccattttc ccgtcgtcta tgcctttcgc cagttttctt tcaaactggg taaggtctat
    27601 ttcgtcctta tgcgatatct ctatcgcttt ttctgttaga caaatctgtt tcaacctttt
    27661 atcgcctgat actgcttttc tttcgatgag agcgttggat tccatcaaat ccagagttcc
    27721 ggacatcgtg gatctgcgga cgttgaattc tttttcgagg tccttttgaa atatcggcaa
    27781 gtcatcgtta tgtctgatga atttcactat gcgaaaatgt aaaaaggtca atccttcagt
    27841 gcttttggat atctcttctt cgcggttgcg tttgattaaa tgggaaagaa atattatctt
    27901 gcatccgagc aagtcgtttt ttttcaaaat acgctcacat cccgtttttg ttaggttgct
    27961 aacattatac cacaatgtta acatatgtca atatagaatc ctggctgttt aacactgtct
    28021 ggcatatctt ttgatattaa ttgacgggat atcctttttt ctttgatatg gatctgtttt
    28081 tgtgataaaa tcaaatggaa ggtggacata tgaagaaaaa gcacatcgta gtcagtccat
    28141 tccaacaata caggcagtca ctatcttccg aaagcaatga attgctgacg gatttttttg
    28201 ccagtttcga cgaacacgta ctgttaccgg aaaaaggaaa atccgaaatg cgttccgatt
    28261 ttgaaaaagc catcctgtat ttcgattccc tcggagtcag gctcgacaag tctcttgaaa
    28321 ggctcgatac gaaaaacctg ggtggttttt attcgcgtcc ggcgatcagg tggtttcctt
    28381 tggataccgc cgcaaaaata tatccgcttt cgatgcgtcg caatcaaatg gccgttttcc
    28441 ggctttccgc ctatatgaag gaaaccgtta atccggaaat tctccagatt gccctcagct
    28501 tcacgatcaa acgcttcccg tgtttctcaa ccacgatcaa gaaggggttc ttctggcatt
    28561 atctcgatac gactaaaaga cgctttgtcg ttgaaaaaga atatgacatt ccctgccgtc
    28621 ctttggacgt ttccttgagc ggttcccaaa ctttccgctt gctatatcac gaaaacagga
    28681 taagcgtgga aatctttcat attttggccg acggatacgg cggtatggta tttcttaaaa
    28741 cacttgtcgc cgaatatctt cgtttgcaag gcatcgacat aacatatgac aaaaccattc
    28801 ttccgatcaa tgaaactccg gtggacgggg aaatgtctga tgccttcttc tttgtggaac
    28861 caaaaaacaa ggcttccgga tttctgaatc gtccttccgt ccaattgagc ggatcgcttt
    28921 cacggaccaa accttgccgc gtattacatt tcatcatgga taccgacaaa ctgaaagccg
    28981 ttgccaaaag caagaattca actatcactt catatcttgt cgccaacatg atgctggcag
    29041 gcaaatcagc caccgacgag ccttccggat ccatgtcgat ccaagttccc gtcgatatgc
    29101 gtaaatacta cccttccggc actctgcgca acttttcgct ctattgcggc atcaagctcc
    29161 ctttgacaaa gataaccgac tgcgaatcaa ttttaccgga gatatccaga cagttagttg
    29221 aaaaaggcgc tttgggcccg atgaacgaaa tgatgaacgc cgccaaggga ctcgtccgct
    29281 ctttacggtt cgttccgctt ttcatcaaga atccggtagc tcgcatcata tacggctttc
    29341 tgggagatca ggctttttcc aacacgcttt caaacctcgg catcgtcaat cttcccgaag
    29401 agatggcttc gcatgtcgtc ggtttcgatt ttctgctagg cacatcgatc acaaaccgag
    29461 tatcctgttc tgtcgtttca tacaacaaca agaccacttt ctcgatcgcc aaactgacac
    29521 ttgacccgtc ctatgaagaa actatgtaca agttactgaa gtcggacggc atcgatgtca
    29581 tagtggaagg gagcggtatt tatggaagtt aagatcactt atccggcccg tgaccgtaaa
    29641 aagagccggg cgcttctatt aagaagcgtc gtaaagtggc ttttcgtcgc ttccgcttat
    29701 gtctgcccga tcatcaacat ctcggcaggc ggtccggctt ggagcattta tgtgctctgg
    29761 tttttgcttt tcgtttggtc atttgctttt tcacccgatc tcgtggaata caatctgatc
    29821 agccaaacag cgaaatttct tgttttcacc tgcgttttgc tggcgctgat cgatatcatc
    29881 cactccctcg ggtgggcaat gtttgtcata ccgatcatct gtttcggcgg attggccgtc
    29941 atcgcggtgt tcttcttttc caacatcaat atccaaaggc agaatatcat gccgatgatg
    30001 tgggtgatca tcgcctcgat catcggaatc gtcgttgcca tatccggatg gaaacaaatg
    30061 agttggccga tgattgtctt gatttcactt tctgtcgcca ttttactggc cagcgccgcg
    30121 gttctcggaa aaggattcat cagggaaatc atcaaacgtt tcagcatcaa atgattcttc
    30181 tgctatgttt tcaataaaaa atccgctttt gcaagcggac ttttttttat ctttctttct
    30241 tacttgtcga tgactccacc ggcgcacaca agttttgtgc cgattccgat cgtatccccg
    30301 accgggcagg tcttctcgat aaacttgacg aatttgtcga tcttttcctg aggttcgtcg
    30361 gtcttgaagt gcatcgtgaa acggatttcc tggaatccgg gtcttacatc ggatttaccc
    30421 ataaagccgt ccaaatcgat gtctccttca agttctgtat ggaattcttc gaatttcact
    30481 ccgttggctt ttgcaaaagc tgctgcgcaa attgcctgac atcctccgag agcaaccagt
    30541 aacgcttcca ccggattcat tccggtatct gacccgccca agccggcggg ctcatcaaga
    30601 atgatcttga atcctctcgc tgttccttcg gtttgtagac ccttttctgt tttttttaca
    30661 cttgctttaa atgttgttat catttcaaga atctcctttc accattgatg attttggaat
    30721 taacaaccat cttgctaatt ccatactaaa tatacgataa tttcgggcaa atgtcaataa
    30781 ttttgacttg cagaaaacat tttcactatc tctgtttatt acaagtttaa ttatttttta
    30841 cttattttgt tatccgacta taaattgacc gtcttttctt attatattta ttgttatttt
    30901 gtcgcgttca ttattttcta ttcattttaa aaacgatttt gcttgtcctt ttattaagat
    30961 aattatcaaa atttagatat tattcaaatc atgttttcgt gatataaaca tatatcatac
    31021 tcttgttttc aaaataatat cttctcaaaa aaggctgaaa atggccgttt ttgcaatcta
    31081 aaaacgtttt catgtaaaaa ataagaaatt tttgtgtttt ttttcttgac atcgaattgt
    31141 ttatccgtta taatcagtgt cgtaaaaagc aaaactaacg aaagttagtg acgcaaagct
    31201 atagggcctt cgcaagatgg tagccagttg ccgttgtctc aaatcgagat gagggcattt
    31261 tttttactct caggaccgag aaaactacat aggactgaaa aagccaaagt cggagcaatc
    31321 cggtgacgga aaactatagg gtcccattcg ggatagccag ctaccatccg ttatttactt
    31381 tggcttttat tctatcttat ggaggtatta aaaatgaaaa aggtatttgt cgcaatttta
    31441 gttgcactgc ttctcggggg aaccgctttg gctttctcat ggtgggataa tctttcccgg
    31501 gaacaaaatg aaactttgca gatcggtgaa ggcgtcacat tggtcgtaac ggccgtcgct
    31561 accgcccccg cagacaaggt tctcgtaccc gccggtgtca caatgacggc ggatgatgtc
    31621 gatcaaattc aactaactta caacgtgaat ttggatcaga cagtcataga cccattgacc
    31681 ctcagcgtcg tcgcaaccaa tgttttgatc ggtggttcaa gcacttatgc cgatctgatc
    31741 aatatcgata ttacccaggc agcaactact gtcaatgacg ccaatgtctt ggtaactgtc
    31801 actgttagtc ttgatatgcc ttcgacccag gccatttacg atgaaatcaa gaacggccaa
    31861 atcactttct ctctcacttt cacaggtgaa taagcatcaa tcttaattca aaaaaaaaaa
    31921 aaaaaatata atataaaaat taaaaggaga attatcatga aaaaaagaaa tatcttgtta
    31981 gtagttatca tcgcattatt gtttggttcc agtgtcgccg cttatgctta ttgggataat
    32041 ctgcagcaga acactaacgg taattatgtt gtcggatatg gcgtaagatt ggaagttccg
    32101 accgttgtcc aggatgaaag agctttggtt cccgccgaat ctttctatgc cgcttacgaa
    32161 gctgattaca ccacatccta tgctttcaca tacaccctga gtcttgaaga cgatcttcag
    32221 tacggcatgg aagccgatct ggctgtcgat atcaccgatt tccaaatcgc tggaatcgcc
    32281 gaactcttca acagtgagga aggattgttc acagtgacag tcggcaccga tgaagccgcc
    32341 gccacaactt cttccaccgg agaatgggat ttcaccgatg cattctatta caaccacaac
    32401 accgtggaag tcaccgtcac catcacattg gcagataacg gcagcgccgg atttgatgcc
    32461 tctgattatg aaagagtcgc cggtaagaca acaaacttcg caatcggatt ccttctttcc
    32521 aacagcaaca gttctaatca acccgctgaa taatacaatc acaatttcac ggagataaat
    32581 catggctcag gaaaaatcgg taccgtcaaa taaaaaaccg aaatccagta tatctaaagg
    32641 tatacggttt ttcctttctg tagctattat tcttggactg ggatatgtag ttttcaatta
    32701 cgtccccttt atcgccaagt acgattactt tgtgatagcc accggaagca tggagccggt
    32761 tattatgacc catgacgtcg tgatcatcga cacatcagtt gatccggata atctttcggt
    32821 gggagatatt atcgctttct atactgacat caacggtgat ggaaccgacg agatcgtcgt
    32881 gcattacatc tactcaatca gcgaagacga aggcgtcaga acattcctca cccatccaga
    32941 agtaagcgaa catcaggatc cctggacttt gtacgacgaa gatatcgtcg gaatccatgt
    33001 tcttacgatt ccaaaaatcg gaggtttcct gctattcgcc caatcattct tcggccggat
    33061 cgtgctgatt tcggatattc tgattctctt ccttctgctc gaactggttt ccgactcgaa
    33121 caaaaccaag aaaaaacccg atgattgtca ggaacaggaa acattagaaa aatcaacgga
    33181 cagttcttcc gatgaaacgg aagatgattc cgatattcat tccggaaatt gatctgtgat
    33241 ccataagata caagcctaaa acaaaggagg tgtatgacca tgaaaagaaa aatgctagtc
    33301 gtaatactga caatcgctgt gtttttcatg tccttttctt ccataacata tgtcggatac
    33361 agctatttcg acagtctttc ttcaaatagt cagatcactc tccaaatagg cgaatggaca
    33421 atggcgccgg acgaatggga tccaaccctc aaatatgaca aaggcgatca ggttacctat
    33481 aacggcgtga tctatgaagc cttccgcaat gtgccggcaa acagggctcc cgacagcttc
    33541 cgtggctttt tctactggta tatcgtttcg tgaaacaaag aaaacaaacg caaaccatta
    33601 ataaaacagt cctgaaacaa cggactgttt tttattgaca gattaatttg gattataaag
    33661 atgactgctt gcttcttatt tcgcagatat ctgactgaag tgaaaatata atatgaccgg
    33721 tatagcaatg ggaacggcat acaaaagcga attaattacg acttccagat accacggggc
    33781 actgcagaca aaacaattat atagataaat ggaagcgata atggtatgta tcacatatgc
    33841 gccggttatc aaaatagcgg tatacataaa tacttttttg agtatcgccg aatcgaaacg
    33901 attctgaaga aacctgatgc ataagtccat aaggaaatat gccgttacaa acagaacata
    33961 atgcccaagt ccgattagtc ccaaagtgga ttcgggaagc aagatgtcat aaaacatgta
    34021 aacgggaatc atggccagtg ccgaaccgat cagtaaatac ttccaggata tttctcttag
    34081 gacgcttatc agtccgatca aaatataaat caatggcaac aagatataat caatggccgg
    34141 atcatcgtat ctgttcaagg taaaaaaaat gtagggtatc aaaatcccgc tcaaggaata
    34201 ggcaaaaaat atggcgacgc tggaaataat gtcgttgaat tttctgattt tctcggtatt
    34261 gaccatggcc aatgcgattc tgtcgtcttc gttcactatc aaatcgagag acactttgaa
    34321 aagtgtcgcc aactgtttta ggttatcgat gctgggaatt cccttatcgg tctcccattt
    34381 ggaaatcgcg tttctcgtga catatatttt cgaagccaat tcttcctgag tcaaattgtt
    34441 ctcctgtctc aattgtttga tgttttctcc gatgctcatg ttttcacctc tcgcatataa
    34501 tatagcatat caggtcagga catcatagac acttatcgtt acgtataaga aaagtggtga
    34561 taacatcgta taatacttaa tcttgtttct tggttttcat aatctttatt tttgcgataa
    34621 aaaatcgctt tcggataaga aacattcata tttaagcaca ttgtgtttca aattttcact
    34681 cgttgctaaa agatgtcttg cagtttggaa atgtaatcta tcgtcttgtc agctatgtct
    34741 tttccgtaat tcgagtcttg gtattttgac aattccgttc ggatccagat tgttttcatt
    34801 ccgagagatt tcgcgggttt gatgtcatta tctagtctat cgccgaccat aacggattct
    34861 gtcggtaagc aaccggccag ttcgaacgct ttttcgaata tccgcttatc cggttttgac
    34921 actccaagtt ctgccgaaga tacgacaatg tcgaagaatt ttaatattcc ccacctatca
    34981 agtctttgtg aaacgcccgg catttgattg gcaatgattc ccagtttgta tccgcgattt
    35041 ttcagatagt ccagcgtttc aacagaatcc atgaacaaaa tctcgtcttc caagtgccag
    35101 ggagttttct taagccggaa atatttaatt gtttcctgga tgtcgtcaag acctttgccc
    35161 gcgaattcaa ttcttttgtt ttcaaaatca tcataggaaa ccttggttcc ttctgtcatt
    35221 tcccggatac gatgaatata cgctttcgtt tcatcgatca gcgtggaccc cagatcgaaa
    35281 aagatccatt ttgttgacat atagtcacct ctagtttaat tgatcatata aaatatgata
    35341 tttattagtt tatgctaatt atactatgga tgtttaaaaa aagtgatccc gaatataatt
    35401 aatccaaaat caaaaatatt tttcaatatg gtataatatt agcattaaac aggttggtgg
    35461 aaaaacatga aaaaattaag caaagcggtt ttcttacttt tactaagctt atatagtgtt
    35521 ttcttcgctg aaatcatcgc gggaagcagc aactacccgt tgtttgatct ttggggtttg
    35581 ttcgtggtga ttcccttata tggttttcat gtgataatct tgttgacgat catcattcgt
    35641 tttcaaaaag gcaaagtcat tccttttcgc acaatgtatt tcgccggcat gctattcgca
    35701 ttatacgaag catatataac gaaggtgttg tggatcgggt tttccgacga tccccatatt
    35761 ttccttggga tcggattgat cgaatatctc atattggtgc cgttctggca tccgatattc
    35821 tccttcatta tcccgtctgc cctgctggaa cgaaccttgc ttaaatcatc atacatcttt
    35881 gacggactgc caaattttat gaagcatatc cttaaccgca aggcgggtat catatcatta
    35941 ttcgccatta ttggcgtatt cgccgcaata aacggaactt ttccgattct tatgctatcg
    36001 atgatttcga tgcttgtgcc gatgttgctc gtcatttatt tattgaaacg aagacatttg
    36061 accaatgagt atacgcttaa ggatatcatg ccccgaaaac aaacgttcat aataatgtgc
    36121 atcctgcttt taggactata tgtattcggg acattgtgga ttcgtttcga tgccataact
//
LOCUS       DAOPJL010000011        35924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1249_length_35924_cov_7.235161, whole genome shotgun sequence.
ACCESSION   DAOPJL010000011 DAOPJL010000000
VERSION     DAOPJL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 35924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35924
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_35924_cov_7.235161"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLP02_02605"
     CDS             complement(<1..1239)
                     /locus_tag="PLP02_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42376.1"
                     /translation="MFTINIKGETLKFENKVRLTELASRYEGTYYAAKVNNRLRELTY
                     VIGFDADVDLLDYTYSDTARIYATSMRYLICMAFRRVFPNLQIKFSNSIAMGIYGRAI
                     NGIITPDMIQLVLTEMQNIIKANYPITRKKISISEAKKYYRSKGYIDKVETLKYRKED
                     VNIYECDGYCNYMYGYMVPSTGYLTYFELFYYSPGFLVRYPRIEEKGQIPEFSDSPSF
                     LRVLNRAEQWAINCDADMIYKLNRKIEENRQVEFVNMCETKHNNQLCELGEIISKDID
                     NIRLIAIAGPSSSGKTTFSRRLEIELLTRGIKPLMISIDNYYLPVDKAPLDEFGEPDL
                     EHVKALDLELFNRNITDLINGDSVSLPFFDFRKHMRSFTPPIHIESHNPIIIEGIHAL
                     NDVLTEFIPASQKFKIYISPL"
     gene            1387..2196
                     /locus_tag="PLP02_02610"
     CDS             1387..2196
                     /locus_tag="PLP02_02610"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPF42377.1"
                     /translation="MLLSTFFSSTGMDESPTAYLFKWPHFLYMFLVITGFIVLMQIFV
                     HKSDKTRHIFLIVIAVIMLLLKYSGEVLYIWEWNHYGDTISSSSHPFWDVRTFISFQL
                     CGINNVLLPLVIIFNLKPMRDFIYTTSIIGGLAAMLYPVGILFGDPFVITFPMIRTLV
                     VHFLLLFTPCFMIASGDYTLKLKSWWKTIPIGCIAMYLWAMFGNLVIDPGANNMYLME
                     NPFIGVGIPILSDIPSGLHVLLLLAMVFLGFFIVYMSIGAYQKHRDKKIAI"
     gene            complement(2249..3166)
                     /locus_tag="PLP02_02615"
     CDS             complement(2249..3166)
                     /locus_tag="PLP02_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42378.1"
                     /translation="MKYRELKEIIKDNAAFKLPENRSFLEEAKSHYIPVLSNDRKQPL
                     WHRLMVGFSLALFVVLLGSGLYLDFKITATVTVDINPSVELEINSFRRVRKVIPLNSD
                     GEILVENLTYHSGSLSVVLSDILQTSEALGYSDIDDTALLFGVSGTKPEIEDGISEII
                     SRCEKAGTSSYLIIKTHLMTGDLMASGYSITVTNSLTSDYDIWSLFGSSRCASEEQAT
                     TTTTTAAISTTTGNDTSSDSYNTYTPNYAFSGTINLETLSATYGITEAKMELILIIYA
                     TVSDYQTETGFQELLNMDLSELLALYLSE"
     gene            complement(3170..3700)
                     /locus_tag="PLP02_02620"
     CDS             complement(3170..3700)
                     /locus_tag="PLP02_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPF42379.1"
                     /translation="MPDDLDQLVRKYNAGDTEAFDQLYELTYRQVYFAVLPILNDRSL
                     AEDIVQDTYMKLCACLSGYRERNLLAYVITIARNLAKNEYNRRKREIRIESVEADYSQ
                     VSLADHLEIKAEKEELVKEILDSLDLDEKNVFLLHNLENLPHREIAVILDKPIGTVTW
                     IHAKALKKLKAKFKER"
     gene            complement(3836..4474)
                     /locus_tag="PLP02_02625"
     CDS             complement(3836..4474)
                     /locus_tag="PLP02_02625"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HPF42380.1"
                     /translation="MLLDFFMEYWRFFAIVFGTIALMTIIYLFYNLDSRKKLARMEKQ
                     LPKEADTHERTISLKDLIDQDVIVPNAKEPSALEKKDIYPEQTIFTQKIISPEVSKPI
                     IVEEIAEKKPKIIPEVIQNAEDKPEVPDEKGIKKPEEKKTPKKAAVHKELGRYHVYYR
                     KEDGSWYVKREGSDRILRVLPTQREAIAFATIKAITQNSSYVVHNMDGKIRK"
     gene            complement(4496..5377)
                     /locus_tag="PLP02_02630"
     CDS             complement(4496..5377)
                     /locus_tag="PLP02_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42381.1"
                     /translation="MSASSFQNTVFPCKNPQRYFGVDFFASIFQGSNPTRKFYASPAD
                     IDQTYRPELEDIKHMGMESFIYEMRRIPEGSVIGFDQNPDPYVASINKVGTMKKVLEA
                     IKESNMGLYLETSSENVLEDIPIIVKISENAPVLIAIPVGFVNDEMGRMFEPSEPSFA
                     AKLRLIRRFASENLRVGVILKPVIPFVNDSEENILQILNKAKEAGASFMYPSFGIIIS
                     DEQRPSFYSLIDKHYPGLKNIYMDNFGIRKSISSANAEKLKKAFVFSAKKMHVDFGMK
                     EIIRSVKPTSDMQMKLF"
     gene            complement(5387..5908)
                     /locus_tag="PLP02_02635"
     CDS             complement(5387..5908)
                     /locus_tag="PLP02_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42382.1"
                     /translation="MEAYFELFLKGICTIFILYVSFNTIFRYHTNSSVTDFFLVFSWM
                     IVSFFFIGHYLIVAYVSIIGGILIIYAAMRIYLAYRKTDIRLTYSFSKREADNLKIAL
                     GNASETLPEGKIYNYRNLSYIIITKDLTKDEHKAFVKKLDKEISQVPIHFGYFQYFNI
                     IFALILMASIWRF"
     gene            complement(5928..6479)
                     /locus_tag="PLP02_02640"
     CDS             complement(5928..6479)
                     /locus_tag="PLP02_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPF42383.1"
                     /translation="MGKIKTINIFGSSGSGSTTLARAVAKKYGYIFIDTDDAIWEKTE
                     LPFSKRKSDKDAREYLEARMKTGDKIVISGSFIGWADDIKEKIDLFVYLNLPLEIRKS
                     RIESREKARFGNRVLPGGDLYQKHIDFLEWISLYDETPESLRGKKQHEMWLADVKVPV
                     VRITEPADIDTLVDAVEKRLNLS"
     gene            complement(6643..7011)
                     /gene="rplT"
                     /locus_tag="PLP02_02645"
     CDS             complement(6643..7011)
                     /gene="rplT"
                     /locus_tag="PLP02_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPF42384.1"
                     /translation="MPRVKGGYTTRRRRKSILKLAKGYYGAKHLLYRTANEQVMNSLA
                     YAYRDRRRRKRDFRKLWITRINAAARLNNMSYSRLINGLNLANVEINRKMLADLAVND
                     EKGFSAVCDQAKKALAKTDK"
     gene            complement(7032..7229)
                     /gene="rpmI"
                     /locus_tag="PLP02_02650"
     CDS             complement(7032..7229)
                     /gene="rpmI"
                     /locus_tag="PLP02_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPF42385.1"
                     /translation="MPKMKSHAGTKKRLKRTGTGKLLRASTFHNHLMSHKSPKQNRQA
                     RRKETVGTSDLKRIRHQVPYL"
     gene            complement(7247..7792)
                     /gene="infC"
                     /locus_tag="PLP02_02655"
     CDS             complement(7247..7792)
                     /gene="infC"
                     /locus_tag="PLP02_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPF42386.1"
                     /translation="MIYNINQKPVKKDETPINEEIRYREVLLIGADGTQYGIKQTRDA
                     LSIAYEQGYDLVLVAATAKPPVCKLMDYRKFKFEQQKKAREAKKNQRIVDIKEIRLTA
                     VIDTHDYETKLRNGIKFLEKGDKLKVSVWLPYRAGDMLIRQGKDVLEKFRQRCDEIAS
                     TEKEIAHEGRYLTMVLTPKKK"
     misc_feature    complement(7803..7918)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(7971..9353)
                     /gene="glmU"
                     /locus_tag="PLP02_02660"
     CDS             complement(7971..9353)
                     /gene="glmU"
                     /locus_tag="PLP02_02660"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPF42387.1"
                     /translation="MKRYAIVLAAGKGTRMKTELPKCAYPLLRKPMIAYIIENIRNSG
                     SVDEIVAVVGYKREVIQDILQDAVKYALQEEQLGTGDAVLSAKCLITDPDGFTVILPG
                     DMPLVDEETIEKAISEHIERRHDLTVVTTRIEDPFGYGRIMRNDSGYLEAIIEEHEAS
                     YAQKTINEINTGIYVVKNDLLFPSLAKIGNDNLKKEYYLTDIVKIFKDENRVIGTYFL
                     KDSAKAMGVNDLYALSIAEAKLRLSINKRLMQSGVAMINPETITIGQNVVIEENVLIN
                     PNTYITGNSVIKKGAQIGPNTEIHESIIGERVICRHSLVYNSIVHEDTSVGPFAHLRD
                     GAEIGPKNRIGNFVEVKKSTTGRNTKASHLAYIGDASIGQNVNFGCGAITVNYDGKNK
                     YKTLIGDDVFIGCNANLIAPINIEDNSLIAAGSTLNKNVPKGSLAIARAYQVNKINYI
                     KDKQDKKDKE"
     gene            complement(9379..10683)
                     /gene="glmM"
                     /locus_tag="PLP02_02665"
     CDS             complement(9379..10683)
                     /gene="glmM"
                     /locus_tag="PLP02_02665"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPF42388.1"
                     /translation="MPKYFGTDGIRGKSYEFLDENLARAIGRSLHVLKKSDVIVARDT
                     RESGQMLSQALIEGALEAGFDTIDIGVMPTPALCHISRTREAIGVMITASHNPYQDNG
                     IKIFVSGRKLTLKEEMAIENNIDAVSLVSEMFFGKKKSLEDDPMDIYLNLFRKMIVRV
                     PLSIGLDLANGATCKTAPVVFPRFSDRIFYIGNNPDGRNINDNVGSTHPESLSRTVEK
                     NKLDIGFAFDGDGDRVIMVGNDGLIYDGDMMIYIFACYLKERSLLKKNTVVLTKMSNL
                     GIIKALESKDINVVLTDIGDKYVMEEMDKEGYILGGENSGHIINRLLLDTGDGVLNAT
                     FMLKILHDTETTVSNLTKDVVLYPDKLKNLRNIDPQLTKDPDIVRFIEKRKEELGTDG
                     RILVRPSGTEPVIRIAVSAKTMDEVDEIIESITNVFMNKTES"
     gene            complement(10695..12494)
                     /gene="glmS"
                     /locus_tag="PLP02_02670"
     CDS             complement(10695..12494)
                     /gene="glmS"
                     /locus_tag="PLP02_02670"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPF42389.1"
                     /translation="MCGIVGYIGNDKASDIILSGLEKLEYRGYDSCGIFLYDTLNAKY
                     VTYKDKGRISHLENDFSYEFENHLGIGHTRWATHGKPNQQNSHPHFSASKRFGIVHNG
                     VIENYRELMAKYLDGVVFLSETDTEVIINMVEYFVSQMTVEEAIRKTLSLLEGSYAML
                     IIDKLEPDTLYAAKNKSPLIIGPGHDGITIASDVMALVGYADTYMPIEDKNFVRIDKT
                     PCCYTYQITDIIGKPVELPVFKMDIVLEEIGKAGYPHYMLKEICEEPSVIRKIIGRYL
                     KEGNVSLPENALALFKKAKRIHILAAGTSYHAGFVGKYYFEHMAGIPTEVFVASEYAY
                     NQPLIVDDPLFLFISQSGETADLRVCLQNIKSRGYKAVVLTNVPTSTLARESDASIEI
                     NAGPEIAVASTKAYVGQVAILAIIAYALASKKPFDLVNEMSRVAIAMDAVIDGREYIQ
                     KIVGEKLNKRNCFYIGKGLDYYTCLEAALKLKEISYIQTEGFAAGELKHGTIALIEDG
                     VPVFAIISQKSIQNNTRASVHEVEARGANVITIVMNDVSDSKDDIILMDVEEMLAPLV
                     TVIPTQLIAYYSALNLGRDIDKPRNLAKSVTVE"
     gene            complement(12606..13337)
                     /locus_tag="PLP02_02675"
     CDS             complement(12606..13337)
                     /locus_tag="PLP02_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42390.1"
                     /translation="MAQIKMHLNLLLSRKVILIFIGFQVLLFLGITYASGIIQGTSGI
                     DMMRDEYYVSYLSDTLLQIKFLCALSAVLMASEAASKEYSLYGWYILRKRRDKAFFWL
                     YKSIAVWLIVSVFVSLSVLLLSCGAKIYFKENVSLEFIKESMLSLFVFSVYLSVISDL
                     SMIIFKHRLSIVFPLAFFWFMEANLYQITDSMASGLTEVFAYIFPFVMLKNGQIVLYP
                     DVGGVLLIMFLISVSGCFLFYHNDL"
     gene            complement(13324..13956)
                     /locus_tag="PLP02_02680"
     CDS             complement(13324..13956)
                     /locus_tag="PLP02_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42391.1"
                     /translation="MNASPELIITIDDLKKVYRHNTGILGISTFFSPGLLNLLIGGNG
                     SGKTTLLKCIMGLVRYEGKIHKSKGVIGYAPEKYVMPEFMTINDFLVSIGRIRRDHEK
                     RIPEYLQEYCDLLDLSNVMDKPIGKLSNGMKQKVNLLQAFIGKPRIYLFDEPLSSLDG
                     KMKKVIVKIMFRLCRENIVIVSTHYPECFRSHTKRIYEIESGVLLRDGSN"
     gene            complement(13919..14851)
                     /locus_tag="PLP02_02685"
     CDS             complement(13919..14851)
                     /locus_tag="PLP02_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42392.1"
                     /translation="MKKWFAFVIGLYVLTMAAILIIPGKQVDTISIITVINHESRLID
                     DDDSESFDMYLTKTDSFLTDNECISEAYMESNQDVIRVNLTGIEDLEETVLWNNEEYH
                     RFQVKYGFDSVGLEGVMAFPDACFHIVYKNGDEISLSIGDLSFLFCDLDTEGCLDFFS
                     VYGIYGEYEEKTYLSGICIGLEIASLSDVIIDDVSIGSLKTKIKTGEYAYSTNRFDYT
                     QDMSELLSITSYDPEMSYRLDTKITLEEDGYLLFPIQYEEGIFMLRRFYISIKYEYMG
                     EEYNLLIDDMPYESMPAFSDIEDECIARTDYNYR"
     gene            complement(14848..15483)
                     /locus_tag="PLP02_02690"
     CDS             complement(14848..15483)
                     /locus_tag="PLP02_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42393.1"
                     /translation="MKKGFYFLLLISLFLPLLSTNAKAEEYLNYQEIVFDDDEAMLLK
                     DFSTTNYATYYTKITKKCFSGWKLYVVNENENAEFVSETKLKIYNDGYSTIKHDITLK
                     TSEETKFQVSASGSISTSVDGNIKKFKGSIDADIKASVTYTKTTTTSETYEFTIIVDP
                     KTYVTIVTRGVGEVSNGVAKKYFCWIETKKGGWEIFTVKTEYYEIVKEKIR"
     gene            complement(15528..16352)
                     /locus_tag="PLP02_02695"
     CDS             complement(15528..16352)
                     /locus_tag="PLP02_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPF42394.1"
                     /translation="MSKIGLCLSGGGARGPYQVGAVKALEEMGIMDKITCYAGTSIGS
                     VNSCLVSSGGSAYALEAWNSVNSEIVVQTEHFISLLIKERSNIVEKGCFQIDAFQKAL
                     MKVLQPEKIRGKHVYATISDGGAVSTGFFGLMRANYEHFIKLDSKVAYVPLDNETDEE
                     IRKTVMASCSIPFVFPPVRIDGHNYYDGGLYDNIPLTPLVANGCDKAIVVNLNRFDYL
                     ERDKYPELKIIEIKHSRLLGSVLKFDSERCRQLYELGYEETKKTIDPLILSDFLEE"
     gene            complement(16353..17480)
                     /gene="mnmA"
                     /locus_tag="PLP02_02700"
     CDS             complement(16353..17480)
                     /gene="mnmA"
                     /locus_tag="PLP02_02700"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPF42395.1"
                     /translation="MKIIIGLSGGVDSSVAALLLKRQGYEVEGLFMRNWDSLINSETI
                     GAPTTKEGICPQEKDYLDALTVAKKLGIRLHRHDFVDRYWDDVFQYFLKELDYGRTPN
                     PDILCNKYIKFSAFLDVAKALGADKIAMGHYARVTENGVLLRGVDPNKDQSYFLAELS
                     RDELLQTMFPIGNLTKKEVREIAKKEGFSVAEKKGSTGICFIGERNFPAFIGNYLKPR
                     EGRILSSDKNILGRHRGIANFTIGQRKGLGIGGCNIYEGSPWFVYAKDVKNNDIYVCQ
                     GFYNDLLMSDRCLVTDFNWIGGVMPDDINNLSAKFRYRQKDVAVNLSKAPDNSVMVMY
                     PEKSRAVTPGQECVIYRNDECLGGGTIDKVFLEDNPRTVVI"
     gene            complement(17487..19343)
                     /locus_tag="PLP02_02705"
     CDS             complement(17487..19343)
                     /locus_tag="PLP02_02705"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPF42396.1"
                     /translation="MIGKGFVKICLATPKLAVGNPDYNVSEMLSILKDNPSSLTVFPE
                     LGISGYTCGDMFFHKALFDDVEKALGRFLKENRYPGLVICGLPLVVEEMVLNVAIVIQ
                     KDRILGIVPKYYLPSTREYYEKRWFKSGFDVVEHITKTDIMGQVVPFGNLIFSFGNVR
                     FGIEICEDMWATITPGNLMSVNGANLIINISASNETLGKEEIRRSAVKEHSRKNCGIY
                     LYCSAGASESSSEMVFSGHDIVAMNGHIVAETEEFSLNTIKLFADLDIDRITNDRRNN
                     SSFRDSILKYRYQFQNVPVTLATSDDFIFSRPLEKFPFVPKDDIEGDFRKIASIQEYA
                     LAKRLDHLKIKHLLVGVSGGLDSALALLVACRAFDTLKLDRKGILALTLPALHTSGRT
                     NKNAKKLMESLGVSVKEIDLSESVKKHLELIRNDSTTENTTYENAQARARTMVLMDLA
                     NEIDGIVLGTGDLSEIALGWSTYNGDQMSMYNVNAGIPKTVVSFMIKEYADLVFSEDV
                     SLCLYDILATPITPELKSDQTTESVIGKYEINDFILYRFLSCGDGADRIVFLLEKTFD
                     MNQAECETYVSNFFKRFFSQQFKRQASPDSPKVLDVALSPRTDFRLPSDMDR"
     gene            complement(19348..19923)
                     /gene="nadD"
                     /locus_tag="PLP02_02710"
     CDS             complement(19348..19923)
                     /gene="nadD"
                     /locus_tag="PLP02_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPF42397.1"
                     /translation="MIVIFGGAFNPPTKAHIAIYRRVRETIDCEKFIFLPVSSLYTKR
                     SLASNQHRFEMLSLLVGRDKVAEVSPMELEDADYLGTYQSMLRFQERYPDREIVFLIG
                     ADNLLKLHKWINAKSLLAEFRFIVMNRDKRDIDKLISDDPFLRDYLDRFIIIRDFHME
                     MSSTGFRDSFDREMLTDEVYDYIETQGLYRG"
     gene            20045..21067
                     /locus_tag="PLP02_02715"
     CDS             20045..21067
                     /locus_tag="PLP02_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPF42398.1"
                     /translation="MKETLDYIFDLAKKILSIPSPSGYCREVIDFVSNEAEKMGFKVE
                     RTVKGNALIKVKGTGNLTLGLSAHVDTLGAMVKSITSDGYLRFTTIGGPIWPTLDGEY
                     VMIRTRTGKKYDGTFLSTSPSVHVYKDAMSKERTPDSMMVRIDETVHSKDDVIKLGIA
                     PGDFIFIDPKTTITDSGYIKSRFLDDKISVAILYGLLKEISENRVKPKMNLEIIISTY
                     EEVGHGAASLPHVDELIAVDMGCVGDDLSCTEEMVSICCKDSTGPYDYDVVTRLVESA
                     KVIKADYATDIYPFYGSDVSSALRAGHDVKGGLIGPGVHASHGMERTHRKGIENTLKL
                     LISYIS"
     gene            21163..21465
                     /locus_tag="PLP02_02720"
     CDS             21163..21465
                     /locus_tag="PLP02_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42399.1"
                     /translation="MKQFFNVLGQLFGFLTILLFAFIFANELFGFGVSADIINILETV
                     KVYAVFGVCGLAGFEMVAGKKFFAFLYFIILAFVVIMSFFPSVGNLIRSYVTTFIA"
     gene            complement(21497..22375)
                     /locus_tag="PLP02_02725"
     CDS             complement(21497..22375)
                     /locus_tag="PLP02_02725"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF42400.1"
                     /translation="MDLIYRAKKPETIKRFLLENNIPLKLVKSEEGKMMLFVNNDLKT
                     KDDTMKKGDTIKIRIQDEKPESDIIPEDIPLDILFEDEYFLVVNKPSDMQVMISKTHP
                     TGTLANALAFYYKKNGLGSQIHFINRLDKETSGLMLVAKNRFLKFLFSDRTDNSITNE
                     YLAVVQGLMDIKRFCIELPISRSEGSIKREVSETGDECKTSYQVEREFDGFSLVRILS
                     ETGRTHQIRVHFSYFNYPIVGDLLYNKNRYPVKDMLLYSNLIRFRHPIKDQDVEVRLP
                     ASEEIRAFMKMHGALI"
     gene            complement(22449..23558)
                     /locus_tag="PLP02_02730"
     CDS             complement(22449..23558)
                     /locus_tag="PLP02_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPF42401.1"
                     /translation="MKDKRIEKLAQMLVNYSISMKEGENVLIKAPVKAKPLILALVRA
                     IREGKGNAIVELSDPDIGREVMLGMSDKWLDRSYRWMDAKLDDLDCIISIRASESDYT
                     DVDVPMETKLKIAKKLNPLSEKNLSKKWVLLNYPTEGAAHKARMSYDAYFDYVIGVSA
                     IDYSVMNKAFEPLKNLMEKTDRVRLVAKGTDLSFSIKGMNAIPCAGNNNIPDGEIFTA
                     PVRNSVNGTITYNTPSPQRGVVFTGVSLTFENGKIIKAVADQENDLLEQIFNTDEGAR
                     YVGEFAIGVNPLVTRPMGDILFDEKIAGSIHFTPGRCYTEAPNGNDSAIHWDLVQIQR
                     PEYGGGEIYFDDILIRKDGMFVIPELLGLNPENLK"
     gene            complement(23555..24094)
                     /locus_tag="PLP02_02735"
     CDS             complement(23555..24094)
                     /locus_tag="PLP02_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HPF42402.1"
                     /translation="MEYIPGTKIKILSFKHDGTLHRIWEESVVVKNEDEWMVVANRKT
                     KVIEGNGRFWFTKEPCVSFFSPLHWYNVIGIIRSSGISFYCNLSSPLLTDEEGIKYID
                     YDLDIKVMPDLSYTILDKNEYRRHSEEMSYSEELKKILETEFEDLEKRIKNREFPFSY
                     SIVKSEYAAFKKIGAKNKC"
     gene            complement(24181..24699)
                     /locus_tag="PLP02_02740"
     CDS             complement(24181..24699)
                     /locus_tag="PLP02_02740"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HPF42403.1"
                     /translation="MKINIVLYNPEIPQNTGNIMRTSVGTGAVLHLIEPFGFKFDLSI
                     LKRSGVNYLEHLEYHIYKDWQDFKIKNPGRYFFLTRYGRKSPDQCDFTKAGESIYLIF
                     GKESTGIPKAILREHLDDCLRLPMTDKVRSLNLANTVSIAVYEVLRQLGYPGLFREEP
                     ESLKGACWLEKE"
     gene            complement(24768..26033)
                     /locus_tag="PLP02_02745"
     CDS             complement(24768..26033)
                     /locus_tag="PLP02_02745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF42404.1"
                     /translation="MKFSKPYLNDALASLGFDELTTIQKSVLEKAASKKSLVVESNTG
                     TGKTHAYLLSIFERICEDADSVQAVIVVPTADLAKQTYKFAEKLAEFSKPKVNVMLFS
                     GGSDRERDIASIKKNHPQIVVGTPGRLADLIRNENALVINKTLFFIIDEADMALDGGF
                     LEDVDKIASSVGKDTQFMIFSATIPENIKPFLKKYIGNTEFLKDEINKENASLIQHVF
                     VKTETDERMKKLDEVTLAINPYLAIVFCNTKKTAESVNQQLKDSGKSTVLFSGDVEFR
                     KRRQIIDRIRRLEFQYVVSTDILSRGIDIESVSHVINYELPKETGFYIHRSGRTGRMG
                     SDGVCVSLYTKEEMPLIEKLEAKGLKCDFRYVSDRELLPARRQNERNERKATDQKKTV
                     RPKNLKVKPNYKAKFKQNSGIKQSSKIKK"
     gene            26118..26996
                     /gene="ispH"
                     /locus_tag="PLP02_02750"
     CDS             26118..26996
                     /gene="ispH"
                     /locus_tag="PLP02_02750"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPF42405.1"
                     /translation="MEVLTINPRGFCHGVIRAVEIVERAVLDRSVPKPIHVLGMIVHN
                     RHVVDDLSLLSVITLYPKSGNYSELLDSINTGTVVITAHGIDERIIKKAKDKGLYVID
                     ATCPFVHKSKHHLAKWLSKGYVVAYIGKQGHPEALSALSLSDDIFLIENGQDVDKLPE
                     NTQKLYVTTQTTCSQNDVSSIMETIAKKYPNAIFEDEICNATARRQDALIAANKEIDL
                     CVVVGDPSSNNTRNLFKISKEITNTRTIMVNDVSEIDPSFLKDVKRISVTSGASTPDR
                     VTDSIIAFLRSYDQHD"
     gene            27082..27252
                     /locus_tag="PLP02_02755"
     CDS             27082..27252
                     /locus_tag="PLP02_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42406.1"
                     /translation="MANNKKKPNITSSEEKEIRTYNPTKSHFGKIIIVILALGMFLAM
                     LIAAILGMIEVL"
     gene            complement(27309..28979)
                     /gene="recN"
                     /locus_tag="PLP02_02760"
     CDS             complement(27309..28979)
                     /gene="recN"
                     /locus_tag="PLP02_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPF42407.1"
                     /translation="MLEYLLVENFAIIDRVEIGFKPGMTVLTGETGAGKSILIDAISL
                     LLGDRASQDMIRSGCESAKVTGRFRIKNADTKRQIIAMGISMEDETIEISREITNSNR
                     NMSRINNVSVTLQQLKAVSIRLADIHSQFDTQRLINPQNYLALVDGYRREAIGTYLEK
                     YRQDLAGFQKTVRDLSLLLEETRKYEEKRDLYIFQLNELTEAGLDPEEKRELQESRTL
                     MKNFDKVYESLAEIKQRFDDEPVLEGLAGILENLDRLSDYSEEYKNLRERMADHYYEI
                     KDIAEECILKLDQLNFDPNSLDKIETRLYLLDKLEEKYKMTIPELCEYRDRLEKLSGG
                     SFERMEEIRDLERKKEEEKKSLIQSAGDLSKLRKDAAKQITAELGILFGDLSLANTRF
                     EIRFADIPDNPDNFGQDGIDSIDFFISTNPGEPLRELSKTASGGEMSRVMLAFKTIFI
                     RTEHLETMVFDEIDTGISGVVASQIARKIREISENCQVLSITHIPQVVAVAANHLKVE
                     KKITGNRTTASAGYLDFDNRVKEIAMMISGDKVTENALENARELLLEN"
     gene            complement(28998..30854)
                     /gene="dxs"
                     /locus_tag="PLP02_02765"
     CDS             complement(28998..30854)
                     /gene="dxs"
                     /locus_tag="PLP02_02765"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPF42408.1"
                     /translation="MTDLYEIKDPGFLKEMNTKELNELAFSIRSFLIDSISHTGGHLS
                     SNLGVVELTIAIHKVFDSPRDKIIFDIGHQGYTHKILTGRASDFATLRKHGGLSGYLK
                     HSESVHDCWEAGHSSTAIAALAGFEIARMRDNKDYKCLALVGDGSLNSGLSFEALNFI
                     GHRPGLHPIIILNDNEMSISKNVGSLARLLNSMRTNKAYQKARMIKNVPAFMIDLKNR
                     VARMLRGFAKNMTIFDEFGLSYYGPIDGHDFKTLTKYLNLVKNAKKPCVLHVVTKKGK
                     GYPFAENDIAGSWHGVDPFYVETGLPRTKKAENRTSWSNIVADHLFMKAKNGDKFEII
                     VPAMVNGSGLKRFQEAFPSMITDVGICESLSVCLAGALSIGGRNVFVPIYSSFLQRAY
                     DQVIHDVARQNLHVIFGIDRAGLVEADGETHQGVYDIAYLSHIPNIEIIQPKDSSEAR
                     SLIDYAFEIAKHPVAIRYPKADTDKRNDEICEPILEPSWTVSNIEAENAIITYGDTVT
                     RIENIINRKGFDYAVVNARFIKPIDETTFVSVMENKKNIIVIEENAKIGGLGSLILEK
                     AVELKLDASKISIVALPDEFIGIGTREELENDYSLDDASIVQLLKSSEKPEM"
     gene            complement(30868..31089)
                     /gene="xseB"
                     /locus_tag="PLP02_02770"
     CDS             complement(30868..31089)
                     /gene="xseB"
                     /locus_tag="PLP02_02770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPF42409.1"
                     /translation="METKMTFETALSKLEEIVRELEKGDLPLDKSLEKYQEGMRLAKF
                     CHEEIQNAENVIVKLMKDDKLEDFEEPKE"
     gene            complement(31094..32365)
                     /gene="xseA"
                     /locus_tag="PLP02_02775"
     CDS             complement(31094..32365)
                     /gene="xseA"
                     /locus_tag="PLP02_02775"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPF42410.1"
                     /translation="MEKKYITVSALNRYLKYFFDNDKNLQNILLKAEISNFKRHSRGH
                     LYFTLKDDSSQIPAVMFQSNAVSLDFEPKDGSKVVVEGYVSVYEPIGNYQVYVRKMYE
                     SGIGDLYQAYENLKKKLSEAGMFDESHKRPLPQYPKNIGVITSPTGAAVRDIIHIVNR
                     RYPLARIIVYPTLVQGDEAKYSIVRMIEKANKDLLSDVLIVGRGGGSIEDLWAFNEEI
                     VAKAIYESKIPVISAVGHETDFTIADFVSDRRAPTPSGAAEIVVPDQNTLLMQISGYT
                     RNMAVSWQKYIQVKTDMLNTLRRSPVILNGERLLEKPELKLASAYDRLLQKRPDRILD
                     SYREKTNNLKTRISLVLENLRLALETRVENDIDRLELVNPLSLMKKGYAMARKNGTIV
                     KTVSLLEKGDNIELQMSDGIVGCTVDRIGKD"
     gene            complement(32369..32758)
                     /locus_tag="PLP02_02780"
     CDS             complement(32369..32758)
                     /locus_tag="PLP02_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPF42411.1"
                     /translation="MNKEQARKKREDIINLIYATHMGGGFDKSQYDENVIATFAKVEE
                     KMPDLDDIISRNLTNWTIDRLNYVDLAIIRYAVYEMKYADLPFEIAINEALELTKKYT
                     NLDDNLAKSFNNRLLDNIKTDLFGTKR"
     gene            complement(32852..34549)
                     /locus_tag="PLP02_02785"
     CDS             complement(32852..34549)
                     /locus_tag="PLP02_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPF42412.1"
                     /translation="MQEKWWMKENIYQIYLRSFYDTNGDGIGDLAGVAEKLPYLAKLG
                     IGVIWISPHYDSPMDDNGYDVRDFYRISDDYGDLDDFRILIEKAHGLSIKVITDLVLN
                     HTSDENPWFVAARDPSNPEYEKYHDYYIWQKPKKGPTGERTLPTRWISWFGGPVWDYN
                     EATDEYYLHIFSKKMPDLNWRNPEVHLEMKRVIRFWIGMGVDGFRVDASNHLEKDWEF
                     KDDYPGYNHFSSLPKHHEYLRELGRELFVPNNLLVIGESGGASQKEALQYVGFGSDEF
                     NLLIQFGHCWADTDENNPRLMGKWSEGKLQVHKIKESFDRWYEMLDEMGWNVIYWHNH
                     DQPRIVSHYGSDEPKWRVKSAKMLAIALYFLPGTSICYQGEEIGMTNVDFNDISLFRD
                     VEVFTEYRNFLNRGYCEEEALGIIKRRCRDNARTPMQWTAGKNAGFSQSEPWIKVNGN
                     HETINVENQDADPDSVLNLYRMILNQRKTDSDIYAGKACFIDKGSDESFVYENKGKNN
                     SLLVICNFTKEKTAIGLPKSILKGYMPFLKNTDTFPVLSGLVELDPYDAVVYRKKNR"
     gene            complement(34568..>35924)
                     /locus_tag="PLP02_02790"
     CDS             complement(34568..>35924)
                     /locus_tag="PLP02_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42413.1"
                     /translation="KRLFPDDFMVTEKMTLKYNVSGNVRTLQFINKTSSLAIECLVKD
                     MTDKLIQDTENKEASFSGEYYMMPGMSGLAKANNGPFKQKSYLVVNFQNMDEVYSLPD
                     ETVPARLAVRFKDMAEKSARSRLLGLYALSSSSFVLLLETNDKRMIERIARELRSIDY
                     QEILYPVMQISTNALVINRPTDFKVIRRQLLEMSVSRNEEENVLYTSRQRSFSDELSV
                     SILEATNRILAEGALSLEYHPIGRWQEKKIFWLEAALNSRSLFGSKEQLDLALVGFNK
                     VREYQNAFSKRFVKDVKYFLKKNDSGTVFLYPIEKYLLMRPEMISYLIRETNKAKIDP
                     QRIIFMIKSPFSSRKKLAEVKKSITEGGYSFAFSEWLNDATGNDLPLMREAVFGVISS
                     EEFDKTVPEWYHVCQQAYSHGIVFDHQARPLKKSFLSENKIEYVMGEYYPAIDIRQLA
                     D"
BASE COUNT         9202 a         8142 c         7507 g        11073 t
ORIGIN      
        1 taaaggagat atgtaaatct tgaatttctg gctagcagga atgaactctg tcaaaacatc
       61 gttcaaagca tggattcctt cgataatgat gggattatga ctctcaatat gaatcggcgg
      121 agtgaaactc cgcatatgct ttcgaaagtc gaaaaacggt aaactgacgg aatctccgtt
      181 gattaggtct gtaatattgc ggttgaacag ttccaaatca agcgctttga catgttcaag
      241 atcaggctct ccgaattcgt caagcggtgc tttgtcgaca ggaagatagt agttgtcgat
      301 cgatatcatc aaaggtttga tccctctcgt caacagctcg atttccaagc gtcttgaaaa
      361 tgtcgttttg ccggatgatg atggaccggc aatcgcaata aggcggatat tgtcaatatc
      421 tttagaaatg atttcgccta actcgcacag ttggttgtta tgctttgtct cgcacatgtt
      481 cacaaactcg acctgtcggt tctcttcgat ttttcggtta agcttataga tcatgtcggc
      541 atcacagttg attgcccatt gctcggctcg gttcagaacc cgcaagaaac tgggagaatc
      601 ggaaaactct ggaatttggc ctttttcttc gatccgcggg tatctgacca aaaatcccgg
      661 actatagtaa aagagttcga aatacgtcag atatcccgta gaagggacca tatagccgta
      721 catgtagttg caatatccgt cgcattcgta aatattgaca tcttccttgc ggtatttcaa
      781 ggtttctact ttatcaatat atcccttgct ccgataatat tttttcgcct cggaaatcga
      841 tatttttttt cttgtgatcg gataattggc tttgatgata ttctgcattt cagttaaaac
      901 cagctgaatc atatccggag tgatgattcc gttaatggct cttccgtaga ttcccatggc
      961 gatgctgttc gagaatttta tctgtagatt tggaaaaaca cgtctgaatg ccatgcagat
     1021 aagataacgc atgcttgtgg catatatcct ggccgtgtcg gaataggtat agtcaagaag
     1081 atcgacatcg gcatcaaagc cgatgacata tgtgagttcg cgcaagcgat tgttcacctt
     1141 ggcggcataa tatgtccctt cgtatctgga cgccagttcg gtcaacctga ccttgttttc
     1201 aaatttcagg gtttcaccct ttatattgat tgtaaacaaa taaatcactc ccatgaaagc
     1261 gttagcatta actattctac tacttttaca aggaaatgca agcactttgt caaatcaata
     1321 aattttgatg caaagctcgt attgttgtgg tataataata acataaatcc ggaaaggggt
     1381 aatataatgc tactgtcgac ttttttctcg agtaccggaa tggatgaaag tccaacggcc
     1441 tatcttttta aatggccaca ctttctttac atgtttctgg tcataaccgg attcatcgta
     1501 ctcatgcaga tatttgttca taaaagcgat aaaaccagac atattttttt aatagtgatc
     1561 gccgtcatca tgcttttgct gaaatattct ggtgaagtct tatatatatg ggaatggaac
     1621 cattacggcg atacgatttc cagttcctcg catcctttct gggatgtcag gacattcatt
     1681 tccttccagc tttgcggcat caacaacgtt ttgctgccgc tcgtgataat cttcaattta
     1741 aaaccgatgc gagacttcat atatacgacc tcgataatcg gcgggctggc cgccatgttg
     1801 tatccggtgg ggattttatt tggcgacccg ttcgtcatta cgtttccgat gatccgaacc
     1861 ctcgtcgttc atttcctgct tttgttcact ccttgcttca tgatcgcgtc cggagattat
     1921 acccttaagc tgaaaagttg gtggaaaaca attcccatcg gatgcattgc aatgtatctt
     1981 tgggcgatgt tcggaaattt ggtcatcgat cccggcgcca ataatatgta cctgatggaa
     2041 aatccgttca tcggcgtcgg tattcccata ttaagcgata ttccctcagg cctccatgtt
     2101 ctcttgcttc tggcaatggt ttttcttggt tttttcattg tttatatgtc aattggtgca
     2161 tatcaaaaac atcgtgataa aaaaatcgca atataataaa agtcccggac aaacaaattc
     2221 cgggactttt atttttaatt atcgatattt actccgacaa gtacaatgcc agcaactccg
     2281 aaagatccat gttcagcaat tcctgaaaac cggtttcagt ctggtaatcc gaaacagtgg
     2341 catatatgat cagaatcaat tccatctttg cttccgtaat cccgtatgtg gcagacagcg
     2401 tctctaaatt tatcgttcct gagaaggcat agttaggcgt gtaagtattg tagctgtcgc
     2461 ttgacgtatc gtttcctgta gtcgtggaaa tggctgcggt agtcgttgtg gttgtcgcct
     2521 gttcctcgga agcgcaacga gaagacccga aaagcgacca gatatcataa tcggaagtca
     2581 aagaattcgt aaccgtgata ctatatccgg aagccataag atcgcctgtc atcaagtgag
     2641 tctttataat cagatatgaa gatgtgccgg ctttctcgca tcggctgatt atttcgctga
     2701 ttccatcctc aatttctggt ttagttcccg aaactccgaa taacaaagcg gtatcgtcta
     2761 tgtctgaata tcccaaagct tcgcttgtct gtaaaatgtc tgaaagaaca accgacagtg
     2821 aaccgctgtg atatgtgaga ttttccacta aaatctctcc gtcgctgttg agcggaatca
     2881 ctttgcgaac gcgtctgaag gaattgattt ccaattcaac cgacgggttg atatcgacag
     2941 tcacggtcgc agtgatttta aaatcaagat aaagtccaga tcccaaaaga acgacaaaca
     3001 aggcaagaga aaaaccgacc atcaagcgat gccagagcgg ctgtttccta tcgtttgaca
     3061 gaacgggtat gtaatgtgat ttcgcttctt cgagaaaaga acggttttcc ggaagcttga
     3121 atgcggcgtt atccttgata atttctttta gttcgcgata tttcatgatt catcgctcct
     3181 tgaatttggc cttcagtttc ttcaaggcct tggcgtggat ccatgtaacg gtcccgatcg
     3241 gtttgtcgag tatgacagct atctcgcggt gaggaagatt ttccagattg tgaagaagaa
     3301 agacattctt ctcgtcaaga tctaatgaat cgagtatttc cttgacaagc tcttcttttt
     3361 ccgcttttat ttccagatgg tccgcgagtg agacttggga ataatcggct tcgactgatt
     3421 ctatacggat ctcccgtttt cttcggttgt attcgttttt cgccaggttt ctggcgatgg
     3481 taatcacata ggctaataga tttctttccc ggtatcccga aagacacgcg catagtttca
     3541 tgtatgtgtc ctgaacgatg tcctcggcaa gcgagcggtc gtttaagata ggcaaaacgg
     3601 cgaaatagac ctgacggtat gtcaattcat acaattggtc aaatgcttcc gtatcgcctg
     3661 cattgtattt cctgaccagt tggtccagat cgtctggcat agtctcacct cgttccttga
     3721 gactatttta tcatctttcc agacaaatcc ggcgcttttt ttgcttttca catatataaa
     3781 cattcatggg atgcgatttg ttgcaaaaaa aagacgaact gtcgagaatt cgtctttatt
     3841 ttcttatctt gccatccata ttatgaacga catagctgga attttgggtt atcgctttga
     3901 tggtggcaaa agctatcgct tcgcgctggg taggcagaac tctcaatatc cggtccgatc
     3961 cctcccgttt tacataccat gaaccatctt cctttcggta gtaaacatga tatctgccaa
     4021 gttccttgtg gactgcggcc ttttttggag ttttcttttc ttcaggcttt tttattcctt
     4081 tttcgtcagg aacctccggt ttgtcttctg cgttttgaat gacttccgga attattttcg
     4141 gctttttttc ggctatttct tctacaatga tcggtttcga cacttccgga ctgattattt
     4201 tttgcgtgaa aatggtttgt tccggataga tatctttttt ttccaaagcg ctcggttctt
     4261 tggcgtttgg aacgatcacg tcctggtcga tgagatcttt gagcgaaata gtcctttcgt
     4321 gcgtgtctgc ttcttttggc agttgttttt ccatacgcgc aagtttcttc cggctgtcaa
     4381 ggttataaaa cagatatatg atcgtcatca atgctatcgt tccgaaaacg atggcaaaga
     4441 aacgccagta ttccataaag aaatccaaca gcatcttgtt tccccctatc ctttcttaaa
     4501 acaatttcat ttgcatgtcg cttgtgggct tgaccgatct aatgatttcc ttcataccaa
     4561 aatcaacatg cattttcttg gcggaaaata caaacgcttt tttcaacttt tcggcgtttg
     4621 cggaactgat cgatttacga attccgaaat tatccatgta tatgtttttg agtccagggt
     4681 agtgtttgtc gatcaggcta taaaaactcg gacgttgttc atcgcttatg atgattccaa
     4741 aagatggata cataaacgat gctccagctt ccttggcttt gtttaaaatc tgaagaatat
     4801 tttcctcgga atcgttcaca aaggggatta cgggtttcaa gatgactcct acccggagat
     4861 tttcgcttgc aaaacggcgt atcagtctga gtttagctgc aaacgatggt tcgctgggtt
     4921 cgaacattct tcccatctca tcattgacaa aaccgaccgg tattgcgatc agtaccggag
     4981 cgttctcact gattttgaca ataatgggaa tatcttctag aacattttcg gagctcgttt
     5041 ccagataaag tcccatgttg ctttccttga ttgcttcgag aacctttttc atcgtcccga
     5101 ctttatttat ggaggcgaca taaggatcgg gattttgatc gaatccgatt accgatccct
     5161 ccggtatcct tctcatttcg tagataaatg actccatccc catgtgtttg atatcttcta
     5221 gttccggccg gtatgtctgg tcgatgtcag ccggggatgc gtaaaatttt cttgtcggat
     5281 ttgacccctg aaaaatcgag gcaaagaaat caacaccgaa gtagcgctga gggttcttgc
     5341 acgggaatac tgtattttga aaactagaag ctgacattgt ggcctcctaa aagcgccaga
     5401 tcgaagccat caggatgaga gcaaatatga tattgaagta ttggaaatat ccgaaatgga
     5461 ttggaacctg gctgatttcc ttgtcaagtt tcttgacaaa tgccttgtgt tcgtccttgg
     5521 tgagatcttt ggttattatg atatatgaca gattgcggta attatatatc tttccttccg
     5581 gaagcgtttc ggaagcattt ccgagcgcga ttttcagatt atcggcttcg cgttttgaaa
     5641 agctgtatgt tagacgaata tccgtttttc tatatgcgag atagatgcgc attgcagcgt
     5701 aaataatcag gattccgcct ataattgaca catatgcgac aatcaggtaa tgaccgataa
     5761 aaaagaaact gacgatcatc cacgaaaata cgagaaagaa atccgtgacg gaagaattgg
     5821 tatggtatct aaaaatggtg ttgaaggaga cgtaaaggat gaagatagtg catattccct
     5881 tgagaaagag ttcgaaatag gcttccatta atagtttcct ttccttgtca agaaagattc
     5941 agtctttttt cgactgcatc taccagcgta tcgatatccg ccggttcggt gattctcacg
     6001 acggggactt tgacatcggc taaccacatt tcatgttgtt ttttaccccg caaggattcc
     6061 ggagtttcgt cataaaggct gatccactcc agaaaatcaa tgtgtttttg gtaaagatcg
     6121 cctcctggga ggacccggtt accaaaacgc gccttctcac ggctttcaat ccgacttttc
     6181 cggatttcta acggaaggtt aagatagaca aagagatcga tcttttcctt gatatcgtcg
     6241 gcccagccga tgaaagaacc ggagatcacg attttgtcgc cggttttcat ccgtgcttcg
     6301 agatattctc tggcgtcctt gtcactttta cgcttggaaa aaggcaattc ggttttttcc
     6361 cagatcgcat catcggtgtc aatgaaaata tatccgtatt tttttgcgac ggctctagcc
     6421 aaagtggtcg atcccgaacc ggatgaaccg aagatattga ttgttttgat tttgcccata
     6481 aataccaccc tgcaaaaaag accgttttgt atccaattac aattatatat taacaaaaac
     6541 atatatattt atcaatgaaa aacccgttat ttgccggctt aaaaaataaa aaaacccgta
     6601 ttgtgggaac atcttccgga tacgggttta tcatgacaat aattacttat ctgttttggc
     6661 aagtgctttt ttcgcttggt cgcagacagc gctgaaacct ttttcgtcat tcacggcgag
     6721 atcggccagc atcttgcggt tgatttcgac attcgcaaga ttcaaaccat tgatcagacg
     6781 tgaataagac atattgttca aacgggctgc ggcattgatc ctggttatcc acagtttacg
     6841 gaaatcccgt ttccgtctgc ggcggtcgcg gtatgcgtag gcaagcgagt tcatcacctg
     6901 ctcatttgcg gtcctgtata acagatgctt ggcgccgtaa taacccttcg ccagctttaa
     6961 gatacttttc cgtcttcttc ttgtggtgta tccacctttt actctgggca tgatgtcttc
     7021 ctcctaacct tttacaaata cgggacctga tgtctgattc ttttcagatc agatgtaccg
     7081 acggtctctt ttcttctcgc ctgacgattc tgcttcggcg atttgtggct cattaaatgg
     7141 ttgtggaaag tactcgctct caacaacttt ccggtgccgg ttcttttcag tctcttcttc
     7201 gtgccggcat gagatttcat ttttggcatt tttattctcc ttttggttat tttttctttg
     7261 gtgtaagaac catggtaaga tatcgtccct cgtgggcgat ttctttttcc gtggatgcga
     7321 tttcgtcgca gcgctggcgg aacttttcca agacgtcctt gccttgtctt atgagcatgt
     7381 ctcccgcccg atatggcaac cataccgaaa ctttgagttt gtcgcctttt tcgagaaact
     7441 tgatcccgtt tctcaatttt gtttcatagt cgtgcgtatc gattacagcg gtaagccgga
     7501 tttccttgat gtcgacgatc ctctggtttt tcttggcttc gcgggctttt ttctgctgtt
     7561 cgaacttgaa tttgcggtaa tccatcagtt tgcataccgg tggctttgca gttgcggcga
     7621 caagaacaag atcatatccc tgttcatagg cgatcgaaag ggcgtccctt gtctgtttga
     7681 tgccgtactg tgtcccgtcg gcgccgataa gtagcacctc gcgataacgg atctcctcgt
     7741 tgatcggagt ttcgtctttt tttaccggtt tttgattgat gttgtaaatg atacgtaaca
     7801 cctcctgaaa ataaaagcgg gtgcagaaac cgcacccact tgaaattgac tcaacgtttt
     7861 gtagcgtcta cctattgatc aatcaatcag gtgagaagtg gggcttctgc tttctacttt
     7921 ttatcctgta atagtatata tcaagggaat ctagttgtca agaaataaaa tcattcctta
     7981 tcctttttat cttgtttgtc cttgatatag ttaattttgt tgacctggta tgctctggcg
     8041 attgccaaac tgcctttagg gacgttcttg ttaagcgtcg aaccggcggc aatcaacgag
     8101 ttatcctcga tatttatcgg ggcaatcaga ttggcgttac agccgatgaa cacgtcatca
     8161 ccgatcaatg tcttgtattt gttcttcccg tcgtagttca ctgttattgc gccgcatccg
     8221 aaattgacat tctgtccgat cgaggcgtcc ccgatatatg cgagatgcga agctttggtg
     8281 tttcttcctg tcgtggactt tttgacttcg acgaaattgc cgattcggtt cttcggaccg
     8341 atttcggcgc cgtcgcgcaa atgggcgaaa ggaccgacgg aagtgtcttc gtgaacgatc
     8401 gaattgtaca caagcgaatg acggcaaatc acccgttctc caatgatgct ctcatggatt
     8461 tctgtgtttg gtccgatctg ggcgcctttt ttgatgacgg aattcccggt tatgtaagtg
     8521 ttcgggttga tcaggacgtt ttcctctatg accacattct gtccgatggt gatcgtttcc
     8581 gggttgatca tggcaacgcc actttgcatc aatcgcttgt tgatggaaag gcgcagtttc
     8641 gcctcagcga tcgaaagagc gtatagatcg ttgacaccca tggcttttgc ggaatctttg
     8701 agaaaatagg tcccgatcac ccggttctcg tctttgaata tcttgacgat atcggtaaga
     8761 taatattctt ttttcagatt gtcgttacct attttggcaa gcgatggaaa caacagatcg
     8821 ttcttgacaa cataaattcc cgtattgatc tcattgatcg ttttttgggc gtatgaagcc
     8881 tcgtgttcct caataatcgc ttccaggtat ccggaatcgt ttcgcatgat ccggccgtag
     8941 ccgaaaggat cttcgattct tgttgtcaca acagtgagat catgacgcct ttcgatatgc
     9001 tccgaaatag ccttttcgat cgtctcttca tcgactaaag gcatatcgcc aggaagaatg
     9061 acggtgaaac cgtcaggatc ggtaatgagg catttggcgg ataatacagc atcaccggtt
     9121 cccagttgtt cttcctgcaa ggcatacttt accgcatcct ggagaatatc ctggatcact
     9181 tctcttttgt atccgaccac ggcgacgatt tcatcgacgg atcccgaatt tctgatattt
     9241 tcgatgatat aggcgatcat cggtttgcga agcaacggat atgcgcactt cggcaattcg
     9301 gttttcattc tggtgccttt gccggcagcg agaacaatag cgtatctttt cataccattt
     9361 acctcttatc tatagaagtt agctttctgt tttattcata aagacattgg taatcgattc
     9421 gatgatttca tccacttcat ccatggtttt agccgagacg gctattctta ttaccggttc
     9481 ggtcccagaa ggccttacaa gaattcttcc gtccgtgcca agttcctctt tccttttttc
     9541 gatgaatctg acgatatctg gatccttcgt caattgggga tcgatatttc tcagattttt
     9601 tagcttatcc ggatataata caacatcttt agtgagattc gaaacagtcg tttcggtatc
     9661 atgtagaatt ttcaacatga aagttgcgtt taaaacacca tccccggtat ccagcaatag
     9721 tctgttaatg atatggccgg aattttctcc tccgagaata taaccttctt tgtccatttc
     9781 ttccatgacg tatttgtcgc ctatatcggt cagaacgaca ttgatgtctt ttgactcgag
     9841 cgcctttata atgccaagat tgctcatctt tgtcaaaacg acagtgttct tcttcaacag
     9901 gcttctttct ttcaaataac aggcaaaaat gtatatcatc atatcgccgt catagatcaa
     9961 gccgtcgttt cctaccataa tgacacggtc cccgtctccg tcgaaggcga acccgatatc
    10021 gagtttgttt ttttccaccg ttcgggaaag cgattccgga tgtgtcgatc ccacattgtc
    10081 gttgatgttt cttccgtcgg gattatttcc gatatagaaa atcctatccg aaaatcgcgg
    10141 gaaaacgaca ggtgccgttt tacaggttgc tccgttcgcc agatcgagtc cgatggatag
    10201 aggaactcgg acaatcatct tacgaaaaag atttaggtat atgtccatgg gatcatcttc
    10261 cagtgatttc ttctttccaa aaaacatttc cgacacgagt gagacggcgt cgatattatt
    10321 ctcgatagcc atttcttctt ttagtgtcaa tttgcgtcct gatacgaaga ttttgatacc
    10381 gttgtcttga tagggattgt gactggcggt tatcatcacg ccaatcgctt ccctggtccg
    10441 gctgatatgg caaagcgcgg gagtcggcat gactccgatg tcgatcgtgt caaacccggc
    10501 ttccaaagcg ccttcaatca gggcttgtga taacatttgt ccggattcgc gcgtatccct
    10561 ggcgacgatg acgtcgcttt ttttcaatac gtggaggctt cttccgatgg cacgggctaa
    10621 attctcgtca aggaattcat atgatttccc tctgattcca tctgttccaa agtatttagg
    10681 cattcaatag cctcctattc gacagttacg gatttagcca gattcctcgg tttgtcgata
    10741 tcacgaccga gattcagagc ggaataataa gctatcaatt gggtcggtat gactgtcaca
    10801 aggggcgcca gcatttcttc gacatccatc aaaatgatat cgtctttgct atcggaaacg
    10861 tcattcatca caatcgtgat gacgttcgca ccgcgggctt ccacttcatg gacgcttgct
    10921 cttgtgttgt tctgaatcga tttttgcgat attatcgcga acacggggac tccatcttcg
    10981 atcaaagcta tggttccgtg tttcagttcg ccggcggcga aaccttccgt ctgaatgtag
    11041 gaaatctcct tcagttttaa cgcagcttcc agacaggtat agtaatcgag tccttttccg
    11101 atataaaaac aatttctctt gttaagcttt tctccgacga ttttctgaat atattcgcgt
    11161 ccatcgatca cggcgtccat ggcgatggcg acacgggaca tttcatttac cagatcaaat
    11221 ggctttttgc ttgccagcgc ataagcgatt atggcaagaa tagctacttg accgacatat
    11281 gccttggtgg atgcgacggc aatttccgga ccggcgttta tttcaatcga ggcatctgat
    11341 tcccgtgcca gagtcgatgt cggtacattc gtcaatacca ccgctttata tcctcgtgac
    11401 tttatgttct gaagacaaac ccgtaaatcg gcggtttcac ccgattggga tatgaaaaga
    11461 aacaagggat cgtcgacgat aagaggctgg ttataggcgt attcgctagc cacgaatact
    11521 tccgttggaa ttcctgccat atgttcgaaa taatattttc cgacaaaacc ggcatgatag
    11581 ctcgttccgg cggccaaaat atggattctt ttagcctttt taaaaagcgc aagtgcgttt
    11641 tcgggaagcg atacatttcc ttctttaaga tatcgtccga tgattttgcg gatcaccgaa
    11701 ggctcctcgc agatttcttt cagcatgtag tgcggatatc cggcttttcc gatctcttcc
    11761 aaaacaatat ccattttgaa aaccggcaat tcgacaggtt tgcctatgat atcggttatc
    11821 tggtatgtgt aacaacatgg agttttgtct atcctgacaa aatttttgtc ttctatcggc
    11881 atataggtgt cggcataacc gaccaaagcc atcacatccg atgcgatagt gatgccgtca
    11941 tgtcccggtc caattattaa cggcgacttg tttttggccg catacagggt atccggttct
    12001 agtttgtcga ttatcagcat cgcataggaa ccttccagaa gggaaagagt cttcctgatc
    12061 gcttcctcga cagtcatttg ggaaacaaaa tattcgacca tattgatgat gacctctgta
    12121 tcggtctcac ttaaaaagac gacgccgtcc agatatttcg ccatcagctc gcggtaattc
    12181 tcgatcacgc cgttatggac tatgccgaac cgtttggacg cggaaaaatg cggatgggaa
    12241 ttttgttgat tgggctttcc atgagtggcc catctggtat gtccgatccc cagatggttt
    12301 tcgaactcat aagaaaagtc attttctaaa tggctgatcc ttcccttatc cttatatgtc
    12361 acatatttcg catttaaagt atcgtataga aaaatcccgc aggaatcata gcccctatat
    12421 tcgagtttct ccaggcctga caagatgata tcactcgcct tgtcattgcc gatgtatccg
    12481 acaatgccac acatctggct caccattctc tctgaaatag ataacagcat tatatcatta
    12541 taatgtgtcg aatacaaata tatgttgatg ttttttatct gaataaattg tctgttgccg
    12601 gttcatcata gatcgttatg atagaaaagg aaacacccgg aaacgcttat caaaaacatg
    12661 atcaaaagga cgccaccgac atccggataa agtactattt gcccattttt cagcataaca
    12721 aacggaaata tataagcaaa tacttccgtt aagccactag ccatgctatc ggtaatctga
    12781 tataggtttg cttccataaa ccagaaaaag gcgagaggaa aaacgattga cagccggtgt
    12841 ttgaaaataa tcatcgataa atcggatata acggaaagat aaaccgaaaa aacaaataaa
    12901 gataacatgc tttccttaat gaattccaat gatacattct ccttgaaata gatcttagct
    12961 ccgcatgaaa gcaataatac ggacaaagag acgaaaaccg aaactatcag ccagaccgcg
    13021 attgatttat aaagccagaa aaacgccttg tcacggcgtt ttcttaagat ataccatcca
    13081 tatagcgaat attccttgct tgcagcttcc gaagccatca gaaccgcaga caaagcacac
    13141 agaaacttta tttgcaaaag cgtatctgac agatatgaga cataatattc atctctcatc
    13201 atgtcgatgc cgctagtacc ctgaataata cccgaagcgt atgtaattcc tagaaaaagt
    13261 aaaacctgga atcctatgaa tatcaggatt actttccggg aaagaagcaa attcagatgc
    13321 attttaattt gagccatcgc gcaaaagcac tccgctttca atttcgtaaa ttctttttgt
    13381 gtgcgatcgg aagcattccg gataatgcgt ggaaacaata acgatattct cccggcaaag
    13441 ccgaaacatg attttaacaa tcactttctt cattttgccg tcgagtgagg aaagcggttc
    13501 gtcaaacagg tatattctcg gttttccgat gaatgcctgc aatagattca ctttctgttt
    13561 catgccgttg gacagttttc cgatcggttt atccattaca ttagacaagt cgagcaagtc
    13621 acagtattct tgcagatatt cgggaatccg tttttcgtga tcccgtctga ttcttccgat
    13681 tgacacaaga aaatcattaa tcgtcatgaa ttccggcatt acatattttt ccggagcata
    13741 tccgatgact cctttgcttt tatgaatctt tccctcatat cttacaagac ccataataca
    13801 ttttaaaagc gttgtctttc ccgaaccgtt tcctcctatt aacaaattca ataatccggg
    13861 gctgaaaaac gtgcttatcc ccaatatgcc ggtattatgg cgataaactt tttttaagtc
    13921 atcgatagtt ataatcagtt ctggcgatgc attcatcttc aatatccgaa aaagccggca
    13981 tggattcgta gggcatgtcg tcaatcaata gattatactc ttcgcccatg tattcgtatt
    14041 tgatcgaaat gtagaatcgg cggagcataa aaattccctc ttcatattga atcggaaata
    14101 acaaataacc atcttcttcg agtgtgattt ttgtatctaa ccggtatgac atttcaggat
    14161 cgtatgacgt tatcgaaagc aattctgaca tgtcttgcgt ataatcgaac cgatttgtgc
    14221 tatatgcata ttccccggtt ttaattttcg tttttaaaga accgatcgaa acatcgtcga
    14281 taataacgtc agaaagcgat gcgatttcaa gtccgataca aataccgctt aggtatgttt
    14341 tctcctcata ttcaccataa atgccgtaaa cgctgaaaaa atcaaggcat ccttcagtat
    14401 ccagatcaca aaaaaggaaa gatagatctc cgatagaaag agaaatctcg tcaccgtttt
    14461 tgtatacaat atgaaaacat gcatccggga aagccatgac tccttcaagg ccaacggaat
    14521 caaatccgta tttgacttga aagcggtgat attcttcatt attccataag acagtttctt
    14581 ctagatcctc aattccggtc aaattgaccc ggataacgtc ctgattactt tccatgtagg
    14641 cttccgaaat gcattcgtta tcagtcaaaa aactatcggt tttcgtcaga tacatatcga
    14701 atgattccga atcgtcgtcg tcaatcaacc tcgattcatg gtttatgacg gtgataatcg
    14761 atatcgtatc aacctgttta ccaggaatga tcaggatagc tgccatagtc aatacatata
    14821 gccctataac aaaagcaaac cattttttca tcgaattttt tccttgacga tttcataata
    14881 ttcggttttg acagtgaata tttcccaacc gccttttttt gtctctatcc aacaaaagta
    14941 ttttttcgct accccgttgg aaacctcacc gacgcctctt gtgacgatag taacataggt
    15001 tttcggatca acgatgatcg tgaactcata ggtttccgat gtggtcgtcg tcttggtata
    15061 tgtcaccgat gctttaatat cggcatcgat gcttcccttg aatttcttga tgttcccatc
    15121 gacgcttgtc gatatcgatc cagaggcgct cacttggaat tttgtctctt cactggtttt
    15181 taaggtgatg tcgtgtttga tcgtcgaata tccgtcgtta tagattttca attttgtttc
    15241 cgagacgaac tccgcatttt cgttctcgtt cacgacataa agcttccatc ccgaaaaaca
    15301 tttcttcgta atttttgtgt aataggtggc ataattcgtc gtggagaaat ccttaagaag
    15361 cattgcttcg tcgtcgtcaa acacaatttc ttgataattg agatattctt ccgcttttgc
    15421 gtttgtggat aatagtggca gaaataacga aattaacaac agaaagtaaa atcctttttt
    15481 cataaaaaaa cctccccttc gcaaatatct tacgttggga gaggctttta ttcttccaaa
    15541 aaatctgaca aaatgagtgg atcgatggtt tttttagttt cttcgtatcc aagttcatat
    15601 aactgtcgac agcgttcact gtcgaatttc aaaacgcttc cgagtaatct gctgtgcttg
    15661 atttcaatta ttttaagttc cggatactta tctctttcaa ggtaatcgaa acgattaaga
    15721 ttgacgacga tcgccttatc acatccgttt gcgaccaaag gcgttaacgg aatattgtcg
    15781 tataacccgc cgtcgtaata gttatgtccg tcgatccgaa ccggcggaaa gacaaacgga
    15841 atcgagcatg atgccatgac ggtttttctg atctcttcat cggtctcgtt atccagggga
    15901 acataggcta ctttgctgtc gagtttgatg aaatgttcgt aattcgcccg catgaggcca
    15961 aaaaatccgg tgctcactgc gcctccgtcg gaaatggtcg catatacatg ctttcctctg
    16021 attttttccg gctgcaggac tttcatcaaa gctttttgga aagcgtcaat ctggaaacat
    16081 cctttttcga caatattgga tcgttccttt atcaaaagcg aaatgaaatg ttcggtttgg
    16141 acgacaattt cgctgttcac cgaattccat gcttcgagag cgtatgcaga ccctccggat
    16201 gaaaccagac agctgttgac cgatccgatc gaggttccgg cataacatgt gattttgtcc
    16261 atgataccca tttcttccaa ggccttgacg gcaccgacct gatatggtcc cctcgctccg
    16321 cccccagaaa ggcataatcc gatcttagac atctagatca ccaccgttcg gggattatct
    16381 tccaagaata ccttgtcgat cgttccgccg ccgagacatt cgtcgttacg gtaaatcacg
    16441 cattcctgtc cgggggtgac tgcccggctt ttttccggat acatcaccat aacggaattg
    16501 tccggagctt tcgataaatt gaccgcgaca tccttttgcc gataacgaaa cttggcagac
    16561 aaattattta tgtcatctgg catgacgccg ccaatccagt tgaaatccgt taccaggcat
    16621 cgatctgaca tcagtagatc attgtagaaa ccctgacaga cgtaaatatc attgtttttc
    16681 acatctttgg catagacaaa ccaaggtgaa ccttcataaa tgttgcatcc gccgattccc
    16741 aggcccttgc gttgcccgat agtgaaattg gcgatgccac gatgcctacc taaaatattt
    16801 ttgtccgaag acaggattct cccttctcta ggcttcagat aattaccgat aaaagcgggg
    16861 aagttccttt caccgatgaa acaaatcccg gtcgatcctt ttttttcagc aacggaaaag
    16921 ccctcttttt tggcgatttc cctaacttct ttctttgtca ggtttccgat cggaaacatt
    16981 gtttgaagca attcgtcgcg tgagagttcc gctagaaaat aactctggtc cttattggga
    17041 tcgacaccgc gcaaaagcac gccgttttct gttactctgg cgtaatgtcc catggcgatt
    17101 ttatcggcgc ccagtgcttt tgcgacatcc agaaaagccg aaaatttgat gtatttgttg
    17161 cagaggatat ccgggttagg agttctcccg taatccagtt ccttaagaaa gtattgaaag
    17221 acatcgtccc agtatctgtc gacaaaatcg tgacggtgca gtcttattcc gagtttcttt
    17281 gccaccgtca aggcatccag atagtctttt tcctgcggac aaatgccttc tttggtcgta
    17341 ggggcaccaa ttgtttcact gttgataaga ctgtcccaat tgcgcatgaa cagtccttcg
    17401 acttcatatc cctgtctttt caaaagtaaa gcggcaacgc tggaatcgac gccaccgctc
    17461 aatccgatga tgattttcat attttgttac ctgtccatat cggaaggcaa ccggaagtcg
    17521 gtgcggggag acagtgccac atccagcact ttcggggaat ccgggctcgc ctgacgcttg
    17581 aactgctgag agaaaaatcg tttgaagaaa ttcgatacat atgtctcgca ttccgcttga
    17641 ttcatgtcga aagtcttttc gagaagaaat acgatgcggt cggctccgtc tccgcaggaa
    17701 aggaatctat aaaggatgaa gtcgttgatt tcatatttcc cgatgacgct ttctgtcgtc
    17761 tgatcgcttt tcagctccgg agtgatcgga gtcgcgagga tgtcgtagag gcacaaactc
    17821 acatcttctg agaaaaccag atcggcatat tctttgatca tgaaagacac gacggttttc
    17881 ggaatgccgg cgttgacatt gtacatgctc atttgatcgc cattataagt agaccaacca
    17941 agtgcgatct cgcttaagtc tccggttccc aaaacaatac cgtctatttc gttggctagg
    18001 tccatgagaa ccatcgtcct cgctctggcc tgggcattct cataagtcgt attttccgta
    18061 gtcgaatcgt tcctgatcaa ctccagatgt tttttgacgc tctcggaaag atcgatttcc
    18121 ttaacggata ccccgagact ttccatcagt ttcttggcat ttttatttgt tctgccgctc
    18181 gtatgcaaag ccggcaatgt caatgccaat attcccttgc gatcaagttt gagcgtatcg
    18241 aaagcccggc aggcgacaag caaagccaaa gccgaatcga gtccgccgga tactcccaca
    18301 agcaaatgtt ttatcttgag atgatccaga cgtttggcaa gcgcatattc ctgaatcgac
    18361 gcaattttac gaaaatcgcc ttctatgtcg tctttgggaa caaatgggaa tttctccaat
    18421 ggacgtgaga aaataaaatc gtccgaagtc gccaaagtaa ccggaacatt ctgaaattga
    18481 taacggtatt tgagaatcga atcacggaaa gatgagttgt tgcgacggtc attggttatg
    18541 cggtcgatat ccagatcggc gaacagttta atggtattca aggaaaattc ttcagtctct
    18601 gccacgatat gtccgttcat ggccacgatg tcgtggccgg aaaaaaccat ttcgctggaa
    18661 gattccgaag ctccggccga acaataaagg taaattccgc agttttttcg cgaatgttcc
    18721 ttgacggcac ttcttctaat ctcttcttta ccgagggttt catttgaggc actgatgtta
    18781 atgattaaat tggctccgtt gacgctcatc agatttcccg gagtaattgt cgcccacatg
    18841 tcttcgcaga tctcgatgcc gaacctgaca tttccaaacg aaaaaatcag atttccgaac
    18901 ggaacgactt gtcccatgat atctgttttg gtgatatgtt cgacaacatc aaatccggat
    18961 ttaaaccaac gtttttcata gtactccctc gtcgagggaa gataatattt cggtacgatt
    19021 ccgagaatac ggtctttttg gatcactatg gctacattga gcaccatttc ttcaacgacc
    19081 agcggtaatc cacatataac aagtccggga taccgatttt ccttcaaaaa ccgtcctagg
    19141 gctttttcga cgtcgtcaaa cagggcttta tggaagaaca tgtccccaca tgtgtaaccg
    19201 gaaataccga gttccggaaa aacggttaaa gaactcggat tgtctttcag gatagacagc
    19261 atttcggata cgttgtagtc gggattgccg accgcaagtt tcggagtcgc cagacagatt
    19321 tttacaaatc cctttccaat catttgacta acctctgtag agaccttggg tctcgatata
    19381 atcatatact tcgtccgtca gcatttcccg gtcgaacgaa tcacggaaac cggtcgatga
    19441 catttccata tggaaatcgc ggatgatgat gaaacgatct agataatcac gtaaaaacgg
    19501 gtcatcggaa ataagtttgt cgatatctct tttgtcgcgg ttcatgacga tgaaccggaa
    19561 ttcggccaat aggcttttcg cattgatcca tttatggagc tttaagagat tgtcggcgcc
    19621 gatcagaaat acgatttccc ggtcgggata tctttcctgg aatctgagca tgctctgata
    19681 tgtgccgaga tagtcggcat cttccagttc catcggggaa acttccgcaa ccttgtccct
    19741 gccaaccaaa agggaaagca tctcgaaacg atgctgattg ctggcgagcg aacgtttggt
    19801 ataaagagag cttaccggca aaaagatgaa tttttcgcag tcgattgttt ccctgactcg
    19861 cctgtaaatg gcgatgtggg ctttggtcgg cggattgaat gcgcctccga agattacgat
    19921 caaatcaagt caactcccat caatggatga tttcaattaa ttttaacata taaccgcggg
    19981 aaatgaaaga ttttatcatg tttccccgcc tttgttatgc tataattgtt ttgaggtgag
    20041 ctaaatgaaa gaaacattgg attacatatt tgatctggca aaaaagatcc tttccattcc
    20101 ctccccttcc gggtattgcc gcgaagtgat tgattttgtt tcaaacgaag cggaaaaaat
    20161 gggatttaaa gttgaaagaa cagtcaaggg caacgccctg atcaaagtca aaggaacggg
    20221 aaatttaacc ctgggactat ctgcacatgt cgacactctt ggcgcaatgg ttaaaagtat
    20281 cacatccgac ggatacctcc gtttcacgac gatcggcggg ccgatctggc cgacgctgga
    20341 cggtgaatac gtgatgatca gaaccagaac cgggaaaaaa tacgacggaa cattcctgtc
    20401 gacttctcct tctgtccatg tgtacaaaga cgccatgtcg aaagaaagaa cgccggattc
    20461 aatgatggtc agaattgacg aaaccgtcca ttcgaaagac gacgttataa aactggggat
    20521 agctcccggt gattttatct tcatcgaccc caagacgacc atcaccgatt cgggatacat
    20581 caaaagccgt tttctcgatg ataagatatc cgttgccatc ctttacgggc ttcttaagga
    20641 aataagcgaa aaccgcgtta aacccaaaat gaatctggaa ataattattt ccacttacga
    20701 ggaagtcgga cacggcgccg cgtcgctgcc acatgtagac gagctcatag ctgtcgatat
    20761 gggttgcgtg ggcgacgatc tttcctgcac cgaggaaatg gtatcgatct gctgcaagga
    20821 ttccaccggg ccgtacgatt atgatgtcgt gacaagactt gtcgaaagcg caaaagtcat
    20881 aaaagccgat tacgctaccg atatctatcc tttctacgga tcggatgttt caagcgccct
    20941 gcgcgccggt cacgatgtca aaggcggact gatcggtccg ggtgtccatg cgtcgcacgg
    21001 aatggaaaga acgcacagaa aagggataga aaacaccctg aaactgctga tttcctacat
    21061 ttcatgattt ttgtccctta tcctattgtc aaaaaaaaca atatacttta taatctaaaa
    21121 atgtagtgaa aaatataaaa taaaacggag gagaacaagc aaatgaaaca atttttcaat
    21181 gtattgggtc aacttttcgg atttttgacc attctgttat tcgcttttat ctttgcaaac
    21241 gaacttttcg gtttcggagt cagcgcagac atcatcaata ttcttgaaac agtcaaggtc
    21301 tatgccgttt tcggagtttg cggcctggcc ggattcgaaa tggtggcggg aaagaaattc
    21361 ttcgcgttcc tgtatttcat cattctggcc ttcgtcgtca tcatgagttt ttttccatca
    21421 gtcggaaatc tcatcagaag ttacgtaaca acattcatag cttgaaaaaa gcgcaggtcg
    21481 ccgacctgcg ttttttttat attagtgctc cgtgcatttt catgaaagcc cggatttctt
    21541 ccgatgccgg caatcttact tccacgtcct gatccttgat gggatgccgg aacctgataa
    21601 gattgctata gagcaacata tccttgaccg gataacggtt cttgttatat agaagatctc
    21661 cgacgatcgg ataattgaaa taagaaaaat gcacccggat ctgatgcgtc cttccggttt
    21721 cggaaaggat cctgacaagg gagaaaccat cgaattcacg ttctacttgg taggatgtct
    21781 tgcattcgtc tcccgtttcc gaaacttccc gcttgataga gccttcactc cggctgatcg
    21841 gaagttcgat gcagaaacgc ttgatatcca tcaacccttg gacgactgcc aagtactcat
    21901 tggtgatgga gttatcggta cggtcgctaa acaagaattt cagaaaacgg tttttggcca
    21961 ccagcatcag cccggacgtt tccttgtcca gacggttgat gaaatggatt tgcgagccca
    22021 aaccgttttt tttgtaataa aatgcgaggg cgttcgccaa tgttcccgtc ggatgggttt
    22081 tggaaatcat cacctgcatg tcggaaggct tgttgacaac gagaaaatat tcgtcttcga
    22141 aaaggatgtc taaaggaatg tcttcgggga taatatccga ttcaggcttt tcgtcctgga
    22201 ttcgaatctt tattgtgtct ccctttttca tcgtgtcgtc cttggttttc agatcgttgt
    22261 tgacgaaaag catcattttt ccctcttccg actttacgag tttgagggga atattgtttt
    22321 caagtaaaaa tcttttgata gtttccggct tttttgcccg gtagataaga tccataggca
    22381 gtcacttcct cgcttatagt ataacatata aagaccggaa ggacaaaatt atccgaccgt
    22441 ttggaacgtc atttcagatt ttctggattc aatcccaaga gctctggtat tacaaacatt
    22501 ccgtccttgc ggatcaggat gtcgtcaaaa tatatttctc caccgccgta ttccggccgc
    22561 tggatctgga ccagatccca atggatggct gaatcgtttc cgtttggcgc ttctgtatag
    22621 catcttcccg gtgtgaaatg gatgcttccg gcgatctttt cgtcaaaaag aatgtccccc
    22681 atcggtcttg ttaccagcgg attcacgccg atcgcgaatt cgccgacata tcttgcgcct
    22741 tcatcggtat tgaagatttg ttccaggaga tcattttcct gatcggcaac cgctttgatt
    22801 atctttccgt tttcaaaagt caagctgaca ccggtgaaga caactcctct ttgcggcgaa
    22861 ggcgtattgt aagttattgt cccattgact gaatttctga ctggagccgt aaaaatctcg
    22921 ccgtcgggaa tgttgttgtt tcccgcgcat ggaatggcat tcattccttt aatcgagaaa
    22981 gaaagatcgg ttcccttggc gaccaaacga accctgtcgg tcttttccat cagatttttc
    23041 aggggttcga aagccttgtt catgacagaa tagtcaattg ccgaaactcc gatcacataa
    23101 tcgaaataag cgtcataact cattcttgct ttgtgcgcgg caccttcggt cgggtaattc
    23161 agaagtaccc atttttttga aaggtttttc tccgagagcg gattgagttt cttcgcaatt
    23221 ttcagtttgg tttccatcgg gacgtccacg tccgtgtagt cggattccga cgctctgata
    23281 gatatgatac aatcaagatc atcgagtttg gcatccatcc atctgtagga acggtccagc
    23341 catttgtcgg acattccgag cataacctcg cgtccgatgt ccggatcgga aagttcgacg
    23401 atcgcatttc cttttccttc cctgatcgct ctcaccaagg ccaggatcaa aggcttagct
    23461 ttgacaggtg ccttgatcag gacgttttcc ccttccttca tactgatgga atagttcaca
    23521 agcatttgag ccagtttttc gattcttttg tctttcagca tttgtttttc gctcctatct
    23581 ttttgaatgc ggcgtattcc gatttcacta ttgaatatga gaatggaaat tcgcgatttt
    23641 ttattctttt ttcgagatcc tcaaattcgg tttccaaaat ctttttcaat tcctccgaat
    23701 atgacatttc ctccgaatga cgtcgatatt cgtttttatc taggatcgta taactaagat
    23761 ccggcatcac cttgatgtcc aaatcgtagt cgatgtattt gattccttct tcgtccgtta
    23821 acaaaggaga agacaaattg caatagaagg aaattccgga tgatctgatg atgccgatca
    23881 cattgtacca gtgaagcggt gaaaaaaacg atacgcatgg ttcctttgtg aaccaaaagc
    23941 ggccgtttcc ttcgatgact ttggtttttc ggttggccac aaccatccat tcgtcctcgt
    24001 ttttaacgac aacggattct tcccagattc tatggagagt tccgtcgtgt ttgaaactga
    24061 ggatctttat ttttgtacca gggatatatt ccataacaaa tcagactccg ttatcatatt
    24121 ataacagaaa aaaagaaaaa tggaccagcg aatccgcact tggtccatga aaatatgaca
    24181 ttattccttt tccagccaac aggcgccctt gagactttca ggctcttccc ggaaaagacc
    24241 aggatatcca agttgccgta aaacctcgta aactgctata gatactgtat tagcaagatt
    24301 gagacttctc accttatcgg tcatcggcag acgcaaacag tcatccagat gctcgcgaag
    24361 gattgctttc ggtattccgg tggattcttt cccgaaaatc agatagatgg attccccggc
    24421 tttagtgaag tcgcattgat cgggactttt tctgccatac ctagttagaa agaaataccg
    24481 tccgggattc ttaatcttga aatcctgcca atctttgtaa atgtgatatt cgagatgctc
    24541 gaggtagttt accccgctcc gtttcaaaat agacagatcg aatttgaatc caaacggttc
    24601 gatcagatgc aaaactgctc ccgttcccac cgaagtacgc atgatattcc cggtattctg
    24661 ggggatttcc ggattataaa gcactatgtt gattttcatc tcttttcccc cttgttttct
    24721 tgtatgtcgg attctttccg ttctaagtca taaatttaaa tcgaaaatta ttttttaatc
    24781 tttgaagatt gttttatccc gctattttgc ttaaactttg ctttatagtt gggtttgact
    24841 ttaagatttt ttggcctgac cgtctttttt tggtccgttg cctttctttc gtttctttcg
    24901 ttttgtcttc ttgcgggtaa aagttcccga tcgcttacat aacgaaaatc gcatttcaaa
    24961 cctttggctt cgagtttttc aattagcggc atttcttcct tggtatatag agaaacacag
    25021 acaccatcgc ttcccattct tccggttctg ccgcttctat ggatgtaaaa accggtttct
    25081 ttcggtaatt cataatttat gacatggctc acagattcga tgtcgatgcc acggctcaga
    25141 atatcagtag aaacaacata ttggaattcg agtcttctga tacggtcgat gatctgacgt
    25201 cttttcctaa attcgacatc cccgctgaaa agaactgtcg atttcccgct gtctttcagt
    25261 tgttggttta ccgattcggc ggttttcttc gtattgcaga aaacgatggc aagatatgga
    25321 ttgatggcaa gggtaacttc atctaacttc ttcattcttt catccgtttc cgttttcacg
    25381 aaaacatgct gaatcagact ggcattttcc ttatttattt catcttttag aaattccgtg
    25441 tttccaatat atttttttaa aaatggcttg atgttttctg gaatcgtagc ggaaaaaatc
    25501 atgaattgag tatctttccc gactgaggaa gcaattttat ccacatcttc caagaaccct
    25561 ccgtcaagag ccatatcggc ttcgtcaatt ataaaaaaca acgttttgtt tatgacaagc
    25621 gcgttttcgt tcctgattaa atccgcaagc cttcctggtg ttccgacaac aatctgcgga
    25681 tgattctttt ttatactggc tatatcgcgt tcacggtcgc ttccacccga aaaaagcatc
    25741 acattgactt ttggctttga aaattccgcc agtttttcag cgaatttata tgtctgtttg
    25801 gcgagatccg ccgtcggtac gacaatcacc gcctggacag aatccgcatc ttcgcatatc
    25861 ctttcgaata ttgacaacag ataagcatgt gtttttccag ttccggtatt tgattccacc
    25921 accagacttt ttttggaagc ggctttttcc aataccgatt tctgaattgt agtcagttcg
    25981 tcgaatccga gacttgcgag cgcatcattt aagtatggtt tggaaaattt catgaacatg
    26041 cgcctccttc ttcaaaaatc attaatgtta ttatataata atactgaatg aaagtaaaat
    26101 gcaaaggcgg tgacttcatg gaagtattga cgataaatcc ccggggtttc tgccacggcg
    26161 tcatcagagc tgtggaaatt gtagaacggg ctgtcttgga tagatcggtt ccaaaaccga
    26221 tccatgttct cggaatgatt gtccataatc gccacgtcgt cgatgatctg tcgcttttat
    26281 ccgttattac gctctatccg aaatccggaa actattctga acttcttgat tccataaata
    26341 cgggaaccgt agtgataacc gctcacggaa tcgacgaacg gattataaaa aaagccaagg
    26401 ataaaggttt atatgttatc gacgccactt gtcctttcgt tcacaaatcc aaacatcacc
    26461 ttgccaaatg gctatcaaaa ggatatgttg tcgcttatat tggaaagcaa ggacacccgg
    26521 aagcattgag cgcattgtcg ctttcagacg atattttcct gatcgagaat ggacaggatg
    26581 tggataagct tccagaaaat acacagaaat tgtatgtgac aacccagaca acctgtagcc
    26641 aaaatgacgt ttcctcaatc atggaaacaa tcgcaaaaaa atatcccaac gccattttcg
    26701 aagatgagat ttgcaacgct accgcaagac gccaagatgc cctgatcgcg gcaaacaagg
    26761 aaattgacct ttgcgttgtt gtgggcgatc cgtccagcaa caacacaaga aacctcttta
    26821 aaataagtaa agagatcaca aacactagga ccatcatggt aaatgatgtt tccgagatcg
    26881 atccctcgtt tctcaaagac gtcaaacgga tcagcgtcac tagcggagct tccactcccg
    26941 atcgggtcac agactccatc atcgcctttt tacgatctta tgatcagcac gattgatttg
    27001 tttcataagt taaaaaaatc ttttcttttc atgccttttc gtatataatt gtttctgata
    27061 aagtcaatcg aggtgagaca agtggcaaac aacaaaaaga aaccgaatat tacttcatcc
    27121 gaagaaaagg aaatcaggac atataatccg acgaaaagcc atttcggcaa gatcattatc
    27181 gtcatcctgg ctctcggaat gttccttgcg atgttgatag cggccattct cggcatgatc
    27241 gaggtattat agcaactacc atccataaaa acacataaga aaagggttgg aatcaaccct
    27301 ttttcttttt aattttccaa caagagctcc cgagcgtttt ccaaggcgtt ttctgtcacc
    27361 ttgtcaccgg agatcatcat ggcgatttcc ttaacccggt tatcgaaatc caaatatccg
    27421 gcggaagcgg tggttcggtt gcccgttatc tttttctcga ctttcaaatg attggcggct
    27481 acggccacca cctgcggtat gtgggtgatc gacaaaacct gacagttttc cgatatttcc
    27541 cggatttttc tggcaatctg gctcgctaca acaccactga taccggtatc tatctcgtca
    27601 aaaaccattg tttccaggtg ttcggtgcga atgaaaatag tcttgaatgc cagcatcacc
    27661 cggctcattt cgccaccgga ggcggttttc gaaagttctc tcagaggttc gccgggattg
    27721 gtggaaatga agaaatcgat agaatcaatt ccgtcctgac cgaaattatc cggattgtcc
    27781 ggaatatccg caaatcttat ctcgaatctc gtgttagcca gactcagatc gccgaaaaga
    27841 attccaagtt ccgccgtaat ctgtttggcg gcgtcttttc tcagtttgga aagatcaccg
    27901 gccgattgga taagcgactt tttctcttct tctttttttc tctcgagatc acgaatttct
    27961 tccatacgct cgaaactacc gccggatagc ttttccagtc tatcgcgata ttcgcaaagt
    28021 tccggaatcg tcattttgta tttttcctcg agtttatcca acaagtagag tctcgtctct
    28081 atcttatcga gactgttcgg atcgaaattc aactgatcta gtttcaatat gcattcttcc
    28141 gctatgtcct tgatctcgta ataatgatca gccattcttt cacgaaggtt tttgtattcc
    28201 tccgaataat ccgacaacct gtccagattt tccaaaatac cggcaagtcc ttcaagcacc
    28261 ggttcatcat cgaatctttg ttttatctcc gcaaggcttt cgtacacctt gtcgaagttt
    28321 ttcatcaaag tacggctttc ctgaagttct cttttctctt ccggatcaag cccagcctcg
    28381 gtaagttcgt taagttggaa aatgtacaaa tcgcgtttct cttcgtattt tcttgtttct
    28441 tccaatagca gggacaaatc gcggacagtt ttctgaaagc cggcaagatc ctgtctgtat
    28501 ttctccaaat aagttccgat cgcttccctg cgatagccat ctaccaaggc aagatagttc
    28561 tggggattta tcagtctttg agtatcgaat tgcgaatgga tatcggccag tctgatggaa
    28621 actgctttta attgttgcaa ggtgacggaa acattgttta tgcgcgacat gttccggttg
    28681 ctgttggtga tttccctcga tatttcgatg gtttcatctt ccatggaaat ccccatggcg
    28741 attatttgtc tttttgtgtc ggcgtttttt atcctgaaac ggccggtgac tttggcgctt
    28801 tcgcagccgg agcgaatcat gtcctggctt gccctgtccc caagcaaaag cgaaatggca
    28861 tcgattagaa tgctttttcc ggctccggtt tctccggtga ggacagtcat tcccggtttg
    28921 aatccgattt ctacccggtc gatgatggca aaattttcca caagcagata ttcaagcatt
    28981 caagattcct ccccacttca catttcgggt ttttcggatg atttgagcaa ttggacgatc
    29041 gatgcatcat ccagggaata atcgttttcc agttcctcgc gagtgccgat tccgatgaac
    29101 tcatctggca aggcgacgat gcttatcttt gaagcgtcaa gtttcaattc gactgctttt
    29161 tcaagaatta aggaacccag tccgccgatt ttggcgtttt cctcgatcac aatgatgttt
    29221 ttcttgtttt ccatgacgga aacgaaagtc gtctcgtcga tcggtttgat gaatctcgcg
    29281 ttaacgaccg cataatcgaa acctttccga ttgatgatat tctcgatcct ggtgacggta
    29341 tccccatagg taatgatggc attttccgcc tcaatattgg aaactgtcca cgatggctcc
    29401 aagatcggtt cgcatatctc gtcgtttctt ttatcggtat cagctttggg atagcggata
    29461 gctaccgggt gtttggcgat ttcgaaggcg taatcgatca ggcttctcgc ttcgcttgag
    29521 tctttcggtt ggattatctc gatattcggt atatgcgaca gataagcgat atcgtaaact
    29581 ccctgatggg tttcgccgtc cgcttctacc agtccagccc ggtcgattcc gaagattaca
    29641 tgcaggtttt gtctggcgac atcgtgaata acttggtcat aagctcgctg caaaaatgac
    29701 gagtaaatcg gtacgaatac atttcttccg ccgattgata gggctcctgc cagacatacc
    29761 gacaaggatt cgcagatccc tacatcggtg atcatggaag gaaaggcttc ctggaaacgc
    29821 ttgagcccgg aaccgttgac catggcggga acaataattt cgaatttatc cccattcttt
    29881 gctttcatga aaagatgatc cgctacgata ttactccagg aagtccggtt ttccgctttt
    29941 ttcgttcggg gaagtccggt ttccacataa aacgggtcga ctccgtgcca ggatccggct
    30001 atgtcgtttt cggcaaatgg gtatcctttt ccctttttcg ttacgacatg aagcacgcag
    30061 ggctttttgg cgtttttgac aagattcagg tattttgtca atgttttaaa atcgtgtccg
    30121 tcgatcggtc cgtaatatga aagcccgaat tcgtcgaaaa tcgtcatgtt tttggcaaag
    30181 cctctcaaca tcctggcgac acggtttttc agatcgatca taaaagcggg aacatttttg
    30241 atcatcctcg ccttttgata ggctttgttg gtacgcatcg agttaagcaa tctggcaagc
    30301 gaaccgacat ttttcgatat gctcatttcg ttatcattca aaatgatgat cggatgcagc
    30361 ccgggacgat gtccgatgaa attcagagcc tcgaaagaga gtccgctgtt taacgatccg
    30421 tcccccacaa gggcaaggca tttgtaatcc ttattatcgc gcatcctggc aatttcaaaa
    30481 cctgccagag cagcgattgc cgtcgatgaa tgcccggctt cccagcaatc atggacagat
    30541 tcggaatgtt tgagatatcc agaaagaccg ccatgctttc gcaatgtggc aaaatcggaa
    30601 gcgcgtccgg tcagtatttt gtgggtatat ccctgatgac cgatatcgaa gatgatcttg
    30661 tccctaggcg agtcgaatac cttgtggatg gcaatcgtta gttcgaccac tcccagattc
    30721 gatgaaagat gtccgcctgt atgagaaatg ctgtcaatca gaaaagaacg gatcgaaaat
    30781 gccaattcgt tcaattcctt tgtattcatc tctttcaaaa atccgggatc ttttatctcg
    30841 taaaggtcag tcattttatc accagcatta ttctttcggt tcttcgaaat cctccaactt
    30901 gtcgtctttc atcagtttga cgatcacgtt ttcggcattt tggatctctt cgtgacaaaa
    30961 cttggcaagg cgcatgcctt cctggtattt ctccaaagat ttgtcaaggg gaagatctcc
    31021 tttttccaat tcgcgaacga tctcttccag ttttgacaag gccgtttcaa aagtcatttt
    31081 ggtttccatg gaatcaatcc tttccgattc tgtcgactgt gcatccgacg attccgtcgg
    31141 acatttgcaa ttctatgttg tcaccttttt caagcaagga aacggttttg actatggtac
    31201 cgttttttct cgccattgca taaccttttt tcatcaaaga taatggattc acaagctcca
    31261 gcctgtcgat gtcgttttca accctggttt ccaatgcaag ccgcagattt tccaaaacca
    31321 gcgaaatcct agttttcaaa ttattcgttt tttccctgta tgaatctaaa atcctgtctg
    31381 gtcttttttg taaaagccgg tcataggcag atgccagttt caattccggt ttttccaaca
    31441 gtctctctcc gtttaatatc accggagatc ttctcaatgt attcagcata tctgttttaa
    31501 cctggatata tttttgccag gagaccgcca tatttcttgt ataaccggat atttgcataa
    31561 gcagggtgtt ttgatcgggg acaactatct ccgccgctcc gctcggggtt ggagctcttc
    31621 tgtcagaaac aaaatcggcg atggtgaaat cggtctcgtg accaacggca gatatcaccg
    31681 gaatctttga ttcgtagatt gctttggcaa ctatctcttc gttaaaagcc cacagatcct
    31741 cgatcgatcc tccgcccctt ccgacgatga gcacatcaga tagaaggtcc ttgtttgctt
    31801 tttctatcat cctgacgatc gaatatttgg cttcgtctcc ctggacaagc gtgggataga
    31861 cgatgattct tgccaaagga taacggcgat tgacgatatg gataatatcg cggactgccg
    31921 ctccggtagg cgaggttatc acaccgatgt ttttgggata ttgcggtaac ggccttttat
    31981 ggctttcgtc gaacattccc gcttccgaca atttcttctt gaggttttcg taagcttggt
    32041 agagatctcc tatgccggat tcatacattt ttcggacata aacctgataa ttgccgatcg
    32101 gttcgtacac gcttacgtat ccttcgacaa cgacctttga tccgtctttc ggttcaaaat
    32161 caagcgagac ggcgtttgac tggaacatca cggccggtat ctgggacgaa tcatctttca
    32221 gcgtgaagta aagatgtccg cggctatgtc gcttgaaatt ggatatttcc gctttaagta
    32281 agatattctg gagattcttg tcattgtcga agaaatactt cagatatcta ttgagggcgg
    32341 atacggtgat gtatttcttt tccatggctt acctcttggt tccgaacaga tcggttttga
    32401 tgttatccaa caggcgattg ttgaagctct ttgccagatt gtcgtcgaga ttcgtatatt
    32461 ttttggttag ttcgagtgct tcgttgatcg ctatctcgaa gggaagatcg gcatatttca
    32521 tctcgtatac cgcatatctg ataatggcga gatcgacata attcagcctg tcgatcgtcc
    32581 agttcgtcag attgcgggaa atgatgtcat cgagatcggg cattttttcc tccaccttgg
    32641 caaacgtggc gatcacattt tcgtcgtatt gcgatttgtc aaaaccgcca cccatatgtg
    32701 tggcgtagat caaatttatg atatcttcgc gtttttttct tgcctgttcc ttgttcatca
    32761 gtccggttta cctttcgtat ttaagtcaat ttattttagc atgtaatgat caaaatataa
    32821 agtataatcg cctcatgatc ccagaagaaa ttcatctatt tttctttctg taaacaacag
    32881 cgtcataagg gtcaagttct actaatcctg atagcacggg aaacgtgtct gtgtttttta
    32941 aaaagggcat atagcctttt aaaatacttt ttggaagtcc gattgccgtt ttttcctttg
    33001 tgaaattaca tattacgaga aggctgttgt tttttccctt gttttcataa acgaaacttt
    33061 catcgctgcc tttatcgatg aaacaggctt taccagcata aatatccgaa tcagtttttc
    33121 tctgattcag aatcatccgg tacaaattaa gaacggaatc gggatcggcg tcctggtttt
    33181 cgacatttat ggtttcatgg tttccattaa ctttgatcca aggttcggat tgggaaaagc
    33241 cggcgttttt ccccgccgtc cattgcatgg gcgttctggc attgtcccgg catcttcttt
    33301 ttattattcc cagcgcctct tcctcacagt acccccggtt tagaaaattt cggtattctg
    33361 taaagacctc gacatcacgg aacaaggaga tatcattgaa atcaacattc gtcataccga
    33421 tttcctctcc ctgataacag attgaggttc ccggtaggaa atacaaggcg atagctaaca
    33481 ttttagcgga ttttacgcgc catttcggtt catcagatcc gtaatggctt acgatacgtg
    33541 gttgatcgtg gttatgccaa taaatgacat tccagcccat ctcatcaagc atctcgtacc
    33601 atctatcgaa actttccttt attttgtgga cctgaagttt tccttctgac cattttccca
    33661 tcagtctggg attattttca tccgtatccg cccaacaatg accgaactgg atcagaagat
    33721 tgaattcgtc tgatccgaat ccgacatatt gaagcgcctc tttctgagat gcgcctccag
    33781 attcgccgat tacaagcaaa ttgttcggga caaaaagttc cctgccgagt tctctcagat
    33841 attcgtgatg tttcggcaaa cttgaaaaat gattatatcc gggataatca tccttaaatt
    33901 cccaatcttt ttccagatgg tttgaagcat ccactctgaa tccgtccact cccattccga
    33961 tccagaaacg gatgactctt ttcatctcaa gatgcacttc tggattgcgc cagtttaaat
    34021 ccggcatttt ttttgagaat atatgcagat aatattcgtc tgtcgcttcg ttataatccc
    34081 agaccgggcc tccgaaccag gaaatccaac gtgtcggaag cgttctttct cctgtcgggc
    34141 ccttttttgg cttttgccag atatagtaat cgtgatattt ttcatattcc ggattgcttg
    34201 ggtcgcgtgc agccacaaac cagggatttt catccgatgt atgattcaat acgagatcgg
    34261 tgataacctt gatcgataga ccgtgagcct tttctatcaa tatccggaaa tcgtcaagat
    34321 caccataatc atcgctgata cgataaaaat cgcggacatc atagccgttg tcgtccatcg
    34381 gagaatcgta atgcggggaa atccaaatga ctccgattcc cagttttgcc aaatacggga
    34441 gtttctccgc cactcccgcc aaatctccaa tcccgtctcc gtttgtatca taaaaactgc
    34501 gaagatagat ctgatatatg ttttccttca tccaccattt ttcctgcatt gtctgcctcc
    34561 gctttattca atccgccagt tgtcttatat cgatggcggg atagtattcc cccataacat
    34621 attcgatttt gttttctgaa agaaatgatt tttttaatgg cctagcctga tgatcgaaca
    34681 cgattccgtg gctataagcc tgttggcata catggtacca ctccgggacc gttttatcga
    34741 attcttcgga tgaaatgacg ccgaacaccg cttctctcat caagggcaaa tcgtttcctg
    34801 ttgcatcgtt aagccattcg gaaaaagcga acgaataccc gccttccgtg attgattttt
    34861 tgacttcagc cagttttttt cggcttgaaa acgggctttt tatcatgaaa atgattctct
    34921 gaggatcgat tttcgctttg ttagtttctc tgatcaggta agaaatcatt tccggtctca
    34981 tgagaaggta tttttctatg ggatatagaa acacagttcc agaatcattt ttcttaagaa
    35041 aatacttgac atccttcaca aaacgcttgc tgaaagcatt ctggtattcc ctgaccttgt
    35101 tgaaaccgac aagagcgaga tccaactgtt cctttgaacc gaaaagcgaa cggctgttga
    35161 gagccgcttc cagccagaat attttctttt cctgccatct tccgatcgga tggtattcca
    35221 gacttaaagc tccttcggcc agaatccggt tggtcgcttc tagaatgctt acggatagtt
    35281 cgtctgaaaa cgatctctgt ctagaggtat aaaggacatt ctcctcttca tttctcgata
    35341 cggacatttc cagaagctgg cgcctgatta ccttgaaatc agtgggccgg tttatgacaa
    35401 gcgcgtttgt cgatatctgc atgacgggat acagtatttc ctggtaatcg atacttctta
    35461 attccctggc tatcctttcg atcattctct tgtcatttgt ttctaaaagc agaacaaacg
    35521 acgaagaaga tagggcataa agccctaaaa ggcggcttct tgcgctcttt tctgccatat
    35581 ccttgaatct tacagccagt cttgccggaa cggtttcatc gggcaaagaa tagacttcgt
    35641 ccatattctg gaaattcacg acgagataag atttctgttt gaaaggaccg ttgttggctt
    35701 tggcaagtcc ggacataccg ggcatcatat aatattcacc ggaaaacgac gcttctttgt
    35761 tctcggtatc ttgtatcagt ttatccgtca tgtcctttac gagacattcg atcgccaagc
    35821 tgcttgtctt gttaatgaac tgcaaagttc ttacattacc tgacacattg tatttcagcg
    35881 tcattttttc cgtaaccatg aagtcatccg gaaaaagacg tttt
//
LOCUS       DAOPJL010000012        30592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1586_length_30592_cov_7.375970, whole genome shotgun sequence.
ACCESSION   DAOPJL010000012 DAOPJL010000000
VERSION     DAOPJL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 30592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30592
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_30592_cov_7.375970"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLP02_02795"
     CDS             <1..834
                     /locus_tag="PLP02_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42414.1"
                     /translation="VTDMFRIPKYAAYTYMSQKNPNDPVLEVASAMTPGEGDRSILPA
                     TFIFTNCDYVVAYMNGYNLGKFYSEWKVFPNIPHAPIVIDDFIGDKIRENEPYKIKIA
                     DRIKDVLLAYNRHGFSMPLKYKLKMFDLLAFHHIKMDKAMDLYKEYVGNWGSVTPKYR
                     FEGYVNDKLVKTVDIAQPKEAILKVLPDAVTLTHKDTYDVVRVIVKMEDEYGNIRPLA
                     RDVVCVETSEGLALIGPNQMALMNGAIGFFLKTTGKKGKAYTIFRVAGQQPVKIEFTI
                     A"
     gene            complement(879..1748)
                     /locus_tag="PLP02_02800"
     CDS             complement(879..1748)
                     /locus_tag="PLP02_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPF42415.1"
                     /translation="MKKRLIALMLVLIGTVLAGCTFEFPIAITFPSETTTTSYTLPNT
                     ATGTITFQNEDYNSFPTYRSPSYSLSVDDYNSVLLNTRDMIRHSNIQIVSTIYQESSL
                     PWGHTTETVAILEGSGAIFKEDENYYYALTNYHVVDPEEYYGAYEVKAFGDEVFSSAE
                     LVLYNATLDLAVLRFAKNSRVDIHLINITTRTYTKFTPGELVLAVGNPLDIDNNVTFG
                     EFITLQEIANYDYYVIYHSASIHSGSSGGALVDVDGNLLGLNTWGVEDTDESAFAIPN
                     YIIYVFLLNAGMI"
     gene            1928..8899
                     /locus_tag="PLP02_02805"
     CDS             1928..8899
                     /locus_tag="PLP02_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42416.1"
                     /translation="MPILLALTPPALPDNLDFTLYFNILFFSILGLDILFGFIKGFKK
                     SLYGFIVKLIFVIIFFFTIDLVVNFLWSVEIPAMGQLFGMIDSSFSSAASFAQLTDKA
                     VQLYLGDFLGTYATSDQVLALAYSIGMFALKIVYTLLYFTVIQLIYRIFTGIIRLIFI
                     HPSKEEKKYQSKNRGFGALFGLMSGALNVFLVLVILSGIISVSSSISTFATEALPNLP
                     TNIDMEMPRQQIYEANHSVIPLADGENDSMDDAIAMLQEMIDAYNQNIIVSNADKIKI
                     EEISGETPLDIYLFDKVLSIDYNGESISIRHELAVYANVAETVLNSPFFDSNNISDIT
                     GLEIETAFDELAQSNLLTSVIPLALEIAAEKFEVTLPYTLEELNDIPWDEEISDLGTA
                     AALIFDFVNTAGLFDEGTDLETVTFNGDDARDIFEALGDSKLVTMAAFVAVQPLLEAA
                     PENVSMIVTVPTDLDWQTEFYSFGTIVDAVLDTNITYASLTSNNPMEILNSLSSLDFT
                     VLLQSQIISQALVNMFEHAEEIFGEDALSFLDIPSDIIWYDFYDGDTLVHGELYNILY
                     ALNALTSATSDVNFENLSISTIAELDLSSIDAIFESTILVASLTKTLREQEFGDFVLT
                     IPNTAFEDNDYENGYLRKEELINMVDAMAEIVNNLTCDPDDTECEDTGFDLGKAFTLS
                     SSSVEIILESDIVWATLGKQVIDNADEFLLIPDTGGSRETIDVEDETVCVISRLEMQN
                     LFDAISVLGIESIDDIDGLDATLVTDIVSDDAKITTLLTSKIVSATVGDLLYDLGEDT
                     LTIPQEVEMTVYEYETGYTIVNSEEIEKLLKAVGVLEIEDIENMDIGPSFLYNLEAPK
                     ENPEDTPVIDQDKADRLFDSLIIHATISKVILDLTEPEGDEEPVISVPYFSPDNTTLV
                     PDIRYFNDTDSFEWISVDELEDIISAILVLDIEDFNGLSIESLDLDSITDNIGIILES
                     AVLHASISDQIINLNADDIIIPDRNYDDDADIIFTRGIGEQETTYIYDGEIENTLKAI
                     QTLGIQMDDPEVTSSLIDSLALEGSETRELDDQKLDTLFSSSIIQASMSKMILDFAVD
                     DGETSAVLVVPLYSPELLDPADPESDLVIKTVAGTDYISSLELRNLFKAYYVLDIEDM
                     SVVDTLGVDNIFSNLDILLTSAILHATISDQIINMESDAVVIPSINTDDVAVIFTTGS
                     GADETTFITADEIEKTFDALDEMGIDFNNPAFDATIINNLATEIEPGVYSETLDDAKL
                     DIVFDSAIIHASISKMLFDLTEDTPTEEAVLTVPFKDYAGVDIKFTSHEVNYITKTEL
                     KACMNAMYVLDVTTNFDDVESISLTTINENFTTMIASAILHATISHQFLTLETTDIEV
                     PYYNYAGVSIRTTWTTGLDTDEYLDDEELRKTFLALDILGINDLSSPDVSINLTTFYD
                     QPNRDILLASCIAHQAVSKQLVEMGEDTLEIPYFDEDGNAIRITRGVVLDGTNTEYVA
                     TSEIDAMFEVMEQLGILTLDDVDITINISDYYDPSPRGILLASASVQLEISKQILEVE
                     DSILDVPFKNETGDDIRKILGDTLAGTDCEYVSETEIDALFEVLEVFGVTSLTLTSED
                     MTLTMSDLYDETTRGILLSSASVQLKISTEMFNLDNIQEKLTVPSKDVDGISVQFTRA
                     NPDDLVDDLFITQGEINNFMEALEIMAGEDETINDITDFGGSLKLSVLYEAANRTEIF
                     KSATMHATISDQLLHIDLTGEIVVPQTDVNYTVDSVKKDVDGNMYIVEDELDLFFESV
                     ETLGISNDSLDILNFECDINLGTYFKSENPGTYGDNQNILLASAIMQATITKQLLDLN
                     DDYLYVPDTDIDGIQIFENVNGTDFIYVFELKFIIDAVDVLDVGTSIDSINGAFNLGN
                     LSASEDQDTLLLSASMHATISNKLFGIDDNYLRVPEKNQNGTSLVIATGLPMEPTTDF
                     VIKGEIKALINALNIVGLNDLNDVTTEFEPSDIFTNPEIVFLSASMQAMISERIIAET
                     GSSLVIPYLYYGTINEIVIDLTGVVTPTTYIEYTELVALMHSLDLLGLNSISTSWNFD
                     FSQMMTVDDYDTVVASEIIQATISDNLLANVKAETDTPILVTELIVPNYYRQSISAMV
                     NQITESKVQVEQDELGDLLTSLKMLGFTSFTSPVSVTTVTTIFSDPVQSAVFMTSGSI
                     HVTLDHMLRQNSYITVPDMATCDYNGAPTVYNMSGVTTKEEITYFITGMTTMGISDFT
                     DSDISFDTIKNLSPGQQSTVLDSMIIRNKITPDVETACLVASPPYILYNTDYMEDNPT
                     TFLRKDPIILVIAYISE"
     gene            9047..12565
                     /locus_tag="PLP02_02810"
     CDS             9047..12565
                     /locus_tag="PLP02_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42417.1"
                     /translation="MPMTLSYFLQTPNLDFVQYINLIFYVVLGLAILFGFLRGMKKSL
                     FNLIVMTIFYIAFFLTVGPVSKVLWTAEMPWLGQYLEGFVSLPSGFTSFANDFPAILT
                     DLTNNAIDFTNASQEMLDLINGLGQFVIKIVYTVLYFTVILILWKFICWILAAIFVRT
                     RKGESKNRGFGALFGAASGLLSIFIILVTMGGMMSVLGSIATVMTPEEPAEDTTLAYP
                     DRQSLYEASSPIMALEDTASSSQLSDMVVIIQETVDAYNSNIFVKIADKITTSSVSNP
                     EEKVPLHLDLFDRVLSFEYEGETVAFRYELSVLSDVAGVFLNSDFATTNNIADITGDE
                     IATVFDDLGKSNLITTIMPLAIETAADYFDVDLPITRTELYDIDFAIELPAVGGVAAS
                     LFDILNGADMITGEGDVGDITVTPQEIRDLFQDISDSQVIMLAIETVLVPMLEDPESD
                     ISMIVSIPDGIIWEDEVIAIGNIMADIFTADLSFADITGGDTTALLNAAASIEVDDIL
                     DSKLVTYTLINILSGDAGLEAADFLEIPDGIVWIDEDDDNHGELENILIALHSLISAM
                     TDIDFENIGLETIYDLTDTQIDDVFGSYVITATISKIITDMDFGDNPIIIPNNSTVID
                     SQGYIKSEELKTMIKSVRLIVSTTGEDTEFSLESALSLSETDIATLLDSKIIAATVGN
                     LVADMEVPMLEIPDEVTENISALDHSTQVTVISKTELQSLLNAMTLLDLTDFDSISFT
                     SGLLDNFESETVPGTLDQSLMDTFFDSKIILASATNMIQDLDDTDSSILAIPVKDSDG
                     NDLVTTTLGTDYFSTTEMKALFNALFVLSLDSFTSLDMENMTTISDNIDIFLESSILH
                     STISNFILNNTGTVLVVPDDYYGTANPIVISYGSDVYIDSVELSHLVAALSLNSTAFG
                     NISDSWSFDIGSFLAITDLDTLIASEIMQATISANILPYVKTMAESPTVTDFVVPAAL
                     RQSITVDSVNAYQIEANELKHLLNSMKMLGLSTFDSTTDPDAIMTIFNDPALANEFML
                     SGSMHITLDNMIKSNSSVSSSIPDKAYRTSDIYGLSDIILKTEIIAFINAASTLAEAS
                     GSGDFTNITFNFSVMLTLNQTQRQTIVTSMIVRNVLTPDAVTAANLAIPALDPPISAS
                     DYEDGDIGTFLTYDGIVRIMNHLLGE"
     gene            12688..14391
                     /locus_tag="PLP02_02815"
     CDS             12688..14391
                     /locus_tag="PLP02_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPF42418.1"
                     /translation="MIRKISALLIASILSFGLAACVFPFTTISSDSLTTIETEESSTT
                     YTDTQTSAVTTDSTTGAITSTTESTTTATTVTTTTSQPSEPVTIELYSVNDFHGGAYT
                     EIGTISEIGQYVISRRAENPNTILLATGDIFQGSALSNYYHGRPLVDILNVAGCVSFT
                     LGNHEFDWGIEAIGEYSDENPENGEALYTVLAANIVYQSTGDMLPWTRPYEIVETSGV
                     RIGIIGVIGDVIDSIAVSRVTGIEFLDAADTVYEYAEILREEENCDIVVVSIHGYDSY
                     TNYEIAGFSGSHRVDAIFNGHTHWDQANLISRSGVSLPYAQASNGEYSVLAQIVLTYD
                     PNTGSVTNAVANTLALDDLGGTNQEINQILNDYSTRTDYVVFVSEILADSDSDYTRYN
                     LAPWGASVLRDYVSTDVGVLNAGGFRVDMEYGNVTMGDLITIYPFDNYIKTCYLTGSQ
                     LIGLYEDSLSPNIDLVFDDKVTYSGGTLYVDGIAVQASDLYLVGAVDYVFDKSAYVFL
                     DGSQITTTSYLMRDLLATDLRNHPYSGGFNPSDGTAMTLGSICYDFRNFYLSKEETFV
                     I"
     gene            14435..14510
                     /locus_tag="PLP02_02820"
     tRNA            14435..14510
                     /locus_tag="PLP02_02820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14468..14470,aa:Ala,seq:ggc)
     gene            14604..15341
                     /locus_tag="PLP02_02825"
     CDS             14604..15341
                     /locus_tag="PLP02_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF42419.1"
                     /translation="MIELALSIYINKHTKKLIILISVSVLAALMTAFLIYASVFYRSE
                     VNVAEITNVTVEKVNENYYLTPEEDFEIGIIFYPGNKIAAESYLPFLSRFAELGILVV
                     VVDMPYHLTLFGKNKAASIIASYPSVDFYIMGHAEGGEAAARYASKNPDLVKGLILLA
                     SYSKYEVSVPALSIYGTLDTVMDMDEYDANEANLVSSTTIPLIGGNHSMYGDYGLQKG
                     DTETTLTITTLRTEIEVAVRAFIALTA"
     gene            15388..15463
                     /locus_tag="PLP02_02830"
     tRNA            15388..15463
                     /locus_tag="PLP02_02830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15421..15423,aa:Ala,seq:cgc)
     gene            15894..16097
                     /locus_tag="PLP02_02835"
     CDS             15894..16097
                     /locus_tag="PLP02_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPF42420.1"
                     /translation="MNKIAEYIKKQRKAAELTQEQFAIRSGLGLRFIRELEQGKETVR
                     LDKVNVALSMFDMEAVPGKKEKS"
     gene            16094..16426
                     /locus_tag="PLP02_02840"
     CDS             16094..16426
                     /locus_tag="PLP02_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPF42421.1"
                     /translation="MMGAFRKAYAYVRDCFAGVLEETDEGYSFSYDPKYIESENAVPV
                     SLTLPIQNESFISRTLFSFFDGLIPEGWLLSVVTRNWKIPENDRFALLLVACKDPIGN
                     VSIKEVCV"
     gene            16423..17373
                     /locus_tag="PLP02_02845"
     CDS             16423..17373
                     /locus_tag="PLP02_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPF42422.1"
                     /translation="MKCLCCGKTLKTENESGWHKSCIRKFFGTTQIPQIDVDDNTLET
                     LATMSVDKGYTVPGVQKKLSLHLLSQGGTPRLTLVDYPAGYILKPQVEQFEALPESEH
                     LVMCMADAVGIATVPHALIRNGSNYAYITKRIDRVLYKDFVEKLAMEDFCQLDLRLTQ
                     DKYHGSYEQCGKIIKRFSERSKLDLSELFIRIVFFYITGNSDMHLKNFSLIETEVRSM
                     KYVLSAAYDLLPVKVNMPEDREDFALAMNGKKTNIRKKDFFLFAENIGINKEITQKMI
                     AQLVAKKDMLQSMCMESYLPEHLKTSLYELIENRCSVLMS"
     regulatory      17657..17743
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            17795..18685
                     /locus_tag="PLP02_02850"
     CDS             17795..18685
                     /locus_tag="PLP02_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPF42423.1"
                     /translation="MKKTGYELIKKVSWWSIAVNLFLSAFKMIAGILARSSAMVTDAV
                     HSASDVLTTFVVLIGVKLSKKEADAEHPYGHERLESVAGMILAIFLALTGIGIGYSGI
                     MKVISGIEGTLAVPGVLALVAAIVSIVIKEAMFWITRNAAKKTKSTALMADAWHHRSD
                     ALSSVGSFIGIGGAMLGLPIMDPIASLVICLFILKAAFDVARDATNRMVDRSCDEATQ
                     SKMKETISAVNGVKSIDILKTRIFGNRIYLDVEIGCDGTIPLMKSHAIAECVHDRIEE
                     TFPDVKHCNVHVNPYLEKKE"
     gene            complement(18719..19297)
                     /locus_tag="PLP02_02855"
     CDS             complement(18719..19297)
                     /locus_tag="PLP02_02855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF42424.1"
                     /translation="MNELILIRHGETDANRNYVVQGRFDNPLNEYGMKQALETGKFLR
                     QRKEKIDLVFSSPLKRAFMTARLILEGMQTKKSIIVHQNLIERNFGDYDGQKITDEYA
                     DLIKHGTIPNMEKNEDLELRVFETLQEICEKYPGKKIMVVTHSHVIKAILVRLVPGFT
                     YASYLSNCSINYLRYSNGEFFVRKYNISPITV"
     gene            19373..20671
                     /locus_tag="PLP02_02860"
     CDS             19373..20671
                     /locus_tag="PLP02_02860"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HPF42425.1"
                     /translation="MRMVDIIEKKRDGFELTEEEIDFVISGYVKGTIPDYQISPWLMA
                     IYFKNMTDRESSYLTKAMLYSGDVIDLTGIKGIKVDKHSTGGVGDKTTLVLAPLVASM
                     GAKIAKLSGRGLGHTGGTLDKLESIDGCRIDLSETAFIDQVNRIGIAVAGQTATLVPA
                     DKLLYALRDVTGTVPSIPLIASSIMSKKLASGADVICLDVKVGDGAFMKTTEDARTLS
                     RLMVEIGKSFGKKVYAFLTGMEEPLGLAIGNRLEVREAIDTLSGKGPADLEELCVAIA
                     SYMAYSAKAAKTVEEGKILAHENLYNGKALAKFYEFINAQGGHIDDLETFVKPEGIMP
                     YVSRKSGYVQKILAHNIGLASMMLGGGRAKKEDSIDPMVGIVLKKKIGEKVEKGDVLA
                     NIYYDKPVSGEVMEELATAFQISEEPVNSPKVIMEIIGKE"
     regulatory      20706..20813
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            20863..21468
                     /locus_tag="PLP02_02865"
     CDS             20863..21468
                     /locus_tag="PLP02_02865"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HPF42426.1"
                     /translation="MKRNQTLLALLETGIMVAIAVILDAIFGAIYTFPFGGSITPAMF
                     PIFFLAARRGVKYGLLGGLLYGSIQLMFSIPYILSLPQFLMDYIVSFTVLGFSGLIPG
                     QLEKGGRFVLGILLGTVLRLISASIAGLLFWVAYIPEELDFMHTLFGVDIAGFFASDN
                     AAVLFGAFAYNMLYLVPSFIICALIGVIFQKRGLLKFRLEG"
     gene            21547..23493
                     /gene="htpG"
                     /locus_tag="PLP02_02870"
     CDS             21547..23493
                     /gene="htpG"
                     /locus_tag="PLP02_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPF42427.1"
                     /translation="MKETHEFKTESKRLLNLMINSIYTNKEIFLRELISNASDAIDRY
                     HLLSLTDDKLEKTNDYEIHLDINKKLRRLVITDNGVGMTRQELEDNLGTIAKSGTLEF
                     LKEMKEKEEKDKQTVGGIDLIGQFGVGFYSAFMVSKKVQVITKSPFDEKAWKWVSSGE
                     EDYTIEECDKTERGTEITLFIRKNEDGENYDEFLEDYNLRSLVRKYSDYVRYPITMEK
                     ETKKPKLDEKGEEIEGQFDTVIENETLNSMTPIWNKNKSEVKDEELQEFYKSTYFDYS
                     DYLINIWTNVEGALTYKAMVFIPSKAPYNLYSEKYEKGLQLYAKGVFVMDKCKELVPD
                     YLRFIKGLVVSPDFSLNISREILQHNRQLAKISQNLEKKVLSELEKTLKNDREKYEKF
                     WKEFGVNIKYGIYDNFGEKKDMLKDLILFESVKSDKLLTLKEYLEAMPKDQKEIYYAS
                     APNREAVKVSPQMDILKKKDYDVLVLKDDVDEFMIQILREYEKLEFKSINQGDLNILD
                     DKEKEDLKSLESENKDLLESLKDILGNEVKDVRLSTRLSESPVCLVSGEGLSFEMEKV
                     INQMPGEKDVKAEKILEINPKHELFQAIEKVYAANNDLLEDYAWVLYNQALLIEGLPV
                     KDPVSFSDKMVRLMVYAATGKKDE"
     gene            23486..23923
                     /locus_tag="PLP02_02875"
     CDS             23486..23923
                     /locus_tag="PLP02_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42428.1"
                     /translation="MNKLFRQLKKGLKNTFRGFKTKIKTDGDRYGEDLKIAVFAPGGR
                     NNACFRFRHDQASNNLAGFMDIVEWDGRKETSRRSYSFSDVTRMENIPGFSEEEINLL
                     KTAFLYTRENDKIVTVIDPQIQAQIYIEAIGQAFPDIMREIIG"
     gene            complement(23954..24523)
                     /gene="recU"
                     /locus_tag="PLP02_02880"
     CDS             complement(23954..24523)
                     /gene="recU"
                     /locus_tag="PLP02_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HPF42429.1"
                     /translation="MMNYPIKRQSGAHVATSTANRGMDLEKAINDTNQYYIDNDIAYI
                     YKKPIPIQVVKVDYEKRSAARISEAYYKVPSTTDYNGIYKAKYIDFEAKETRSKTSFP
                     LKNIHIHQITHLDNVQRHGGISFIIVHFSLLGRSFLLDSKYVSEFYERSKDGQKSISL
                     DEFEMYGHELKEAYVKRLDYLSVVDKVYF"
     gene            24679..26373
                     /locus_tag="PLP02_02885"
     CDS             24679..26373
                     /locus_tag="PLP02_02885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPF42430.1"
                     /translation="MSQIRFYALGGLGENGKNMYCVEVDGKIVVLDAGLKHPTGNLLG
                     VDAIVPDISYLESRSDDILAVMLSHAHDKNIGALPMLLKRINVPVYGTGFAIAVAKDL
                     LTENRMDPAKYIFNIIGENDRIEFPGFSVEFYATTHSVPESAGVAILTEDGAIVYATD
                     FTFDQNSGSFYQTDFQKLTAFNTHGVLALLSESSGALSLGHSSTDYNLTMLIRETFQR
                     AKHRVIVAMYSTELSNIQKVINEALAAGKKLAIIGRKAQRLVDIGENLGYIQIPADKL
                     VNLRFIDDENQNDFDDVVFLVTGEKHEPFFMLQRMAKGFDRLIHLKKDDTVLMMSYPI
                     VGTEKIAAKTYDTLFRMEVNLIRPNKKILPFFHASAEDIKLLYTLLKPKYIVPINGEF
                     RHQLAQHDLAIEYGYSHEQIALVDNGETITFENGILINQHDMIMNGSLLIDGNFETDI
                     NDAVLKERELLADDGFLMIVANIDSRERVMKNEPEIVSRGFMPLKPADDTYKQIVAIY
                     KDVTTKSFQEANFDWKVYREQFRYEVQKYLYGESGRKPVVIPMVIDVSEDRPCKEI"
     gene            complement(26394..27629)
                     /locus_tag="PLP02_02890"
     CDS             complement(26394..27629)
                     /locus_tag="PLP02_02890"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPF42431.1"
                     /translation="MYEPLASRLRPKTFDEVVGQDKIVRVLKKMLDEDKMVSIILYGP
                     TGVGKTTIALLVADHYPLNHFKFNASSDPKSVLKQIVEAGKLYANTLLVVDEIHRMNR
                     DVQDYLLPYVESGKIVMVGLTTENPYISVNPAIRSRVSIFKLERPSETEIYDYITKID
                     LSSFGKEVVINDDVYRFISIYANGEIRAAINMLEMLMIQAGKKQNEINITFAKDVLGK
                     ASVSADYMGDNYYDILSAFHKSVRGSDVDAALHYLSRLIVAGDLESLLRRLSAIVYED
                     IGLANPGMGPKVAAMCDIARHIGFPEAINGLGAITIEMCLSPKSNSAHLAVNKAISDV
                     EEGKTYKVPSHLINNPTYDKKSPYLYAHDYPGHIVKQQYLPSEMEGTRYYVPQDHTKL
                     EKAFKEQYEKLRKAIDEGK"
     gene            27733..28629
                     /gene="nadA"
                     /locus_tag="PLP02_02895"
     CDS             27733..28629
                     /gene="nadA"
                     /locus_tag="PLP02_02895"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPF42432.1"
                     /translation="MLKDDILVLKKEKNAVILAHFYERPEIQDVADYVGDSLALSRIA
                     ADTTADIIVFCGVNFMAETAKILSPDKTVLLPVKEAGCPLALTINANKLQKYKDEHPD
                     TVIVCYVNSPAEVKALSDVCVTSSNAEKIVSSLKGKKILYVPDKNMAGYLKDKLQIEL
                     DIWPGFCPAHNRISTDDVIKTKKRWPDAKVLIHPEAPLEVLKLADYVGSTKGILDYAK
                     SSDATDFIIGTEEGIIYPLTKENPEKKFHLLPYGLYCRDMKLTHIDDVYNALKTNKHE
                     IQIDESIRKAAKKALDKMLEMS"
     gene            28636..30144
                     /gene="nadB"
                     /locus_tag="PLP02_02900"
     CDS             28636..30144
                     /gene="nadB"
                     /locus_tag="PLP02_02900"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPF42433.1"
                     /translation="MNKKEADVLIIGTGLAGIYTALNINRDHKIMILAKSSLDSSNSM
                     LAQGGIAAELTDDPIRLDSHFEDTLKAGSYLNDREAVEYLVNHAKEAIESLVAFGIHF
                     DKDDNNHYLLTREGGHSRNRILHSGGDMSGYYTTKGLSDLLKDYTNITVKEHTMAIDI
                     IFNEEKEAIGVKVLEKGVGSYPIFAKAIILATGGIGSIYGATTNDLSATGDGIGIAIR
                     AGAEVKNMEFVQFHPTALYQENIENKRRFLISEAVRGEGATLLNIRKERFMNKYSPKL
                     KELAPRDIVSQAIYREMYDTWTDHVYLDTRNLDPEFLKKRFPTIYRTCKEAGYTLGVD
                     LIPVAPCEHFLCGGIKTDLHARTTIKNLYAVGETAMTGVHGANRLASNSLLECVVFGK
                     TAADMINKTIDSIKIESYFESEEMPAYDYNYRPIRKKISDYMDEHVGIVRTSDGLKLA
                     EKTFASIMESLLKSVNMTRYYYETLNMVTTAITITRQALIRKESIGCHFRLD"
     gene            30123..>30592
                     /locus_tag="PLP02_02905"
     CDS             30123..>30592
                     /locus_tag="PLP02_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPF42434.1"
                     /translation="MSLPAGLVKTNYHTHTYLCRHANGNPSDYVKKAIELGFEAIGIS
                     DHAPFSELADRSIRMRPDEYPLYLKELKESIDRYKDSIRIYKGLEIEYLPNKLEYYKQ
                     VLCNDLDYLALGQHYVPDPKSPDGLKSIYDLDNIDQIRSYTYTLTEAMDTGLFK"
BASE COUNT         8586 a         7290 c         6925 g         7791 t
ORIGIN      
        1 gtcaccgata tgttcagaat cccgaaatat gccgcttata cctatatgtc gcagaaaaat
       61 cctaacgatc ccgttctgga agtggcatcg gcaatgactc cgggagaagg cgacagaagc
      121 attttgccgg caaccttcat attcacaaac tgtgattacg tcgtagcgta catgaacgga
      181 tataatctcg gcaaattcta ttcggaatgg aaggtttttc ccaacattcc tcatgctccg
      241 atcgtaattg atgatttcat aggagacaaa atcagggaaa acgaacctta taaaataaaa
      301 atcgccgacc ggatcaagga tgtccttctg gcatacaacc gtcacggttt ttccatgcct
      361 ttgaaataca aactgaaaat gttcgatcta ttggcattcc accatatcaa aatggacaaa
      421 gccatggatc tttacaagga atatgtcgga aactggggct cggtgacgcc aaaataccgg
      481 tttgaagggt atgtaaacga caaactcgtg aaaaccgtcg acatcgctca accgaaagaa
      541 gccattctca aagttttacc cgatgctgtg acattgacgc acaaagatac ctatgatgtc
      601 gtgagagtca tcgtaaaaat ggaagacgaa tacgggaaca tccgtccttt ggcaagagac
      661 gttgtctgtg tggaaacatc ggaaggattg gcgctgatcg gtcctaacca aatggcgctc
      721 atgaacggag ctatcggttt ctttttgaag acgacgggaa agaaaggaaa agcttacact
      781 attttccggg tcgcagggca acaaccggtg aaaattgaat tcactatcgc ataaaaaaag
      841 gcttgacggt tcaatccgcc aagccttgtc tttttatatc agatcattcc tgcattcaga
      901 agaaaaacgt agataatgta attgggaatc gcaaaagcac tctcgtcagt gtcttcgact
      961 ccccaggtgt tcaagcctag caaattgccg tcgacatcga ccaaagcgcc gccgctggaa
     1021 ccggaatgga tcgaagcgga atggtagatg acataataat cataattggc aatttcctga
     1081 agcgtgatga attctccgaa ggtgacgttg ttgtcgatat caaggggatt cccaacggca
     1141 agaaccagtt cgccaggcgt aaatttcgta tatgttctcg tggtgatgtt gataagatgg
     1201 atatccaccc ggctgttttt ggcgaaacgg agaacggcaa gatctaaagt tgcattgtaa
     1261 agaacgagtt ccgcgctgga gaatacctcg tcaccgaatg ctttgacctc ataagctcca
     1321 taatattctt ccggatcgac cacatggtag ttagtcaatg cgtagtaata attttcatct
     1381 tccttgaata tcgctccgga accttccaaa atggcgacgg tttcggtcgt atggccccac
     1441 ggaagactgg attcctgata gatggtcgat acgatttgaa tgttcgagtg acgaatcata
     1501 tcccttgtgt tcagcagcac actgttgtaa tcgtccacag acagagaata gcttggggaa
     1561 cggtatgtgg gaaacgaatt gtagtcctcg ttttggaaag tgattgttcc ggtcgcggtg
     1621 tttggcaaag tatatgatgt cgttgtagtt tcggaaggga aagtgatcgc gatgggaaac
     1681 tcaaatgtgc atcccgccag aacagtaccg atcaaaacca gcattagtgc gatcaaacgt
     1741 tttttcatct gttacctccc gcataattta tcaattttat tataccacca tgtttttcaa
     1801 tattgcctac ggaaacaaaa aatttttttt gtggtcttta aagattcgat gatatataat
     1861 ataataaaaa agtaacacta atataagtga aaaacaaaca atatagtttc cggaaggagt
     1921 accaccaatg cccattttac tggctttaac cccgcctgcc ttgcccgata atctggattt
     1981 cacgctttat ttcaacattc tgtttttcag tatcctcggg cttgacattc tgttcgggtt
     2041 catcaaggga ttcaaaaaat cactttacgg attcatagta aaactgattt ttgtcatcat
     2101 attctttttc acgatcgatt tggtcgtgaa tttcctatgg tccgtcgaaa tcccggccat
     2161 gggtcaactt ttcggaatga tcgacagttc gttttctagc gccgcttcat tcgcccaact
     2221 gacggacaaa gcggttcagc tatacctggg cgattttctg ggaacctatg caacaagcga
     2281 tcaggtactt gcgcttgcct acagtattgg tatgtttgcc cttaaaatcg tgtatacctt
     2341 gctttacttc acagtcatcc aactgattta cagaatcttt accggcatca tcagactgat
     2401 cttcatccat ccttccaaag aagaaaaaaa atatcagtcc aagaaccggg gattcggagc
     2461 tctgttcgga ttgatgtcag gagctttgaa cgtgtttctc gttctcgtga ttctgagcgg
     2521 aatcatcagc gtatcaagca gtatttctac cttcgctact gaagcgcttc cgaatcttcc
     2581 caccaatatc gacatggaga tgccccgcca gcagatatac gaggccaatc attcggtcat
     2641 tcccttggcc gacggtgaaa atgattccat ggacgacgcc attgcgatgt tgcaggaaat
     2701 gatcgacgct tataaccaga acatcattgt ttcgaacgcc gacaaaatca agatagaaga
     2761 gatcagcgga gaaacccctc tggacatata cttatttgac aaagtccttt ccattgacta
     2821 taacggcgaa agcatttcga tccgccatga actagccgta tatgccaacg tggcagagac
     2881 ggttctgaat tcacctttct tcgattcaaa caatatctcg gatattaccg gtcttgaaat
     2941 tgaaaccgcg tttgacgaac ttgcgcagtc gaatctcctg acttcggtga ttccgcttgc
     3001 attggaaatc gcagctgaga aatttgaggt gacattgcca tatactttgg aagaactcaa
     3061 cgacattccg tgggatgagg aaatcagcga tctcggaacg gcagccgctt tgatattcga
     3121 ttttgtcaat actgccggcc ttttcgacga aggcaccgat ttggaaaccg taaccttcaa
     3181 cggcgacgat gcccgggata ttttcgaagc tctcggcgac agtaaactgg tgaccatggc
     3241 cgcttttgtg gccgttcaac cgcttttgga agcagccccg gaaaatgttt caatgatcgt
     3301 tacagttccc accgatctcg attggcaaac cgaattttat tcctttggaa cgatcgtgga
     3361 cgccgttctt gatacaaaca tcacctatgc ctctttgaca tcaaataatc ccatggagat
     3421 actgaattcc ctttcatcct tggatttcac cgtgctattg caatcgcaga tcatcagcca
     3481 ggcactcgtc aatatgttcg aacatgcaga agagattttt ggcgaagacg ctttgtcttt
     3541 cctcgatatt ccttctgata tcatttggta tgatttctat gatggggaca ctctggtaca
     3601 tggagaactg tacaatatcc tgtatgcctt gaacgctctt accagcgcaa caagcgatgt
     3661 gaattttgaa aacttgagca tttcgacaat cgccgaactt gatctttctt cgatcgacgc
     3721 gattttcgaa tccactattc tagtcgccag tctgaccaaa accttgcgcg agcaggaatt
     3781 cggcgatttt gtcctgacta ttcccaatac cgctttcgaa gataacgatt acgaaaacgg
     3841 ctaccttcgc aaagaagaac tgataaacat ggtagatgcc atggcggaaa tcgttaacaa
     3901 tctcacctgc gatccggatg acaccgaatg cgaagatacc ggtttcgacc tgggaaaagc
     3961 ttttacgctt tcatcatcat cggtggaaat cattctcgaa tcggatatcg tctgggctac
     4021 cctcggaaaa caggtcatcg acaatgcgga cgaatttctt ttgattcccg ataccggcgg
     4081 atcaagggaa actatcgatg tcgaagacga aaccgtatgc gtgatttccc gcttggaaat
     4141 gcaaaatctg tttgacgcca tttcggtgct tggcattgag agcatcgacg atatcgacgg
     4201 tctcgacgca actctcgtga ccgacatcgt ttctgacgat gccaagatca ctacgctgtt
     4261 aacatcaaaa atcgtgtccg cgactgtcgg agatctttta tatgatctgg gcgaagacac
     4321 cctgaccatt ccacaggaag tggaaatgac cgtctatgaa tacgaaaccg gatacactat
     4381 tgttaactcg gaagaaatcg aaaaattgct gaaggcggtc ggcgtcttgg aaatcgagga
     4441 tatcgagaac atggatatcg gcccgagttt tctttacaac ctagaagccc cgaaagaaaa
     4501 tccggaagac actcccgtaa tagaccaaga taaagccgac aggttgttcg attcattgat
     4561 catccatgcg acgatttcca aagttattct cgatctgacc gaaccggaag gtgatgaaga
     4621 gcctgtcatc tccgttcctt atttctcgcc agataacacc actttggttc ctgatatccg
     4681 ctatttcaac gatacggaca gtttcgaatg gatttccgta gacgaactgg aagacattat
     4741 cagcgctatt ctggtattag atatcgaaga cttcaatggt ctgtcgatcg aatctctgga
     4801 tctcgactcg attaccgaca atatcggaat cattctggaa tccgctgttc ttcacgcttc
     4861 gatctccgac caaatcatca atctgaacgc cgatgacatc attattcccg atcgcaatta
     4921 cgatgatgat gcggatatca tatttacccg cggtattggc gaacaagaga caacctatat
     4981 atatgacggg gaaatcgaaa acaccctgaa agcgatccaa accctcggaa tccaaatgga
     5041 cgatccggag gtcacttcat cgcttatcga ttcgcttgcc ctagagggtt cagaaacccg
     5101 tgaacttgac gaccaaaaac tcgacactct gttttcgtcg tcgattattc aggcttccat
     5161 gtcgaagatg atccttgatt ttgcggttga cgacggggaa acaagcgccg tattggtcgt
     5221 gcctctttac agcccggaac ttcttgaccc ggcagacccc gaatccgatc ttgtcatcaa
     5281 aaccgttgct ggcacagatt acataagttc gctcgaatta agaaatctct tcaaagcata
     5341 ttacgttctc gatatcgagg acatgagcgt tgtcgacacc ttgggtgtag acaatatttt
     5401 cagcaatctt gacatcttgt tgacatcggc gatcctccat gcgacaatct ccgaccagat
     5461 catcaacatg gaaagcgacg ccgtcgtcat accatcgatc aatacggatg acgtagctgt
     5521 catcttcacg accggatccg gagcggacga aacgacgttc atcaccgctg acgagatcga
     5581 aaaaaccttt gatgccctgg atgaaatggg gatcgatttc aacaaccccg ctttcgatgc
     5641 caccatcatc aacaatctgg cgaccgagat cgaacccggt gtctattccg aaacgttaga
     5701 cgatgccaaa ctggatatcg tgtttgattc ggcgatcatc catgcgtcga tctcgaagat
     5761 gcttttcgat ctgaccgaag atacaccgac cgaagaagcg gtccttaccg tgcctttcaa
     5821 ggattatgcg ggagtcgaca tcaaattcac aagccatgaa gtcaactata tcaccaaaac
     5881 cgaattgaaa gcctgcatga atgcgatgta tgttcttgat gttacaacta atttcgatga
     5941 tgtcgagtct atttcactta caaccatcaa tgagaatttc actaccatga tcgcttcggc
     6001 gattcttcat gcgaccatat cccaccaatt cctgacgctt gaaaccaccg atatcgaggt
     6061 gccttattat aactatgccg gagtctcgat ccgcactacc tggacgacgg gcttggacac
     6121 ggacgaatat cttgacgacg aggaactgcg caagactttc ctggccctgg atattctcgg
     6181 gataaacgat ttgtcgagtc ccgatgtctc gatcaatctg acgactttct acgatcagcc
     6241 aaacagagac atcctgctcg cttcctgcat cgcccatcaa gctgtttcca aacaacttgt
     6301 ggaaatggga gaagacactc tggagattcc gtatttcgat gaggatggaa atgccatcag
     6361 aatcacaaga ggcgtcgtat tggatggcac caacaccgaa tatgtggcga catcggaaat
     6421 cgacgccatg ttcgaagtca tggaacaact cggtatcttg actttggacg atgtcgatat
     6481 cacaatcaat atttcggatt actacgatcc gtcaccccga ggcatcttgc tggcatcggc
     6541 ttcggtacaa ttagagatat ccaaacagat cctggaagtc gaagactcga ttctggacgt
     6601 accgttcaaa aacgaaaccg gcgatgatat cagaaagatc ttgggcgata ccttggcagg
     6661 caccgactgc gaatacgtat cggaaaccga gatcgacgcc ttgttcgaag tgctcgaagt
     6721 cttcggtgtg accagcctga ctctgacatc ggaagacatg actctgacga tgagcgatct
     6781 ctatgacgaa acgacccgcg gaatcctttt gtcctccgcg tccgtacaac tgaaaatatc
     6841 gaccgagatg ttcaatctcg acaatatcca agaaaaactc accgttcctt cgaaagacgt
     6901 ggacggaata tcggtccagt tcacaagggc gaatcctgac gacctcgtcg acgatttgtt
     6961 cataacccag ggagaaatca acaacttcat ggaagccttg gaaatcatgg ccggggaaga
     7021 cgaaaccatc aatgacatca ccgatttcgg cggttctttg aaactttccg tgctttatga
     7081 agccgcaaac cgcaccgaga tctttaaatc ggccacaatg catgcgacga tatccgatca
     7141 gttgttgcat atcgacttaa ccggcgagat agtagtgccg caaaccgatg tcaactatac
     7201 cgtcgacagc gtcaaaaagg atgtcgacgg caatatgtac attgttgaag acgaacttga
     7261 cctattcttc gaatcggtgg aaaccttagg catttccaac gatagtctgg acattcttaa
     7321 tttcgaatgc gacatcaacc tgggaaccta tttcaagagt gaaaatcccg gtacttacgg
     7381 cgataaccaa aacatcctgc tggcttcggc catcatgcag gcgacgataa ccaaacagtt
     7441 gcttgatctt aacgacgatt acctgtatgt tcccgatacc gacatcgacg ggatccaaat
     7501 ctttgaaaat gtcaacggaa ccgatttcat ctatgtgttc gaactcaaat tcatcattga
     7561 tgccgttgat gtgctggatg tcggcacctc gatcgattcg atcaacggtg ctttcaatct
     7621 gggaaatcta agtgcttctg aagatcagga taccttgtta ttatccgcat cgatgcatgc
     7681 gacgatatcc aataaactct tcggtatcga cgataattac ctgagagtac cggaaaagaa
     7741 ccagaacgga acgtctctgg ttatcgctac ggggctaccg atggaaccga caaccgattt
     7801 tgtcatcaag ggcgagatca aggcgttgat caatgctttg aacatcgtgg gcttaaacga
     7861 cctcaatgac gtcaccaccg aattcgaacc ctcggatatc ttcaccaatc cggaaatcgt
     7921 cttcctgtcc gcctccatgc aggcgatgat ctcggaaaga ataatcgccg agaccggttc
     7981 gtcgctcgtc attccttatt tgtattacgg aactataaac gagatcgtga tcgatctgac
     8041 aggagtcgtc acaccgacaa cctatatcga atatacggaa ctggtagcgt tgatgcattc
     8101 gctcgatctt ttaggcctca acagcatttc gacatcttgg aatttcgatt tctcgcaaat
     8161 gatgacagta gacgattacg ataccgttgt cgcttctgaa atcatccagg cgacgatatc
     8221 cgacaacctg cttgccaatg tcaaagccga aaccgatacg ccgatactgg tgaccgaact
     8281 catcgttccg aactattaca gacaatcgat aagcgccatg gtcaatcaga taacggaatc
     8341 gaaagtccag gttgaacagg atgaactcgg agatttgctt acatcgctca agatgctcgg
     8401 attcacaagt tttaccagtc cggtgtccgt cacaactgtc accacgatct ttagtgatcc
     8461 ggtgcaaagt gcagtcttca tgacgagcgg atctatccat gtcaccctcg accacatgtt
     8521 gagacagaat tcttacataa ccgtaccgga tatggcgacc tgcgattaca acggcgctcc
     8581 cacggtttac aacatgtccg gcgtaacgac gaaagaagag atcacttact tcattaccgg
     8641 aatgactacg atgggaattt ccgacttcac agacagcgac atttccttcg acacaatcaa
     8701 gaatctgtct ccgggacaac aaagcactgt cctagattcg atgatcatcc gcaacaagat
     8761 tactccggat gtcgaaacag cgtgcttggt ggcttcgccg ccttacattc tctacaacac
     8821 tgattacatg gaagacaatc cgacgacgtt cctgagaaaa gatccaatca tccttgtcat
     8881 cgcctatatt tcggaataaa tttcatgata ctttaaacag ggggaccaaa cttccccctg
     8941 ttttttatat atacaaaata aagcatttca cattgtattc acatggaaat actgtataat
     9001 tatattgaaa tacgaaatta ttccaccgaa attggttaag gagggaatgc caatgacatt
     9061 gtcgtatttt ttacaaactc cgaatctcga tttcgtccag tacatcaact tgatctttta
     9121 tgttgtgctg ggccttgcca tcctgttcgg ttttctccgg ggaatgaaga aaagcttgtt
     9181 caatctgatt gtcatgacga ttttctatat cgccttcttt ttgaccgtcg gtccggtttc
     9241 caaagtgctt tggacagcgg aaatgccctg gctcggacag tatttggaag gctttgtatc
     9301 gctgccttcc ggattcactt cgtttgccaa tgattttcct gccattctga ccgatctcac
     9361 caacaacgcc attgacttta cgaatgctag ccaggagatg cttgatctca tcaacggcct
     9421 gggtcagttt gtcatcaaga tcgtttatac ggtcctttat ttcacagtta tccttatttt
     9481 gtggaaattc atatgctgga tcttggcggc gatctttgtc cgcacgagaa aaggcgaatc
     9541 taaaaaccgc ggtttcggtg ccttgttcgg cgctgccagc ggacttcttt ccatatttat
     9601 cattttggta accatgggcg gtatgatgag cgtcctcgga agcatcgcga ccgttatgac
     9661 gccagaagaa ccggcggaag acaccactct ggcatatcct gatcgccagt ctctctatga
     9721 ggcttccagt ccgatcatgg ctttggagga tacggcttcg agttcacagt tatctgacat
     9781 ggtcgtcatc atccaagaga ccgttgatgc ctataacagc aacatatttg tcaagatagc
     9841 ggataagatc accacatcgt cggtatccaa tcccgaagaa aaagtgccgc tccatcttga
     9901 tttgttcgac cgggtccttt cattcgagta tgaaggagaa accgttgctt tccgttatga
     9961 actgagtgtg ctgtcggatg tggcaggagt attcctgaat tccgatttcg ccaccaccaa
    10021 caatattgcc gacatcaccg gcgacgaaat cgccaccgtt ttcgacgatc tcggcaaatc
    10081 caacctgatc acgacgatca tgccgcttgc gatcgaaacc gcagccgatt acttcgatgt
    10141 cgatcttccg attacacgca ccgaactgta tgatatcgat ttcgctatag aattgccggc
    10201 ggtcggcggc gtggcagctt cgctattcga tattctgaac ggagccgaca tgataacagg
    10261 cgaaggggat gtcggagaca tcacggtgac tccccaggaa atccgcgatt tgttccaaga
    10321 tatttccgat tcccaggtca tcatgctggc gatcgagact gttcttgtcc cgatgctaga
    10381 ggatccggaa agcgacatct cgatgatcgt ctcgatacct gacggtatca tatgggaaga
    10441 tgaagtcatc gctatcggca acatcatggc agatatcttc acagccgatt tgtcgttcgc
    10501 ggacatcacc ggcggcgata caaccgcttt gcttaatgcc gccgcatcca tcgaggttga
    10561 tgacattctc gattcaaaac tagtcactta caccttgatc aacatcctgt ccggagacgc
    10621 cgggctggag gctgccgatt ttctcgagat tccggacgga atcgtttgga ttgatgagga
    10681 tgacgacaat cacggcgagc tggaaaacat tcttatcgcc ctacattctt tgatatcggc
    10741 aatgaccgac atcgattttg aaaacatcgg tcttgagacg atttatgatc ttaccgatac
    10801 ccagatcgat gatgttttcg gttcatatgt gataacagct accatttcca agatcatcac
    10861 cgacatggat ttcggcgaca atccgattat aattcccaac aactccactg ttattgacag
    10921 tcagggatac atcaagtcag aagagcttaa gaccatgata aaatcggtgc gcctgatagt
    10981 ttccactacc ggagaagata ctgagttcag ccttgaatcg gcgctttcgt tatcagaaac
    11041 cgacattgcg accctgctcg attccaaaat aatcgccgcc accgtaggta acctggtggc
    11101 cgacatggaa gtgccgatgc tggaaatccc ggacgaggtc accgaaaaca ttagcgcgct
    11161 cgatcattcc acccaggtca ccgtcatttc aaaaacggaa cttcagtctt tgcttaatgc
    11221 catgacactt ctcgacttga cggacttcga ttcgatcagt ttcacttctg gtcttttaga
    11281 taattttgaa agcgaaaccg ttccgggaac cctggaccag tcgctcatgg acactttctt
    11341 tgattcgaaa atcattctgg cgagcgccac aaacatgatc caggatctgg atgacaccga
    11401 ttcgtcaatc ctcgccattc cggtgaaaga ctcagacgga aacgaccttg tcacgacaac
    11461 ccttggcacc gattatttct cgacaaccga gatgaaggcg ctcttcaacg ccctgttcgt
    11521 actgagcctt gactccttca cgtcgctaga catggaaaac atgacgacga tttccgacaa
    11581 tatcgatatt ttcctcgaat cctcgattct tcattctacg atttccaatt tcattctcaa
    11641 caacacggga acggttctcg tcgtgccgga tgattattac ggcaccgcca atccgatcgt
    11701 gatctcttac ggttccgatg tctacatcga ttcggtcgaa ctgtcgcatc tcgtcgccgc
    11761 cctctcgctc aactcgacag ccttcggcaa catttccgat tcctggagtt tcgatatcgg
    11821 cagtttcctt gcgatcaccg atctcgacac tttaatagct tccgaaatca tgcaggcgac
    11881 gatttccgcc aatatccttc catatgtcaa aacaatggcc gaaagcccga cagttacgga
    11941 tttcgtagtt cctgcggccc tgaggcagtc gataacggtt gatagcgtca atgcatacca
    12001 gatcgaagcc aatgaactca agcatttgct caattcaatg aaaatgctcg gcctttcaac
    12061 gttcgactca accacggatc cggatgcgat catgacaatc ttcaatgatc cggctctggc
    12121 taatgaattc atgctgagcg gatcgatgca catcacgctc gacaacatga tcaaatccaa
    12181 ttcgagcgtg tcgtcaagca ttcccgacaa ggcatatagg acatcagata tctacggctt
    12241 gtccgacatc attctgaaaa ccgagataat cgccttcatc aacgccgctt ccaccctggc
    12301 ggaagcctcc ggttccggcg acttcacgaa tatcaccttc aacttcagcg tgatgttgac
    12361 tctcaatcag actcagcgtc agaccatcgt cacatcgatg atcgtcagaa atgttctgac
    12421 tcccgatgca gtcactgccg ctaacttggc tattccggcc ctggatccgc caatcagcgc
    12481 ttccgattat gaagacggcg acataggcac attcctcaca tacgacggca tcgtcaggat
    12541 catgaaccat ctgctcggag aataaaaaat caataaagtt ttataggcgg ggaaacccgc
    12601 ctttttcttt tcctggaaca ttactcttta tttttacgtg attttccgtt ataatgatgg
    12661 cacactgcat aaaacgagga cgaggaaatg ataagaaaaa tatcggcgct tttgatcgca
    12721 tccatactct ctttcggctt ggccgcctgt gtctttccct ttaccaccat atcgtcggat
    12781 tccttaacga caattgaaac ggaagaatcg tcgactacgt ataccgacac acagacttca
    12841 gccgtaacca cagattcgac gacgggagcg attacttcca ccacagaatc gactaccact
    12901 gctacgaccg tgacgacaac cacgtcgcaa ccgagcgaac cggtgacgat cgaactgtat
    12961 agcgtcaacg atttccacgg cggtgcatat accgagatcg ggacaatttc cgaaatcggg
    13021 caatatgtga tatccagacg cgcggaaaat ccgaatacaa tcttactggc gaccggagat
    13081 atattccagg gaagcgctct ttccaactac tatcacggac gtcccttggt cgatattctc
    13141 aatgtggccg gatgcgtgtc ttttacccta gggaaccatg aattcgattg gggaatcgaa
    13201 gcgatcggtg aatacagcga cgaaaatccg gaaaacggag aagccttgta tacggtgctc
    13261 gccgccaata tcgtttacca gtcgaccgga gatatgcttc cttggacccg accttacgaa
    13321 atagtcgaga caagcggtgt cagaatcggc atcatcggcg tcatcggcga tgtcatcgat
    13381 tcgattgccg tttccagagt caccggcatc gaatttctgg atgccgccga caccgtatac
    13441 gaatacgcgg aaatcctgag agaagaagaa aactgcgata tcgtggtcgt ttccattcat
    13501 ggatacgact cgtacacaaa ctatgaaatc gccggttttt ccggcagcca ccgggttgat
    13561 gccattttca acggccacac ccattgggat caagccaacc tgatcagccg ctcaggtgtc
    13621 agtcttccct atgctcaggc aagcaacggg gaatactcgg tattggcgca aatcgttctg
    13681 acctacgatc ccaacacggg aagtgtcaca aatgccgtgg ccaacacttt ggctttggat
    13741 gatctcggag gaaccaacca ggaaatcaac cagatcctga acgattattc caccaggacc
    13801 gattatgtcg tttttgtcag tgaaatcctg gccgatagcg attccgacta taccaggtat
    13861 aatctcgctc cttggggagc atccgttttg agagattatg tatcgacaga tgtcggtgtc
    13921 ttaaacgccg gcgggttcag ggttgacatg gaatacggta atgtcacgat gggcgatctg
    13981 attaccatct atccgttcga taattatatc aaaacatgtt atctcaccgg cagtcagttg
    14041 attggtcttt acgaggattc attaagcccc aatatcgatt tggtcttcga cgacaaagtg
    14101 acctattccg gcggaacgct gtatgtggac gggatagccg tccaggcctc tgatctttat
    14161 ctggtcggcg ccgtcgatta tgtttttgac aaaagcgcct atgtgtttct cgacggaagc
    14221 caaataacca cgacatcata tctgatgcgc gatctcctgg caaccgattt gagaaaccac
    14281 ccgtattccg gcggattcaa cccttcggac ggcaccgcca tgactctcgg gtcgatttgt
    14341 tatgattttc gcaattttta tctttccaaa gaagaaacgt ttgtgatata atcttatatg
    14401 gctgcggaaa acaatcccaa atttccgcac taacgggact gtagctcagc tgggagagca
    14461 ccacaatggc attgtggggg tcgagggttc gagccccttc agttccacca gtcgaaatta
    14521 catccgcccg gcgggcggat tttcttttcc cgccaggtgc gccttgtcat aatccgtaac
    14581 aagatttcat cgccagtagg agtgtgatcg aattggcatt atcaatctat atcaataagc
    14641 ataccaaaaa actaatcatc ctgatttccg tctcggttct ggcagccctg atgactgcgt
    14701 ttcttattta tgcgagcgtc ttttaccgtt ccgaagtgaa cgtggcggaa atcacgaacg
    14761 tgacggtcga aaaggttaat gagaattatt acctgacacc ggaagaagat ttcgaaatcg
    14821 gaatcatttt ctatcccgga aacaagatcg cggccgaaag ctatcttccg tttctttcca
    14881 gattcgctga attaggcatc ctggtcgtgg tcgtggacat gccttaccat ctgacgttgt
    14941 tcggcaagaa caaagcggct tcgatcatag ccagctatcc ttccgtcgat ttctatatca
    15001 tgggacacgc cgaaggcggt gaagccgcag cgagatatgc ttcgaagaat cccgacctcg
    15061 tcaaaggtct gatccttttg gcttcctata gcaaatatga agtgagcgtt cccgctctat
    15121 cgatctacgg cacgctcgac accgtcatgg acatggatga atacgatgcc aacgaagcca
    15181 atctcgtctc ctcgacgacg attcccctaa tcggcggcaa tcattcgatg tatggcgact
    15241 acggcctcca gaagggcgac acagaaacaa ctcttaccat taccaccctg cgtacggaaa
    15301 tcgaagttgc cgtgagggca ttcatcgcgt tgaccgcgtg attacgcttg ccgttttatc
    15361 ctttcggggg taaaacagtg ttcgcgtggg ggcgtagctc agctgggaga gcgttagctt
    15421 cgcatgttaa aggtcgcggg ttcaaacccc gtcgtctcca ccatatataa atgaaatagc
    15481 ttccaaaagt tggagaaatg gtatactgaa aataccactc tgatgaaggg agctattttt
    15541 attttggaac taacactata gcggaaagcg aaaatgcgcg aaaagcttgt tgtgaaaaaa
    15601 acacgtaata acgtgtcaga aatgtatgat ggatacgaca ttgatcgagc tttattgaat
    15661 cttcaaataa gatttgaatt ctgcttttgt tttttgtttt aaagtataac agagtgttga
    15721 tagcatatat gtaattgttg gtcgcttata tataattaca ccctaaaggg tataatgtca
    15781 gattaaaaac agtatattat accctaaagg gattatatat ataatattat attgacatta
    15841 gtcccgaaag ggtataatat ataactataa ataactagca aaggagagat catatgaaca
    15901 agatagctga atatataaaa aaacaacgca aagcagcaga gttgacgcaa gaacagttcg
    15961 ccattcgatc aggtcttggc cttcgcttta tacgggaact ggaacaaggt aaggaaactg
    16021 tgcgcttgga caaagtaaat gtcgcacttt ccatgtttga tatggaagcc gtgccgggta
    16081 agaaggagaa atcataatgg gtgctttcag aaaagcgtat gcatatgttc gggactgctt
    16141 tgccggtgtt ctagaggaaa ccgatgaagg atactctttt tcttatgatc cgaaatacat
    16201 agaaagcgaa aacgcggtac cggtatccct gactttgccg attcaaaacg aaagtttcat
    16261 ttcaaggaca ctgttttcat tttttgacgg attaattcca gagggatggc ttttgagtgt
    16321 tgtcactcga aactggaaaa ttcctgaaaa cgatcgcttt gcattattac ttgtcgcatg
    16381 caaggatcca ataggcaatg tcagtatcaa ggaggtgtgc gtatgaaatg tttatgctgc
    16441 ggaaagacct taaaaactga aaacgaatcc ggttggcaca agagctgtat tcgcaaattc
    16501 tttggaacta cgcagatacc ccaaatagat gttgacgaca acacattgga gacgcttgcc
    16561 actatgagtg ttgacaaggg ctacacagtt cccggtgttc aaaagaagct ttcattacat
    16621 ttgctgtcgc aaggtggtac gccaagactt actcttgtag attaccctgc cggatacatt
    16681 ttaaaacctc aagttgagca atttgaggca ctaccggaat cggaacacct tgtgatgtgc
    16741 atggcggatg ctgtcggtat tgcaactgtt cctcatgctc tgattcgaaa tggaagcaat
    16801 tatgcatata tcacaaaaag aattgaccgt gtcttatata aagactttgt agaaaaacta
    16861 gctatggagg atttctgcca acttgattta agattaacac aggacaaata tcacggatca
    16921 tatgaacagt gtggaaaaat catcaaaaga ttttccgaaa gaagcaaact tgatttatcc
    16981 gagttattta ttcgcattgt ctttttctat atcactggaa actcagatat gcatttgaag
    17041 aatttctcat tgattgaaac ggaagtaaga agcatgaaat atgtgctatc tgcggcatac
    17101 gacctcttgc ctgtaaaagt aaacatgccg gaggatagag aagattttgc attggccatg
    17161 aatggcaaaa aaacgaatat ccgaaagaaa gacttctttt tgtttgccga gaatataggg
    17221 attaataaag aaataactca aaaaatgata gctcagttgg ttgctaaaaa agatatgctt
    17281 cagtcaatgt gtatggaatc atatctgcct gaacatctga aaacatcatt gtatgaattg
    17341 attgaaaatc gatgttctgt gttgatgagt tgagttgaca taatatttct tcatatagtt
    17401 aaactctcct ggtaagtcaa aacgaaatga tattattaat ccagtaattc ttaccgttaa
    17461 aattatattt gcataatttt atcggaatag gaaatgctaa cccaattgaa aaacggattt
    17521 caacgttatt gttggaatcc ttttttatag tttattccct tgagatttgt tttgaaatat
    17581 cttgccaaaa aatatcctga catgatagaa tatgtctaga agatttgcat ctgcaaaagc
    17641 tccgataaag ctatctacag tacagtctga acaggcagtg ccggcaaagg cacgtggccg
    17701 ggccgttagg cagcggaatt ccaagggatt ctgtgggaca gaaaagcccc cgggagccct
    17761 tttttatttt cctttttttg gaggtccgcg atttatgaaa aaaaccggct atgaactgat
    17821 taagaaagtc tcatggtggt cgattgccgt caacctgttt ctatccgctt tcaagatgat
    17881 tgcgggaata ttggcaagaa gcagcgccat ggtgactgac gccgtgcatt cggcatccga
    17941 tgttctgacg acattcgtcg ttttgatcgg cgtcaaactg tccaaaaaag aagcggacgc
    18001 cgaacatcct tacggacacg aacgcctgga aagcgttgcc ggcatgatcc tggcgatctt
    18061 cctcgccttg accggcatcg ggatcggcta ttccggaatc atgaaagtga tatccggaat
    18121 cgaaggaacc ctcgctgttc cgggagtgtt ggctttggtt gcggccatcg tgtcgatagt
    18181 tatcaaagaa gccatgttct ggataacgag aaatgcggcc aaaaagacca aatccacggc
    18241 cctgatggcc gacgcctggc atcaccggag cgacgctttg tcttcggttg gcagttttat
    18301 cggtatcgga ggagcgatgc tcgggcttcc catcatggat ccgatcgcca gtttggtgat
    18361 ctgccttttc atcctgaaag ccgcattcga tgtcgcaaga gacgccacca accggatggt
    18421 cgaccgctcg tgcgacgaag cgacccagtc caaaatgaag gaaacgatca gcgccgttaa
    18481 cggtgtcaaa tcaatcgaca ttctgaaaac cagaatcttc ggcaaccgga tttatttgga
    18541 cgttgaaatc gggtgcgacg gcacgatacc tttgatgaaa tcgcatgcga tcgcggaatg
    18601 cgtccatgac aggatcgaag agaccttccc ggacgtcaag cactgtaacg tccatgtgaa
    18661 tccctatcta gagaagaagg aatgaaaaaa gccggctttg tgccggcttt taatttactc
    18721 aaacggttat gggactgatg ttgtattttc tgacgaagaa ttcgccgttg gaataacgaa
    18781 gataattgat gctgcaattg gaaagataag aagcataggt gaaaccgggg acaagcctta
    18841 cgagaatcgc cttgatcaca tgcgaatgcg ttacgaccat gatctttttg ccgggatact
    18901 tttcgcagat ttcctgcaaa gtctcgaaca ccctgagctc cagatcctcg tttttttcca
    18961 tattcgggat tgtgccgtgt ttgatgaggt cggcgtattc gtcggtaatc ttctggccgt
    19021 cgtaatcgcc aaaattcctt tcgatcaaat tctgatgcac gatgatcgat ttctttgtct
    19081 gcatgccttc aagtatcagc cttgccgtca tgaaagcccg cttcagggga gaggaaaaca
    19141 ccagatcgat cttttctttt ctctgtctta gaaattttcc cgtttccaga gcctgtttca
    19201 tgccgtattc gttaagggga ttgtcgaaac ggccttggac gacataattt cgattggcgt
    19261 ccgtttcgcc gtgtctaatc aaaatcaatt cattcattca aaatcacctt taacaattat
    19321 actatattat gcttgaaatg tggtataatt tttttgcgag gtgatgccgt tcatgagaat
    19381 ggttgacata attgaaaaaa aacgcgacgg attcgaactc accgaagaag agatcgactt
    19441 cgtcatatcg ggctatgtca agggaacgat tccggattac cagatcagtc cctggctgat
    19501 ggcgatctat tttaaaaata tgaccgacag ggaatcgagc tatctcacca aggcgatgct
    19561 ctatagcggc gatgtgatcg atctaaccgg gattaaaggg atcaaggtcg acaaacattc
    19621 gaccggcggc gtcggcgaca agacgacgct ggtgctcgct cccctggtcg catccatggg
    19681 tgcgaaaatc gccaaactgt ccggaagagg attgggacac accggcggta cgctcgacaa
    19741 actcgagtcg atcgacggct gccgcatcga tctatcagaa actgccttca tcgaccaggt
    19801 caacagaatc ggaatcgcag tcgcgggaca aaccgccacc ctggttccgg ccgataaatt
    19861 gctttacgcc ctgagggacg tcaccggaac ggtgccgtcg atccctctca tcgcctcatc
    19921 catcatgtcc aaaaaactgg caagcggagc cgatgtcatc tgcctcgatg tcaaagtggg
    19981 tgacggcgcc ttcatgaaaa cgacggagga tgccaggaca ctttcccggc tcatggttga
    20041 aatcggcaaa tccttcggta agaaagtcta tgcttttctg accggcatgg aggaaccgtt
    20101 aggcctggcc atagggaacc gtctggaagt cagggaagcc atcgacacgt tgtcgggaaa
    20161 aggcccggcc gatctcgagg aattatgcgt agccatcgcg tcctacatgg cgtatagcgc
    20221 caaagcggca aagacggtgg aagaaggcaa aatactggca catgaaaatc tatacaacgg
    20281 aaaggctttg gccaaattct acgaattcat caacgcccag ggcgggcata tcgatgattt
    20341 ggaaaccttc gtcaaacccg agggcatcat gccttatgtc tctagaaaat ccggctacgt
    20401 gcaaaaaatc ctggcccaca acatcggact ggcttcgatg atgctgggcg gcggccgcgc
    20461 gaagaaagaa gattcgatcg acccgatggt cggaatagtg ctaaaaaaga aaatcgggga
    20521 aaaggtggaa aaaggcgacg ttctcgcaaa catatactat gacaaaccgg tatccggcga
    20581 ggtgatggaa gaacttgcca cggcatttca aataagcgaa gaaccggtga attctcccaa
    20641 agtcattatg gagataatcg gtaaggagta atgcttgcat aaaggacttc tacgtgttat
    20701 aatcaaactg gaaaaaaggg agctcgggga accgggctga gagtataact agaccgttat
    20761 agaccttacc tgatctggat aatgccagcg taggaacata gattgtccca aggtcccctt
    20821 tttccgggac cttttgtttt tactatacaa ggaggaattg tcatgaaaag aaaccagact
    20881 ttgctagctt tgttggaaac cgggatcatg gtggcgatag cggtgatcct cgatgccatc
    20941 ttcggagcca tttacacctt cccattcgga ggatcgataa cgcctgccat gtttccgatc
    21001 tttttcttag cggcgagaag aggcgtgaaa tacggcttgt tgggaggatt gctatacgga
    21061 tccatccaac tgatgttctc cataccctat attctgtctt tgccgcagtt cttgatggat
    21121 tacatcgtct cattcaccgt tctggggttc tcggggctaa ttcccgggca actcgagaag
    21181 ggaggcagat ttgttttggg aatactcttg ggaaccgtgc tcagactgat atcggcttcg
    21241 atcgccggcc ttctgttctg ggtcgcctac attcccgaag aactcgactt catgcacacg
    21301 cttttcggcg tcgatatcgc cggctttttc gcttccgaca atgcggcggt gcttttcggg
    21361 gcatttgcct ataacatgct ttacctcgtt ccctctttca tcatctgcgc cctgatcggt
    21421 gtaatcttcc aaaaacgcgg ccttctcaag ttccggttgg aaggatagac agaatactgg
    21481 cactcaatgc ttgacagtgc taattcttgt ggtataatag atacgacaag attaggaggc
    21541 attgcaatga aagaaaccca tgaattcaaa accgagtcga agagactctt gaacctgatg
    21601 atcaattcaa tctatacgaa caaggaaatt ttcctgcgtg agcttatctc gaacgcatcc
    21661 gacgctatcg atcgctacca ccttctgagc ctgacggacg acaagctcga gaaaaccaac
    21721 gactacgaga tccatctcga cataaacaag aaacttcgcc ggctagtcat caccgacaac
    21781 ggcgtcggta tgaccaggca ggagttagaa gacaatctgg gtacgatcgc caaaagcggc
    21841 actttggaat ttttgaagga aatgaaggaa aaagaagaaa aagacaaaca aaccgttggc
    21901 ggtatcgatc tcatcggcca gttcggcgtc ggcttctatt cagctttcat ggtgtcgaaa
    21961 aaggtccagg tgataaccaa atccccgttt gacgagaaag cctggaaatg ggtgtctagc
    22021 ggcgaagagg actatacgat cgaagaatgc gacaaaacgg aacgcggcac cgaaataaca
    22081 ctatttatcc gcaaaaacga agacggagaa aactacgatg aatttctcga agactacaat
    22141 cttcgttccc ttgtcagaaa atattccgat tatgtccgtt atccgataac gatggagaaa
    22201 gaaaccaaaa agccgaagct tgacgaaaaa ggcgaagaaa tcgaaggtca gttcgacacc
    22261 gtgattgaaa acgagactct taattccatg acaccgatct ggaacaagaa caaatcggaa
    22321 gtcaaagatg aggaactcca ggaattttac aaatcgacct atttcgatta ttcggactat
    22381 cttatcaata tctggacaaa cgtcgaggga gccctgacct acaaagcgat ggtcttcatc
    22441 ccttccaaag cgccctacaa cctctattcg gaaaaatacg aaaaaggcct tcaactctat
    22501 gccaaaggcg ttttcgtgat ggacaagtgc aaggaactcg tccccgatta cctgcgtttc
    22561 atcaaaggac tcgtcgtcag cccggatttt tccctgaaca tttccaggga gatcctccaa
    22621 cataaccgcc agcttgccaa gatcagccaa aacctggaaa aaaaggtttt gtccgaactg
    22681 gaaaagaccc tgaaaaacga ccgggagaaa tatgaaaagt tctggaagga attcggagtt
    22741 aacatcaaat acggcattta tgacaacttt ggcgaaaaga aagacatgct caaggatctt
    22801 atcttgtttg aaagcgtgaa aagcgataag cttttgacct tgaaagaata cttagaagcg
    22861 atgcctaagg accaaaaaga aatttactac gcttcggcgc caaaccgcga ggccgtcaaa
    22921 gtgtctccac aaatggacat tctcaagaag aaagactacg acgtgctggt cctaaaggac
    22981 gatgtcgacg aattcatgat ccagattctc cgcgaatacg aaaaactgga attcaaatcg
    23041 atcaaccagg gagacctcaa catcttagac gacaaggaaa aagaggatct caaaagtttg
    23101 gaaagcgaaa acaaagatct cttggaaagc ctaaaagaca tccttggaaa cgaagtcaag
    23161 gacgtcagac tgtcgacaag actttcggag tctcctgtat gcctcgtatc cggcgaaggc
    23221 ctctctttcg agatggaaaa ggtcatcaac cagatgccag gcgaaaaaga cgtcaaagcc
    23281 gaaaaaatcc tggaaatcaa tccgaaacat gaacttttcc aggcgatcga aaaagtgtat
    23341 gccgccaaca atgacctctt ggaggattat gcctgggttc tttacaacca ggcgctcttg
    23401 atcgaagggc ttcccgtcaa ggatccggtg tcgttttccg acaagatggt ccgcctgatg
    23461 gtttacgcgg ccacgggcaa aaaagatgaa taaactattc aggcagttga aaaaaggtct
    23521 gaagaatact ttcaggggat tcaagaccaa aataaaaacc gacggagacc gttatggcga
    23581 agatttgaaa atcgccgtct ttgctcccgg cggtaggaac aacgcctgtt tccgtttccg
    23641 ccatgaccaa gcgtccaaca atctggccgg gttcatggac atagtcgaat gggatggccg
    23701 caaggagaca agccgacgca gttattcgtt ttccgacgtc acgcggatgg aaaatatccc
    23761 cggattttcc gaagaggaaa tcaatctact gaaaaccgca tttctctata cccgcgaaaa
    23821 cgataaaatc gtcacagtca tcgacccgca aatccaggct caaatataca tagaagccat
    23881 cggccaggca tttcccgaca tcatgaggga aatcatcgga tagaaacaaa aaagggacag
    23941 cgtccctttt tttctagaag tatactttat cgacaacgga aagataatcg agtcttttca
    24001 cataagcttc cttcagctca tgcccataca tttcgaactc atcgagcgaa atcgattttt
    24061 gcccgtcttt cgatctttca tagaactcgc tgacatattt gctatcgagg agaaaagacc
    24121 ttccgagaag cgagaaatgg acgatgatga aactgattcc cccgtgcctt tgcacgttat
    24181 ccagatgcgt gatctggtga atgtggatat tcttcagggg gaaagaggtt ttcgatctcg
    24241 tttccttcgc ttcgaagtcg atgtatttcg ccttgtatat cccgttgtag tcggtagtgg
    24301 aaggcacttt gtaatacgcc tcggatatcc gggcggcgct tcgtttttca taatcgactt
    24361 tgacaacctg gatcggtatc ggtttcttgt agatataggc gatgtcgttg tcgatataat
    24421 actgattggt gtcgttgatg gctttttcca ggtccatccc ccggtttgcc gtggatgttg
    24481 cgacatgggc gcccgactgc cttttgatcg gatagttcat catatcaccg cttttattct
    24541 accacagttt tgaaaatccg ttgacaaaac ggtttgaaaa aaaacaaaat gaatccatcc
    24601 cggacgggat atgcaaaaat actcccaaaa aatgcgcgta tcgggtataa tattatttaa
    24661 caaggaagga atgatcaaat gagtcagatc agattctatg ccctgggcgg tctcggggaa
    24721 aacggaaaga acatgtattg cgtcgaagtg gatggcaaga tcgtcgtcct cgacgccggt
    24781 ctgaaacacc cgaccggcaa ccttttgggt gtcgatgcga ttgttccgga catctcatat
    24841 ctcgaaagcc gatcggacga tatccttgcc gtcatgctgt cgcatgccca cgacaagaac
    24901 atcggcgcct tgccgatgct tctcaaaaga ataaatgttc ccgtctacgg aaccggattc
    24961 gccattgccg tcgcgaaaga ccttctgacg gaaaatagga tggatccggc aaagtatatc
    25021 ttcaacatca taggcgaaaa cgacaggatc gagtttcccg gtttttccgt cgaattctac
    25081 gccaccactc attcggtgcc ggaatcggcc ggagtggcca tcttgacaga agacggagcg
    25141 attgtctatg cgaccgactt cacgtttgac caaaacagcg gttctttcta ccagaccgat
    25201 ttccagaaac tgaccgcttt caacacccat ggcgtcctcg ccctgctttc ggaatcatcg
    25261 ggcgccctgt cgctcgggca ttcctcgacc gattataatc tcaccatgct gataagggaa
    25321 accttccaga gagcaaaaca ccgggtgata gtcgccatgt attccaccga attatcgaac
    25381 atccagaaag tcatcaacga agctctggcg gcgggaaaga aactggcgat catcggacgc
    25441 aaggcccaga gactggtcga catcggggag aatctaggat atatccagat ccccgcggac
    25501 aaacttgtca atctgcgctt tatagacgac gagaaccaga acgacttcga cgacgtcgtc
    25561 tttctcgtga ccggcgagaa acacgaacct ttcttcatgc ttcaaagaat ggccaaaggt
    25621 ttcgaccggt tgatccatct gaaaaaggac gataccgtcc tgatgatgtc gtatccgata
    25681 gtgggaaccg aaaaaatcgc cgccaaaact tacgacaccc tgttcaggat ggaagtcaat
    25741 ctaatccggc cgaacaagaa gatcctgcca ttcttccatg catcagccga ggacatcaaa
    25801 ctgctttaca cattgctgaa gccgaaatac atcgtgccga tcaacggcga attccgccat
    25861 cagttagccc agcacgatct tgccatcgaa tacggatatt cccacgaaca gattgccctg
    25921 gtcgacaacg gcgagaccat cactttcgaa aacgggattc tcatcaacca gcacgacatg
    25981 atcatgaacg gctctttgct gatagacggc aatttcgaaa ccgacatcaa cgacgccgtc
    26041 ttgaaagaaa gagaacttct cgccgacgac ggattcctga tgatcgtcgc caatatcgat
    26101 tcccgggaac gcgtcatgaa aaacgaaccg gaaatcgtca gccgcggatt catgcccctg
    26161 aaaccggctg acgacacgta caaacagatc gtcgccattt acaaagatgt cacaaccaaa
    26221 tcattccagg aagccaattt cgattggaaa gtgtaccgcg aacaattccg ttacgaagtc
    26281 cagaagtatc tgtacggaga atccggccgc aaaccggttg tgatcccgat ggtgatcgat
    26341 gtgtcggaag acagaccctg caaagaaata taaaagcgga tttctccgct tttttatttg
    26401 ccttcgtcga tcgctttcct gagtttctcg tattgttcct tgaaagcttt ttccagcttg
    26461 gtgtgatcct ggggaacata gtatctggtt ccttccatct cggacggaag atattgttgc
    26521 ttgacgatgt ggccgggata atcgtgagca tatagataag gcgacttttt gtcataggtg
    26581 ggattgttga tgagatggct cggaaccttg taggtcttgc cttcctcgac atcgcttatc
    26641 gctttgttga cagccagatg cgcgctgttg gatttcggag aaagacacat ttcgatcgta
    26701 attgctccga gcccgttgat cgcttccgga aaaccgatgt gtcttgcaat atcgcacatg
    26761 gcagctacct tgggtcccat tccgggattt gcgagtccga tatcttcata tactatggcg
    26821 cttaatcttc gaagaagcga ttccagatcg cctgctacaa tcagcctgga aaggtaatgc
    26881 aaagcagcgt cgacgtcgct tccccggact gatttgtgga aagcgctcag aatatcgtaa
    26941 tagttgtcgc ccatgtaatc cgcggataca gacgcttttc cgagtacatc tttggcgaaa
    27001 gtgatgttga tttcattctg tttttttccg gcttggatca taagcatttc caacatattg
    27061 atagcagctc tgatctcgcc gttcgcatag atggatatga aacggtacac atcgtcgttt
    27121 atcacgactt cttttccaaa agaagaaaga tcgatcttgg ttatgtaatc gtatatttcc
    27181 gtttccgaag gcctttcaag cttgaatatc gacacccggc ttcggattgc cggattgacg
    27241 gatatatagg gattttccgt tgtcagaccg accataacga ttttgccaga ttcgacatat
    27301 ggcaaaagat agtcctgtac atcccggttc atccggtgga tttcgtcaac gacaagaagc
    27361 gtgttggcgt atagttttcc cgcttcgaca atctgtttta aaaccgattt gggatcggag
    27421 gatgcgttga acttgaaatg gttcaacgga taatgatcag caaccaataa tgcaatcgtt
    27481 gtttttccga ctccggttgg accatacagg atgattgaaa ccattttgtc ctcatccagc
    27541 atctttttta aaaccctgac gattttatcc tgtccgacca cttcgtcgaa tgttttcgga
    27601 cgtaatctgc tggccagcgg ttcgtacatg ttttcgcccc cttccgtcta tagacaaatt
    27661 atatcacaaa cttgttaaaa cgcccaaaaa agcatataat attcatgatg gaaaacgaag
    27721 ggacgatggg acatgctaaa agatgatatc ctggttttga agaaagaaaa gaacgcggtg
    27781 atccttgccc atttttacga gcgaccggaa attcaggatg ttgccgatta tgtcggagat
    27841 tctcttgcgc tttcccggat agcggctgat actacggcag acatcattgt tttctgcgga
    27901 gtgaatttca tggcggaaac cgccaaaatc ctttctcccg acaagacagt tctgttgccg
    27961 gtgaaagaag ccggatgccc acttgcattg accatcaacg ccaacaaact gcaaaaatac
    28021 aaagacgaac atccagatac cgtcatcgtc tgttatgtca attctccagc cgaagtcaag
    28081 gctttgtccg acgtttgcgt cacgagctcc aatgccgaga aaatagtatc ttccttaaaa
    28141 ggaaagaaaa tcctttatgt ccctgataaa aacatggctg gctatctaaa agataaactc
    28201 cagatagaac tcgatatctg gcccggtttc tgtccggctc ataacagaat ctctacggat
    28261 gatgtcatca agaccaaaaa aagatggcct gatgccaaag tcctgattca tccggaagca
    28321 ccgcttgagg ttctcaagct cgccgactat gtcggtagca caaaaggaat cctcgattat
    28381 gccaaatcat cggacgctac ggatttcatc atcgggaccg aagaagggat catctatcct
    28441 ttgacaaagg aaaatccgga gaaaaaattc cacttgttgc catacgggtt gtattgccgc
    28501 gacatgaaac tgactcatat tgatgatgtg tataacgctc tcaaaacaaa caaacatgag
    28561 atccagatag acgaaagcat ccgaaaagcc gctaaaaagg cacttgacaa gatgctggaa
    28621 atgagctgat tctttatgaa caaaaaagaa gccgatgtac taatcatagg caccggactc
    28681 gccggtatct ataccgccct caacatcaac agagatcata aaatcatgat acttgccaaa
    28741 tcttctctgg acagttcaaa ttcgatgctc gcccagggag gaatagctgc cgaactgaca
    28801 gacgatccca tcagactgga tagccatttt gaagacacat tgaaagccgg atcatatctg
    28861 aatgacagag aagcggtgga atatctcgtt aatcatgcca aggaagccat cgaatcattg
    28921 gtcgctttcg gcattcattt tgataaagac gataacaatc attatctttt gacaagagaa
    28981 ggcggtcatt ctcgcaatcg gattctccat agcggcggag acatgtctgg ctattacacc
    29041 accaaaggcc tttcggacct tttgaaagat tacacgaaca tcaccgtcaa ggaacacaca
    29101 atggcgatcg atatcatttt taacgaagaa aaagaagcga taggcgtcaa agttttggaa
    29161 aaaggcgtcg gatcatatcc gatatttgcc aaagccatca tcctggctac cggtggaatc
    29221 ggttcaatct acggagccac taccaacgat ctttcggcta ccggtgatgg catcgggatt
    29281 gctataagag ccggagccga agtgaagaac atggaatttg tccagtttca cccgactgct
    29341 ctttatcagg agaacattga aaacaaacgc cgctttctga tttcggaagc cgtacgcgga
    29401 gaaggagcca ctcttctcaa tatccgtaag gaacgcttca tgaacaaata cagtcccaaa
    29461 ctaaaggaac tagccccgag ggatatagtc tcccaggcta tctataggga aatgtatgat
    29521 acatggaccg atcatgtcta tcttgacacg agaaatctgg atccggaatt tctcaagaaa
    29581 cgttttccca ctatttacag aacttgcaag gaagccggat acacgctagg ggtagatctg
    29641 atcccggtgg ctccttgcga gcattttctt tgcggcggaa tcaaaacaga tcttcatgcc
    29701 agaaccacga taaaaaacct gtatgccgtg ggagaaaccg ctatgaccgg agttcatggt
    29761 gcgaaccgcc tggcttccaa ttccctgctt gaatgtgtcg tcttcggaaa aacagcggct
    29821 gatatgatca acaaaacaat cgattcaatc aagattgaat cttattttga aagcgaagaa
    29881 atgccggcat acgattacaa ttaccgtccg atcagaaaga agatcagcga ctacatggac
    29941 gaacatgtgg gaatcgtcag aaccagcgac ggtttgaaac tcgcggagaa gactttcgca
    30001 tcgatcatgg aaagcctgtt gaaatccgta aacatgacga gatactacta cgaaaccctg
    30061 aacatggtca cgacagccat cacgatcacg agacaggcgc ttataagaaa ggaaagcata
    30121 ggatgtcact tccggctgga ttagtcaaaa ccaattatca tacccataca tatctttgcc
    30181 gacatgcgaa cggcaatccg tccgactatg tcaagaaagc catcgaactc gggttcgagg
    30241 cgatcggcat ctccgatcac gctccgttct ccgaactggc tgacagaagc atccgcatgc
    30301 gtccggacga atatcccctt tatcttaagg aactgaagga aagcatcgac agatacaaag
    30361 attcgataag aatatacaaa gggcttgaaa tcgaatatct tccgaataag ctggaatatt
    30421 acaaacaagt attatgtaac gatctcgatt atctcgccct ggggcaacat tatgtcccgg
    30481 atccgaagag ccccgacgga ctgaaaagca tttacgatct tgataacata gatcagataa
    30541 gaagttatac ttatactttg acggaagcga tggataccgg attattcaaa tt
//
LOCUS       DAOPJL010000013        29753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1646_length_29753_cov_8.056300, whole genome shotgun sequence.
ACCESSION   DAOPJL010000013 DAOPJL010000000
VERSION     DAOPJL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 29753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29753
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1646_length_29753_cov_8.056300"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLP02_02910"
     CDS             complement(<1..443)
                     /locus_tag="PLP02_02910"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPF42435.1"
                     /translation="MPFSHEITHICKQTGARCGILHTESKDIPTPVFMPVGTQATVKT
                     LDSTEAREISEGLILANTYHLWLEPGEKIIAKHGDVKKFMNWDGALLTDSGGYQVFSL
                     SKIRKIQEEGVTFRHHLSGELLHMSPEDSIRAQNVFGADIIMSFD"
     gene            complement(448..1017)
                     /locus_tag="PLP02_02915"
     CDS             complement(448..1017)
                     /locus_tag="PLP02_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HPF42436.1"
                     /translation="MDKKILVEVSARHVHINEKDLETLFGSGYKLTAKKMLSQPGQFA
                     CEEKVTVIGPKKEMNLTILGPTRSKTQVEISLTDARTLGITAPVRESGDIAGTAPCRI
                     VGPKGFIDIKEGVIVAKRHVHLTPEVASLYQVADKDIVSVRVDTPERSLVFGDVVIRV
                     RADFSPAMHIDTDEGNAAGISGEVFGIIL"
     gene            1173..1856
                     /locus_tag="PLP02_02920"
     CDS             1173..1856
                     /locus_tag="PLP02_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPF42437.1"
                     /translation="MMNELLWFAFALANFILFLGMYKLFGKTGIFVWVAISTIIANIQ
                     VTKNITLFGVEATLGNITYGTIFLATDALNEIYGKKSAQKAVLMGFYVMVATVVLMQM
                     AIWFIPSTSDLNNDALRSIFGFFPRLLGASLTAFLISQFLDVHLFQKIREKLPSDKFL
                     WVRNNGSTFISQLVDTAIFVPIAFLGVYSGSVLLQIGLTTYLIKVIVAMLDTPFLYIC
                     KRIIPFDNN"
     gene            complement(1882..2907)
                     /gene="queA"
                     /locus_tag="PLP02_02925"
     CDS             complement(1882..2907)
                     /gene="queA"
                     /locus_tag="PLP02_02925"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016201680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPF42438.1"
                     /translation="MKTSDFDYILPKNLIAQSPLPNRTDSRLLMVNRTNFNLEHRHFR
                     DLPQYLEKGDVLVLNDTKVMPARLIGEKPDTGAHIEILLLRQMEDDVWETLAKPARRI
                     KKESIVSFGQGLLMAKCIEEKDDGIRKMRMYYKGVFPEILDKLGETPLPPYIHEKLED
                     KNRYQTVYSRELGSAAAPTAGLHFTKALLDEIREKGIETVFLTLHVGLGTFRPVETED
                     FTKHKMHSEYYVFDTEAAKALNKAKTEKRRIIAVGTTSTRTLESVYAKYGEFRPDSGY
                     TDIFIYPGFQFRAIDGLITNFHLPKSTLMMLVSAFSSREIIFNAYREAIEREYRFFSF
                     GDSMFIR"
     gene            complement(2908..3912)
                     /gene="ruvB"
                     /locus_tag="PLP02_02930"
     CDS             complement(2908..3912)
                     /gene="ruvB"
                     /locus_tag="PLP02_02930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPF42439.1"
                     /translation="MDKRIISNNLLMDDEIEETLRPQMFSEYIGQTNVKDMMEIAVKA
                     ARKREESLDHVLLYGPPGLGKTTLAQVIANELGVQMKTCSGPTIERTGDLAAILTSLE
                     PGDVLFIDEIHRMPKIVEEILYSSMEDFVIDIVVGKDSGAKSIRVDLPPFTLVGATTR
                     FGDLSGPLRERFGIVHRLDFYTDGELETICQRTSRIYDCPIDSQAVIELAKRSRGTPR
                     IVNRLFRRVRDFADILGDGTITLDITRKALEKLKIDECGLDRKDREYLSTIIEKYKGG
                     PVGLDTIATSISEDPQTLEDVYEPYLIQSGYLSRTPRGRVATEKAYHHLKKMYQESLF
                     "
     gene            4038..7499
                     /locus_tag="PLP02_02935"
     CDS             4038..7499
                     /locus_tag="PLP02_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42440.1"
                     /translation="MAKHRDIIATYLHDFPEIKKAFQSAQEQLERRLSMVKEDNAHEL
                     ADIENSYKTYEDDHLHKYIDKNDEFKENIDFINAEYRELLDDVESDISLNNKKTEELI
                     EAETKEFENILNDIDSLKQEAGDQYNEMTRMIGEKIDHEMHVHHEFIAQENAKFLSTQ
                     EHYQEINSDQANRLMWAIEQSKNALADLSSRLEEKSSQHAAFMDESATEILHSLRETK
                     DRVNSLFKGTTDLFSEQKHHIEQLNHERQKPHSQLNQTIVRQYVKKIREVNQKKIAFD
                     QMIRKELDSSLEVIGERIIKFDAEKNRLETEKAILQYEIVKKKADYLLNRNKLIADSL
                     ISKYQNEIKKIKIDSFRRVEEIKLAYYMPAAFFQNSINLYSNFAFYVNESFTDLDMLL
                     SDLTMTNKRITDLETNYVTHSAKAVEDYKLRVMAQINEVTGKLTGLITKIDTLSREIV
                     TLESRNQLEIAEIRKKMESTDIMGDYQKHLAKLENDEYFACYQHDINLNKIKAANRCD
                     GELLGIEQSVIKLHRDREMIDVRERQAKAMSQNEKEIRDLAYDKALAIFRAAYDRNKL
                     ASDIQNQIESEKVEFSFKAHNYLFARKYEELRNKHHRSEADGSEGVVEYVHNAQKMID
                     ANNNELKDFIAGIAQGRNARDYAYYLETVKTNMIMALKAELKTKTEKNRKAIDLYKEH
                     FQRAHELLQGAFADISHQIKQHLANLDENMALVQANMIIRDHAYLHQIADIFANAYDD
                     AFSFIKGISSKADPSLLEKQKESALNNYVILADITLTALQKPKISAKKTNSVLEHYYV
                     TTIDLINDSINTVTAFYADMEIFVKKNDISFIKKAEENEATSEKMITEVYDELIVKAA
                     RMGNDSDKKVNSMKKITTSIESELKRKVAKVNSLYINNMKTEADKLGYISKSFEKIMS
                     DNERALATYRISETARIKREGADREKAYGKFIRDYERQKAASEETCRLEGDYSALSAR
                     KGVSDIESMLKEYEEKTKLLPKERDKSIIALESGKETKIMKQNTALMQQLAIIEGNKF
                     SSRPKFMKEIEAIKDRLPTDYLHLYQEIQKAESDLLQQYIETETSISRDFQLFLSGQE
                     SHKRLLSDEKLVYSPFEGFLDLQDNLIEKAAGAYETAIDKSDLATKKIKETENNSKEK
                     QDRIINE"
     gene            7520..10453
                     /locus_tag="PLP02_02940"
     CDS             7520..10453
                     /locus_tag="PLP02_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42441.1"
                     /translation="MAEEKNQPKTGNTNSEAYLEKSRQSYEKEMALFEQFYKNISHRA
                     DTHRQITVNNVNEVEKRLTSLKEEADKLRDSIFYHDETVIVDRQEIIAKTEDDVHQNN
                     SLLLANDRYDAENLVSTVDYMNKALIQVKLDFFDAYNNSYLKSIVANDDFTALFEAKS
                     ETFQKVLDKHQKNVMSIFLDLNEEIREMDDKIARIMHEKNDTISSIVQFYDQESKNYV
                     DNQLMFSPASDPTSIDIQALVSDKINQFHTFRRHLEEQTANVEKHIVGNFTDLDHQIV
                     GKMEKHIGNTMLNNSDFFNSPEDYLTQMKKDIVKADESGDKEKSARLLEIYMKAKDYE
                     KYRTDCQKRATRMLKKHRETRKEILTEYILESNRTVNNLERTLKLYQELMQYDTFLAQ
                     AVGDDSSNVIKDELNYLSLLSANKEFKANINFDIESQTLKDTINEVEGKLVYQVKKEL
                     SLQEKELLDVITDLQNFLYDRKAECFESRKSVEKERLLIDRMQEAMNCHLEHMHNMAS
                     LNRRWDSEVLEILVHDIRQGETHDIKVVDAAAKIRLALKEYDIKALHFKTMYENELSY
                     LVMQSSRVSEENEIHNDFVVTTLENQMRFTKEQIDLANSEYEMRVDAIIRAVNEERNY
                     VEQIYANQAERYQKKLKIMDDAYQANLYHNNHLLTETEDQKIHKILGKELDKIKRQYL
                     DEKNSVLVAMENDVVMANAKKRLRELDDKLEDAMDDAKILRDNTITEMSEQYAYAKDR
                     IEAIKPYLDNKVNILDPTFYGSMQSINQRYRHNLKNAESELDTATKQLIADYVVVYNS
                     MRPEPSNPELRQKMSELVGTRERLNQEYSLKMQSIENDFNKSSQEYDNQIRLFREKVD
                     ADKALLLNKHQQVKQRISNDLAQIDIGYLGFQKKQITQHENSIRHLTEEYQEAKNASQ
                     VLRENLSLEFGHLLSSYKPYIRLLKKDRDIRDVFSEVNRKARKKLHSEQKEIKKRMRI
                     TKLIP"
     gene            complement(10482..11660)
                     /locus_tag="PLP02_02945"
     CDS             complement(10482..11660)
                     /locus_tag="PLP02_02945"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HPF42442.1"
                     /translation="MKYKRIFLIVMDSVGVGAMPDAADYGDEGANTIGNLAKATGGIR
                     LPLLESFGYGNLTDIKGVHPVINPLANTLKMAELSSSKDTMTGHWEMMGIRTTTPFKT
                     FTDTGFPPELIEELEKISGRKIIGNVAASGTEIIKELGPRQMETGELIVYTSADSVLQ
                     IAAHEDIIPLEELYSICRKTREIMMKPEWRVGRIIARPYIGNPQTGFTRTPNRHDFAL
                     SPTGDTVLNFLERAKYDVISVGKIRDIFNGSGITRHQGIVSNHDGMEKTMAIANDDFT
                     GLCFTNLVDFDAKYGHRRDPFGYKNAMEEFDADLKVMLEKLNADDLLMITADHGNDPT
                     FKGTDHTREYIPLFIYNRDLNGSFLPTSDTFADIGATVADNFEVEQTSYGKSLLKELK
                     "
     gene            complement(11666..12571)
                     /gene="xerD"
                     /locus_tag="PLP02_02950"
     CDS             complement(11666..12571)
                     /gene="xerD"
                     /locus_tag="PLP02_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013173126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPF42443.1"
                     /translation="MLKNLEKEYIYYLRVTKGLAPNTILSYSNDVSDYLTFLETNYQL
                     KTPRAITKEHIQNYLARLKRKGMDPKSVTRKLSALRSFHQYLMQEREVDENLVVKIPK
                     PKTTKSLPVVLNTDEIGRLLEAARGKGSSLDYRNVAIIELAYGSGLRVSELTDLEISD
                     LHLNMALVNITGKGNKERIVPLGGEAIVALRNYLGNARQKMNPKNREAVFVNRQGEKL
                     SRVGLFKLIRSLALKAGIDKPISPHTLRHTFATHLLENGADLRSVQELLGHEDILTTE
                     TYTHISRRHMTEEYEKAHPRARKEE"
     gene            complement(12573..13694)
                     /gene="hemW"
                     /locus_tag="PLP02_02955"
     CDS             complement(12573..13694)
                     /gene="hemW"
                     /locus_tag="PLP02_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPF42444.1"
                     /translation="MIKGLYVHIPFCRQICHYCDFPKMISDRSHREKYVNALACEMQM
                     KKTLLENVETVYLGGGTPGYLEEDLMLIVLESIRDVTHKNVFKEFTLETNLIDITPEN
                     ARLWKQGGINRISVGVETFSLDLLKSIGRTGGYQMAKSALETLRRAGFDNINFDMIYG
                     LPGETTELVEKDIASIGKLRPDHVSWYSLILEKKTRLYHDVMAGIVKLPEDDLVYDMS
                     DMVIDGLENLGYNRYEISNFSHPGKESLHNTSCWNLEEYLGVGLGACSQYDSKRFRNI
                     SGWKGYIEAVLDGKGILTTEEMFEFELERVMLGLRQVNGIDKNEFKHETNLDVFERFP
                     ALKKHLETGLLTDVSGRIRLTRKGLDLANTVMRDLFGEE"
     gene            complement(13802..15631)
                     /gene="lepA"
                     /locus_tag="PLP02_02960"
     CDS             complement(13802..15631)
                     /gene="lepA"
                     /locus_tag="PLP02_02960"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPF42445.1"
                     /translation="MEDYQKMRERQKKIRNFSIIAHIDHGKSTLADRILELTNAVPMR
                     EMKSQLLDSMDLERERGITIKLNSVELRYKAKNNEEYIFHLIDTPGHVDFTYEVSRSL
                     AACEGALLVVDATQGIEAQTLANVYLALDNDLEIIPVINKIDLPSARPAEVRKEISDV
                     IGLDTDGAIDASAKEGIGVKEILEAIVEKIPAPKGDPELPLQALIFDSHYDTYRGVIP
                     LLRVENGVIRRQDKIRLMATGGVYEVTDLGVYTPKEESRDFLGAGDVGWIAATIKNVE
                     KVHVGDTITNNDKPAKNMLPGYRKLTPMVYSGVYPVDADDYLDLKDSLEKLILSDSSL
                     IYEPESSEALGFGFRIGFLGLLHMEIFQERIEREYLQNVIITAPSVNYRVNLTDNTTI
                     EIDNPAKMPAVQRIRSIDEPYVKATIIAPSDYVGNIMELCQNKRGIFKDMQYVSAIRV
                     ELIYEMPLSEIIFDFFNRLKSGTKGYASLDYELAGYRESKLVKVDILLNGEPIDALAL
                     IVHQDFAYPRSLRICQKLKEILPRQLFEIPIQAAIGSKVIARTNIKALRKDVLAKCYG
                     GDVSRKKKLLEKQKEGKKRMKAVGSVEIPQEAFMAVLSLDDEK"
     gene            complement(15751..16082)
                     /gene="rnpB"
                     /locus_tag="PLP02_02965"
     ncRNA           complement(15751..16082)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLP02_02965"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(16100..18235)
                     /gene="pnp"
                     /locus_tag="PLP02_02970"
     CDS             complement(16100..18235)
                     /gene="pnp"
                     /locus_tag="PLP02_02970"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPF42446.1"
                     /translation="MSENQTFTREFCGKTITVETGRYAKQATGAVMIRMGDTAVLSAA
                     VVGKEPSRQDFFPLMVLYQERLYAAGKIPGGFLRREGRPTEKEILTSRAIDRPLRPLF
                     DDYFRNEVQIVDTVMSVDPDNSPFVAALLGSSLALGIGGVPFNGPVAGVVVAMIDGKF
                     ICNPTAEELVVSDLDLTVAGTKYAVNMVESGSKELSEDVMLEAIMFAHDKIKELVAFE
                     EEIITSVGKEKMEIELSTVPDDIDKEVRKLEKGRIIKAITIPEKLARRDAIGTIEEEI
                     RETLTGEYKSQGFDKDEIEEKIKFADRVLETIQVDEVRRLITDEKIRPDGRKLDEIRP
                     LSAEIDLFERTHGSSMFTRGQTQALATVTLGALGEAQIIDGVSVEDGKRFMLHYNFPQ
                     FSVGETGRYGSPGRREIGHGALGERALLQVLPSEEEFPYAIRVVSEILESNGSSSQAT
                     ICAGSLALMAAGVPMKSAVAGIAMGLVKKGDNYTILTDIQGLEDHFGDMDFKVSGTRN
                     GICSLQMDIKIDGITREIMKEALAQAKKARMEILDVMDATIAKPKAELSKYAPKVRMM
                     MVPVDKIREIIGGGGKTINEIIEKCNQVTIDIEQDGRVTIMHQDMFFIDMAVKMIEDI
                     IREVEVGEVYQGTVVRIEKFGCFVELWPGQDGLVHISKLAKERVEKVEDIVKLGDVIT
                     VKVIGIDDKGRVDLSRKAVLDSIGNKEQK"
     gene            complement(18361..18630)
                     /gene="rpsO"
                     /locus_tag="PLP02_02975"
     CDS             complement(18361..18630)
                     /gene="rpsO"
                     /locus_tag="PLP02_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPF42447.1"
                     /translation="MALTKERVHEIVVEYGLKEGDTGSPEIQVALLTEEINSLNEHLQ
                     VHKHDFHSRRGLLMKVGKRRSLLKYLQSVSMERYQALIKKLGLRR"
     gene            complement(18754..20517)
                     /locus_tag="PLP02_02980"
     CDS             complement(18754..20517)
                     /locus_tag="PLP02_02980"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HPF42448.1"
                     /translation="MRITSTKLMNYIRCYRYAALNYHQDRDDDQEEKTVLPVDFGQIP
                     VENGDYESKRLDNPDTAKITRILREKAREDILASLAAAIGYPDKERNKPEARIEIPFE
                     ERFTLYAWAARMIETENENTYYLISAATSHNLTEICDIDGRRRRPLFTKIKDQTYKPN
                     RQLITEAGFSMAKYMARNLESGRLLYDALFRAYVFDKSDSNKKTETVLVLLNSEYVRN
                     KLEPEPEMLSFFKISELLPAAREILETDLYRMINHIKLNDDSRCLLVKNECLPHTAFA
                     CPYVDFCFEHVPKKNSIFSYFHQHLGFREGKKRTDPVHETYELVNSGMVDMLDVPISW
                     LQRECNLMQRYCVQNDYEFINRKKISDKLESLVYPLYFLDFEAYPSPIPRFTGEKCYT
                     QSVFQFSVHVQKDSKTDKSGLIHYDYVMPSDRDNRRKLVERLLEVIPDGNSSVIVYNQ
                     TFEDNRLQELAQLFPVYKERLDNIRDRLFDLLKVLKNDSPFYLAKGYSKAEAKAYNYY
                     HPDLSGSYSLKSVLPVFAEDAYKGMAIANGLEAYLQYAYLPYLSMENRMQIREDLILY
                     CNQDTMSLFVILKGIISRLAE"
     gene            complement(20588..21013)
                     /locus_tag="PLP02_02985"
     CDS             complement(20588..21013)
                     /locus_tag="PLP02_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42449.1"
                     /translation="MEIEIYTDNLTKKDVRNNRKKTYLHLIMTDGDSVLAFKRQGDGL
                     LYFPFAETEDETFADAIKKSGARLSLILTRHDEGQSTISYYYVLEGKTPLFFPLPRNI
                     EAIWIRKDNMMMLLDDYAGEDPEGAKTEEREFIAMMNII"
     gene            complement(20925..21656)
                     /locus_tag="PLP02_02990"
     CDS             complement(20925..21656)
                     /locus_tag="PLP02_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42450.1"
                     /translation="MRGKFTIKNTIIYILGLTFLGINVAFIQKTNLGMSSWDALNRNF
                     YEGIPIEYKYLTPIFALVIISIAYLIQLKKPDLWMLFPVLISFYIGFVIDLLLLVIPS
                     VVDENLFWNLMYLFIAIIICAVGINFLLYSKYPLPALDELCYGMHKLFRVSFGKAKII
                     GEFIALILTIFAGLYFHHQSEWFYIGPTTVIYGLGIGFVIDWLKKPVGHFLEALDGNR
                     NIHRQPDKEGCKEQSEENLSTPDND"
     gene            complement(21769..24090)
                     /gene="lon"
                     /locus_tag="PLP02_02995"
     CDS             complement(21769..24090)
                     /gene="lon"
                     /locus_tag="PLP02_02995"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019391303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPF42451.1"
                     /translation="MYEANKLYSDRLPVVPVRGVIFLPGSDVRVEVGRDFSKNAIYEA
                     ENNRDGHVLLVFQSNPEILEPEFTDFLGVGLLARISVKIKLPNGNFKIKFEPIGRVEL
                     HDLIAKEPFYLIDFTTMVLQPENLDEEQVLLKMVSKETVENASFLFRDSKKVLDSMQG
                     GITSDKLCDLIASELRISENERAKYLLTNSINKRLQYILEDIEKEKNLQTIESKINES
                     VRRSADQNQKEYYLREKMRAIQEELGDSKQDDASKLLEQINASDMPDRVREKALKELK
                     RYEYLSANSSESNVVRTYLETLISIPWQTTTEDEKDINVAIQKLESSHYGLEKVKERI
                     IEYLSVKMLTGKNPQTILCLSGPPGVGKTSLAKSIANALNREFVKTSLGGVHDEAEIR
                     GHRRTYVGALPGRIINGMIRAKVANPVFLIDEIDKLVINQNNDPSSAMLEVLDPEQNQ
                     FYSDHYVEEDYDLSQVLFITTANYLENVPAPLRDRMEIIELSSYTEIEKFHIARRFLI
                     KKEIENHGLEEGKLEIEDETIRQIIKDYTREAGVRQLERLIGSITRKAIKEILVDKLE
                     KVTVGPDDLYHYLGKPLFFNNGVQKEDMVGVVTGLAYTIAGGDTLDVEATYYEGKNNL
                     VLTGNLGNVMKESAMAALSYVRSNSEKLGIDPKVFDNTDIHIHVPEGAIPKDGPSAGV
                     TIATALISAFGKKKVNRFVGMTGEITLKGRVLPIGGLKEKAIAASRSGLKKIVIPKGN
                     EKDVEDIPSEVRDVLEIDFAETLEDVLKIALVA"
     gene            complement(24258..25553)
                     /gene="tig"
                     /locus_tag="PLP02_03000"
     CDS             complement(24258..25553)
                     /gene="tig"
                     /locus_tag="PLP02_03000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPF42452.1"
                     /translation="MMIDKLSGSRVHFTITVTGEMFEHALDHAFEVVNEKTEIKGFRK
                     GKAPRSVYEAHYGIESLYEEAVEHAIGDTYYDAITENDIDVVAQPKIDFDFKNVKRGS
                     DFTYEVTVAVRPEVVLGEYLGTEVKLLPDVASDVEIDTEITREREKNAEMVLKEEGVL
                     EEGDTAVFDFEGSVDGVKFDGGSAENYELVIGSNQFIPGFEEQMKGMAPETEKNVIVS
                     FPEDYHEKSLAGKESVFKVTLHEIKNRVVPQLDDEFVKDLNLDNISTIDEYKAYVKNQ
                     IETDKKKQNENQIRATVIEKATENATFDLPEEMIEEEVKRADEDNQNRMKQYRLDFDT
                     YLKYMGKTKEEYLTELKEEATKTLRQQMVINAIAKKENLEADDKETEAKYQEIADQYK
                     SQNITLDQVKNAIPVSAIKEEISFKKAIDLLIEKANVIR"
     gene            complement(25631..26545)
                     /locus_tag="PLP02_03005"
     CDS             complement(25631..26545)
                     /locus_tag="PLP02_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HPF42453.1"
                     /translation="MTKFKKAFWITIGIAALFFAILIIISSVLNIGDKLNGIHPYVAY
                     AFYGISVILFYILIVRPVFLIIFSPTFSMDSLFTDEENARKNYHMYRKVSDNLLKEEY
                     LAKEEKESLQASLSDPVKLKQSLSFVFDQKIKKELNKIIIRHAESVFLSTAISQNGRL
                     DSIAVFTINLKLIKELVLKCGFRPSYASLGRLSFNVLTAAVVAESLEDIDFNELFPSK
                     GLSALSEVPFLKTIIGSFAQGVGNALLSLRVGIICRNYLFMNLKGVDRNKIRKLAFAE
                     AVTLLPGVLAESVKKLPSRFKSIFEKVF"
     gene            complement(26542..27444)
                     /locus_tag="PLP02_03010"
     CDS             complement(26542..27444)
                     /locus_tag="PLP02_03010"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPF42454.1"
                     /translation="MKKIILDSTARLEIPNLTLAAGFFDGMHLAHQKLLETARDKGHP
                     NGMLTLSEHIPSLLGNRKYQMLTTLSDKEEIASMYGLDYFIVLEVDRALIELTPEDFA
                     FRYLSSCKTIIVGFDFSFGRHGSGKPQLLEKLFPGKVEVVPEMQSGGEKIGSTRIKQY
                     LESGDIEKANNLLGRPYRIKGTIVKGHRRGHVLGYPTANVDYKDYILPLPGVYMTKTT
                     IENESFFSLTNIGDNPTFSDRQMTLESHLFGFGRESYNETAMIDFLAYLRPEIRFDTP
                     EELKKQVEADEMKLKKAIENGRIK"
     gene            complement(27441..28334)
                     /gene="truB"
                     /locus_tag="PLP02_03015"
     CDS             complement(27441..28334)
                     /gene="truB"
                     /locus_tag="PLP02_03015"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPF42455.1"
                     /translation="MDYLGNQFGLLLVEKPAGLTSHDVVNQARKVLKTKTIGHTGTLD
                     PFATGLLVLAIGRATKLMPYLNNHDKTYEATMILGLETDTLDPSGKVVREENCENIRK
                     EAIFKALEHYTGTFYQEAPAYSAVKVAGRKLYEYQRKGLKIPAMPQREVSVYSYENID
                     VKDQKAGKIKVSFTVKVSKGTYIRQLAKDLGAYLNIPASLVALKRITSGPYSVTDAIP
                     YEKLMEGNLSIIDPLAGMGFSKIVMSDDITKKIDNGAFVEREYFPDESDTIIYDKYGK
                     PVAIYRFDPERKVMRMAVKLL"
     gene            complement(28337..28642)
                     /locus_tag="PLP02_03020"
     CDS             complement(28337..28642)
                     /locus_tag="PLP02_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPF42456.1"
                     /translation="MKMPLNEHELKLDTGRYVILFVLKWDAACQQQEIWLSELESEMH
                     FNGITIDTEQMPMVTRSFRAEAVPLVVFIQEGIELQRIEGFHSKDALRSWLLSSEVF"
     gene            complement(28659..>29753)
                     /locus_tag="PLP02_03025"
     CDS             complement(28659..>29753)
                     /locus_tag="PLP02_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPF42457.1"
                     /translation="FDELGAGTDPKEGASLAISLLDYLRQVGCHAMVTTHYPELKVYA
                     YNLDDVVNASVEFDIDSLKPTYRLKIGIPGTSNALEIAKRLGLEESIIEKAKTVSLTF
                     DNETTNLIKKLEKESRDLETEIAKTREMQANIASLEESLKTEIMQSRSKQNKIISELK
                     EQKHAELEKAKEQAMELIRELDGMKKNANVKEHELANMKYKVRNMDTVEEKYQKVDQS
                     KIKIGDLVNVIPYQRIGVVTKQRNAKEFEVQLGVLSAVFSESDLEYQKKADQSEPRSE
                     VRVSKEGIIHAELDLRGKRYEEAMEELDKYVDDCLFHHLEFASIIHGYGTGALKKGVE
                     EYIKSHNTIKKSRSGGQNEGGHGVTIIYFK"
BASE COUNT         7659 a         7055 c         6691 g         8348 t
ORIGIN      
        1 tcatcaaaac tcatgatgat atcagcgcca aatacatttt gagcgcggat gctgtcttcc
       61 ggagacatgt gaagaagttc gccggacaaa tggtggcgga atgtcactcc ttcttcttga
      121 atcttgcgaa tcttggaaag agaaaatacc tgatagccac cggaatcggt cagaagcgct
      181 ccgtcccaat tcatgaattt tttgacatct ccatgcttgg caatgatttt ttctcccggt
      241 tcgagccaaa ggtggtaggt attggctaaa ataagcccct cgcttatctc acgcgcttct
      301 gtggaatcga gtgtctttac agtcgcctgg gttcctaccg gcatgaagac cggggtaggt
      361 atgtcttttg attcggtatg taaaatccca catctggcgc ctgtttgttt gcagatgtgg
      421 gtaatttcgt gactgaacgg catgttttta gagaatgatt ccgaaaacct cgccggagat
      481 gccggcggcg tttccttcgt cggtgtcaat atgcattgct ggcgagaaat cggctcttac
      541 gcggataacc acatctccaa agacaagcga gcgttcggga gtgtcaacac gtacagacac
      601 gatgtccttg tcagcgacct gatataggct ggcaacttca ggagtgagat ggacatgtct
      661 tttcgcgaca atgacgcctt cctttatatc aatgaaccct ttcgggccca cgatccggca
      721 cggcgcggtt ccggcgatat ctcccgattc gcgtacggga gcagttatgc caagggttct
      781 ggcatcagtc aaggatattt ctacttgggt cttgcttctg gttggcccga gaatcgtcag
      841 attcatttct tttttcggtc cgatgaccgt cactttttct tcacaagcga actgtcccgg
      901 ctgcgacaac atcttttttg ctgtaagttt gtatcctgat ccgaaaagag tttccaaatc
      961 cttttcgttt atgtgcacat gtctggcgga tacctctaca agaatttttt tatccataat
     1021 gtcctcctgg tgctttctca tgattttact aggtttattt tactaccaat cgcctatttt
     1081 cgcaattcaa attgttttat tattgtcttt tgagacaata tccggtataa ttgtttgggt
     1141 cttgggattg tttcccggaa aatggagtga ccttgatgaa cgagttactg tggttcgctt
     1201 ttgccttggc gaatttcata ctttttttgg gaatgtacaa actgttcggt aaaaccggca
     1261 tttttgtttg ggttgccata agtacgatca ttgccaacat ccaggtcacg aagaatatca
     1321 ctctattcgg tgtcgaagcc acgctaggaa acattaccta tgggacgata tttctggcca
     1381 ccgacgccct aaacgaaatc tatgggaaga aatccgcgca aaaagcggtg ttgatgggat
     1441 tctatgtcat ggtcgccaca gtcgttttga tgcagatggc gatctggttc attcccagta
     1501 catctgattt gaacaatgat gcgcttcggt cgattttcgg tttctttcca agacttctgg
     1561 gagcttcgct cacggcattt ctcataagtc agtttctgga tgtccatctt tttcagaaaa
     1621 tccgcgagaa attgccatcc gacaaatttc tttgggtcag aaacaacggc tccactttca
     1681 tcagtcaatt ggtcgatacg gccatattcg ttccaatcgc ttttttggga gtatatagtg
     1741 gcagcgtgct tttacagatc gggttgacta catatcttat aaaagtcatc gtcgcgatgc
     1801 tcgacactcc gtttctttat atatgcaaaa gaatcatccc gtttgataac aactgaaaga
     1861 aaaaagccgt gaaggctttt tttatcggat gaacatggaa tccccaaagg aaaagaaacg
     1921 atactctctt tcgatagcct ctctgtaggc attgaaaatg atctcgcggg acgagaatgc
     1981 cgaaaccagc atcatcaagg tcgactttgg cagatgaaaa ttggttatca gtccgtcgat
     2041 cgccctgaac tggaatcccg ggtagatgaa gatgtcggta tatccggagt ccggacgaaa
     2101 ctctccatat ttggcatata cgctttctaa ggtcctggtc gaagtcgtac cgacagcgat
     2161 gattcttctt ttctccgttt tcgctttatt tagtgctttt gcggcttcgg tatcaaacac
     2221 atagtactcg ctatgcatct tgtgtttggt aaagtcctct gtttcgaccg gacggaatgt
     2281 ccccaagccg acatgtaggg ttaaaaacac ggtttctatt cctttttccc tgatttcatc
     2341 aagtagggct tttgtgaaat gcagtccggc tgttggggcg gctgcgctgc caagttcccg
     2401 tgagtagacc gtctggtaac ggtttttatc ttccagtttt tcatggatgt aaggtggcag
     2461 gggtgtttct cccagtttat cgagaatctc cgggaatacg cctttgtaat acatccgcat
     2521 ttttctgatg ccgtcatctt tttcttcaat acattttgcc atcaaaagtc cctgaccaaa
     2581 cgagacaatc gattcttttt ttattcttct ggccggtttg gccaaggttt cccaaacatc
     2641 gtcttccatt tgtctcaata acagtatttc gatatgagct ccggtgtcag gcttctctcc
     2701 gatcagcctc gccggcatca ctttggtatc attcaaaacg agaacatcgc ctttttccag
     2761 gtattgcggt aaatcccgga aatgtctgtg ttcgagattg aaattagtcc tgtttaccat
     2821 taaaagcctt gagtccgtgc gatttggcaa cggagactgg gcaatgagat ttttgggcaa
     2881 tatgtaatca aaatcgcttg ttttcattca aaaaagactc tcctggtaca tttttttcag
     2941 atgatgatac gctttttctg tcgccacccg tcctctcgga gtccgggaaa gatatccgct
     3001 ttggataaga taaggctcgt acacatcttc caaagtctgc ggatcttcgc ttatcgaagt
     3061 ggcgatcgtg tcgagaccga ccggaccgcc tttgtatttt tcgatgatgg tcgaaaggta
     3121 ttcccgatcc ttccggtcga gaccgcattc gtcaattttc agcttctcca atgcttttct
     3181 ggtgatatct aaggtgatcg tgccgtcacc gaggatgtcg gcaaaatctc tcactctccg
     3241 gaacaaccgg tttacgatcc gcggagttcc gcggctgcgt ttggccagtt cgatgactgc
     3301 ctggctatca atcggacagt catagatccg ggaagtcctt tgacaaatgg tctccagctc
     3361 gccgtcagta tagaaatcaa ggcggtgcac aatgccaaaa cgttcgcgta gcggcccaga
     3421 aagatcaccg aaacgcgtgg tggctcccac cagggtgaaa ggcgggagat caactcggat
     3481 cgacttggct ccggaatcct tccctacaac aatgtcgatg acaaaatcct ccatcgagga
     3541 atacaatatc tcttcaacaa tttttggcat ccggtgaatt tcatcgataa acaaaacatc
     3601 gcccggttca agcgaggtta gaatggccgc cagatcaccg gttctttcga tcgtcgggcc
     3661 actgcaggtc ttcatctgaa ccccaagttc attggcgatt acctgggcca aggtggtttt
     3721 cccgagtccg ggagggccgt aaagcaaaac atgatccaac gactcttccc gcttcctcgc
     3781 ggctttgacg gctatttcca tcatgtcctt gacattggtt tgtccgatgt attccgaaaa
     3841 catctgcggg cgcagagttt cttcgatttc atcgtccatc aaaaggttgt tgctgatgat
     3901 tcgtttatcc ataatgcaca cctcatattc tattatagca aatcaccgaa taaattgtgc
     3961 attattttcg ctcatttctt gaattatgac tttatctata ctataatcat aattaagaag
     4021 gagtcacata tggtaaaatg gcaaaacatc gcgatatcat agcaacttac cttcacgatt
     4081 ttcccgaaat caaaaaggct ttccaaagcg ctcaggaaca acttgagcgc cgtttgtcca
     4141 tggtaaagga agacaacgcc cacgaacttg ccgatatcga gaattcatac aagacatacg
     4201 aagacgacca tctccacaaa tatatcgata aaaacgatga attcaaagaa aacatcgact
     4261 tcataaacgc cgaataccgg gagcttctcg acgatgtcga aagcgatatc tccctcaaca
     4321 acaaaaaaac cgaggaactg atcgaagccg aaaccaagga attcgaaaac atcctcaacg
     4381 atatcgatag cttaaagcaa gaagccggcg accaatataa cgaaatgaca agaatgatcg
     4441 gcgaaaagat cgatcacgaa atgcatgtcc atcacgaatt catcgcccag gaaaatgcga
     4501 aattcctttc tacccaggaa cattaccagg aaattaattc tgaccaggcc aaccgtttga
     4561 tgtgggcaat cgagcaatcg aaaaacgctc ttgccgatct gagcagccgt ttagaggaaa
     4621 aatctagcca gcatgccgca ttcatggatg aatctgccac tgaaatattg cattctttga
     4681 gagaaaccaa agatcgtgtg aattctcttt tcaaaggaac caccgatctc ttttccgaac
     4741 aaaagcatca tatcgagcaa ttgaatcacg aacgccagaa accgcattcc cagctcaatc
     4801 agaccatcgt ccgccaatac gtcaagaaaa tccgggaagt gaaccaaaaa aagatcgctt
     4861 tcgaccagat gatccggaaa gaacttgatt cttctctcga agtaatcgga gagagaatca
     4921 tcaaattcga tgctgagaaa aaccggttgg aaactgaaaa agcgattctt caatatgaga
     4981 ttgtcaagaa aaaagccgat tatctgctaa accgcaacaa actgatcgcc gacagcctta
     5041 tctccaaata tcaaaatgaa attaaaaaaa tcaagatcga ctctttcaga cgtgtcgagg
     5101 aaatcaagct cgcgtattac atgccggcgg cttttttcca gaacagcatt aatctctaca
     5161 gcaatttcgc cttttacgtt aacgaatcct tcaccgatct cgacatgttg ctttccgatc
     5221 ttaccatgac aaacaaacga attaccgatc tggaaacaaa ctacgtgacg catagcgcca
     5281 aagccgtcga ggattacaag cttcgcgtga tggcccagat aaacgaggta accggaaaac
     5341 tgacaggact gattaccaaa atcgatactc tcagccgcga aatcgttact ctcgaatcaa
     5401 gaaatcaact ggaaatcgct gaaatcagga aaaaaatgga atctaccgat atcatgggcg
     5461 attaccagaa acatttggcc aaattagaaa acgacgaata tttcgcctgt tatcagcatg
     5521 acataaactt aaacaagatc aaggcagcca accgttgcga cggcgagttg ctcgggatcg
     5581 aacaatcggt tatcaaacta catcgcgacc gggaaatgat tgatgtcaga gaaagacaag
     5641 caaaggcgat gtcgcaaaac gagaaagaaa tccgcgatct tgcttatgac aaggctcttg
     5701 ccattttccg ggctgcctat gatagaaaca aactggcatc ggacattcag aaccaaattg
     5761 aatcggaaaa agtcgaattc tcttttaaag cccacaatta cctcttcgcc cgcaaatatg
     5821 aagaacttag aaacaaacat cacaggagcg aagcagacgg cagcgaaggt gtcgtggaat
     5881 atgtccacaa cgcccaaaaa atgattgatg ccaacaacaa cgaactgaaa gacttcatcg
     5941 ccggcatcgc gcaaggacgc aacgcacgcg attacgccta ctatctggaa accgtcaaga
     6001 caaacatgat catggctctc aaagccgaac tgaaaacaaa aaccgagaaa aaccgcaaag
     6061 cgattgatct atataaggaa catttccaaa gagcgcatga gctgttgcaa ggcgcttttg
     6121 cggatatttc gcatcagatc aagcagcatc tggctaatct agacgaaaac atggctttgg
     6181 tccaggccaa tatgatcatc cgcgatcacg cttatctcca tcaaatagcc gatatcttcg
     6241 ccaatgccta tgacgatgct ttttccttca tcaaggggat ttcatccaaa gcagatccgt
     6301 cattgcttga aaagcaaaaa gaaagcgcgt taaacaatta cgtcatctta gcggatatca
     6361 cattgacagc tttacaaaag ccaaagattt ccgccaaaaa aaccaattcg gtattggaac
     6421 actattatgt aaccacaatc gatttgataa atgattccat caatacggta accgcatttt
     6481 acgcagacat ggaaattttc gtaaagaaaa acgacatttc attcatcaaa aaagcggaag
     6541 aaaacgaagc cacaagcgaa aagatgatca ctgaagtata cgatgagttg atcgtcaagg
     6601 ccgcccgaat gggaaacgac agcgacaaaa aagtcaattc gatgaaaaag atcaccactt
     6661 ccatcgaatc cgaattgaaa agaaaagtcg ccaaggtgaa ttcgctttac ataaacaaca
     6721 tgaaaaccga agccgacaaa ctcggttaca taagcaagtc gttcgaaaag atcatgtccg
     6781 acaacgaaag agctttggca acttaccgca tctcggaaac agctcggatc aaacgcgaag
     6841 gtgccgacag ggaaaaagct tacggaaaat tcattcgtga ctacgaaagg caaaaagccg
     6901 ccagcgagga aacttgccgg ttggaaggcg attattcggc cctttctgcc cgcaagggtg
     6961 tgtctgacat cgaatcaatg ttaaaggaat acgaggaaaa aacaaaatta ttgccgaaag
     7021 aacgcgataa atccatcata gccctggaat ccggaaaaga aaccaagatt atgaaacaga
     7081 ataccgcact catgcaacaa ctggcaatca tcgagggtaa caaattcagt tcacgtccaa
     7141 aatttatgaa agaaatcgaa gcaatcaaag accggctgcc gactgattat ctacatctgt
     7201 accaggaaat ccaaaaagcg gaatccgact tattgcaaca atatatcgag accgaaacct
     7261 cgatcagccg cgattttcaa ctgttccttt cgggacagga aagccacaag cgacttttgt
     7321 ccgacgaaaa actagtctat tccccgtttg aaggtttcct agatttacag gacaatctga
     7381 tcgaaaaagc cgccggcgct tacgaaacag cgattgacaa gtcagatctg gccacgaaaa
     7441 agattaagga aaccgaaaac aattccaagg aaaaacaaga ccggatcatc aacgaataaa
     7501 ttaaaaggag gtgtccttga tggctgaaga aaagaaccag ccgaaaactg gcaataccaa
     7561 cagcgaagct tatctagaaa aatcacgcca gtcctacgaa aaggaaatgg ctcttttcga
     7621 acagttctac aagaatatta gccaccgagc cgacacccac cgtcagatca ccgttaacaa
     7681 tgtcaacgaa gtcgaaaaaa ggcttacctc tctcaaagag gaagccgaca agttacgtga
     7741 ttcgatcttc tatcatgacg aaacggtcat agtcgaccgt caggagatca tcgccaagac
     7801 cgaagacgat gtccatcaga acaactcgct tcttctggcc aacgatcgtt acgacgctga
     7861 aaatctggtg tcaaccgtcg attacatgaa caaagcgctc attcaggtca aactggattt
     7921 cttcgatgcc tataacaaca gttatctgaa atcaatcgtc gccaacgacg atttcaccgc
     7981 cctgttcgaa gccaagtcgg aaactttcca aaaagttctc gacaaacatc aaaaaaacgt
     8041 tatgtcgatt tttcttgatc ttaacgagga aatccgcgaa atggacgata agatcgccag
     8101 aatcatgcac gagaaaaacg atacgatcag ttccatcgtc cagttctacg atcaggaaag
     8161 caaaaactac gtggacaacc agttgatgtt ctcaccggcg tcggatccga catcgatcga
     8221 cattcaagct ctggtatcag acaaaatcaa ccaatttcat accttcagac gtcatttgga
     8281 agaacagacc gctaatgtcg aaaaacatat cgtcggcaat ttcacggacc ttgaccacca
     8341 aatcgtcggg aaaatggaga aacatatcgg aaacactatg ttgaacaatt ccgatttctt
     8401 caacagcccg gaagattacc tgacccagat gaaaaaagat atcgtcaaag ccgatgaatc
     8461 cggcgacaag gaaaaatccg ccagactgct agaaatttac atgaaggcga aagattacga
     8521 aaaatatcgg accgattgcc agaaaagggc aacaaggatg ctgaaaaaac accgggaaac
     8581 ccgcaaggaa atccttaccg aatacatact cgaatcaaac agaaccgtga ataatctcga
     8641 acggactctg aaactttacc aggaattgat gcaatacgac actttcctgg cccaagccgt
     8701 cggagacgat tcatccaatg tgatcaagga cgaacttaac tatctgtcct tgctttccgc
     8761 caacaaggaa ttcaaggcca atatcaattt cgatatcgaa agccagacat tgaaagacac
     8821 aatcaacgaa gtcgaaggca agctcgtcta tcaggtgaaa aaggaattat cgctccagga
     8881 aaaggaactt ctcgatgtca taaccgatct ccagaatttc ctttacgacc gtaaagcgga
     8941 atgcttcgaa tctcggaaat cagtcgaaaa ggaaagactt ctgatcgacc gaatgcaaga
     9001 agccatgaac tgtcatcttg agcatatgca caacatggct agtctcaatc gtcgctggga
     9061 ttcggaagtg ctggaaatcc tcgtccacga catcagacag ggcgaaaccc acgacatcaa
     9121 ggttgtcgat gctgccgcca agatcagatt ggcactaaaa gaatatgaca tcaaggcatt
     9181 gcatttcaaa actatgtatg aaaacgaact cagttattta gtcatgcagt cttccagggt
     9241 cagcgaagaa aacgaaatcc acaacgattt tgtcgtcacc actctggaaa accagatgcg
     9301 tttcacaaaa gaacaaattg atctggcgaa ctccgaatac gaaatgcggg tcgatgcgat
     9361 cataagagcc gtcaatgagg aacgcaatta tgtcgaacag atctacgcta accaggcgga
     9421 aagatatcag aaaaaactta aaatcatgga tgacgcctat caggccaacc tgtatcacaa
     9481 caaccatctt ctgaccgaaa ccgaagacca gaagattcat aaaatacttg gtaaggaact
     9541 cgataagatc aaacgccagt atcttgacga aaagaattcc gtgcttgtcg ccatggagaa
     9601 tgatgttgta atggcaaacg ccaaaaaaag gcttcgtgaa ctagacgaca aactggaaga
     9661 tgcgatggat gatgccaaaa ttcttcgcga caacaccata accgagatgt cagaacaata
     9721 cgcttatgcc aaggaccgta ttgaggcgat caaaccgtat ttggataaca aagtcaatat
     9781 tcttgacccg acattctacg gttccatgca aagcataaat caacgatacc gccacaacct
     9841 caaaaacgcc gaaagcgaac tggacacggc aacgaaacaa ctgatcgccg attatgtggt
     9901 cgtttacaat tccatgcgtc cggaaccgag caatccggag ctcagacaaa aaatgagcga
     9961 acttgtcggc accagggaaa gattgaacca ggaatattcc ctgaagatgc aatccatcga
    10021 aaacgacttt aataagtcgt ctcaggaata tgacaatcaa atcagattat tccgtgagaa
    10081 agtcgatgca gacaaggctc tacttcttaa caagcaccaa caggtgaaac aacggatttc
    10141 caacgatctc gcacagattg acatcggcta cctcgggttc cagaaaaaac agattaccca
    10201 acatgagaat tccattcgtc atctgacgga ggaataccag gaagcaaaaa acgctagcca
    10261 agtcttgcgg gaaaacctca gtctggaatt cggacatctg ctttcttcct ataagcctta
    10321 cattcggtta ttgaagaaag accgcgatat tcgggatgta ttctcggaag tcaaccgcaa
    10381 ggcaagaaag aaattgcatt cggaacaaaa ggaaataaaa aagcggatgc gtataactaa
    10441 acttattcca taaaaaaacg tcggtgatcc cgacgctttt tttattttaa ttcttttaaa
    10501 agacttttcc cataggatgt ctgttcgacc tcgaaattgt cagctaccgt agcaccgatg
    10561 tcggcaaatg tatcggatgt tggcaaaaac gaaccattca aatcgcggtt gtaaatgaac
    10621 aggggaatat actcccttgt atggtcagtt cctttgaagg tcggatcgtt tccgtgatcg
    10681 gcggttatca tgagaagatc gtcagcattt agtttttcca acatgacttt taaatcggca
    10741 tcaaattcct ccatggcgtt cttgtatcca aacggatcgc ggcgatggcc gtatttggca
    10801 tcgaaatcga caaggttagt aaagcataat ccggtaaaat cgtcgtttgc gatagccatg
    10861 gtcttttcca ttccgtcatg attcgagacg attccctgat gtctggtgat tccggagccg
    10921 ttgaagatat cgcggatttt accgactgat atcacatcgt attttgccct ttccaagaaa
    10981 tttaggactg tgtctcccgt cggactcaaa gcgaaatcgt gacggttcgg ggtacgggtg
    11041 aaaccagttt ggggattccc tatatacggt cttgcgatga ttcttcccac gcgccattcc
    11101 ggtttcatca tgatctccct tgtcttgcgg cagatcgaat acaattcttc aagcggtatt
    11161 atatcttcgt gagcggcaat ttggagtacg gaatcggcgc tggtgtaaac aatcaattca
    11221 ccggtttcca tttgtcttgg tcccagttcc ttgatgatct cggtaccgga tgcagcgacg
    11281 tttccgatga ttttgcggcc ggatatcttt tcgagttctt cgatcaactc cggcggaaac
    11341 ccggtatcgg tgaaagtttt gaaaggcgtt gtcgtccgga ttcccatcat ttcccaatgg
    11401 cccgtcatgg tgtccttact tgaactcaac tcagccattt tcagggtgtt ggcaagaggg
    11461 tttatcaccg gatggactcc tttgatatcg gtcaggtttc cgtatccgaa cgattctaaa
    11521 agcggtaatc tgattcctcc ggtagcttta gcgagattgc cgatcgtatt ggcgccttcg
    11581 tcgccgtaat cggctgcgtc cggcatggcg ccgactccca ccgaatccat aacaatcaaa
    11641 aatatgcgtt tgtatttcat ttgattcact cttctttcct agcgcgcgga tgcgcctttt
    11701 cgtattcttc cgtcatatgc cgacggctga tatgcgtgta tgtttccgtt gtcaggatat
    11761 cctcgtgtcc gagtaactcc tgaaccgaac ggagatcggc tccgttttcc aaaagatggg
    11821 tggcgaacgt gtgccttaaa gtatgcggtg atatcggttt gtcgatgccg gccttgagtg
    11881 cgagactgcg aatcagtttg aataacccta cccggctcag tttttctccc tgacgattga
    11941 caaaaacggc ttcccggttt ttcggattca ttttctgtct agcgtttccg agatagtttc
    12001 gcaaagcgac gatcgcttcg cctcccaagg gaacgatcct ttctttgttt ccttttccgg
    12061 tgatgttgac aagcgccatg ttcagatgaa gatcggatat ttcgagatcc gtcagttcgg
    12121 atacccgaag tcccgatccg taggctagtt cgatgatcgc cacatttcta taatccagtg
    12181 aagagccttt gcctctggcc gcttccagaa gccgaccgat ttcatcggta ttcagcacga
    12241 cgggcaagga tttggtagtt ttcggttttg gaatcttgac gaccagattt tcatccactt
    12301 ccctttcctg catcagatac tgatgaaatg aacgcaaagc cgaaagcttt ctggtgacgc
    12361 ttttaggatc catgcctttt cttttcagtc tggctaaata attctgaatg tgttccttcg
    12421 tgattgcgcg cggcgttttc agttgataat tggtttccaa aaaagtgagg taatccgata
    12481 catcgtttga ataggatagt atcgtgttgg gcgcaaggcc ttttgtgaca cgcaagtaat
    12541 agatatattc cttttccagg ttttttagca tgtcattcct ccccgaacag gtcgcgcatg
    12601 accgtattcg caagatccaa tcccttacgc gtcagtctga ttctgccgga aacgtctgtt
    12661 agaaggcccg tttccagatg cttttttaaa gcgggaaacc gttcgaatac atccagattg
    12721 gtttcgtgtt tgaattcgtt tttgtcgatg ccgttgactt gtcgtaatcc gagcatgact
    12781 ctttcaagtt cgaactcgaa catttcttcc gttgtcaata ttccttttcc atcaagcact
    12841 gcttcgatat atcctttcca tcccgatatg ttgcgaaatc gtttcgagtc gtattgcgaa
    12901 caggctccca gtcccacacc gagatattcc tccagattcc agcacgatgt gttgtggagc
    12961 gattcttttc ccggatggga aaagttgctg atctcgtaac gattatatcc gagattttcc
    13021 aatccgtcta tcaccatgtc cgacatgtca taaaccaaat cgtcttccgg gagtttcaca
    13081 atgccagcca taacatcgtg ataaagtcgg gttttctttt ccaatatcag agaataccag
    13141 gaaacatgat cgggcctcag ttttccgatt gatgcgatat ctttttccac gagttctgtg
    13201 gtttctcccg gcaacccgta gatcatatcg aaattgatgt tgtcaaatcc ggcgcggcgg
    13261 agtgtttcca gagcggattt tgccatttga tatccaccag tacgaccgat agatttcaaa
    13321 agatccaagg aaaatgtctc tactccgaca ctgatgcggt tgatgccgcc ttgtttccag
    13381 agtctggcgt tttccggtgt aatatcaatc aaattggttt ccagggtgaa ttccttaaaa
    13441 acattcttat gagtaacatc ccggatcgac tccaggacta ttagcatcaa atcctcttcc
    13501 agataccccg gggttccgcc gcctaagtag acggtttcga cattttcaag aagtgttttt
    13561 ttcatctgca tttcgcaagc cagagcattt acgtattttt cccggtgact tcgatcggag
    13621 atcatcttgg ggaagtcgca ataatggcat atctgacggc aaaacggaat atggacatac
    13681 aggcctttga tcacattatc accgatatta tgttaacata attcggtcgt tttgtatacc
    13741 gttttgcctt tgtggtataa aaaaccggtc ccggcaagcc gggtccggca tcaatcaatc
    13801 gttatttttc gtcatcaagg gacaagaccg ccatgaatgc ttcctgcggt atctcgaccg
    13861 atccgacggc tttcattctt tttttgcctt ccttttgttt ttctagcaat ttctttttcc
    13921 tggaaacgtc gcccccgtaa catttggcaa gcacgtcttt tctcaatgct ttgatgttgg
    13981 ttctcgctat gactttggaa ccgatcgcgg cctgaatcgg aatttcaaag agttgtcgtg
    14041 gcaaaatctc tttcaacttc tgacagatcc tcaatgaccg cggataggcg aaatcctgat
    14101 ggacgatcaa tgccagggca tcgatcggtt cgccgtttaa aaggatgtcg acttttacaa
    14161 gtttgctttc gcggtaaccg gcaagctcat agtcaagcga agcataaccc ttcgttccgg
    14221 atttaagccg gttgaagaaa tcgaaaatga tttccgacaa cggcatttca tagattagtt
    14281 ccaccctgat agccgaaaca tactgcatat ccttgaagat tcctcgtttg ttctgacaaa
    14341 gttccatgat gttcccgaca taatcgctag gcgcaatgat cgttgcctta acgtaaggtt
    14401 catcgattga gcgaattctt tgtacggccg gcattttggc cggattgtcg atctcgatcg
    14461 tggtattgtc ggtaagattg acccggtaat tcaccgatgg ggccgtaatg attacgtttt
    14521 gcaggtattc cctctcgatc ctttcctgga atatctccat atgcagaagg cctaagaatc
    14581 cgatgcggaa gccaaatccg agggcttccg aactttccgg ttcatagata agggatgaat
    14641 ccgacaagat cagtttttcc agagaatcct tcagatctag ataatcgtcg gcatcgaccg
    14701 gataaacacc ggaataaacc atcggggtca gttttctata tcccggcaac atgtttttcg
    14761 ctggtttgtc attgtttgta atagtgtcgc cgacatggac tttttcgaca tttttgatcg
    14821 ttgcggcgat ccagcctacg tctccggctc cgagaaaatc ccgcgattcc tctttgggag
    14881 tatagacgcc gaggtccgtc acctcgtaaa ctcctcccgt cgccatcaac ctgattttgt
    14941 cctgccggcg gatgacgccg ttttcaaccc tcaaaagagg aatgacgccc cggtaggtgt
    15001 cgtaatgcga atcaaatatc aaagcctgga gcggcaactc cggatcgcct ttgggagccg
    15061 gaattttttc gacgatcgct tctaaaatct ccttgacgcc tattccttct tttgcggaag
    15121 catcgatggc gccatcggtg tcaagaccaa tcacatcgga gatttctttg cgaacctccg
    15181 ccggtctcgc acttggaaga tcgatcttgt tgatgacggg aattatttcc agatcgttgt
    15241 caagcgccaa atagacgttg gcgagcgtct gagcttcgat accttgggtg gcgtctacca
    15301 caagaagcgc tccttcgcag gctgctaagc tccgtgatac ttcgtatgtg aagtcgacat
    15361 gtcccggggt gtcgataagg tggaatatat attcctcgtt gtttttcgct ttgtatctca
    15421 attcgacgga attcagttta atcgtgattc cacgttctct ttccaggtcc atcgaatcaa
    15481 gcaattgact tttcatttcc cgcatcggaa cggcattggt caactccagg atgcggtcgg
    15541 caagggtgga tttgccgtgg tcgatatgag cgatgatcga gaaattacgg atttttttct
    15601 ggcgttcgcg cattttttgg taatcttcca tggcttcacc attcctttct tttaataatt
    15661 ttacacccaa ttgcccatgt ttggcaatcg ggctctttca tgccagtaaa aaaagcatat
    15721 gtttccaata tgcttttttt gtatcagacc atctgtaagc catgttctgt cctccttgcg
    15781 gagtggcaat catttatctc cggccatagg ccgccagcga attcggttcg atttccgatc
    15841 cgccatgccc ctaccaatat ttgggtttct agcttgcggg gtttaccgcg tttcatccgt
    15901 tgcttttcgc aacgactcgt ctctgtggca ctttacggat accggggcct tttacatccc
    15961 tttttccgcc gtcaccaagc ggtgcccaaa cttatcggtt cgtttggcgc aaacactaca
    16021 ggcatcacag cctgtgctag catggacttt cctaacacct aagtgctcga ttgcccggtg
    16081 gtctgacaat aatattatat cacttttgtt ctttgttgcc tatggagtct aaaactgcct
    16141 ttctggaaag gtcgacgcgg cctttgtcgt cgatgccgat cactttgacc gtgatcacat
    16201 cgccaagttt gacgatgtct tcgacttttt caactcgttc tttggccagt ttggatatgt
    16261 ggaccagtcc atcctgaccg ggccacaatt cgacgaaaca gccgaatttc tcgatcctga
    16321 cgacggttcc ctgatagact tcgccgactt cgacttcgcg gatgatgtct tcgatcatct
    16381 tcacagccat gtcgatgaag aacatatcct gatgcatgat cgtgacgcgt ccgtcttgtt
    16441 cgatgtcgat cgttacctgg ttgcactttt cgatgatttc attgatggtc tttccgccgc
    16501 cgccgatgat ctcgcggatc ttgtcgaccg gaaccatcat cattctgact ttcggagcgt
    16561 acttggaaag ttcggctttc ggtttggcga ttgtcgcatc catgacatcg agaatctcca
    16621 tgcgggcctt tttagcctgg gcaagagctt ctttcatgat ttccctggtg atgccgtcga
    16681 tcttgatatc catctgcagg gagcagattc cgtttctggt accggatacc ttgaaatcca
    16741 tgtccccgaa atgatcttcc aaaccctgga tgtcagtcag aatagtgtaa ttatcacctt
    16801 ttttgactag tcccatggcg atgccggcga cagctgactt catcggtaca ccggctgcca
    16861 tcaaagcaag gctaccggcg caaatcgtcg cttgcgatga tgatccgttg gattcgagaa
    16921 tttccgacac aacgcgaatc gcatagggga attcctcttc cgacggcaat acctgcaaca
    16981 aggctctttc gcctagggcg ccatgaccga tttcccgtct tccgggactg ccgtaacggc
    17041 cggtttcgcc gacggagaat tgcgggaaat tataatggag catgaaccgt ttgccatcct
    17101 ccaccgacac accgtcgatg atctgggctt cgcccagggc tcctaaagta acggtagcca
    17161 gtgcctgtgt ctgacctcgc gtgaacatcg acgatccatg tgtgcgttcg aaaaggtcga
    17221 tctcggcgga aagcggcctg atctcgtcaa gcttacgtcc gtcgggacgg attttctcat
    17281 cggttataag tcttctgact tcgtcgactt ggattgtttc caacacccgg tcggcgaatt
    17341 tgattttttc ttcgatttcg tccttgtcga atccctgaga cttgtattcc ccggtcaggg
    17401 tttcgcggat ttcctcttca atggttccga tcgcatcgcg gcgggccagt ttctccggaa
    17461 ttgtgatcgc cttgattata cggccttttt ccagttttct gacttctttg tcgatatcat
    17521 ccggcacggt ggatagttcg atttccattt tttctttgcc gacggaggtg atgatttctt
    17581 cttcgaaagc caccaattcc ttgatcttgt cgtgggcaaa catgattgct tctaacatga
    17641 cgtcttcgga aagttccttg gatccggatt cgaccatgtt gacggcatat ttggtgccgg
    17701 caactgtcag atcgagatcg gaaacaacca gttcctcggc ggttgggttg cagatgaatt
    17761 tgccgtcgat catcgcgacc acgacaccgg caacggggcc gttgaacggt acgccgccaa
    17821 ttcccaaagc gagggatgaa cctagcaagg ctgccacgaa tggcgagttg tcgggatcaa
    17881 ccgacatcac ggtgtcgaca atctggactt cgttgcggaa atagtcgtca aacagcggac
    17941 gaagcggacg gtcgatcgcc ctggatgtaa ggatctcttt ttccgtggga cgaccttccc
    18001 ggcgcagaaa acctccggga atcttgcctg ctgcatacag tctttcctgg tagagcacca
    18061 tcaagggaaa gaaatcctgg cgtgagggtt ccttgccgac gacggctgcg gacaaaacgg
    18121 cggtatcgcc cattcttatc atcacggcac cggtagcctg tttggcgtat cttccggtct
    18181 ctaccgtgat tgtttttccg caaaactccc ttgtaaaagt ttgattttca ctcattatct
    18241 atgtcctcat ttctgaataa tcggttatta taaaaacatt ataccataaa tcgtataaaa
    18301 caccggtctt tttttcaaat aatgagcacc caataacttg ggtgctcgaa gtcttataga
    18361 ctagcggcgc aagccgagtt tcttgatcaa cgcctgatag cgttccatgg aaaccgactg
    18421 caaatactta agcaaacttc tgcgttttcc gactttcatg agcaggccgc ggcgggaatg
    18481 gaaatcatgc ttgtgaacct gaagatgttc attcaaggaa ttgatttctt ccgtcaggag
    18541 cgccacttga atttcggggg aacccgtgtc cccttccttg aggccgtatt cgacgacgat
    18601 ctcatggact ctttctttcg taagtgccat aatgttctcc tccttcttta gacttacccc
    18661 aatccgcatt tgacgtcgga gactcgtcaa atccaggttc gggcgcgaga aatattatat
    18721 cataatgcat gtctatatgc aaggaatatt tccttactcc gccaaccttg agattatgcc
    18781 tttcagaatg acgaacagcg acatggtatc ctggttgcaa tacaagataa ggtcttccct
    18841 gatttgcatc ctgttttcca tggatagata cggtaaatag gcatattgca gataggcttc
    18901 caatccgttg gcgatcgcca ttcccttgta ggcgtcctcg gcgaacaccg gtaaaacgct
    18961 tttgagcgaa taactgccgg aaaggtcggg atgatagtag ttgtaggctt tggcctctgc
    19021 tttggaatat cctttggcaa gataaaaagg cgaatcgttt ttcaaaactt ttagcagatc
    19081 gaacaaccgg tctcggatat tatcgagtct ttccttgtac accgggaaaa gttgagcgag
    19141 ttcctgtaga cggttgtcct cgaaagtctg gttgtacaca atgaccgaag aattcccgtc
    19201 agggatcact tccaagagcc tttcgaccag tttgcggcga ttgtcccggt ctgaaggcat
    19261 cacgtaatca taatggatca aaccggattt gtcggttttc gaatctttct ggacatgtac
    19321 ggaaaactgg aatacgcttt gcgtataaca cttctctccc gtaaaacggg gaatcggaga
    19381 aggataagcc tcgaaatcga gaaaataaag cggatagaca agcgattcaa gtttgtcgga
    19441 gatcttcttc ctgttgatga attcgtagtc gttttgtacg cagtatcttt gcataagatt
    19501 gcattcgcgc tgcaaccagg agatcggaac atcaagcatg tcgaccattc cggaattcac
    19561 gagttcatag gtttcatgga ccggatcggt tctttttttc ccttcccgaa aacccaggtg
    19621 ttggtgaaaa tacgagaata tggaattctt tttcggaacg tgttcgaaac agaaatcgac
    19681 atatggacag gcaaacgccg tatgcggcag acattcgttt ttgacgagga ggcaacggga
    19741 gtcgtcgttc agtttgatgt ggttgatcat ccggtacaaa tccgtttcca ggatttctcg
    19801 tgctgccggc aacaattccg agattttgaa gaaggaaagc atttccggct ccggctcaag
    19861 cttgttgcga acatattcgg aattgagaag aacaagaaca gtttcggttt ttttgttgga
    19921 atcggatttg tcaaagacat atgcccggaa cagggcatca taaagcaaac ggcccgattc
    19981 cagattccgt gccatatatt tcgccatgct gaaaccggct tccgtgatca actggcggtt
    20041 aggtttatag gtctgatcct tgattttcgt gaaaagcgga cggcggcgcc tgccgtcgat
    20101 atcgcatatt tcggtgaggt tgtggcttgt ggccgcactg atcaggtaat aggtgttttc
    20161 attttccgtt tcgatcatgc gggctgccca tgcgtaaagg gtgaatcttt cttcgaaagg
    20221 aatctcgatc ctggcttccg gcttgtttct ttccttatcc ggataaccga tggcggctgc
    20281 cagggaagct agaatatcct cacgggcttt ttcccgtaaa attctggtga ttttggcggt
    20341 atcgggatta tcaagtcttt ttgattcata gtcaccgttc tccacgggga tttgaccaaa
    20401 atcgacgggt aaaaccgtct tttcttcctg atcgtcgtcc ctgtcctgat gataattcag
    20461 ggcagcataa cgataacagc gtatgtagtt catgagtttc gtgctggtta tcctcatcgg
    20521 ttgactcctc tggtaattcc aaatctatta tatcacaaca taaaggcgaa acatcgctga
    20581 aatatgttca tatgatgttc atcatcgcga tgaattccct ttcttcggtt ttagctcctt
    20641 ccggatcttc tcccgcataa tcgtctaaaa gcatcatcat gttatctttt cttatccata
    20701 ttgcctcgat attcctaggc aaaggaaaaa acaggggtgt ttttccctct aagacataat
    20761 aatagctgat cgtcgactga ccctcgtcat gtcttgtcag aatcagggaa agccgggccc
    20821 cgcttttttt tatggcatcc gcgaatgttt cgtcttccgt ctcggcaaaa ggaaaatata
    20881 atagcccgtc cccttgacgt ttgaaagcga gaaccgaatc accgtcagtc attatcaggt
    20941 gtagataggt tttcttccga ttgttcctta catccttctt tgtcaggttg tctgtgtata
    21001 tttcgatttc catccaatgc ctccaaaaaa tgaccgaccg gcttcttgag ccagtcgatg
    21061 acaaatccga tcccaaggcc gtagattacg gtcgtgggtc cgatataaaa ccattctgac
    21121 tgatggtgaa aatataatcc ggcgaaaatg gtcaaaatca atgcgatgaa ttcgccgatg
    21181 atcttggctt tgccaaagga aacccggaaa agtttgtgca ttccgtaaca aagttcgtca
    21241 agcgccggca aagggtattt gctgtaaagc aaaaaattga tcccgacggc gcaaatgatg
    21301 atggcgataa acagatacat aagattccag aaaagatttt catctacgac ggacggaatc
    21361 accaaaagca aaagatcgat tacgaaaccg atgtaaaaac tgataagaac cggaaagagc
    21421 atccaaagat ccggtttttt cagttggatg agataagcga tcgaaatgat gacaagtgcg
    21481 aaaatcggtg tcagatattt gtattcgatc ggaatccctt cgtaaaaatt ccggttcagg
    21541 gcgtcccagc tcgacatccc gagattcgtc ttctggataa acgccacgtt aatgccgaga
    21601 aaagtgagac ccaggatgta gattatcgtg ttcttgatcg tgaattttcc gcgcatcgcg
    21661 ttgcctccgc cgtcttccgg tatctttttt tgattatacc acaatcatat gtgtttttcg
    21721 tcacgtggtt tatcaaaaaa ggccgtccgt ttccggacgg ccacattttc aggcgaccag
    21781 ggcgatttta agaacgtctt caagcgtttc ggcaaaatcg atttccaaaa cgtcacggac
    21841 ttccgaagga atgtcctcga catctttttc gttacccttc gggatgacaa tcttctttaa
    21901 cccgctgcgg ctggctgcga tggctttttc tttcaaaccg ccgatgggca gcactcttcc
    21961 tttgagggtg atttcgccgg tcatgccgac gaagcgattg accttctttt ttccgaaagc
    22021 ggatatcaac gccgtggcga tggtgacgcc ggccgaaggg ccgtctttcg ggatggcacc
    22081 ttccggaaca tggatatgga tgtcggtatt gtcgaatact ttcggatcga tgccaagctt
    22141 ttcgctgttt gatctaacat aagacaaggc ggccatggcc gattccttca tcacgtttcc
    22201 gagattgccg gtcaggacaa ggttgttttt accttcataa tacgtggctt cgacatctaa
    22261 agtgtcgcct cctgcgatgg tgtaggcaag tccggtaacg acgccgacca tgtcttcttt
    22321 ctgcacgccg ttgttgaaga aaagcggttt gccgagataa tggtaaagat cgtccggtcc
    22381 gacggtgacc ttttcaagtt tgtcgaccag gatttccttg atggcttttc tcgtgatcga
    22441 accgatcagc ctttcaagct gacggacgcc ggcttcgcgg gtgtaatctt tgatgatctg
    22501 gcggatcgtt tcatcttcga tttccagttt tccctcttcc agaccatgat tctcaatctc
    22561 tttcttgatc aggaagcgtc tggcgatatg gaatttctcg atttccgtat aggatgagag
    22621 ttcgatgatt tccatccggt cgcgcagagg agcgggaaca ttttccagat agttggcggt
    22681 tgtgatgaaa agaacctggg atagatcata atcctcctcg acatagtggt cgctatagaa
    22741 ttgattctgc tcgggatcca aaacctccag catggctgaa gacgggtcgt tgttctgatt
    22801 gatgaccaat ttgtcgattt cgtcgatcag gaagaccgga ttagccactt tggctctgat
    22861 catgccattg atgatgcggc ccggcagggc gccgacatat gtgcggcggt ggcctctgat
    22921 ttcggcttcg tcatggacgc cgccgagact tgtcttcaca aattcccgat tgagagcgtt
    22981 cgcaatcgat ttggcaagcg aagtctttcc gactcccgga ggaccggaaa ggcagaggat
    23041 ggtttgcggg tttttcccgg tgagcatctt caccgacaga tactcgatga tcctttcctt
    23101 gactttttcc aaaccgtaat ggctggattc aagtttctgg atcgcgacat tgatgtcctt
    23161 ttcatcttca gtggttgtct gccagggaat cgaaataagg gtttcaagat aagtcctgac
    23221 gacattcgat tcgctggaat tagcggacaa atattcgtaa cgttttaatt ccttcagggc
    23281 tttttcgcga actcggtcag gcatgtcaga agcattgatc tgttccaaca gtttggaagc
    23341 atcatcctgt ttcgaatcgc caagttcttc ctggatcgcc cgcatttttt cacgcagata
    23401 atattctttc tggttctgat cggctgaacg acgaaccgat tcgttgattt tcgattcgat
    23461 cgtctggaga tttttctctt tttcgatatc ttctagaatg tattgaagac gcttgtttat
    23521 gctgtttgta agaaggtact ttgcacgttc gttttccgat attctcaatt cggaggcaat
    23581 caaatcgcag agtttgtcag aggtgatgcc tccctgcatg gaatccaaaa cttttttgga
    23641 atcgcggaaa aggaaggaag cgttctcgac cgtttccttc gataccattt ttagcagaac
    23701 ctgttcttcg tccaagtttt ccggctgaag caccatggtg gtgaaatcga tcagatagaa
    23761 cggttccttg gcaatgagat cgtgaagttc gacccgaccg atcggttcga acttgatttt
    23821 gaaattgccg tttggcagtt tgattttgac ggatatgcgg gcaagcaacc ccacgccgag
    23881 aaaatcggtg aattcgggtt ccagaatttc cggattgctt tgaaaaacga gcaggacgtg
    23941 tccgtcgcga ttgttttccg cctcatagat ggcatttttg gaaaaatcgc ggcccacttc
    24001 tacgcggacg tcggatccgg gcaaaaagat aacgccgcgg acggggacga ccggaagccg
    24061 gtcgctgtat agtttgtttg cttcatacat cggtaatcac ctcgcaatta gaagcgaatg
    24121 aagtgcagaa ttcggtgttt tacgtattta ttttatcata tatttgtgat gtttgtaaaa
    24181 agaattacct gttaattaac agaaagaatc ctaagcgtat gccgcctagg attccgaaaa
    24241 tcgatctgct ttgcctttta tcggatgaca ttcgcctttt caatcaaaag atcgatggcc
    24301 tttttgaaag aaatctcttc cttgatggct gagacgggga tagcgttctt gacctggtca
    24361 agggtgatat tctgactctt gtactggtca gcgatttcct ggtatttggc ttcggtttcc
    24421 ttgtcgtcgg cttccaggtt ttccttcttc gcgatggcgt tgatcaccat ctgctggcga
    24481 agcgtcttcg tcgcctcttc cttgagttcg gtaagatatt cttccttggt ttttcccata
    24541 tatttgagat aagtgtcgaa atcgagacga tattgcttca tccggttctg gttgtcctcg
    24601 tcagcgcgtt tgacctcttc ttcgatcatt tcctcaggca aatcgaaagt agcgttttcc
    24661 gttgctttct cgatgacggt ggcacggatc tggttttcgt tttgtttttt cttgtcagtt
    24721 tctatttgat ttttcacgta tgccttgtat tcatcgattg tagagatgtt gtcgagattc
    24781 aaatccttga cgaattcgtc gtctagttgt ggcaccacgc gattcttgat ttcgtggaga
    24841 gtgactttga ataccgactc tttgccagca aggctttttt catgatagtc ttccgggaag
    24901 gatactatga cattcttttc cgtttccggt gccattccct tcatctgttc ctcgaatccg
    24961 gggatgaact ggttggatcc gatcactagt tcgtagtttt cggcggatcc gccgtcgaat
    25021 ttgactccat cgacactgcc ttcgaaatcg aatactgctg tgtctccttc ttccaggacg
    25081 ccctcttctt tcaaaaccat ttcggcgttc ttttcccgtt cacgagtgat ttcggtatcg
    25141 atttcaacgt ccgatgccac atcaggcaac agtttgactt cggttcccag gtattctccc
    25201 aaaacgactt ccggtctgac ggccacggtc acttcataag tgaaatcgga tccgcgcttg
    25261 acattcttga aatcgaaatc gattttcggt tgggcgacaa cgtcgatgtc gttttcggta
    25321 atcgcatcgt aataggtatc gccgatggcg tgctctacgg cttcttcgta aagggattcg
    25381 atgccgtaat gggcttcgta aacggaacgc ggcgctttgc ctttgcggaa gcctttgatt
    25441 tcggtctttt cgttaaccac ttcaaatgca tggtcgagtg catgctcgaa catttcgccg
    25501 gtcaccgtta tcgtgaaatg gacgcgagag ccggacagct tgtctatcat cataatatga
    25561 tcctcccata tttcaatcag caaacaacat tataacaaaa gcaaggccat aaagcaaact
    25621 tttttactcg tcagaacacc ttttcaaaga tggatttaaa ccgagatggc aatttcttca
    25681 ccgattcggc aaggacgccc ggaagcaggg tcaccgcttc ggcgaaagcc agtttgcgga
    25741 tcttgttccg gtctacccct ttcaggttca tgaaaaggta attccggcag atgattccta
    25801 ctcggagcga aagcaaggcg ttgccgactc cctgagcgaa ggaaccgata atcgttttca
    25861 ggaaaggcac ttccgacaaa gcggaaaggc ctttgcttgg aaagagctcg ttgaaatcga
    25921 tatcttctag actttcggcc actacagcgg ccgtcagcac gttgaacgag agacgaccaa
    25981 gggaagcgta actgggacga aatccacatt ttagtaccag ttcttttatc agtttcagat
    26041 tgatagtgaa gaccgcgatc gaatcaagac ggccgttctg gctgatggcg gtcgacaaaa
    26101 acaccgattc ggcatgacgg atgattatct tgttaagctc ctttttgatc ttctggtcga
    26161 aaacgaaaga gagactctgt ttaagcttga ccggatcgga taacgaagct tggagcgatt
    26221 ccttttcttc cttggccaaa tattcttcct ttaacaggtt gtcggagact ttcctgtaca
    26281 tgtgatagtt ctttctggcg ttttcttcgt cggtgaacag actgtccatc gagaatgtgg
    26341 gcgaaaagat gattaaaaac accggtctca cgatcaggat gtaaaacagg attaccgaga
    26401 tgccatagaa tgcataggcg acatacggat ggattccgtt caacttgtcc ccgatgttta
    26461 atacgctgga tatgatgatc aggatggcga agaataatgc cgcgatcccg atggttatcc
    26521 agaatgcttt tttgaatttg gtcattttat cctcccgttt tcaatggctt ttttcaattt
    26581 catttcatcg gcttcgactt gtttttttaa ttcttccgga gtgtcaaaac ggatttccgg
    26641 ccgcaagtaa gccaaaaaat ctatcattgc cgtttcattg tatgattccc ttccgaatcc
    26701 aaaaagatgg ctttccagtg tcatctgcct atcggagaac gtcggattat caccgatgtt
    26761 tgtcagtgaa aagaatgatt cgttctcgat agttgttttg gtcatgtaca cccccggaag
    26821 gggcaaaatg tagtctttgt aatcgacatt ggcagtagga tatcccagga catgtccccg
    26881 tctgtggcct tttacgatgg ttcctttgat ccggtagggc cggccgagaa gattgttcgc
    26941 cttttcgatg tctcccgatt ccaagtattg tttgatcctt gtcgatccga ttttttctcc
    27001 tccggattgc atttccggaa ccacttccac tttcccggga aatagttttt caagcaattg
    27061 cggtttgcca ctgccatgcc ggccgaaaga aaaatcaaaa ccgacaatga ttgtcttgca
    27121 ggatgaaagg tatctgaaag cgaagtcttc cggagtcaat tcgatcaggg ccctgtcaac
    27181 ttccagaacg atgaaataat ccaagccgta cattgaagcg atttcttctt tgtccgacaa
    27241 tgtggtgagc atctggtatt tccggtttcc gagtaaagaa ggaatgtgct cagacagggt
    27301 tagcatcccg ttgggatgcc ccttgtccct ggcggtttct agcagctttt gatgagcgag
    27361 atgcattccg tcgaaaaaac cggccgccaa tgttaaattg ggaatttcca atctggcagt
    27421 cgaatcgaga atgatcttct tcatagcagt ttcacagcca ttcgcatgac cttcctttcc
    27481 ggatcgaatc ggtagatggc caccggtttt ccgtatttgt catagataat tgtgtcgctt
    27541 tcatccggaa aatactcccg ttcgacaaac gcgccgttgt ctatcttttt ggttatgtcg
    27601 tcggacatga cgatcttgct aaaacccatg ccggccaaag gatcgattat tgacaagttg
    27661 ccttccatta atttttcgta tggtatggcg tctgtcacac tgtatggtcc cgaagttatc
    27721 ctcttcaggg ccacaagact cgccggtatg ttaagataag cgccgagatc cttggcaagt
    27781 tgcctgatat aggttccttt tgataccttt acagtgaaag agacttttat tttacccgct
    27841 ttttgatcct tgacgtcaat attttcataa ctgtagacgg atacctcgcg ttgaggcatt
    27901 gccggtatct ttaggccttt tctctggtac tcgtataatt tacggcccgc caccttgact
    27961 gccgaatacg caggcgcttc ctgatagaaa gtacctgtat aatgttctag ggctttgaag
    28021 attgcctctt tgcgaatatt ttcgcagttt tcttccctga cgactttacc cgacggatcc
    28081 aaggtatcgg tttccaaccc gagaatcatc gtagcctcgt aagtcttgtc gtgattgttc
    28141 agatatggca tcagtttggt ggcccgcccg atagctaata ccaaaagccc ggtcgcaaaa
    28201 ggatcaaggg ttccggtatg gccgatcgtc tttgtcttga gaaccttccg ggcttgattg
    28261 acaacatcgt ggcttgtcaa accagccggc ttctctacca aaagaagtcc gaactggttt
    28321 ccgagataat ccatctctag aagacctcgg aagaaagcaa ccagcttctc agggcatctt
    28381 tactgtgaaa gccttcgatt cgttgcaact cgatgccttc ctgaatgaag acgacaagcg
    28441 gaaccgcttc cgcccggaaa cttcttgtga ccatgggcat ctgttctgta tcaatcgtga
    28501 tgccgttgaa atgcatttcg ctttccagtt cggaaagcca tatttcctgt tgttggcagg
    28561 cggcatccca cttcagcacg aaaaggatga catatctgcc ggtatccagt ttgagttcgt
    28621 gttcgtttag cggcattttc atgttttcat gcctccattc acttgaaata gatgattgtt
    28681 accccgtggc cgccttcgtt ttggcccccg gatcgtgatt ttttgatcgt attatgtgat
    28741 tttatatact cctcgactcc ttttttcaag gcaccggtgc catacccgtg aatgatcgag
    28801 gcgaattcaa gatgatgaaa caggcaatcg tccacatatt tgtccaattc ttccatagcc
    28861 tcttcgtatc gttttccgcg aaggtcgagt tcggcgtgaa tgatgccttc cttggaaact
    28921 ctgacttcgg aacgcggttc cgactggtca gcctttttct gatattcaag atccgattcg
    28981 gaaaagaccg ccgacaagac gcccaattgg acttcaaatt cttttgcgtt tctttgtttt
    29041 gtgacgacgc cgatcctttg atagggaatg acgttcacta ggtcgccgat ttttattttg
    29101 ctttgatcga ctttttggta tttttcttcc accgtgtcca tatttctgac cttgtatttc
    29161 atattggcca gctcatgttc tttgacattg gcgtttttct tcataccatc taattccctg
    29221 atcagttcca tggcttgttc cttggctttt tcaagctccg catgtttctg ttccttcaat
    29281 tcggaaatga tcttgttctg tttggatctt gattgcatta tttccgtttt cagggattct
    29341 tccaggctgg caatattagc ctgcatttcc ctggttttgg cgatttccgt ttccaaatcg
    29401 cggctttcct tttcaagttt tttgatcagg tttgtcgttt cgttgtcaaa agtcagggat
    29461 acggttttag ctttttcaat tatcgattct tcaagcccca gacgtttggc aatctcaagg
    29521 gcattcgagg ttcccggaat tccgattttc agtctgtatg tcggtttcag ggaatcgatg
    29581 tcgaattcca cggaggcatt gacaacatcg tcgagattgt aggcatatac ctttaattca
    29641 ggataatggg tggttaccat ggcgtggcat ccgacttggc gtagatagtc taatagactg
    29701 atagctaaac tggctccttc tttcggatcg gtgccggctc ccagttcatc gaa
//
LOCUS       DAOPJL010000014        28948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1724_length_28948_cov_9.481051, whole genome shotgun sequence.
ACCESSION   DAOPJL010000014 DAOPJL010000000
VERSION     DAOPJL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 28948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28948
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1724_length_28948_cov_9.481051"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1300
                     /gene="thiI"
                     /locus_tag="PLP02_03030"
     CDS             74..1300
                     /gene="thiI"
                     /locus_tag="PLP02_03030"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPF42458.1"
                     /translation="MKDLLMIRYGDLILKGRNRNSFMDAVNRLIKEKLAGLSVGLEFC
                     HDRAYVHLNDAPLDIVIRQLGYVTGLYSYSLMSKCQVDFDEIAALAIELLTEDTKGQE
                     ITFKVETKRVDKTYPMQSPDISKVLSGMILSEMSNLIVDVHNPKLTLTIEIRKEGAYL
                     FTDQIKGLGGFPIPIAGKAVVLLSGGIDSPVASFLAMKKGFQVELVHFESTPMTSIES
                     AQKVIDLAEILSRYAPHDEIRLNFVPFTAIHEKIIASVPESYIITIMRRMMVRIASGI
                     KEKTGSLAIITGDSIGQVASQTPESLATIQEAINDLVIRPLSTYDKMEIMEVARKIGT
                     LEISNRPFSDCCTVYIPKSPVIRPSVAAAVKYEESFDYRPMIEEAVNKTKTVTIIADK
                     HLDIQSKGLSIKDVLS"
     gene            1297..3591
                     /locus_tag="PLP02_03035"
     CDS             1297..3591
                     /locus_tag="PLP02_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPF42459.1"
                     /translation="MKTIETKRLILRTWAQADASDMFDYASLATVGPSAGWKPHETIE
                     ESQRIIAGFIEKDDVWAVELKENHKVIGSVGMHERQTIGGIITKELGYVLSTPYEGKG
                     LMTEACKAVIHHVFHETQVDVIAVYHFLENDKSRRVIEKCGFIYENIIEYKTSTGDIQ
                     TSKSYKLTKESYLKMEELRNYTWNLDKLYTSFDSKKFADDNLALENLVDRIKAKQADF
                     ANYDEKVSKLKSYLELNIEITVLAIKLMNYAGLQGATDSTDQQAAKSENRLHVKLAET
                     AAVLAASAKWIRNFPELDEAIEADPFLKEHEFMLKEIRENASHLLDDKTEALLAQLKP
                     SGSTAWERLQALLTSTVAVDYEGKEITLSQVRNLAYEKDPKVRENAFRAELKAYEKID
                     KPVAFAINAIKGEVNVLSAAHGYESALAEALAKSRMQRATLDVMMEAMEEYLPIFRKY
                     LKRKGELLGHKNGLPFYDIFAPMGKSEKTFTIPEAQEYILRNFRTFNERLYNMAKRAF
                     EEGWIDYLPKKGKVGGAFCSNIHPIGESRVLSNFTGVFGDVITLAHELGHAYHGECIF
                     GESILNSEYTMPVAETASTFCETIVNKAALKDAVSKDEKINLLESSIQDYTQVIVDIM
                     SRFIFEKNLFEGRKKTIFDENELKEMMLDAQRKTYGDGLDPELLHPYMWLCKSHYYSG
                     NLSFYNFPYAFGLLFAKGLYAEYLKDKEKFVMMYDSLLAATGKNTVEDAAKIAGIDMT
                     KKEFWVSSLKLLEEDIEEFIRLTE"
     gene            3660..5642
                     /locus_tag="PLP02_03040"
     CDS             3660..5642
                     /locus_tag="PLP02_03040"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPF42460.1"
                     /translation="MKKILTGALLVLLLIGLAGCQKSNGWEAVDFDNVEGVGQFESYD
                     QLKDYLDSYYEEDTLYYRTMDSNSAALESDIMTTTTAQSDSTDDVNYSETNNQVNGVS
                     EADRILTDGYYIYVLSDNGFYIVDANTLEFVYVFTNDYGYAYGLYKYGDSVVVLMHEY
                     YYINYMIEDETTTVTSGASDSDIDGSSYVYLNNYGTRVIVFDISDKENVTVTRNLFFE
                     RSYLNDSRMIDDVCYLMMENYAGRYGYDEDDYFPVFYDDAASDKAIDLPIDHIYYMPG
                     NNYYISYMLLATFSVDNDEQADVNAYLGSSSDLYMSLDNLYVVCYRYSYTQVFLTDAS
                     NDETVFSNWYFQYWTYVIRFGVEDGKLEYEAVAKINGYPLNQFSMDEYDGYFRIATTG
                     YVYSEDYSTSTITNQLAIFDATTEGEIEPVSVLTGLGKPNERIYAVRYSGNIAYVVTF
                     VNTDPLYKLDLSDPENPEIVDELYEEGVSDYLHEIGDNLLLGIGRQAVTENGFTHFTG
                     VKVALYDVSGDETINLETYFVEGDYSYSPVTYDHKAFVSYEPEGADYMYVTIPVYEYY
                     SDWYASLQSVYVFKVYYAGDLEFITRLTHMDDTSHTYWYHYDSIERTVMIENYIYTVS
                     YNQIRKYDMASGFTLDVTNYFHSEDGQYYPYYGFYD"
     gene            5807..9940
                     /locus_tag="PLP02_03045"
     CDS             5807..9940
                     /locus_tag="PLP02_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42461.1"
                     /translation="MKRRYFALLLFLLFGMFMVACGNDTLPTTNLTSNTTSATTLTTG
                     STTVSTTASTTASTTTTVYTGETVTVRFLDGNDVLISEVVIHKGASVVPPTPPTKAAT
                     TYYSYVFSGWDHALTNVNEDTTIRAQFVATYLFTTGTFNHADLLTLIRNLTDDTASSD
                     AEIEAKIAMIMTMIGISSEEHLYDLLSEVPDLMTSFQAITSSSEFATWYAQLKAAGFN
                     KELITDTAINLIRNGVGMWIENYDPEDFADEILGYQNQISVNEAAITDIQTAVNLYCT
                     LYTSDPANCQLLFSYILDDFYNYINYDNMYYSYFYSVDWEYSDEYYNLQNYMDSYIYF
                     SYQEGMETEAANALSEYNNYYSSLTEYQQNAFDPIIDAFSTYSEHYWAYTTPLTNDLS
                     GLTDTQYGENVVQYLYSQEQSIENLYWTNHSLYQDIANVQDEMVRMEEQFMMFQTLQQ
                     YLQTQDGLDNLKILVIAFYDSLDAIATAPNADLFNLITTLISEGEEFDPSTITTSDAL
                     AYLVGLVDMMEIVQTNMLSTASLANIIEIAEDLMPQFINNMDDLTSVEKAALIATITP
                     KITQYVNMFASEYDNVLTFLDSMNFVKGEAVMDFVAFMSEYDEQNDTQIGGEYPVHMI
                     YQVSVLVNTLIGDDSFDVSQLLSDLIVVYYDIDTEFQAVPSEVTDTQAAATANVERII
                     VLAGIIAAGDPDNLSPTQMASYVEIGIRVQAFITMFSDGFETALEPIVTTYVHGALVE
                     AITHMFDVSETEAEGIITDMLVIMGFEDEEDMYYFFVSVATHAQEMEIGINNYTDLES
                     WINWFYDMGYTKEEIADIVYEMAVYVIDLRIATGGILENDIAYYENQILVYEGLIVDA
                     NAAIDSLSGEFTGMLGMASPEEVDLFMNFWTLQKEKSMREYTIMYMEYDVDYEYFDWG
                     TYDGLKTYLYEDYIGTPGAMDSYNSMFYSLSPDEQAAYQPLMDQWMAYYDFLPTYESS
                     QSEVSSTSNLTDAYIFVMNNLQYSYENQLSNIFSYESQITWMTEKIDDLNGQMELLTV
                     LSEYLANTADGKDELVKNVIMIALDEVENLLSATDDATIDMFIALIQNLSGMPDNGFD
                     PATISASDIYNYTQEAANILTTLLATIDSTDQANIRAFVLDALEIKWTAEGEMTPTEI
                     SDQLTIIGSTFDKYYPRLVTILGMLSTALIQLDTTEIGDIMDTAGNIAGYDNSIAMMI
                     VYVSEIADIFLTDTPTALDYTTLVDYAVDAYFDITYDFNYTGEVNIADLQDVYEQYAA
                     SIAAYFHLIAAMDPDNLTTDNVKLIFSSYLYVRGLVGEIEEGGFETMTYTAPDMMYPY
                     YTYDYQDFYDLVDDMIGGDMTETEINDWISDYVTDSGLTAEEAMYQLFMNGLGEPTE"
     gene            10025..10741
                     /locus_tag="PLP02_03050"
     CDS             10025..10741
                     /locus_tag="PLP02_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF42462.1"
                     /translation="MIITSADNQKFRSYLRLKQKKYRYQTGLFLAEGPHLVNEAAKAR
                     RLEAVVSTEPFTDIKVDNLVVSASLFASLSDVETPQGVIGICKMADNVLLGDRILMLD
                     GISDPGNLGTLIRSAKAFGFKTFVFENIADPYSPKVVRATQGAIFGLGFLKADLIEFI
                     GKHREYEYVGTDVKTGLPLSEFNPNGGKLALILGNEANGVREEILNKTGVNIKIEMDG
                     MESLNVGVAGGILMYALRRK"
     gene            10744..11040
                     /locus_tag="PLP02_03055"
     CDS             10744..11040
                     /locus_tag="PLP02_03055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42463.1"
                     /translation="MEFRYEEEKIYLLDESRKTIGEIDIPKRDEKRIDITHVFVDESL
                     RGQGYAQALMQTAYDYIKKQGKLIVAKCPYAIAWFKKHYECQDIVINVKTGPKV"
     gene            11145..11582
                     /gene="rimP"
                     /locus_tag="PLP02_03060"
     CDS             11145..11582
                     /gene="rimP"
                     /locus_tag="PLP02_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPF42464.1"
                     /translation="MDLALLKKQVGQEIEKYGYELYDLIYHKEKKQNVLTIMIDKPEG
                     ISIDDCVNVSHRLSDFLDGIDPIQDAYSLEVSSPGAERELRNDDEIEKAIGKYIHIET
                     YEQKADGELIAYDGDVITIRGEKNKQMSFGRLDVRTIRLAIKF"
     gene            11595..12659
                     /gene="nusA"
                     /locus_tag="PLP02_03065"
     CDS             11595..12659
                     /gene="nusA"
                     /locus_tag="PLP02_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPF42465.1"
                     /translation="MVSKEFFKALEQVAEERDIPKEKILDIFGKGLLNAYKKDYNGQQ
                     NAKVVFNEEKAEIIIVATYLVVEEVDPEAEKGTQITVMEAQQLKKNAKAGDIIEVKVT
                     PKDFGRIAVSSAKQILTQGLKQLERERNFEIFTDKTGEMVIAEIVAVNDEFVTLYLGF
                     DTETSIPARDLVGTEIFVGNQIRVYITKVEQTTKGPKVYVSRTDRNLVKRLMEQSIPE
                     LQEGVIEIMGLARDPGDRCKVCVASNNLRVDPVGACLGRNGARIHEVIDALDGEKIDV
                     YKYSEDPAELVSNAIQPAKTTSVIVDQREKSAVIIVPDDQLSLAIGKKGQNARLAVQS
                     SGWKIDIKSETDAMIQGIKY"
     gene            12679..12951
                     /locus_tag="PLP02_03070"
     CDS             12679..12951
                     /locus_tag="PLP02_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPF42466.1"
                     /translation="MATIKKIPLRRCVVTGERCEKKNLFRVVRTPEGQVIYDPTGKAN
                     GRGAYVSRNKAVIEKARKSKVLAKHLECEIPDDLYDLLLGRLTSNE"
     gene            12944..13252
                     /locus_tag="PLP02_03075"
     CDS             12944..13252
                     /locus_tag="PLP02_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPF42467.1"
                     /translation="MSKEQVLKTLGLAYRAKKVSSGEEAVLDQLKRGSSFLVLLASDA
                     GGATKKRFIDKTKSHAVPLISNYTAEELSHAIGKEGRKVLSVSDSNFARLLLEKQESE
                     "
     gene            13271..15163
                     /gene="infB"
                     /locus_tag="PLP02_03080"
     CDS             13271..15163
                     /gene="infB"
                     /locus_tag="PLP02_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPF42468.1"
                     /translation="MASAKKPMPKKKSDFKGGKSNQRPANRQTQNNRQTQNYQKPKLE
                     KQPEILEKIVHFKNGMTVLELSEQLHKSVADVIKKLMFMRIMASQTQSVDRDTAELLA
                     MEYGFEIQDEVLTDMTRFDELAIEDDPKDLVERPPVVTIMGHVDHGKTTLLDTIRHSR
                     VVQSEAGGITQHIGAYQVTRNGKEITFIDTPGHAAFTEMRARGARITDIVVLVVAADD
                     GVMPQTKEAIDHARASKCAIIVAVNKMDKPGANQDRVKQELADYGLLSDDWGGETPFC
                     YISALKNTGVDELLDTIQLVSEMLELKANPKRNAVGTVIEAKLDKGRGPVATILVSNG
                     TLKIGDSFAVGTTYGKIRAMNDDLGHTIRKAKPGAAVEINGLNDVPQAGDAFMVFDDE
                     KMTRDIAQKREERQFKEEHNTKKTISLEEFFNKAEGVTKELRLVIKADTQGSIEAFKG
                     SIEKLNIEGTTVDIVRTGVGAITDTDVSLAEASDAFIIGFNVQAPANVRDTAGQKGVE
                     IRDYRVIYEAMNDIEAAMKGLLEPKMERLVIGEAEVRETFSISKIGTIAGCMVTSGVI
                     RRDSQVKLFRDGKVIFEGPIASLKRFKDDAKEVKQGYDCGIMIENYNDVKVGDTIEAS
                     VLKEVA"
     gene            15165..15494
                     /gene="rbfA"
                     /locus_tag="PLP02_03085"
     CDS             15165..15494
                     /gene="rbfA"
                     /locus_tag="PLP02_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPF42469.1"
                     /translation="MAKTDRLDTLVQRVCADIIKNEVKDDLGFITVTGARVTNDLSYM
                     YVYYTIYGDNERREKTQAALERAKGFIRGQIAMQVKMRKVPELIFKYDQSDLEGRKVD
                     EIIRRMK"
     gene            15549..17321
                     /gene="uvrC"
                     /locus_tag="PLP02_03090"
     CDS             15549..17321
                     /gene="uvrC"
                     /locus_tag="PLP02_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPF42470.1"
                     /translation="MNEKIKAKLALLPDAPGSYQMLDDAGRIIYVGKAKSLKNRVKSY
                     FSGSHDAKTTNLVANIADFTYIVTSSELEAFLLELSLIKEHAPRFNILLMDDKTYPYI
                     EITREKNPKLVITRKANTKNRLLFGPYPDASSARETLSLIEKLFPFRKCKALPKKVCL
                     YYHLGQCLGPCEYPVSIDVYKKMENDVAAFLSGRDDQVLDDLEKKMEDCSLNKEYEKA
                     GEYRDLIASVKRTLRKQNVIFNDLSDRDIINYGFYDHYLAVSILFMRQGKIIFSENKL
                     VTFYGEPEEAFISYVAQFYELHPLPKEILLPEGPDYELLEPILNDLGFVPKRGKKKEL
                     VDMAKENAGIYLENNLSPYLRKSDQSIGAIQTLADMLGIQVPVRIEAFDNSNTMGSDP
                     VSSMVVFTNGLPDRKEYRKYKVKTVVGPDDYNTMKEIVYRRYQRMLVEKSRRPDLIVM
                     DGGLGQVNAACEIIASLGLDIPVAGLKKDDRHHTDFLISPNGEEIKLDRHQPIYVLLN
                     KIQEEAHRFAVTFHRDRQSKQVYASYLDKIPSVGKATKEKLLQKYKTLANIAKSDDQE
                     LSEIGLTKVQIKNLRIALADLSSQ"
     gene            17391..18287
                     /locus_tag="PLP02_03095"
     CDS             17391..18287
                     /locus_tag="PLP02_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPF42471.1"
                     /translation="MTEYVYGLLHGVDETKRIIAIKIDNKIRFFYLAKGQFTVFMAYF
                     KPNIYVFMTVAETPRHYRGYKVQNVINIEKVMLPNKQKPTVFYDISIIRSGIKTIVDS
                     GKPKLFVDFEMSMPPYRDYRTFVSEIIQAGLVLCDGDGKVLENHSFFIKPQLFPKISE
                     RTGKFLHIRQEDVDSGREYGEFHSLFMKIQASYHPMVFVWGKNDKIELKKANERLGLT
                     EFTQRAQFIDLLQLHKIYFNLKNDLGLFNAYNLYSDHDLDKQAHDAFEDASVTKDVFY
                     WFKDTVNGNREITIPENGKPAE"
     gene            complement(18325..20007)
                     /locus_tag="PLP02_03100"
     CDS             complement(18325..20007)
                     /locus_tag="PLP02_03100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPF42472.1"
                     /translation="MPNQFFSNNEVGVFALGGLGEVGKNMYCVEYRDELVIVDSGLLF
                     PDEFLMGIDYVIPEYTYLIENQKKIKALFISHGHEDHIGAIPFLLKMVRIPAVYAAGL
                     SIGLIKAKMEEHPGLTINLQEYHESDIIRFNNLSVSFFRTNHSIPDSFGIRVNTPEGA
                     IVHTGDFKFDFSPISADANYQKMAKIGEDGVLCLLSDSTNAELEDFSKSERVVAENIK
                     ELFASIKGRVIIATFASNVHRIQQIVEASSLTNRRIAVFGRSMEKNIEVGLRLGYIRA
                     PHGTFLFSRNMSGLREKNITILCTGSQGEPMAALTRIASGTHKQISIIPGDTVILSSS
                     AIPGNQESINQIINLLYKNGASVITQSPFADVHASGHGGQNELKLMLKLMKPKFFMPI
                     HGEYRMLKTHAHLAVEAGVKPENTFVMDNGDILALSKTGARIAAGKIPTSDVYVDGMA
                     IGEVGSQIIKDRRELSDDGLLSVIVTIDSKSNEIICKPSIVTKGFIFMKDNQQMLREL
                     QEISAKTVSNTLKAETKANVASIKSDLQKAIMNYVQLKTGREPMTLPVVMLL"
     gene            20205..20891
                     /locus_tag="PLP02_03105"
     CDS             20205..20891
                     /locus_tag="PLP02_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42473.1"
                     /translation="MPILTIFLIVLVIVIIFSLAAVIVAIIIASKNRKKRNIIQNNLE
                     KVYIENIVEDKDDCKLTIRDLSGKRETYPVGKKAMQSLYPGETCLIARQNGKLLLWKA
                     QTQEKPDFFSKEKNDTNVMKVTAESPVFDLAISSNSGITASQDEVLGYMDRMLENQTE
                     HFIGLKSDDGRYLELSGNGKDRYLEIRLIKGNRSYRMNKCLLSVAKSLVRDFYGKAEL
                     TDNPGFVIEE"
     gene            20963..23314
                     /gene="priA"
                     /locus_tag="PLP02_03110"
     CDS             20963..23314
                     /gene="priA"
                     /locus_tag="PLP02_03110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPF42474.1"
                     /translation="MIARVLVDVPAKSVDKLFDYLIPAQIAGIIETGMRVIVPFNNRK
                     LMGYCLEIAESSDYDKDLKEIDSLVDLESYLTDELIELAKKIRNETATVLIRILETML
                     PSAMRMTYTTKLRVKDRDRLPDKLVPVFASQDEIILNASQRECYPELKNAIKEGVITS
                     TFVVKSKGSFRMEKVAMLLQKNAVTKSEKQNAVIAFLQEKENMTANAALMIKKLNVTM
                     SVVESLAKKGIILLEYREKYRDLFSLMPVNDKTVILNKDQEVVMEKIRSKYDSFQVLL
                     LHGVTSSGKTEIYLKAIGETIAKGKEVIFLVPEISLTPMMVSRFKGKFGEKVAILHSG
                     LSLGEKFDEWRKIVRGEVKIVIGARSACFAPFRNIGLLVVDECHESSYKQDDIPKYYA
                     IDILEKRAQTYNIPLLLGSATPNIDSYARFKRGYYELLELKHRAMNSSLPDVTTVDMK
                     QEFKNGNSGQLSELLISEIARRLEKGEQTILLLNRRGFSTFVICRNCGHVFTCPECDI
                     SLTYHEADHSLKCHYCNHKEPIPKVCPDCKSEDLRYFGSGTQKIEAEVQEKFPNARIV
                     RMDNDTTRTKNAHEKLLYTFEKKGDILLGTQMIAKGLDFPRVTLVGIIQADGNLYHPD
                     FRAPEKTFQLIVQVAGRAGRRDEPGKVIIQAFNPDHYAIRYARNQDYDGFYNHEMQLR
                     RIAKYSPFYFLAEIKFSGPKMRDLFISAQAVVKLLRSELAEDTVVLGPALPPIPKIRN
                     QYSCQILVKYRHEEHMNDLLNRIRHDFENDLVRIAIDRNPALG"
     gene            23326..24258
                     /gene="fmt"
                     /locus_tag="PLP02_03115"
     CDS             23326..24258
                     /gene="fmt"
                     /locus_tag="PLP02_03115"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPF42475.1"
                     /translation="MKIVFMGTSEFAVPILEGLVKEYEIVLVVTQPDRPFGRKKTLKP
                     SPVKEVALLHNIPVFQPERIKEDFQPILAYGADLFVVAAYGQMIPESVLYYPKYKSIN
                     AHGSLLPKYRGGAPMQRAIMNGDEKTGVTIIYMTKKMDAGAMLAKIEVAITMEDDLGS
                     LEQKLGLAAQKLMLETIPCLASGNVVPTEQDLSEVTFAGIIRPEEEKLDFGRKSKEIF
                     DHVRAFRPKPLCHATIDGKKIKIIEVKIANDIKVEGVPGEIVMSGGNEVFVKTLDGAV
                     SLEKVQAEGKNPLDIVSFMNGTGRNLFQKGRIFE"
     gene            24334..24894
                     /locus_tag="PLP02_03120"
     CDS             24334..24894
                     /locus_tag="PLP02_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPF42476.1"
                     /translation="MNRQKNLFSREEMYLMNVEKLHERGVSIEQIAEIAYKQQAKWNS
                     AISYNECLESVEKILSLRDVFHLLQLGAEIDRLTEAEAFNGPIQSILETDLGMFGIDE
                     LFGLELAGLYGTIGKTNFGDIDVNKPGIIGKLNDAGKKEKGHCHTFLDDIVGAIAAAA
                     STRVAQIENELEARKDPSIVEQPKLF"
     gene            24927..26531
                     /locus_tag="PLP02_03125"
     CDS             24927..26531
                     /locus_tag="PLP02_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42477.1"
                     /translation="MDKREKALDEFFRKNDNATKKKHVEKETKPENQAKIDEYMASVA
                     PVKSPKKETKLDKFLDFFRITSFKNRFDERKVSTNIKQYEIEGKGKLSIFFAKLKQFF
                     ISRFSFEAGIDILDETSVLYRKNMVIRNIIWLTNIVFMIFTFIGVTGVNLKINLIVIF
                     TIWIIMFFTSQSIRKIILQEPRTFHKQQLAEYVSGFYVFLMAMAVYIKLRVTLGTIDE
                     EAYFSITQAGYSLVYFALVVLSLYQDSKLLGTIFKVAIIAMTIVHVTILYPVYKYAGD
                     IVSLWNYIKGPVITDIILKTIVLAVFMVALYSIAKISEDLNAKRKEELVKRRAMEKDF
                     KAVVSDVFDVIGVYKQRVDENQEKINRKAAKRIADMASKLGNYLGYSPKLCKEIFDFS
                     TIHIDKISYLSVSDYDDKEVLDENDYRKIREKTIIGSVIIKRLQLEKKGEDIVRAHFE
                     KTADKDFIRDMNSIQDNRESQVILLCEIYDILRQPRNYKREMKHSRAIDLMQLEFYPY
                     FDPQIVDRFVKYGDEFESLYYKLCQE"
     gene            26544..27704
                     /gene="metK"
                     /locus_tag="PLP02_03130"
     CDS             26544..27704
                     /gene="metK"
                     /locus_tag="PLP02_03130"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPF42478.1"
                     /translation="MYRFFTSESVTEGHPDKICDQISDAILDAILADDPNGRVAVETL
                     VTTGLVLVAGEITTSTYVDIQKIVRETVAGIGYDRGKYGFDAENLAVLVAINSQSPDI
                     AIGVNESVSHEMGAGDQGMMFGYAIDGNDEYMPITHVYANRLSRRLAEVRKTGILHYL
                     RPDGKTQVTGEFDSEGNLVRFDKILVSTQHAPEITQDTIRKDMIKYVISEVIPENLID
                     KDTEILVNPTGRFVIGGPSGDSGLTGRKIIVDTYGGAVHHGGGAFSGKDPSKVDRSAA
                     YACRYVAKNVVAAGLAKEIEIQVSYAIGVAKPTSIGVNTFNTGKLPDEKLIEIIKKCF
                     DFRPRAIIDYFQLCRPIYKQISAYGHFGRTDLDLPWEKLDLIPTLKKLEKEG"
     gene            27707..28195
                     /locus_tag="PLP02_03135"
     CDS             27707..28195
                     /locus_tag="PLP02_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42479.1"
                     /translation="MMFLATIKQTIEIIIVITVPLAILVTGNVLMIRSLKTGYKDVYK
                     FQSKFDIELRKTINLFSKIDPEGILKDYRETVIKNISHEEKIELLDLVDEEYKLIDKE
                     SGNNEYAMETYENLQEMRRIHDTKALIFNHKILMFPFNVYARLFKIKKWDLYLHEKKE
                     ES"
     gene            complement(28238..28774)
                     /locus_tag="PLP02_03140"
     CDS             complement(28238..28774)
                     /locus_tag="PLP02_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF42480.1"
                     /translation="MITVAKWSHEWLLKDKLSGKTLVDMTAGKGQDTLFLASLSHDVY
                     AFDIQEEAIATTSELLRKNHVDWVKLINDDHAKIDEYIKVPIQGAIYNLGYLPGGKKK
                     IRTTAESTLTSLEKLLKMLSPGGIVVIVVYQKHEGNESQLLLRFVERLPGQTYDVSKH
                     SVLNKELAPYIIVINKAE"
     gene            complement(28771..28938)
                     /locus_tag="PLP02_03145"
     CDS             complement(28771..28938)
                     /locus_tag="PLP02_03145"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42481.1"
                     /translation="MELLRPDIIIYRLTGDSPRKELVAPLWSLKKFTVTNDIDKLLRK
                     RHSHQGARYEG"
BASE COUNT         8414 a         6667 c         6905 g         6962 t
ORIGIN      
        1 cattataaaa taggccaggt tttttgacaa gatgtatgat aaaatagtag gtgaatgaaa
       61 cgaggtatac gccatgaagg atttgttgat gataagatac ggtgatctga tcttaaaagg
      121 caggaaccgc aacagtttta tggacgcggt caaccggctg atcaaggaaa aactcgccgg
      181 tctttcggtc ggcttggaat tctgccacga ccgcgcctat gtgcatctga acgatgcgcc
      241 tttggacatt gtcataagac agctgggtta tgtgacaggt ctttattcat atagcctgat
      301 gtctaagtgc caggtagatt ttgatgaaat cgccgctttg gcgatagaac tgctcacgga
      361 ggacaccaaa ggacaagaaa tcactttcaa ggtggaaacg aaacgagtgg ataaaaccta
      421 tcccatgcaa agtcctgata tttccaaggt gttgtcggga atgatccttt ccgaaatgtc
      481 gaatctgatc gttgatgtcc ataatccgaa attgactctg acgatcgaaa tccgcaaaga
      541 aggagcctac cttttcaccg atcagatcaa gggactgggg ggatttccga ttccgatcgc
      601 cggcaaggcg gtggtgctac tatccggtgg catcgattct cccgtcgcct ctttcttagc
      661 gatgaaaaaa ggatttcagg tcgaacttgt ccattttgaa tcgacgccga tgacatcgat
      721 cgaatcagca cagaaagtca tcgatctggc ggaaatttta tcccgctatg cgcctcatga
      781 cgaaatccgg cttaattttg ttccctttac cgcgatccac gagaaaatca tcgcttcagt
      841 tccggaaagc tacattatta cgatcatgcg gaggatgatg gtcagaatcg catcgggaat
      901 caaggaaaaa accggatcgc tggcaatcat caccggagat tcgatcggcc aggtggcttc
      961 gcagacaccg gaaagtctgg cgacaatcca agaagcgatc aatgatctcg tgatcagacc
     1021 gctttcgacc tacgacaaga tggaaataat ggaggtagcg agaaaaatcg ggactctgga
     1081 gatatcaaac cggccgtttt cggattgttg caccgtatat attccgaaaa gcccggtgat
     1141 cagaccgtcg gtggcggcgg ccgtcaaata tgaggaaagc ttcgattatc gtccgatgat
     1201 cgaagaggcc gttaacaaaa ctaaaacggt cacgatcatc gcggacaaac atttggacat
     1261 ccagtctaaa ggactttcca taaaggacgt tctgtcatga aaactatcga gaccaaaaga
     1321 ctgatactgc gtacctgggc ccaggcagat gcttcggaca tgttcgatta cgccagtttg
     1381 gcaactgtcg gacctagtgc cggctggaag ccgcacgaaa cgatcgagga aagtcaaagg
     1441 ataatcgctg gcttcatcga aaaagatgac gtttgggcgg tcgaactgaa agaaaaccac
     1501 aaggtgatcg gatcggtggg aatgcacgaa aggcagacta tcggaggaat tatcaccaag
     1561 gaacttggtt atgtgctatc gacgccatac gaaggcaaag ggctgatgac ggaggcatgc
     1621 aaggcagtaa tccatcatgt tttccacgag actcaagtcg atgtgatcgc ggtctatcat
     1681 tttctggaaa acgacaaatc ccggcgcgtc atcgagaaat gcgggtttat ttacgagaat
     1741 atcatcgaat ataagacatc aacgggggac attcaaacct ccaaaagcta taaattgaca
     1801 aaggagagct atttgaaaat ggaagaatta agaaactaca cctggaatct tgacaaacta
     1861 tacacgtcat tcgattccaa aaaattcgca gatgacaatc tggctttgga aaatctggtt
     1921 gacaggatca aagcgaaaca ggcggatttt gccaactatg acgagaaagt gtccaaactg
     1981 aaaagctatc tcgaactcaa catcgaaatt accgtgcttg ccatcaaact catgaattac
     2041 gccgggttgc agggagctac cgattctacc gaccaacagg ccgccaaaag cgaaaaccgg
     2101 ctccatgtga aacttgccga aacggctgcc gtactcgccg cttccgccaa gtggatccgg
     2161 aattttcccg aacttgacga agctatcgaa gcggatcctt ttctgaaaga acacgaattc
     2221 atgttaaagg aaatcaggga aaacgcctcg catcttttag atgacaagac cgaagctctg
     2281 ttagcccagc tgaaaccgtc tggttcgacg gcatgggaaa gactccaggc tttattgact
     2341 tcgacggtcg ccgtcgacta cgaaggcaag gagattacgc tttcccaggt cagaaacctg
     2401 gcttatgaaa aagatcccaa agtcagggaa aacgcctttc gtgcggaact gaaagcctat
     2461 gaaaagatcg ataaaccggt ggcatttgcc atcaacgcaa tcaaaggtga agtcaatgtc
     2521 ctttccgcag cccacggcta cgaatcggct ttggcggaag ccttggcaaa gagccggatg
     2581 caaagagcga cccttgacgt gatgatggaa gccatggaag agtatcttcc gattttccgt
     2641 aaatatctga aacgcaaagg cgaattgtta ggacacaaga acggattgcc tttctatgat
     2701 attttcgctc ccatgggaaa aagcgagaag actttcacga tccccgaagc gcaggaatac
     2761 atcctgagaa atttccggac tttcaacgag cggctttaca atatggccaa acgggcgttt
     2821 gaagaaggtt ggatcgatta tttgccgaaa aaaggcaagg tcggcggcgc tttctgttcc
     2881 aacatccatc cgatcggcga aagccgggtt ttgtcgaatt tcaccggagt tttcggcgat
     2941 gtcatcaccc tggcgcacga actcggacac gcttatcacg gcgaatgcat tttcggcgaa
     3001 tccatcctca attccgaata tacgatgcca gtggcggaaa cagcttccac cttctgcgaa
     3061 accatcgtga acaaagcggc gctcaaggat gccgtatcca aagacgaaaa gatcaatctg
     3121 ttggaatcgt cgattcagga ttacacccag gtgatcgtcg acatcatgag ccgtttcatc
     3181 tttgaaaaga acctgttcga aggccgcaag aaaaccattt tcgacgagaa cgaactcaag
     3241 gaaatgatgc tggacgccca aagaaaaacc tatggagacg gactcgatcc cgaacttctc
     3301 catccctaca tgtggctttg caagagccat tactattccg gcaacctcag tttctacaat
     3361 ttcccgtatg ccttcggtct tctgttcgcc aagggacttt atgccgaata tctgaaagac
     3421 aaggaaaaat ttgtcatgat gtatgattcc ttattggccg cgaccggaaa gaacacggtg
     3481 gaagacgctg ccaagatcgc cggaatcgac atgaccaaaa aagagttctg ggtgtcttcg
     3541 ctgaaacttc tggaagaaga tatcgaagaa ttcatcagat tgacggaata atctcacaat
     3601 aaaccaacaa atgtttccgc ctctttgttt atattgatga atcgggaggt aagaacgata
     3661 tgaaaaaaat attgaccggc gccttgctcg tcctgctttt aatcggattg gccggctgcc
     3721 agaaaagcaa cggatgggaa gccgtcgatt tcgacaacgt cgaaggagtc ggacagtttg
     3781 aaagttacga tcagctgaag gattatcttg attcctatta cgaagaagac acattatatt
     3841 atcgaaccat ggattccaat tccgccgccc tggaaagcga tataatgaca actaccaccg
     3901 cacaaagcga tagtacggat gatgtcaact acagcgagac caacaaccag gtcaacggcg
     3961 tatccgaagc cgacagaatt ctgaccgacg gatattatat ctatgtgctg tcagacaacg
     4021 gattttatat tgtcgatgcc aacactttgg aattcgtcta tgtcttcaca aacgattacg
     4081 ggtacgctta cggactttac aagtacggtg attccgtggt ggtgcttatg cacgaatatt
     4141 actacatcaa ttacatgatc gaagatgaaa caaccacagt cacctccggt gcttcggatt
     4201 ccgatatcga tggatcatcc tatgtttacc tgaacaatta cggaaccaga gtcatcgtat
     4261 tcgatatttc cgacaaggaa aacgtgacgg tgacccgcaa tctgtttttc gagagaagct
     4321 atcttaacga cagccggatg atcgacgatg tctgttatct gatgatggaa aactatgccg
     4381 gcagatatgg atacgacgaa gacgattatt tccctgtttt ttatgacgat gctgcatccg
     4441 acaaagcgat tgatctgcca atcgaccata tctattacat gccgggaaac aattactata
     4501 tttcttacat gctcctggcg acattttccg tggataacga tgaacaggcc gatgtcaacg
     4561 cttatctcgg cagcagttcc gacctttaca tgagtcttga caatctttac gtggtctgct
     4621 acagatacag ctacacccaa gtatttttaa ccgatgcgag caacgatgaa acggtctttt
     4681 cgaactggta tttccaatac tggacatatg tcatccgttt cggagtggaa gacggcaaac
     4741 tggaatacga ggccgttgcc aaaatcaacg gatatccctt gaatcaattc tcaatggatg
     4801 aatatgacgg ttatttccga atcgccacca cgggatatgt gtatagcgaa gattactcca
     4861 catccacgat taccaaccaa ttggcgattt ttgacgccac caccgaaggc gagatcgaac
     4921 ccgtatcggt tttgaccgga ctcgggaaac cgaacgaaag gatctatgcc gtcagataca
     4981 gcggcaatat tgcttatgtc gtaactttcg tgaataccga tcctttgtac aaactcgatc
     5041 tgtctgatcc ggaaaatccg gaaatcgtcg acgagttgta tgaagaagga gtatcggatt
     5101 atcttcatga aatcggagac aatctgcttt taggcatcgg tcgtcaggct gtgaccgaaa
     5161 acggattcac gcatttcacc ggagtcaaag tcgctctata tgacgtgtcg ggagacgaaa
     5221 caatcaatct tgaaacttac tttgtcgaag gcgattacag ttattcgccg gtcacttacg
     5281 accataaggc ctttgtgagt tacgaaccgg aaggcgccga ttacatgtat gtgacgatcc
     5341 cggtctacga atattattcc gattggtacg caagtctcca gagtgtttat gttttcaaag
     5401 tttattacgc cggagatttg gaatttatca ccagattgac gcatatggac gacacgtccc
     5461 atacatactg gtaccattac gattcgatcg aaagaaccgt gatgatcgag aattatatct
     5521 atacggtttc ctataaccag atccgaaaat acgatatggc gtccggattc actctcgatg
     5581 tcactaatta tttccattcg gaagacggcc agtattaccc ttattacgga ttttacgact
     5641 gaataaaagc cactatagac aaaaacgcgg ttataaagcc gcgtttttat ttttcatcaa
     5701 atgaaaaatc tcgcgaaata tggatttttt tctttctttg tgatatattc attttataat
     5761 agataatatt ttaatttcaa aacaaaaaga atggaggaat gacaaaatga aacgaaggta
     5821 ttttgctttg ttgctgttcc tgttattcgg tatgttcatg gtcgcctgcg ggaacgatac
     5881 attgccgacg accaatttaa catccaatac cacctctgca acaactttga cgacaggatc
     5941 gaccacagtg tcgacgacag cgtcaaccac agcttccaca acgactaccg tttataccgg
     6001 tgaaacggtt acggttagat tccttgatgg gaatgatgtt ttgatttcag aagtcgttat
     6061 tcacaaagga gcgtcagtag taccaccgac gcctccgaca aaagcagcga caacttatta
     6121 cagttatgtg ttctccggat gggatcacgc tctgactaac gtcaacgagg acacaacaat
     6181 ccgcgcacag tttgtcgcaa cttatctgtt tacgaccggc acatttaatc atgcggatct
     6241 tttgactttg atcagaaact tgaccgatga caccgcatcg tccgatgcgg aaatcgaagc
     6301 caagatcgcc atgatcatga ctatgatcgg aatttcatcg gaagagcatc tttatgatct
     6361 gctttcggaa gttcccgatc tgatgacttc attccaggca ataaccagct cctccgaatt
     6421 cgccacctgg tatgcccagt taaaagcggc aggattcaac aaggaactca taaccgatac
     6481 cgcaatcaac ttgatcagaa acggcgtcgg tatgtggatc gagaattatg atccagagga
     6541 tttcgctgac gagattctcg gttatcagaa ccagatcagc gtcaacgaag ccgcaattac
     6601 ggatatccag acggcagtca acctgtattg cactctctat acgtccgatc cggccaattg
     6661 ccagttgctg ttctcttata ttttggacga tttctacaat tacatcaatt acgacaatat
     6721 gtattattcg tatttctatt ccgtcgattg ggaatacagc gacgaatatt ataatctcca
     6781 aaattatatg gatagctata tttatttctc gtatcaagaa ggaatggaaa cagaagccgc
     6841 caacgcgctt tccgaataca ataattacta tagttcgctg acggaatatc aacaaaacgc
     6901 ttttgatccg atcattgatg cctttagtac ttattccgaa cactattggg catacacgac
     6961 tccgcttacc aacgatttgt cgggactcac agatacgcaa tatggcgaaa atgtcgtaca
     7021 gtatctgtat agtcaggaac agagcatcga aaacctttat tggaccaatc attctctata
     7081 tcaagatatc gccaatgttc aggacgagat ggttaggatg gaagagcagt tcatgatgtt
     7141 ccagacattg cagcagtatc tgcagaccca ggacggactg gacaatctca agatattggt
     7201 gatcgctttc tatgattcgc ttgatgcgat cgcaaccgct ccgaacgccg atctgttcaa
     7261 tctgatcacg acccttattt cggaaggcga agaattcgat ccttcgacga ttaccacatc
     7321 cgacgcactg gcctatcttg tcggtctcgt cgacatgatg gagatcgtcc agaccaatat
     7381 gctttccacc gcttcgcttg ccaacatcat tgaaattgcc gaagacctca tgccgcaatt
     7441 catcaacaac atggatgatt tgacatcggt tgaaaaggcg gcccttatcg ccaccatcac
     7501 tccgaagatc acccaatatg tcaacatgtt cgccagcgaa tacgacaacg ttctcacttt
     7561 ccttgacagc atgaatttcg tcaaaggaga agccgtaatg gatttcgtcg cgttcatgag
     7621 cgaatatgac gaacagaatg atacccaaat cggcggagaa tatcccgttc atatgattta
     7681 tcaggtatcc gttttggtca atacgcttat cggcgacgat tcatttgatg tgtcgcaact
     7741 gttaagcgat ctgatcgtcg tttactatga catcgatacc gaattccagg ctgtgccgtc
     7801 tgaagtgacc gatacccagg ctgccgccac cgccaatgtc gaaaggatca tcgttcttgc
     7861 cggtatcatc gcggcgggag atcccgataa tctgagcccg acccaaatgg cttcttatgt
     7921 ggaaatcggc atccgggtcc aagctttcat caccatgttc agcgacggat tcgaaaccgc
     7981 tcttgaaccg atcgtcacga cttatgtcca cggcgctttg gttgaggcca tcacccatat
     8041 gttcgacgta tccgaaaccg aagccgaagg catcatcacc gacatgcttg tgatcatggg
     8101 cttcgaagac gaagaagaca tgtattactt ctttgtatcg gttgccaccc atgcccagga
     8161 gatggaaatc ggcatcaata actatacaga tctggaaagt tggatcaact ggttctatga
     8221 catgggctat accaaggaag aaatcgccga catcgtctat gagatggctg tgtatgttat
     8281 cgatctgaga atcgccaccg gcggaatatt ggaaaatgac attgcctatt acgaaaacca
     8341 gattcttgtt tatgagggat tgatcgtgga cgcaaacgcc gccatcgatt ccctgtccgg
     8401 cgaatttacc ggcatgctgg gaatggcgag tccggaagag gttgatctgt tcatgaactt
     8461 ctggaccctg cagaaagaaa aatcgatgcg tgaatacacg atcatgtaca tggaatacga
     8521 cgtggattac gaatacttcg attggggaac ctatgacggt ctgaaaacgt atctgtatga
     8581 agattatata ggaactccgg gagctatgga tagttacaat tccatgttct attcgctttc
     8641 accggatgag caggccgcat atcagcctct aatggatcaa tggatggcat attatgattt
     8701 cctgccgacc tatgaaagca gtcagtccga agtatctagc accagcaacc tgacagatgc
     8761 ttacatcttt gtgatgaaca acctgcaata cagttacgaa aatcagctta gcaacatctt
     8821 cagctatgag agccagatca catggatgac ggaaaagatc gatgatctga acggccaaat
     8881 ggaacttctg acggttctgt ccgagtatct cgccaacacg gcggacggca aagacgaact
     8941 cgtcaagaac gtcatcatga tcgcgcttga tgaagtcgag aatcttctct cggcgaccga
     9001 cgacgcgacg atcgatatgt tcatcgcttt gattcagaat ctttccggca tgccggataa
     9061 cggattcgat ccggcaacca tatccgcttc ggacatttac aattataccc aggaagcagc
     9121 caatatcctg acaaccctcc tggcaacaat cgattcaacc gatcaggcca acatcagggc
     9181 tttcgttctt gatgctctcg agatcaagtg gactgccgaa ggagaaatga cgccgacgga
     9241 gatcagcgat cagctaacga tcatcggatc gacattcgac aagtattatc cgcgcctcgt
     9301 caccatcctc ggaatgcttt ccaccgcgct tatccagctg gataccacgg aaatcggaga
     9361 tatcatggac acggccggca atatcgcggg ttatgacaat tcgatcgcaa tgatgattgt
     9421 ctacgtatcc gaaatcgccg acatcttcct gacagacact ccgaccgctc ttgattacac
     9481 caccctggtc gattacgctg tcgacgcata cttcgatatc acatacgatt tcaactacac
     9541 gggagaagta aacatcgccg atttgcagga tgtctatgaa caatacgccg cttcgatcgc
     9601 tgcttacttc catctgatcg cagccatgga tccggacaac ctgacaaccg ataacgtcaa
     9661 actcatcttc tcttcgtatc tgtatgtacg gggcttggtt ggcgaaatcg aagaaggcgg
     9721 attcgaaacc atgacttata cggcacccga catgatgtac ccgtattaca cctacgatta
     9781 tcaggatttc tacgatctcg tggatgatat gatcggcgga gacatgacgg aaaccgagat
     9841 caacgattgg atctccgatt atgtgacaga ttccggactt accgcggaag aagccatgta
     9901 tcaactcttc atgaatggtt taggagaacc gacggaataa tcaaatcact ctgtttcaca
     9961 gggagccaaa ttgctttggc tccctttttc ttttgtgata taattaggat cgggagtgat
    10021 accattgatc ataacgtccg ccgataacca aaagttccgc tcttatctta gattgaaaca
    10081 gaaaaaatac cggtaccaaa ccggtctgtt tttggccgaa ggaccgcatc ttgtgaatga
    10141 agccgccaaa gcaaggcgtt tggaagcggt cgtttctacc gagccattca ccgatatcaa
    10201 agtggataat ctggttgttt ccgcctccct tttcgcatcg ctttccgatg tcgagacacc
    10261 gcagggagtg atcggcatct gcaaaatggc agacaatgtt ttgcttggcg accggatttt
    10321 gatgctcgac ggcatctccg atcccggaaa cctgggaacc ctgatcagaa gcgcaaaagc
    10381 cttcggattc aagacattcg tctttgaaaa cattgccgac ccttattcac ccaaagtggt
    10441 gagagcgacc cagggagcga ttttcggact cggatttttg aaagccgatc tgatcgaatt
    10501 catcggcaaa caccgggaat acgaatatgt cggcaccgat gtgaaaaccg ggttgcccct
    10561 ttccgaattc aatccgaacg gaggcaaatt ggccttgatt ctcggaaacg aagcaaatgg
    10621 ggtcagagaa gaaatcttga ataagacggg tgtgaacata aaaattgaaa tggacggcat
    10681 ggagtcactg aatgtcggtg ttgccggcgg tattttgatg tatgccctta ggagaaaatg
    10741 aatatggaat ttcgatatga agaagagaaa atatatttgc tggacgaatc aaggaaaacg
    10801 atcggagaga tcgacatccc gaagcgcgat gaaaagcgga tcgacatcac ccatgtgttc
    10861 gtcgacgaat ctttgcgggg acaaggctat gcccaagccc tcatgcaaac ggcttacgat
    10921 tacatcaaaa agcaaggaaa actgatcgtt gccaagtgcc cttatgcgat tgcctggttc
    10981 aaaaaacact acgaatgcca ggacatcgtt ataaacgtca aaaccggccc gaaagtgtga
    11041 ttttcgcttg cctgccgtat catcttgtga tataatgttt taggcataag gtagaaatgg
    11101 gagggtcgga ccctctcatt tttaataaaa acggaggcat gaatatggat ctcgctttgt
    11161 tgaagaagca agtggggcag gaaattgaga aatacggtta cgaactgtac gacttgatat
    11221 accacaagga aaagaaacaa aacgtcctga caatcatgat cgacaaaccg gaaggcatct
    11281 cgatcgacga ttgtgtcaat gtgtctcacc ggttgtcgga ttttctggac gggatcgatc
    11341 cgattcagga tgcctacagc ctggaagtat cgtctccggg agcggaacgt gaactccgta
    11401 acgacgatga aatcgaaaaa gcaatcggca aatacatcca tatcgaaaca tacgaacaaa
    11461 aagccgacgg agaactgatc gcttatgacg gagacgtcat cacgattcgt ggagaaaaaa
    11521 acaaacaaat gtcatttggc cgtctggatg tcaggacgat cagactggca atcaaattct
    11581 aggagaagaa tattatggtc agcaaagagt ttttcaaagc attggaacaa gtcgccgaag
    11641 agcgcgatat tcccaaagaa aagattctgg atatctttgg aaaaggactc ctgaacgctt
    11701 acaaaaaaga ctataacgga caacagaacg ccaaggtcgt attcaacgag gaaaaggcag
    11761 aaatcatcat cgtcgctact tatctcgttg tcgaggaagt cgatccagaa gccgagaaag
    11821 gcacccagat caccgttatg gaagcccaac aactgaaaaa gaacgccaaa gccggagata
    11881 taatcgaagt taaagtaacc ccgaaagatt tcggtcgcat tgccgtatcc agcgccaaac
    11941 agattcttac ccaaggactc aaacagcttg aaagggaacg caacttcgag atattcaccg
    12001 ataagaccgg cgaaatggtg attgccgaaa tcgtcgccgt caacgacgaa ttcgtcactc
    12061 tgtatctcgg attcgatacg gagacatcga tccccgcccg tgatcttgtc ggaacagaaa
    12121 tattcgtcgg taatcagatc cgtgtctata tcaccaaagt cgaacagacg accaaaggcc
    12181 cgaaagtcta cgtctcacgt actgacagaa acctggtcaa acgtttgatg gaacagtcga
    12241 ttccggaact ccaggaaggc gtcatcgaga tcatgggtct cgcccgcgat cccggagacc
    12301 gttgcaaagt ctgcgtcgct tccaacaact tgcgcgtcga cccggtggga gcgtgcctag
    12361 gacgcaacgg agcccgtatc cacgaagtca tcgacgccct tgacggagaa aagatcgatg
    12421 tctacaaata ctcagaagat ccggcggaac tcgtatctaa cgccattcaa cccgccaaga
    12481 cgacctcggt catcgtcgat cagcgggaaa agagcgccgt catcatcgtt ccggacgatc
    12541 aattgtcgct cgctattggt aagaaaggcc aaaacgccag acttgccgtc cagtcgagcg
    12601 gttggaagat tgatatcaaa tccgaaaccg acgccatgat ccaaggaata aaatactgac
    12661 cggaaggaag atgttccgat ggcaactatt aaaaaaattc cgctgcgccg ctgcgtggtc
    12721 accggcgaaa gatgtgaaaa gaaaaatctg tttcgcgtcg tcaggacgcc ggagggacaa
    12781 gtgatctatg atcccaccgg caaagccaac ggcagaggag cttatgtttc cagaaataaa
    12841 gccgtgatcg aaaaggcccg gaaatcaaaa gtgctggcca agcatctgga atgtgagatt
    12901 cctgatgatc tttatgacct gcttcttggc aggttgactt cgaatgagta aggaacaagt
    12961 cctgaaaaca ctcggtttgg cataccgggc aaaaaaggtg tcatccggcg aggaagccgt
    13021 tttggaccag ttgaagagag gttcttcctt tctcgtgctt ttggcaagcg atgccggagg
    13081 tgcgacaaag aagcgattca ttgacaaaac gaaaagccat gccgtgcctt tgataagcaa
    13141 ctatactgcg gaagaactgt cccatgccat cggcaaggag ggccgcaagg tcctgtcggt
    13201 gtcggatagt aatttcgcca gactcctttt ggaaaaacag gagtccgaat aacaagaaag
    13261 aggtggtatt atggcaagcg ccaaaaaacc catgcccaag aaaaaatccg acttcaaggg
    13321 aggcaagtcc aaccagaggc cggctaatcg tcagacgcaa aataaccgcc agacgcagaa
    13381 ctatcaaaag ccaaaattgg agaaacagcc ggaaattctc gaaaagatcg tccatttcaa
    13441 gaacgggatg accgttttgg agctttccga acaactgcat aaatccgttg ccgatgtcat
    13501 caagaaactg atgttcatgc ggatcatggc ttcccagacc caatcggtgg atcgcgatac
    13561 cgccgaactt ttggccatgg aatacggctt tgaaatccag gacgaagtcc tgaccgacat
    13621 gacacgtttc gacgaacttg cgatcgaaga cgatccgaag gatctggtcg aaagaccgcc
    13681 ggtagtgact atcatgggtc atgtcgacca tggcaaaact acattgttag acaccatcag
    13741 gcattcgcgc gtcgtccaaa gcgaagccgg aggcatcacc cagcatatcg gtgcctacca
    13801 ggttacgcgt aatggcaagg aaatcacctt tatcgatacg ccgggacatg ccgcattcac
    13861 ggaaatgcgc gcccgcggcg ccaggatcac cgacatcgtc gtattggtcg tggcggccga
    13921 tgacggcgtc atgccgcaga caaaagaagc gatcgaccat gccagggcat ccaaatgcgc
    13981 gatcattgtc gccgtgaaca aaatggacaa accgggcgcc aaccaggacc gcgtgaaaca
    14041 ggagttggcc gattacggtc tgttatctga cgactggggc ggcgaaacgc ctttctgtta
    14101 tatatccgcc ctcaaaaata cgggagtgga cgaacttctc gacacaatcc agctcgtatc
    14161 cgaaatgctt gaactgaaag ccaatccgaa acgcaatgcc gtcggaaccg tcatcgaagc
    14221 aaaactcgac aaaggccggg gaccggtggc caccattttg gtatcgaacg gcaccctgaa
    14281 gatcggcgat tcattcgctg tcggtacgac atacggaaaa atccgggcga tgaacgatga
    14341 tttgggacac acgatcagaa aagccaaacc gggggcagcc gtcgagatca acggactgaa
    14401 cgatgttccg caagccggcg atgccttcat ggttttcgat gatgagaaaa tgacgagaga
    14461 tatcgctcaa aagcgtgaag agcgtcagtt taaggaagaa cacaatacga aaaaaacgat
    14521 atccctggag gaattcttca ataaggccga aggagtcacc aaggaactga gactcgtaat
    14581 aaaggccgat actcagggat cgatcgaagc attcaagggt tcgatcgaga aactgaatat
    14641 cgaaggcacg acggtcgata tcgtccgtac cggcgtcggc gccatcaccg acaccgatgt
    14701 gtcgctagct gaagccagcg acgccttcat catcggcttc aacgtccagg ctcccgccaa
    14761 tgtccgcgat accgcagggc aaaaaggcgt cgaaatccgc gattaccgcg tgatctacga
    14821 agcgatgaac gatatcgaag cggccatgaa aggtttgttg gaaccgaaga tggaaagact
    14881 cgtcatcggc gaagccgaag tcagggaaac cttctccatc tccaaaatcg gcaccatcgc
    14941 cggttgcatg gttacatcag gtgtgattcg ccgagacagc caggtaaaac ttttccggga
    15001 cggtaaagtc atattcgaag gtccgatcgc atccttaaag cgtttcaagg acgatgccaa
    15061 ggaagtcaaa caaggctacg actgcggtat tatgatcgaa aactacaacg atgtcaaagt
    15121 cggagacact atcgaagcat ccgttttgaa agaggtagct tgaaatggca aagaccgaca
    15181 gacttgacac gttggtccaa cgggtttgcg ccgacatcat caaaaatgaa gtgaaagacg
    15241 acttaggctt catcaccgtg accggagcca gggtgacgaa cgatctgtca tacatgtatg
    15301 tttattacac aatctacggc gacaatgaac gccgggaaaa aacgcaggca gccttggaaa
    15361 gggctaaagg cttcatcaga ggtcaaatcg ccatgcaggt caaaatgcgc aaagtgccgg
    15421 aactgatttt caaatacgac caaagcgact tagaaggccg caaagtcgac gaaatcatcc
    15481 gcaggatgaa atgaggtata cccgggcgga aaccgtccgg gttttccata ttgtttgggg
    15541 tgtggaaatt gaacgagaaa atcaaggcga aactcgccct tctgcccgat gccccgggca
    15601 gttatcaaat gttagacgat gcaggtagga tcatctatgt cggaaaagcc aaaagcctga
    15661 aaaatcgggt aaaaagctat ttcagcggca gccatgacgc caagacgacc aatctggtcg
    15721 ccaacatcgc cgatttcact tatattgtga cttcttccga actggaggct tttttgctcg
    15781 agctctcgct catcaaggaa catgcccccc gcttcaacat tctcctgatg gatgacaaaa
    15841 cctaccccta tatcgagatc accagggaaa agaatcccaa actggtgatc acaaggaagg
    15901 caaacaccaa aaaccggttg cttttcggtc cttatcccga tgcttcgtca gcccgggaga
    15961 ccctgtcgtt aatcgaaaag ctgtttccct tccgcaaatg caaggcgttg ccgaaaaagg
    16021 tatgtcttta ctatcatctc ggtcaatgtc tggggccgtg cgaatatcct gtcagcatag
    16081 atgtctacaa gaagatggaa aatgatgtcg ccgcttttct ttccggccgc gacgaccagg
    16141 tgctagacga tctggaaaag aagatggaag actgttcctt gaacaaggaa tacgagaagg
    16201 ccggggaata tcgcgatctg atcgcttccg tcaaacggac gctcagaaag cagaatgtta
    16261 ttttcaacga tctgtctgac cgcgatatca tcaactacgg gttttacgac cattatctcg
    16321 ccgtatcgat tctcttcatg cgccagggga agattatctt ttccgaaaac aaattggtga
    16381 cattctatgg cgaaccggaa gaagcgttca tcagttatgt ggcccagttt tacgaattgc
    16441 atccgcttcc aaaggaaatc ctgttgccag aaggtccgga ttatgaactg cttgagccta
    16501 ttttgaatga cctcggtttc gttccgaagc gcggaaaaaa gaaagaactt gtggatatgg
    16561 cgaaggaaaa cgccggtatt tatctagaaa ataatctcag tccctatctg agaaagagcg
    16621 atcaatcgat cggtgcgatt caaaccttgg cggatatgct aggcattcag gttccggtaa
    16681 ggatcgaagc gttcgacaat tccaacacga tgggaagcga tccggtgtcc tcgatggtcg
    16741 tgtttacgaa cggactgcca gatcgcaagg aataccggaa atacaaggtc aaaaccgttg
    16801 tcggacccga tgattacaat acgatgaagg aaatcgttta tcgacgttac caaagaatgc
    16861 tcgtcgaaaa atcgagacgt cccgatttga ttgtcatgga cggcggacta ggccaggtga
    16921 acgcagcttg cgaaatcatc gcttctctgg gattagacat cccggtggcg ggcctcaaga
    16981 aagacgaccg gcaccatacc gatttcctaa tatcgccaaa tggcgaagaa ataaaactcg
    17041 accgacacca accgatctat gttctgctga acaaaatcca agaagaggcc cataggtttg
    17101 cagtgacatt ccatcgtgac agacagtcaa agcaggtcta tgcctcttat cttgacaaga
    17161 taccatccgt tggcaaggcg accaaggaaa agttgttaca aaaatacaag acattggcta
    17221 atattgccaa gtcggacgac caggaactct cggagatcgg acttacgaaa gtccagatta
    17281 aaaatctgag aattgccctt gccgatctgt caagccaata aaaaacgtcc ggaacggaca
    17341 aattgtggta aaataatttt gatgatcgtt ttaaactgat gaggataaag atgacagagt
    17401 acgtatacgg acttttacac ggagtcgatg aaacaaaaag gattatagcc atcaagattg
    17461 ataataagat ccggtttttt taccttgcca aaggtcaatt tactgttttc atggcttatt
    17521 tcaaaccgaa tatttatgtg ttcatgaccg tggccgaaac accgaggcat tatcgcggat
    17581 acaaagttca gaatgttatc aacatcgaaa aagtgatgtt gccaaacaag cagaaaccga
    17641 cggtctttta cgacatttcc atcatccgtt ccgggatcaa gacgatcgtc gattcgggga
    17701 agccgaagct ttttgttgat ttcgagatgt ccatgccccc ttaccgggat taccggactt
    17761 tcgtttccga gattatccag gccggactgg ttctgtgcga cggcgacgga aaagtgttgg
    17821 aaaaccattc tttcttcatc aaaccgcagt tgtttccgaa aatcagcgaa cggactggca
    17881 aatttctgca tatcaggcaa gaagacgtcg atagcggccg ggaatacggg gaattccatt
    17941 ccctcttcat gaaaatccag gcttcctatc atccgatggt gttcgtttgg ggaaagaacg
    18001 acaagataga actgaaaaaa gccaacgaac gtctcggctt aacggaattt acccaaagag
    18061 cccagttcat cgacttgtta caacttcaca agatctattt caacctgaaa aacgatctcg
    18121 gactcttcaa cgcctacaat ctttactccg accacgattt ggacaaacag gcacacgacg
    18181 ccttcgaaga tgccagcgtg accaaggacg tgttttactg gtttaaggat accgtcaacg
    18241 gaaaccggga gattaccatt ccggaaaacg gcaaaccagc cgaataaaaa aacagaccgt
    18301 ttccataagg aagcggtctt tcttttataa aagcattaca accggaagcg tcatcggttc
    18361 ccgtccggtt ttcaactgga cataattcat gatcgctttt tgtagatcgg acttgatcga
    18421 tgcgacattc gctttcgttt cggccttaag ggtattggaa accgttttag ccgagatttc
    18481 ctggagttcg cgaagcatct gttggttgtc cttcatgaaa atgaatcctt tggtcacaat
    18541 tgagggcttg cagatgattt cgttggattt gctatcaatt gtcacgataa ccgacaaaag
    18601 cccgtcatcg gacaattctc ggcgatcctt gatgatctgg ctgccgactt cgccgattgc
    18661 cattccgtcc acatacacgt cggatgtcgg gattttgccg gcggcgattc tcgctcccgt
    18721 tttggaaagg gctagaatgt ctccgttatc catgacaaag gtattttccg gcttgactcc
    18781 ggcttcgact gcaagatggg cgtgagtttt tagcatccgg tattcgccgt ggatcggcat
    18841 gaagaatttc ggcttcatga gttttaacat cagttttaat tcgttttggc cgccatgccc
    18901 ggaggcatgg acgtcggcaa acggcgactg ggtgatcacc gaagctccgt ttttgtatag
    18961 aaggttgatg atttggttga tgctttcctg attgccggga atggcggaag aagataaaat
    19021 aacggtgtcc ccgggaatga tggaaatctg tttgtgcgtt ccggaggcga tcctggtcaa
    19081 ggcagccatc ggttctccct ggctgccggt acaaaggatc gtgatgttct tttccctgag
    19141 tccggacatg ttgcggctga agaggaaggt tccgtgcgga gcacggatgt atccgagtcg
    19201 cagtccgacc tcgatgtttt tttccattga gcgtccgaag acggcgatcc gtctgtttgt
    19261 cagggaagaa gcctcgacga tttgctggat cctgtgaaca ttggatgcga aagtagcgat
    19321 gatgacccga cctttgatcg aggcgaacag ttccttgatg ttttcggcga cgacacgttc
    19381 ggatttggaa aagtcctcaa gctcggcatt ggtggagtca cttaacaggc ataaaactcc
    19441 gtcttcgcct attttggcca ttttttggta attggcgtcg gcacttatcg gcgaaaaatc
    19501 aaacttaaag tcccctgtat ggactatggc gccttccgga gtgttcacgc ggatgccgaa
    19561 agaatccgga atcgaatgat tggtccggaa gaaactgact gagagattgt tgaatcggat
    19621 gatgtcggat tcatggtatt cctggagatt gatggtgagg cccgggtgtt cttccatttt
    19681 ggccttgatg aggccgatcg acaatccggc tgcgtataca gccggtatcc tgaccatttt
    19741 caaaagaaaa gggatggcgc cgatatggtc ttcgtgcccg tgactgatga agagagcctt
    19801 gatctttttt tggttctcga tcagataagt gtactcagga atcacgtaat cgatgcccat
    19861 caggaattcg tcgggaaata aaagcccaga gtccacgatg acaagttcgt cgcggtattc
    19921 cacgcagtac atgttcttgc ccacttcacc caatccgccg agggcaaaaa caccgacctc
    19981 gttgttactg aagaattggt ttggcatttt gaaccttttt cctttcaata ttactgttgc
    20041 tttctgaaat attatacata atagaagaag attttgcaaa caaaagcgcg agaattcttt
    20101 atcctttgcc ttgattttaa acactttggg cattaaaata aaaactaacc ggacaaaaaa
    20161 cagtcaaaac agaaactaat gtccgaaaaa ggagtttgca aaagatgcca atattgacta
    20221 ttttcctcat tgttctcgta atagtgatta ttttcagttt agccgccgta atagtcgcca
    20281 tcatcattgc atcgaaaaac cgcaaaaaac ggaacatcat acaaaacaat ctcgaaaaag
    20341 tgtatattga aaacattgtc gaggacaaag atgattgcaa gcttacaatc agggatttgt
    20401 caggaaaaag ggaaacgtat ccggttggca agaaagccat gcaatcgctt tatcccggcg
    20461 aaacgtgcct gatagcccgc caaaacggga aattgctttt atggaaagct caaacccagg
    20521 aaaaaccgga tttcttttca aaagaaaaaa acgataccaa tgtcatgaaa gtaacggcgg
    20581 aatcgccggt tttcgatctg gcgatatcca gcaactccgg tatcacggct tcacaagatg
    20641 aagtcttggg ttacatggac aggatgctgg aaaaccaaac cgaacatttc atcggtctga
    20701 aaagcgacga cggccgttat ctggaattat ccggaaacgg taaagacagg tatctggaaa
    20761 tcagactgat aaaaggcaac aggagttacc ggatgaacaa gtgtcttcta agtgtcgcca
    20821 aatccctggt cagggatttt tacggcaaag cggaactaac ggacaatccg ggatttgtca
    20881 tagaggaata aggcgcctta tggcgctttt ctattgtttg agattatgat ataatttatt
    20941 tgtggacaaa ggaggtgggg ctgtgattgc acgggtattg gtcgatgtgc cggccaaaag
    21001 cgtcgataaa ctttttgatt acctgattcc ggcacaaatt gcgggaatca ttgaaaccgg
    21061 aatgcgggtc atcgtcccct tcaataaccg caaactgatg ggatattgtc tggaaatcgc
    21121 cgaatcgagc gattatgaca aagatctcaa agaaatcgac agcctggtcg atctggaatc
    21181 atatctgacg gacgaactga tcgaacttgc caagaagatc agaaacgaaa ccgccacggt
    21241 tttgatcaga atattggaaa ccatgttgcc atcggccatg cggatgacat acactaccaa
    21301 gctcagagtg aaagacaggg acagactgcc tgacaaactt gtgccggttt ttgcctccca
    21361 ggacgagata atcctgaatg cttcacaaag ggaatgttat ccggaattga agaatgcgat
    21421 aaaagaaggc gtcatcactt cgacctttgt cgtcaaatca aaggggtctt tccggatgga
    21481 aaaagtcgcc atgctcttgc agaagaacgc cgtgacgaag tcagaaaagc agaatgcggt
    21541 cattgcattc ttacaagaaa aggaaaacat gaccgccaat gccgctttaa tgataaaaaa
    21601 actgaacgta acgatgtccg ttgtcgaaag tttggccaaa aaagggatca ttttgcttga
    21661 ataccgggaa aaataccgcg acttgttttc tctgatgcct gtcaacgata aaaccgtgat
    21721 tcttaataaa gaccaagaag tcgtgatgga aaagatcagg tcaaaatatg acagttttca
    21781 ggttcttttg ttacacggag tgacatcttc cggtaaaacg gagatttatc tgaaagcgat
    21841 cggagaaacg atcgcaaaag gaaaagaagt tatctttctg gttccggaaa tatcccttac
    21901 tccgatgatg gtttctcgtt tcaaggggaa gttcggggaa aaagtcgcca ttttgcattc
    21961 cggtttatcg ctcggagaaa aattcgacga atggcggaaa atcgtgcgcg gtgaagtcaa
    22021 gatagtcatc ggcgcgagaa gcgcgtgctt cgctcctttc agaaatatag gcctattggt
    22081 cgtggacgaa tgccacgagt catcctataa acaggacgac atcccgaaat attatgccat
    22141 cgacattctg gaaaaacggg ctcaaacata caatattccc cttcttttgg gatcggctac
    22201 gccgaacatc gattcttatg cccggttcaa acggggttat tacgaactat tggagttaaa
    22261 acaccgtgcc atgaattcca gtttgcccga tgtaacgaca gtcgatatga aacaggagtt
    22321 taaaaacgga aactccggcc aactttccga actgttgatt tcggagattg ccagacggct
    22381 ggaaaaaggc gagcagacga ttctcctttt gaaccgacgc gggttttcga cttttgtcat
    22441 ctgccggaat tgcggccacg tgttcacttg tcctgaatgc gacatctcgc taacttacca
    22501 tgaggcagac cattctttga aatgccatta ttgcaaccac aaggagccga taccgaaagt
    22561 ttgtcctgac tgcaagtcgg aagatttgcg gtatttcggt tctggtactc agaaaatcga
    22621 agcggaagtc caggaaaaat tcccgaatgc gagaattgtc cgtatggaca acgatacgac
    22681 cagaacgaag aacgcccatg aaaaactgtt atacacgttc gaaaagaaag gcgacatcct
    22741 tctcggtacc cagatgatag ccaaaggatt ggattttccc agagtgaccc tggtcggaat
    22801 catccaggcg gacgggaatc tttaccatcc cgatttccgg gctccggaaa agactttcca
    22861 gcttatcgtc caggttgccg gccgtgccgg cagacgcgat gaacccggaa aagtgatcat
    22921 tcaggctttc aatcccgatc attatgcgat cagatatgcc cgcaatcagg attacgacgg
    22981 gttttacaac cacgagatgc aattgcgccg gatcgccaaa tactcaccgt tctattttct
    23041 ggcggagatc aaattctccg ggcccaagat gcgcgatctg tttataagcg cccaagccgt
    23101 cgtcaagttg cttcggtctg aactggcgga agacaccgtc gtattgggac cggcattgcc
    23161 gccaattcct aagatcagga atcagtacag ttgccaaatc ctcgtgaaat accgtcatga
    23221 ggaacatatg aacgatttgc ttaacaggat acgtcatgat tttgaaaacg atcttgtcag
    23281 gattgcgatt gaccgcaatc cggctttggg atagaggtga caagcatgaa aatagtcttc
    23341 atgggaacga gcgaattcgc cgtcccgatt ctggaaggtc tcgtcaaaga atatgagata
    23401 gtcctggttg tcacgcaacc ggaccgtcct ttcggacgta aaaaaacact gaaaccgtct
    23461 ccggtcaagg aagttgcctt gcttcacaac attcccgttt tccaaccgga gagaatcaag
    23521 gaagatttcc aaccgatcct ggcttatgga gcggatcttt ttgtcgtcgc cgcctacgga
    23581 cagatgattc ccgaatcggt gctttattat ccgaaataca aatcgatcaa tgcgcacggg
    23641 tcgcttttgc ccaagtacag aggcggcgcc ccgatgcaaa gggcgatcat gaacggcgac
    23701 gagaaaaccg gcgttacgat catatacatg acaaagaaaa tggatgcggg agcgatgctt
    23761 gccaaaatag aagttgcgat cactatggaa gacgatctcg gttctctgga gcaaaaattg
    23821 ggtttagcag cccaaaaact gatgcttgag acgattcctt gtttggcttc aggaaatgtt
    23881 gttcccaccg aacaggattt gtcggaagtc acattcgccg ggattatccg tccggaagaa
    23941 gaaaaactcg atttcggacg aaaatcgaag gaaatctttg atcatgtccg ggcattccgt
    24001 ccgaagccgt tgtgccatgc caccatagac ggaaagaaaa tcaaaatcat cgaagtgaaa
    24061 atcgcaaacg acataaaagt cgaaggcgta cccggtgaaa ttgtcatgtc aggcgggaac
    24121 gaagtgtttg taaaaaccct ggatggagcg gtttctttgg aaaaagtgca ggcggaaggc
    24181 aagaatccgc tcgatatcgt ttcattcatg aacggaacgg gacgcaatct tttccaaaag
    24241 ggccggattt tcgaatagga atcgctatac atatagtttt cataaaaata tgatataata
    24301 atatgttagt ggataattga caggggtgat cttatgaacc gtcagaagaa tttgttttcc
    24361 cgggaagaaa tgtacctgat gaatgtcgaa aagctgcatg aacgcggagt aagcatcgag
    24421 cagatagcag aaatagccta caagcaacaa gcaaaatgga attcagcgat ttcttacaac
    24481 gaatgcctgg aatcggtcga aaagattctg tctttgcgcg atgtcttcca tttgttgcag
    24541 ctgggggcgg aaatcgacag actgaccgaa gcggaagctt tcaatggccc gatccagtcg
    24601 attttggaaa ccgatctcgg catgttcgga atcgacgaat tgttcggtct tgaactggca
    24661 ggtctttacg gaaccatcgg caagactaat ttcggcgata tcgacgtcaa caagccagga
    24721 atcatcggca agttgaatga tgccggaaaa aaggaaaaag ggcattgcca cactttccta
    24781 gacgacatcg tcggcgccat cgcagcggcg gcatcgacca gggtggcgca gatcgagaac
    24841 gaacttgaag ccagaaagga tccgtcgatc gtcgagcagc ccaaactgtt ttaagcaagg
    24901 acaaaatgaa aaaggggggt gacgcgatgg acaagcggga aaaagccctg gacgagtttt
    24961 tccgaaaaaa cgacaacgca acgaaaaaga aacatgtcga aaaggaaacc aaaccggaaa
    25021 atcaggcgaa aatcgacgaa tacatggctt ccgtcgcacc ggtgaagtct cccaaaaaag
    25081 aaaccaaact agacaagttt cttgattttt ttcgcatcac atcgttcaaa aaccggtttg
    25141 atgagcgtaa agtcagcaca aacataaaac aatacgaaat cgaaggcaaa ggaaaattga
    25201 gtattttctt cgccaaattg aaacagtttt tcatcagccg tttcagtttc gaagccggta
    25261 tcgacatctt agatgagact tcggtccttt atcgtaaaaa tatggtcatc cgcaacatca
    25321 tttggctgac caatatcgtg ttcatgattt tcacttttat cggcgtgacc ggtgtcaatc
    25381 tgaagatcaa cctgatcgta atcttcacga tctggataat catgtttttc accagccagt
    25441 cgatccgcaa gatcatcttg caggaaccaa gaactttcca taagcagcaa ctggcggaat
    25501 atgtatcggg attctatgtc tttctgatgg cgatggcggt atatatcaaa ctgcgcgtca
    25561 ccttgggaac gattgacgaa gaagcatatt tcagcatcac gcaggccgga tattccttgg
    25621 tgtatttcgc tttggtagtt ttgtcgcttt atcaggattc caagcttctc ggtacgattt
    25681 tcaaagttgc gattatcgcc atgacgattg tacatgtgac gattctctac ccggtataca
    25741 aatatgccgg agacatcgta tcgctatgga actatatcaa aggacctgta atcacagaca
    25801 tcatcctgaa aactatcgtt cttgccgttt tcatggtcgc cttgtattcc attgccaaga
    25861 tatcggaaga tctgaatgcc aaacgaaaag aagagctggt caaacgcagg gcgatggaga
    25921 aagatttcaa agccgttgtt tccgatgtct ttgatgtcat tggcgtttac aagcaacgcg
    25981 ttgacgagaa tcaggaaaag atcaatcgga aggccgccaa aagaatcgcc gacatggcca
    26041 gcaaactagg caactatctc ggatattcgc ccaagttatg caaggagatt ttcgattttt
    26101 ccactatcca tatcgacaag ataagttatc tgtcggtgag cgattacgac gacaaggaag
    26161 ttttggacga gaacgactac cggaagatca gggaaaaaac gatcatcgga tccgtgatca
    26221 tcaaacggct gcagttagag aagaagggcg aagacattgt ccgggctcat tttgagaaaa
    26281 cggccgacaa ggattttatc agagacatga attcgattca ggacaaccgg gaatcccaag
    26341 tcatcctgtt atgcgaaata tacgatatat tgcgccagcc gcgcaattac aaacgggaaa
    26401 tgaaacattc ccgggccatc gatttgatgc aactggaatt ctacccgtac ttcgatcctc
    26461 agatcgtcga tcgttttgtg aaatacggag atgagttcga atcgctttac tacaagcttt
    26521 gccaagaata ggagataatg cccatgtatc gtttttttac ttccgaatcg gtaaccgaag
    26581 gacatccgga taaaatatgt gatcaaatca gcgacgccat tctagacgcc attttggccg
    26641 atgatcccaa cggtcgcgtc gccgtcgaga ccttggtaac aaccggtctc gtgctggtgg
    26701 ccggggaaat cacgacttca acttatgtcg atatccaaaa aatagtgcgc gagactgtcg
    26761 ccggtatcgg atacgatcgc ggcaaatacg gatttgacgc cgaaaatctg gctgtgttgg
    26821 tcgctatcaa cagtcagtcg ccagacatcg cgataggtgt caacgaaagc gtcagccacg
    26881 agatgggagc cggagaccag ggaatgatgt tcggctatgc tattgacggt aacgacgaat
    26941 acatgccgat aacccatgtg tatgcgaacc gcttgtctag aagactcgct gaagtcagaa
    27001 agacaggcat cttgcattat ctgcgcccgg acggcaaaac ccaggtgact ggcgaattcg
    27061 acagcgaagg caacttagta agattcgaca agattctggt ttccactcag cacgctccgg
    27121 aaatcactca ggatacgatc agaaaagaca tgataaaata tgtcatcagt gaagtcatac
    27181 ccgaaaatct catcgataag gataccgaga tactcgtgaa tccgaccgga cgtttcgtca
    27241 tcggcggacc gtcgggcgac tccggactta ccggaaggaa gatcatcgtc gacacttacg
    27301 ggggcgccgt gcatcacggc ggtggggctt tctcgggaaa agatccgagc aaggtcgaca
    27361 gaagcgccgc ttatgcctgc cgttatgtcg ccaaaaacgt ggtggcggcc ggactggcaa
    27421 aggagattga aatccaggtt tcctatgcaa tcggagttgc caaaccgaca tctatcggtg
    27481 tgaacacttt caataccggc aaactgccgg atgaaaaatt gatcgaaatc atcaaaaagt
    27541 gttttgattt tcgtccaagg gcgatcatcg actatttcca gctttgccga ccgatctaca
    27601 aacaaatttc cgcctacggc catttcggac gtacggatct ggatttgcct tgggaaaaac
    27661 ttgatctcat accgacactc aagaagcttg aaaaagaagg ttgatcatga tgtttctcgc
    27721 cacaatcaaa caaacaatcg aaataattat cgtcattacc gtaccgttgg ctatccttgt
    27781 cactggaaat gttttgatga taagatctct taaaaccggg tacaaggatg tctataagtt
    27841 ccagtctaaa tttgatatcg aactgcgaaa gactatcaat ctattttcca aaatcgaccc
    27901 agagggtatt ttgaaagact atcgcgaaac agtcattaaa aacatctccc acgaggaaaa
    27961 gatcgaactg ttggatctgg tcgacgagga atacaaactg atagacaaag aatccggaaa
    28021 caacgaatat gcgatggaga catatgaaaa tctacaggag atgcggcgga ttcatgacac
    28081 caaggctctg attttcaacc acaagatctt gatgtttcct ttcaatgtct acgccagatt
    28141 gtttaaaatc aagaagtggg atttgtatct gcatgaaaaa aaggaagagt cgtgagactt
    28201 gcttcaagat aaaaagaaaa gccgcaatcg gcttttttta ttccgctttg ttgatgacaa
    28261 tgatatatgg ggcgagttcc ttgtttaaaa cggaatgttt cgatacatca tatgtctgtc
    28321 ccggcagtct ttcgacgaaa cgcaacaata actggctttc gttgccttcg tgtttctgat
    28381 agacgacaat gacgacgatt ccgcccggcg ataacatctt tagtaacttt tcaagcgatg
    28441 taagtgtcga ttccgccgta gtacggattt tcttctttcc tcccggcaaa tatccgagat
    28501 tatagatcgc tccttgtatt gggactttga tgtattcgtc gattttcgca tggtcgtcat
    28561 tgatcagttt cacccagtcg acatggtttt tccttaacaa ttcgcttgtg gttgcaatgg
    28621 cttcttcctg gatgtcgaaa gcatagacat catggctgag cgaagccaaa aaaagagtat
    28681 cctgcccttt tcccgccgtc atgtccacaa gagtcttgcc ggatagttta tcctttagaa
    28741 gccattcatg gctccatttc gccaccgtaa tcatccttcg tatctcgctc cctgatggct
    28801 gtgtcttttt cttaacagtt tgtcgatgtc gtttgtcacc gtgaattttt tcaggctcca
    28861 aaggggagcc accaattctt ttctcgggct gtctcccgtg agtctgtata tgatgatgtc
    28921 cggtcgcaac aattcgatct gatcggct
//
LOCUS       DAOPJL010000015        28563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1754_length_28563_cov_8.228146, whole genome shotgun sequence.
ACCESSION   DAOPJL010000015 DAOPJL010000000
VERSION     DAOPJL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 28563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28563
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_28563_cov_8.228146"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1993
                     /locus_tag="PLP02_03150"
     CDS             <1..1993
                     /locus_tag="PLP02_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42482.1"
                     /translation="MKRKLIAIALLAFVSFAFVACNNGTELTTGSTTTTTTNGLTTTT
                     TTTTTTTTTTAIPFDRTLLVDYVTTKMTVIIGEITYTPTSAEVEAEIDYYQGMFGDVD
                     EETVYGLLIGIDTLESTFEDSTDTLDDLQNWYQSAIDYGINKTFVANTLVNALTDYIQ
                     QQIDAFDLSELLEAISAKEDEIEAYQLVLIGIDSAAAQYIDGLDPEYQEIAQQYYDGF
                     TDLTSDLELYDNLYDYYVSDLYDEDWDFIAYYAMQNNLYNYYYTLYILKNEVDANDYL
                     TDYNNQYDLLSVAEKEMYGPVLEAYRQYLIDVYAYYNGALNSLDGVLDGNSQPITTYI
                     YNNFLDSYSLNYDAMQSCLSALEDMNDELTQGNHLIDFVSDFKAYLLTTEGKANAIAL
                     IASFYDILDYAVTNFDSDTYDLIMEIANEDISSFPFLSEGEFGLEFDPDLLTPAHIAS
                     YSVMISGIFGNLFDTIDESDMDNFEVVVIDILEMYFENFEYSTEDTAIYMNLVEVILQ
                     NYIPALVGDFADFGTVLGTVDNTVANDIYDAFILIYNEDKETPDNETVIAAAIVIDDL
                     LGGADPAFDLADALCDISGLYYTVINTLDPEEHVNIIEIYSEIDNLVSDILTYSASVA
                     GYDAGTITSIQQDEIDYLVSCIEDLSALLGEGIGHYFYR"
     gene            complement(2051..2902)
                     /gene="speE"
                     /locus_tag="PLP02_03155"
     CDS             complement(2051..2902)
                     /gene="speE"
                     /locus_tag="PLP02_03155"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPF42483.1"
                     /translation="MKLTDFTEHWEKDVDFSIRNVKHLYSEKTPFQQIDFYESDTFGR
                     FFTLDGYLMLTEKDEFHYHEMITSVPMAVNPDIKNVLVVGGGDGGTARELSRYKSIER
                     IDVVEIDERVVRLCEIYIPQTSSVFSEDSRVHLFFCDGVRYLKNLPEKSYDLIIIDST
                     DPIGPGEGLFTKDFYLDCRKALSEDGILINQHESPYFDKDRIQMQKAHKKLTECFPVA
                     KVYMFHQAVYASGLWLFGFASKKYDPIKDHKPETWTRFGIKTRYYNNALHKACFVLPT
                     YVEKLLE"
     gene            complement(2910..3710)
                     /gene="speD"
                     /locus_tag="PLP02_03160"
     CDS             complement(2910..3710)
                     /gene="speD"
                     /locus_tag="PLP02_03160"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPF42484.1"
                     /translation="MEKLALYGFNNLTKTLSFNIYEVCITETENERRQYIEYIDERYN
                     SHRLTEILIQLSANIGANILNISKQDYDPQGASVTMLISEEPVSLGSIDKSCNLGLLP
                     LTNETVLAHLDKSHITVHSYPESHPGRGISTFRVDIDVSTCGKISPLKSLDYLIGSFD
                     SDIMTIDYRVRGFTRLDSGKKVFIDEKVDSIQDYIRKKTLDRYDSIDVNIYQENIFHT
                     KMMVKTLDLDNYLFERKASDLPKEKQMKLRKTLKREMKEIFYGRNLYE"
     gene            4069..4308
                     /locus_tag="PLP02_03165"
                     /pseudo
     CDS             4069..4308
                     /locus_tag="PLP02_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            4492..5049
                     /locus_tag="PLP02_03170"
     CDS             4492..5049
                     /locus_tag="PLP02_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPF42485.1"
                     /translation="MRTQLMMKKLCEHLSQKAKYRDIMTEGYIMELTTTTPLHDIGKV
                     GVPDSILLKPGKLTPEEYEEMKKHVGYGVTALKSNLDPTAETPSFINTALEIINYHHE
                     KYDGSGYPNKTKGKEIPLPGRLMAIIDVYDALISKRVYKEAFSFEEANAIIRKDTGSH
                     FDPDIAEAFLEITSEIEKIVLPYMN"
     gene            5075..7951
                     /locus_tag="PLP02_03175"
     CDS             5075..7951
                     /locus_tag="PLP02_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPF42486.1"
                     /translation="MRKILLVFMLLLSSSLTFINLSAATTENEPIVWTTEEAAFLADH
                     PEISVGIDTESAPFEFIENDQYKGLTADYLEIIAEKTGIEFQIVYNDTWEETYNQAVA
                     GDIDMLSAVSKSHEREEIFLFSEMYYELRRVIVVMESNTDIHGIEDLYGKTVAVHAGS
                     SHYDFLLDYPEITISSYSTVLAAITDVYQGYAVAYIGNLATSDYLIKSHGISGLRYVN
                     LEEENPAGLYFAFHQDWTVFAGIIDKVLLSISAEEKIQINSRWMTIDYTTDYGPLLKA
                     IGIASAILLLGGLLSFFWNIKLKKEIDIRKRTQVELERSKQEAIEANNIKSSFLARMS
                     HEIRTPLNAISGMAYLLKETDVSTTQMMYAERITQASDTMLGIVNDILDYSKIEAGKA
                     SIDLISFNLDHIIQNIISIISVKITEKKLRFRFHKDPGIPVWLEGDCKHFQQILINIL
                     TNAVKFTETGEVSLTITEKARNDSIITLRFSVKDTGIGMSKATLENLFTPFTQADSSI
                     TRRFGGTGLGLSIARNLAEAMDGKIEVESLESAGTLFTVTLPFTIDIAKNKETKTEKS
                     EQYLKKLNVLMLLAAKSDMDIMESYLTSFGIEATSTISPEEAMHLVVNEPGKYDLLII
                     DYEAPSGHGFSYLKQLRETVKISEFPKVIMLLPMSRSDLYNHLEVNFVNLGIGKPVIP
                     SVLYNGIVDIFINSQIKEKGKQFKNDLKLESTGYAKPLVLVVEDNQTNQMIARLLLEK
                     SGYLVVSAENGREGVAAYAANRDLIRLVLMDLHMPVMDGFSAAKLIKQEDPDIPIVAM
                     TAEVIPGVREKCEANGIDHYISKPFDPEKFIETVNNILGISNFSKAKKALHINKEKGL
                     KLTGNNENLYQLILKTYREENIDTGQRLREVIAKQDYSAARQILHKIKGSTGSIGADK
                     LFQIITDLQKAIIDNDEVMITDLSTLFADAIQVVLAEIAENTSK"
     gene            8007..10874
                     /locus_tag="PLP02_03180"
     CDS             8007..10874
                     /locus_tag="PLP02_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPF42487.1"
                     /translation="MQRKILSLFLLGFLFVMPWRVEASDSTIEWTQEETEFIASHPVV
                     RIGVDPEFVPFEFIDNGEYKGIASDYVSIIEEKTGIDFQVTPGLSWPKAYDEAVEGNI
                     DVLPAISKTPAREEIFLFSEAYYEVRRVIVTRNDNTSIKDLDDMYGKIVAVQINSSHH
                     TFLLQYPEISLSLYNTVNDAIAAVSAGTEEVFVGNLATSVYIAESFGISNLKFFVLSS
                     EEPVELHFAIRSDWPVLVDIIDKALESITIAEKQEISSEWITIDITPDYGPFIKSVAI
                     GAAILLTGLLISVFWTVKLKKEVAIREKTQKELETAKRDAENANNVKSGFLARMSHEI
                     RTPLNAISGMSYILEKDCQTPTQGMYAKRISQASEAMLGIINDILDYSKIEAGKTSIE
                     VISFNLDQVIQNTISIVSVRISEKKLGFRFTKGNGVPVWLFGDPKRLEQILINILNNA
                     VKFTDKGEVRLNIDVKSNDNGIITICFTIADTGIGMSKETLDNLFVPFVQADSSITRR
                     FGGTGLGLSIAKNLAELMGGIILTESVEGLGTTFKISLPFEVDVMKEENRCKEEINQE
                     IKNLNILVLDDNPYDLDIIASYLSAFCLRAQTAGSVYEAKQILEDGAKKGGNQIDILI
                     VDYNLKLEDGFAFVRSLNNETMKDKMPKIIMMFPFDRPELFDLMGKSGISEGIDKPII
                     PSVLLNKIMQLASLGKSSADNEKHDETFAIPKEKALILMADDNETNRMITKILLEQDG
                     YRVIAVENGKLATEAFAEEGKNISLVLMDLNMPVMDGCEASDLIIQMSPFIPIVAITA
                     EATPGVREKCREHGVFHYISKPFDPKTFPSFVDKIIDNNITRSMEPQIDKESGLARNK
                     GDMEAYLDSLKRFANENVNAFEDLWHAIEKSDCQKAESAVHRVSEGATLIGAEKVGPT
                     CKEILNEISVRNRSEVIRLAEKFEWQLEMIMREIISELRKK"
     gene            complement(10913..11731)
                     /locus_tag="PLP02_03185"
     CDS             complement(10913..11731)
                     /locus_tag="PLP02_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPF42488.1"
                     /translation="MGKLGLCLSGGGAKGSYQIGAAMVLKEQGIFDRIDAFSGTSIGA
                     ANVCLLASTSVEAARDLWLSMPDNALERNRTRGHASSLKEKFNMIDHGLFKIDPLEDL
                     LLASVDQKRLNEREVYVTVSELESEDSGFAAFLRMGFRRYLHDDSKAFYLPLREMSDN
                     ERLKAVVASCSIPVIFPAVIAADDKKYYDGGVYDGVPIKPLAEAGCDTIIIIRLNPRA
                     PYHPELYPGIRFYDIIHKTSIGSPLDFTVDHVKELYQLGYNDMSDYLKTLYVQP"
     gene            11893..14025
                     /locus_tag="PLP02_03190"
     CDS             11893..14025
                     /locus_tag="PLP02_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPF42489.1"
                     /translation="MGLTVFLFILAYLLLNLWNAVVLTHQKLNRYIVPFRHTLLGELN
                     AILANTAVLGLFLMIVLVFTKNAYALCLVLVILTGVFNTYFLGLNIFNLYYGTSFSRE
                     DLEIFRNPGEGIAKGMGGELIRELFVYYRILLFLPFAFLLVFFLKTDPILLSAYRVPI
                     SNGAVFTMIAIFILVLFLTGTLFEKMYAKQLMIQVLKSTYAIQNFGVYPYYLCNFTGI
                     TKLIDAKIEKRSGPPSDLLPTFQKYNKNKKKYVNLLDGKTYSNSMSTDDLSENIFVDP
                     SLIKEDKSLDGILKGRNLVLVQMESMNRYLLDIGLTKDEFSFLKALLPEALDLREFYT
                     SVGIGVSSDAEVTTLTGLWPTGRSNLYWADYDRKTQKYLVKRDLSTIVKYFKQDNYST
                     KALHGDYKRFYNREHSYGEIIGFDEFQAMEDFTEIKECEKTGILKLYSYEYADGKEHV
                     SPWVSDYLLAEKVRENIHEKQGPMLLYPITMMPHIPFEFYPGEMNKALKDSQLKEQTK
                     KYLRFADYYDDFIRRFFFTRDGQIDTDPNTVYIFYGDHGSGIKNGDIEKLVDRKLTKM
                     EERKILIQIAGFIYVPGSDFVERNGIRIRKGLLKGHQDLVRGQMDLNRTIIDLFGLNG
                     EKDAYFGVSLLSKEPTFILDNKLQDVVTDKVMFSMRNTENTFPSGVEIGKGMFDIVKE
                     FKLMNERLLQKPECEKKLNERMHASGSE"
     gene            14152..15282
                     /locus_tag="PLP02_03195"
     CDS             14152..15282
                     /locus_tag="PLP02_03195"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HPF42490.1"
                     /translation="MTFKNISRRVLIVILSTFYVLITLFNVIVLFFCSLRFTDNRQQI
                     NHLDDLQEYYETTPSSEIAAEQSFVSFDIDFNDLRLNEIKMVASHNSYKKQGTALGRA
                     MIALGSSKEDARLMNYGNVWISEQLDAGIRSIEIDLRYKNGSFVTTHVPLVDNRSVCP
                     DFSLLLEELKLWSDNHPNHIPIIIILELKSDWMVLDPTLDDIGQSELERLNDMLLFNF
                     DSAIYTPEMLKGSYDSLQERIATESWPYVSDLLGKFIFVLHPGEFTDTYVSLFPDYEG
                     MSLFPAVSNLDTDHGYAAFVVHNDPNIEEINALVAQNYIVRTRMDEDLVNDPDRMADA
                     IASGAQILTTDLHPATQIQNATPIYLDGNYTVIVNDITHEDE"
     gene            15314..15859
                     /locus_tag="PLP02_03200"
     CDS             15314..15859
                     /locus_tag="PLP02_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42491.1"
                     /translation="MYSETSAQRIKEDIFLIDNSLKERVPSQARSLLSILMRTYDGIL
                     MSLNTAMLPTESPIYGHNFDELKNYDENVFSNLTVIRNKLQLFDLQFQNVLEKNLEAG
                     NTGYLSQNEIVNAIKVCKNVDAKEKEDALKMAEILKDIAEGDNLGFQAKWERIKPVFA
                     YLADANPNLGSLVMELLKRMA"
     gene            15888..16601
                     /locus_tag="PLP02_03205"
     CDS             15888..16601
                     /locus_tag="PLP02_03205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPF42492.1"
                     /translation="MADFTSRLKKYIQLCPMTSGLLTVNTAFLIITIITGGSEAFGSD
                     NLVRLGGIVPSYVASGEYYRIIFAMFLHGGYLHFFSNMLALYFLGTPMERIMGGWRYL
                     ALYMLSGIGAGIMIVLFGYSNITFLNTYGEVSIGASTAIYGIMAGLVFITIKRPGWFP
                     RQTVKSIWMIAVLNFIITFIFPYISVVGHLSGFVFGFVLSLVLMPKIPEYQRKAVIAK
                     YFQKKATSENDNDDDDYHY"
     gene            16680..17465
                     /locus_tag="PLP02_03210"
     CDS             16680..17465
                     /locus_tag="PLP02_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42493.1"
                     /translation="MTKKHYTLLNLAIFIPMGLALLSFGFAIYFQSNGYAKEIWVPLA
                     LSVFCFLAVSLIFLFVNYPALIEYRTKKKYEELKKRPLCFAYQSETIESLTRRLGKLG
                     FYPDKNGMLYNKIPYLLTGSIRFYIAIVDADENPAYFENIIAKYHSDPIYQSEKGYRC
                     LTVIGMKKKLSRTDAAKIKKYTDEHLTLDSALFGSSAEASFWMLYESGKGRFLFKNVG
                     NGSKIKAAFIGGWMLKKLLLLNNTEIVCETDDPFDSTLLSPEK"
     gene            complement(17514..17864)
                     /locus_tag="PLP02_03215"
     CDS             complement(17514..17864)
                     /locus_tag="PLP02_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42494.1"
                     /translation="MKKHDWLFWLPRLFTVIAIGLVFATSFDSFGTGNGFWNDMQGFL
                     IHNIPTAVLILIFILTWKWEIVGAILFPLAGIAWITFLSIRNIEMLISGIIVAAPFIV
                     VGILFYFDWKRQTV"
     gene            18020..20512
                     /locus_tag="PLP02_03220"
     CDS             18020..20512
                     /locus_tag="PLP02_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPF42495.1"
                     /translation="MDKSAGIKQPKNLTKRIEWLRNYYFEGVKRDWNNEYHCYTTGKP
                     WDFLFDEITYYIVPETIPFYQTFASSSLQSAKTIALPDDFWKLSLKERRAIFMKEAVV
                     NNMPADLLKGDLICGACFNLLTSHCLTKKEAKDRNHLVYGKNGSRNTVLDYYYYGFGN
                     TGSTCGHLIPDYAKIVKKGFRAVKEEIENEYDSLSEKKKKSSEGEQLRAMMIACEMPR
                     ELAAKYREKCLNEAKTEKDESRKKELELMAENLLVVPWEGAKDFYQAIQSLWITHMLV
                     MSDENYPGPGVSFGRLDQYLYPYYEKSRKNGMSDDFMKEILECFWFHCNTAYDAQIRV
                     GNNGITAGFGQLFNISGCDKDGNDLSNDLTYLLLDVIDDMSPILEPKPNVRLHRNSPE
                     KLLDRVVDMVVSSQGAPFLINFDERSMAGMMREGRICHLDDYINLNNVSDYASVGCLE
                     NTMQGNDRSGTVDNNLYFYKAIELALNAGKDLTPKHNDTDGRTLPIRQNGVYTDKVED
                     LGSFADFYEAVKRQTAHIIRKSTEVYEKSEKIRCKYGNTPYLSTLVDGTIQSHKDVTE
                     GGAELRFVTIEGVTFATTIDSILAVKYLVYDKKECDLKTLNEALKNNWEGYEILRQKA
                     INKAPKYGRNDEEADEIAKDYMNFFAEETWKHKTKSTNRQFRPGMLSWNYWASAGFIL
                     PASPDGRKQGEFFSNAICPVNGRDIFGPTANSNSVGIALGGKAEAGDFKDYVNYLPNG
                     ASHTITFNSSMLANPDHKEKFKSFLRGYIENGGTALQINILDPEMLKDAQKHPENYKH
                     LLVRVTGYNAYFVTVGRELQNEIIARESHKEY"
     gene            20515..21441
                     /locus_tag="PLP02_03225"
     CDS             20515..21441
                     /locus_tag="PLP02_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPF42496.1"
                     /translation="MRIINIQRMSTEDGPGLRTTVFFKGCPLSCKWCHNPESLSYKIG
                     KEWIAQYCMFCGTCVAKCPENALTITNNEVRIDSEKCKMCLECVENCPTGAMKSLGKD
                     IGIEELFRELIKDKAYFRATGGVTFSGGECLLQTDELVELAKMLKKENISIAIDTSGA
                     INYHLIEKILPYTDIFLYDIKLIDKAKHKELCGKDNQEILSNLIALSKAKADIWLRTP
                     IIPRATATNDNIRAIAGFLRNNHIGFSRWELCSFNNLCKSKYERMSIDWEYKDDRLMT
                     NEEMEELLASARQEYGNPEATILATGSRKTEE"
     gene            21444..>22143
                     /locus_tag="PLP02_03230"
     CDS             21444..>22143
                     /locus_tag="PLP02_03230"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF42497.1"
                     /translation="MNDKLTPEMIEEFAKSVKVGLVASIDEKNSPHITVLSTLMGKGE
                     NTMVFGKFIEGLSKEFIQERPRAGFLIMNPEKEFWYGKMIYESEKKEGEEYVFYNNQP
                     LYRYNTYFGINTVYYSKLVCISDMDTLPLGKIIASSLSVMLNKTRYKGSAKTEVMKPW
                     AKKFTAKLDTLMFLSYMDIEGYPVIVPIIQAQSASTDRIVWKNKPFGERQLSLKPGQK
                     IAILGFSMSMETVLL"
     assembly_gap    22144..22243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22408..23115
                     /locus_tag="PLP02_03235"
     CDS             22408..23115
                     /locus_tag="PLP02_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPF42498.1"
                     /translation="MLKNASKTEKVVESIRNRIISGELIPGDKLPPLRELVSIYNASR
                     SVINQAIGTLSAKGYLEVKPRQYIMVNDIWASGNLEIVNDVYFAPKSRYKSRAIREML
                     KARKMAEMASLGEITPDNIPNLSQLKIIYAEEKKWLGQKSADIGIIADLDSKFHEEVV
                     SLSNNFVLSLLYLAFKNIGDDLIRKFYFCVNETDCPINWHGQFIEALENKDIGNAKII
                     WLKLLSKGEEVILQNID"
     gene            23215..23439
                     /locus_tag="PLP02_03240"
     CDS             23215..23439
                     /locus_tag="PLP02_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42499.1"
                     /translation="MILGNNLRRLRFENGEMSQQDLADLVGVSRMTIYSIEKLKYTPS
                     ASLALKIANVFKCPFDKVFFFKTKTIGEDE"
     gene            23432..24337
                     /locus_tag="PLP02_03245"
     CDS             23432..24337
                     /locus_tag="PLP02_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42500.1"
                     /translation="MNEMTSRQIKLVIFGGVTLLCFVYVAVAFAIIKNQLVPSLAEPT
                     LLINYLSYGIMIAFVVFGVYCLLMLRDALIQIVQIRSGIIYPSLLVVLTVLGGVAILA
                     DITLLSDIGKEYLQFDVAGEWKMLFGIWAFQTVTIIFQIVMSCCHDVAGNKLTEKLQH
                     MSDSLYVSTFQVMLISSILGLISIFTTFFWANRLPELSKYATGLMIVLCILSVLPAAV
                     IICHLIIRNRRRGFNDWFDERQWDNLSKGGMVTVITGVCFLILSIIVSQLASGIDPGY
                     LLLTVLFAEIGVFSAFVLSRNRIDD"
     gene            24441..25565
                     /locus_tag="PLP02_03250"
     CDS             24441..25565
                     /locus_tag="PLP02_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPF42501.1"
                     /translation="MKKYFMYFWKDKVFVITFVFTAISCFFITPSMEYLDYVDWKVPF
                     IMFSLMVAVAGMYEADLFGYMSIKMVLRFHTLRWIALSIIMASFFLGMLVTNDAVLLT
                     FVPFTILVTKQTGQEKYAITIIALQTIAANLGSALTPMGDPQNIYLYAYYGIRFGDFL
                     RVMAPVAIGGFFILITMTGAFVPNIFSEPVMIAPKINRKKTIVALMIFLNALLCILRV
                     YSEWIAIIVTVVMVLIFDRKLLKKPDYTLILTFIAFFIFTGNLGKIPGIVSFVSGRLE
                     NGTSVYLSGFFLSQFISNVPSSVLLATFTKQEYWPYLLQGVNVGAMGTLIASLASLIA
                     LKFVLKEFPRQGKEYILTYSFFCFIAILVITMAVFIFDGI"
     gene            25674..25994
                     /locus_tag="PLP02_03255"
     CDS             25674..25994
                     /locus_tag="PLP02_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF42502.1"
                     /translation="MMNSQFKKGIIEMCVLELISHRDMYGFEVIERLSEDIDVNDNTI
                     YPILRRLTEQGYFDNYVESTSMGAPRKYFRITEKGSGKLTEYKTEWDKFLKGVSRIME
                     EKAK"
     gene            25991..27088
                     /locus_tag="PLP02_03260"
     CDS             25991..27088
                     /locus_tag="PLP02_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HPF42503.1"
                     /translation="MKKEYLAKLKQKLEEKDFDKSEINDIIDDYDRMYDDALLKGMED
                     ADIMNFLGDPDRIAFDLCDGKIRKQNRKNNGKIVAIMPFVSLILFFIMGYAFHLWHPG
                     WLVFLLIPVTAIIAGARKDKNGTLIALMPFIATAIYLGFGFGMNLWHPTWLIFFLIPV
                     TAICSGQTRLLGKLTALTPFIAVTAFFILGEYGYYHPGWLVFFLIPMVGVLNEKHVWK
                     AVIYEASFVIAIALYLAIGYLNPIWAQAWLVFLLPVIVSLLFGEYNFQIDGMSVGIRI
                     LTIVLIVIYVLFGFLFQTWSYLWLIFLLIPVAAIIGNGKGMQKKLVALSPFVATVAFF
                     LLGYLGGYWQYSWMAFLLIPMTAILTKGKEE"
     gene            complement(27128..28114)
                     /locus_tag="PLP02_03265"
     CDS             complement(27128..28114)
                     /locus_tag="PLP02_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42504.1"
                     /translation="MKGSAFMKSHKWLRLSSALLVTIAFLGFVYLRFVFTNSVGPYFL
                     KFLYYVIGIGIPIIILSWMKKRPLVPVLALGIAYFGYFIYILFLIIRPDLTIHTQYEV
                     AFLFFYIGFEQADLSRINTLASGNVFFYIGYILATGTILTSLIYMPFSIKPMNKPRQS
                     VTLSLILGFLLVPILACLSILYSFYNSNPGYISSERNLLVNRLNIVVFVGALLFVILF
                     WIFRSKALPDDSGHSLRVRMEIVFPLGFYASLLVPAYVLFYGINTFYWFGVIFLFFVL
                     LLTAGFGWFIYKKSSKWKKETIMAFCVNYLVLMIPLCYAVLLLSLLSGGSSV"
     gene            28225..28298
                     /locus_tag="PLP02_03270"
     tRNA            28225..28298
                     /locus_tag="PLP02_03270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28257..28259,aa:Gly,seq:gcc)
     gene            complement(28336..>28563)
                     /locus_tag="PLP02_03275"
     CDS             complement(28336..>28563)
                     /locus_tag="PLP02_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPF42505.1"
                     /translation="QYFSKQTGMTLQKCILKMKIHESENMLRYTHQSVGEISTSLAFC
                     SQSYFTEVFKKINGMTPADFRKRYKQSIKSE"
BASE COUNT         8425 a         6067 c         6410 g         7561 t
ORIGIN      
        1 aatgaaaagg aagctcatcg caattgcttt gttggcattt gtcagtttcg cctttgtcgc
       61 ctgcaataac ggcacggaat tgactactgg gtcaacgacg accaccacga cgaatggtct
      121 gacgaccaca acaaccacta caacaaccac taccaccacg accgctattc cattcgaccg
      181 aacgcttctg gtcgactatg tgaccacaaa gatgactgtc attattggcg aaatcacata
      241 cacgccaacc agtgcggaag tcgaagcgga aatcgattat tatcaaggta tgttcggcga
      301 tgtcgatgaa gaaaccgtat atggtttatt gattggtatc gacactttgg aatccacttt
      361 cgaagattcg acggatacgc ttgacgatct ccagaactgg tatcagagcg ctattgatta
      421 cggcatcaac aagacttttg tcgctaacac tctcgtcaac gccctgacag actacatcca
      481 gcaacagatc gacgccttcg atctttccga attgctggaa gcgattagtg caaaagagga
      541 tgaaatcgaa gcttatcaac tggtgctcat agggattgat tcggctgctg ctcagtatat
      601 cgacgggctt gatccggagt atcaggaaat cgcccagcaa tattatgacg gattcacgga
      661 tctgacctct gatttagaat tatatgacaa tttatacgat tactatgttt ctgatttgta
      721 tgacgaagac tgggatttca ttgcgtatta tgccatgcag aacaatttgt acaattatta
      781 ctatactctg tatattttaa agaatgaagt cgacgccaat gattacctga cggattacaa
      841 caaccagtat gatttgcttt cggttgccga aaaagaaatg tacggtccgg ttttggaagc
      901 atacagacaa tatctgattg atgtttatgc ctattacaac ggagcgttga attccctgga
      961 tggagttctt gatggcaatt cgcagccgat aaccacttat atctataata attttctcga
     1021 ctcgtattcg ctgaattatg atgccatgca aagctgcttg agcgctttgg aagacatgaa
     1081 cgacgaattg actcagggaa accatctaat tgattttgtc agtgatttca aagcatacct
     1141 tctgactacg gaaggaaagg ccaatgccat tgccctgatc gcttctttct atgacattct
     1201 cgattacgcc gtaacgaatt tcgattccga cacttatgac ctgattatgg aaatcgccaa
     1261 cgaagacatt tcgagttttc cgttcctttc ggaaggtgag ttcggcctcg aattcgatcc
     1321 ggatttgctt acaccggccc atatcgcctc ctattccgtg atgatatccg gaatcttcgg
     1381 caatcttttc gacacgatcg acgaaagcga catggacaat ttcgaagttg ttgtcatcga
     1441 tatcctggaa atgtattttg aaaacttcga atacagtacc gaagacacgg caatctatat
     1501 gaatctggtt gaagtcatac tgcagaatta tattcctgct ttggtgggcg attttgccga
     1561 ttttggaacc gttctcggca cagtcgataa cacagtggca aacgacattt atgatgcatt
     1621 catactgata tataacgaag acaaggaaac accggacaac gaaaccgtaa tcgcagccgc
     1681 cattgtcatc gacgatctgt tgggaggggc tgatccggcg tttgatttgg cagacgccct
     1741 ttgcgatatt tccggtcttt attacacggt gatcaatact ttggatccgg aagaacacgt
     1801 gaatatcatc gagatctaca gcgaaataga taacttggtt tctgatattc tgacttattc
     1861 ggcatccgtg gccggatatg acgccggaac catcacatcc atacaacagg acgagataga
     1921 ttatcttgtc agttgcatcg aggatctttc cgctttgtta ggcgaaggaa tcggccacta
     1981 tttttacagg taacataaca atcaaacaaa aagccactcg cgagtggctt tttttatctt
     2041 tatttgattg ctattccaac aatttttcca cgtaggtagg caatacgaaa catgctttgt
     2101 ggagggcatt gttgtagtat ctggttttga tgccaaatcg agtccatgtc tccggtttgt
     2161 gatctttgat cggatcgtat tttttcgatg cgaatccgaa cagccagagg ccggacgcgt
     2221 atacggcctg atgaaacatg tatactttgg caaccgggaa acattcggtc agttttttat
     2281 gggctttctg catctggatg cgatccttgt cgaaataagg cgattcgtgc tggttgatga
     2341 gaatgccgtc ttcggaaagc gctttgcggc aatcaagata aaaatccttc gtgaaaagac
     2401 cttctcccgg tccgatcgga tcggttgagt cgatgatgat gagatcatat gatttttcag
     2461 gaagattctt cagatacctg acaccgtcgc aaaaaaacag atggacgcga gaatcttcag
     2521 aaaacacgga tgatgtttgc ggaatgtata tttcgcataa tctgacgacc cgttcgtcga
     2581 tttcgacgac atcgattctt tcgattgatt tataacggct taactctctg gctgttccgc
     2641 catcgccgcc gcctactacc aaaacatttt tgatgtccgg gttcactgcc atcggaacgg
     2701 aagtgatcat ttcgtggtaa tgaaactcgt ctttttccgt cagcatcaga tatccgtcca
     2761 aagtgaagaa acgcccgaaa gtgtccgatt cgtagaaatc gatttgttga aagggtgttt
     2821 tttctgaata cagatgtttg acgttgcgaa tcgaaaaatc cacgtctttt tcccaatgtt
     2881 ccgtaaaatc agtcaatttc atgtctatct cactcataaa gattccgtcc gtagaagatc
     2941 tccttcattt cccgcttgag ggttttcctt agtttcatct gtttctcctt gggcaaatcg
     3001 gaagctttcc tttcgaaaag ataattatcc agatcgagtg tcttaaccat cattttggtg
     3061 tgaaaaatat tttcctggta aatatttaca tcgatgctgt cgtaacgatc gagagttttt
     3121 ttccggatgt aatcttggat cgaatcgact ttttcgtcga taaacacctt tttgccactg
     3181 tcgagtcttg taaaaccccg gacacggtag tcgattgtca tgatgtcgga atcgaaactt
     3241 ccgatcaaat aatcaagact tttcaaagga gagatcttac cgcaagtcga aacatcgata
     3301 tcgacccgga aagtcgagat tcccctgccg ggatggcttt ccggatagga atgaaccgtg
     3361 atgtgggatt tgtcaagatg agcgagcacc gtttcgttcg ttaatggcaa caggccgaga
     3421 ttgcatgatt tgtcgatgga accgagagat acgggttcct cgctgatgag catcgtgacg
     3481 ctagctccct ggggatcgta atcctgtttg gaaatgttca ggatgttggc gccgatgttt
     3541 gcggacaact ggatcagtat ctctgtaagc ctgtgggaat tatatctttc gtcgatatac
     3601 tcgatatatt gccgtctttc gttttccgtc tcggtgatgc agacttcata aatattgaaa
     3661 gacaatgttt ttgtcaggtt gttgaaaccg taaagagcga gtttttccaa tgtgcctata
     3721 ctccgttttt cattttatca ttaataattt atcattattc ggaaacaatt tcaatctatt
     3781 ttatccgcca tcggccaata tgggaatatt cttgttttat tttggtgctg atgtgttgtt
     3841 tttatataaa tagtaatata taatgaaatt ataatacggt caacattttt ttatcacgaa
     3901 aaatttgaat aaaaccttcc ccatcacgag tatgcaaaat gatggggttt tatgtttttt
     3961 ctgggagggg aagacatgcg tacgaaaata ttgatagtgg acgattcatt gacagatctt
     4021 gccatcatcg aaagcatgtt gcggtaattc gatacaagca cggcgaaaaa cggtaaggaa
     4081 gccatggagt ttgttgaaaa aaatcttgat ctggatttga taatccttga tctaaacatg
     4141 ccggtgatga acggtttcga agttttgcaa aagctgaaat cggatgaaaa atatgccgga
     4201 atccgggtta ttattctaac caattatgat gaaatcgaca acgaaatcag aggattggaa
     4261 ctgggagcgg tggattatat ccgcaagccg gtcaacattc gttccttaag aatccggatc
     4321 gacatccatc taaaactgaa acaaatgcaa agacaggtgg aagaagacaa tgttgttttg
     4381 gatcgaaagg tcgctattgc gaccaaggaa cttgtaacgg cgcgcgatat caccattcag
     4441 gctttggtcg gattgcttga ggcaagaaac ctggaatctt acaatcacac gatgcgaacc
     4501 cagctgatga tgaaaaagct ttgcgagcat ctgagtcaaa aagctaaata tcgcgatatc
     4561 atgaccgaag gatacatcat ggaactgacc accacgaccc cgttgcacga tatcggtaaa
     4621 gtaggggtgc ccgacagcat tcttttgaaa ccggggaaat tgaccccgga agaatacgaa
     4681 gaaatgaaaa aacatgtcgg atacggagtg actgccctga aaagcaatct cgatccgacg
     4741 gcagagacac cgagttttat caatactgcg cttgagatta ttaactatca ccatgaaaaa
     4801 tacgacggca gtggctatcc gaacaaaacg aaagggaagg aaattccttt gcccggccgt
     4861 ctgatggcga tcatagacgt atacgacgct ctcataagca aacgggtata caaggaagcc
     4921 ttttcgtttg aagaagcgaa cgcgattatc agaaaagata ctggaagtca ctttgatccc
     4981 gacatcgccg aagcttttct ggaaataaca tccgaaattg aaaagatcgt cttgccatat
     5041 atgaattaaa aaagccgaca aggaaggaaa aacaatgaga aaaattctac ttgttttcat
     5101 gcttttactt tcatcatccc tgacatttat taatctgtcg gcagccacaa cggaaaacga
     5161 acccattgtc tggaccaccg aggaagccgc atttctagcg gatcatccag agatatccgt
     5221 cggcatcgac accgaatccg ccccttttga attcattgag aacgatcagt acaagggact
     5281 tactgccgat tatctggaaa taatcgccga aaaaaccggg atcgaatttc agatagttta
     5341 taacgacacc tgggaagaaa catacaatca ggccgttgcc ggcgacattg acatgctttc
     5401 cgccgtttcg aaatcgcacg agcgggaaga aatattcctg ttttccgaga tgtattacga
     5461 gcttaggcgc gtgatcgttg taatggaaag caacaccgac attcacggca tcgaagatct
     5521 ttacgggaaa acggtcgctg ttcatgccgg cagttcgcat tacgattttc tgctcgatta
     5581 tccggagata accatcagtt cttattctac ggtgttggct gccatcaccg atgtttatca
     5641 gggatatgcg gtggcttata tcggtaattt ggccacaagc gactacctga taaaatccca
     5701 cgggatttcc ggcctcagat atgtgaatct ggaagaggaa aatccagcgg gattgtattt
     5761 tgcctttcat caggattgga cggtatttgc cgggattatc gacaaagtct tgttatcgat
     5821 cagcgctgaa gagaaaatcc agatcaattc acgttggatg acaatcgact atacgactga
     5881 ctacggcccg cttcttaaag cgatcggaat cgcttcggcg attttgttac tcggcggcct
     5941 tttgtccttt ttctggaata tcaaactcaa aaaggaaatc gacatacgga aacggactca
     6001 agtcgaactt gaaagatcga agcaggaagc gattgaagcc aacaacataa aatcgagttt
     6061 tctcgcccgg atgtcccatg agataagaac ccctctcaat gcgatatccg gaatggcgta
     6121 tcttctcaag gaaactgatg tgtcgaccac tcaaatgatg tatgcggaaa ggatcacaca
     6181 ggcatccgac acgatgctgg gaatcgtcaa tgacattctt gattattcca aaatcgaagc
     6241 gggtaaagct tcgatcgatc taatatcgtt caatttggac catattattc aaaacattat
     6301 cagcatcata tcggtgaaaa taaccgagaa gaagttgaga ttcagatttc ataaagatcc
     6361 cggaatcccc gtgtggctgg agggcgattg caagcatttt cagcaaatcc tgatcaatat
     6421 cctcaccaat gcggtcaagt tcacggagac cggtgaagtc agtctgacaa ttaccgagaa
     6481 agccagaaac gatagcatta taacgctcag attttctgtc aaggataccg gcatcggaat
     6541 gtccaaagca accctggaaa atcttttcac gccctttact caggccgatt cgagcataac
     6601 caggaggttt ggaggcaccg gtctcggatt atcgatcgcc agaaacctgg cagaagcgat
     6661 ggatggaaag atcgaagtgg aaagtttgga aagcgccggg acactcttta cggtgacact
     6721 tcctttcacg atcgatattg cgaaaaacaa agaaacgaaa acagaaaaat cggaacaata
     6781 cctgaaaaaa ttaaacgtgc tgatgctttt ggctgccaaa tccgatatgg atatcatgga
     6841 aagctatctg acatctttcg gcattgaagc aacaagtaca atatcaccag aagaagcgat
     6901 gcatttagtc gtgaacgaac cgggaaaata cgatctttta atcatcgatt acgaagcccc
     6961 gtccggtcac ggattcagtt atctgaaaca attgcgggaa acagtaaaaa tatctgagtt
     7021 ccccaaagtg atcatgttgc tgccgatgtc acgatcggat ttatacaacc atctcgaagt
     7081 caattttgtc aatctgggta taggaaaacc ggtaatacca tcggttctct ataacgggat
     7141 cgtggatatt ttcatcaatt cccagatcaa ggaaaaggga aagcagttca agaatgattt
     7201 gaaactcgaa tctactggtt acgccaaacc gctcgttctg gtggtcgaag acaaccagac
     7261 taaccagatg attgccagat tgcttctgga gaaatccggg tatcttgtcg tttcggccga
     7321 aaacggacgc gaaggcgtgg cggcatatgc ggccaaccgc gacttgatca gacttgtatt
     7381 gatggatctc catatgcccg tcatggacgg ttttagcgcc gccaaactga tcaagcagga
     7441 ggatccggat attcctatcg ttgcgatgac agccgaagtc ataccgggag taagggagaa
     7501 atgcgaagca aacgggatag atcattatat atccaaaccg ttcgatccgg aaaaattcat
     7561 tgaaaccgtc aacaatatct taggcataag caacttttcc aaagccaaga aagccttgca
     7621 tatcaacaag gagaagggct tgaaactaac cggaaacaac gagaaccttt accagttgat
     7681 tttgaaaact tacagggaag aaaacatcga caccgggcaa aggctcaggg aagtcattgc
     7741 caaacaagac tattccgctg ccagacaaat cctgcacaag atcaaaggca gtaccggttc
     7801 gatcggtgcc gacaaacttt tccagatcat taccgatctg cagaaagcga tcattgacaa
     7861 tgacgaagtg atgataaccg atctgtcgac gctttttgct gacgcaatcc aagtcgttct
     7921 ggcggaaatc gccgagaaca cgtccaaata ataataactg ctcggttttc cgctgttaga
     7981 aaattcgaga aaggagcgtg acgctcatgc aaagaaagat tctttcctta ttcctgctgg
     8041 gctttctttt tgtcatgcct tggcgtgtcg aagcttcgga ttcgactatc gaatggactc
     8101 aggaggaaac ggaattcatc gccagtcatc cggttgtgag aatcggagtc gatccggaat
     8161 tcgttccgtt tgaatttatc gataacggcg aatataaggg gatcgcatcc gactacgtgt
     8221 ctataatcga ggaaaaaaca ggaatcgact tccaggtcac gcccggcttg tcctggccga
     8281 aagcctacga tgaagcggta gaagggaaca ttgacgtcct gccggccatt tcgaagactc
     8341 cggcgagaga agaaatcttt cttttcagcg aagcctatta tgaagtcaga cgggtcatcg
     8401 tcacaaggaa tgacaacaca tcgatcaaag acttggatga catgtacgga aaaatcgttg
     8461 ccgtccagat caattcgtca catcatactt ttcttttgca atatccggag ataagtctca
     8521 gtctgtataa taccgtcaat gacgccattg ccgcggtttc cgcgggaacc gaggaagtat
     8581 ttgtaggaaa tctggccact tccgtataca ttgcggaatc gtttggaata tccaatctga
     8641 aattctttgt gttgtcgagc gaagaaccgg tcgaattgca tttcgctatt cgatcggact
     8701 ggccggtcct tgtggatatt atcgacaagg ccctcgaatc gattacaatc gccgaaaaac
     8761 aggaaatcag ttcggaatgg attacgatcg acatcactcc ggactacggc ccgtttatca
     8821 agtctgtcgc aatcggagcc gcaatccttt tgaccggatt gctgatttcg gtattctgga
     8881 cggtgaaact gaaaaaggaa gtcgctataa gggaaaaaac ccagaaggaa ctggagacgg
     8941 caaagcgcga tgcggaaaac gcaaacaacg tgaaatcggg atttcttgcc agaatgtcgc
     9001 acgagataag aactccgtta aatgctatat cgggaatgtc ctacattctg gagaaagatt
     9061 gtcagacgcc gacgcagggc atgtatgcca aacggatatc ccaggcgtcg gaagcgatgc
     9121 tcgggatcat caacgacatt ctcgactatt cgaagatcga agcgggaaaa acgtcaatag
     9181 aagtcatatc gttcaatctt gaccaagtga tccagaacac cataagcatc gtctcagtcc
     9241 ggatttccga gaaaaaactt ggattcagat ttaccaaggg taatggagtt ccggtatggc
     9301 ttttcgggga tccgaaaaga ctggaacaga ttttgatcaa tattctgaat aatgccgtaa
     9361 aattcacaga caaaggcgag gtgcggctga atatcgatgt gaaatcgaat gacaacggta
     9421 taataacgat ctgttttacg attgcggaca ccggaatcgg catgtctaaa gaaacgcttg
     9481 acaatctgtt cgttcccttt gttcaagccg attcgagcat cacgagacga ttcggtggta
     9541 ccgggcttgg tttgtcgatc gccaagaatc tggcggagct gatgggcggt atcatattga
     9601 cagaaagcgt ggaaggactc ggaaccactt tcaagatcag tcttcctttc gaagttgacg
     9661 tgatgaaaga ggaaaacaga tgtaaagagg aaatcaacca ggaaataaaa aatctgaata
     9721 tcctggtttt agatgacaat ccctatgatc tggatatcat cgccagctat ctgtcggcat
     9781 tctgtcttag ggcacaaacg gccggttcgg tttatgaagc caagcaaatc cttgaagacg
     9841 gagccaaaaa aggcggaaac caaatcgata tactgattgt tgattacaat ctcaaattgg
     9901 aagacggttt tgcatttgtc aggtcgctta acaatgaaac catgaaagac aagatgccaa
     9961 agatcatcat gatgtttccc tttgacagac cggaactttt cgacctgatg ggaaaaagcg
    10021 gtatttccga aggaatcgac aaaccgatca ttccctcggt actgttgaat aagatcatgc
    10081 aactggcttc tttgggaaaa tcttcggcag ataacgagaa acacgatgaa acattcgcaa
    10141 tcccaaaaga aaaagcattg attcttatgg ctgacgacaa cgagaccaac cggatgatca
    10201 caaagattct cttggaacag gatggttaca gggttatagc cgttgaaaac ggtaaactgg
    10261 caacggaagc gttcgccgaa gaaggcaaaa acatcagctt ggtattgatg gatctcaaca
    10321 tgcccgtcat ggacggatgc gaggcatccg acctgattat tcagatgtcg cccttcattc
    10381 ccattgtcgc cataacagcc gaagccactc cgggggtgcg ggagaaatgc cgagagcacg
    10441 gggttttcca ttacatatcc aaaccatttg atcccaaaac attcccgtct tttgtcgata
    10501 aaataatcga taataatata actagatcaa tggaaccgca aatcgacaaa gagtcaggcc
    10561 tggcccgtaa taaaggcgac atggaagcct acttggattc attgaaaaga tttgcgaatg
    10621 agaacgtgaa tgcattcgag gacttgtggc atgcgatcga aaaaagcgac tgtcaaaaag
    10681 cggaaagtgc cgttcatagg gtttcggaag gcgctacgct gatcggagcc gaaaaagtcg
    10741 gaccgacatg taaggaaata ttgaatgaaa taagcgtccg caatcgcagc gaagtgataa
    10801 gactggcaga aaagttcgag tggcaactgg aaatgattat gcgggaaatc atatcggaac
    10861 tgagaaaaaa ataatcaaaa aaaaggcggt cattggtaga ccgcctttgt tattacggct
    10921 gaacataaag tgttttgaga taatccgaca tgtcgttgta tcccagctgg taaagttctt
    10981 tcacatggtc caccgtaaaa tcaaggggac ttccgatcga tgttttgtgg atgatatcgt
    11041 aaaaccggat tcccggataa agttcgggat gatagggcgc gcggggattc aaacggataa
    11101 tgatgatcgt gtcgcatccg gcttccgcca gtggcttgat cggaaccccg tcgtaaaccc
    11161 cgccatcata gtattttttg tcatccgctg caataaccgc tggaaaaata acgggaatcg
    11221 aacacgaagc cacgaccgct ttgagcctct cgttgtcgga catctccctt aacggcagat
    11281 agaaagcctt ggaatcgtcg tgtaaataac gcctgaagcc cattctcaga aaagcagcga
    11341 atccgctgtc ctcactttca agttccgata ccgtgacata tacttcacgt tcgttcaacc
    11401 gtttctgatc gacacttgcc agaagaagat cttccagagg atcgatcttg aaaagcccgt
    11461 gatcgatcat gttgaatttt tctttgagcg aagaagcgtg tccacgagtt ctatttcttt
    11521 cgagggcatt atctggcata ctcagccaga gatctctggc tgcttcaact gatgtggaag
    11581 ccaaaagaca gacattggcg gctccgatgg aagtgccgga aaaagcgtcg atccgatcga
    11641 aaattccctg ttctttcaaa accatagcgg ccccgatctg gtaagatcct ttggcgccgc
    11701 ctcccgacag gcagagtccg agttttccca ttgtaacaac ctccattttc gatcatctat
    11761 tatattatat cataaaaagc atcggtgcaa atgaccttgg caaaaccgtt tgacaaaaat
    11821 aaatgttacc gccccgggct gtctatgtta taattataac atcgaaaaat caatatgaac
    11881 gggatgattc acatgggatt gacggttttt ctgtttatac ttgcatatct cttgttaaat
    11941 ttatggaatg cggtagtttt gacccatcaa aaattgaatc gctatatcgt acctttccgc
    12001 cacacacttc tgggcgaact taacgcaata ctggcaaata cggccgtatt gggccttttc
    12061 ctcatgatag ttcttgtatt cacgaagaat gcatatgctt tatgtctcgt gcttgtgatc
    12121 ctgacgggag tcttcaacac ctattttctc ggacttaata ttttcaacct gtattacggc
    12181 acatcgtttt ctagagaaga cttggaaata ttcagaaatc ccggcgaagg catcgccaag
    12241 ggtatgggcg gggaactgat aagggaattg ttcgtttatt atcggatact cctatttctg
    12301 ccttttgctt ttctgctggt attctttctc aagactgatc cgatcctgtt atcggcttat
    12361 cgcgtcccga taagcaacgg agcggttttc acgatgatcg ccatatttat tctggttttg
    12421 tttttgaccg gtactttatt cgaaaaaatg tatgccaaac aattgatgat ccaggttctg
    12481 aaaagcacct acgcaatcca aaacttcgga gtgtatccat attatttgtg caattttacc
    12541 gggatcacca agttgatcga cgccaagata gaaaagcgtt ccggaccacc atcagacctt
    12601 ctgcccactt tccagaaata caataaaaac aagaaaaaat atgtaaacct gttagatggc
    12661 aaaacctatt ccaacagtat gtcgacagac gatctaagcg agaacatttt tgtcgaccct
    12721 tcgcttataa aagaagacaa aagtttagat ggaattctta aaggacgcaa tctcgtcctt
    12781 gtccagatgg aatcaatgaa cagatatctt cttgacatcg gactcacaaa agacgagttt
    12841 tcgtttctca aagccctgct gcctgaagcg ctcgatctcc gggaattcta cacaagtgtc
    12901 ggcatcggcg tcagttcgga tgccgaagtg acaaccctga caggattgtg gccgaccgga
    12961 cgcagcaatc tttactgggc ggattacgac agaaaaacgc agaaatattt ggttaaacgc
    13021 gatctttcga ccattgtcaa atatttcaaa caagataact attccaccaa ggccctgcac
    13081 ggggactaca aacgcttcta taaccgcgag cattcatacg gtgagatcat cgggtttgac
    13141 gaattccagg ccatggagga tttcacggag ataaaagagt gcgaaaaaac cggtatattg
    13201 aaactgtatt catatgaata tgccgatgga aaagagcatg tctctccctg ggtcagcgat
    13261 tatctcctag ccgaaaaagt aagagaaaac atccacgaaa aacaggggcc gatgctttta
    13321 tatccgataa cgatgatgcc gcacattcca ttcgagttct atcccgggga aatgaataag
    13381 gcattaaaag attcccagtt gaaagagcag acgaagaagt atctccgttt tgcggattat
    13441 tatgacgatt tcatccgccg gtttttcttt acccgcgacg ggcagattga caccgacccg
    13501 aatactgtct acatttttta tggtgaccac ggatcgggaa tcaaaaacgg cgatatcgaa
    13561 aaattggttg accggaaact gacaaaaatg gaagaaagaa agattcttat ccaaattgcc
    13621 ggtttcatct atgtcccggg aagcgatttt gtcgaaagaa acggcatcag aatccgtaaa
    13681 ggactgctca aaggccatca ggatctcgtc aggggacaaa tggaccttaa ccggacaatc
    13741 atcgacctat tcggcctgaa tggggaaaaa gacgcctatt tcggcgtcag ccttctttcc
    13801 aaggaaccga ctttcatcct cgataacaaa ttgcaggatg tcgtgaccga caaagtgatg
    13861 ttttccatgc ggaataccga gaacactttt ccctccggcg tcgagattgg gaaaggaatg
    13921 ttcgatatcg tgaaggaatt caaattaatg aacgaacggc ttttacaaaa accggaatgc
    13981 gaaaaaaagc ttaacgaaag aatgcacgca agcggatcgg aatgatccgc ttttttcatt
    14041 actccatgat aacatcatgt gaattttttc ctttttgtgt tttccaaaac atagttttga
    14101 tgtatgatat tattatgttt caaaacgata aatcgtttaa cggagggaat catgactttc
    14161 aaaaacatat ccagacgcgt tctgattgtt attctgtcaa cattttatgt cctgatcacc
    14221 ctgttcaacg tgattgtcct gttcttttgt tccttgcggt tcactgacaa caggcaacag
    14281 ataaatcatc tggacgatct tcaggaatat tacgaaacaa ccccgtcatc cgaaatcgct
    14341 gccgaacagt cctttgtttc tttcgacatt gactttaatg atttacggtt gaacgaaatc
    14401 aagatggtgg cgtcgcacaa ctcctacaag aaacagggaa cggcgcttgg aagagcgatg
    14461 atcgctcttg ggtcgtctaa ggaagacgcc agacttatga actacgggaa cgtctggata
    14521 tccgaacagc tggatgccgg aattcgcagc atcgaaatcg atcttcgtta taaaaacggc
    14581 agttttgtca cgacgcacgt gcctttggtc gacaaccgct ccgtttgtcc cgatttctcc
    14641 cttttgctgg aggaattgaa gctatggtcg gacaaccatc ccaatcacat tccgatcatt
    14701 atcattttgg aactgaagag cgattggatg gtgttggatc ccacacttga cgatatcgga
    14761 caaagcgaac tagaaaggct taacgacatg ttgttgttca atttcgattc ggcgatttat
    14821 accccggaaa tgctcaaagg gtcctatgat tccttgcaag aaagaatcgc aaccgaatca
    14881 tggccttatg tctctgactt gttgggaaaa ttcatcttcg tactgcaccc cggagaattc
    14941 accgacacat atgtatcatt gtttccagat tatgagggaa tgagcctgtt cccggccgtc
    15001 agcaatcttg acaccgatca tggttacgcc gctttcgttg tccataatga tccgaatatc
    15061 gaagaaataa acgcgcttgt cgcgcaaaac tatattgtca ggaccagaat ggacgaagac
    15121 ttggtgaacg atcccgacag gatggccgat gccatcgcct cgggagccca gatcctgacg
    15181 accgatctcc atccggcaac ccagattcag aatgcgaccc cgatttattt ggacggaaat
    15241 tacaccgtca tcgtcaatga cattacccat gaggacgaat gacggcttat caaccgaaag
    15301 gtggtttgtt aaaatgtatt cggaaacttc ggcacaaaga ataaaagaag atatcttcct
    15361 gatagacaat tcgctcaaag aacgtgttcc atcgcaggct cggagcttac tttccattct
    15421 gatgaggaca tatgacggaa tcttaatgag tttgaacacc gcgatgcttc cgacggaaag
    15481 tccgatctac ggacataatt tcgacgaact caagaattac gacgaaaatg ttttttccaa
    15541 cctgaccgta atcaggaaca aactgcaact tttcgatctg caatttcaga atgttctcga
    15601 aaagaacctg gaagccggca atacgggata tttgtcccaa aatgagatcg tcaatgcgat
    15661 caaagtttgc aagaacgtcg atgccaaaga gaaagaagat gccctgaaaa tggcggaaat
    15721 tctcaaagac atcgccgaag gggataatct tggcttccag gccaaatggg agcggatcaa
    15781 gccggttttt gcctatttgg cggatgccaa tccgaatctc ggatcattgg ttatggaatt
    15841 attgaaacgc atggcatgac cgatattttt taaaaaaagg agaaaacatg gctgatttta
    15901 catcgcgact gaaaaaatac attcagctgt gtccgatgac atcgggcctt ttgactgtca
    15961 atacagcttt tttaatcatc acgatcatca ccggagggtc ggaagcgttt ggcagcgaca
    16021 acctggtcag gctcggtggc atcgtgcctt cttacgtggc ttcaggtgaa tattaccgca
    16081 ttatctttgc gatgtttttg cacggcggtt accttcattt cttttccaac atgctggcgc
    16141 tgtattttct tggcacaccg atggaaagaa tcatgggcgg atggcgttat ctcgctttgt
    16201 acatgttatc cggaatcggt gccggcatca tgatcgtctt gttcggttat tcgaacatca
    16261 catttctcaa tacctacggc gaagtttcca tcggagcgag cacggcgata tacggaatta
    16321 tggccggact cgtattcatc acgataaaaa ggccggggtg gtttccccgc caaacggtca
    16381 aatcaatttg gatgatcgcc gttttgaatt ttatcatcac tttcattttt ccgtatattt
    16441 cggtagtcgg tcatctttcc ggttttgtct tcggctttgt gctgagcctt gtattgatgc
    16501 ccaagattcc ggaatatcag agaaaagcag tcattgccaa gtacttccag aagaaggcaa
    16561 caagcgaaaa cgataacgac gatgatgatt atcattactg agaattcgaa ttaattcgaa
    16621 ttttccaata ataaccggct tcaaaaacgt atattaaaag atagaaggtg aaaaaagtca
    16681 tgacaaaaaa acattacacc ttgctgaatc tggctatttt catccccatg ggcttggctt
    16741 tgctgtcctt tggctttgca atctatttcc agtcaaacgg atacgccaag gaaatctggg
    16801 ttcctttggc tttgtcagtg ttctgcttcc tggcggtcag cttgattttt ctatttgtca
    16861 attacccggc actcatcgaa taccgtacga agaaaaagta tgaggaactc aaaaagcgtc
    16921 ccttatgttt cgcttatcag agcgaaacaa ttgaatcatt gacaagacgt ctcggaaaac
    16981 tcggatttta tccggacaag aacggtatgc tttataacaa gataccctac cttctcaccg
    17041 gatcgatccg gttttatatc gcaatcgtcg acgctgatga gaacccggcg tatttcgaga
    17101 atatcatcgc gaaatatcac agcgatccga tatatcaatc cgaaaaggga taccgatgcc
    17161 tgacggtgat cggtatgaaa aaaaagctgt ccagaaccga tgcggccaaa atcaagaaat
    17221 acaccgacga acatctgacg ctcgattccg ctttgtttgg cagttccgcg gaggcatctt
    17281 tttggatgct gtatgaatcg ggaaaaggaa gattcctttt caagaatgtc ggaaacggat
    17341 cgaagatcaa agccgctttc atcggaggct ggatgctgaa gaaattattg ttgctcaata
    17401 atacggaaat cgtttgcgaa accgacgacc cgttcgatag tacgcttttg tcaccggaaa
    17461 aataacataa tactataaaa aaagacgttc cctcgggaac gccttttttg tcatcagacc
    17521 gtttgtcttt tccagtcgaa atagaagaga atgccgacca ctatgaacgg agcagcaacg
    17581 atgatgccgc ttatcagcat ttcgatattt ctaattgata aaaaggtgat ccaagcaatt
    17641 ccggcaagag gaaacaaaat agcccccaca atctcccatt tccacgtaag aatgaaaatc
    17701 agaatcaaaa ccgcggtggg aatattgtgg atcaggaatc cttgcatatc attccaaaac
    17761 ccgtttcccg ttccgaacga atcgaacgaa gttgcgaaaa ccagtccgat ggcaatcact
    17821 gtaaataagc gtggaagcca aaacagccaa tcgtgttttt tcatattatc ccctcccgcc
    17881 ctcattataa caaattaaat tattcttgca agctccaaag aaaaatatat tattttaatg
    17941 aaagcgcttg atatttaccg aataaacgga ttataatcat tttgtggtaa taccacgaaa
    18001 ccgaaaggag atttcgccaa tggataaaag cgccggaatc aaacaaccga agaatctaac
    18061 aaaaagaatc gaatggttgc ggaattacta cttcgaaggc gtcaaacgcg actggaacaa
    18121 tgaatatcat tgctatacaa ccggaaagcc atgggacttt ctattcgatg agataacata
    18181 ctatatcgtt ccagagacaa ttccttttta ccagacattt gcctccagtt cgcttcaaag
    18241 tgccaagacg atcgcattgc cggacgattt ctggaaattg tccctcaagg aaagaagagc
    18301 aatattcatg aaagaagctg tcgtcaataa tatgccggcg gacttactga agggcgattt
    18361 gatttgcggt gcttgtttca atttgctgac atcgcattgc ctgaccaaaa aggaagccaa
    18421 agaccgtaat catctggtat acggcaaaaa tggttcccgc aatacggtac ttgactacta
    18481 ttactacggt ttcggaaaca ccggatccac ctgcggacat ctgattcctg attatgccaa
    18541 gatcgtaaaa aaaggattca gagccgtgaa agaggaaatc gagaacgaat acgattcgct
    18601 ttctgaaaag aagaagaaat cctccgaagg agaacaactc cgggcaatga tgatcgcctg
    18661 tgaaatgcca agggaactgg cagccaaata tagggaaaaa tgtttgaatg aagcgaaaac
    18721 agaaaaggat gaaagtcgta aaaaagaatt ggaactgatg gcggaaaatc ttcttgtcgt
    18781 accttgggaa ggcgcaaagg acttctacca ggccatccag tcactctgga ttacgcatat
    18841 gctggtgatg tcggacgaaa actatcccgg accgggagta tcgttcgggc gcctcgacca
    18901 atatctgtat ccatattacg agaaatcccg taagaacggg atgtcggatg atttcatgaa
    18961 agagattctg gaatgcttct ggttccattg caatactgcc tatgacgccc agatccgcgt
    19021 cggaaataac ggtatcaccg ccggattcgg acaactattc aacatatccg gttgtgataa
    19081 ggacggaaac gacctgtcga atgatctgac ttatctgttg ctagacgtga tcgacgacat
    19141 gtcgccgatt ttagagccga aaccgaacgt gaggctgcac agaaattctc ccgaaaaact
    19201 tttggatcga gtcgtcgaca tggtggtatc atcgcagggg gctcccttcc tgatcaattt
    19261 cgatgaacgt tcgatggccg gaatgatgcg tgaaggacgg atatgccacc ttgatgatta
    19321 catcaatcta aacaatgtca gcgattacgc ttcggtcggc tgcctcgaaa acacaatgca
    19381 gggcaatgac agaagcggaa ccgtcgataa taatctttac ttctacaaag ccatcgaatt
    19441 ggcgttaaac gccggcaagg atttgacacc taaacataac gatacagacg gaagaacgct
    19501 tccgatccgt caaaacgggg tatataccga caaggtagag gatctcggat cgtttgccga
    19561 tttctacgaa gctgtcaaac gccagactgc ccacatcatc cgaaaaagca cagaagtcta
    19621 tgaaaaatcc gaaaaaatcc gatgcaaata cggaaacact ccgtatcttt cgaccctggt
    19681 tgacggtacc atacaatcgc ataaggatgt cacggaaggg ggagcagagc tccggttcgt
    19741 gacgatcgaa ggcgtgacat ttgccacaac aatcgattcg atccttgcgg ttaaatatct
    19801 ggtctatgac aaaaaagaat gtgatctcaa gactttgaac gaggctctta aaaacaattg
    19861 ggaaggctat gagattttgc gtcaaaaagc cataaataag gctcccaaat acggaagaaa
    19921 tgatgaagag gccgatgaga tcgccaaaga ctatatgaat ttttttgccg aagaaacctg
    19981 gaaacataag accaaatcga caaaccgtca atttcgtccc ggtatgcttt catggaatta
    20041 ctgggcatcg gcgggattca tcctgccggc atctcccgac ggaagaaaac aaggcgaatt
    20101 cttctcaaac gctatttgtc cggtaaacgg ccgcgatatc ttcggcccaa ccgctaactc
    20161 caacagtgta ggcattgccc ttggcggaaa agcggaagca ggcgacttca aggattatgt
    20221 caactatctt ccgaatggcg ccagtcacac cataacgttc aattcgtcta tgctggccaa
    20281 tccggatcat aaggagaaat tcaaatcgtt ccttagaggt tatatcgaaa acggaggcac
    20341 ggcgcttcaa ataaacattc tcgatccgga aatgcttaaa gacgctcaga aacatccgga
    20401 aaattacaaa catctgctag tcagggtcac tgggtacaat gcatactttg taaccgtggg
    20461 aagggaactt cagaacgaga tcatcgcccg cgaatcccac aaagagtatt aggcatgcgt
    20521 attataaaca tccaaaggat gtcgaccgaa gacggtcccg gacttcggac cacggtgttt
    20581 ttcaaagggt gcccgctttc ctgcaaatgg tgtcataatc cggagtcgct ctcttacaaa
    20641 attggcaaag aatggattgc ccaatattgc atgttttgtg gaacctgtgt ggcaaaatgt
    20701 ccggaaaatg ctctgacaat aacaaacaat gaagtaagga tcgattccga aaaatgcaaa
    20761 atgtgtctgg aatgcgtcga aaattgtccg accggagcaa tgaaatcgct cggtaaagat
    20821 atcggaattg aagaactgtt ccgggaactt atcaaggaca aagcctattt tcgggctacc
    20881 ggcggcgtta ctttttccgg cggcgagtgt ctcttgcaaa cggatgaatt ggtcgaactg
    20941 gcaaaaatgc taaaaaaaga gaatatcagc atcgccatag atacttccgg agcaatcaat
    21001 taccatctga tcgaaaaaat attgccgtat acagatatct ttctttacga tatcaaattg
    21061 atcgacaagg ccaagcacaa ggaactttgc ggaaaagaca accaagaaat tctcagtaac
    21121 ctgatcgccc tttcaaaggc gaaagccgat atctggttaa gaaccccaat cattccgcgg
    21181 gcgacggcta caaatgacaa cataagggcg atcgccgggt ttttaaggaa taatcatatc
    21241 ggtttttccc gctgggaact ttgttccttc aacaaccttt gcaaaagcaa atacgaaaga
    21301 atgtccatcg actgggaata taaggatgac cggttgatga cgaatgagga aatggaagaa
    21361 ctcctggctt cggccaggca ggaatacgga aatcccgaag ccacgatact ggcaaccgga
    21421 tcccgaaaaa cggaggaata gccatgaatg acaaactgac tcccgaaatg atcgaagaat
    21481 ttgccaaatc cgtgaaagtc ggattggtcg catcaatcga tgagaagaac tccccgcata
    21541 tcaccgtttt gtccactctg atgggaaaag gagaaaatac gatggtgttc ggaaagttca
    21601 tcgaaggatt atcgaaagaa tttatccaag aacgtccacg cgccggtttc cttatcatga
    21661 atccggaaaa ggaattctgg tatgggaaaa tgatttatga aagcgagaaa aaagagggag
    21721 aagaatacgt tttctacaac aaccagcccc tatatcgcta caacacttat ttcggcatca
    21781 atacggtcta ttattccaaa ctggtatgta tcagcgacat ggatactctc ccgctgggaa
    21841 aaatcattgc cagttcgctt tccgttatgc taaacaagac aaggtataaa ggttccgcca
    21901 agaccgaagt catgaaacca tgggcgaaaa aattcaccgc aaaactggat accctgatgt
    21961 ttttgtccta tatggacatt gagggctatc cagtcatcgt tcccataatc caagcccagt
    22021 cggcttccac agatcggatc gtttggaaga acaagccgtt cggagaaaga cagctttccc
    22081 tgaaaccggg acagaagatc gccattctcg ggttttcaat gtcgatggaa accgtcttgc
    22141 tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggacaga agatcgccat
    22261 tctcgggttt tcaatgtcga tggaaaccgt cttgctcaaa ggagaatttt ccggattcga
    22321 caagcgaggg ttcggatatt tggcgatcga acgggtatat aactcgatgc caccggtaca
    22381 caaatacgtc tacgaagaga atgaagcatg ctgaaaaatg ccagcaagac ggaaaaagtg
    22441 gtggaaagta tccgtaaccg gataatatcc ggcgaactaa taccgggcga caaactcccg
    22501 ccgttaagag aactggtttc gatatataac gcaagcagga gcgtcatcaa ccaggcaatc
    22561 ggaacactta gcgccaaagg ctatctcgaa gtgaaaccaa gacaatacat catggttaac
    22621 gatatttggg cttccggcaa tctggagatt gtcaacgacg tgtacttcgc tcccaaaagc
    22681 cgttataaat ccagggcgat tagagaaatg ctcaaagcga gaaaaatggc ggaaatggct
    22741 tcgcttggcg aaataacacc tgacaatatt ccaaatcttt cacaactgaa aataatatat
    22801 gccgaagaaa aaaaatggct tggacagaaa tcggctgata tcgggatcat tgccgatctg
    22861 gactcgaaat ttcatgaaga agtggtgagt ctttcaaaca atttcgtgct ttcgctcttg
    22921 tatcttgctt ttaagaatat cggggatgat ctgatcagaa aattctattt ctgcgtcaac
    22981 gaaacagatt gtccgatcaa ctggcacggg caattcatcg aagcgttgga aaataaagat
    23041 atcggtaatg ctaaaataat ctggcttaag cttctttcca agggggaaga agtgatattg
    23101 caaaacatcg actgaaaagc agatcggcaa cgatctgttt ttttaattat ctatgtaaag
    23161 catacttgac attaatcgat tcatgtcata tgataagggc ggagatgaga aaagatgatt
    23221 ttgggaaata atcttcggcg gcttcgtttt gaaaatggcg aaatgtcgca acaggattta
    23281 gcggatctag taggcgtttc aagaatgacg atctattcga tcgagaaact gaaatacact
    23341 ccctccgcat cgctggcttt gaaaatcgcc aatgtgttca agtgtccctt cgacaaagtt
    23401 ttctttttta aaactaaaac tatcggggag gatgaatgag atgacttcga gacagataaa
    23461 attagtcata ttcgggggcg tcacgcttct ttgtttcgtt tatgtggcgg tggctttcgc
    23521 gatcatcaaa aatcaactgg ttccttcgct tgccgaacca actttgctga taaactattt
    23581 atcatacggc atcatgatcg ccttcgtcgt tttcggcgtc tattgtcttc tgatgctgag
    23641 ggatgccctg atccagatcg tccagatccg ttcgggaatc atatatccgt ctttacttgt
    23701 agttttgacg gttttgggcg gagtggcgat tttagcggat atcacattgc tttctgatat
    23761 cggaaaggaa tatcttcagt tcgatgtcgc tggagaatgg aaaatgcttt ttggcatctg
    23821 ggctttccaa accgttacca tcatttttca aattgtcatg tcctgttgtc atgatgtagc
    23881 cggcaacaag ctgacggaaa agcttcagca catgagcgat tccctctatg tttcaacctt
    23941 tcaggtgatg ctgatatcct cgatattggg actgatatcc attttcacga cgtttttctg
    24001 ggcgaacaga ctaccggagt tgtcaaaata tgctaccggt ctgatgatcg tcctttgcat
    24061 tttgagcgtt ttgccggcgg ctgtaatcat ctgtcatctc attatcagga atcgccgccg
    24121 cggtttcaac gactggttcg acgaaaggca atgggacaat ctgtcgaaag gcggaatggt
    24181 caccgtgatt accggtgtat gtttcctgat cctttcgatc atcgtttccc agcttgccag
    24241 cggcatcgat cccggttatc tgttattgac tgtgttattt gccgagatcg gcgtgttttc
    24301 cgcttttgtg ctcagccgta atcgtattga tgactgaatc ccggatgaac cgggattttt
    24361 ttatgaaaga aaggttattg ccataagctt tttattgtat aattatggta gagtcaaacg
    24421 ataaaggaat ggcttttttt atgaaaaaat actttatgta tttttggaaa gacaaagtat
    24481 tcgtcatcac ttttgttttt accgcgattt catgtttttt catcactccg tcgatggaat
    24541 atctggatta tgtggattgg aaagtgccat tcatcatgtt ttctctgatg gttgccgtgg
    24601 ctggtatgta cgaggccgac cttttcggat atatgagtat aaaaatggtt ttgcggtttc
    24661 atactttgcg ttggatcgca ttgtcaatca ttatggcatc tttttttctc ggcatgttgg
    24721 tgacgaatga cgcggtgctt ctgacctttg ttcctttcac cattcttgtt accaaacaga
    24781 ctgggcagga aaaatatgct ataacgatta tcgctctcca gacaattgcc gctaatctcg
    24841 gatcagccct gacgccgatg ggtgatccgc aaaatatcta tctgtatgcc tattatggta
    24901 tccgatttgg agattttctc agagtgatgg cgccggtggc cattggcgga ttttttattc
    24961 ttataacaat gacaggcgct tttgttccca atattttttc cgagccagta atgatagcgc
    25021 cgaagatcaa ccgaaaaaaa actatcgtcg ctctgatgat ttttctgaat gctttgcttt
    25081 gtattttgcg tgtgtattcg gaatggatag ccatcatagt aactgttgtt atggttttga
    25141 tatttgatcg caaactgtta aaaaaaccgg attacacctt gatattgaca tttattgctt
    25201 tcttcatttt tacaggcaat cttggtaaaa taccgggaat cgtctcgttc gtatcgggtc
    25261 gtttggagaa tggaacatcg gtctatcttt ccggtttttt cttgagccag tttatttcca
    25321 atgtgccttc atccgtcttg ttggcgacat ttacaaaaca ggagtactgg ccatatcttt
    25381 tacaaggcgt aaatgttggc gctatgggaa cattgatcgc atcgcttgcc agtttgatcg
    25441 ctttaaaatt cgttttgaag gaatttccca gacagggaaa agaatatatt cttacatact
    25501 catttttttg ttttattgca atactagtca tcactatggc tgttttcata tttgatggta
    25561 tatgagtaaa accggggata aaaaaccccg gtcttttttt ttgtaaaaac agtattgtgt
    25621 tatgcacact aatgtgatat catattactg tgcaatacac agaaaggggt ggcatgatga
    25681 attcccaatt caaaaaaggg ataatagaaa tgtgcgtcct cgagttgata agtcatcgcg
    25741 atatgtatgg attcgaagtg attgaacgct tgtccgagga cattgacgtg aacgacaaca
    25801 ccatctatcc cattttgagg cggcttaccg aacagggtta tttcgataat tatgtcgaat
    25861 caactagcat gggagcgccg cgaaaatatt ttcggattac ggaaaaaggt tctggaaaac
    25921 tgactgaata caaaaccgag tgggacaaat ttctcaaagg agtatcaaga ataatggagg
    25981 agaaggcaaa gtgaaaaaag aatatctggc gaaactgaaa cagaaactgg aagaaaaaga
    26041 ttttgataaa agcgaaatta acgacatcat cgacgattac gacaggatgt acgatgatgc
    26101 tttgttgaaa ggaatggaag atgcggacat aatgaatttt cttggcgatc ccgacagaat
    26161 cgctttcgat ctgtgcgatg gaaaaataag aaaacagaac cgaaagaaca acgggaaaat
    26221 cgttgccata atgccgtttg tttctctgat tttatttttt atcatgggat atgcttttca
    26281 tttgtggcat cccggatggc tggttttcct gttgataccc gtgacagcca taattgccgg
    26341 agcgcgcaag gataagaatg gaacactcat tgccctgatg ccttttatcg ctaccgccat
    26401 atacctgggg tttggattcg gcatgaatct ctggcacccg acatggctga ttttcttttt
    26461 gataccggtg acagccattt gcagcggaca aaccagattg cttggcaaac tgaccgcttt
    26521 gacgccattc atcgccgtca ccgcattttt cattctggga gagtacggtt attatcatcc
    26581 cggatggttg gtgtttttcc ttataccgat ggttggcgtc ttgaatgaaa agcatgtatg
    26641 gaaagccgtg atttatgaag catcattcgt catagctatc gctttatatc tcgcgatcgg
    26701 atacttgaat ccgatttggg cacaggcttg gctagttttc ttgcttccgg tcattgtttc
    26761 cttgttgttc ggagaataca acttccaaat agacggcatg tccgtcggca tcagaatact
    26821 cacaattgtg ctgatcgtta tttacgtcct gttcggattt ttgtttcaaa cttggagtta
    26881 tctctggctg atttttctct tgattcccgt tgccgcaatt atcggcaatg gcaagggaat
    26941 gcagaaaaaa cttgtagcac tgagtccttt tgtcgctacc gttgcctttt ttcttctggg
    27001 gtatctcgga ggttactggc agtattcatg gatggcattt ctgttgattc cgatgactgc
    27061 gatcctcacc aaaggaaaag aagaataata ataaaaaagg tccgcttcgg ttgaagcgga
    27121 ccctttatca tacactgctt ccgcccgaaa gaagagacag aagcaaaaca gcatagcata
    27181 agggaatcat caggacaagg taattgacgc aaaaagccat gatggtttcc tttttccatt
    27241 tgctggattt tttatatata aaccatccaa atcccgccgt aagcaataaa acaaaaaaga
    27301 gaaatatcac gccgaaccag taaaaggtgt ttattccgta aaacaagaca tatgccggaa
    27361 caagcaaaga agcgtaaaaa ccgagcggga agacgatttc cattctgaca cgaagagaat
    27421 ggccggaatc gtcaggaaga gcttttgaac ggaaaatcca aaataagata acaaataata
    27481 gcgctcctac gaaaaccaca atgtttaaac ggtttaccaa aaggtttctt tcgcttgaaa
    27541 tgtatcccgg attagaatta tagaatgagt ataggatact aaggcaagcc agtattggaa
    27601 caagaaggaa tcccagtatc aaagaaagcg tcactgactg acggggttta ttcatgggct
    27661 ttatggaaaa tggcatgtat atgagagatg tgagaatagt tccggttgcc aggatataac
    27721 cgatataaaa gaaaacattt cccgatgcta gcgtgtttat ccggctaaga tctgcctgtt
    27781 cgaatccgat ataaaagaaa agaaaggcca cttcatactg tgtatgtatg gttagatcag
    27841 gtctgatgat aagaaaaaga atatagatga aataaccgaa atatgcaatc cccagagcaa
    27901 gaaccgggac caatggcctt tttttcatcc atgacaaaat gataatagga atgccgattc
    27961 caatgacata ataaagaaat ttcaaaaaat aaggaccgac cgaatttgtg aaaacaaacc
    28021 tcagatagac aaatccaagg aatgcgatgg ttacaagcaa agccgaagaa agacgcagcc
    28081 atttatgaga tttcatgaac gcacttcctt tcatgggtta tcttcccatc cattatatta
    28141 cgcaaaaccg aaaatttgtg ttttccagta aaaaagagtt gaaaaaagct cccgcttcat
    28201 ataaaatatt aagtgctaag tcccgcaggt gtagtttaat ggtaaaacgt ctgcttgcca
    28261 tgcagataat gggggttcga ttcccctcgc ttgctccaga aaaaaataac agcgatatcc
    28321 atcgctgttt tttgtttatt cggacttgat tgactgttta taccttttcc ggaaatcggc
    28381 cggggtcata ccgttgattt ttttaaacac ttcggtaaaa taactttgtg aacagaatgc
    28441 caaagaagtg ctgatttcgc ctactgattg atgggtatat cgcaacatgt tttcggattc
    28501 atggattttc atcttcagaa tgcatttctg caaagtcata ccggtctgtt tcgagaagta
    28561 ttg
//
LOCUS       DAOPJL010000016        26264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_1966_length_26264_cov_8.153306, whole genome shotgun sequence.
ACCESSION   DAOPJL010000016 DAOPJL010000000
VERSION     DAOPJL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 26264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26264
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_26264_cov_8.153306"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLP02_03280"
     CDS             <1..540
                     /locus_tag="PLP02_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42506.1"
                     /translation="NKNKRYNGNILNETIVSGNLMRDETNTYKCLNGKGKRMVRKYLE
                     YYVKNLPELVLLRSFISADKKKASFVLLYLNEMNKVIFRWDRSKKIRNLVKGINKYQM
                     LLPTIGFLFEVPMQINEGVPYRMTNHDNGTVISYDVVKDAKHIKRKQKEKPLSKKQIK
                     AQAKIEALKEKRLAKQKKR"
     gene            complement(583..1878)
                     /gene="mtaB"
                     /locus_tag="PLP02_03285"
     CDS             complement(583..1878)
                     /gene="mtaB"
                     /locus_tag="PLP02_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016885482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPF42507.1"
                     /translation="MKKVAFTTLGCKVNSYETEALWEIFRQKGYQRVSDTKAADVYIV
                     NTCLVTNTAAGKSRQMIHRVLKHNPDADILVMGCLSQLEAEKVLAEKNVRAIVGTNDR
                     EKLLGYLDEYHEKGKKINGINRVENRRKFGNLAVSGYSDHQRAFLKIEDGCDNYCSYC
                     IIPYARGPVRSKPLDEVMEEAEILAKSHQEIILTGIHTGGYGKDIEDVTFATLLWKLA
                     GDNNLPRIRISSIEIHELTDEVIEILSGSKRFVHHLHIPLQSGSDKILKLMNRKYDTA
                     YYKDRINKIRKALPDLSVTTDIIVGFPKETEADFVDTLTFAEMIGFNELHVFPYSKRP
                     GTVAAKMTDEIPGNIKKERVGRLIALGEKMAKDAIKAMIGKKLEVIVERRKGDYLTGH
                     SAGYIHVAFKGSDDLIGRKVDVILDREAYPLSVGSLVNK"
     gene            complement(1880..2608)
                     /locus_tag="PLP02_03290"
     CDS             complement(1880..2608)
                     /locus_tag="PLP02_03290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPF42508.1"
                     /translation="MQKYFVLPDKVHDHTCYLSGSDLHHIRDVLRYHTGQQVIIGISG
                     GKSYLGVIGLITRDICEVTLTEELNIIEPYYNVTIAQALIKKDRFELFLEKAAELGVS
                     AVIPTVFSRSVVKIDETKESRKHERFQLILKEASEQSKRKDIPELLPFTILTDIDYPA
                     YDRVIVCYEKNDTDGSLKEIIGQIRPEEKILVIVGPEGGISKEEAEFLSLQKAFFVTL
                     GNRILRSETASIMVLSAFLYEGGR"
     gene            complement(2688..3500)
                     /locus_tag="PLP02_03295"
     CDS             complement(2688..3500)
                     /locus_tag="PLP02_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42509.1"
                     /translation="MAEETIYPILLLHGMGFRDFRRINYWGRIPGVLGVYGAKVFYGN
                     QDANGSVETNALQIEMRLKEILRETGAAKVNIIAHSKGGIEARYLISTLKHADEVASL
                     TTISTPHNGSVTVDHLLKFPKFLVKFGCFLTDIFFRILGDKNPKTYSSVCSLTTKVAN
                     QFNLDNPDSDKVYYQSYAFVMKNIFSDGFFWLSWLLVRHYEGESDGLLSPRAVKWGNF
                     RGIYKSNGRRGISHCDEVDLRRRRFTKKTGDGISDITEIYLKIYEDLCNMGF"
     gene            complement(3646..6891)
                     /locus_tag="PLP02_03300"
     CDS             complement(3646..6891)
                     /locus_tag="PLP02_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPF42510.1"
                     /translation="MKKLTKIRLINWHYFANETIYIKNNTLITGQNATGKSTIVDAIA
                     FVITAGDQIFNLAANEKSKRDLRGYVKCKLGIDNQEYLRNGDVTGHIALEFFDEMDNT
                     YFTIGSVIDAFGEILAPKCIFYEKDGPIEESFFVDDEGRILTTLQFKKAKVADRIFTT
                     RREAKLAFRSQFGSVNENYFSLLPKALAFKPIPDVKDFIYHYMLEEKVLDVEPIRESI
                     QSYRDLEATLKIIKQKITSLQEIKDLYDDIQKNKEQKTFYEYFLKLIDIEATKDDIDK
                     KQKTQEKITVARDKNRKLIDEIDHQLELLDDRSKEIYSMLQSDSSFQAGEIYDKEIQS
                     VSRQIEEKEEINTYFKRKSDKLKALIKELKTEYKKPVYDELAKIDLFNLQKDGIDKTK
                     LALLDIDKRVKEIAEENQRESGSLERNRQDMVGEINEIYQTLKELDSHQLRYNPMVRR
                     LQKAIEEGVKERLGVDVSVHILAELLEVSDRSWHDTVEDYLGMQRFNLIVEPRYFDDA
                     LQIYNKVKEDINIYGIGLVNTKKIQNFTSCQAGSLASIIDSENVDAKHYINMICGNLI
                     MVQDVTDLEKYPQAITKSGMVYRSYTVRSLNRNTEKPFIGKRAYEEQLEKWRHLAKTK
                     KDEYQTLVDRINTLQEENNAIQALNLLENLKLIEASASLPELKTRKKELLEKKQNLPK
                     ENLNQIRMEYEGIKEEMRGFQNQRRHAYEENGKLSSDDLKCQDEILALETKINTLRKE
                     LESSAEGNLTLESDATKLYNQEIRNASDQKKVRDEYQKRIETELGNLSNLEDSLKSKQ
                     FKYVNTYNLSYQYGLEYMDLYLNEYNKLVKSELIKYEAKVREARESAEKLFKEDFLAK
                     LRSYIVAAQEEIRKINETLATIHFGDDTYEFIFPKSHEYGDYYDMVLSDESLKSGSEI
                     FNYEFEMKYQRQLEELFVNLAQEGLNDNGAVNKFTDYRTYMDYDIKIKNVSGDVILYS
                     KVFKEKSGGETQVPFYVATLASFVRLFQQAQRGNVHDVIGLIIFDEVFDKMDTSRIKS
                     MMEFIKRLPVQIILATPPQKMEVLSKFTDTTVVTLRDGRAARAYEVVQKDM"
     gene            complement(6869..7480)
                     /locus_tag="PLP02_03305"
     CDS             complement(6869..7480)
                     /locus_tag="PLP02_03305"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HPF42511.1"
                     /translation="MILENYENLNQGQKQMFSDTVNKLLSTGFLARDKRDNKEQYYFV
                     LSFKNYFDEYFGIINYELVLDREKGAIQLMSQENKSLLHLKREESLVLLILRILYHEH
                     LAETSINDNVIVRIDEIHQKYDALELKKKINKTDLLRIIRFYKRYNLIEPLGDIGQGN
                     TKVVIYPTVLLAIDTHEINDIFNLISRMNGQEGGSEDEETDEN"
     gene            complement(7477..8886)
                     /locus_tag="PLP02_03310"
     CDS             complement(7477..8886)
                     /locus_tag="PLP02_03310"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HPF42512.1"
                     /translation="MELFQIIPENFFNLLAGKNRILYMNALLEVFKAYERGSILGMDK
                     QLAQQAIIDYLDMNPQEEYVIDIDEQIDTEASNREKANQILRRLEECEWIDVDVDNDY
                     VEIVNFRDYAITIIEALKSLSLETIYGPDDDSHEFRGYIYTAYSLLTNEHGEYAMVVD
                     QVYKNTVAFVRELRKLDSRLKYYIRQIVEKSEIRDLVHLLVSYKVELVDHAYYRLKTS
                     DNVNKYKLEIVKRLEAMQQNPVVMEVIAREYLVKSNNNYDLAKVKANKRIDDMIDIYN
                     SLERIIDEIDEKNRVYVNTTIAKIKFLLNDDENVIGKLSAILKYTANMAKKHKTEKAL
                     KEISPLFTLSSHQQISKNSIFTPRGAYARADAQYLQENAETQDSKLEEEFYQEFETDF
                     SSDVIMKYLMEYFRKRPSIKASEIIREDMTDEAILRLLYILIYAGDELDYSIVPLQKE
                     IEHSRFRLQDFEIIRGYES"
     gene            complement(8903..9253)
                     /locus_tag="PLP02_03315"
     CDS             complement(8903..9253)
                     /locus_tag="PLP02_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF42513.1"
                     /translation="MTNSGEYMRGFTDYVVLSILSKFDSYGYEIGKIIENVSEQQIQV
                     TEAALYFALKRLLEDNKISSYREANKKGMNRRYYQITPEGKHSLNEFRKDWVQIESTI
                     SSLVEGEFEYTRED"
     gene            complement(9425..10213)
                     /gene="heR"
                     /locus_tag="PLP02_03320"
     CDS             complement(9425..10213)
                     /gene="heR"
                     /locus_tag="PLP02_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HPF42514.1"
                     /translation="METSIISKLRRFNLLMGALHLIQGVAMVFLATSVIQTIAEFKPM
                     ITQFYLTYDPIQMTLVVTSKDLFLLPFGILTAVFLLLSAFAHFLVSVPFNKVYNRDLE
                     KHINKFRWFEYALSSSVMIVLISTLFGIYDIASLVLIFVLNASMNMFGLLMEKINSQS
                     NETKKVNWSPFVYGSLAGIAPWVVIFLYMSGNPSLDMVPWFVWAITITYFVMFNLFPL
                     NMILQYKKVGKWSDYLYGEKTYIVLSLVAKTLLAWLVFFGAMQP"
     gene            complement(10326..10871)
                     /locus_tag="PLP02_03325"
     CDS             complement(10326..10871)
                     /locus_tag="PLP02_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42515.1"
                     /translation="MLFEQKTRMMKDGRECVLASPDPKDAKSMLDYLKTCAEETDNIL
                     RYPEECDESNEQEETFLRNINESQYNVMIVCKVDGDIAGNCTLTLNKRLKIKHRASVA
                     IALKRKYWNLGIGTMMFEEMEKIAREKNVLQLELGFVGGNDRARILYEKRGFVKTGKK
                     PDAFYLKDGSFRDEILMIKKL"
     gene            complement(10975..12003)
                     /locus_tag="PLP02_03330"
     CDS             complement(10975..12003)
                     /locus_tag="PLP02_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPF42516.1"
                     /translation="MCFMDIEIREEPEILQNARDKNIKTIIAVAQEFSKRNIRKIVLA
                     ARGSSDHAGVYFKYLSEIVLGIPVSLSAPSVVTMYDGKLDLSDTLTIGVSQSGEAKDV
                     LAVIERANNQHAVTVSLTNDPTSPLATKAAYHLDLSAGIEKSVAATKTFIAEMYLLAL
                     FVKELSGSPELAQEMEMLPELLENVLELDQKIAVLAAKLEDMEDCFVIGRGLSDAIAL
                     EIALKLQETSYVRAFGYATSDFRHGPFALVDKGSVAIILALEDETFPDSEDMVKRLNE
                     AKARIVVFTDNADFPVSDKIVLPKSGKYTKPFLFAAAGQLFSLYLAKAKGLNPDNPRN
                     LKKVTITR"
     gene            complement(12046..13173)
                     /gene="dnaJ"
                     /locus_tag="PLP02_03335"
     CDS             complement(12046..13173)
                     /gene="dnaJ"
                     /locus_tag="PLP02_03335"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPF42517.1"
                     /translation="MEKRDYYDVLGISKNATDEEIKKAYRLLAKKYHPDVSTEKDAEA
                     KFKEVQEAYDVLSDETKRAQYDQFGHQGMNGGFGGAGGFSGSGFEGFDFGDIFSAFFG
                     GQSRTNQGGRTRARKGSDIQKRMSISFEESIFGKKEKIRIPVYEECHVCHGTGAQSQS
                     DIHTCSKCHGTGTVIMETQTLFGRSQTRATCPVCHGTGKEIRNKCTNCNGEGVERVNK
                     EIEVNVPSGIETGQQIRLEGFGNKGTNGGPSGDLYIVFEVTPSNVYTREGDDLIINIP
                     ITFTQAALGAEIEVPTPYGNVLLKIPAGTQSDAKFRIRGKGAPNVRTKAYGDEHVHVT
                     VVTPSKLSAEQRKLFEQMAKIEDSPANKSAWSRFKSSFSRK"
     gene            complement(13241..15085)
                     /gene="dnaK"
                     /locus_tag="PLP02_03340"
     CDS             complement(13241..15085)
                     /gene="dnaK"
                     /locus_tag="PLP02_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010531040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPF42518.1"
                     /translation="MKIIGIDLGTTNSCVAVMEGKEAKVIPNLDGNRTTPSVVAFKDD
                     EIQVGEVAKRQVITNPNTVYSIKRKMGTTQKVEVNGKYYTPQEISAMILRNLKESAEA
                     YLGEKVTEAVITVPAYFNDAQRQATKDAGKIAGLDVKRIINEPTAAALAYGIDKKDKE
                     QTVLVYDLGGGTFDVSILELSDGTFEVISTSGDNHLGGDDFDQRIVEWLVDEFRKETG
                     VDLSKDKMALQRLRDSAEKAKKELSSITSAQISLPFISMTASGPVHLEKALTRAKFNE
                     LTHDLVERTIEPVKRALADAKLTAKDLDQVLLVGGSTRTPAVQDEVRKLLNKEPNRSI
                     NPDEVVAMGAAIQGGVLKGDVKDVLLLDVTPLSLGIETLGGVFTKLIDRNTTIPTSKS
                     QIFSTAADNQPTVDIHVLQGERSMARDNKTLGHFQLSDIPPAPRGIPQIEVKFDIDAN
                     GIVNVSAKNMATGKANSITIQSGTGLTDDEIDRMVKEAENNAESDRQKREEADLRNEA
                     DQMIFMTNKAIADLGDKVKDSEKKDAEEKIKKLEEALKGTDLEKIKKAKDDLEKTTQA
                     LSERVYKEQQNQQQGPASGGNDQGQGGSQDEGDSGSDDGVFDADYKEV"
     gene            complement(15103..15735)
                     /locus_tag="PLP02_03345"
     CDS             complement(15103..15735)
                     /locus_tag="PLP02_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPF42519.1"
                     /translation="MDENDKEKELPEVEEKQPEETTFGKDTKEPKDGKKKKNMEAELE
                     KAKDDLEEMSGKYLRTLAEMENLKKRLTDEMVRDRKYATMNVADKLIDHLEVFDQALS
                     MKTEDLNFKNFLLGFRMIKDSIFQVLSDEGVKQNNTVVGNNFDPNLEHAIDKRYEADK
                     PEGVILEVVKKGYMFKDRLLRPALVVINVRPEIKEDKESEEIETNDECVA"
     gene            complement(15742..16755)
                     /gene="hrcA"
                     /locus_tag="PLP02_03350"
     CDS             complement(15742..16755)
                     /gene="hrcA"
                     /locus_tag="PLP02_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPF42520.1"
                     /translation="MLTERQETILKLIVDEYVKTAEPVGSKALSFKMEISSATIRNEM
                     SALEDAGYLEKTHTSSGRIPSEQGYRYYIEKIIRNIDEESDGYFEFEKLFENKEIERE
                     ELIKEAIDLLSQVTNCTAISLGPEAYYSKVKKVQMIPLKETQALILVVTNFGHVESKQ
                     ITISENMEVAEMVKVIDLLNEMLVNTPISQVSEKLHYEIQHSQIKELLKYRESIVDSF
                     IEAFSKFAQTRYYLTGQSNMLYQPEFNDIQKLRKFIETVENNDIFKLVETDSDGVSVK
                     IGTENQVTSMKDCTVVSSTYDTIDGEKGTISVVGPTRMDYRKVIPLLKYIAKNIAKLY
                     EEE"
     gene            complement(16930..17553)
                     /locus_tag="PLP02_03355"
     CDS             complement(16930..17553)
                     /locus_tag="PLP02_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF42521.1"
                     /translation="MTHQRIYDNIALQNTYVLQKKSAAIVIDPGFNGNEAVNCLKESG
                     AIEVAVFLTHGHFDHIRDLPVILEAFPKSRVYIQENDLQFLYDERLSYARAFHSHFSL
                     PKEQTVETFGMQGELVFEDIVIRYLNTPGHTAGSASLYIDERLYSGDTLFSDGIGRTD
                     LYTGSMASMNKSLKLLITSFSRKTLVCPGHGESKRLGEIIENNPYLP"
     gene            complement(17555..18481)
                     /locus_tag="PLP02_03360"
     CDS             complement(17555..18481)
                     /locus_tag="PLP02_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPF42522.1"
                     /translation="MPEYLLGIDIGGTSVKIGLFNLEGFLLDKRSIITDRSQNGSHIL
                     RDIAVFIKKNWLLSSIAGIGLGVPGPVKNNIVETCVNLGWGITDPKTELNKYFNYEDI
                     AIKVTNDASAAALGEYAAGAAKGYSNVVLLTLGTGIGGGVIIDGRLHEGVDGISGEFG
                     HIVVDKKHAFLCNCGKRGCLETVASATGIARLAKYNLDNTDLSSKLRNMDSFSAKTVF
                     DLAKEKDEVAVLTIDEAADNLAAMLGSITLTIDPDLFVLAGGVSLAGDYLLQSVKRHY
                     PDYIRPFDVKAGIVLATLGSDAGIYGAMQLVK"
     gene            complement(18549..18701)
                     /gene="rpmG"
                     /locus_tag="PLP02_03365"
     CDS             complement(18549..18701)
                     /gene="rpmG"
                     /locus_tag="PLP02_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPF42523.1"
                     /translation="MVRVQFTMRCTECKEENYTMTKNKKNHPDRMEVMKYCPRCRKET
                     LHREKK"
     gene            complement(18789..19571)
                     /locus_tag="PLP02_03370"
     CDS             complement(18789..19571)
                     /locus_tag="PLP02_03370"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPF42524.1"
                     /translation="MWLIIGGIILYFAYFSIIYLIAKRKGNYGYVDIAWGFGFVVYVF
                     FATAYRFQTSDHILISQAVCFIVVILWGLRLFVHVMLRNYGKPEDFRYQNMRKKWEGK
                     HPGLKAYVNIFLLQALLAYIVSLPITMVFISDSEKSVVTAYIGLAISLLGLTLETISD
                     AQLGIFRKNPESRGHVMTEGLWKYSRHPNYFGETVVWWGLFIGVFLSDFGAYSLLSPL
                     IITYLLLYVSGVPLLEKHYEGNPEYEEYKHRTSVFFPLPPKK"
     gene            complement(19573..21324)
                     /locus_tag="PLP02_03375"
     CDS             complement(19573..21324)
                     /locus_tag="PLP02_03375"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPF42525.1"
                     /translation="MELITLKGNDLYQAFLAGARRLISRKKYLNSINVFPVPDGDTGT
                     NMSFLMQSIIDKAKSSEDLSETAASIATAAVDGSRGNSGIIFAEFFVGLYEKLKNLRQ
                     ARLEDFRQAISYAIFRSYQAIMNPVEGTILTVLRKAFDIKERLTDFKTYFRTSLEQAK
                     TALKETPDELESLKKNGVVDAGAEGFTTFWEGVAIYMETGERESADLKEADVSFDIIY
                     DPDYDMGKRYCTEAVILDGKTELSSVKSALNDIGDSLIVSGKPEKMHVHIHTDNPEQV
                     FLRLRTYGRLNWQKIDDMKLQQQAMAKDRPETVVVVDSIADIPQDLMDTYQIHMLPMN
                     LIVDDISYLDKQSVKIDTFYEMMDKYSTFSSSQPDSGTIERLLGFLSEHYENILVVTV
                     AGALSGTYDALCKYAGTNSKVKVFDSLQNSGAEGLLALEAARMASDGIKIEEIMAKLE
                     EKRSKTRIYVSVKTLKYMVKGGRVGKVTGMIAKILNLKPVVSLDGTGHGIIREKAFSI
                     RQNEKKILKLLQKAPIVKYSIIHADALNRAEKMAEKITKLCGKAPEFITEISPIVAAN
                     AGTGAVGIAVEFSREVK"
     gene            complement(21332..21718)
                     /locus_tag="PLP02_03380"
     CDS             complement(21332..21718)
                     /locus_tag="PLP02_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HPF42526.1"
                     /translation="MTYLKLYAIALGFFLVIDMLWLGVVAKKVYDRYLGGYIGKVKWI
                     PAIIFYLVFVAGLVFFVIYPAVGRESLNYAIYASMFFGFVAYSTYDLTNMATLKDWPW
                     QIVLIDIAWGMILGGIVSTVTYLIAR"
     gene            complement(21846..23000)
                     /locus_tag="PLP02_03385"
     CDS             complement(21846..23000)
                     /locus_tag="PLP02_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HPF42527.1"
                     /translation="MKVACLNKISPFGLTRFTNGYELTEEVNDADLILVRSFNMLEME
                     LPKKLLAVARAGAGVNNIPLADLASKGIVVFNTPGANANGVKELVIAGMLLAARDIVG
                     GVNWVRDNKEDANLSKSVEKAKSAFGGTEIIGKKLGVIGLGAVGGKVAEAAICLGMSV
                     LGYDPYLSPENADKLPNEVELTTELDRIYATCDYITLHLPLLDSTRHMIAKDVFAIMK
                     PGVVLLNFSRDQLVNDDDLAISIESGIVRSYVTDFPNPKTANMNHVIAIPHLGASTEE
                     SEDNCAMMATDELMRYVEHGSIKNSVNFPALDPGRKQNKYRIQILYKDSEEFLGSLTR
                     ALEGIVSLGNLINRAKNGYGVAVVDFDAETNEAAIEISKKMKGVLRVRTL"
     gene            complement(22997..24082)
                     /gene="serC"
                     /locus_tag="PLP02_03390"
     CDS             complement(22997..24082)
                     /gene="serC"
                     /locus_tag="PLP02_03390"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPF42528.1"
                     /translation="MKRVFNFSAGPAVLPEPVLRQAAAEMLDYNDTGMSVMEMSHRSK
                     AFDQIIQDAEKDLRTLLSIPDNYHVLFLQGGGHTQFAMVPMNLMKNGVADYIVTGEWA
                     KKAHNEAKKYGDARKIATSEDKMFSYIPDLDNLPISENADYVYICTNNTIYGTKFAKE
                     PDTKGKVLISDMSSCILSEPIDVTKYGLIFAGAQKNIGPAGTTIVIIRDDLIRTDLPE
                     FVPTMLKYHIHAEKNSLYNTPPTYGIYICGLVFKWLLNLGGLEKMQEINIAKASMLYD
                     FLDHSKMFHGSVEKKDRSLMNVTFGTGNPEMDAKFVAEAKAAGYDNLKGHRSVGAMRA
                     SIYNAMPIEGVESLIDFMKKFEKENAK"
     gene            complement(24066..25283)
                     /locus_tag="PLP02_03395"
     CDS             complement(24066..25283)
                     /locus_tag="PLP02_03395"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPF42529.1"
                     /translation="MEVVSSKEILLPKQSIDHTLWSVIACDQFTSEISYWERLRQQVE
                     DVPSTYHLILPEAYLHLGFETRIQSANSNMNLYLRSNIFDTVSNPIIIDRKTPYHEHR
                     YGLLLTIDLEEYEYMPGKKGRIVASEKTVLDRIPPRVKIRENAPIELPHILVLFDDVD
                     GLIAKTASDTRNMKKLYDFDLNMGGGHITGHLLEKSAELLAKINEQIEKTPLIVGDGN
                     HSLASAKVCWENLKKNLPEEELVGHPARYALVELESIYDKGLAFEPIHRVIYRPGDDF
                     LEDLSRASYGQNPMKILTKKGEDYILSPESPFETIVWIQNFLDDYLAFHPDTRIDYVH
                     GFKNLEGIIAENPGSIGLVLPPLGRDKLFPYINENGVLPRKSFSLGEAIEKRYYLECR
                     LITKGRIDNEKSI"
     gene            complement(25287..>26264)
                     /locus_tag="PLP02_03400"
     CDS             complement(25287..>26264)
                     /locus_tag="PLP02_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPF42530.1"
                     /translation="MGGGKGGADFDPKGKSDDEIMRFCQNFMLELYRHIGPDTDVPAG
                     DIGVGFREVGYMYGMYKKIQNESTGVLTGKGLTYGGSLVRKEATGYGLCYFVQEMLRV
                     YRNDEMKDKRIIISGSGKVGLYAALKARELGAKVVAMSDVNGCVYDPNGIDIDIVKML
                     TKDMPEFSTKYLEYRSNAVYNSNPTEIWKLPCDIALPCATQNELPLEGAKALVENGCF
                     LVAEGANMPTTIEATKYLQHADILFAPGKAANAGGVAVSGLEMSQNAMHYPWTYEEVD
                     EKLEVIMADIFNKAYTEARKYTDHRNLVIGANIAGFMRVYQAMLDQGVY"
BASE COUNT         6208 a         6114 c         6012 g         7930 t
ORIGIN      
        1 aacaaaaaca agcgctacaa cggcaacatc cttaacgaaa ccattgtttc cggaaacctg
       61 atgagagacg agaccaacac gtacaaatgc ctgaacggaa aaggcaagcg gatggtcaga
      121 aagtaccttg agtactatgt caagaatctg ccggaactgg tattgttgag aagttttata
      181 tcggccgaca agaaaaaagc atcttttgtt cttttgtatc tgaatgagat gaacaaagtc
      241 attttccgct gggatcgttc caaaaagatt cgcaatctgg tcaaaggcat caacaagtac
      301 cagatgcttc tgccgacgat cggatttttg ttcgaagtcc cgatgcagat caacgaaggc
      361 gtgccatacc ggatgacaaa tcatgacaac ggcaccgtga tcagttacga tgtcgtcaaa
      421 gatgccaaac atatcaagag aaaacaaaag gaaaaaccgc tttcgaagaa acagatcaag
      481 gctcaggcca agatcgaagc tttgaaagaa aaacggctcg ccaaacagaa aaaacgttga
      541 atttgtttct ttaaaagatc ctcggaaaac cggggatctt ttttatttat tcacaagcga
      601 gccgacgctt agagggtatg cttccctatc caggatgaca tccaccttcc ggccgattaa
      661 atcgtccgaa cctttgaaag ccacgtggat gtagccggcg gaatgtcccg tcaaataatc
      721 tcccttcctt ctttcgacga tgacttcgag tttttttccg atcattgctt ttatcgcgtc
      781 ttttgccatt ttctcgccta aagctatcag acgaccgact ctttcttttt ttatgtttcc
      841 gggaatttcg tcggtcattt tcgccgctac ggttcccggc cgtttggaat atgggaatac
      901 atgcaattcg ttaaatccga tcatttccgc aaatgtcagc gtatctacaa agtcggcttc
      961 cgtttcttta gggaagccga cgatgatgtc ggtagtcact gacagatctg gcaaagcctt
     1021 acggatttta tttatcctgt ctttgtaata agcggtgtca tatttgcggt tcatcaattt
     1081 caggattttg tcggatccgg actggagagg aatgtgaagg tgatgcacaa aacgctttga
     1141 accggaaaga atttcgatca cttcatcagt cagttcatgg atttcgatcg acgatatgcg
     1201 aatccttgga aggttattat caccagccag tttccaaaga agcgtcgcga aggtcacatc
     1261 ttcaatatcc ttcccgtatc cgccggtatg aattcccgtc agaatgattt cctgatggct
     1321 ttttgctagt atttcggctt cttccatgac ttcgtcaagc ggtttggaac gaaccggtcc
     1381 gcgagcgtaa ggaatgatgc aataggaaca gtaattatcg catccgtcct cgattttcaa
     1441 aaatgccctt tgatgatcag aatatccgga aaccgccaga ttcccgaatt tacgacgatt
     1501 ttcaacacgg tttataccat tgattttttt gcctttttca tggtattcat ccagatatcc
     1561 aagcagcttc tcccgatcat tcgttccgac aatcgcccgc acgttttttt ccgcaagaac
     1621 tttttcagct tctaattggg acaaacatcc catgactagg atgtcggcat ccggattgtg
     1681 tttcaaaacc cggtgaatca tctgtctgct tttgccggca gcggtgtttg tcacaaggca
     1741 ggtgttgaca atatagacat cggccgcttt tgtgtcacta accctttgat agcctttttg
     1801 tctgaaaatc tcccacaagg cttcggtttc gtaggaattg actttgcatc ccaaagtggt
     1861 gaaggcaact tttttcatat catctgcctc cttcatacaa gaaggcggaa agaaccatga
     1921 tcgatgcggt ttcgctccgc aggattctat ttcccaaagt tacgaaaaaa gctttttgaa
     1981 ggctaagaaa ctccgcttct tccttcgata tgccaccctc cgggccgaca atgacgagga
     2041 ttttttcttc gggacggatt tgtccgatga tttctttcaa tgatccgtcc gtgtcgtttt
     2101 tttcataaca gacgatcacc cggtcatatg ccggataatc gatatctgtg agaattgtaa
     2161 aaggaagcag ttccgggata tcttttcgct ttgactgttc ggaagcttct ttcagaatca
     2221 gttgaaaccg ttcatgcttt cttgattctt tcgtttcgtc gattttgact accgagcgcg
     2281 aaaaaaccgt cgggattaca gcgctgacgc cgagttccgc cgctttttcc agaaacagtt
     2341 cgaaccggtc ctttttgatc aaggcctggg cgatcgtgac attgtagtaa ggttcaatta
     2401 tattcaactc ttccgtaagc gtcacttcgc agatatccct ggtgatcaat ccgatcacgc
     2461 ccagataact ttttcctccc gaaattccga ttataacctg ttgcccggta tggtaacgca
     2521 aaacgtcccg gatatgatga agatcggatc cggaaagata acaggtatgg tcgtggactt
     2581 tatcgggaag aacgaaatac ttttgcatat ttttcaccgc ttcaatgata acataaaatc
     2641 atttttttga taacaaaaag ccgcaccaga ctgcgacttc tgtcatttca gaatcccata
     2701 ttgcatagat cttcatatat tttcagataa atttcggtga tgtcggaaat accgtcaccg
     2761 gtctttttgg taaaccggcg tcttctcaaa tcgacttcgt cacaatggga gattcctctt
     2821 cttccgttgc ttttatagat gcctcggaaa ttcccccact tgaccgcacg aggcgacaac
     2881 aagccgtcgc tttctccttc atagtggcgg acaagcaacc aagataacca gaaaaaaccg
     2941 tccgaaaata tgtttttcat gacaaaggcg taactttgat agtaaacctt gtcggagtcc
     3001 ggattatcca ggttgaactg gttggccact tttgtggtca aagaacagac ggaactgtac
     3061 gttttcgggt ttttgtcgcc gaggatacga aagaaaatat cggtgagaaa acaaccgaac
     3121 ttgacaagaa atttcgggaa tttcaataga tggtcgacgg tcacagaccc attgtggggc
     3181 gtcgatattg tcgtcaggga cgcgacctcg tccgcatgtt tcaacgtcga tatgagataa
     3241 cgggcttcga tcccgccttt cgaatgggcg atgatgttga ctttagcggc acccgtttcc
     3301 cttaaaattt ctttaagtct catttcaatt tgaagggcgt tggtctcgac cgaaccgttg
     3361 gcgtcctggt ttccatagaa aactttggcc ccgtatactc ccaaaacgcc gggaatcctg
     3421 ccccagtaat tgattctacg gaaatcccga aatcccattc cgtgtaacaa aagtatcgga
     3481 tagatcgttt cttcggccat ataaggtatt ccttcctcta aggtcatgtt ggtttgaacc
     3541 gccatatcgt tattttatca tatcgcgtca taccatgaaa ggcaaaaccg tcttttgttt
     3601 ctgttttata aaaaaacggc tcccgcaaag gagccatttc atttatcaca tatctttctg
     3661 gacgacttcg taagctctgg cagcccgtcc gtcccttagg gtcaccacag tcgtgtcggt
     3721 gaacttgctc aagacttcca tcttctgcgg cggggttgcc aggatgatct ggaccggcaa
     3781 acgcttgatg aattccatca tcgacttgat tctggatgta tccatcttgt cgaagacttc
     3841 gtcgaagatg atgagtccga tgacatcgtg gacgtttccg cgttgagcct gttggaagag
     3901 tctgacaaac gaggcaaggg tcgcgacata gaacggaacc tgggtctcgc cgccggattt
     3961 ttccttgaaa accttggaat aaaggatgac gtcgccggag acgttcttga tcttgatgtc
     4021 gtaatccatg taggtccggt agtcggtgaa cttgttcaca gcgccgttgt cattcaggcc
     4081 ttcctgggcc aggttgacga atagttcctc cagttggcgc tgatatttca tttcaaactc
     4141 gtaattgaag atttcggaac cgctcttcag ggactcgtct gacaacacca tgtcgtaata
     4201 gtcgccatat tcatgagatt tcgggaagat gaactcgtag gtatcgtcac cgaaatggat
     4261 ggttgcgaga gtttcgttga tcttgcggat ctcctcctgc gcggcgacga tgtaacttct
     4321 gagtttcgcc aggaagtcct ctttgaagag tttctcggca gattctctcg cttctctgac
     4381 tttggcttcg tatttgatca gctcggactt gaccaatttg ttgtattcgt ttaagtaaag
     4441 gtccatgtat tcgaggccgt attgatagga tagattgtag gtgttgacgt atttgaactg
     4501 tttagatttg agcgaatcct cgagattgga cagatttccg agttcggttt cgatccgctt
     4561 ctggtattca tcgcggactt tcttctgatc ggaagcgttc ctgatctcct ggttgtaaag
     4621 cttggtggca tcgctttcta aggtaaggtt gccttcggcc gatgactcca attcttttct
     4681 tagggtgttg attttggttt ccaaggcgag gatctcgtcc tggcacttga gatcatcgga
     4741 agacagtttt ccgttttctt cataagcatg ccggcgttga ttctggaagc cgcgcatttc
     4801 ttccttgatg ccctcgtatt ccatacggat ctgattcagg ttttccttcg gaagattctg
     4861 cttcttctca agcaactctt tttttctagt cttcagttcc ggtaaggatg ccgatgcctc
     4921 gatcagttta agattttcca gaagattcaa ggcctggatg gcattgtttt cttcctgcag
     4981 ggtgttgatc cgatcgacaa gcgtctgata ctcgtccttt ttcgttttgg caaggtggcg
     5041 ccatttttcc aactgctctt cataggcgcg tttgccgata aacggctttt cggtgtttct
     5101 attcagactt ctgacggtat aggaccggta taccatgccg gatttcgtaa tggcctgggg
     5161 atatttctcc agatcggtga catcctggac catgatcaga tttccgcaaa tcatgttgat
     5221 gtaatgtttg gcatcgacat tctcggaatc gatgatcgag gccagcgatc cggcttgaca
     5281 ggaagtgaaa ttctggattt tcttcgtgtt gacgagaccg atgccgtaaa tattgatgtc
     5341 ttctttgact ttgttataga tttggagggc atcgtcgaaa taacggggtt ccacaatcaa
     5401 attgaatctt tgcattccga gataatcttc gacagtgtcg tgccacgaac ggtcgctgac
     5461 ttccagtaat tcagccagaa tatggacgct tacatccact cccaaacgtt ccttgacgcc
     5521 ttcttcgatc gctttttgca atcttcttac catcgggttg taacgaagtt ggtgggaatc
     5581 gagttctttc agggtctggt agatctcgtt aatctcacct accatgtcct gacgatttct
     5641 ttcgaggctg ccggattctc tctggttttc ctcggcgatt tcctttacgc gtttatcgat
     5701 gtccaaaagc gccagtttgg ttttgtcaat cccgtctttt tgcaggttga agaggtcgat
     5761 tttcgcgagt tcgtcgtaga ccggtttttt gtattcggtc tttaactcct taatcagggc
     5821 tttcagcttg tcggatttgc gtttgaaata ggtgttgatc tcttcctttt cttctatttg
     5881 acgagacaca gactggattt ccttgtcgta gatctcgcca gcctgaaagc tcgaatcgga
     5941 ttgcagcatt gaatagattt ccttgctgcg gtcgtccagg agttccagct ggtggtcgat
     6001 ctcatcaatc agtttgcggt ttttatccct ggcaacggtg attttttcct gggttttctg
     6061 ttttttgtcg atatcgtctt tcgtcgcttc gatgtcgatc agtttcagaa aatattcata
     6121 gaaggttttt tgttctttgt tcttttggat atcgtcgtaa agatccttga tttcctgtag
     6181 cgacgtgatc ttctgtttga ttattttaag cgtggcttcc agatcgcgat atgattgaat
     6241 ggactcccgg atcggttcga catccaaaac tttttcttcc aacatataat gatagatgaa
     6301 atccttcacg tcgggaatcg gcttgaaagc caatgccttc ggcaacaatg agaaataatt
     6361 ttcattcacc gaaccgaact ggcttctgaa agcaagtttt gcttctcttc tggttgtgaa
     6421 gattctgtcg gctactttgg ctttcttgaa ttgaagcgtc gtcagaatgc ggccttcgtc
     6481 gtccacgaaa aagctttcct cgatgggacc atccttttca tagaagatgc acttgggagc
     6541 gagaatctcg ccgaatgcgt cgatgacgga accgatcgtg aaatatgtgt tgtccatttc
     6601 atcgaagaat tcgagagcga tatgtccggt gacatcgccg tttctcagat attcctggtt
     6661 gtcgataccg agtttgcact tgacatagcc tctgagatcg cgtttgcttt tttcgttggc
     6721 cgccaagttg aagatctggt cgccggcggt gatcacgaaa gcgatggcat cgacaatcgt
     6781 cgattttccg gtcgcgtttt gacccgtgat cagggtattg tttttgatat agatggtctc
     6841 attggcgaaa taatgccagt tgatcagtct aattttcgtc agtttcttca tcttcactgc
     6901 ctccttcctg gccgttcatc cggcttatca ggttgaaaat atcgttgatt tcatgggtat
     6961 cgatcgccaa caggaccgtg ggatagatca ccactttggt attgccctga ccgatatcgc
     7021 caagcggttc gatcaagtta taccttttgt agaaacggat gattctcagt aagtcggtct
     7081 tgttgatctt ctttttcaac tcaagggcgt cgtatttctg atggatctcg tctattctca
     7141 cgatgacatt gtcgttgatc gaggtctccg ccagatgctc gtggtagaga atcctcagaa
     7201 tcagaagcac gagcgattcc tcccttttga gatgcaggag gcttttgttc tcctgactca
     7261 tgagctggat ggctcctttt tccctgtcaa gcacaagctc gtagttgatg atgccgaagt
     7321 attcgtcgaa gtaattcttg aagctgagaa caaagtaata ttgttccttg ttgtcgcgct
     7381 tgtcccgggc cagaaatccg gtcgatagca gtttgttcac tgtgtctgag aacatctgct
     7441 tttgtccctg gttaagattt tcgtagtttt ccagtatcat gattcatacc ccctgattat
     7501 ttcaaagtct tggagccgga aacgactgtg ttcgatttcc ttctggagcg gcacgatcga
     7561 gtaatcgagt tcatcgcccg catagattaa gatatagaga agtctcagaa tcgcctcatc
     7621 tgtcatatcc tcgcggataa tctcggaggc tttgatggaa ggcctctttc ggaagtactc
     7681 catcagatat ttcattatca cgtcgcttga gaagtcggtt tcgaattcct gatagaattc
     7741 ctcctccagc ttgctgtcct gggtttcggc gttttcttgg agatactggg cgtcggccct
     7801 tgcatatgcc cctcttggcg taaagatcga attcttggaa atctgctgat gactcgacag
     7861 ggtgaagagc ggtgaaatct ccttcaaagc cttttctgtt ttgtgcttct tcgccatgtt
     7921 ggcagtgtat tttagaatag ccgagagttt accgatgaca ttctcgtcgt cgtttagcaa
     7981 aaactttatc ttggcgattg tcgtatttac gtataccctg tttttctcgt caatctcgtc
     8041 gatgattctt tctagcgagt tatatatgtc gatcatgtcg tcgattcgtt tgttcgcctt
     8101 gactttcgcc agatcgtaat tgttgttcga cttgactaga tattccctgg cgatgacttc
     8161 catcacgacc ggattctgtt gcattgcttc aagccgtttg acgatctcca atttatactt
     8221 gttgacattg tcggatgtct tgagccggta ataggcgtga tcaaccagtt ccactttgta
     8281 actcactagc aagtgcacta aatcgcggat ttccgatttc tcgacgattt gccggatgta
     8341 atacttgagg cgcgagtcga gttttctcag ttccctaacg aaggccacgg tgtttttgta
     8401 gacttgatct accaccattg cgtattcgcc gtgctcgttc gtaagcaggc tgtaagccgt
     8461 atagatatac ccgcggaatt catggctgtc gtcatcgggg ccgtaaatgg tttcgagact
     8521 caggcttttc aaagcctcaa tgatcgtaat cgcgtaatcg cggaaattga cgatctcgac
     8581 atagtcgttg tcgacatcga catcgatcca ttcgcactcc tcgagcctcc tcagtatctg
     8641 attggccttc tcgcggttag atgcttccgt atcgatttgt tcgtcgatgt ctatgacata
     8701 ctcctcttgc ggattcatgt ccagataatc gatgatcgcc tgctgggcca gttgcttgtc
     8761 catcccaagg atcgagcccc tctcataggc tttgaacacc tcgagcaggg cattcatgta
     8821 caagatgcgg ttttttcctg ccaggagatt gaagaaattc tccgggataa tttgaaatag
     8881 ttccatcttc tcacttcctc ttttaatctt ccctggtata ttcgaactcg ccctcgacca
     8941 gactggatat cgtgctttcg atctggaccc agtctttacg aaactcgttc agggagtgtt
     9001 tcccctcggg ggtgatttga taataacgac ggttcatgcc ctttttgttg gcttcgcgat
     9061 aggatgatat cttgttgtct tccaaaagtc gtttcagggc gaagtacagg gcggcttcgg
     9121 tgacctgaat ctgttgttca ctgacgtttt cgatgatttt tccgatttcg taaccgtagc
     9181 tgtcgaattt ggacaaaata gacaaaacga cataatcagt gaatccccgc atatattcgc
     9241 cggaattcgt cattatccca cctcctttat atataagttg tttatgtata aattgttttt
     9301 gcatagattg tttatgcata acctgtttat attatacctg cttgagtatc gcatgtaaag
     9361 aaaaaaatga attatatata aaaaaaccga tcctcgaaag gatcggctta gcttgtatca
     9421 tatattacgg ttgcatggct ccgaagaaaa ccagccatgc gagcagggtt ttcgccacta
     9481 ggctcaaaac gatgtaggtc ttttctccgt atagataatc cgaccatttt cccacttttt
     9541 tatattgtag aatcatattg agcgggaaca ggttgaacat gacgaagtag gttatcgtga
     9601 tcgcccacac gaaccatggc accatatcaa gcgagggatt accggacatg taaagaaaga
     9661 tgacgaccca aggcgcgatg ccggctaacg agccataaac gaaaggcgac cagtttactt
     9721 ttttggtttc attgctttgg gaattgattt tttccatcaa aagaccgaac atgttcatcg
     9781 aagcgttcaa gacaaagatc agtacaagcg aagcgatgtc ataaataccg aaaagtgtcg
     9841 atatgagcac tatcatcact gacgaggaaa gcgcgtattc gaaccaacgg aatttgttga
     9901 tatgtttttc taggtcgcga ttataaactt tgttgaaagg aacgctgacg agaaaatggg
     9961 caaaagccga caaaagcagg aaaactgccg ttaagattcc aaacggcaac aggaataaat
    10021 ccttgctcgt aacgacaagc gtcatctgga tcggatcgta tgtcagataa aactgcgtga
    10081 tcatcggttt gaattccgcg atcgtctgaa tgacggatgt tgcgagaaac accatggcca
    10141 ctccctggat cagatgcaag gcgcccatca gaagattgaa acggcgaagt ttggaaatga
    10201 ttgacgtttc cattgaatta ttctcctttt attatatata gatatgtaag caatatatac
    10261 tttgatattc aaattatatc ataagaaaaa gcgccaggca aacaaattgc tcggcgcgca
    10321 tgaagtcata atttcttaat cattaagatt tcatcccgga aggatccgtc ctttaagtaa
    10381 aaagcgtccg gctttttacc tgttttgacg aatcctcttt tttcatagag aatcctggct
    10441 cggtcgtttc ctccgacaaa tcctagttcc agttgtaaaa catttttttc tctggctatt
    10501 ttttccattt cttcgaacat catggtgcca atgcccagat tccagtattt tcgcttaaga
    10561 gcgatcgcca cgcttgcccg gtgtttaatc ttcaatcgtt tatttaacgt gagcgtacag
    10621 ttgccggcga tatcgccgtc aactttgcag acgatcatca cgttgtactg tgattcgttg
    10681 atgtttctta gaaacgtttc ttcctgttcg ttgctttcgt cgcattcttc cggatatcgg
    10741 agaatattgt cggtttcttc ggcacatgtt ttgagataat cgagcatgga tttggcatct
    10801 ttcggatccg gacttgccag aacacattcc cggccatctt tcatcattct ggttttttgt
    10861 tcgaatagca tggctgtcat ctccttccga tgtcttttct ttattgtatc acacgctttt
    10921 tttcttttgt atacggatac aaaaaaacag tcctttcaca gactgttttc aggttcatct
    10981 tgttattgtc acctttttta aatttcgggg attatcggga ttaaggcctt tcgccttggc
    11041 gagatacagc gaaaacaatt gtccggctgc ggcaaacaga aacggcttgg tatatttgcc
    11101 ggattttggc aaaactattt tatcggaaac cggaaaatcg gcattgtccg taaaaacgac
    11161 aattctcgct ttggcttcgt tcaagcgttt taccatatcc tcgctatcgg ggaatgtttc
    11221 gtcttctagt gccaggatta tggcaaccga tcctttgtcg accagggcga aaggcccgtg
    11281 tcggaagtcg cttgtcgcat aaccgaacgc gcggacataa gacgtttcct gaagtttcag
    11341 ggctatttcc agggcgatag cgtcagaaag tcctctaccg atgacaaaac aatcttccat
    11401 gtcttcaagt ttggctgcga gcacggcgat tttttggtcg agttctaaga cgttttccaa
    11461 taattcggga agcatttcca tttcctgggc cagttcaggg gaacctgaca actccttgac
    11521 aaaaagggca agcagataca tttccgcaat gaaagtttta gtggcggcca cactcttttc
    11581 tatgcctgcg gacaaatcga ggtggtacgc ggcctttgtc gccagcggtg atgtcggatc
    11641 gttggtcagg gaaacggtta cggcatgttg gttgttggcg cgttcgatga cggccaggac
    11701 atccttggct tctccgcttt gggaaacgcc gatcgtcaac gtatcggaaa gatccagttt
    11761 gccgtcatac attgtcacaa cgctcggggc ggaaagcgaa accggaattc ccaagactat
    11821 ctccgacaga tatttgaaat atacaccagc atgatcggaa gatccccttg cggcaaggac
    11881 aatttttctg atgtttcttt tagaaaattc ttgggcgacc gctatgatcg ttttaatatt
    11941 tttgtcccgg gcgttttgca gaatttctgg ttcctcccgg atttcaatgt ccatgaaaca
    12001 catgagatat cacctcttta tggcaaaaat agcccgaagg ctatttcatt tacggctgaa
    12061 actcgatttg aaccggctcc aagcgctttt gtttgccgga ctgtcctcga ttttcgccat
    12121 ttgttcgaat agttttcttt gttccgccga aagtttggaa ggcgtgacga ctgtcacgtg
    12181 gacgtgttcg tcgccgtaag ccttggtacg tacgttcgga gcgccttttc cgcggatgcg
    12241 gaatttggca tccgactggg ttccggccgg tattttcaga agcacgttgc cataaggcgt
    12301 cggtacttcg atttccgctc ctagggcggc ttgggtgaag gtgatcggaa tattgatgat
    12361 gagatcgtcg ccttcgcggg tatagacatt agacggagtg acctcgaata cgatgtaaag
    12421 atcgcccgaa ggtccgccgt tcgttccttt gtttccaaat ccctcgagac ggatctgttg
    12481 tccggtttcg attccggaag gaacgttaac ctcgatttcc ttattcaccc gttcgacgcc
    12541 ttcgccgttg cagttagtgc atttgttgcg gatttcttta ccagttccgt gacagaccgg
    12601 gcatgtagcc ctggtctgac tgcgtccgaa cagggtttgc gtctccatta tcaccgttcc
    12661 ggttccgtga cacttggaac atgtatggat gtctgactgg ctttgggcac ccgtgccatg
    12721 gcagacatgg cattcttcat atacgggaat ccggattttt tccttttttc caaaaatcga
    12781 ttcctcaaag gatatggaca tccgtttctg gatatccgaa ccttttctgg ctctcgttct
    12841 cccgccttgg ttcgtacggg actgaccgcc gaaaaaggca gagaaaatat ctccgaaatc
    12901 gaagccttcg aaaccggaac cggaaaaacc gccggcgccg ccaaatccgc cgttcatgcc
    12961 ttggtgtccg aattgatcgt attgggctct tttcgtctca tccgacaaaa catcgtaggc
    13021 ttcctggact tccttgaatt ttgcttcggc gtctttctcg gtggagacgt cgggatgata
    13081 tttttttgcc agtaaacggt aagccttctt gatttcttca tccgtggcgt tcttgcttat
    13141 gccaagtaca tcgtagtaat cgcgcttttc catgtcctca cctcattgac gcgacaaaaa
    13201 gcagttgcct gctttttgtt tgattctgtt aatgtttttt tcagacttcc ttgtagtcgg
    13261 catcgaagac accgtcatcg gagccggagt cgccttcatc ctggctgccg ccctgacctt
    13321 ggtcattgcc gccggatgct ggtccttgtt gctggttttg ctgctccttg tatactcttt
    13381 cggaaagtgc ctgggtcgtc ttttccaagt cgtccttggc ttttttgatt ttttccagat
    13441 cggtgccttt taaagcttct tccagctttt tgattttttc ttcggcgtct ttcttttcgc
    13501 tgtccttgac cttgtcgcca agatcggcga tcgccttgtt tgtcatgaaa atcatctggt
    13561 cggcttcgtt tctcaagtcc gcttcctcac gtttctggcg gtcggattcg gcattgtttt
    13621 ccgcttcttt gaccatccgg tcgatttcgt catctgtgag tccggtgccg ctttggattg
    13681 tgatcgaatt ggctttcccg gtcgccatgt ttttagcaga tacgttcacg atgccgttgg
    13741 cgtcgatatc gaatttgact tcgatctgcg ggataccgcg cggtgccgga ggaatgtcgc
    13801 tcagttggaa atgtcctaaa gtcttgttgt ctctggccat cgaacgttcg ccctgaagca
    13861 catggatgtc gacggtcggc tgattatcgg ccgcggtcga gaatatctgg gatttcgatg
    13921 tcgggattgt cgtgttgcgg tcgatcaatt tcgtgaagac gccgcccaag gtttcgatac
    13981 cgagggaaag cggagtgacg tcgagcaaca atacatcctt gacatcgcct ttcaggactc
    14041 cgccttggat ggcggctccc atggcaacca cttcgtccgg gttgattgaa cggttgggtt
    14101 ctttattcaa aagttttctg acttcgtcct gaacggccgg tgttcttgtg gatccgccta
    14161 ccaaaaggac ttgatccaaa tccttggcag tcagtttggc atccgcaagg gcgcgtttga
    14221 ccggttcaat cgtccgttcg acgagatcgt gggtaagttc attgaatttg gctctagtca
    14281 atgctttttc aagatggaca gggccggatg ccgtcatcga aatgaacggc agggagattt
    14341 gggcagaggt aatcgagctg agttctttct tggctttctc ggcgctgtct ctcaatctct
    14401 ggagcgccat tttatctttc gaaagatcga cgccggtttc cttgcgaaat tcatccacca
    14461 accattcgac gatgcgctga tcgaaatcat cgccacccaa atggttgtct ccggaagtcg
    14521 aaatgacttc gaaagtaccg tcggagagtt ccaatatcga gacatcgaaa gtgccgccgc
    14581 cgaggtcgta gaccaaaacg gtttgttctt tgtctttctt gtcgatgccg taggcaaggg
    14641 ctgccgctgt cggttcgttg atgattctct tcacatcgag tccggcgatc ttgccggcat
    14701 ctttggttgc ctgacgctgg gcgtcgttga aataagccgg aacggtgatg accgcttctg
    14761 tcactttttc gccaaggtat gcttcggcgg attctttgag attacgcaga atcatcgcgg
    14821 agatttcctg tggtgtgtag tattttccgt tgacttcgac tttctgggtt gtacccattt
    14881 tacgtttgat cgaatagacg gtgttcgggt tagtgatcac ctgtcttttg gcaacttcgc
    14941 cgacttgaat ctcgtcgtct ttaaacgcaa cgaccgacgg ggttgttctg tttccatcca
    15001 aattgggaat tacttttgct tccttgcctt ccattactgc cacgcaggag ttggttgttc
    15061 caaggtcaat accgattatt ttcatatatt atcatccttt ccttatgcga cacattcgtc
    15121 gttagtttct atttcttctg attctttgtc ttctttgatt tccggtctca cattgataac
    15181 gacaagcgcg ggtctcaaaa gacggtcttt gaacatatat cctttcttga ccacttccag
    15241 gatgacgcct tcgggtttgt cggcctcata gcgtttatca atggcgtgtt caaggttggg
    15301 atcgaaattg tttccgacga cggtgttgtt ttgtttgacg ccttcatcag acaatacttg
    15361 gaagatggaa tccttgatca tccggaatcc caacaagaaa ttcttgaagt tcaaatcctc
    15421 cgttttcatg gaaagcgctt ggtcgaagac ttccaaatgg tcgatcaatt tgtccgccac
    15481 attcattgtc gcgtacttgc gatctcttac catctcgtct gtaagccttt ttttcaggtt
    15541 ttccatttcc gccagggttc tcaggtattt accgctcatc tcttccagat cgtccttggc
    15601 tttttccagt tcggcttcca tgttcttttt ctttttgccg tctttcggct ctttggtatc
    15661 tttgccgaaa gttgtttctt ccggttgttt ttcttctact tccggaagtt ctttttcttt
    15721 atcgttttca tccatctggc attattcctc ctcgtaaagt ttggcgatgt tctttgctat
    15781 gtatttcaaa agtggaatca cttttcgata atccattctg gtcggtccga caacggaaat
    15841 agttcctttt tcaccgtcta tcgtgtcata agtcgaagac acgaccgtgc aatccttcat
    15901 cgatgtaacc tggttctcgg tcccgatttt cacggatacg ccgtcagaat cggtttccac
    15961 cagtttgaag atatcattat tctccaccgt ttcgatgaat ttacgcaatt tctggatatc
    16021 attgaattcc ggctggtaaa gcatattcga ctgaccagtc agataatatc tcgtctgggc
    16081 aaatttcgag aaagcctcaa tgaacgagtc gacgatcgat tcccggtatt tcaggagttc
    16141 cttgatttgg ctgtgctgga tctcgtaatg gagtttctcg gaaacctggg agatcggcgt
    16201 gttgacaagc atttcgttca gaagatcgat gaccttgacc atttctgcga cttccatgtt
    16261 ttcggaaatt gtgatctgtt tcgattccac gtgaccgaaa ttcgtcacca ccaggataag
    16321 ggcctgggtt tctttcaaag gaatcatctg gactttcttg actttcgaat aataggcttc
    16381 cggtcccaag gatattgcgg tgcaattggt gacctggctt aagaggtcga tcgcttcttt
    16441 aatcagttct tcgcgttcga tttccttgtt ttcaaaaagc ttttcgaact caaaataccc
    16501 gtcggactct tcatcgatgt ttctgatgat cttttcgata tagtacctgt atccctgttc
    16561 ggaaggaatc cttccgctcg aggtatgggt cttttcgaga tagcccgcgt cttctaaggc
    16621 gctcatctcg ttacggatag tcgccgatga tatctccatc ttgaaagaca aagccttcga
    16681 accgaccggc tccgctgttt ttacatattc gtcaacaatc agtttgagta tggtttcctg
    16741 acgttctgtt aacatgcgac cacctctttt tagcactctt atcatttgaa tgctaagcat
    16801 attatacagg actgtgttag caatgtcaag aaaagagtgc taatatttta aggaaaaaac
    16861 cgagaggaat acgttttcat aaaaaaaccg gtttccagac cggaaaccag cagtaaatag
    16921 tatgaataat caaggcaaat atggattgtt ttcgatgatt tcaccgagcc ttttggattc
    16981 tccgtgtccg ggacaaacca aggtttttcg ggaaaacgac gttataagta acttgagcga
    17041 tttgttcatg gaagccatgc tgccggtata aagatcggtc cgcccgatgc catcggaaaa
    17101 caaagtatct cccgaataca aacgttcgtc gatatataac gaagccgaac ctgccgtatg
    17161 ccccggtgta ttgagataac ggatcacaat gtcttcgaaa acaagttctc cctgcatacc
    17221 gaacgtttct accgtttgtt ccttcggcaa ggaaaaatgg gaatgaaaag cccgggcata
    17281 gctgagtctc tcatcataaa gaaattgcag atcgttttcc tggatgtata ctctcgattt
    17341 tggaaaagct tcaagaataa cgggcaaatc ccggatatgg tcgaaatgtc catgagtaag
    17401 aaacaccgca acttcgattg ctccggattc tttcagacaa ttgacggctt cattgccgtt
    17461 gaagcccgga tcaatgacta ttgccgctga ttttttttgc aaaacatagg tattctgtaa
    17521 ggcgatattg tcataaatgc gttggtgcgt cattttattt caccagttgc atggcgccat
    17581 aaataccggc atccgatcca agcgttgcca aaacgattcc agctttgacg tcgaagggac
    17641 ggatataatc cggataatgc ctttttacgc tttgaagcaa gtaatcgccg gccaggctga
    17701 cgcctccggc aagcacgaac agatccggat cgattgttaa tgtgatggac ccgagcatag
    17761 ctgctaggtt gtcggcggct tcgtcgattg tcaacacagc cacttcgtct ttttccttag
    17821 caagatcgaa tactgtcttg gccgaaaaag aatccatgtt tctcagtttg ctggaaagat
    17881 cggtgttatc gagattatat tttgccagtc tggcgatgcc ggtagcggaa gccaccgtct
    17941 ccagacatcc ccttttaccg cagttgcaaa gaaaagcatg ttttttgtcc actactatat
    18001 gaccgaattc cccgctaatt ccatccacac cttcatgcag acgcccgtct atgatgactc
    18061 cgccgccgat tccggttccg agcgtcagca atacgacatt actataacct ttggcagctc
    18121 ctgcggcgta ttcgcccaaa gccgccgccg aagcgtcgtt tgtcaccttt atggcaatat
    18181 cttcgtaatt gaaatattta ttaagttcag tttttggatc cgtgattccc catccgagat
    18241 tgacgcaggt ttcaacaata ttgtttttca ccggtcccgg tacaccgagt ccgatcccgg
    18301 cgatcgatga aagaagccag ttttttttaa tgaaaacagc gatgtctctc aaaatatgcg
    18361 aaccgttctg gctacgatcg gtaatgatgc ttcttttatc taaaagaaag ccttccagat
    18421 tgaaaaggcc gatttttacc gatgttcccc cgatgtcgat tcctagcaaa tattccggca
    18481 tgtcatctca cctcgctaaa aaggcaataa gtggctttaa cataagaaaa agacttcaac
    18541 aagtgaagtc atttcttttc gcgatgaagt gtttccttgc ggcaacgcgg gcaatatttc
    18601 atgacctcca tccggtcggg atggttcttt ttgtttttgg tcattgtgta gttttcttcc
    18661 ttgcactcgg tgcaacgcat cgtgaattgt actcttacca ttaaaaaatc ctccattcaa
    18721 ctccttactt attttatatt ctgtaaggga aaaatgcaag taaaaaaaca ccaaaccgcc
    18781 gtattttgtc attttttggg cggtaacggg aaaaacacgg aagtacgatg cttgtattcc
    18841 tcgtactcgg gattgccctc gtaatgtttt tccaacaaag gaactcccga aacataaagc
    18901 agaagatagg tgatgatcag cggactcaag agcgaatacg ctccaaaatc actgagaaac
    18961 acaccgatga aaaggcccca ccagacgacg gtttcgccaa aataattggg atgccgggag
    19021 tatttccaca atccctctgt catgacatga cccctgcttt ccggattctt acggaatata
    19081 ccgagctgag catcgcttat cgtttccaaa gtcagaccta gtaaagatat cgccaatcct
    19141 atataagccg tgacgacgct tttttcggaa tcggaaataa acaccatggt gatgggcaaa
    19201 ctgacgatat aggcaagcaa agcttgaagc aggaaaatat tcacataggc tttgagaccg
    19261 ggatgtttgc cctcccattt ctttctcatg ttttgatagc ggaaatcttc tggcttgccg
    19321 tagtttcgca acatgacatg gacgaaaagc cgcaaccccc aaaggatgac gacgatgaaa
    19381 cataccgctt gggaaataag aatatgatcg gaagtttgga agcggtaagc tgtcgcgaaa
    19441 aaaacataga cgacaaaccc gaagccccag gcaatgtcga catatccgta attgcctttc
    19501 cgttttgcaa tcaggtaaat gatgctgaag tatgcgaaat aaagaataat tcctccgata
    19561 ataagccaca tatcacttta cctcccgact gaattccacg gcgattccga cggcgcctgt
    19621 gccggcgttt gcagctacga tgggcgatat ttcggtaatg aattcaggtg cttttccaca
    19681 aagttttgta attttttcag ccattttttc ggctctgttt aacgcgtcgg catgaatgat
    19741 agaatatttg acgatcggtg ctttttgtaa aagctttaga atcttttttt cattttgtct
    19801 gatgctaaaa gccttttccc tgatgattcc gtgtccggtc ccgtcaaggg acacgaccgg
    19861 ttttagatta agaattttgg cgatcatacc agtcactttg ccgacacgac cgccctttac
    19921 catgtatttc agggttttga ccgatacata tattcttgtc tttgatcttt tttcttccaa
    19981 tttcgccatg atttcttcga ttttgattcc gtcagaagcc attctggcgg cttccagggc
    20041 caaaagtcct tcggctccgg agttctggag cgaatcgaac actttcacct tggaatttgt
    20101 accggcgtat ttgcatagtg cgtcatatgt cccagaaaga gcgccggcca ctgtcacgac
    20161 cagaatgttt tcgtaatgct cggacaaaaa accaagcagt ctctcgattg tgccagaatc
    20221 cggttgggac gaggaaaaag tcgagtattt gtccatcatc tcgtagaatg tatcgatttt
    20281 taccgattgt ttgtcaagat aggagatatc gtcgactatc aggttcatcg gcaacatatg
    20341 gatttggtaa gtatccatca gatcctgcgg aatgtcagcg atcgagtcaa caaccacaac
    20401 tgtttccgga cgatccttgg ccattgcctg ttgctgcaat ttcatgtcgt cgattttttg
    20461 ccagtttaag cggccgtaag tacgtaaacg caagaatacc tgttcgggat tgtcggtatg
    20521 gatatgtaca tgcatctttt ccggttttcc cgagacgatc agcgaatcgc cgatatcgtt
    20581 cagagcggac ttgacagacg acaattccgt ttttccatcc aaaattaccg cttccgtaca
    20641 atatcttttt cccatgtcgt aatcaggatc atagatgata tcaaaagaaa catccgcttc
    20701 tttcagatcg gcagattccc tttcgcctgt ttccatgtat atggcgacgc cttcccaaaa
    20761 tgtcgtgaaa ccttcggctc cggcgtccac aactccgttt tttttaaggc tttccaattc
    20821 gtcaggagtt tcttttaagg ccgtttttgc ctgttcgaga ctggtccgaa aataggtttt
    20881 gaaatcggta agacgttcct tgatatcaaa tgctttccgc aatacagtca ggatggtccc
    20941 ttcgaccgga ttcatgatgg cctgatagga acgaaaaatc gcatatgata tcgcctggcg
    21001 aaaatcctca agtctggctt gacgaagatt tttcagtttt tcataaagcc cgacaaaaaa
    21061 ctcggcaaat ataattccgg aatttccacg ggatccgtcc accgcggcgg tcgcaatgga
    21121 agcggcggtc tccgaaagat cttccgatga tttggctttg tcgatgattg attgcatgag
    21181 aaatgacatg ttcgtaccgg tatcgccatc gggcaccggg aacacgttga tggaattcag
    21241 atatttcttt cggctgatca gccggcgggc gccggctaga aaagcctgat acaggtcatt
    21301 acctttcagg gttataagtt ccacttgcct tttacctcgc tatcaaataa gttacggttg
    21361 atacaatacc acccaaaatc atcccccagg caatatcaat caaaacaatt tgccacggcc
    21421 agtccttcag agtggccata ttggtcaaat catacgtaga atatgcaacg aagccaaaaa
    21481 acatgcttgc gtaaatggcg taatttagcg attctctgcc aacagcaggg taaatgacaa
    21541 aaaatacaag tcccgccacg aatacaagat agaagattat ggctggtatc cattttacct
    21601 ttcctatata accgccaaga taacgatcat agactttttt ggccactaca cccagccaca
    21661 acatgtcaat aacaagaaag aaaccaaggg caatggcata cagtttgaga tatgtcatgt
    21721 ttacccctcc ttaaaatcat tctagcatgg atttgtgttt ttataaagaa atgtgcaata
    21781 aataaaaggt gcttatatga tttggtaaaa caaaaaatgc ttgcatgtgc aagcatttta
    21841 gtttgttaca gtgttcttac tcgaagaacg cctttcattt ttttagaaat ctcgatggcg
    21901 gcttcgtttg tttcggcgtc aaaatccaca acagcgacgc cataaccgtt cttggcccgg
    21961 tttatcaggt ttccgagact gacgatgcct tccaaagccc tcgtcaggct gccaaggaat
    22021 tcctcgctgt ctttgtaaag gatctggatg cggtatttgt tctgcttgcg tccgggatca
    22081 agagcgggaa agttcaccga gttctttatt gaaccgtgtt cgacgtaacg cattagttcg
    22141 tcggttgcca tcatcgcgca attgtcttcc gactcttcgg tggaagcgcc taaatgcggg
    22201 attgcgatca catgattcat attggcggtt ttcggattgg gaaaatcggt aacataactt
    22261 cggacgattc cgctttcgat agagatggcg agatcgtcgt cgtttaccaa ctggtcacga
    22321 ctgaaattta aaagaacgac acccggtttc atgatggcaa aaacatcttt tgcgatcata
    22381 tggcgggttg aatcaagcaa tggcagatgt aaagtaatat aatcgcaggt agcataaatg
    22441 cggtcgagtt cggtcgtgag ctccacttcg tttggcagtt tgtccgcatt ttccggtgaa
    22501 agataaggat cgtatccgag aacggacatc cccagacaga tagcggcttc cgctactttt
    22561 cccccaacag cgccgagacc gatgactcca agctttttgc cgataatttc ggttccgccg
    22621 aaggctgatt tggctttttc caccgacttg gacaagttgg cgtcctcttt gttatccctc
    22681 acccagttca ctccgccgac aatatcccta gctgccaaca acataccggc gattaccagt
    22741 tccttgacgc cgttcgcatt ggctcccgga gtgttaaaga caacgatccc cttgctcgca
    22801 agatcggcaa gaggaatatt gttgacgccg gcgccggctc tcgccactgc caataatttt
    22861 ttcggcaatt ccatttccag catgttgaaa ctgcggacta aaatcagatc ggcatcattg
    22921 acttcttctg tcaattcata accgttcgtg aatcttgtca gtccgaaggg agatatctta
    22981 ttcaggcaag ctactttcat ttggcatttt ccttttcaaa tttcttcatg aaatcaatta
    23041 gactctcgac gccttcgatg ggcatggcat tgtagatcga agcacgcatg gctccgaccg
    23101 agcgatggcc tttcaggttg tcatagccgg ctgctttggc ttccgccaca aacttggcat
    23161 ccatttccgg gttaccggtt ccgaaagtga cgttcatcaa agaacggtcc ttcttctcga
    23221 cggatccatg gaacattttc gaatggtcga gaaaatcata tagcatgctg gctttggcga
    23281 tgttgatctc ctgcatcttc tcaaggccgc cgagattaag caaccatttg aaaacgagac
    23341 cgcagatata gataccatag gtaggcggcg tgttataaag cgagttcttt tcggcatgga
    23401 tatgatactt cagcatagtc ggcacgaatt ctggcaaatc ggttctgata aggtcgtcac
    23461 ggattatgac gattgtcgta ccggccgggc cgatgttttt ctgggcaccg gcgaaaatca
    23521 gtccgtattt ggtgacatcg atcggctccg acaaaatgca actggacata tcggaaatca
    23581 agacttttcc tttggtatcc ggttctttgg cgaatttggt tccgtagatg gtgttgttgg
    23641 tgcagatata gacatagtcg gcgttttccg aaatcggcag gttgtcgagg tcgggaatat
    23701 aggaaaacat tttgtcttcg cttgtggcga tcttacgggc atccccgtat tttttagcct
    23761 cgttgtgagc ttttttggcc cattcgccgg tgacgatata atcggcgacg ccgtttttca
    23821 tcaaattcat cggaaccatg gcgaactggg tgtgtcctcc gccctgaagg aaaaggacgt
    23881 ggtagttgtc gggaatggaa agcagggtcc tgagatcctt ctcggcatcc tggatgattt
    23941 ggtcgaacgc tttggagcgg tggctcatct ccattacgga catgccggta tcgttatagt
    24001 cgagcatttc cgctgcggct tgccgcagga ccggttccgg caatacggcg ggtccggcgg
    24061 agaaattaaa tactcttttc attatctatc cttcctttcg ttatcagacg gcattcgaga
    24121 taataacgtt tttctatggc ttcgcccaga gaaaacgatt ttctcggtaa tactccgttt
    24181 tcgttaatgt agggaaacag tttgtccctt cccaatgggg gcaggactaa tccgatcgat
    24241 ccgggatttt ccgctattat tccctcaagg tttttgaacc cgtggacata gtcgatcctg
    24301 gtatccggat gaaatgcgag ataatcatca agaaagtttt ggatccatac gatggtttcg
    24361 aacggacttt ccggcgacaa tatgtaatcc tcgccttttt ttgtcaatat cttcatcgga
    24421 ttttggccgt agcttgcgcg gctgagatct tcaagaaagt catcgccagg tctgtatatg
    24481 acccggtgaa tcggttcgaa agcgagtcct ttgtcataaa tgctttcaag ttcgacgagg
    24541 gcgtatctag ctggatgccc gacaagttct tcttccggaa gattcttctt cagattttcc
    24601 cagcagactt tggcggaagc cagagagtga ttgccgtcgc cgacgatcaa cggtgtcttt
    24661 tcgatttgtt cattgatttt ggcgagcagt tctgcgcttt tttctaacag atgccccgtg
    24721 atatgtccac cgcccatatt caaatcgaaa tcgtatagtt ttttcatgtt tctggtgtcg
    24781 gaggctgttt tggcgatcaa gccgtccaca tcatcgaaca aaaccagaat gtggggaagt
    24841 tcgatcggag cattttccct gattttgacc cggggaggaa tccgatcgag aaccgttttt
    24901 tcggaagcga cgatccggcc ttttttaccg ggcatgtatt cgtattcttc taagtcgatt
    24961 gtcaataaca agccgtaacg gtgttcatgg taaggcgttt tgcgatcaat gatgatcgga
    25021 tttgatactg tgtcgaagat gttcgagcga agatataaat tcatgttgga gttggcgctt
    25081 tggattctgg tttcaaatcc gagatgcaga tatgcctccg gcagaatcaa atgatacgta
    25141 gaaggaacgt cttccacttg ttggcggaga cgttcccagt acgatatctc actcgtaaac
    25201 tggtcgcaag cgataaccga ccataatgtg tgatcgatcg attgcttggg cagcagaatt
    25261 tctttagatg agacaacttc catagatcaa tacacgccct ggtccaacat ggcctgatat
    25321 acacgcatga atccggcgat gttggctccg atcaccaggt tgcggtggtc ggtgtatttt
    25381 cttgcttcgg tataggcttt gttgaaaatg tcggccatga tgacttcgag tttctcgtcg
    25441 acttcttcat atgtccaagg ataatgcatg gcgttctggc tcatttccaa accggagacg
    25501 gcaacgccgc cagcgttggc tgccttgcca ggagcaaaca gaatgtcagc atgctgcaga
    25561 tatttcgtgg cttcgatcgt tgtcggcata ttggcgcctt cggcaactaa gaaacatccg
    25621 ttttcaacga gagctttcgc accttctaac ggcagttcgt tctgagtagc gcagggaagg
    25681 gcgatgtcgc atggcagttt ccaaatttcc gtcggattac tgttatagac ggcattggaa
    25741 cggtattcca gatacttggt cgagaattcg ggcatatcct tcgtgagcat cttgacgatg
    25801 tcgatatcga tgccgttcgg atcgtagaca catccgttga catcggacat ggcaacgact
    25861 ttggctccga gttccctggc tttcaaggcg gcatagagac cgacctttcc ggatccggag
    25921 atgatgatgc gtttgtcttt catttcgtcg tttctgtaga cgcgaagcat ttcttggacg
    25981 aagtagcaaa gaccgtagcc ggtagcttct tttcttacaa gcgaaccgcc ataggtgaga
    26041 cccttgccgg tcaagacgcc ggtcgattcg ttttgaattt ttttgtacat tccgtacata
    26101 tatccgacct cgcggaatcc aacgccgata tcaccggcgg gaacatcggt gtcgggacca
    26161 atgtggcgat agagttcaag catgaagttc tggcagaaac gcatgatttc atcatccgat
    26221 ttgcccttgg gatcgaaatc ggctccgcct ttgccgccgc ccat
//
LOCUS       DAOPJL010000017        25096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_2086_length_25096_cov_8.400663, whole genome shotgun sequence.
ACCESSION   DAOPJL010000017 DAOPJL010000000
VERSION     DAOPJL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 25096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25096
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_25096_cov_8.400663"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLP02_03405"
     CDS             complement(<1..371)
                     /locus_tag="PLP02_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HPF42531.1"
                     /translation="MNKIPFGPTYEEMLDPSLLGEELRNKALEAKKNELDPLNLFNIT
                     WKNENNEVNKIVLPKELTGVECNIVVLLGKYFPSGSHKVGPAYSTLIEGCVDNAIVPG
                     EHTILGPSTGNFGIGVSYICNL"
     gene            complement(368..1621)
                     /gene="thrC"
                     /locus_tag="PLP02_03410"
     CDS             complement(368..1621)
                     /gene="thrC"
                     /locus_tag="PLP02_03410"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPF42532.1"
                     /translation="MSLENISGFVCTICNRKYPQTFTGLTCPHCGEKGILDVTYDYES
                     IGKQISRESLKADNNQSIWRYLAYLPVRETDTIATLKVGMTPLYKAVNLGRLLETENL
                     YIKDEGLNPTQSLKDRASIIAVVKAKETNAQTIACSSTGNAASSLAGNAAKAGLSCVI
                     FVPKRAPAGKLAQLLAYGANVIRVDGDYKAAFDLSKAAIEKYSWYNRNAAINPFLVEG
                     KKTVAYEICEQLDFQVPDWIVVSVGDGCTIGGVYKGLCDFSSTGMIDRIPKLLGVQSE
                     GCDPIAKAFWKNGPIEPEEENTIADSIAVGIPRNPVKAMRAVQKTGGAFIAVSDKEIM
                     DAMQLQGKTEGVFSEPAASAATAGLIKALKTGVIQKTEKIVVISTGNGLKDTKHALEA
                     LPEPVLLKPDIEAFAKYMKNKKEIS"
     gene            complement(1617..3335)
                     /gene="ade"
                     /locus_tag="PLP02_03415"
     CDS             complement(1617..3335)
                     /gene="ade"
                     /locus_tag="PLP02_03415"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPF42533.1"
                     /translation="MDKEKLRRVALGIDKAAIVLKNCKIVNVFSGLIETGDIAIEEGI
                     IAGIGKYEGITETDMCFSYVSPGLIDGHVHIESSMLSPGQFAKVIVPLGTTTVIADPH
                     EIANVCGLSGIRFMLESSANVPLDVRIMIPSCVPTTVFETAGASINADEIGALKNEKS
                     IVGLGEMMNYPGVIGGDRETYRKLYVMADKMIDGHAPGVTGEELNAYVLAGVKTDHEA
                     TTAMELREKVSRGMYVHLREGSVAKNEETLLKAVNKDNLSRLMFCTDDKHPEDIRKEG
                     HINHNVTMAIENGLDMVDAIKMATINTATCYNLKNTGGIAPGYYADLIVFDDPGKIVP
                     RLVYKKGQLVARDGKALFSVIPTDDPAVEKTVRFDPERLNFSIPLHKNRVHVIGFDKD
                     NLTTRKLTENVRVENGYYRQEKNSGILKLAIVERHRMTGNIGLALVRGYGLKGGALAM
                     TIAHDSHNMVVIGDDDEAMRKAALKLREIQGGIALVYGNEIFDYLQLETGGLMTQKDY
                     HYVEQKLERMSKQARKMGVDPNIDDPFLALAFLSLPVIPELKVTDKGLFDVNKFKIIP
                     LEVMED"
     assembly_gap    3354..3453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3454..3840)
                     /locus_tag="PLP02_03420"
     CDS             complement(<3454..3840)
                     /locus_tag="PLP02_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPF42534.1"
                     /translation="MVKQVISETLKEALELMQQEDYILVSGGTDLMVQNYSESGIVPD
                     FKRPVMYLANLEELKQMKVEKDEIIIGAEVTLETIRQSSIIPDILKEIISEMASPGIR
                     NLGTLSGNIGNASPAGDSLVGLYLLDA"
     gene            complement(3834..4307)
                     /locus_tag="PLP02_03425"
     CDS             complement(3834..4307)
                     /locus_tag="PLP02_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPF42535.1"
                     /translation="MAEIEFILNHQKIVSETDPSLRLLDYLRNTLAMSGTKEGCGEGE
                     CGACSVLLDGKVVNSCMVPMANVAGHEVTTIEGFRETKRYKILEQAFVQCGAVQCGFC
                     TPGMIIASESLLRQNPHPSESEIREALSGNLCRCTGYNMIVDAVKKASEDGDGLW"
     gene            complement(4294..6390)
                     /locus_tag="PLP02_03430"
     CDS             complement(4294..6390)
                     /locus_tag="PLP02_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPF42536.1"
                     /translation="MSDINKTIERVDAREKISGIAKYVDDLKMEGEIYAKTIRATIAK
                     GKITDISVPPLPEGYATVGVKDIPGKNFVKIIFDDQPVFTESEVTYFGEPIMLLVGPD
                     KAVLRKIANEIRIAYKEEKPNLEWDDPMISYHFSKGDGDQPFQTASKTFSDVYITGYQ
                     EQAYLETQGLIGYPEGNRIVITGSMQCPYYIKNALINCLGCKDDMVRVIQQTVGGAFG
                     GKEEFPSLMACQLAVAVYKMKKPVRLIYDRKEDMPVTTKRHPSRIRLEAAINKKNEIT
                     AIRAAVGLDGGANAGLSGVVLQRAMIASSGAYTFPNMDVTGRVYRTNTVPNGAFRGFG
                     APQMLFSIEMFIGHVAKFLGLDPFEFRMKYLAKQGDKTSTSGTFRDPIIMPELIGKAM
                     KESDYLRKAQIYSAGNGYQGIGMSFFLHGCGFTGSGEATHIKARVRLEKDENDNVRIL
                     IAAVDMGQGIRTTMRKLVASILEIPMEKVIFDLPDTDFVPDSGPTVASRTMMIVGGLV
                     DRCARKLKEKWLANEKQILEENFQQPEYVKWDEEKLAGDAYPAYSWGVNIVEVEIDKA
                     TCQVKPIGIWSEYDLGHAIDEMVIKGQCDGGIAQGVAYGYLEVMEQKDGRLRQKNLTD
                     YIIPTAVDMPPTMSGVIDNPFALGPYGAKGAGELTLVGGAPAIAKAIEMAIKRNVTEI
                     PVTPEKIRELIDNGRN"
     gene            complement(6383..7486)
                     /locus_tag="PLP02_03435"
     CDS             complement(6383..7486)
                     /locus_tag="PLP02_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPF42537.1"
                     /translation="MDLKTTFDGLELENPLMVASGPLNGDDKKIAFFASEGVGALTTK
                     TISTKEPMIPKPCIYGGYDHVLNSELWSEYPLEKWTREFLPAIVKGKDRPLIVSVGYS
                     EEDMETLIPELDRFADAFEVSTHYVGTDLDKIRHIVETIKKHTEKPVYMKISPHLPDP
                     EGFAKTIREAGASGVVAINSLGPSMKVDTDKRSTAYGNASGYAWLSGPAIKPVALATV
                     YRIKQAEPSLTVIGVGGIAKADDVLEFLLAGASAVQMLSAALIKGKQLYRQIIAALPA
                     ALEKHGFSSIQEVVDTQLTNTVSFTANFPAWQKDGCNRCRICEKACPYFAISYLEGNI
                     LFDHTKCSKCGLCAAICPAGVITFIKNGGMNGE"
     gene            complement(7477..8790)
                     /locus_tag="PLP02_03440"
     CDS             complement(7477..8790)
                     /locus_tag="PLP02_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF42538.1"
                     /translation="MAKLLINAMLYDYDRFIPDGFLLFDEQIIEAGPMEKLPQGDHET
                     IDAGGMLVLPGMACGHTHLYSAFARGLDLKLDIKNFKDILEQLWWRLDRGIDNRIAYA
                     SALSFGAEMLENGITTICDHHASGKEITGSLEAIKKAVVNDIGMRGVFAFETSDRFDV
                     AKAVAENRDFAKNNRTERAAGTFGMHASMSMSKETIRLVKENLDGETIHVHVAESKYD
                     TEDCLEKYGMSIIDRFDNYGLIGKDSLIVHGVFLNDSELSLLAKKDAVVCLNVSSNMN
                     NGVGLPDIKNMKAKGVKVILGNDGLHAGMALEYLNLYYAMHHKYGSLTGLSSDFVKDA
                     INETYDYLSRKLGISLGKFANGFAADFIIVPYRPATPIDSGNAMGHLLFGVYPGFSPK
                     DVYVAGKMLVKDHELVNRSKKTEAIIKKAASDLWNNVNKEDEPWI"
     gene            complement(8791..10095)
                     /locus_tag="PLP02_03445"
     CDS             complement(8791..10095)
                     /locus_tag="PLP02_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF42539.1"
                     /translation="MVDLAIMNGKVYQDGDFVDANVYVDKDIFQAVTAETYPAATEVD
                     AKGMLVFPGLIDPHVHFDLDLGVMRSVDNFERGSLLAALGGVTTIIDFLDPADTPADL
                     EMKYRRRVFEAEDSKVDYMFHACIKEPHCDLESFVIKMMSLGMNTLKCFTTYSDSQRM
                     INDNEIIELLKLSEKYHFLLMVHVEDDDLIDLNPSYTFRELGISRPKQAEIQKALQLA
                     GYVTRYGGFLYMVHVSSGETIENLKEYYPEILNRRFFLESCPQYFLFDDSVFSQKDGY
                     LYTTAPPIRSKTEKMYLNANIDWIYAIGTDHCAFTKSQKHGELLSEIPLGIGSIEHSF
                     DVMYNLYGNKIVEKMTTHPAQMNGLAGRKGKIKAGYDADLFLYRPGKGKIAEDHSGSD
                     YSVYMGVQKAGTVVSTLSNGSFIVRDREFENGFGRLLKGGRL"
     gene            complement(10111..10578)
                     /locus_tag="PLP02_03450"
     CDS             complement(10111..10578)
                     /locus_tag="PLP02_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPF42540.1"
                     /translation="MVSIVTGKINSGKTGRMISIYEKTHKGDGFVSLKNMDGNTVLGY
                     DAMQLSTGKKFPLVSRKGRNSVKFRQACRIGPYFFSQDAIDMIGEKIGEMIKNNVSPL
                     YLDEIGPLEIEGKVFDNTLKAMISSGLDTCITVRTGLLEKVIERYDIGEYRIV"
     gene            complement(10556..11413)
                     /locus_tag="PLP02_03455"
     CDS             complement(10556..11413)
                     /locus_tag="PLP02_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPF42541.1"
                     /translation="MFSPLFDMADFYESLLELKRIGIPLMVVVAVAKEGAGPVDVGKK
                     MVVAEDGTAFGTVGGGAIEFYAKKKCVELMQKHENLLETYLLSEGKIIPETKTLPMAC
                     GGRVTLFYEYVGPKEFVYIFGAGHVAKALGYVLRTLGFYLIVIDDRQDVLDDYPYASE
                     KHANQFVPFIDEVGIRENSMVVVSTPSHEYDYHVLNRILEKKIQLKYFGMLCSPSKLD
                     DYLENTYKAFGNDLDLSDFYAPIGLDLGGNTPEEIAVSIASEMLAVSNQRPSGMHMRE
                     TRHGKYRYW"
     gene            complement(11394..12059)
                     /locus_tag="PLP02_03460"
     CDS             complement(11394..12059)
                     /locus_tag="PLP02_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42542.1"
                     /translation="MKKNFWQRLSVILMFGSVWGILEASLGYVLHLLPALIAGSVMFP
                     IASVILLWAYKSSQKKSDLVWIGFVAALIKGIDFFLPNLSYFKVMNPMIAIVFETLMV
                     VAVIPLLEKSNLFSKICAMPLAALGWNLLFLNFHAIYYIVNLAVSPDTAYLNYNISSV
                     ANFLSYMQNNFLLSGILAAGLVFLFTYLVFPKMKPTTHVAYWLSTSTFALGLALTVVF
                     TII"
     gene            complement(12056..14374)
                     /locus_tag="PLP02_03465"
     CDS             complement(12056..14374)
                     /locus_tag="PLP02_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPF42543.1"
                     /translation="MKVVNNPVPSVDGKGLMMGRHGYTDDFAEKDSLVIKILRSPHPY
                     AKITKIATEKAKDVPGVTMVLTYKDFPRIPFTRAGQGYPEPSPHDKFVLDEYVRYIGD
                     EVAAVAATSEKACIEALKLIDVEYEVLDSVLDFEKAIDNQSVIHPEPEIHEMFPIGFE
                     PKRNIAASYEMEVGNVEEELEKCDVVIRDSFLTQAQAHAMMEPHAVNARMDYQNRLVI
                     YSSTQTPFHVRRIISQTLGIPLAKIRVIKPRVGGGFGGKQALHGEMFASYVTWKTGKP
                     SRIVFTRKEVFESTYTRHPMKIDIAIGAMNDGSMKAIDCKVLSDTGAYGEHALTVFMV
                     TGSKVLPLYNKVSAVRFGGKVVYTNHTPAGAYRGYGAIQGNYALEAAVDILADKLGMD
                     PIVLRQKNMIKEGETSPIFAIMGEGTEGTAMTMDSCKLDYCVKQGKKLIGWDNKYPRY
                     EETEDKVRSVGMAIAMQGSGIPLIDMGSASIKLNDGGFYNLMTGATEIGQGSDTVLSQ
                     IAAEELMTDIDKVVIYSSDTDLTPFDTGAYASSTTYVSGNAVWKAARKLKEMMIEAAG
                     EFLDGGLDTIEFDGKTFTNIKNGKTIDLVTLSNKLTYGERQRQLQATSSYVGHKSPPP
                     FMAGFVDIEADKKTGEIKILDYVSVVDCGTPINPNLAKGQVEGAIVQGIGMAIYENVT
                     VTSKGKLVSSDFMNYRIPTRKEIGKLTTEFAESYEETGPFGAKSVGEIGIDTPPAALS
                     NAIYNALGVRMKDLPMTAEKIYYAMKKKETEK"
     gene            complement(14371..14823)
                     /locus_tag="PLP02_03470"
     CDS             complement(14371..14823)
                     /locus_tag="PLP02_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPF42544.1"
                     /translation="MRIRIVVNKEEKHFDVDLNEYLVDTLRRNNYLSVRRGCDTTNCG
                     VCTILLDGKPVPSCSLLSVAVNNHEVTTVEGIMEEAEKLAEFFGHEGADQCGYCNPSL
                     ALTVYAMKRELKNPTPSEIKNYLVGNLCRCTGYQAQEIAIEKYLGDKS"
     gene            complement(14813..15616)
                     /locus_tag="PLP02_03475"
     CDS             complement(14813..15616)
                     /locus_tag="PLP02_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPF42545.1"
                     /translation="MIIESFFRPTTLDEAFAKYMESPKNQILGGGAWLKLTGSRINTL
                     ISLEGLGLDKIVNTEDKVIIGANATLRGIETDTAIKNISGGILSMAMASVMGVTLRNI
                     ATIGGTIMGRYPFSDVITALLALDATLVFYSQKNMKISEYLNQEKPEKDLLMYVQIDK
                     SPGYGYFHRVARTSTDFAIVNLAIVKTGNIWRIAVGARPGIARLCPLAAEALQKDQDH
                     TKENLAAVAQMAVRELEFLSNNKASGEYREMLARTYVERGLEATCTNAD"
     gene            complement(15618..16187)
                     /locus_tag="PLP02_03480"
     CDS             complement(15618..16187)
                     /locus_tag="PLP02_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPF42546.1"
                     /translation="MAEAVILCGGYSSRIGENKMMLELAGHPLIWHTVAGVSRVTSRV
                     IIVTGHYHDDISKLFSGWDNVVIIRNTDYEKGMFSSVKCGVRQTESDFFVLPGDHPLV
                     KEATFKLLLSGTKDVRVPAYAGRKGHPVFFSRRLKEALLDEPDDSNLKIFRNHYDVEI
                     FETDDEGIIKDVDTLKDFFYIQNLHEEER"
     gene            complement(16326..17900)
                     /locus_tag="PLP02_03485"
     CDS             complement(16326..17900)
                     /locus_tag="PLP02_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPF42547.1"
                     /translation="MNDEMEFCLLFTSDVHGHLYPIDYVTEKKASNSLANIASVIRKH
                     RIANTVVLDLGDILQGSPMMDYHWHKARNKANPAAFCLKGIGYDYFVPGNHDFNYGRA
                     YLSRFISQITAKPLCANILTKKGNPAIGSPYDIVELTEGLKIAIIAVTTQYIPNWEKK
                     ANVRGLVFTDACLQAKKLVERIRKEEDPTMVIVAYHGGFEKDLDTFLPYVDDTGENEG
                     SRMLETIEGIDVLLTGHQHRKICQKVKNTWVIQPMSNALAVGKVITKFAKNGDKWGIT
                     GIDAEIMESTGYPENVEICKRLEKEQIATEEYLDKVIGIAPKNDMEIKDQFDARLHKH
                     KVVTFINRVQLDKSKAMITCSSLGNSVTGFKKMITVRDVYTTYLYPNNLVVVEITGEK
                     LRLALEKCAEYFVVENGRISSNPKYSHPKVEHYNYDMFDGIDYTIDISKPEGSRIVRL
                     ERNGVPVRENDILTLAMNNYRYAGGGEFAMYKGLKTLRSYGDDITDLITKSIEKKHLI
                     VVDDPKNIVVKASKNQ"
     gene            complement(17981..19147)
                     /locus_tag="PLP02_03490"
     CDS             complement(17981..19147)
                     /locus_tag="PLP02_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPF42548.1"
                     /translation="MAGILKLSGNILVESMDFGPAITAFFIDKFTDMPPIERIDVNNV
                     SESGTLSSFSTYRCVDGTVVTFDSYPDQNFLSPMDFLEPREKNELKKEIVFKIRLKES
                     FTYDNQEISLIEVNIQKKIMAPSIAMFSDGVIEFDRKIAMDYSFYMPKSVGPVPLIVW
                     LHGAGEGGRSVWRRLLKPPVSRLVEPHIQNIFGGVAILIPQAETFWMDDGKGNYTVDG
                     TTMYKKALHGLITGILRSHPRLDPKRVYVGGCSNGGFMTIKMLLSYPELFAAGFPVCE
                     VYNDRWLKDEDIRVLSTIPLWFVQSRDDPVVRPEDFCLPTCKRLLETKSNDIHLTLIE
                     HVTDQTGKYTLHNGAPYRYHGHFSWITALNDWCVDVIDGKKTTMFSWLSRQRKP"
     gene            19311..20558
                     /locus_tag="PLP02_03495"
     CDS             19311..20558
                     /locus_tag="PLP02_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42549.1"
                     /translation="MCDTLYRKTGTSAFFAKNSDRSPNEPNLTVFVEAKPGDPLNRKK
                     CTYVEIDENPDTFAMILVKPSWMWGAEMGINSQGVAIGNEAVFTKNRDKKTERLTGMD
                     LLRLGLEEGDTARHALDVIIGYLEKYGQGGNCGFDKPFYYDNSFLIADSREAFILEIV
                     GKKHIEWQLDASGNISNRLSVMNETAAAFFKRNTEPVFTCFSGSFNREKTLREAHITG
                     IKDVIKAMQMHPAGLTTKTLFRRGTLKSPCMHYSTLGDHTTSSMIVEWKGDKITIWLT
                     GRSTPCLAIYLPVIFDRPGIPVFKSEKESLDFWLEREYLSRAVFSGLVIERDYKIQTN
                     AIEEKLIQMAAGLGDDLSFEEIGAFNNYVLEMEAGFVKEYANIIHKVQSDPSVLTGRW
                     KKKTLTLGKNVFLASLEERRNTR"
     gene            20555..21673
                     /locus_tag="PLP02_03500"
     CDS             20555..21673
                     /locus_tag="PLP02_03500"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPF42550.1"
                     /translation="MKIVLAPDKYKGALKSIQVANAMEKGIRKVLPDAKIVKLNVSDG
                     GEGLADAILACRKGRKIVLRTTGPENKEVTSSYAFLEDMTAVIELAESAGLVLAKNPN
                     PAKTTTYGTGEMILDALDRNAKEIWLGLGGSATNDAGCGIASCLGVKYFDKFGRRFVP
                     TGDTLVKIAAIDTSEIDERIRNIKIICLTDVDNPLYGPNGAARIYARQKGADDAMIDL
                     LDENLEHYAKILQDSFGFDANFAKAGAAGGTPVSMKAFFHASVRNGVLFLLEECGFGQ
                     VIKDADLILTGEGRFDHQSLQGKAVSGIMRKASEAGVSVYVLAGSVEKNLLLYKPKEI
                     AGLIQVTPDDMNIGEAIKNSETLIEASAENLMIKLIKK"
     gene            complement(21705..22196)
                     /locus_tag="PLP02_03505"
     CDS             complement(21705..22196)
                     /locus_tag="PLP02_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HPF42551.1"
                     /translation="MKRYYLKQKVLALKDTYSVFDESGKEVFHVKGAWISFTRRKDFC
                     EPTGEILFTIKRMLFHLLPHYSLTDSSGKEVANIKKRFTVLSHRIDILSSRGDYSITG
                     DFMAHDFTIEKAGQAVVEIHKKWMSWGDSYEIGVSDEENASFYVALVVMIDDCLYNRK
                     NHR"
     gene            22399..23370
                     /locus_tag="PLP02_03510"
     CDS             22399..23370
                     /locus_tag="PLP02_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPF42552.1"
                     /translation="MKPIFLKKALLAFMILPLLFLSGCDLFTTGDASTDTSDSIINVY
                     GIDESVLESLATTNDYPSTVTAAAIVMPSTVEITAEISFSYTASYYSPWGSRTETVTD
                     SAISEATAFFINEDGYLLTNAHVVTLSDYEDYPGFTYTGWNITLNYADSDTIFSASIV
                     EYDEALDLAILQIDEPIADLAYVTFFNITSPDDSAYGTSEAISLYYGEPIIAVGNAEG
                     YGISVTSGVVSAPLRYFDDSGTVIQAIQTDAAINSGNSGGPLSNMWGATIGIDSFKIV
                     TSTSENLGYAIPSYVVMDYIDSVSDSIQYYYTNARSFDKTDVISHLG"
     gene            complement(23444..24658)
                     /locus_tag="PLP02_03515"
     CDS             complement(23444..24658)
                     /locus_tag="PLP02_03515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF42553.1"
                     /translation="MLNEKEKSLSVKQGFKNIVLLGLVSMFIDLSTEMVYPLVTIYLS
                     TIASVAIIGVVEGLAESVAAILKSYSGYLGDKSGKKKILAFTGYFSAVIYKILLFFSF
                     NWVFVLIAKLVDRIGKGIRTAPRDALVAESGQATLGRAFGLHKALDMFGSAAGVAVSI
                     LLLSLLVKNGETSADMAVFKKIFLFSAIPALFGLFCLLFVKETKTIGKAIKESGPGSF
                     RPEKRLVAYLVVVFLFSIGNSSNAFLVLRLNAAGYSTIDVLLIYLVYNLVSSLSSYPL
                     GKLSDKIKRSRLIIPAYLLYSAVYLGVAMNVDKLGFYLLFGFYGIFQSLISGAERAYI
                     VEISQPEYKGTVLGLYGTSQGLGLLFASLIAGLLWGIGMEYTFLFGAVMGVAAALMLF
                     FVFRMKTKEKTT"
     gene            complement(24687..>25096)
                     /locus_tag="PLP02_03520"
     CDS             complement(24687..>25096)
                     /locus_tag="PLP02_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42554.1"
                     /translation="RRTSRKMLELVLVFITAVVMTLLIEFPFYWFGKYRDKLFVLGFL
                     LINIATNLAVNTVIYWFVYRSNLTAGPLSLFLSNVGKEEIVLFVSLEITVFLIEYMVL
                     SQLASRGTKLFLTVFFANLTSAFLGTVLLWLIF"
BASE COUNT         6013 a         6260 c         5894 g         6829 t
ORIGIN      
        1 agattgcata tgtatgaaac gccgatgccg aaattcccgg tcgacggccc caatatcgta
       61 tgctctcccg gaacaatcgc gttatccacg catccttcga tcaaagtcga ataagccggt
      121 cccactttgt gggatccgga cgggaagtat ttgcctagca atacgacgat gttgcattcg
      181 acgccggtga gttctttcgg caatacgatt ttgttaactt cgttgttttc gtttttccag
      241 gtgatgttaa aaagattcag cggatcaagt tcgttttttt tcgcttccag agccttgttc
      301 cgcaattctt cgcctaagag cgagggatcg agcatttctt cgtatgtcgg tccgaatggt
      361 attttgttca tgatatctcc ttcttgtttt tcatatactt tgcaaatgcc tcgatatccg
      421 gtttcaacag gacgggttct ggcaatgctt caagtgcgtg tttggtgtcc ttgagtccgt
      481 tcccggtcga gataacgaca attttttcgg ttttttgtat tacgccggtt ttcaaggcct
      541 ttatgagacc ggcagtggcg gcggaagccg ccggctccga gaaaacgcct tcggtttttc
      601 cctgtagttg catcgcatcc atgatctctt tatccgacac cgcgatgaat gcaccgccgg
      661 ttttctggac agcccgcatg gctttgacgg gatttctggg gattccgacc gcgatcgaat
      721 cggcgatggt attctcttct tctggttcaa tcggtccgtt tttccaaaac gccttggcga
      781 tcggatcgca gccttccgac tggactccga gcagtttcgg gatcctgtcg atcatgccgg
      841 ttgaagagaa gtcgcataaa cccttgtata cgccaccgat cgtacacccg tcgccgactg
      901 acacaacgat ccagtccgga acctggaaat ccagttgttc gcaaatttca taggcgactg
      961 tctttttccc ttcgaccagg aaaggattga tggcggcgtt gcgattatac caggaatact
     1021 tttcaatggc tgctttcgac agatcgaaag cggctttgta atctccgtcg acccggatga
     1081 cattggcgcc gtaagccaac aattgggcga gtttcccggc aggtgcccgt ttcggtacga
     1141 aaatgacgca ggaaagaccg gctttggcgg cgttgcctgc caaagacgac gcagcgtttc
     1201 cggtcgacga acaggcgatt gtttgcgcat ttgtctcctt cgctttgact acggcaatga
     1261 tcgaggcgcg atccttgaga ctttgagtcg gattaaggcc ttcgtccttg atataaagat
     1321 tttccgtttc aagcaatctt cccaggttga cggccttgta taaaggcgtc atgcccactt
     1381 tcaaagtcgc tattgtgtct gtttccctaa cgggaagata ggcgagatag cgccaaatcg
     1441 actggttatt gtccgctttt aaggattccc gagaaatctg ttttcctatc gattcataat
     1501 cgtatgtgac gtccagaatg cctttttctc cgcaatgcgg gcaggtcagg cctgtgaacg
     1561 tctgcgggta tttacgattg caaatagtgc aaacgaatcc gctgatgttt tctaggctaa
     1621 tcttccatca cctccaaagg gatgattttg aatttgttga catcgaacag ccctttgtcc
     1681 gtcaccttga gttccggaat caccggcagg gacaaaaacg caagcgccag aaacggatcg
     1741 tcgatattgg gatcgacgcc catcttcctt gcttgtttgg acatcctttc cagtttctgc
     1801 tcgacatagt gataatcttt ttgagtcatc aaacctccgg tctccaattg caaataatca
     1861 aagatttcat taccatatac gagggcgatg cccccctgga tctccctaag tttgagtgcg
     1921 gcttttctca ttgcctcgtc gtcatcaccg atcacgacca tgttgtggga gtcgtgggcg
     1981 attgtcatgg caagggcgcc gcctttgagg ccatagcccc tgacaagagc gagtccgatg
     2041 tttcccgtca tcctatgtct ttcgactata gccagtttca agatgccact gtttttctcc
     2101 tgacggtaat atccgttttc caccctgaca ttttctgtta gcttcctagt tgtgagattg
     2161 tccttgtcaa agccgatcac atggactctg tttttatgga gtggtatgga aaaattgagc
     2221 ctttcgggat cgaaccgaac ggttttttca accgctgggt catcagtcgg aatgacgctg
     2281 aacagagctt tgccgtccct cgcaaccagt tggccttttt tataaacaag ccgcggaacg
     2341 atttttccgg gatcgtcgaa aacgatgaga tcggcataat atccgggggc gatgccgccg
     2401 gtgtttttga gattgtaaca ggtggcggtg ttgatcgtcg ccattttgat cgcgtctacc
     2461 atatccaatc cgttctcgat tgccatggtg acgttatggt ttatatgtcc ttccttgcgg
     2521 atgtcctccg ggtgtttgtc gtccgtacag aacatcagtc gggacagatt gtctttgtta
     2581 acggccttaa gcaaggtttc ttcgtttttg gctaccgatc cctcgcgcaa atggacatac
     2641 attcctcgcg acaccttttc tctcagttcc atagctgtcg ttgcttcatg atcggtttta
     2701 accccggcaa gaacataagc atttagttct tcgccggtaa cgccgggcgc gtgaccatcg
     2761 atcattttgt cggccatcac gtagagcttc cggtaagttt ccctgtcgcc cccgatgaca
     2821 ccggggtaat tcatcatctc gcccaaaccg acaatcgact tctcgttttt gagcgctccg
     2881 atttcatcgg cgttgattga cgctcccgcg gtttcgaaaa cggtggtcgg aacgcatgaa
     2941 ggaatcatta ttctgacatc aagcggcaca ttggccgatg attccaacat gaatctaatg
     3001 cccgagagac cgcaaacatt ggcgatttca tgcggatcgg cgatgacggt ggtcgtgccg
     3061 agaggaacta tcactttggc gaactggccg ggtgaaagca ttgatgactc gatatgtacg
     3121 tgtccgtcta tgagtcccgg ggaaacatag gaaaagcaca tgtcggtttc cgtgatgcct
     3181 tcgtatttgc cgatgccggc gattatgcct tcttcgatgg cgatatcgcc ggtttcgatc
     3241 aaaccggaaa aaacattcac gattttgcag tttttgagta cgatggcggc cttgtcgatg
     3301 ccaagcgcca cccggcgtaa cttttccttg tccatgccct acctcttttc tatnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcatcc agcagataca gaccgacaag
     3481 cgaatcgccg gccggggatg cattgccgat gtttcccgac aaagttccaa ggtttctgat
     3541 gcccggagat gccatttcac tgattatttc tttcaagata tcgggaatga tactggactg
     3601 gcggattgtt tccaatgtca cttccgcgcc gatgatgatt tcgtcttttt ccactttcat
     3661 ctgtttcagc tcttcgagat tggcgagata cattaccggg cgtttaaaat ccggaacgat
     3721 gccggattcg ctatagttct ggaccatgag gtccgtgccg ccggacacaa ggatgtaatc
     3781 ttcttgttgc atcaattcca aagcttcttt tagtgtttcg gaaatcacct gtttcaccat
     3841 agtccgtcac cgtcctcaga agctttcttg acggcatcca cgatcatgtt gtagccggta
     3901 caccggcaaa gattgccgga aagagcttcc ctgatttcgg attcggaagg atgtgggttc
     3961 tgtcttaaca agctttcgga ggcgatgatc attccgggag tacaaaaacc gcactggacg
     4021 gcgccgcact gaacgaaagc ctgttccagt attttgtaac gtttggtttc ccggaatcct
     4081 tcgattgtcg tcacctcatg tccggccaca ttggccatcg gaaccataca agaattgacg
     4141 acttttccgt caagaagaac ggaacaagcg ccacattcgc cttcaccgca tccttctttc
     4201 gttccgctca tcgcaagtgt gttgcgaaga taatcgagta agcggagcga tggatcggtt
     4261 tcggatacaa tcttttggtg attcagaatg aattcaattt cggccattgt cgatcaactc
     4321 cctgattttt tccggagtga cgggtatctc ggtcacattc ctcttgattg ccatctcgat
     4381 cgctttggcg atggccggcg ctccgccgac cagtgtcaat tcgccggcgc ctttggcgcc
     4441 atacgggccc aaagcaaacg gattatcgat aacgccggac attgtcggtg gcatatccac
     4501 cgcggtcggt atgatgtaat cggtcagatt tttctgtctc agtctaccgt ctttttgttc
     4561 catgacttcc agatatccat aggccactcc ctgggcaatt cctccgtcac actggccttt
     4621 gatgaccatt tcgtcgatcg cgtgcccgag atcgtattcg ctccagatgc ctataggctt
     4681 cacctggcaa gtcgccttgt ctatttccac ttcgacgatg ttgacgcccc aggaataggc
     4741 cggataagcg tcgcctgcca gtttttcttc gtcccatttg acatattccg gttgttggaa
     4801 gttttcttcc aagatctgtt tttcattcgc aagccatttt tctttcagtt ttcttgcgca
     4861 tctgtccacc agtccgccga cgatcatcat cgtccgagag gcgaccgtcg gaccagaatc
     4921 gggaacgaaa tcagtatccg gaagatcgaa aattactttt tccatcggta tttccagtat
     4981 gcttgccacg agctttcgca tcgttgtcct gatgccctga cccatgtcaa cggcagcgat
     5041 cagtattctt acgttgtcgt tctcgtcttt ttccaaacga acccgggctt tgatatgggt
     5101 ggcttcgccg ctgccggtga agccgcagcc gtgcaggaag aaactcattc cgatcccctg
     5161 atatccgttg ccggcagaat atatctgtgc ctttcgaaga taatcgcttt ccttcattgc
     5221 cttcccaatc agttccggca tgatgatcgg gtcgcggaat gttccgcttg tcgaggtttt
     5281 gtctccttgt ttcgccagat atttcatccg gaattcaaac ggatccaatc cgagaaattt
     5341 cgccacatgc ccgatgaaca tctcgataga aaaaagcatc tggggggcgc cgaagcccct
     5401 gaaggctccg tttggaaccg tattggtccg gtagacgcgt ccggtgacat ccatgtttgg
     5461 aaatgtgtag gcgccggacg aggcgatcat cgccctttgc aggacaacgc ctgacaatcc
     5521 ggcgttcgcg ccgccgtcca agccaacggc cgctctgatg gccgtaatct catttttctt
     5581 gttaatggcg gcttccagcc tgattctcga aggatgcctc ttggtcgtca ccggcatgtc
     5641 ttctttccgg tcgtaaatga gcctaaccgg ttttttcatc ttgtaaacgg caaccgccaa
     5701 ttggcaggcc attagagagg gaaattcctc ttttccgcca aacgctccgc cgactgtctg
     5761 ctggatgacc ctgaccatat cgtctttgca tccgagacag tttatcaaag cgtttttaat
     5821 ataataaggg cactgcatcg aaccggttat gacaatcctg tttccctctg gatagccaat
     5881 cagtccctga gtttccaaat aagcctgttc ctggtacccg gttatgtaga catcggaaaa
     5941 agtcttggaa gcggtttgga acggttgatc gccgtcccct ttggaaaagt gatagctgat
     6001 catgggatcg tcccattcca aattgggttt ttcttctttg taagcgattc tgatttcgtt
     6061 ggcgattttt cttagcacgg ctttgtccgg cccaaccagc aacatgatcg gctcgccgaa
     6121 ataagtgact tctgattcgg tgaaaaccgg ttggtcgtca aatatgattt tcacgaagtt
     6181 ttttccggga atgtctttga cgcctacggt agcgtatcct tctggtaaag gcggcaccga
     6241 tatgtcagtt attttgcctt ttgcgatggt agcgcggatt gtcttggcgt aaatttcgcc
     6301 ttccattttc agatcatcca catatttggc gatgccggat attttttctc tggcatcgac
     6361 tctttcaatc gttttgttta tgtcactcac cgttcattcc cccgtttttt ataaatgtga
     6421 taaccccggc gggacaaatg gcggcgcaaa gtccgcattt ggaacacttc gtatgatcga
     6481 acaggatatt tccttccaga taactgatgg caaaatacgg acacgctttt tcgcatatcc
     6541 ggcagcggtt gcatccgtct ttctgccagg ccgggaagtt tgccgtgaaa ctgacggtat
     6601 tcgtaagttg tgtatcaacc acttcctgta ttgaagaaaa tccgtgtttt tccaaagctg
     6661 ccggcaaagc ggcgatgatt tgacggtaca gctgttttcc tttgatcagg gcggccgaaa
     6721 gcatctggac cgccgaagcg ccggctagaa gaaattccag tacgtcgtcg gccttggcaa
     6781 tgccgccgac accgatgacg gtcaaactcg gttcagcctg tttgatccga taaacggtcg
     6841 cgagcgccac cggtttgatc gccggcccgg aaagccaggc gtaaccggaa gcattgccgt
     6901 atgccgtgct tctcttgtcg gtatcgactt tcatcgatgg gccaagggaa ttgatggcca
     6961 cgacaccgct agcgccggct tctctgattg ttttggcaaa tccttcggga tcgggcagat
     7021 gcggacttat tttcatatat accggttttt cggtgtgttt tttgatcgtt tccacgatat
     7081 ggcgtatctt gtcaagatcg gtaccgacat aatgggtgga tacttcgaag gcgtcggcga
     7141 atctgtccag ttcgggaatc agagtctcca tatcctcttc cgaataaccg acactcacaa
     7201 ttagcgggcg gtctttgcct ttcacgatgg cgggaaggaa ttcacgcgtc catttttcta
     7261 acggatattc gctccagagc tccgaattga gcacatggtc gtatcctccg tagatgcagg
     7321 gcttcggaat catcggctct tttgtcgata tggttttcgt agtcagcgct cccacgcctt
     7381 cacttgcgaa aaatgcgatc tttttgtcgt ctccgttcaa tggtcctgat gcgaccatca
     7441 aaggattctc tagttccagt ccgtcaaaag tagtttttag atccacggtt cgtcctcctt
     7501 gttcacatta ttccataaat cggaagcggc cttttttatg atggcttccg ttttcttgct
     7561 tcggttgaca agttcatgat cctttacaag catcttgccg gcaacataca catctttggg
     7621 agaaaaaccc ggatagacgc cgaagagcaa atgtcccatg gcgtttccgg aatcgatcgg
     7681 cgttgccggt ctgtagggaa caattataaa atcggcagcg aagccattcg caaatttacc
     7741 cagcgaaatg cccagttttc tgctcagata atcatatgtt tcgttgatag catctttgac
     7801 gaaatcggaa gacaggccgg tcaatgatcc gtatttatga tgcatggcat aatacaagtt
     7861 cagatattca agagccattc cggcatgtaa tccatcgttg ccgaggatta ctttaacccc
     7921 ttttgctttc atgttcttaa tgtcgggaag cccgacgccg ttattcatgt tcgaagaaac
     7981 attgagacag acgactgcgt ctttttttgc cagaagcgaa agctccgaat cattcagaaa
     8041 aaccccgtga acgatgaggg aatctttgcc gataaggcca tagttatcga atctgtcgat
     8101 tatcgacatt ccgtattttt caagacagtc ttccgtatcg tatttgcttt cagcaacatg
     8161 cacatgaatc gtttcgccgt cgaggttttc tttgacaagg cggatggttt ctttagacat
     8221 actcatcgag gcatgcatgc cgaaagtccc ggcggcccgt tctgttctgt tgtttttggc
     8281 aaaatcgcgg ttttcggcca ctgctttggc aacatcgaat cggtcgcttg tctcgaaggc
     8341 gaaaactccg cgcattccta tatcgttcac gaccgctttc ttgatcgctt ccaaggaacc
     8401 ggttatttcc ttgcccgaag catgatggtc gcaaattgtc gtaatgccgt tttcaagcat
     8461 ttccgctccg aatgaaagcg ccgaggcata tgctattctg ttgtcgattc cccggtcaag
     8521 tctccaccaa agctgttcca ggatatcctt gaaattcttg atgtcgagct ttaaatccaa
     8581 tcctctggcg aaagccgaat acaaatgggt gtgcccgcag gccatgccgg gaaggacaag
     8641 catgccgccg gcgtcgatcg tttcatggtc accttgcggg agtttctcca tcgggccggc
     8701 ttcgatgatc tgctcgtcaa acagaagaaa gccgtccgga atgaaacggt cgtagtcgta
     8761 aagcatggcg ttaatcagca gtttcgccat ttacagtctt ccgcctttca aaagccttcc
     8821 gaaaccgttc tcaaactcac ggtcgcggac aataaaggat ccgttggaca acgtggatac
     8881 aaccgttccg gctttttgga cacccatata gacggaataa tcgcttccgg aatgatcttc
     8941 ggcgattttt cccttgccgg ggcgatacag gaaaagatcg gcgtcgtaac cggccttgat
     9001 tttccccttt cgacccgcaa gtccgttcat ctgggctgga tgggtggtca ttttctccac
     9061 gatcttgttg ccatataagt tgtacatcac atcgaatgaa tgttcgatcg aaccgatgcc
     9121 aaggggaatc tccgacaaca gttcgccatg tttctggctt ttggtgaagg cgcaatggtc
     9181 ggttccgatc gcatagatcc agtcgatgtt cgcgttcaga tacatttttt cggttttgct
     9241 gcgaatcggg ggagcggtag tgtaaagata tccgtctttc tgagaaaata cgctgtcgtc
     9301 aaacaaaaaa tactgaggac agctttccaa aaagaaacgc cggttcagaa tctccggata
     9361 gtattccttg agattctcga tcgtttctcc ggaggaaacg tggaccatgt acaaaaatcc
     9421 tccgtagcgg gtgacatatc cagccagttg caaagccttc tgaatctccg cctgtttagg
     9481 acggcttatg ccaagttcac ggaatgtgta tgaaggattc agatcgatca gatcgtcgtc
     9541 ctcgacatgg accatgagca ggaaatggta tttttccgac agtttcaaaa gctcgatgat
     9601 ctcgttgtcg tttatcatcc gctgggaatc ggaataggta gtgaaacatt tgagcgtgtt
     9661 catcccgagc gacatcatct tgatgacaaa actctcgaga tcgcaatgtg gttccttgat
     9721 acaggcgtgg aacatatagt cgaccttgct gtcctcggct tcgaaaaccc gacggcggta
     9781 tttcatttcg agatcggccg gagtatcggc cgggtcgaga aaatcaatga tcgtcgtcac
     9841 gccgccaaga gcggccaaca gactgcctct ttcaaaattg tccaccgatc tcatgacccc
     9901 caggtcgaga tcaaaatgga catgcggatc gatcaagccg ggaaaaacca acattccctt
     9961 ggcatccact tccgtcgcgg ccggatatgt ctccgccgtc acggcctgga atatatcctt
    10021 gtccacataa acattggcgt cgacaaaatc accgtcctga tagactttgc cattcatgat
    10081 cgcaagatca accatcttta gcgcctccaa tcaaactatg cgatattctc cgatatcgta
    10141 tctctcaatc actttctcca aaagaccggt acgcaccgtt atacaggtgt ccagtccgga
    10201 cgaaatcatt gcttttagtg tattgtcgaa cacttttccc tcgatctcta acggaccgat
    10261 ctcatcaaga tagagcggcg aaacgttgtt tttgatcatt tcgccgatct tttcgccgat
    10321 catgtcgatc gcgtcctggc tgaaaaagta aggtccgatc cggcaagcct gccggaactt
    10381 gacagaattt ctgccttttc tcgaaacaag gggaaatttt ttcccggtcg acaattgcat
    10441 cgcgtcataa ccgaggacgg tattgccgtc catgtttttc agggaaacga atccgtcgcc
    10501 cttatgcgtt ttttcgtaaa tcgaaatcat tcttccggtt tttccggaat tgattttacc
    10561 agtaacgata cttaccatgc cttgtctccc gcatgtgcat ccccgatggt ctttgattgc
    10621 tgacggcaag catctccgaa gcgatggaca cagcgatctc ctccggagtg ttgccgccaa
    10681 gatcgagacc gatcggagcg tagaaatcgg aaagatccag atcgttcccg aaagctttgt
    10741 aggtattttc cagataatcg tcgagtttcg acggactgca aagcattccg aaatatttga
    10801 gttggatctt cttttccaaa atccggttca aaacgtggta atcatattcg tgactcggag
    10861 tcgatacaac gaccatcgag ttttccctga tccccacttc gtcgatgaac ggtacaaact
    10921 gattcgcatg cttttcgctg gcataaggat aatcgtcgag aacatcctgt ctgtcatcga
    10981 tcacgatcag gtaaaaaccc aaagtcctga gtacgtatcc gagggctttg gcgacatggc
    11041 cggctccgaa aatatagacg aattctttcg gtccgacata ctcataaaac aaagtcaccc
    11101 ttccgccgca ggccatcggg agcgtcttgg tttccggaat gatctttccc tctgatagca
    11161 gataggtttc cagcaggttc tcgtgttttt gcatcagttc gacgcatttt ttcttggcat
    11221 agaactcgat ggctccgcca ccgaccgtgc cgaaggccgt gccgtcttcc gctacgacca
    11281 tctttttccc gacatcgacc gggccggctc cttctttggc gacggccacg accaccatca
    11341 ggggtatccc aattcgtttc agctcgagca gggattcata gaaatctgcc atgtcaaata
    11401 atggtgaaaa caacggtaag cgccaatccg agggcaaaag tggatgtcga aagccaatag
    11461 gcgacatggg ttgtcggttt catttttgga aaaacaagat atgtgaacag gaagacaagc
    11521 ccggcagcca gaattcccga caacaggaaa ttgttttgca tgtaggaaag gaaattggca
    11581 accgaggaaa tattgtagtt aagataagcc gtgtccggcg acaccgccag attgacaata
    11641 taatagatcg catgaaaatt cagaaacaaa agattccatc ccagagcggc cagaggcatg
    11701 gcgcatatct tggaaaatag gttgctcttt tccaataatg gaatgacggc cacgaccatc
    11761 aatgtttcga acacgatggc gatcatcgga ttcatcactt tgaaataact gagattcgga
    11821 aggaaaaaat cgattccctt tatcagggcc gcgacgaatc cgatccagac aaggtcggat
    11881 ttcttctggc tgctcttata ggcccacaac aatatgacgg aagcgatcgg gaacatgacg
    11941 ctaccggcga tcagagccgg taagagatgc aaaacatatc ctagcgaggc ttcgagaatg
    12001 ccccaaacgg aaccgaacat caagatgacg ctaagtcttt gccaaaaatt ctttttcatt
    12061 tttcagtctc cttttttttc atggcgtaat atattttttc tgccgtcatg ggcagatctt
    12121 tcatccttac tcccaaagcg ttgtaaatag cgttcgacag ggcggccggt ggagtatcga
    12181 ttccgatctc gccgaccgat ttggccccaa acggtccggt ttcctcataa ctttcggcga
    12241 attcggtcgt cagtttaccg atttcttttc tggtcggaat ccggtaattc atgaaatcgc
    12301 tcgacaccaa ttttcctttg gaagtgaccg tcacattttc atagatcgcc atcccgatcc
    12361 cctggacgat cgcaccttcc acctggcctt tggccagatt cggattgatc ggagtgccgc
    12421 agtcgacaac gctgacataa tccaagattt tgatttcgcc ggtcttcttg tcggcttcga
    12481 tgtcgacgaa tcctgccatg aagggtggcg gcgatttgtg accgacatag cttgatgtcg
    12541 cctgtagttg tctttgtctt tctccgtaag tgagtttgtt ggaaagcgtc actaaatcga
    12601 ttgtttttcc gtttttgatg ttggtgaatg tcttcccgtc aaattcgatt gtatccaagc
    12661 ctccgtcgag aaactcgccg gcggcttcga tcatcatttc tttcagcttg cgagcggctt
    12721 tccagacggc gtttccggaa acgtaggtgg tgctggatgc gtaggcaccg gtatcgaacg
    12781 gcgtcaggtc ggtatcggac gaatagatga ctactttgtc gatatccgtc atcagttctt
    12841 cggcggcgat ctgcgacaac acggtatcgc ttccctggcc gatctcggtc gctccggtca
    12901 tcaggttgta aaatccgccg tcgtttaatt taatgctggc ggatcccatg tcaatcagag
    12961 gaattccgct tccctgcatg gcgattgcca ttccgaccga acgcaccttg tcttcggttt
    13021 cctcgtaacg gggatactta ttatcccagc caatgagttt ttttccctgt tttacgcagt
    13081 agtccagttt gcaggaatcc atcgtcatcg cggttccttc agtgccttcg cccatgatgg
    13141 cgaagatcgg cgatgtctcg ccttctttta tcatgttctt ctgtcttaga acaatcggat
    13201 ccattccgag tttatccgcc aggatgtcga cggcagcctc gagggcgtaa ttgccttgaa
    13261 ttgccccgta tccccgataa gctccagcgg gagtatggtt ggtgtatacg acttttccgc
    13321 cgaatctgac ggcagaaacc ttgttgtaaa gcggtaagac cttggatccc gtcaccatga
    13381 atacggtcaa agcatgttcg ccgtaagcgc cggtatccga caaaacctta caatcgattg
    13441 ctttcatcga gccgtcattc atggcgccta tcgcaatatc tattttcatc ggatgacgcg
    13501 tataggtaga ttcgaatact tcctttctcg tgaagacgat ccgggaaggt tttccggttt
    13561 tccaggtcac ataacttgcg aacatttccc cgtggagagc ctgttttcct ccgaagccgc
    13621 cgccgacacg cggttttatc acacggattt tggcaagcgg aatgcccaga gtctgggaaa
    13681 taatccttct tacatggaat ggtgtctgag tcgatgaata aatgaccaga cggttctggt
    13741 aatccatccg ggcattgacg gcatgcggtt ccatcatcgc atgggcctgg gcctgggtca
    13801 ggaaggaatc cctgatgact acatcgcatt tttccagttc ttcctcgacg ttgccgactt
    13861 ccatttcata actggcggcg atgtttcttt tcggttcgaa accgatcggg aacatctcgt
    13921 ggatctccgg ttccggatga atgacggatt gattgtcgat cgctttctcg aaatcgagga
    13981 cggaatccag gacttcgtat tcgacatcga tcagtttcaa cgcttctatg caggcttttt
    14041 ccgaggtcgc cgcaacggcg gccacttcat cgccgatata acgcacgtat tcgtcaagaa
    14101 caaacttgtc atgcggcgat ggttccgggt aaccctgtcc cgccctggta aaaggtatgc
    14161 gagggaagtc cttgtaggtc aaaaccattg tcacgccggg aacgtctttg gctttttccg
    14221 tcgcgatttt cgttattttg gcatatggat gcggacttct caggatcttg atgacaagcg
    14281 aatctttttc cgcgaaatca tcagtgtatc cgtggcgtcc catcatcagt ccctttccgt
    14341 cgacggaagg gacgggattg ttcacgactt tcatgatttg tcacccagat atttctcgat
    14401 tgcgatttcc tgggcctggt atcccgtgca acggcacagg tttcctacaa gataattttt
    14461 gatctcggac ggcgtcgggt tcttgagttc gcgtttcatg gcatagaccg tgagtgccag
    14521 tgacggattg caataaccgc actgatcagc tccctcatga ccgaaaaatt ccgccaattt
    14581 ttccgcctct tccataatcc cttcgactgt cgtcacttca tggttattga cagcgacgga
    14641 aagcaaggaa caggaaggca ccggtttacc atccaaaagg attgtacaga cgccgcagtt
    14701 ggtggtgtcg cagccgcgtc tcacggaaag ataattgttt cttctcaagg tgtcgaccag
    14761 atattcattc agatcgacat cgaaatgttt ttcttccttg ttgacaacga ttctaatccg
    14821 catttgtgca ggtcgcctcc aatccgcgtt cgacatatgt tcttgccagc atttcccgat
    14881 attccccgct ggctttgttg ttggataaaa attccagttc cctgaccgcc atctgggcca
    14941 ccgccgctag attttccttg gtatggtctt gatctttctg cagtgcttcc gctgccagcg
    15001 gacacagtct tgcaataccc ggtctcgctc caacggcgat acgccagatg tttccggttt
    15061 tgacgatcgc cagattgaca atggcgaagt cggtcgacgt tctggcaacc cggtggaaat
    15121 aaccataacc cggacttttg tcgatctgga catacataag cagatccttt tccggttttt
    15181 cctgattcag atattcagaa attttcatgt ttttttggct atagaacaca agcgtggcgt
    15241 caagcgccaa tagtgcggtg atcacatcgg aaaatgggta tctgcccatg attgtgccgc
    15301 cgattgtggc gatgtttctc aaagtcacgc ccataactga tgccatagcc attgagagaa
    15361 ttccgccaga tatgttcttg atggcggtgt ccgtctcaat cccgcggagc gtggcatttg
    15421 ccccgattat gactttgtct tcggtattta caatcttgtc gagacccaat ccttcaaggg
    15481 atatcagggt attgatcctg gaaccggtaa gtttcagcca agcgccgccg cccaaaatct
    15541 ggtttttggg cgattccatg tatttggcaa aggcttcgtc cagggtggtc ggtcggaaaa
    15601 atgattcgat aatcatgtta acgctcctcc tcgtgaaggt tttgtatata gaaaaaatct
    15661 ttcaaagtat ccacgtcttt gatgatgcct tcgtcgtcgg tttcgaagat ttcgacatcg
    15721 taatggttac gaaagatttt taggttcgaa tcatctggtt cgtcaagcaa agcttccttc
    15781 agccgccgag agaaaaaaac cggatggccc ttacggccgg cataggcagg cacacgtaca
    15841 tccttggtac cggaaagcaa cagcttgaat gtggcttcct tcaccaaggg gtggtcgccc
    15901 ggaagaacga aaaaatcgga ttctgtctgt ctcactccgc atttaacgga agaaaacata
    15961 cctttttcat agtcggtgtt gcggatgatg acgacattgt cccatccgga aaaaagtttg
    16021 gaaatgtcgt catggtaatg gccggtgacg attatcaccc ggcttgtgac acgagaaacc
    16081 ccggccaccg tatgccaaat cagtggatgg ccggccagtt cgagcatcat tttgttttct
    16141 ccgatacggg atgaatatcc gccgcacagg attacggctt ccgccacagt gtcaccaccc
    16201 gtgaaagata ccgaatttta ttcggttaga accggtttta tattaaaaga tattatatca
    16261 tataattggt aatttgaata gtgcctattc agaagagtaa agaaaaaaat gacgggaata
    16321 tcccgtcatt ggttctttga agccttcact acgatgtttt tcggatcatc tacaacgatc
    16381 aagtgtttct tttcgatcga cttggtgatt aaatcggtga tgtcgtcgcc gtaacttctc
    16441 agggtcttaa gccctttgta catcgcaaat tccccgccgc cggcatatcg gtaattattc
    16501 atggctaagg taagtatgtc gttttccctg acgggaacgc cgtttctttc tagcctgacg
    16561 attcgactgc cttccggttt tgatatgtcg atagtataat cgatgccgtc gaacatatcg
    16621 taattgtaat gttccacctt gggatgggaa tacttcggat tgcttgatat gcggccattt
    16681 tcaacgacaa agtactcggc gcatttttcc aaagccaacc ttaatttctc gccggtgatt
    16741 tcgacgacta ccagattgtt cggatacaaa taggtcgtat agacatccct gacggtgatc
    16801 atctttttaa atccggtgac cgaattgcca agggaagaac aggtaatcat ggctttgctt
    16861 ttgtctagct gcacccggtt gatgaaagtc acaaccttgt gtttgtggag cctggcgtcg
    16921 aattgatcct tgatttccat atcgttctta ggagctatgc cgatgacttt gtccaagtac
    16981 tcttccgttg ctatttgttc cttttcgagt cgtttgcata tctcaacgtt ttccggataa
    17041 ccggtagatt ccataatttc ggcatcaata ccggtaattc cccatttatc gccgtttttc
    17101 gcaaatttag ttataacctt cccgaccgcc aaggcattcg acattggctg gatgacccag
    17161 gtgtttttga ccttttggca aatcttcctg tgctggtgtc cggtcagtag cacatcgatt
    17221 ccttcgattg tttccagcat tcgcgatcct tcgttttctc cggtatcgtc gacatatggc
    17281 aaaaaggtat ctagatcttt ttcgaatcct ccatggtaag ccactatcac catcgtcgga
    17341 tcttcttctt ttctgattct ttcgaccagt ttcttggctt gcaggcaagc gtcagtgaag
    17401 acaagccccc gcacattggc ttttttttcc caattgggaa tgtactgggt cgtaacggca
    17461 atgatggcta ttttcaatcc ttcggtcaat tcgacgatgt cgtatggcga cccgatggcc
    17521 ggattccctt ttttcgtcag gatattggca cataggggtt tggccgtgat ttggctgatg
    17581 aaacgggaca ggtaggctcg cccgtaatta aaatcatggt tgcccgggac aaaataatcg
    17641 tagccgatcc ctttcaggca gaaagctgcc ggattggctt tgtttctggc tttgtgccag
    17701 tggtaatcca tcatcggact gccttgcagg atatctccca aatcaagtac gacagtgttg
    17761 gcgatccggt gttttcggat cactgacgcg atattggcaa gcgaattcga ggcctttttt
    17821 tccgtcacgt aatcgatcgg atatagatgg ccgtggacat cgctcgtaaa taacaagcaa
    17881 aattccattt cgtcattcaa tctgtctcag tcctttccgg aaacgttctt tacggattat
    17941 tatcgcatat tgcgtttgat ttggcaaaaa aacaggtatt tcaaggcttt ctctggcggg
    18001 aaagccagga aaacatggtt gtctttttgc cgtcgatgac atcaacgcac cagtcgttca
    18061 aagccgtgat ccaggagaaa tgaccgtggt aacgatatgg agcgccatta tgaagcgtat
    18121 atttgccagt ttggtcggtg acgtgctcga tcaaggtcaa atggatatcg ttggattttg
    18181 tttccaaaag ccttttgcag gtgggaaggc aaaaatcctc cggcctgacg acggggtcgt
    18241 cacgagactg gacgaaccaa agcggaatgg tcgaaagcac tctgatatcc tcgtctttca
    18301 gccatctgtc gttataaacc tcacagacgg ggaaccccgc agcaaagagt tcgggatatg
    18361 acaaaagcat tttgatcgtc atgaagccgc cgttcgaaca gccgccgaca tagacgcgtt
    18421 tgggatcgag acggggatgg gagcgcagga tgccggtgat caggccgtga agtgcctttt
    18481 tgtacattgt cgtcccgtca accgtataat tgcctttgcc atcatccatc caaaatgttt
    18541 ccgcctgggg gatcaatatg gcaaccccgc cgaagatatt ctggatatgt ggttcaacca
    18601 acctggaaac cggtggtttg agaagcctcc gccagacgct tcttccgcct tctcccgcac
    18661 cgtgcagcca aactataagc ggaactggtc cgactgattt cggcatataa aacgaataat
    18721 ccatggcgat cttccggtca aattcgatga cgccatccga aaacatggca attgaaggcg
    18781 ccatgatttt cttttgtata ttcacttcga tcaaagatat ctcttgatta tcataagtaa
    18841 aagactcttt aagccttatc ttgaagacga tttccttttt cagttcgttt ttttcccttg
    18901 gttcgagaaa atccatgggc gacagaaaat tttgatctgg gtaagaatcg aaagtgacca
    18961 ccgtgccatc gacgcaacga tacgttgaaa agctactgag ggttccgctt tccgatacgt
    19021 tgtttacgtc gattctttcg attggcggca tatcggtaaa cttatcgatg aaaaacgccg
    19081 taatggccgg tccaaagtcc atgctttcca ccaggatatt tccggatagt ttcaagatac
    19141 ccgccatatt cttcccccct cttcatagag aattatataa tcaattgcct acttcttcaa
    19201 caaatccctt aaaaagaatg tcgggtttcc tgtttccaga taaccttgcc atacttgaca
    19261 agtgtggctt ttagccataa acttatggca gaagacaacg aggtgataac atgtgcgaca
    19321 ctttatatag aaaaaccgga acatcggcgt ttttcgccaa gaacagcgac cgcagtccca
    19381 acgaacccaa tctgacagta tttgtcgaag cgaaaccggg agatcccttg aaccggaaaa
    19441 aatgcactta tgtggaaatt gacgaaaatc cggacacctt cgcgatgatt ctggtgaaac
    19501 cgagttggat gtggggcgcc gaaatgggaa tcaactcgca aggcgtcgcc atcggtaacg
    19561 aggcggtatt cacgaaaaac cgggacaaaa aaacggaaag actcaccggg atggatcttc
    19621 ttcgtctcgg tttggaagaa ggcgacaccg ccaggcatgc tctcgatgta atcatcgggt
    19681 atctggaaaa gtacggtcag ggcgggaatt gcgggtttga caagccgttt tactatgaca
    19741 actctttcct gatcgccgat tcccgggaag ctttcattct ggaaatcgtg ggaaaaaagc
    19801 atatcgaatg gcaacttgac gccagcggca acatatccaa ccgcctgtct gtgatgaatg
    19861 aaacagccgc tgcttttttt aaacggaaca ccgaaccggt attcacttgc ttttccggtt
    19921 ctttcaatcg ggaaaaaaca ttgagagaag ctcacatcac aggaatcaaa gatgtgataa
    19981 aggcgatgca aatgcatccg gctggtctga ctacaaaaac acttttccgg cgcggaaccc
    20041 tcaaaagtcc ctgcatgcac tattcaacgc ttggcgatca tacgacttcc agcatgattg
    20101 tcgaatggaa aggcgacaaa ataacgatct ggctaaccgg acgctcgacc ccttgcctgg
    20161 cgatatatct accagtcata ttcgatcggc ccggcattcc ggttttcaaa tcggaaaaag
    20221 aatccctcga tttctggctt gaacgcgaat atctgtcgcg tgccgttttt tcgggactag
    20281 tcatagaacg ggactataaa atacaaacaa acgccataga agaaaaattg atccaaatgg
    20341 cggctggact gggtgacgat ttgtcttttg aagagatcgg cgcattcaac aattatgtat
    20401 tggaaatgga agccggtttt gtcaaagaat atgcaaacat catccataag gtacaatccg
    20461 atccctccgt tctgactgga cgctggaaaa agaaaaccct gacgcttggt aaaaacgtct
    20521 ttttggcttc gctggaagaa agacggaata ccagatgaaa atcgtgctcg caccagacaa
    20581 atacaaggga gcgctgaaaa gcattcaggt cgccaatgcc atggaaaaag gcattcggaa
    20641 agtacttccc gatgccaaga tcgtcaaatt gaacgtttcc gacggcggcg agggcctggc
    20701 ggatgcgatt ctggcatgca gaaaaggccg gaaaatcgtt ttaagaacca ccggtcccga
    20761 aaacaaggaa gtcacaagtt cctatgcgtt tcttgaagac atgaccgccg tgattgaatt
    20821 ggcggagagc gccggattgg ttttggcaaa gaatccgaat cccgccaaaa ctacgacata
    20881 cggaacgggc gagatgattc ttgacgcttt ggaccgaaac gccaaggaaa tatggttggg
    20941 cctcggcggc agcgccacga acgatgccgg atgcggaatc gccagttgtc tcggagtgaa
    21001 atactttgac aaatttggcc gtcgtttcgt tccgacgggc gataccctcg tcaaaatcgc
    21061 cgcaatcgac acttcggaaa ttgacgaaag aatcaggaat attaaaataa tctgcttaac
    21121 agacgtggat aatccgcttt acggaccaaa tggcgccgcc agaatctatg cccggcaaaa
    21181 aggcgctgat gacgccatga tcgatctgtt agacgaaaac ctggaacatt atgctaaaat
    21241 cctgcaagac tcattcggat ttgacgccaa ttttgccaaa gcgggagccg ccggcggcac
    21301 tccggtttcg atgaaggcgt ttttccatgc ttctgtcaga aacggagttt tattcttact
    21361 tgaagaatgc ggcttcggcc aagtcatcaa agacgcggat ctgattttga ccggcgaagg
    21421 ccgtttcgat catcagagtc tacagggaaa agccgtcagc ggaatcatga gaaaagcatc
    21481 tgaagccggc gtttcggttt atgtcctggc aggatccgtc gagaaaaatc ttcttcttta
    21541 caagccgaag gaaatcgccg gtctgatcca ggtgactccg gatgacatga acatagggga
    21601 agcgataaaa aatagcgaaa ctctgatcga ggcctccgcc gaaaatctca tgatcaaact
    21661 tataaaaaaa tgaccgctcc gtagatttcg gggcggtctt tcaatcatct gtggtttttg
    21721 cggttgtaca ggcagtcgtc gatcatgacc acgagcgcta cataaaagga cgcattttcc
    21781 tcgtcggaca cgccgatctc gtaactgtct ccccatgaca tccacttctt gtggatctcc
    21841 accacggctt ggccggcttt ttcgatcgtg aaatcgtgag ccatgaaatc acccgtgatc
    21901 gaatagtcgc cgcgactgct taagatatcg atccgatggc tcaaaacggt aaaacgtttc
    21961 ttgatattcg ccacttcctt gccagatgaa tcggtaagac tgtaatgggg caacagatgg
    22021 aaaagcatcc ttttgatcgt gaacagaatc tccccggtcg gttcacagaa atcttttctt
    22081 ctggtgaagc ttatccatgc gcctttgaca tggaatactt cctttccgct ctcgtcgaac
    22141 acgctgtagg tgtccttcaa ggccaatacc ttttgcttca gatagtatct tttcatcttt
    22201 acctcccttt ggcggtcaaa ccgctttttt tcattatcat tatatcattt tccctatcat
    22261 tttcatataa ctgatataaa tttctttttc atgtttcatc acacataatc ttcattatat
    22321 tgtcataaac acaacatctt tttgcgttat cattacgggt gtaaacaaaa agcctgacat
    22381 cagggaggaa catccgtcat gaaaccgatc ttcttgaaaa aagccttgct cgcatttatg
    22441 atcttaccgc tattattcct atctggttgt gatcttttca caacaggcga tgcatcaact
    22501 gatacatccg acagcattat caatgtatac ggaatcgacg aaagcgttct ggaaagcctc
    22561 gccaccacga acgactatcc gtcgacagtc accgctgccg ccattgtgat gccatctacc
    22621 gtggagataa ccgccgaaat aagtttttcg tacacggcct catattatag cccctgggga
    22681 tcgagaaccg agacggtgac cgattccgcc atatcggaag ccacggcatt tttcatcaac
    22741 gaggacggct atttgcttac aaacgcccat gtggtgacgt tgtccgatta tgaagactat
    22801 ccggggttca cctacaccgg atggaatatc accctgaatt acgccgacag cgacacaata
    22861 ttctcggctt cgatcgtcga atacgatgaa gctttggatc ttgcgatcct gcaaatagac
    22921 gaaccgatcg ccgatcttgc atacgtcact ttcttcaaca tcacttctcc ggatgattcg
    22981 gcttacggaa cctccgaggc gatttccctg tattacggcg aaccgatcat tgccgtgggc
    23041 aacgcggaag gctacggaat ttccgtcact tcgggcgttg tctccgctcc gctcagatat
    23101 ttcgacgaca gcggcaccgt catccaagcc atccagaccg acgccgcaat caattccgga
    23161 aacagcggcg gtccgctttc caacatgtgg ggggcgacaa tcgggatcga ttctttcaaa
    23221 atcgtgactt cgacttcgga aaatctcggt tatgcgattc cttcctatgt ggtaatggat
    23281 tatatcgatt ccgtttccga ctcgatccag tactattaca cgaacgcccg ctccttcgac
    23341 aaaacggatg tcataagtca tctcggttga agcaggactt agatataaat cttcttctcc
    23401 tccttaaaaa acaggattct gccgaagtcc tgtttttttt atctcaagtt gtcttttcct
    23461 ttgttttcat ccggaagacg aagaatagca tcaaggcggc agccactccc atcacggctc
    23521 cgaataagaa ggtgtactcc atcccgattc cccacaaaag gccggcgatc aaggacgcga
    23581 aaagcagtcc gaggccctgg gatgtcccgt aaagcccgag gacggttcct ttgtattcag
    23641 gctgggatat ttcgacgata taggctcttt ccgctccgga aatcaacgac tggaagattc
    23701 cgtagaatcc gaacaaaaga tagaaaccga gtttgtctac gttcatcgcc actcccagat
    23761 aaacagcgga atagagcagg taagcgggaa tgatcagacg cgaccgtttg atcttgtcgg
    23821 acagttttcc gagcggatag gacgaaagcg acgacaccag gttgtagact aagtatatca
    23881 ggagcacgtc tatggtggaa tatccggcgg cgttcagtct taacaccaga aaggcattgc
    23941 tggaatttcc gatcgagaat aagaatacga cgactagata agcgaccaaa cgcttttccg
    24001 gcctgaaact tccgggtccg gattccttta tcgcttttcc gatcgttttc gtctccttca
    24061 caaacaaaag acaaaacaat ccgaaaagcg ccgggatcgc ggaaaaaagg aaaattttct
    24121 tgaacaccgc catgtcggcg gatgtttctc cgtttttgac aagcaagctc aaaagcaaaa
    24181 tcgaaacggc cactcctgcg gccgaaccga acatgtcgag cgccttgtga agtccgaaag
    24241 ctcttccgag ggtcgcctgt ccgctttccg ccacaagggc gtcccgcgga gcggtcctta
    24301 ttccttttcc gatccggtcg actaatttgg cgattagcac aaaaacccag ttgaaggaaa
    24361 agaacagcag gatcttgtag atgacggcgc tgaaataacc ggtgaaggcc aggatttttt
    24421 tcttgccgga cttgtccccg agataaccgg aataactttt gagaatcgcc gctacgcttt
    24481 ctgccaatcc ttcgacaacg ccgatgatcg ccacgctcgc gatggtggac aggtagatcg
    24541 tgacaagcgg atacaccatt tccgtcgaca aatcgatgaa catgctgaca agaccgagca
    24601 atacgatatt tttaaatccc tgtttcacgg acagggattt ttctttttcg ttaagcaatg
    24661 tattcatccc cacatatata tgacaattaa aaaatcagcc aaagcaaaac cgttccgaga
    24721 aaagccgaag tcagattggc gaaaaacacg gtgagaaaca gtttggtccc ccgggaagcc
    24781 agttgcgaaa gtaccatgta ttcgatcagg aaaaccgtga tttccaaact gacgaaaagc
    24841 acgatttctt ccttcccgac gttactcagg aaaagcgaaa gcggtccggc agtcaaattg
    24901 gaacggtaaa cgaaccaata gatgacggta ttcaccgcaa gattggtggc gatgtttatt
    24961 aacagaaatc cgagaacaaa caatttgtca cgatatttgc cgaaccaata aaatggaaat
    25021 tcgatcaaga gcgtcataac aaccgcagtg ataaaaacca gaaccagttc aagcatcttt
    25081 cttgacgtcc tcctcc
//
LOCUS       DAOPJL010000018        24775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_2119_length_24775_cov_7.316626, whole genome shotgun sequence.
ACCESSION   DAOPJL010000018 DAOPJL010000000
VERSION     DAOPJL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24775
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_24775_cov_7.316626"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..139)
                     /locus_tag="PLP02_03525"
     tRNA            complement(53..139)
                     /locus_tag="PLP02_03525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Leu,seq:gag)
     gene            complement(209..1420)
                     /locus_tag="PLP02_03530"
     CDS             complement(209..1420)
                     /locus_tag="PLP02_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPF42555.1"
                     /translation="MLKGNLLYGQSGGPTSVINASAYGVISEARKNPNIKDVIFMKHG
                     IKGALNEDFFFLKDQDEETVELLKHTPGSAFGSVRYKLKDFREDDSNYLRLLYIFQKY
                     NIRYFLYNGGNDSMDTCAKIADYMNHIDYDCYIIGIPKTIDNDLPHTDHTPGFGSAAK
                     YIINTMMEISYDMLAYPNGKVTIVEIMGRHAGWLTASSALAQLSGFGPDLIYLPEVPF
                     SVDKFLKDVKQVYNEKKQCLVAVSEGIMNEEGIFISSSSGLKDAFGHFQLGGVSSKLA
                     TTISADLGIPSRSVELGSTQRAASHIQSLTDVTEAIEVGKNAVRQAVAGVTGKMVTIN
                     RISSNPYEVEYGLFDLTECANTEKRVPLSMINSEGNGMNEELVEYIVPLINGENPPKY
                     KNGIQQFSRFK"
     gene            complement(1429..1746)
                     /locus_tag="PLP02_03535"
     CDS             complement(1429..1746)
                     /locus_tag="PLP02_03535"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HPF42556.1"
                     /translation="MLYEKPTQEEIEAVMQSFFPSKDPLRMPVMPTKRRKQFVAFYIV
                     SGCFTKGICYTEKEINAILEQINPDYATIRRSLIDYGLMERTPYGSSYWLKEEKAIET
                     KER"
     gene            complement(1753..3771)
                     /locus_tag="PLP02_03540"
     CDS             complement(1753..3771)
                     /locus_tag="PLP02_03540"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPF42557.1"
                     /translation="MPIKNVKLPIYLSRREKDGFEKNFLPLLRTSELVEVRMYSLEYD
                     WIMASKDSLLQFVSQGGKMRFIVSLCHLDNREKEAFMLHRDDEVESIIKKEIVDRFKM
                     DLPFYEQRFLQYLSYLVAAGILEIVVSVPKHDQYPLDQIIFLSEGKDTICFHGPFFGP
                     VDPLDATDIFVSWDQRDHLRIDSYKGEFESEWRNKADNVWCVPLPQSVRAEFASLAVS
                     SSPYEYLFEDRVKYRMRASQQEAIDLLVSSDWKGIFTLPDGCNQMMTCMFALDQYAVR
                     YPRAVALIVAPNLHAIAEWEKIVANKYPGKQRYFLTNEEDVREVELGEILRLKLKDDF
                     IFVFASYRVFASENFQAAFHKNKNYSFYIFDECHLLVESNDFKDCEYLDNSARIGLSS
                     TPMPWLSEDSKDKLEQIIGNELSSQPLKNLLGKSHNEYEYNVIMSELIVPEYSEFRQL
                     TGMALRAESPEKAALYRLQKERVVEKAFNKTVDFLRIFMTSVRKRVIIYVDHTQTDEL
                     KRTLEFQFGVNTEVFTPEVDLQSRLRIIKSFQNGALDIIITSDVFTEGIESLAPAAIY
                     MLSTPTIPRIWQQRRNISLYPQIHGKVKIYDFVTVAPVQEFQDAMHMEVVKRELPRIS
                     DLSRLASLCKMDEFLEYLEQNNLDNVFQNTEPSELNHDIFVKREVAME"
     gene            complement(4236..4309)
                     /locus_tag="PLP02_03545"
     tRNA            complement(4236..4309)
                     /locus_tag="PLP02_03545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4275..4277),aa:Gly,seq:ccc)
     gene            complement(4423..4782)
                     /locus_tag="PLP02_03550"
     CDS             complement(4423..4782)
                     /locus_tag="PLP02_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42558.1"
                     /translation="MNIKKMLPYLCKEDLNSLLEACVKGEITEEDFKVPYLYPFLEPE
                     QLMTVLRSAMEGTINVSPKGILPFLEEEQIDELLNELQETENDKLAVDDLLPFLEDKQ
                     VRKLFGDILEKSKNKTE"
     gene            complement(4787..5314)
                     /locus_tag="PLP02_03555"
     CDS             complement(4787..5314)
                     /locus_tag="PLP02_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42559.1"
                     /translation="MLDMVKIGNKLCELRQKKNMTQEDLANVLLVSHQAVSKWETGKA
                     LPSVDTLAEMTSFYNTSVEEILCLYESITPKGGEELFREHSRDWVIHETATGKIKGVG
                     LEKIIHLLSQTEREYALYLMTEEGIEIGDNLWPRLSYDERAFLINKYRDKKYPLQIDR
                     INSMMTDAEKRKLWR"
     gene            5704..6162
                     /locus_tag="PLP02_03560"
     CDS             5704..6162
                     /locus_tag="PLP02_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPF42560.1"
                     /translation="MSLKNILEKYPRNESSLIKILLEFQDTKKDNSISESEVKEISDY
                     LGVPESKICSVISFYSFFSFNPRGKYVILVCKDVPCFVNSQVSIKDALEESLGIKMGE
                     TTKDGLFTLEYTGCLGCCDNPPAIEISGKTYCGLTLKKVREIIDEYRRMA"
     gene            6162..7418
                     /gene="nuoF"
                     /locus_tag="PLP02_03565"
     CDS             6162..7418
                     /gene="nuoF"
                     /locus_tag="PLP02_03565"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPF42561.1"
                     /translation="MLEMKLVSKRCGVIDPLSIKDYIDNGGYKALAKALADGKEKTIS
                     EIQASKVRGRGGAGFPLAIKISSVAKEIADVKYLVCNADEGEPGNFKDRYLMENDPHG
                     MIEGMVIDAYLTGATKGYIYIRGEYQKAQKIVETALEAAKNANYLGKNICGAGFDFDL
                     EIRSGAGSYVCGEEFALLESIEGKPGRSRVKPPFPTQKGLFGKPTQINNVETFATIPV
                     IILMGGSEYQKIGTPTSTGTKLVSLCGNVRNKGVYEIPFGLSLREVIDKLGGGVDGGH
                     KIRMVQLGGASGPIIPESMIDLSIDFDHFDVFSAKAGSGALIVIDERADMFEILQRIM
                     EFFVHESCGKCTPCREGNLQLLKIINRFVEGKGRKSDLETLSILTNVMKNTSLCGLGQ
                     ASPTAIISTMRYFPDEYEKRLAKDEM"
     gene            7430..9151
                     /locus_tag="PLP02_03570"
     CDS             7430..9151
                     /locus_tag="PLP02_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPF42562.1"
                     /translation="MINLKINNIEVSVPEGTTILEASRANGINIPTLCNYPDLGIKAE
                     CRVCVVEIEGRRGLSTSCSTICEDGMVVKTNTPDVITARRTITQLLLADHDVDCPSCP
                     KNLNCELQELANRLNIVDNPFDHVEFPQPVDEDNPSIIRNPNRCIKCGRCIEVCREVQ
                     GIDVLEYMGRGVSEAITPAFGRKLNDLFCTYCGQCTSVCPVGAIVEKDDTDLVFQAIN
                     DPSKHVIVQTAPAIRVSLGEENGMNAGTVVTGKLVSALRALGFDEVFDTDFTADLTII
                     EEGNELIQRITTGGVLPMMTSCCPGWINFAEQEFPEILPNISSCKSPQQMFGALAKSY
                     YAEKIGVNPKDLFVVSIMPCTAKKYECKREEMQVNKGIQDVDVVLTTRELGKMIRQVG
                     IDFENLKESEFDAPFGITTGAAAIFGATGGVMEAAVRTVYEVVNKEELKSLDFKGLRG
                     LDGIKEATVTLGGQEVKVAVCHTLSKARILAEQVKNGTSPYAFIEVMACPGGCIGGGG
                     QPYGTVNKIRSKRIDATYNVDKDMPIRKSHENPAIKSIYENYLEKPLGHKSHHLLHTT
                     YTSRNKN"
     gene            9332..11032
                     /locus_tag="PLP02_03575"
     CDS             9332..11032
                     /locus_tag="PLP02_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPF42563.1"
                     /translation="MKRNIGFAFLLIIALLLSGCTLDGSDTAIITNQLNSDDTRVSFD
                     TFDDTITFYNTEITSTNGVVNISQNIVTISTAGVYLFSGSSLNARIVVNAGDEDNVFL
                     VLSGLDLTSSEDAPITITNADKVIINLVEGTINTLTDSASSLNDHSACISSSDDLSIN
                     GSGSLTINANYKNGIDSNDDLVIAGGNIAILADNDAIKANNSFTMTETTLTLESDNDG
                     IHVESDESSLGNVLLISGTLSVSSYGDGIDASNQVVISSGTYNLVSGVGNSNIDSVSG
                     KGIKATSLIMIKDGEFTINSKDDSIHSNDDLRITGGNFDIYSGDDGIHSDTSLEISGG
                     TFNIARAYEGIESLSLEISGGFIYINSSDDGINGADGTDSSGTFAWDPHAGGGMSSGN
                     ASLKITGGTIYINSIGDGVDINGSISMSSGALLVAGPTSSDNGAIDYDGSFTINGGLL
                     VCAGSSGMAQNVSSGSSQTTVLITLSSTTSRMFHLESADGENIATLSPGKSYQSILVS
                     SPKLNVGSSYTIYIGGDMTDFATEIYGYYDGGTYVAGTEYATFSISQTISTVGSSMYR
                     "
     gene            11038..11757
                     /locus_tag="PLP02_03580"
     CDS             11038..11757
                     /locus_tag="PLP02_03580"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPF42564.1"
                     /translation="MSQSVFTRYEEKYLLTEKQKNDMFGCLAHYLPFDSHSPNGASYN
                     VYSLYFDTSDNNVIRQSLEKPKFKEKLRIRCYEPPKNDSDPVFLEIKKKDHGIINKSR
                     IVIGYGKALEFVATKQIPAFLSDDYFTNRVANEIEYMLEQNTLTPSYFICYKRQAFAA
                     RETYFRVTFDHDILYRNTEPSLLDLEGQPVIPADKWLMEIKAENNYPLWLVRKLSELG
                     LANQSFSKFGRAYQNTLKGEF"
     gene            11759..12445
                     /locus_tag="PLP02_03585"
     CDS             11759..12445
                     /locus_tag="PLP02_03585"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPF42565.1"
                     /translation="MLLSIFVLDTETLTLGNVVLVMLFATILGAVLTGIYALTHRLLG
                     FDRSFCVTLLMLPIIVSIIIMLVSNNIARAFSLAGVFTLIRFRSTLPDTRDITYVFAS
                     VAIGLIVGMGYTGYGIIVTVFISLILVVLNLVKFDAEKATKAKLKIIVPESLNYPNAF
                     DQVFSKYLESYKLHKVKTMDFGTTFELTYYINMKSESNQKKFIDDLRVKNGNLNITMT
                     SEYVKSVTED"
     gene            complement(12528..14531)
                     /locus_tag="PLP02_03590"
     CDS             complement(12528..14531)
                     /locus_tag="PLP02_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPF42566.1"
                     /translation="MKKDYLIGKKRYVFYTLGLILYPTFVRSGNDSLFVNLIPIIIIG
                     SAVIFSIINHFFLYSMESNWYEEEIEMKHNDLFIAKGRKKIRVNLEYVNNPMIPIPSA
                     ASCGYLYSYYYNDGIGTSSLISHNNDSIIGAEKKENTKKAKKAIPDLLFNFDRRGFVR
                     FVTEETASTYGMKLEDFYGKNYHSLENLFGIDFDIWMEKLRSEGHCQSTVEVEEKGQR
                     IWIYWSFEAITDGKNEIEMIIATGHEITDIIVTGQVKDRIHDQLTGLLNQQGLYETLA
                     CLRNVRRATMFFIDLWNFTRINDYYGYHAGNEIIVKIADELKDWQDGNYIISRFSGDR
                     FIVVILNEKADSLEEMLANLRKMVSNTYRIGDQIIQIDKRIGYASYPDNTLQLDKLIP
                     MASLAMQQSKPENQFTIIRYERYMGEALKKNIVTATKLKKALDEHKIEVHFQKTVDSI
                     TKDAVYVEELARWNDSELGFVSPLVLFSVAKEFNLLEQLDKYMIEEAVRSFSIIRTKP
                     EYSKTKLTVNLAPGSFMDYRFLNYLNDSADKYHVEHSDICIEISEATFINSLDECNIR
                     IASYKNDNYLIALDDFGKEYSSLAILECVAFDIIKIDKIFIDHIFDKKNQEIIKMIKN
                     IADIGQKEIISEGVETKDQSDTLLNLGCPLQQGYFYHKPEKLT"
     gene            complement(14777..15340)
                     /locus_tag="PLP02_03595"
     CDS             complement(14777..15340)
                     /locus_tag="PLP02_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42567.1"
                     /translation="MYDYFMGRKSKDETMYLTAQLINYPLVLKQNFFTDIEVLILSFM
                     LSIVEKDQQKKLLKKLTSLFDTGENIMSGDNDAIYSLILMRLCKLHGVQKNYAKVVDL
                     CEKGIQRGTTYEYYYMLEYFFYYKALSHFKLEEYDSYEEALFRCYNVLHMDGNRKKIE
                     KFTKLIEKDFDINFDMFMMKYLKKNIL"
     gene            15795..15953
                     /locus_tag="PLP02_03600"
     CDS             15795..15953
                     /locus_tag="PLP02_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42568.1"
                     /translation="MKNLVRIFLAALVVMAFIGLGKAYMVQKAHVDNNQIVLVDFEDS
                     GEPEYIAR"
     gene            16004..16279
                     /locus_tag="PLP02_03605"
     CDS             16004..16279
                     /locus_tag="PLP02_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42569.1"
                     /translation="MQRQEFMVELGGIIGTIHTYTRNPFMVGYTTELKKAIRHNDVDT
                     TKIVLDKVIDWYSDEIDKINSDEYVFNKSMHRKAYGILLEYRQSLQA"
     gene            complement(16623..16706)
                     /locus_tag="PLP02_03610"
     tRNA            complement(16623..16706)
                     /locus_tag="PLP02_03610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16670..16672),aa:Leu,seq:cag)
     gene            16863..18356
                     /locus_tag="PLP02_03615"
     CDS             16863..18356
                     /locus_tag="PLP02_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF42570.1"
                     /translation="MKEDRQKKTPFVTKLEEYAMKNVSSFDVPGHKLGKIKTDLMDFT
                     GINTYALDSNAPIGLDTLGKPTGVIREAEKLMAEACHADKCYFLVNGTTVGILSMIMA
                     ICRANDKIILPRNVHKSVINALILSGAMPIFVKPDIDSELGIANGVSLETYQEAIEQH
                     PDAKALFIINPTYFGVVSEIEKITALAHEHDIVVLADEAHGTQFYFSDLLPISAMDAG
                     VDISASSFHKTCGSLTQSSVLLTKGGRIDHIRLEATLNMLQSTSPSSLLIASLDSSRK
                     TLYFEGKDRIAKLIAAAEKLRDNLRTVPGIKVVDRDYITERNGFDFDLTKIVVKVSDL
                     GLSGFEVYQKMRKNYNIQLELAESHIVLAVLTIGNTQDDLENLFFAFKDLSRRYYKIR
                     NRLPKIKFSYQFPETYVRPRDAYHAPKLEVPLAEAYDEISAESIMIYPPGIPLLIPGE
                     VITQDVIDDIEFYLKKGSVIHSELDNGQVRIIDKEHWIKWEGDDDDF"
     gene            18346..19206
                     /gene="speB"
                     /locus_tag="PLP02_03620"
     CDS             18346..19206
                     /gene="speB"
                     /locus_tag="PLP02_03620"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPF42571.1"
                     /translation="MIFEKVDLSFQSCKSKYEESDVVLFSAPMDATTSFRPGTRFAGN
                     VIRVDSIGVEWYSPYCDKSLKDYNTCDIGDLDLPIGDVEGALGEIYDVSSQIIKDGKK
                     TMMVGGEHLVTYPVVKAYHEKYPDLFLIHLDAHTDLREEFFGMKFSHATVIRRCHDLL
                     GDGRIYQYGIRSGDRDEFLFAKSGHVFQQKYDLDGIENLPEIIKNAPVYISIDLDILD
                     PSVFPGTGTPEPGGITFKELLGAFDVFAKLENVVGADIVELSPYLDPSGASNAAAAKA
                     LREMILILHK"
     gene            complement(19248..19538)
                     /locus_tag="PLP02_03625"
     CDS             complement(19248..19538)
                     /locus_tag="PLP02_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42572.1"
                     /translation="MIKHNRLTSIIYIGLVAVIDIVFAFFLPAEMVVQLHADGSPGTV
                     LPTWAALGLVFVIALAVAYQMYRKQKYEEIRYWFYTSIVVFVLNVILIVFNL"
     gene            complement(19535..20194)
                     /locus_tag="PLP02_03630"
     CDS             complement(19535..20194)
                     /locus_tag="PLP02_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42573.1"
                     /translation="MMRILENKRFILIFRIVFLIGILAGLVIMIIKREYPWQMFNYFS
                     LLSNIAVLAMILILVINRKKAGKWLIVAKQTVTVMILVTSILFFLTLLHYTASAFFGM
                     ITTWEYFFIYIFTPVMMLIDFILFDRVEKLVLRDSLWSLSACLLYFSFAEIFAVLDGV
                     ITYGIYYSFTLFDLSYFIRGGAVIQWICIAGIIAGVFVLSFGMDSLHNFLQQKKKEEV
                     V"
     gene            complement(20191..20724)
                     /locus_tag="PLP02_03635"
     CDS             complement(20191..20724)
                     /locus_tag="PLP02_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42574.1"
                     /translation="MMNAPVLLDKVHYKGYSVLHEYVTESYFRIAGNQDGTVFSLERE
                     AMSGPVRKQFEMTLYQDYVPNARAYGIFEEGILVAFIELDHETWNNRLRVTELYVIEA
                     LRRKGLGTKLIAFGIAEAKRLNARMLILETQTLNANAVSFYLKTGFFLCGFDLYSYSD
                     KDPSNHEVRLEMGMMIR"
     gene            complement(20721..>21442)
                     /locus_tag="PLP02_03640"
     CDS             complement(20721..>21442)
                     /locus_tag="PLP02_03640"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HPF42575.1"
                     /translation="MMGFFAGGFLLAGTAHARFKKISSSFKEKYMPSLIEKVLPGTQY
                     YPESGFAWEDVASMRFLKKSDRYHSEDYLIGTYKNIRFESADCKLQDVHSNGKTTTVV
                     TVFLGRVYRYEFPKNFPTELLLIQPGLGKKWGFGSYETIKTESIDFNKEFLTFARDEL
                     KAFEMLLPQFMEKLLELDRKYNDKIAVSFVGNTLNVAINNNIDTLEIRLFHPLDETVF
                     NEFESEIGDVKSLLDSLYGDK"
     assembly_gap    21443..21542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21550..22728)
                     /locus_tag="PLP02_03645"
     CDS             complement(21550..22728)
                     /locus_tag="PLP02_03645"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPF42576.1"
                     /translation="MGKLDYINELVEGLKKDGVYRTLPINYGPCGNVIDLNHHQVVNL
                     SSNNYLGLANDPRVKQAAIDAVNKYGAGTGSVRTIVGNQEPLEELEKLLAEFKHEEAV
                     VCFQSGLNCNIGVIQAVAQKGDLIISDQLNHASIIDGTRLSRADKAVFKHSDMEDLEN
                     VIKENRDKYAHILIITDGVFSMDGDLAKLPQIVELAEKYDCLTYVDDAHGSGVLGKSG
                     RGTVDHYNLNGRVDFIIGTLSKAIGVIGGYVATKQVVREWLLHRARPLLFSTALPPAA
                     IGAAIESVKLLMESEEYTNKLWDNARYFKEGMKKLGFDIGHSETPITPVMVGEEAKTM
                     EFSRELLKEGVFVSGIVFPTVPKGTGRLRVMISAMHEKKDLDFALKAFEKTGKKLGII
                     "
     gene            complement(22721..23815)
                     /gene="tdh"
                     /locus_tag="PLP02_03650"
     CDS             complement(22721..23815)
                     /gene="tdh"
                     /locus_tag="PLP02_03650"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPF42577.1"
                     /translation="MKIMTTSQELPDELTMMAVVKDKPERGFTYTQKKISTKLGPHEV
                     LIKVLTTSFCGTDYHIYDWNAWAQKRLKLPLTAGHEFSGEIIKIGTDVKRVKVGDIVS
                     AETHIICGECEFCKRGEGHICKNTKIIGVDTDGCFARYVKIPDANCFINDPGQNMLFL
                     SVQEPLGNAVHTIAHFPVKDKTVAIVGCGPIGIMGVDVAKALGAKLVIAIEITPYRQN
                     LARNIGADYVIDPTKEDVVSRVMEITDGAGVDVVGEFSGSSKAIQQAFKYIKKGGGMS
                     MLGIPDADIPIDVANDIVFKGLNIYGVTGRRIYDTWYQVRELLDSGKLHLDKIITHQF
                     PLKDIKIAAELMGEKNCGKIVMFPGDEIDG"
     gene            24037..>24775
                     /locus_tag="PLP02_03655"
     CDS             24037..>24775
                     /locus_tag="PLP02_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42578.1"
                     /translation="MNKANLFMKMEFLTKYSDLPATIVDDDNSILWCSFNQIDFDALP
                     IYPYVKDLEIKEGLPKLLIFNRIEIHGLFRIGSYRFLIGPAITAKPLTEIHYALLSAS
                     NCSQEISLDTIRNIPFVSICKFIQYLSLLCFIIDGKRFRTEDILQNKINVDTPALDNL
                     RSDFLPESTDRFGLIPQSNPDLREISEIYNSIKAGNEEEVARLIIDSSLAAKFKIASK
                     KENSAILGAAATLLAKAAFDAGVGFDDI"
BASE COUNT         6854 a         5619 c         5094 g         7108 t
ORIGIN      
        1 ttaagactca acaaatgatt acttttaatc gttcactata aacctttagg agtggtgcgg
       61 ttgatgggat ttgaacccac acgtccgatc ggacactacc ccctcaaagt agcgcgtctg
      121 cctgttccgc cacaaccgca acggcaatat ctattataaa gcattgacaa aaaaaatcaa
      181 gccaaaaata tccgatattg cgatgatgtc atttgaagcg ggaaaactgc tggatgccat
      241 ttttgtattt gggaggattt tccccgttga taagcggcac aatgtattca acaagctctt
      301 cgttcatacc atttccttca gagttgatca tcgaaagcgg gacccttttt tccgtatttg
      361 cacattccgt caaatcaaat aacccgtatt ccacttcgta tgggttgctg gatattctgt
      421 tgatggtgac cattttgccg gtgactccag caaccgcctg cctgacggcg tttttgccga
      481 cttctatcgc ttcggtgaca tcggtcagac tctgaatatg cgaagcggct ctttgcgtgg
      541 agcccaactc caccgagcgg gacggaattc ctaggtcagc agatatggtg gttgcgagtt
      601 tggaactgac gccgcctaat tggaaatgtc caaaagcgtc tttgaggccg gaacttgaac
      661 tgatgaagat tccttcttcg ttcattatgc cttccgaaac agcgaccaga cattgtttct
      721 tttcattgta tacttgtttg acatccttga gaaacttatc gacggaaaac ggtacttccg
      781 gcagataaat gagatcggga ccgaatcccg acaattgggc caaagcggat gatgccgtca
      841 gccagccggc atgacggccc atgatctcga cgatcgtgac tttgccgttc ggatatgcaa
      901 gcatgtcgta actaatctcc atcatcgtat tgatgatgta tttggccgcg cttccaaatc
      961 cgggggtatg atcggtatgg ggaagatcgt tgtcgatcgt tttaggaata ccgataatat
     1021 agcagtcgta atcgatatga ttcatataat cggcaatctt ggcgcaggtg tccatcgagt
     1081 cgttaccgcc attatacaaa aaataccgga tgttgtattt ttggaaaata tacaaaagac
     1141 ggaggtaatt ggaatcatcc tcacgaaagt ctttcaattt gtatctgacg gatccgaaag
     1201 cggatccagg ggtgtgtttc aacaattcga cggtttcttc gtcctggtcc ttcagaaaaa
     1261 agaaatcttc gttcaaggcg cctttgatcc cgtgtttcat gaagatgaca tccttgatgt
     1321 tcgggttttt tctggcttcg ctgataacgc cataagcgga agcgttgatt acgctcgtgg
     1381 gaccgcctga ttgtccgtaa agtaaattac ccttcaacat gtcctgactt acctctcttt
     1441 tgtttcgatt gccttttctt ccttcaacca gtaactactt ccgtaaggcg ttctttccat
     1501 tagaccgtaa tctatcaggc tgcgccttat cgtcgcgtaa tccggattga tctgttcaag
     1561 gatcgcgttt atctcttttt cagtataaca gattcccttt gtaaagcaac cagatacaat
     1621 ataaaaggcg acaaattgtt ttcgcctttt tgttggcatg accggcatcc gcaaaggatc
     1681 cttagatgga aaaaaacttt gcatgacggc ttcgatttct tcttgtgttg gtttttcgta
     1741 taacatacta gtctactcca tagcgacttc gcgtttcacg aaaatgtcat gattgagttc
     1801 cgatggctct gtattctgaa acacattgtc gagattgttt tgttcaaggt attctaggaa
     1861 ttcatccatt ttgcatagcg atgcaagccg cgaaaggtcg gaaattctcg gaagttcccg
     1921 cttgacgact tccatatgca tcgcgtcctg gaattcttgg acaggtgcga cagtgacaaa
     1981 atcgtatatt ttgacttttc cgtgaatttg gggataaagg gaaatattgc ggcgttgttg
     2041 ccaaattctt ggaatcgtcg gcgtcgacag catgtaaatc gctgccggag ccagactttc
     2101 gattccttcc gtgaagacat cgcttgtgat aataatatcg agcgcgccat tctggaatga
     2161 tttgatgatt cgaagccgtg attgcagatc aacttcgggt gtgaatacct cggtgttcac
     2221 accaaactgg aattccagtg tccgtttcaa ttcgtcagtt tgggtatgat ctacgtaaat
     2281 gatgactctt ttacgtacgg atgtcatgaa aatgcggaga aaatcaacgg tcttgttaaa
     2341 agctttttcc acaacacgtt ccttctgcaa tctgtataag gcggcttttt ccggcgattc
     2401 ggctctcaag gccatgccgg tcagttggcg gaactcggaa tattccggca cgatgagttc
     2461 gctcatgata acgttatatt cgtattcatt atgcgatttg ccaagcagat tcttcaaggg
     2521 ctggcttgac agttcgttgc ctatgatttg ttccaattta tcttttgaat cttcagaaag
     2581 ccatggcatc ggagttgatg aaagcccgat tctggcgcta ttatcaagat attcgcagtc
     2641 tttgaaatca ttgctttcaa ccaaaagatg gcattcatcg aatatataga acgaataatt
     2701 cttgttttta tggaaagccg cttgaaaatt ctcggaggcg aacactcggt atgaagcaaa
     2761 cacgaaaatg aagtcatctt tgagtttcaa acgcagaatt tctcccagtt cgacttccct
     2821 gacatcctct tcgtttgtca aaaaatatct ttgtttgccg ggatacttat ttgcgacaat
     2881 tttctcccat tcggcaatgg catgcaaatt gggagcgacg atcaaggcga ccgcacgggg
     2941 atatctgacg gcatactggt caagggcaaa catgcaggtc atcatttgat tacagccgtc
     3001 tggcaatgtg aatatccctt tccagtcaga tgatacgagc aaatcaatgg cttcttgctg
     3061 acttgcccgc atccggtatt tgacacgatc ttcgaacaaa tattcatacg ggcttgaaga
     3121 tacggccagg ctggcaaatt cggccctgac gctttgcgga agcggtacgc accatacgtt
     3181 gtcggctttg tttctccatt cgctttcgaa ctcgccttta taggaatcaa tcctcaaatg
     3241 atctctttgg tcccatgaca cgaaaatatc cgtggcatca agtggatcaa ccggtccgaa
     3301 aaacggtcca tggaaacaaa tggtatcctt accttccgat agaaaaatga tctggtctag
     3361 aggatactgg tcgtgtttcg gtaccgacac tacgatttcc aatattcccg ctgcgacaag
     3421 ataagacaga tattgcagaa aacgttgctc gtaaaacggc agatccatct tgaatctgtc
     3481 aacaatttct ttttttatta ttgactcgac ttcatcatcc cgatgaagca tgaaagcttc
     3541 cttttcgcgg ttgtcaagat gacaaagcga aacaatgaaa cgcattttcc cgccctggga
     3601 tacaaactgt aacaggctat ccttggaagc catgatccag tcatattcca aagaatacat
     3661 cctgacttcc accaattcgg atgttcttaa caaaggcaga aaattctttt cgaatccgtc
     3721 tttttcccga cgcgacaaat agataggcag tttgacgttc ttaatcggca tttttttcac
     3781 cttttttgaa tttcaaaatg aaaatgtttt ttctttgcta aacatataaa tatatttatt
     3841 ctttattatc attttttata gtaggtcatc cgaaacatgg ctaaatcggg catactatta
     3901 ttgtttatat tatatcatca tcttatagct ttgtattctg tttatttgat ttttatgtat
     3961 gaaaaagacg tttttaccat ttatttgata attttatgaa aataaatttg aaaaaaatat
     4021 taaataaacg acattttatg tcatttgtag tgtttgataa aattagccgg tataaaatcg
     4081 ttttttttcg ataaatgaca aattttgcat tctttatttg ctttagcata taatacagtc
     4141 cggcagagtc atcataagat tcttcactat tcttatattt gctgtttatg cataaaaaaa
     4201 gtttattcca tttctgaaat aaacctcgaa ttctctggag caagcgaggg gaatcgaacc
     4261 cccaacagtt gcttgggaag caacggtttt accattaaac tacacctgca ctctttgcga
     4321 taaataatat acaatgaaac cgttataaaa tccagcgttt ttttcgaaaa acgtcgtata
     4381 taataaaaaa ataaggatgc cctgcggaca tcctcatttc attcattctg ttttgttttt
     4441 cgatttttcc aagatatcgc caaacagttt tctcacttgt ttgtcttcga ggaacggtag
     4501 gagatcatcg accgcgagct tatcgttttc tgtctcttgt agttcgttaa gcaactcatc
     4561 gatctgttct tcttccaaaa acggcagaat cccctttggc gaaacgttta tcgttccttc
     4621 catagcgctt cttaaaaccg tcatcagttg ttccggttca aggaaggggt acagataagg
     4681 caccttgaaa tcttcttccg taatctcgcc ttttacacat gcttccaaaa gactgttgag
     4741 atcctctttg cacagatatg gcaacatttt cttgatattc attttcttac ctccataatt
     4801 ttcttttttc cgcatcggtc atcattgaat tgatgcgatc gatctgcaac ggatattttt
     4861 tatccctgta cttgtttatc aggaaggccc tttcatcgta tgacagcctt ggccataggt
     4921 tgtcgccaat ttcgattcct tcctccgtca tcaggtacaa agcgtattcc ctttcggtct
     4981 gactcagcaa gtgaatgatc ttttccaatc cgacgccttt gatttttcct gtcgccgtct
     5041 cgtgtatcac ccagtcacgg gaatgttctc ggaaaagttc ttctccgccc ttgggggtaa
     5101 tgctttcata taaacaaaga atttcttcca cgcttgtatt gtaaaacgaa gtcatttccg
     5161 ccaatgtgtc gaccgatggc aaggcttttc cggtttccca cttggaaacc gcctgatgtg
     5221 acactaacag aacattggca agatcttcct gcgtcatatt ctttttttgt ctcaattcgc
     5281 atagtttgtt tccaattttt accatgtcga gcattccttg tcaccttctc tcttccggat
     5341 ccgtcaaaca tagtatatgc gattttaaga aaaagataaa gcaactggtg gtagaaatgc
     5401 ttcaaacgtg ctttttgaca tatgttactt aatatataag gttgacaaaa accgctaaat
     5461 agcggataat agtattgtta aaaacgacgt ttttaaagga ggattaccta gtaaaaatca
     5521 ataacccttg taatattgtc aagtccataa tactctttcg gtattaagcc ggactgaaca
     5581 attcgaaaag gattaaagcc ttttacacat atgcttgata caaccttttc acgaaaagct
     5641 tcgtttgctt tttttttgtt cggtccgaca aaaatatata attattgaga acaggagaga
     5701 aatatgtcat taaaaaacat cttggaaaag tatccccgaa atgaatccag cctgattaaa
     5761 attctgttgg aatttcagga tacgaagaaa gacaacagca tctctgaatc ggaagtcaag
     5821 gaaatatcag attaccttgg cgttcctgaa agtaaaattt gcagcgtgat ttccttctat
     5881 tcgttcttct ctttcaatcc ccgcggcaaa tatgtcatcc ttgtttgcaa ggatgttccg
     5941 tgttttgtca attcccaggt ctccatcaag gatgctctcg aggaatcgct cggaatcaaa
     6001 atgggagaaa cgacaaaaga cggtcttttc acgctggaat acaccggatg tctcggttgt
     6061 tgcgacaatc ccccggctat tgaaatctcc gggaagacat attgcggtct gactttaaaa
     6121 aaggtcagag aaatcattga tgaatacagg aggatggcat aatgttggaa atgaaactgg
     6181 tatccaagcg ttgcggagtc atcgacccgc tttcaatcaa agattatatc gacaacggcg
     6241 gatacaaagc gttggcaaaa gctttggccg atggaaaaga gaaaacaatc tctgaaatcc
     6301 aggcttcaaa agttcggggc cgcggcggcg ccggctttcc tctagctatt aaaatctcat
     6361 cagttgccaa agaaatagcc gatgtcaaat atcttgtctg caatgccgac gaaggcgaac
     6421 caggaaactt caaggatcgc tatcttatgg aaaacgatcc tcatggcatg atcgaaggca
     6481 tggtcattga cgcttatctt accggagcca ctaagggata catatatatc cgcggcgaat
     6541 atcagaaagc ccaaaaaata gttgaaaccg ctctcgaagc cgccaaaaac gccaactatc
     6601 tcggcaaaaa catttgcgga gcaggattcg atttcgacct cgaaattcgt tccggtgccg
     6661 gttcatacgt ttgtggtgaa gaattcgctt tgctggaatc aatcgaagga aaacccggac
     6721 gcagtcgcgt taaacccccg ttcccgaccc aaaaaggttt gtttggtaaa ccgactcaga
     6781 tcaacaacgt cgaaacattc gcaactatcc ccgtcatcat tctcatgggc ggaagcgaat
     6841 atcagaagat tggcactcca acttctaccg gaaccaaact tgtttcgtta tgcggaaacg
     6901 tcaggaacaa aggcgtttat gaaataccgt tcggactatc cttacgcgaa gttatcgata
     6961 aactcggtgg cggagtcgat ggtggccata aaatcagaat ggttcaacta ggtggagcat
     7021 ctggtccgat cattccggaa agcatgatcg acctttcgat cgattttgac catttcgatg
     7081 ttttctcagc taaagccgga tccggcgccc tgatcgtcat tgacgaacgt gcggacatgt
     7141 tcgaaatctt acaaagaatc atggaattct ttgttcatga atcttgcgga aaatgcactc
     7201 cctgtcggga aggcaatctc caactcttaa agatcatcaa tcgtttcgtg gaaggcaaag
     7261 gccgtaaatc cgatcttgaa actctttcca tattgacaaa cgtcatgaaa aacacgtcgc
     7321 tttgcggtct cggacaagca tcgccgacag caatcatttc gacaatgcgt tatttcccgg
     7381 acgaatatga aaaacgtctg gcgaaagacg aaatgtgagg aggcgcacca tgataaattt
     7441 gaaaatcaac aatatcgaag tcagtgttcc ggaaggaaca acgattcttg aagcttccag
     7501 agccaacgga atcaatatcc cgacattatg caattatccc gatctcggca tcaaagccga
     7561 atgccgggtc tgcgtcgtcg aaatcgaagg ccgtcgcggt ttgtcgacat cctgctccac
     7621 catatgtgaa gacggtatgg tagtaaaaac caacacaccg gatgtcatca ccgccagaag
     7681 aacgatcact caactgcttc ttgccgacca cgatgtcgat tgcccgagtt gcccgaaaaa
     7741 tctgaactgt gaactacagg aacttgccaa ccgactcaat atcgtcgaca atccttttga
     7801 ccatgtggaa tttccgcaac ctgtcgatga agacaatcct tcgatcatcc gcaatcccaa
     7861 ccgttgcata aaatgcggac gctgcatcga agtttgccgc gaagtccagg gaatcgatgt
     7921 tctcgaatac atgggccgcg gtgtttccga agccattacg ccggctttcg gccgtaaact
     7981 caatgacctc ttctgcactt actgcggaca atgcacgagc gtttgcccgg ttggtgccat
     8041 cgttgaaaag gatgataccg acttggtctt ccaagcgatc aacgatcctt cgaaacatgt
     8101 gatagtccaa accgctcctg ccatcagggt ttccctgggc gaagaaaatg gaatgaacgc
     8161 cgggacggtt gtcaccggaa aactggtttc cgctttgaga gctctcggtt ttgacgaagt
     8221 gttcgacacc gatttcaccg ccgacctcac gattatcgaa gaaggaaacg aactgatcca
     8281 acgcattacc acgggcggcg tgctaccgat gatgacgtct tgctgcccgg gatggatcaa
     8341 ttttgccgaa caggaattcc cggaaattct tccgaatatc tccagctgca agtcgccgca
     8401 acaaatgttc ggcgccttgg ccaaatcata ttacgccgaa aagatcggtg ttaacccgaa
     8461 agatttgttc gttgtttcga tcatgccttg tacagccaag aaatatgagt gcaaacgcga
     8521 agaaatgcaa gttaataagg gtattcagga tgttgatgtc gttctcacaa cccgcgaact
     8581 cggcaaaatg atcagacagg tcggaatcga ctttgaaaat ctcaaggaat ccgaattcga
     8641 cgctcccttc ggcatcacca ccggagccgc cgccatcttc ggcgccaccg gcggtgtcat
     8701 ggaagccgcc gtcagaacgg tttacgaagt cgtcaacaag gaagaactga aaagcctcga
     8761 tttcaaaggc ctgcgtggtc ttgacggaat caaagaagca actgtcacgc ttggcggtca
     8821 ggaagtcaaa gtcgcagtat gccacacatt gtctaaagcc cgcattttgg ccgaacaggt
     8881 aaaaaatggc acttccccct atgctttcat agaagttatg gcatgcccgg gcggatgcat
     8941 cggcggcggc ggacaacctt acggcactgt gaacaagatc cgttcgaaac gtatcgatgc
     9001 cacctataat gtcgacaaag acatgccgat ccgcaaatcg cacgagaatc ccgccatcaa
     9061 atccatttac gagaattacc ttgaaaaacc gctcggtcat aagagccatc atttacttca
     9121 tacgacttac acttccagaa ataaaaactg acaatatcat tactatatat catagaaaag
     9181 gccgggaatc actttccggc ctttcgctta aatttatcac cattacggat ttaattcatt
     9241 aaaacaacac aatctaaaca tataaatgca ataattccat tgtataatca tacatatcca
     9301 agttacacat tcttactaac gggagaccat catgaaaaga aacatcggtt tcgcttttct
     9361 tttgattatc gccttgttgt tatcaggctg cacgcttgac ggatcagata ccgcaattat
     9421 cactaaccaa ttgaattccg acgacactcg ggtatctttc gatacttttg acgacaccat
     9481 cactttttat aacacggaaa taacttcgac aaacggagtt gtaaatataa gccaaaatat
     9541 cgtcacgata agcactgccg gtgtttatct tttttccggt tcgtctttga acgccagaat
     9601 agtggttaat gctggcgatg aagataacgt ctttctcgtc ttgtccggtt tggatctgac
     9661 atcttccgaa gatgctccga taacgataac aaatgccgac aaagtcatca taaatcttgt
     9721 cgaaggaacc ataaacacgc tgacggattc cgcttcttcc ttgaacgatc attcagcttg
     9781 tatctctagt tcggacgatc tatccataaa tggttccggc tcccttacga taaatgccaa
     9841 ttacaagaac ggtattgatt ccaacgacga tctggtgatc gccggtggca acatcgcgat
     9901 tttagccgat aatgatgcta ttaaagccaa taattcattt accatgacag aaacgacgct
     9961 aacgttagaa tccgacaatg acggaatcca cgtggaaagc gatgaatcat ctctcggaaa
    10021 tgttttactt atttccggca cactttccgt ttcgtcatat ggcgatggca tagatgcgag
    10081 taaccaggtt gttatatcat ccggaacata caatcttgtt tccggggtag gcaactccaa
    10141 tatcgactct gtcagcggaa aaggcataaa agcgacttcc ttgatcatga tcaaggacgg
    10201 agagttcaca atcaactcca aggacgactc gatccactcg aatgacgatt tacggataac
    10261 tggaggcaac tttgatatct attctggcga cgatggcatt cattccgaca cttcattgga
    10321 aatatccgga ggcacattca acattgccag agcctatgaa ggcatcgaat cgctttcgct
    10381 cgaaatttcc ggaggtttca tttacatcaa ctctagcgac gatggaatca acggcgcgga
    10441 cggtacggat tcttccggca ctttcgcttg ggatccgcat gcgggaggag gaatgtcatc
    10501 cggcaacgct tctttgaaaa tcaccggcgg aactatttat atcaattcaa ttggcgacgg
    10561 cgtagacata aatggatcga tttccatgtc ttcgggagca ttgcttgtgg ccggtccgac
    10621 atcaagcgac aacggcgcca tcgattatga cgggtcgttt acgatcaatg gcggcttgct
    10681 ggtatgcgcc ggatcatcgg gaatggcgca aaacgtcagt tccggttctt cccagacaac
    10741 cgttctcatc acattgtcct ccacgacttc cagaatgttc cacctggaaa gcgcagacgg
    10801 agaaaacatt gccactctgt ctcctggtaa atcataccaa tctattctcg tatcatctcc
    10861 aaaacttaat gtcggttctt catacacaat atatatcggc ggcgatatga ctgatttcgc
    10921 caccgaaata tacggatatt atgacggcgg cacctatgtc gcaggaaccg aatatgcgac
    10981 cttctcgata agccagacca tttccacagt cggaagcagc atgtacaggt gatcgccatg
    11041 agccaaagcg tattcacaag atatgaggaa aagtacttgc ttacggaaaa gcagaaaaat
    11101 gacatgtttg gctgtcttgc ccattatctg ccttttgatt cgcattctcc gaacggagca
    11161 agttacaatg tctacagtct gtatttcgat acttccgaca acaatgtcat cagacaatcc
    11221 ctggaaaaac caaagttcaa ggaaaagctg cggatcaggt gttatgaacc gccaaagaat
    11281 gactcggatc ccgtttttct cgagattaag aaaaaggatc atggtatcat aaacaaaagc
    11341 cgcatcgtca taggatacgg caaagcgttg gaatttgtgg cgacaaaaca gataccggct
    11401 tttctatcgg acgactactt cactaaccga gtcgcaaacg aaatagagta catgctggaa
    11461 cagaacacgt tgacgccaag ctacttcatc tgttacaaga gacaggcttt cgcagctagg
    11521 gaaacatact tccgcgtaac attcgatcac gacatcttat acagaaacac cgaaccttcc
    11581 ttattggatc tggaaggaca gccggtcatt ccggcagaca aatggctgat ggaaatcaaa
    11641 gccgaaaaca attatccgtt atggctcgtc cgcaaattat ccgaactggg gctcgcaaat
    11701 caaagtttct ccaagtttgg ccgggcttac caaaatacac taaaaggaga attttgatat
    11761 gttactaagt attttcgttc ttgacactga aaccctgact cttggcaatg tcgtgcttgt
    11821 catgcttttc gccaccatcc tcggagccgt gcttaccggc atttacgccc tgacgcacag
    11881 actgcttggc tttgaccgtt cgttttgcgt aacccttctg atgttaccga taattgtttc
    11941 catcatcatc atgcttgtat ccaacaacat cgcgagggct ttcagcttgg ctggcgtgtt
    12001 caccctgatc aggttcagat caacgcttcc ggatactcgc gatatcacct atgtattcgc
    12061 ctccgtggcg atcggcctaa tcgtcggcat gggatacacc ggctacggta tcattgtaac
    12121 tgtattcatt tctttgattc tcgtcgttct caacctggtc aaattcgatg cggaaaaggc
    12181 gaccaaagcc aaactgaaaa taatcgttcc cgagagtctt aattatccga atgctttcga
    12241 tcaggttttc agtaaatact tggaaagcta caagcttcac aaggtcaaaa caatggattt
    12301 cgggaccact ttcgaactga cgtattacat caatatgaaa agcgaaagca atcaaaagaa
    12361 gtttatcgac gatttacggg tcaaaaacgg gaatctcaac atcaccatga ccagcgaata
    12421 tgtcaagtcg gtgacagaag actaaaaaag aataaaaaaa tatcacaccg ggaatgtgat
    12481 atttttttga tacattgcca aatataaagg aagcgcctgt gcttttatca tgtaagtttt
    12541 tctggcttgt gatagaaata tccttgctga agcggacagc caagattcaa cagggtatcg
    12601 ctttgatcct tggtctccac tccttcggag attatctctt tctgaccgat atcagcgatg
    12661 ttcttgatca tcttgatgat ttcctgattc ttcttatcga agatatggtc gatgaatatc
    12721 ttgtcgatct tgatgatatc gaaagcaacg cattccagaa tcgccagaga cgagtattcc
    12781 ttgccaaaat cgtccagggc tatcaggtaa ttgtcatttt tgtaggaagc gattcggatg
    12841 ttgcattcgt caagactgtt gatgaatgtc gcctcggaga tctcaatgca gatatcgcta
    12901 tgctcgacat ggtatttatc cgccgagtca ttcaaataat tcaggaatct gtaatccata
    12961 aatgatccgg gagccaggtt cacggtgagt ttggtcttgc tgtactccgg ttttgtcctg
    13021 atgatcgaga atgatctgac ggcttcttct atcatgtatt tgtcaagctg ttcaagcaag
    13081 ttgaattcct tggcaactga gaaaaggacg agcggagata cgaatccgag ttcggaatca
    13141 ttccaacgag caagttcttc gacataaacg gcatccttgg tgatcgaatc aacagtcttc
    13201 tggaagtgaa cctcgatttt atgttcatcc agggcttttt ttagtttggt tgccgttacg
    13261 atgtttttct tcagggcttc gcccatgtat ctttcataac gaatgatggt aaattgattc
    13321 tccggtttgc tttgctgcat cgccagcgat gccatcggaa taagtttgtc aagttgtaga
    13381 gtgttgtccg gatatgaagc ataaccgatc cgcttgtcaa tctggatgat ctgatcaccg
    13441 attctgtatg tgttcgaaac cattttgcga agatttgcaa gcatctcttc taacgaatct
    13501 gctttctcat tcagtatgac aacaatgaaa cgatctccag aaaaacggga aattatataa
    13561 ttgccatcct gccaatcctt gagttcgtcg gctatcttca cgattatctc gttgccggcg
    13621 tggtagccgt aataatcatt gattctggtg aaattccaca gatcgataaa gaacattgtt
    13681 gctcttctca cattgcgtag acacgctaat gtttcataaa gtccttgctg gttaagaagt
    13741 ccggtcagct gatcgtggat tcgatccttg acctgtcctg taacgataat gtctgtgatc
    13801 tcatgaccgg tagcgattat catttcgatt tcgttcttcc cgtccgtaat cgcttcaaaa
    13861 ctccagtata tccagatcct ttgtcctttt tcttcgactt cgacagtact ctggcaatgg
    13921 ccttccgatc taagtttttc catccatata tcgaaatcaa ttccgaagag gttttccaac
    13981 gaatgataat tctttccgta aaaatcctca agtttcatac cgtaagtaga agctgtttct
    14041 tcggtaacga atctgacaaa gcctcttctg tcaaaattga ataacaggtc aggaatggct
    14101 tttttggctt ttttggtgtt ttctttcttt tcggcaccga taatactatc attgttatgc
    14161 gatatcagcg acgaagtccc gataccgtca ttgtaatagt atgaatacaa gtatccgcaa
    14221 gatgcagcgc ttggaatcgg gatcatcgga ttgttaacgt attccagatt gacccggatt
    14281 ttttttctgc ctttggcgat gaacagatcg ttgtgtttca tttcgatttc ttcctcgtac
    14341 cagttcgatt ccatggaata caggaagaag tgattgatga tcgagaagat aactgctgag
    14401 ccgatgatga taatcggaat caaattgaca aacaggctgt cgtttcctga acgcacaaaa
    14461 gtgggataca gtatcaatcc caacgtgtag aaaacataac gctttttgcc gataagataa
    14521 tctttcttca tgtcgatcac tcatttccga attctattga atgatagtcc ggaaaatctc
    14581 cattgtattc attgacgtat ttttctactt ttcggcttca gcatagccaa aaacagatca
    14641 ttacttccct ttttaattgt tctccattat tattatataa ttagtaaata ccttaaaaca
    14701 gcgcggatat gtcgtacttt ttgacataat gaaaaaaaat cggccttttt tgaccgattt
    14761 tctgaggttt ttttatttat aaaatgtttt tcttcaggta tttcatcatg aacatgtcga
    14821 agttgatgtc gaagtccttt tcaatcagtt ttgtgaactt ttcaatcttt ttcctgtttc
    14881 cgtccatatg caggacatta tagcacctga acagagcttc ttcgtaagaa tcatactctt
    14941 ctagcttgaa atgtgagagc gccttatagt aaaaaaagta ttctagcata taatagtatt
    15001 cgtaagtcgt gcctctctga ataccctttt cgcacaaatc tacaactttg gcgtaatttt
    15061 tctgcactcc gtgaagtttg caaaggcgca tcaggatcag agaataaatc gcatcattgt
    15121 ctccgctcat gatgttttct cctgtatcga aaagggaagt caacttctta agaagtttct
    15181 tttgctgatc tttctccacg atactgagca taaagcttaa aatcaacact tcgatatccg
    15241 taaaaaagtt ttgcttgaga actagcggat aattgatcaa ttgggcagtc aaatacatcg
    15301 tctcgtcctt ggattttctt cccatgaagt aatcgtacat gatgagagcg tgctggaagt
    15361 ataactttct atcttcgttt aaaatgaagt ctttttcaag aatttgctta atatcttgcg
    15421 ccttgtgtaa atctttatta acaacggcgt tatagaaatc gtttatttgc ttatattgaa
    15481 gtgaactctc cctttcaaat tcatgcatta gcttttttga tggaattcct aatttatttg
    15541 cgaattgttc aattttttcg tatgttatct cacattctcc gctccggtac ctttgatatt
    15601 gtcgcaaaga aacaactccg tcgacaaaat cttcctgtga gatttttctc ccgtagcgta
    15661 ttttttccaa ataattgcta atttccatag aattcatgca ttatcaccca tttttgccaa
    15721 aaaatacgac atatctgcgc tgttttttat attttttttg cgttatcata taagcgaaaa
    15781 gggaggtatt aattatgaaa aacttggtta gaatcttttt agcagctctc gtggtaatgg
    15841 cattcatcgg cttgggaaaa gcctatatgg ttcaaaaagc acatgtagat aacaatcaga
    15901 tcgtcttggt tgacttcgaa gattccggcg aacccgaata catcgctcgc taattattca
    15961 ttagcgaaga tcatcgaaat ctagcaaagg acggtaaaaa agtatgcaga gacaagaatt
    16021 catggttgaa ctaggtggaa taatcggaac gatacacacc tatactagaa atccgttcat
    16081 ggtaggctat acaacggaat tgaagaaggc tatacgccat aacgatgtcg acaccaccaa
    16141 aatcgtgctt gacaaggtga ttgactggta tagcgatgaa atcgacaaga tcaacagcga
    16201 tgaatatgtt ttcaacaaga gcatgcacag aaaagcgtat ggaatcttgt tggaatacag
    16261 acagtctttg caggcataag ttgatttccg gttttgagtt gccggtcctt ctgacataat
    16321 ctactaacat tttcctcaag aaacattata acacgaatca ttttccaaaa acactatatt
    16381 tgcacccttt tattctccga taactgtttt ccctgaaaag cgaagaaatg tcttgccccc
    16441 atcgatatgg caggattttc ttcgcttttc ctataaaaac ataattataa gataaaaaaa
    16501 agacggaaaa gccatttgct ttttccgtct tgattttagc tggcagggct agagagattc
    16561 gaactctcgg tctcggagtc agagtccgat gccttaacca cttggctata gcccaatcgg
    16621 tctggtgcgg ttgatgggat tcgaacccac acgtcgttga cactaccgcc tgaagatagc
    16681 gcgtctgcca gttccgccac aaccgcatag ataccgcatt agttattata tcataatcta
    16741 tgaaaaagaa aaggaaaaaa ttcttccgaa aatccaaata ttatactttt gtatttgtta
    16801 gatatatgtt attattcatt atgcattttg atacttgcta cggaaggcgg tcataacgaa
    16861 ttgtgaagga agacagacag aaaaaaacac cttttgtaac caaacttgaa gagtacgcca
    16921 tgaagaacgt atcctcattc gacgtacccg gccacaaact tggcaaaata aaaaccgact
    16981 tgatggattt taccggtata aacacatatg cccttgattc gaacgccccg atcggacttg
    17041 acactttagg aaaaccgacc ggagtcatca gggaagccga gaaactcatg gctgaagcct
    17101 gtcatgcgga caaatgctat tttctggtca acggaaccac tgtcggcatt ttgtcgatga
    17161 tcatggcgat atgccgtgcc aacgataaaa taattcttcc gcgcaacgtc cacaaatccg
    17221 tcatcaacgc tcttattcta agcggagcca tgccgatatt cgtcaaaccg gatatcgatt
    17281 ccgaactcgg aatcgcaaac ggtgtcagcc tggaaaccta tcaggaagct attgaacaac
    17341 atccggacgc caaagcgctt ttcattataa atcccactta tttcggagta gtatcggaaa
    17401 ttgaaaagat cactgcgctt gcccacgaac acgatatagt cgtgttggcg gatgaagcgc
    17461 acggaaccca gttttatttc tccgatcttc ttcccataag tgccatggat gccggcgtcg
    17521 acatcagcgc ttcttctttt cacaaaactt gcgggtccct tacgcagagt tcggttcttc
    17581 ttaccaaagg cggccggatc gaccacatca gattggaagc gacattgaac atgctgcaaa
    17641 gcacatcgcc aagttcgcta ttgatcgcaa gtcttgattc atcccgcaaa accctttatt
    17701 tcgagggaaa ggaccgaata gccaagctta ttgccgccgc tgaaaaactt cgtgacaatc
    17761 tgcggacagt cccgggaatc aaagtggtag accgcgatta catcaccgaa agaaacggct
    17821 ttgatttcga tctcacaaaa attgttgtca aagtatctga tctaggtctt tccggattcg
    17881 aagtctacca gaaaatgcgt aaaaactaca acatccagct ggaactggcg gaatcacata
    17941 tcgttttggc tgttctgact atcggaaaca cccaggatga tctcgaaaat cttttctttg
    18001 cattcaagga tttatcccgc cggtattata aaatcagaaa ccggctacct aaaattaaat
    18061 tttcctatca gtttccggaa acatacgtcc gtccgcgcga tgcctatcac gctccgaaac
    18121 tggaggtccc attggcggaa gcctacgatg aaatcagtgc cgaatccatc atgatttatc
    18181 ctccgggtat cccgcttttg attccggggg aagtcattac ccaggatgtg atcgacgata
    18241 tcgaattcta tttgaaaaaa ggatcggtga tccatagcga actagacaac ggtcaggtcc
    18301 ggatcatcga caaggaacat tggataaaat gggaaggtga cgacgatgat ttttgaaaaa
    18361 gttgatcttt cgtttcaaag ttgcaaatcg aaatacgagg aatctgatgt cgttcttttc
    18421 agcgcgccca tggatgcgac cacttctttc cgtccgggca caaggttcgc cggtaatgtt
    18481 ataagagtcg attccatcgg agtcgagtgg tacagtccgt attgcgacaa aagcctgaaa
    18541 gactacaata catgcgatat tggtgatctc gatctgccga tcggagatgt ggaaggcgcc
    18601 ctcggagaaa tctatgacgt ttccagccaa ataatcaaag acggcaagaa aaccatgatg
    18661 gtgggcggag aacatctggt gacatatccg gtggtaaaag cctaccatga aaaatatccg
    18721 gatctgtttc tgattcatct tgatgcccac acggatctac gcgaagagtt cttcggcatg
    18781 aagttttccc acgcaaccgt catcagacgc tgccacgacc ttctgggcga tggccgcatc
    18841 tatcaatacg gcatcagaag cggagacaga gacgaattcc tatttgcgaa aagcggtcat
    18901 gttttccaac aaaaatacga tctcgacgga attgaaaatt tacctgaaat aatcaaaaac
    18961 gccccggtgt atatctcgat cgatctggat atactcgatc cgtcggtctt tccgggtacc
    19021 ggcactcccg aacccggcgg catcacgttc aaggaacttc ttggagcctt cgatgtcttt
    19081 gccaaactgg aaaatgtcgt tggagccgac attgtggaac tttctcccta tcttgatccg
    19141 tcaggcgcat ccaatgccgc agccgccaaa gccctcaggg aaatgattct gatcctgcat
    19201 aaataaaaac agccaaatgg ctgttttttt tgtattcgta accgaatcta cagattgaat
    19261 actatcagaa tgacattcaa tacgaaaaca acgatcgagg tgtaaaacca gtaacggatc
    19321 tcctcgtatt tttgttttct gtacatctgg taagctaccg ccaatgcgat cacgaacacg
    19381 agaccgagtg cagcccaagt cggaagcaca gttccgggcg agccgtccgc atgcaattga
    19441 actaccattt ctgcgggaag aaagaatgcg aaaacaatat cgatcactgc gacaagtccg
    19501 atatagatga tagaagtcag tctgttatgt ttgatcatac tacttcctcc ttttttttct
    19561 gttgcaggaa attatggaga gaatccattc cgaaagaaag cacaaatact ccggcaatga
    19621 ttccggcaat gcaaatccac tggataacag ctccgccccg tataaaatat gataaatcga
    19681 atagagtaaa cgaatagtat atcccgtaag ttataacacc gtccaacaca gcgaatattt
    19741 cggcaaagga gaaatagaga agacacgcgg aaaggctcca aagagaatct ctcaaaacta
    19801 gtttttccac cctgtcgaaa agaataaaat cgatcagcat catcactggc gtaaatatat
    19861 agataaagaa atattcccat gtcgtaatca ttccgaagaa cgcggatgca gtataatgca
    19921 ataaagtgag aaagaaaaga atggatgtca ccaatatcat cactgtcacg gtttgtttgg
    19981 ccacaatcag ccattttccc gctttttttc tgtttattac aagaatcaat atcattgcga
    20041 gtacagcaat attgctaagt aaagaaaagt aattgaacat ctgccaagga tattcccttt
    20101 tgattatcat aatcactaag cctgccagaa tgccaattaa aaacacgata cgaaaaataa
    20161 gaataaaacg cttgttttcc agtattctca tcacctgatc atcatcccca tttccaatct
    20221 tacttcatga tttgacggat ctttgtcgga atatgaataa agatcgaatc cgcaaaggaa
    20281 aaagccggtc ttcagataaa acgaaacagc gttggcgtta agcgtctggg tttcgagaat
    20341 gagcattctc gcattcagcc ttttcgcttc cgcgatgcca aacgcgatca gttttgttcc
    20401 gagacccttt cttctcagcg cttcaatcac atataattca gtgacccgga gacgattatt
    20461 ccaggtttca tgatccagtt cgataaaagc taccagtatg ccttcttcga atattccata
    20521 cgctctggca tttggaacat aatcctggta aagagtcatt tcgaattgct tcctaaccgg
    20581 tccggacatt gcttcccttt cgagggaaaa taccgttccg tcctggtttc ccgcaatcct
    20641 gaagtaagac tccgtcacgt attcatgcaa aacggaatag cctttgtaat gcactttgtc
    20701 aagcaaaaca ggagcattca tcatttgtcc ccatacaaac tgtccagtaa acttttcaca
    20761 tcgccgattt ccgactcgaa ctcgttgaac accgtttcgt caagcggatg gaacaatctg
    20821 atttccaggg tatctatatt gttgtttatg gcgacattca aagtgtttcc cacaaaggac
    20881 acggcaattt tgtcgttgta tttccggtcg agttccaaca acttttccat gaactggggc
    20941 aaaagcatct cgaatgcttt cagttcgtct ctggcaaagg tcagaaattc cttgttaaaa
    21001 tcgattgatt cggtcttgat cgtttcataa ctgccgaatc cccatttctt acctaatcca
    21061 ggttggatca ataataattc cgtcgggaag tttttcggga attcatatct atacacacgt
    21121 ccgagaaata ccgtgacgac agtggttgtc ttaccgttcg aatggacatc ctgaagtttg
    21181 caatcggctg attcgaatct gatattctta tatgttccga tcagataatc ctccgaatga
    21241 tagcgatcag atttttttag gaaacgcatg gatgccacgt cttcccaggc aaagccgctc
    21301 tccggataat actgggtgcc cggcaatact ttttcgatta acgacggcat gtacttttcc
    21361 ttgaacgagg agctgatttt tttaaatctg gcatgcgcgg ttccggccaa aaggaaaccg
    21421 ccagcaaaaa agcccatcat cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnagcaagtt cagatgattc ccagtttctt gccggttttc tcgaatgcct tcaatgcgaa
    21601 gtcaagatct tttttctcat gcatggccga aatcatgacc cttagtcttc cggttccttt
    21661 cggaacggtc gggaagacga tgccggaaac aaagacgcct tcttttaaca attcgcgtga
    21721 gaattccatc gttttggctt cttctcccac catgacaggc gtgataggag tttcgctgtg
    21781 tccgatgtcg aaaccgagtt tcttcatgcc ttccttgaag tatctggcat tgtcccaaag
    21841 tttgttggta tattcttcgc tttccatcag cagtttgacg gattcgatcg ccgctccgat
    21901 tgccgcagga ggcaatgcgg tcgagaaaag caaaggacgc gcccggtgaa gcaaccattc
    21961 gcgaaccact tgtttcgttg cgacatatcc gccgatgaca ccgattgctt tggacagagt
    22021 tccgatgatg aaatcgacac ggccgttaag attgtaatgg tcgacggttc cgcggcctga
    22081 tttgccaaga acaccgctac catgggcatc gtctacatag gttaggcagt cgtatttttc
    22141 cgccagttcg acgatttgcg gtaatttcgc cagatcgccg tccatcgaga agacaccgtc
    22201 ggtgatgatc aaaatatgag cgtatttgtc acggttctcc ttgatgacat tttccaagtc
    22261 ttccatatcg gaatgtttga aaacggcctt atcggctctg gagagtctgg tgccgtcgat
    22321 aatcgaagca tggttcagct ggtcgcttat aatgaggtct cccttttggg caactgcctg
    22381 gattacgccg atattgcagt ttaatccaga ttggaagcac acgacggctt cctcgtgctt
    22441 gaattcagcc agaagctttt cgagttcttc tagtggttcc tgattgccga cgatcgttct
    22501 gacgctgccg gtgccggcgc cgtatttgtt tacggcatcg atcgcggctt gcttgaccct
    22561 tggatcattc gccaagccta gataattgtt agaagaaagg ttcacaacct gatgatgatt
    22621 caaatcgatg acattgccac aggggccgta attgatcgga agggtccggt atacaccgtc
    22681 ctttttcagt ccttccacca gttcgttgat ataatccaat ttacccatct atttcatctc
    22741 ccgggaacat tacgattttg ccgcagtttt tttctcccat cagctcggcg gcaattttga
    22801 tatctttcaa ggggaactga tgtgttatga ttttgtcgag atgaagcttt ccgctgtcca
    22861 gaagttctct cacctgatac caggtatcgt agattcgtct tccggtcacg ccgtaaatat
    22921 tcaggccttt gaacacgatg tcattggcga catcgatcgg aatgtccgcg tccgggattc
    22981 cgagcatcga cattcctcca ccttttttga tgtatttaaa tgcctgctgg attgctttgc
    23041 tgctaccgga aaattctcct acaacgtcca ctccggcacc atctgtgatt tccatgactc
    23101 tggatacaac atcttccttt gtcggatcga tcacgtaatc ggctccgata tttcttgcca
    23161 gattttgtct atatggagtt atttcaatgg caatgaccaa tttcgctccg agtgctttgg
    23221 caacgtccac tcccatgatt ccgatcggtc cgcaaccgac gatggcgact gttttatcct
    23281 tgaccggaaa gtgagcgatc gtatgaactg cattccccaa aggctcctgg actgacagga
    23341 aaagcatgtt ctgtccggga tcgttaatga agcaattggc atccggaatt ttcacataac
    23401 gggcaaaaca tccatcggta tcgacaccga ttattttcgt gtttttgcat atgtggcctt
    23461 ccccgcgttt gcagaattcg cattctccgc agattatgtg ggtttctgca ctgactatgt
    23521 ctcctacctt tactctcttt acatccgttc cgattttgat gatttcacca ctgaattcat
    23581 gtccagcagt caaaggtaat ttgagtcgtt tttgcgccca ggcattccaa tcgtaaatgt
    23641 ggtagtccgt gccgcaaaat gatgttgtca gtactttaat tagtacttcg tgcggtccca
    23701 gtttagtgga aatctttttc tgcgtgtaag tgaatcccct ttcgggtttg tccttgacaa
    23761 cggccatcat ggtcagttca tcaggcaatt cctgacttgt cgtcataatc ttcatatgct
    23821 ccttttctat tgttttacaa acgctttcat cataataaat tataccacaa aggcttatgt
    23881 tccgaatggt ttttttttgg aaaacacatg actttttttc attagctttc atcacaaacc
    23941 ggaaaacggt ttcagataat catttatgtt gatattcaaa gaaatatatt catccaaaaa
    24001 tgataattat gttataatta tttatggtga ttacagatga ataaggcaaa tttgttcatg
    24061 aaaatggaat ttttgaccaa gtactccgat cttccggcaa caattgtcga tgatgacaat
    24121 tccatacttt ggtgctcttt caatcagatc gattttgacg cgcttcccat ttatccgtat
    24181 gtaaaggatc ttgaaataaa agaaggcctg ccaaaattgc ttattttcaa ccggatcgaa
    24241 attcacggtc tttttagaat cggatcatat cgcttcctca tcggaccggc aatcaccgcc
    24301 aaaccactaa cagaaatcca ttatgcgttg ctatcagcat ccaattgcag tcaggaaatc
    24361 agcctcgaca caatcagaaa cattcctttc gtgtccatct gcaagttcat tcaatatctc
    24421 tctttgcttt gcttcatcat cgacggaaaa agatttagaa ccgaagatat tttgcaaaac
    24481 aagatcaatg tcgatactcc cgctcttgac aatttacgtt ccgattttct tcccgagagt
    24541 accgacaggt tcggattgat tccccagtcc aatcccgatc taagagaaat ctccgaaata
    24601 tataattcca taaaagccgg gaatgaggaa gaagtcgcaa gattaatcat tgattcctct
    24661 ctagcagcca aattcaaaat cgctagcaag aaagaaaaca gcgcgattct cggcgctgca
    24721 gcaactcttc ttgccaaagc ggcatttgac gccggtgtcg gtttcgacga tattt
//
LOCUS       DAOPJL010000019        24104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_2214_length_24104_cov_8.910474, whole genome shotgun sequence.
ACCESSION   DAOPJL010000019 DAOPJL010000000
VERSION     DAOPJL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24104
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2214_length_24104_cov_8.910474"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(269..348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            560..3910
                     /locus_tag="PLP02_03660"
     CDS             560..3910
                     /locus_tag="PLP02_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42579.1"
                     /translation="MESTMTKRIIIIFSVLITVVLAAVVLTFSTGNTHSPAVDNPNDI
                     FYEKLDGDGNVIYTITNEEMFESIKKTDGLQQLLFMIDEDLLANYFNQITEDELQDEI
                     NNEIYGTSDADEIAAMDADTKTALEESYDQNMILAGFDGNEEAYATIVLVRQAYVRDA
                     IVENDDVSDVDIAKDFINNTFDDIKAIRIRFMSATDASTVLQHFNLLSLNSKTLRDYN
                     GYTFKLNSLFDENDEIVQAYQTVTPYYYTEDGDIANLADDVIYTFGGNGIYVDEDDAE
                     YTLDASGNLIDADQEIAVAYSLLFDSSDLAEAYKQANTHYYTVSKTDPSDEEESTKVY
                     DRDGEQLAFTIDPDGTIYDQENNDVTNTTSLYVNKYYTSIENVSSASVYNSTELSNEE
                     VLAKYIEIYNYVYGDYRSLLAEDATAEDLIALDNAYLTFNFDAINAVNSDLATYMFET
                     MDISDEDAIPYSGAAQDFNGDEDTYYFMVYKLTQSDKLDAESIMLDNIEANIVIPTTI
                     GEDVTLPATGWYGSSITWVSGDTDYITSDGTVTNPEEDTLVTMTYIVAFNGTTRTGKI
                     DVTILANGETSDITENTDETSFQSILNNDELYYSIQSDLVEAILSASDASDTVTEYLA
                     QMRAEYGFTIEDFYLGLSYAQVYDSYDYDVKGDKKILAYTTGVVGNPDEGVEITAQDF
                     FVYCLNKNAAVYAVYATQYKEALYSTYFEQLFGTEKDLYKNKSDMMETLLTNVESVKA
                     TYANYSTILQAYYGYNSFQEFLYLAYDGAKSESELLENYVLQTVQAFFIKEMVDNYDI
                     LELLYPTVQDYYENYFSLDVNHLLLFFDFDEDGTYDDYFEYRDSLDTIAADNLDTLLS
                     GFKAAVVNYFDEDSDNDLDTLVTEYRSASYDDEVWGDYKKAGFQIMTELLDMTNDDDE
                     EESLTYSGEYGVLDNNYVTEFVDALTALYQEYILPQNEDEDQMFSDLVSTMFGIHIIL
                     ATQGSGFDQPTAIFQESDPLNPVYTSGVENADGEPTLEQFTLYADYYFYSQLFDLANE
                     DVETEYGIVVPEIPATLRTNLDVYTGDLISGLYTVGNINIDIIDRIVTGTFVDNPSNP
                     FTDQEIKLQLVKVQNIYYQALYSEYITD"
     gene            4018..4905
                     /locus_tag="PLP02_03665"
     CDS             4018..4905
                     /locus_tag="PLP02_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPF42580.1"
                     /translation="MKPLKILVLGGTSVDEIVMLDKLPDPYPQTVFGKSFQMVGSTGT
                     GKAVALKRLGHDVCLHTMLGDDEEARIQKEYFRRNGIDLVFGHNAFGTEKHINLMTKS
                     GDRLSIFTRFGYDNKELDKEEVMSLVDKADVICLNIISYLSDMAATIKKAGKPVWVDL
                     HDYDGKNPYYEPFIKSADYIIMSNDALKDPVPVLKQFVRDGKLLAIATKGRDGSIVVD
                     QEKNIHEYGIFDFPYVDSNGAGDSFTAGFITSFYEDGDIEKAQVMATIVAGLAVASSD
                     IAGCNMTWDYVKSYYDNNQ"
     gene            complement(4945..5877)
                     /locus_tag="PLP02_03670"
     CDS             complement(4945..5877)
                     /locus_tag="PLP02_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF42581.1"
                     /translation="MNFEIGQKYDFYGKVEQTNNSTYDTYACNILSEDGENLVVRVNK
                     ETTLSLNKIYFFETEAVVFKEKIHLQASSYRSLSQMDMTDDEKERLFRIFYQYAPINL
                     VQVKKGIEDRLNAIKNKAIREITAEIYNRFADDFYLYPAATRYHHAYISGLSYHTYSM
                     IRLAEGFLSTYPFLNEDLVYAGIILHDVGKILEFDSFEGSEYTIKGRLVGHITMGVEL
                     IRLAAEKLGYMEAEETMLLEHIVLSHHYYGNFGSPKKPNIAEALVIHFLDDADSKLCV
                     LGEELDLVDEGELTSSIGVLDRERYYKHKLTEDK"
     gene            complement(5885..7984)
                     /gene="pulA"
                     /locus_tag="PLP02_03675"
     CDS             complement(5885..7984)
                     /gene="pulA"
                     /locus_tag="PLP02_03675"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HPF42582.1"
                     /translation="MTKDDRKFYCYLDDFDLLTIIVPGANYKPTDTYSLNGNDETIPL
                     VIGQQIALDKETKLVCSFDAYIELRKIYYVENQDGESSELYTGKIVRTELFDNIYRYR
                     KNDLGFTFTKESTKFKIWSPVAKSVRLELLYPNGRKSFHDMEYKNAGVWRHVVDEDLE
                     KVAYRYHVYVNGKETIVEDPYALSSKTHGSYSYVIDKTRLTPIEPLRSFSGNPLDAVV
                     YEMNVRDFSMDDTVPFRHRGKFLGVVEPDLKTPEGHPAGLDHLKYLGITHIQLMPIFD
                     FGDVDETKPDRLYNWGYNPEQYFVPQGWYSTDPDDPYCRIEELRKMIDALHRENIGVI
                     MDVVYNHVFDAKKFPFEKLVPGYAYRVDRQGIYTDVSGCGNDLDTNREMVRKIIIDSV
                     LYWANEFKVDGFRFDLMGLIDFETMNEVRQELSDIDDHFLVYGEGWKMYSTNVGDRMA
                     HMYNKNVLYTIGFFNSIFRDTVKGSTFDILEKGYATGDPVNAETVRQLLFGSALGRYI
                     FKYTTQSINYVECHDNMTFFDKAMKIDKDSVLAKEQEKLATSMVILAQGVPFLHSGQE
                     FYRSKKNVENSFKSGDGINKLDWSLLDENHADVEYLRHLINIRRHYPCFRLKADSELR
                     RSAEAFVLPSKSIMVHYNGECNLIIVFKPLADEEFVVIPEEYRLLLSSVGKKDEPEDG
                     ARLFKNIGTWIFTKESE"
     gene            complement(7981..9168)
                     /locus_tag="PLP02_03680"
     CDS             complement(7981..9168)
                     /locus_tag="PLP02_03680"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPF42583.1"
                     /translation="MEKRFEAARKILAKLIENNHAAYMVGGFVRDRMLGLETDDIDIT
                     TDARPETVELLFESVKETGIRFGTVTVLIDGYSFEVTTFRSEGKYSNFRHPDTIAYSD
                     TLEEDLKRRDFTINAFAMAYDGSVIDIFSGRDDLKRRLIRAIGYPEARFYEDALRILR
                     AFRFVSKLDFQIETGTFKAIRQNRMLLNKIASERILQEMKKIILYPHHAKAFRLMKEA
                     SIGEALPFMNKAIEYLSGREDWRMSDIETYALATFLNQEKVPDEWRFSNKEKEHIHKL
                     AQIVKKTFVAGFDPYTVYEYGFHASIEANNIIKTMDPDKNGEKDIIAFWQKLPIRNET
                     DLAFRGKDILAIPELKDPKQIGKILETIIREIISGRLKNEREAIRGFVRKYMEKELTE
                     KTS"
     gene            9261..9872
                     /locus_tag="PLP02_03685"
     CDS             9261..9872
                     /locus_tag="PLP02_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPF42584.1"
                     /translation="MKTPKYPVTAEHVVNRSRFIGFLSRAESEIEASAFLDKIRADYP
                     DATHHVYAYVLGDNKETQKASDDGEPVRTAGFPVLDVLNKNDVTDAIIVVVRYFGGIK
                     LGSGGLIRAYSATTRKLLEIAEYAYKTPIEDYILHIGYAYTKPVERYLNSHAICIKTE
                     YGEKITYRFSLANGKSDEITALFADMTNGEATLETAATRNVYL"
     gene            complement(9884..10105)
                     /locus_tag="PLP02_03690"
     CDS             complement(9884..10105)
                     /locus_tag="PLP02_03690"
                     /inference="COORDINATES: protein motif:HMM:NF018335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HPF42585.1"
                     /translation="MLDPWVFVITRIVVFFVATAFIFNGLKAVNFEKIFRPNSSGQIR
                     LLIMVLSIVLGYLFADTFVSLFESVNNQL"
     gene            complement(10113..10622)
                     /locus_tag="PLP02_03695"
     CDS             complement(10113..10622)
                     /locus_tag="PLP02_03695"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPF42586.1"
                     /translation="MGDIRKYDWILIGSILLTGVLLFVGMWVWQTIRATGSTYAQVYY
                     KDELVLMIDLDTCEYKIYDTEYKDMIDASRADEGIFYVPGTVTTDMQSLYQDDEYART
                     RQIVGIKLLVYEGKIEVAYQESPRDICELQRPTDSSLEPLVCLPNELVIQIMTDIPSG
                     EFVPDSILE"
     gene            10715..11860
                     /locus_tag="PLP02_03700"
     CDS             10715..11860
                     /locus_tag="PLP02_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPF42587.1"
                     /translation="MKKKLLALMLLLPFIVLSGCDGTRFTNPSPDECTWNDEAEVYVC
                     EKIWIGYFDAPISLKLYYGRDDAVDLEKLYDEVEAMLSEYHEYFDRYNSYNNIVNVYS
                     INQSGTSGIVVSETLFDAIAFGLGHENDITVNGALLFDIALGPVLSIWHDARESDSCV
                     VGSFASTCDVPSELIDGISFNTDPSDIDMDRDNLTITFLKPNMSIDLGGYGKGYVSEI
                     ITDYLDSLDLDYIFNAGESNLKAGGTNPTRDNGVFYIGLKKPEVDALSVDFYAYLKIT
                     AGISVVTSGNYQRYFLGSEDGEIYHHIIDPRTNYPGGEAMSVTIFYEDGALADIYSTA
                     IFLMTVEEGITYVDETEGLEAIWYLEDGTIMTSANFTDIYLYQYPDS"
     gene            complement(11887..12402)
                     /locus_tag="PLP02_03705"
     CDS             complement(11887..12402)
                     /locus_tag="PLP02_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPF42588.1"
                     /translation="MKTRKLVFLAVMAAVAVALSIVESVISTFIFIIPGVKLGLANII
                     TLVIMYYYSDRDAFLVVVIRIFLTGLLYTGLFEPAFWMSLAGGLLAFLAMFILKRVKI
                     FSIMSVSVFGALMHMVGQILAAIVVLDTETLLYYLPYMMVIAVPTGIFTALVAKKLLV
                     ILKKQASTTTK"
     gene            complement(12476..13180)
                     /locus_tag="PLP02_03710"
     CDS             complement(12476..13180)
                     /locus_tag="PLP02_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPF42589.1"
                     /translation="MLEKDPTLFPRTAMASTGTMLEAYEKLRDEGCDEAIFITISSKM
                     SGIYENALLAAKMVEGIKVTVFDSLSVGYVECKMAFTAHDLANQGKSMDEIVKELEFI
                     RDNNHIYFAVSDLKYLVKNGRLSNAAGFMADVLKIKPLLEIDKTGAVVTVEKIRTFQK
                     ALNRVLEKFLEETEGKGYEPFIIHANNPEAVAFLRENVMRLRPEVKEIKDYPLTPAVG
                     AHCGPKALTLGYILKK"
     gene            complement(13190..13330)
                     /locus_tag="PLP02_03715"
     CDS             complement(13190..13330)
                     /locus_tag="PLP02_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42590.1"
                     /translation="MKKLGVLVCTNAALDYIKHDYDIRVIRSTIIMGGKEYVDYEELT
                     AE"
     gene            complement(13435..13875)
                     /locus_tag="PLP02_03720"
     CDS             complement(13435..13875)
                     /locus_tag="PLP02_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HPF42591.1"
                     /translation="MKTTSGRIEVIAVSACLLGIASTYRGDNNLSLHTIEKIKGKIVF
                     PVCPEAMGGLPTPRIPSEIQNDGITVLAKDKTDVTAFFQRGASIALDIAVLNGCHKAI
                     MKENSPSCGSGYVYDGTFSGKLIKGKGYACRIFQKSGIDVENEK"
     gene            complement(13872..14123)
                     /locus_tag="PLP02_03725"
     CDS             complement(13872..14123)
                     /locus_tag="PLP02_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPF42592.1"
                     /translation="MAVNFEKLIVDLVSPLVVHPEDLMVKKFSEDETTITIQVLVNKE
                     DLGRVIGKGGKIANAIRTISYAAASRLGKRVSIDIDSYE"
     gene            complement(14136..14405)
                     /gene="rpsP"
                     /locus_tag="PLP02_03730"
     CDS             complement(14136..14405)
                     /gene="rpsP"
                     /locus_tag="PLP02_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPF42593.1"
                     /translation="MVKLRLQRFGTTKRPFYRIVAAEARKKRDGRYLEIIGQYDPTKE
                     PVLINIDAEKALKWLSEGAQPTDTVKNLFTKTGITKQFIESKKSK"
     gene            complement(14493..14723)
                     /locus_tag="PLP02_03735"
     CDS             complement(14493..14723)
                     /locus_tag="PLP02_03735"
                     /inference="COORDINATES: protein motif:HMM:NF021426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HPF42594.1"
                     /translation="MVNRKGIIVYFQSKKIVKEVEKTGVNVVYINPKAKYLTGYIDAS
                     EYEKARKQIQKIRLVRKVEESLVDMEEFSFNE"
     gene            complement(14790..15509)
                     /locus_tag="PLP02_03740"
     CDS             complement(14790..15509)
                     /locus_tag="PLP02_03740"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPF42595.1"
                     /translation="MIRLDRFLSELKYGSRSMIPGLVRNKRVMVDGKLAESVNMKIDP
                     EKDRVMVDGIEVFFRRSLVLMLYKPKMTESSNMDGAHPSVLGLIGEPYSRLDLNIAGR
                     LDADAEGLMLLTNDGTLLHRIISPNNHVEKTYLVKLADPIGDYSPLLAGVTIQDGKNQ
                     PFLTSPARIEVIDKYSCKITISEGKYHQVKRMFAYLGNQVTDLKRISIGKLSLDTSLA
                     PGSYRELTEAEIAAIFAEKTV"
     gene            complement(15506..17473)
                     /locus_tag="PLP02_03745"
     CDS             complement(15506..17473)
                     /locus_tag="PLP02_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPF42596.1"
                     /translation="MWYSLDSETIRKKEEMSRLEWSLSNGLGGYASGPVSGQLFRKHH
                     GLLVASLEPPVRRVMAISKIIEEIHTDSGTVSLEEISAKTSGPSSGLVEFCYERTVKW
                     KYRLGNLILTKEIAPLYGKNAVAINYRVYSPEIVKLVLTPWFAFREHSQVENMPFAHM
                     TYEKEDNKAKVYSRLYPGHVTILSFSQGEILPTDKQDARIGEYDLDRYNGDDRSDCFF
                     TPCKLVVTIPADKETEIGLVGSMDETIEFTANEIIRKYSAKIDGILNAAKSCTTFEKR
                     LWVSAEQFIAKKAETNATTILAGLPWFSDWGRDTMIALPGLLLSTGKFKMAKEVLLSF
                     SRYLKNGLIPNVFPNSGAKPLYNTVDASLWYIIAIYRYFAATGDLAIIKRRFYPDIAE
                     ILAWYEKGTDFHIRMDSDGLISAGSGSDQITWMDVKIDDKPITPRQGKPVEINALWYN
                     SLMIAVKFSSLLGIDGDKYLLMAETAKNSFVKRFWNQKTGCLFDVVDPDDPSIRPNQL
                     YAISLPFDIMPKKKAISIWELAKQELEDIYGIRTLSLYNDNFKPEYSGSIDKRDRAYH
                     MGTSWGFLFGVYLEACLRVNDFSTDSREYVRHCFLRLEKELEEGCLNGYAEIFDGQNG
                     EIGKGCYNQAWSVGEILRIYHDYGFGKEVTK"
     gene            17634..18209
                     /gene="def"
                     /locus_tag="PLP02_03750"
     CDS             17634..18209
                     /gene="def"
                     /locus_tag="PLP02_03750"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013877258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPF42597.1"
                     /translation="MIMMKDIIQEGDPILRKRSENVPLPLNNKDLETLRQMMEYIVNS
                     QDEEISKQYGLRPAVGLSAPQIGLNRKLFCMRCPDEKGIAMHSYAVCNPTIVSFSEEQ
                     TYLGGGEGCLSVPENENGLVPRSMRIKARMVLVDLETGNMEKVLMKLSGYPAIVFQHE
                     YDHLLGILFVDKVKPELPKIKPIAFVSDEAE"
     gene            complement(18234..19205)
                     /locus_tag="PLP02_03755"
     CDS             complement(18234..19205)
                     /locus_tag="PLP02_03755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPF42598.1"
                     /translation="MGKCISIGEVLVDFIPDAGNWKLKDVPIFRRQAGGAPANVAAAV
                     ARLGGRSVVLSKLGNDPFGDFLIDEMKKTGIDTSYIKHTDEASTGLAFVSLKPDGNRD
                     FCFYRNPSADMLLSQDEIDLDIFEEGDILHFCSVDLVDFPVRKAHEKAIGIAKEKRLI
                     VSFDPNIRLSLWENKEELREIVRRFIPLSDILKISEDELEFITGKKDITGALSSLFIG
                     DVKVIVYTMGANGSAVYTKDRTFVHDGFVVNAIDTTGCGDAFIGAFLYMILSGSLTKE
                     SLGKSDYQKILEFSNAVGSLVATKKGALASMPGYMETEMFLKEKKAK"
     gene            complement(19229..20440)
                     /locus_tag="PLP02_03760"
     CDS             complement(19229..20440)
                     /locus_tag="PLP02_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPF42599.1"
                     /translation="MQKNTYKLKKGIYWVGVNDYELRVFDIIMRTEFGSSYNAYLVKG
                     NDKVALIDTVKANFSADYLAKVGSLVDFSAIDYLIVNHTEPDHSGSIADILDKNPDIT
                     IVGSAPSLSNLKEILNRPFKSLRATPDLTIDLGNKTLKFMIRPNLHWPDTIFTYVPED
                     GILFTCDFFGAHYAFEGVLAKNVPDRKDFAKAFKEYFDAIMSPFRPFVFKGIEGLEEL
                     NLEYIATSHGAVLDETFIKEALHKYAEWAKKPEPREKPFVVIAFASAYGYTARMAEVI
                     AKAINFEFGGKIDIETYDVVTTDIETIVGRIGESDGFLIGTTTILRDAVYPIWDMLSR
                     LNPETDGGKTAGAFGSYGWSGEAVQNVTERLKQLKMKTVDGLRIRFKPSDSQIQTAYD
                     FGIGFAKKLKE"
     gene            complement(20568..21278)
                     /locus_tag="PLP02_03765"
     CDS             complement(20568..21278)
                     /locus_tag="PLP02_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPF42600.1"
                     /translation="MRLGIISDIHNNVIALETMLEEFAKRQCDGLICAGDIIGIGPYP
                     EETVSRFMKIPNLFACVAGNHERYFAEGIIPVREMSDVEKEHHVWEHAFLSEESGNYL
                     KSLPCETYLNIAGKRLYIAHYAMNGESRYMDISDEASSSDMDRIFSNIDADIVIFGHD
                     HKGFVANGKALYLNPGSLGCPGRNLDKTRGIVLDIGKETTWEQLSITYDVQKVTDDIR
                     LLEYPAMDEILRYFYGIR"
     gene            complement(21302..21607)
                     /locus_tag="PLP02_03770"
     CDS             complement(21302..21607)
                     /locus_tag="PLP02_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42601.1"
                     /translation="MKLHFIRLKSPRFYLVPMDIYVDNEFRNDLRNNQEIELDCNQKP
                     KLMISGSIFTKNLEIDLSSLEDETVTIYLEYIFGWWMNGIRAFIVGNDNRFIGTYTT"
     gene            complement(21609..22220)
                     /locus_tag="PLP02_03775"
     CDS             complement(21609..22220)
                     /locus_tag="PLP02_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPF42602.1"
                     /translation="MQKGYRHRYSLWEVLIVFSQSMRAFPIMAASRLKHRIDPHFQER
                     LMLAVTEVNGCEVCSYAHTQVALKQGMDSAEIASFLQGDGEMIKETEAKAIIFAQHYA
                     DSNGRPEHYAYDVLLEEYGKKKTKAILATIQIMMMGNVFGLPMSAFFSRLKKQPYHNS
                     NIFYELVLLIAPMYLFPFALLTALCLWPIVPGNIIFAKESGGV"
     gene            complement(22312..22560)
                     /locus_tag="PLP02_03780"
     CDS             complement(22312..22560)
                     /locus_tag="PLP02_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015240072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPF42603.1"
                     /translation="MTYGSIASYAGNRYGARQVVRILNSSSISYNLPWHRVINSKGEI
                     GLSGEGGAMQRQKLEAEGVEFKDGKIDLSKYSHKFVLE"
     gene            22896..23732
                     /locus_tag="PLP02_03785"
     CDS             22896..23732
                     /locus_tag="PLP02_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42604.1"
                     /translation="MDKRGLVLSVLGLSVSFLTLVIATFAWFAISTTVDNSMLGMNVG
                     AGIFPSYDIHYYTYDHVYKYDQSSESILVYDGGFWVAPSYEVLPESAYAFQGIMIGSY
                     DPLVPINNEYDNIIIELHLYCDADESMSFSLGASADTSMAAGAVLTFGDAGTIYYLSQ
                     IAYLQEMETDNYQANPEGTNIYSSLKSDFETVDGNEDLIYPLHGFYDEYDAYVSETSL
                     GSLSVTSATTDVYLYFNISYNTAKIDTILSGEGSGSTITSVVALRFYQDIKIYIEEAS
                     GS"
     gene            23729..>24104
                     /locus_tag="PLP02_03790"
     CDS             23729..>24104
                     /locus_tag="PLP02_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42605.1"
                     /translation="MTRKKRNLLPIVSLLVALLFVVSLAVTTYGWIVNYYSYSQDVLG
                     KSGALYFADGDGSSSDPFIISEPNHFYNLAYLQNIGAFGSTTYYFKVADPDTGLAITI
                     DFSDPSYLSTYQTVYPIGADPDA"
BASE COUNT         6352 a         5732 c         5264 g         6756 t
ORIGIN      
        1 gtctggtatt ttcccgtaaa ccaggcatag gttgcgaaaa caaacatgca aagtgagaat
       61 attgccagga taaacgacaa aaaaacttgt cttttcaggc tggctgtatt catatccgat
      121 ccctcgcttt ccgtaaatgt caattagaaa aagaaaatac tttgagcgcg tctttttaga
      181 aaaaacgcca ttgtccatcg acggcatttt tttatttcaa aggataattc agccctggaa
      241 ataaaaaaac gccctcgtct ttacagacaa cggcaactgg ctaccatctt ttacgaaggc
      301 cctatagctt tgcgtcacta gctttcgcta gttttgctat ttacggcgtt attatataat
      361 ttactgtttt atttgtcaat aaacatcttc atttttttat gaaattcaaa caaataatct
      421 accgcctctg aaacatccca tttggcggga aagaccatgt caggctcctt ctcatgtatt
      481 tatcacaata tttttattgt ttttcaaatt gaataatggt atgataaata gtgcgaattt
      541 aaccaaggag cgtaccacaa tggaaagtac aatgaccaaa agaatcatca tcatcttttc
      601 cgttttgatc acagttgtgc tggctgccgt cgtcctgact ttttcgacgg gtaacaccca
      661 ctcgccggct gttgacaatc ctaatgatat cttctacgag aaattggacg gtgacggaaa
      721 cgtcatctac acaatcacca acgaagagat gttcgagtcg atcaagaaga ccgacggtct
      781 tcagcaactt ctgttcatga tcgatgagga cctgttggca aattacttca accagatcac
      841 tgaggatgaa cttcaagacg agatcaacaa cgaaatatac gggacaagcg atgcggatga
      901 aatcgccgct atggatgccg acaccaaaac cgctttggaa gagagttacg accagaacat
      961 gattctggcc ggattcgacg gcaacgagga agcttacgcg acgatcgttc tcgtacgcca
     1021 agcttatgtc cgcgacgcca tcgttgagaa cgacgacgtc agtgatgtcg acatcgccaa
     1081 ggatttcatc aacaacacct ttgacgacat caaagccatc cgtatccgtt tcatgagcgc
     1141 taccgatgcg tcgacagtgc ttcagcattt caatcttctc tcgctgaact cgaagactct
     1201 ccgcgattac aacggctaca ctttcaaact caactcgctg ttcgatgaga acgacgaaat
     1261 cgtacaggct tatcagaccg ttaccccgta ttattacacg gaagacggcg atatcgccaa
     1321 tcttgccgac gatgtgatct acaccttcgg cggaaacggt atctatgtgg acgaagacga
     1381 cgccgaatac acgctggacg ccagcggcaa tctgatcgat gccgatcagg agatcgcggt
     1441 ggcatacagt ctgttgttcg attccagcga tctcgccgag gcttacaaac aggccaatac
     1501 gcactattac acggtttcca aaaccgatcc ctccgatgaa gaggaatcca ccaaggttta
     1561 cgaccgtgac ggcgaacagt tggctttcac gatcgatcca gacggaacca tctacgacca
     1621 agaaaataac gatgtcacga atacaacttc gctttatgtc aacaaatatt acacttcgat
     1681 cgaaaatgtt tcttctgctt ccgtatacaa ctcaacggaa ctttccaatg aagaagtcct
     1741 tgccaaatac atcgaaatct acaattacgt ttacggggat taccggagtc tgcttgctga
     1801 ggatgccaca gccgaagacc tcatagcgct tgacaacgct tacctcacct tcaacttcga
     1861 cgcaataaac gccgtcaaca gcgatctcgc cacatacatg ttcgagacca tggacatttc
     1921 cgatgaagat gccatcccgt attccggcgc cgcccaggat tttaacggcg acgaggacac
     1981 atattacttc atggtataca aactgacaca atccgacaaa ctcgatgccg aaagcatcat
     2041 gctcgacaac atcgaagcga acatcgtcat cccgacaacg atcggcgaag acgtcacgct
     2101 tcccgccacc ggctggtacg gatcgtcgat cacttgggtt tccggcgaca ccgactacat
     2161 cacgtcagac ggaaccgtta ccaatccgga agaagacaca ctcgtcacca tgacctatat
     2221 cgtggccttc aacggaacca ctcgcaccgg taaaattgat gtaacgatcc tggcaaacgg
     2281 cgaaacatcg gatataacag aaaacaccga cgaaacttcg ttccaaagca ttctcaataa
     2341 cgatgagctg tactattcga tccaaagcga cctggtggaa gctatcctgt ccgcttctga
     2401 cgccagcgac accgttaccg aatatctggc acaaatgcgg gccgaatacg gtttcacgat
     2461 cgaagacttc tatctcggtt tgtcctatgc ccaggtatat gacagttacg attacgacgt
     2521 caaaggcgac aagaaaattc tcgcctacac gaccggagtc gtcggtaacc cggacgaagg
     2581 cgtcgaaata accgctcagg atttcttcgt atattgcctt aacaagaacg cggctgttta
     2641 cgccgtatac gcgacacaat ataaagaagc tctctattcc acgtatttcg aacaactgtt
     2701 cggaacggaa aaagatcttt acaaaaacaa aagcgatatg atggaaacct tgctaacaaa
     2761 tgtcgaatca gtcaaagcca catacgccaa ctattcaacc attcttcaag cttactacgg
     2821 atacaattct ttccaggaat tcctctacct tgcctatgac ggcgccaaga gcgaatccga
     2881 attgcttgag aattatgtcc ttcagaccgt ccaggcattc ttcatcaaag agatggtcga
     2941 caactacgat attctggaac tgctctatcc gaccgttcag gactattatg aaaattactt
     3001 cagcctcgat gtcaatcact tgcttctctt cttcgatttc gacgaagacg gaacttacga
     3061 tgattacttc gaataccgcg acagcctcga tacaatcgct gccgataatc ttgacacttt
     3121 gctttccgga tttaaagcgg ctgtagtcaa ctacttcgac gaggattccg acaatgatct
     3181 ggatacgcta gtcaccgagt atcgctcggc atcctatgat gacgaggtct ggggcgatta
     3241 caagaaagcc ggattccaga tcatgaccga actgcttgac atgaccaatg acgacgacga
     3301 ggaagaatcc ctgacttatt cgggcgaata cggcgttctg gataataatt atgtcaccga
     3361 attcgtcgat gcccttactg ccctgtatca ggaatacatc ttgccacaaa acgaggatga
     3421 ggatcagatg ttctccgatc tcgtctcgac gatgttcggt atccacataa tccttgcgac
     3481 ccagggaagc ggctttgatc aaccgaccgc cattttccaa gaaagcgatc cgctcaatcc
     3541 ggtttacaca agcggagtgg aaaacgcaga tggcgaaccc acattggaac agtttacgct
     3601 atatgccgat tattatttct acagccaact cttcgatctt gcaaacgaag acgttgaaac
     3661 ggaatacggc attgtcgttc ccgaaattcc tgcgacgcta agaacgaacc tcgatgtata
     3721 cacaggagat ctgatttccg gactatacac cgtcggcaac atcaacatcg acatcatcga
     3781 ccgcatcgtg accggtacgt tcgttgacaa tccaagcaat cctttcaccg atcaggaaat
     3841 caaactccaa ttggtcaaag tccaaaacat ctactaccaa gctttatact ccgaatacat
     3901 caccgactaa tcatttgaca aaataaagcg gatccgaaaa acgatccgct tttttgtttc
     3961 cctaaaaacg aaaatgattt ataattgctc ttgaaggcac atattcggag gaaaacgatg
     4021 aaacctttga aaatcttagt cctcggcggc acttccgtcg acgagatcgt catgctggac
     4081 aaacttcccg atccttatcc tcaaaccgtt tttggaaaat catttcaaat ggtcggaagc
     4141 accgggacgg gcaaagccgt cgctttgaaa cggctcggac acgatgtttg cttgcataca
     4201 atgcttggcg acgatgaaga agcccgaatc cagaaagaat acttccgccg aaacggaatc
     4261 gatcttgttt ttggccataa cgcctttgga acggaaaagc atatcaacct gatgacaaaa
     4321 tccggcgacc gtctcagcat tttcactcgc tttggttatg acaataaaga attggacaaa
     4381 gaagaggtca tgtcgcttgt agacaaggcg gacgtcattt gtctcaatat catttcttat
     4441 ctaagcgaca tggcggctac aatcaaaaaa gccggcaagc cggtttgggt cgatctccac
     4501 gattatgacg gcaaaaaccc ctattatgaa cctttcatca agtcggccga ctacataatc
     4561 atgagcaatg atgcattaaa agatccggta cccgttctca aacaattcgt ccgcgatggc
     4621 aaattactgg caatagctac caagggccgg gacggttcta tcgtcgttga tcaagagaaa
     4681 aatatccatg aatacggaat tttcgatttt ccgtatgtcg actcaaacgg ggcgggcgac
     4741 agttttactg ccgggttcat aacatctttt tatgaagatg gcgacatcga aaaagcccag
     4801 gtgatggcaa cgatcgttgc cggtctcgca gtggcttcaa gcgatatcgc cggatgcaac
     4861 atgacatggg attatgttaa aagctattat gataacaatc aataatttca gaaaagaaaa
     4921 gcgtcgcgga tttccgcgac gttttcattt gtcttccgtc agtttgtgtt tgtaatacct
     4981 ctcccggtcc aaaaccccga tcgaggaagt aagttcgccc tcatcgacca gatcaagttc
     5041 ctcgccaagc acgcaaagtt tggaatcggc gtcgtcgagg aaatgaatga cgagcgcttc
     5101 ggcgatattc ggtttcttag gtgatccgaa gtttccgtaa taatggtggc tcagtacgat
     5161 atgctccaga agcattgttt cctccgcttc catgtatccg agtttttcag cggcgagccg
     5221 gattaattcc actcccatcg tgatatgtcc gacgaggcgg cctttgatcg tatattcgct
     5281 gccttcgaac gaatcgaact cgagaatttt tccgacatcg tgcaaaatga taccggcata
     5341 aacaagatcc tcattcagaa acggataggt cgacaggaac ccttccgcca gccggatcat
     5401 cgaatacgtg tgatatgaaa gtccggatat gtaagcgtga tggtaacggg tggcggcagg
     5461 ataaagatag aaatcatccg caaaccggtt gtaaatctcc gcggttattt ctctgatagc
     5521 tttgttcttt atggcattga gcctgtcttc gatccctttt ttcacttgaa ccagattgat
     5581 cggagcatac tggtagaaaa tccggaacag tctttctttc tcgtcatcgg tcatatccat
     5641 ctgggaaagc gaccggtagg aagaagcctg gagatgaatc ttctctttga agacgacggc
     5701 ttctgtttcg aaaaagtaga ttttgttcag tgacagagtg gtttccttgt tcaccctgac
     5761 gacgagattt tctccgtcct cgcttagaat attgcaggcg taggtgtcgt aggtggaatt
     5821 gtttgtctgt tcgacttttc cgtaaaagtc gtatttttgt ccgatttcaa aattcatgtg
     5881 gcgttcactc actttctttc gtaaaaatcc aggttccgat atttttgaag agccttgctc
     5941 cgtcttccgg ttcatccttt ttcccaacag atgacaacag tagacggtat tcttccggta
     6001 ttacgacaaa ttcctcgtct gccagcggtt taaaaacgat gatcaggtta cattctccgt
     6061 tataatggac catgatcgat tttgatggca aaacgaaggc ctccgccgat cttctgagtt
     6121 cggaatcggc tttgagacgg aagcagggat agtgcctacg gatgttgatg agatgtctca
     6181 gatattccac atcggcatgg ttttcatcca gaaggctcca atcgagctta ttgattccat
     6241 cacccgattt gaaagaattc tccacgtttt ttttcgaccg gtagaattcc tgaccggaat
     6301 gcagaaaggg gactccttgg gccaaaatga ccatcgatgt cgccaacttc tcctgctctt
     6361 tcgccaggac ggaatccttg tctattttca tcgctttgtc gaagaacgtc atgttgtcgt
     6421 ggcattccac ataattgatc gactgggtgg tgtatttgaa gatgtaacgc cccagagcgc
     6481 ttccgaaaag caactgtctc acggtttcgg cgtttacggg atcgccggta gcgtagcctt
     6541 tttccaggat gtcgaaggtg ctccccttga ctgtatcccg gaatatgctg ttgaagaatc
     6601 cgatggtata gagtacgttt ttattgtaca tgtgagccat ccggtccccc acgttggtcg
     6661 aatacatctt ccaaccctcg ccgtaaacca gaaaatggtc gtcgatgtcc gacagttcct
     6721 gtctgacttc gttcatcgtc tcgaaatcga taagtcccat cagatcgaaa cggaacccgt
     6781 ctaccttgaa ttcgttcgcc cagtacagca cagagtcgat aatgattttc cgcaccatct
     6841 cccggttggt gtcaagatcg tttccgcacc cagagacatc ggtgtaaata ccttgcctgt
     6901 cgacacgata tgcataaccg ggaaccaatt tttcaaacgg aaatttcttg gcgtcgaaca
     6961 catggttgta cacaacatcc atgatgacgc cgatgttttc ccggtggaga gcatcgatca
     7021 ttttccttaa ttcctcgatc cggcaatacg gatcatccgg atcggtggag taccatccct
     7081 ggggtacgaa atactgttcg ggattgtagc cccagttgta aagccggtcc ggtttcgtct
     7141 cgtcgacatc gccgaaatcg aagatcggca tcaattggat gtgggttatg ccgagatatt
     7201 tcagatgatc gagtccggct ggatggcctt ctggtgtttt cagatccggt tccacgacgc
     7261 caaggaattt cccccgatgc cggaaaggca cagtatcgtc catcgaaaaa tcacggacat
     7321 tcatttcata gacaacggca tccaaaggat tgccagaaaa gctcctaagt ggttcgatcg
     7381 gcgtcagtct ggttttgtca atgacatacg aatagcttcc gtgtgttttg ctggaaaggg
     7441 cgtaaggatc ttcgacgatc gtttccttgc cgttcacata gacatggtaa cgatatgcca
     7501 ccttttccag atcctcgtcg acgacatgac gccagacacc ggcgttttta tattccatat
     7561 catgaaatga tttgcgtccg ttcggataca aaagctccaa acggaccgat ttagccaccg
     7621 ggctccagat cttgaatttc gtcgattcct tcgtgaaggt gaatccgaga tcgttcttgc
     7681 ggtaacggta gatgttgtcg aacagttcgg tccggacgat tttgccggta tatagctcgg
     7741 acgattcgcc gtcctggttt tcaacgtaat agatctttct aagttcgatg taggcgtcga
     7801 acgaacaaac caatttggtt tctttgtcaa gcgctatttg ttgtccgatc acaagcggga
     7861 tggtctcgtc gtttccgttc agactgtatg tgtccgtcgg cttatagttt gcaccgggaa
     7921 cgatgattgt caaaagatcg aaatcatcaa gataacaata aaatttgcgg tcgtcttttg
     7981 tcatgatgtc ttctccgtca gttctttttc catatatttc ctaacgaaac cgcggatggc
     8041 ttcgcgttcg tttttcaagc gtccggaaat gatctcccgg atgatggttt cgagaatttt
     8101 cccgatttgt ttcggatctt tcagttccgg tatcgctaaa atgtcttttc cccggaaagc
     8161 aaggtcggtt tcattgcgga tgggaagctt ctgccaaaaa gcgataatgt ccttttcccc
     8221 gtttttatcc ggatccatcg ttttgatgat gttgttggct tcgattgagg catggaaccc
     8281 gtattcatag actgtatatg gatcgaatcc ggctacaaat gtttttttga cgatttgcgc
     8341 cagtttgtgg atgtgttcct tttccttatt ggaaaagcgc cattcatccg gaactttttc
     8401 ttgattgaga aaagtggcca gggcataggt ttcgatatcg gacatccgcc agtcttcccg
     8461 gccggaaaga tattcgatcg ctttgttcat gaaaggaagg gcttcgccga tacttgcttc
     8521 cttcatcagg cggaatgcct tggcatgatg gggatagaga attattttct tcatttcctg
     8581 caaaatgcgt tcgctggcga ttttattcag aagcatgcga ttctggcgaa tggccttgaa
     8641 cgttccggtt tcgatctgaa aatcgagttt gctgacaaaa cggaaggctc tcaatatccg
     8701 aagcgcgtcc tcatagaatc gcgcttccgg atatccgatc gcacggatga gacgcctttt
     8761 taagtcgtcg cgcccggaaa atatgtcaat caccgacccg tcataggcca tggcgaaagc
     8821 attgatcgtg aaatctcggc gtttcaagtc ctcttccagg gtatcggaat aagcaatcgt
     8881 gtccggatga cgaaagttcg aatattttcc ttcactacgg aatgtggtca cttcgaacga
     8941 atatccatct atcaagactg tgaccgttcc gaatctgatt cccgtctcct tgacgctttc
     9001 aaaaagcaac tcgactgtct ccggcctcgc atcagtcgtg atgtcgatat cgtctgtttc
     9061 gagaccgagc atccgatcgc gaacaaaacc gccgaccata taggcggcgt gattgttttc
     9121 gatcagtttt gccaggatct ttctggccgc ttcgaaacgt ttctccaaac aaatcacctt
     9181 taaataatta tatcatatag atgtccgtta ttctatcaaa aaccggcgac gctgtgttat
     9241 aatgagaccg gtgatgcgaa gtgaaaacgc ccaaatatcc ggtcacggcg gaacatgttg
     9301 tcaaccgttc ccgattcatc ggttttttat cgcgggcaga gtcagaaatt gaagcctccg
     9361 ccttcttaga taaaatccga gcggattatc cggacgcgac acatcatgtc tacgcctatg
     9421 tgctgggaga caataaggaa acccagaaag ccagcgacga cggcgagccg gtaagaaccg
     9481 ccggatttcc ggttctcgac gttttgaaca aaaacgatgt aaccgacgcc attattgtcg
     9541 tcgtcagata ctttggcgga atcaaacttg gttcgggcgg attaatccgc gcttattccg
     9601 ccacaactag aaaacttctg gaaatagctg aatacgcgta taagacgccg atagaagatt
     9661 acatccttca tatcgggtat gcttatacca aaccggttga acgttaccta aacagccatg
     9721 ccatctgtat aaaaactgaa tacggcgaga aaatcacgta ccgtttttcg ctggcaaatg
     9781 gcaaaagcga tgaaatcacc gcgcttttcg ccgatatgac aaacggcgaa gccacattgg
     9841 aaacggctgc gacgagaaat gtttatctct gattcaaaat tcgttataac tgattgttga
     9901 cggattcgaa aagcgacaca aaagtgtcgg cgaacagata tccgagaacg atcgaaagaa
     9961 ccatgatgag aagtcggatc tgcccggaag aattcgggcg gaaaatcttc tcgaagttga
    10021 cggctttcaa accgttgaaa atgaaggctg tcgccacaaa aaagacgaca atccttgtga
    10081 tgacaaatac ccaggggtct aacataaaga aatcactcca atattgagtc ggggacgaat
    10141 tcccccgacg ggatgtcggt cataatctga atgaccagtt cattgggaag acagaccagc
    10201 ggctccaaac tggaatcagt cggtctctgc aattcgcaga tatctctggg gctttcctga
    10261 taagccactt cgatcttgcc ctcatatacc aataatttga ttccgacgat ttgccttgtt
    10321 ctggcatact cgtcgtcctg ataaagactt tgcatatcgg ttgtgacagt accgggaaca
    10381 taaaagattc cttcgtcggc ccggctggca tcgatcatgt ccttgtattc tgtgtcgtat
    10441 atcttgtatt cgcaggtgtc cagatcaatc atgagaacca attcatcctt gtaatagacc
    10501 tgcgcatatg tgctaccagt cgcccggatt gtctgccaaa cccacattcc gacaaaaaga
    10561 aggacgcccg tcaaaagaat cgatccgatc aggatccagt cgtattttct gatgtcgccc
    10621 acctcctctt tatctataaa ttattataca ccaatacggt tgttttacgc cggcgaatgg
    10681 aaaaacatat ctgtattggc cttatggtga tgaaatgaaa aagaaattgc ttgcgctcat
    10741 gttgcttctt ccatttatcg tcctgtccgg atgcgacggg acgcgtttta ccaatccttc
    10801 gccagacgaa tgtacatgga acgacgaagc ggaagtctat gtctgcgaaa agatctggat
    10861 tggctatttc gacgcgccga tctcgctcaa actatattat ggtcgtgatg atgctgttga
    10921 tctggaaaaa ctatacgatg aagtagaagc aatgctttcg gaataccatg aatatttcga
    10981 ccgttacaat tcatataata acatcgttaa cgtctattcc atcaaccaaa gcggaacatc
    11041 cgggatcgtc gtttccgaaa cgttgttcga tgcaattgct ttcggactcg gccatgagaa
    11101 cgacataact gttaacggag cccttctgtt cgacatagcc ctcggccccg tcctttccat
    11161 ctggcatgac gccagagaat ctgacagttg tgtggtcgga tctttcgcga gcacttgcga
    11221 tgttccttcc gaattgattg acggaatctc ttttaacacc gatcctagcg atatcgatat
    11281 ggatcgcgac aatctgacaa tcacgttcct gaaaccgaac atgtcgatcg atctcggcgg
    11341 ttacggaaaa ggctatgtat ccgaaatcat caccgattat ctcgattctt tggacctcga
    11401 ttacattttc aatgccggcg aaagcaactt aaaagccgga ggaacgaatc cgaccagaga
    11461 taacggcgtc ttttatattg gattaaagaa accggaagtc gatgctttgt ctgtcgattt
    11521 ctacgcttat ctgaaaataa cggccggaat atctgtcgtc acaagcggta attatcaaag
    11581 atatttcctt ggtagcgagg acggagaaat ctatcatcac attatcgatc cgagaactaa
    11641 ctatcccggt ggagaagcga tgagcgtgac aatcttttat gaagatggcg ccttggcgga
    11701 tatttactcg acagcaattt ttcttatgac ggtcgaggaa ggaatcacct atgtcgatga
    11761 gacagaaggt cttgaggcaa tctggtatct ggaagacggc acaatcatga catccgcaaa
    11821 cttcacggat atatacttgt accaatatcc cgattcataa aaaaggtgga aacaatttcc
    11881 acctttttat ttggttgtag tggatgcctg ctttttgaga atcactagca gttttttcgc
    11941 cacgagagcg gtgaatattc cggttggcac cgcaatcacc atcatgtacg gcagataata
    12001 aagcaatgtc tcggtgtcga ggacgacgat cgccgccaga atctgcccca ccatatgcat
    12061 aagagcgcca aagacggata cggacatgat cgagaatatt ttgactctct ttaaaataaa
    12121 catcgccaga aacgcgagca acccgccggc caggctcatc cagaacgccg gttcgaatag
    12181 tccggtataa agaagcccgg ttaagaatat ccggatgacg accacgagaa aggcgtcgcg
    12241 gtcgctgtaa taatacatga tgacaagagt tatgatattg gctagtccga gcttgacgcc
    12301 gggaatgatg aatataaaag tagaaatgac ggactcgaca atcgaaagcg cgaccgcgac
    12361 tgcggccatc acagcaagaa aaaccagttt tctcgttttc atctgcacag tccttcctcc
    12421 tggcaagaaa aagacaggcg gcagcctgtc ttttgccagg gttttccggt atcctttatt
    12481 tcttaagaat atatccgagc gtcaaggctt tcggtccgca atgggcaccg actgccggag
    12541 tgagcggata atccttgatt tctttgactt ccgggcggag tctcatgaca ttctcgcgca
    12601 ggaaggcgac ggcctcagga ttgttcgcat ggatgatgaa aggctcgtat cctttgcctt
    12661 ccgtctcttc cagaaacttt tccaaaacac ggttcaaagc tttttggaag gttctgatct
    12721 tctcgacagt cacaacagct ccggtcttgt cgatttccaa aagcggtttt atcttgagaa
    12781 catcggccat gaagccggcc gcgttggata gtcttccgtt tttaaccaga tacttgagat
    12841 cggaaacagc aaaataaatg tggttgttat cgcgaatgaa ttcaagttcc ttgactattt
    12901 catccatcga ttttccctga ttcgcaagat cgtgggcggt gaacgccatc ttgcattcga
    12961 catatccgac cgaaagcgaa tcgaaaacgg taaccttgat gccttcgacc attttggcag
    13021 ccaataaggc gttttcgtag atgccgctca tcttggatga gatcgtgatg aagatcgctt
    13081 cgtcgcatcc ctcatcccgc agcttctcat aggcttccag cattgtgccg gtcgatgcca
    13141 tcgcggtacg cgggaacaaa gtgggatctt tttccagcat gttatagaat tattcggctg
    13201 tcaattcttc ataatcgacg tattcttttc cacccatgat gatcgtcgaa cggataacgc
    13261 ggatgtcata atcgtgcttg atatagtcaa gcgctgcatt cgtacaaacc aaaacgccaa
    13321 gcttcttcat ctgttcatcc cccatttctt catagattaa ttatataatg aaagtagttt
    13381 ttgtcaataa gcgccggtat tatgaaaaaa gaaaaccagt atattctact ggctttattt
    13441 ttcgttttcc acatcgatac cgcttttttg gaaaatacgg caggcatatc ccttgccttt
    13501 gatcagttta ccagagaagg ttccgtcata gacataaccg gatccgcagg aaggcgaatt
    13561 ttctttcatg atcgctttat ggcacccgtt taaaaccgct atgtccaaag cgattgaagc
    13621 gcctctttga aaaaaagcgg tgacgtcggt tttgtctttt gcaagcaccg tgattccgtc
    13681 attctggatt tccgaaggaa tgcgcggagt tggtaatccg cccattgctt ccggacaaac
    13741 gggaaaaacg atctttccct tgattttttc gatcgtatga agggataaat tattatctcc
    13801 gcgataggtc gaagcgatgc ccaacaggca ggcgctaacc gcgatcactt caatcctgcc
    13861 ggatgtagtt ttcattcgta cgaatcgatg tcgatcgaca cccgtttgcc gagtctggaa
    13921 gcggcggcgt aggatatcgt ccggatggca ttggcgattt ttccgccttt gccgatgacc
    13981 cggcccaaat cttctttgtt cacaagaacc tgaatcgtaa tagttgtctc gtcctcggaa
    14041 aatttcttca ccatcagatc ttcgggatga acgaccagcg gagagactag atcaacgatc
    14101 agtttttcga aattgacagc catgtccagc gcgtcctatt tcgacttttt tgattctata
    14161 aactgtttgg ttatgccggt ttttgtgaac aggtttttga cggtatcggt cggttgggca
    14221 ccttccgaca accatttgag agctttttcc gcatcgatat tgattaggac gggctctttg
    14281 gtcggatcgt attgtccgat tatctccaga tatcttccgt ctcttttctt tctcgcttcg
    14341 gcggcgacga tccgatagaa cggacgtttg gttgtaccga atctctgcaa gcgcagtttg
    14401 accattttca tatcctcctg attatttctt tttgtcgaga atattttagc agacaaaaag
    14461 atgcctgtca aggttttttt attgacaggc gttcattcgt tgaaactaaa ttcttccata
    14521 tccaccagtg attcttcgac cttgcggacc aatctgattt tttggatttg tttacgggct
    14581 ttttcgtatt cgctggcatc gatatagccg gtcagatatt ttgccttggg attgatgtag
    14641 acgacgttga ccccggtttt ttctacttcc tttactattt ttttgctttg gaagtagaca
    14701 attattcctt tgcgatttac catatgtttc acctctgaag cgtttaaatc gatatttggt
    14761 tatgaaaatt ataacatgta aatggtctgt cagaccgttt tttctgcaaa aatggccgcg
    14821 atttccgctt ccgtcaattc gcggtagctt cctggggcaa gcgacgtatc cagggaaagc
    14881 ttgccgattg aaatccgttt caggtctgtg acttggtttc caagataggc aaacattctt
    14941 ttgacctgat gatatttgcc ctcgcttatc gttatcttgc aagaatattt gtcaatcact
    15001 tcgatcctgg cgggggaagt tagaaacggc tggttcttcc cgtcttgtat tgtaactccc
    15061 gcaagcaaag gagaataatc gccaatggga tcggcaagtt tgacaagata ggttttctct
    15121 acatggttat tcggagaaat gatccggtgc agaagcgttc cgtcgttcgt aagaagcatc
    15181 agtccttcgg catcggcgtc aagcctcccg gcgatgttca gatccagtct cgaatacggt
    15241 tcgccgatca aaccgagaac ggaaggatgg gccccgtcca tattggacga ttccgtcatt
    15301 tttggtttgt aaagcatcag aacaagggaa cggcggaaaa acacttcgat tccgtcaacc
    15361 atcacccggt ccttttccgg atcgatcttc atattgaccg attcggccaa ttttccatcg
    15421 accatcaccc gtttgttcct gacaagtccg ggaatcatcg aacgcgatcc atatttcaat
    15481 tccgacaaga aacggtcaag tctgatcatt tagttacctc tttcccgaat ccatagtcat
    15541 gataaatccg gagaatttcc ccgacactcc aggcttggtt gtaacaacct ttgccgattt
    15601 cgccgttctg gccgtcgaaa atctcggcat agccgtttaa gcaaccttct tccagttctt
    15661 tttcgagcct taaaaaacaa tgacggacat attcgcgtga atcggtggaa aaatcattca
    15721 ccctgagaca agcttccaga taaactccga aaaggaaccc ccaggaagtt cccatatggt
    15781 aggcgcggtc tcttttatcg atactgccag aatattccgg cttgaaatta tcgttgtata
    15841 gagacaatgt cctgattccg tatatgtctt ccaattcctg tttggctaac tcccagatgg
    15901 aaatggcttt tttctttggc atgatatcga acggcaggct aatggcgtat aactggttcg
    15961 gtctgatcga cggatcgtct ggatcgacga catcgaaaag gcatccggtt ttctgattcc
    16021 aaaaacgttt gacgaacgaa tttttggcag tttccgccat caggagatat ttgtccccgt
    16081 cgattccaag caaagaagag aatttgacgg cgatcatgag ggaattatac caaagagcat
    16141 tgatttcgac cggtttgcct tgtctaggtg ttatcggttt atcatcaatc ttcacatcca
    16201 tccaggtgat ctggtcacta ccggatccag cggaaatcaa accgtcggaa tccatcctga
    16261 tatggaaatc ggtacctttc tcgtaccatg caagaatctc ggcgatgtca ggataaaatc
    16321 ttcgttttat gatggcaaga tccccggtag cggcgaaata gcgatagatg gcgatgatat
    16381 accacaaaga ggcgtcaacc gtgttataaa gcggtttagc gccggaattc ggaaaaacat
    16441 tgggaatcag gccgtttttg agatagcggg agaaagaaag caatacttcc ttggccatct
    16501 tgaatttccc ggtcgataat aacaagccgg gcaaggcaat catcgtatcc cttccccaat
    16561 cagaaaacca cggcagcccc gccaggatcg tggtggcgtt cgtttcggct ttcttggcga
    16621 tgaattgttc ggcagatacc catagtcgct tttcgaatgt tgtgcatgat ttggcagcat
    16681 tcagaatacc gtcaattttc gccgaatatt tcctgatgat ttcgtttgcg gtaaattcga
    16741 tggtttcgtc catcgacccg accaatccga tttcggtttc cttatccgcc ggaatcgtga
    16801 cgacaagttt gcagggagtg aaaaaacaat cgctccgatc gtcgccatta tatctgtcaa
    16861 gatcgtattc tccgatcctc gcatcctgct tgtcagtcgg taggatttcc ccttgggaaa
    16921 aagaaaggat tgtcacatgg cccggataca ggcgggaata gactttggct ttattgtctt
    16981 ccttttcata tgtcatatgg gcaaacggca tgttttctac ctgactgtgc tcacggaagg
    17041 caaaccaggg agttaatacc aacttgacaa tttctgggga atacacccga taattgattg
    17101 caacagcgtt tttcccgtat agcggcgcta tttctttggt gaggatcaga tttccgagcc
    17161 ggtatttcca tttgacggtt ctctcataac agaattccac aagccccgaa ctaggtccgg
    17221 atgtcttggc gcttatctct tccagggaca ccgtgccgga atcggtgtgg atttcttcga
    17281 tgattttgct gatggccatg acccgcctga ctggcggttc gagcgatgcc accaacaacc
    17341 cgtgatgctt gcggaaaagt tgtccggaaa ccggaccgga agcataacct ccgagtccgt
    17401 tggaaagcga ccactccaga cgactcattt cttctttttt tctgattgtt tcactatcca
    17461 gggaatacca cataagcaaa cgcctcccaa tcaattttat cacatcgggg catatcataa
    17521 atttatttta tgaattattt tgcttgaaaa ctacagaaat ctttttgtct ggcaaggtag
    17581 atatgttggc aatagcgctg ttttttggta taatatattg gtggtgatgg aatatgatca
    17641 tgatgaaaga tatcatccag gaaggcgatc cgattctgag aaaaagatcg gagaacgtac
    17701 ctctccccct gaacaataag gatctcgaaa ctcttcgcca aatgatggaa tatattgtca
    17761 atagccaaga cgaggaaata agtaagcaat acggcttgcg accggctgtc gggctttcag
    17821 ccccccagat cggattaaat cgcaaacttt tctgcatgcg ttgcccggat gaaaaaggaa
    17881 tagccatgca ttcttacgcc gtatgcaatc cgacaattgt ttccttttcg gaggaacaaa
    17941 cttatctcgg cggtggcgaa ggctgtcttt cggttccgga aaatgaaaac ggtctggtgc
    18001 cgagatccat gcggatcaag gctagaatgg tcttggtcga tctcgaaacc gggaatatgg
    18061 aaaaggtcct gatgaaactt tccggttatc cggcaatcgt cttccagcat gaatacgatc
    18121 acctgttagg cattcttttc gtggataagg taaaaccaga gttgccgaaa attaaaccga
    18181 tagcatttgt ttcggatgaa gccgaataat aacaaaaggc ctttgcgggc cttttatttt
    18241 gctttctttt cttttaggaa catttccgtt tccatgtaac caggcattga agcgagcgct
    18301 ccttttttgg ttgccacaag gctgccgaca gcattagaaa attccaatat tttttggtaa
    18361 tcggattttc ccagggattc tttcgttaaa ctgccggaca atatcatata cagaaacgct
    18421 ccgatgaagg catctccgca tccggttgtg tcaattgcat tgacgacgaa tccgtcatga
    18481 acgaaagttc tgtctttcgt gtacaccgct gacccgtttg cgcccatggt atatacgatg
    18541 actttcacat cgcctatgaa aagcgaggat aaagcgcctg ttatgtcttt ttttccggta
    18601 atgaattcta attcgtcttc gctgattttc agaatatcgg acaaaggaat aaatctccga
    18661 acaatctctc gcagttcttc cttgttttcc cagagcgaaa gccggatatt ggggtcgaaa
    18721 ctcacgatca atcgtttttc ttttgcaata cctatagctt tctcatgtgc ttttctgacc
    18781 gggaaatcca ccaggtcgac actacaaaaa tgcaggatgt cgccttcttc gaaaatatcc
    18841 aaatcgattt catcttgaga aagcaacatg tccgcgctgg gattgcggta aaaacaaaaa
    18901 tcccggttac catccggttt tagagatacg aatgcgagtc cggttgatgc ctcgtctgta
    18961 tgctttatgt aagatgtgtc gatccccgtc tttttcatct catcaatcag aaaatctccg
    19021 aaaggatcgt taccgagttt tgataataca acacttctgc cgccaagtct agcaactgca
    19081 gcggcgacat tggccggggc tccgccggct tgtcttctga aaatcgggac atctttcagt
    19141 ttccagtttc cggcatccgg aatgaaatca accaatactt ctccgatcga tatgcatttt
    19201 cccataaacc agtcctccgg gatggtaatt attctttcag tttctttgcg aatccgattc
    19261 cgaaatcata tgctgtttgt atttgtgaat ccgacggttt gaatctgatc ctcaagccgt
    19321 ctactgtttt cattttcagt tgtttcaatc gttccgtaac attttgtacg gcttctccgc
    19381 tccatccgta agaaccaaag gctccggccg tcttgccgcc gtctgtctcc ggattcaggc
    19441 gggacagcat gtcccagatc ggatataccg cgtcgcgtag gatggtcgta gtaccgatca
    19501 gaaagccatc gctttccccg attcttccga ctatggtttc gatatcggtc gtaaccacat
    19561 cgtatgtttc gatatcgatc ttaccgccaa actcgaaatt tatcgctttg gcgataactt
    19621 ccgccatcct ggcagtgtac ccatatgcac tagcaaatgc aataactaca aacggttttt
    19681 ccctcggttc gggctttttc gcccattcgg catatttatg caaagcttcc ttaatgaatg
    19741 tttcgtcaag aaccgcgccg tggcttgtgg caatgtattc gagatttagt tcttccaagc
    19801 cttcgatccc tttgaacacg aaaggcctga aaggtgacat gatcgcgtca aaatactcct
    19861 tgaaagcttt cgcaaaatct ttccggtcag gaacattttt ggcaagcacg ccttcgaagg
    19921 cataatgggc gccgaagaaa tcgcaagtaa aaagaatccc gtcttcggga acataggtga
    19981 aaatagtgtc gggccaatgc aaattgggcc tgatcatgaa cttcaaggtt ttgtttccga
    20041 gatcgattgt caagtcgggt gtcgccctaa gcgatttgaa aggccgattt agtatctctt
    20101 tgagatttga caggcttgga gcggatccaa caatcgtgat atcgggattt ttgtcaagaa
    20161 tatcggcgat tgatcctgaa tgatcgggct ccgtatgatt gacaatcagg taatctatgg
    20221 cactgaaatc aaccagtgag ccgacctttg ccagataatc ggcgctgaaa ttagctttga
    20281 cagtgtcgat aagagcgact ttgtcgtttc ctttcacgag atacgcgtta tatgaagaac
    20341 cgaattcggt tctcatgatt atgtcgaata cccgcaattc ataatcgttc acaccgaccc
    20401 agtaaattcc ttttttcagt ttgtaagtat tcttttgcat gttctcacct cgcttatatt
    20461 ataccgcaac aagctatata gccaaagaat agagtcttca ttttgtttgt ttaagaatca
    20521 aaatggtgca atttcataaa aaaagttgtc ttaccgacaa cttcttttta ccttatcccg
    20581 taaaaatagc ggaggatctc atccatcgcc ggatattcca acaaacggat gtcatctgtc
    20641 actttttgca catcatatgt gatcgacaat tgttcccagg ttgtttcttt gccaatgtca
    20701 agcacgatgc cgcgggtctt atccaagttt ctgccggggc acccgagcga tcccggattc
    20761 agatacaagg ctttcccgtt tgcgacaaag cctttgtgat cgtgtccgaa aatcacgata
    20821 tccgcatcga tattactaaa aatcctgtcc atgtcggaag atgatgcttc atccgatata
    20881 tccatataac gactttctcc gttcatggcg taatgggcta tataaagccg tttccccgct
    20941 atattcaaat atgtttcgca gggcaacgat ttcaaataat tcccggattc ttccgataga
    21001 aatgcatgtt cccaaacgtg gtgctctttt tcgacgtcac tcatttccct gacaggaatg
    21061 ataccttccg caaaatatct ttcatggttt ccggcaacac aggcaaacag gttcggtatt
    21121 ttcatgaacc gcgagacagt ttcttccgga tatggtccga tgccgatgat atcgccggca
    21181 cagatcaagc cgtcgcattg tctttttgca aattcctcaa gcattgtttc caaagcaatt
    21241 acattattgt gaatatctga gattattccc aaccgcataa tattactctt tcaatcggac
    21301 atcatgtcgt atatgtgccg atgaagcgat tgtcatttcc gacaataaaa gcgcggattc
    21361 cgttcatcca ccagccaaag atatattcaa gataaatcgt gaccgtttcg tcctccaaag
    21421 aagaaagatc aatttccaga ttcttcgtga atatagaacc agatatcatc aattttggtt
    21481 tttgattgca atccagttcg atttcttgat tgtttcttag atcgtttctg aattcattgt
    21541 ctacatatat atccatagga acgaggtaaa atcttggact tttcagtctg ataaaatgca
    21601 atttcatatc atacacctcc gctttctttt gcaaatatga tatttcccgg aacaataggc
    21661 cacaaacata aggcggttaa aagtgcaaag ggaaacagat acatcggcgc tattagcagg
    21721 accagctcat aaaaaatatt gctattatgg taaggctgtt ttttcaaacg ggagaaaaat
    21781 gcgctcatgg gcaagccgaa aacgttgccc atcatcatga tctgaatcgt agccaaaatg
    21841 gctttggttt tctttttgcc gtattcttcc agcaaaacat cataagcata atgttccgga
    21901 cgtccgttgg aatcggcata gtgctgggcg aaaatgatcg cctttgcttc cgtttccttg
    21961 atcatttctc cgtctccctg gagaaatgaa gcaatctcgg cagaatccat gccttgcttc
    22021 agggcaacct gcgtatgggc ataactgcag acctcgcatc cgttgacttc cgtaacggca
    22081 agcatcagtc tttcctggaa atgcggatcg atccggtgtt tcagtctgct tgcggccatt
    22141 atgggaaagg cccgcatgct ttggctaaaa acgatcaaca cttcccacag ggaatagcgg
    22201 tgcctgtacc ctttttgcat gatataagcc tccttcgatc aggcaataaa tattgcttgt
    22261 cggtacaatt atacaatttt tggctctgaa atatagcgat aacgctcata tttattccag
    22321 aacgaatttg tgcgaatatt tagaaagatc gattttaccg tctttgaatt ccacgccttc
    22381 agcctcaagt ttttgtcttt gcatcgctcc gccttcgcct gaaagtccga tttccccttt
    22441 ggagttgatt accctgtgcc atggaaggtt ataagaaatg ctcgacgagt tgagaattct
    22501 caccacttgt ctagcgccgt aacgattccc ggcataactg gcgatgctac cgtaagtcat
    22561 cactttacct ttcggaatct gcttgattac cgttatgacg ttttccgtga attctgtcac
    22621 tttttctcct ttttacaacg cggactatcc ggatgatttt ctgttctttg ttaaaataat
    22681 tatatcatga aacgacaaac gaaaaaactg tgttgtcaaa atgatacttc atgcaatttg
    22741 atttttttta acctggatat ggatataagc atgttatatg gcctttcgca tgttaatgtg
    22801 ttataatatt cttcgaataa atcgcctttt atggcttctg tttgcattgc tgccaagcat
    22861 cattgaagat atgttcgacg ggaggagggc gagcgatgga caaaagagga ttagttttgt
    22921 cggttctggg attatcagta tcttttctaa cccttgtcat tgccacattt gcctggttcg
    22981 ccatcagcac aaccgtggac aattccatgc taggcatgaa tgtaggagcc ggtatttttc
    23041 catcttacga tatccattat tacacatacg atcatgtcta taaatacgac cagtcttccg
    23101 aaagcatctt ggtatacgac ggcggattct gggtagcacc atcctatgaa gtgctaccgg
    23161 aatcagccta tgcttttcag ggaatcatga tcggatccta tgatccgctg gttcccatca
    23221 ataacgaata cgacaacatc atcatcgaat tgcatcttta ttgcgatgcc gatgaaagca
    23281 tgagtttttc tcttggggct tcggcggata cttctatggc cgctggagcc gttttgactt
    23341 tcggagacgc cggcacaatc tattatttaa gtcaaattgc ttatttgcaa gaaatggaaa
    23401 ccgacaatta ccaagctaac ccggaaggga ccaatatcta ttcatcatta aaatcggatt
    23461 ttgaaaccgt ggacggaaac gaagatctta tctatccgtt gcatggcttt tatgacgaat
    23521 acgatgctta tgtgtctgaa acatccctgg gatcgctttc tgtaacgagc gctactacgg
    23581 atgtttatct ctattttaat atttcctata acacagctaa aatcgacacc attctatcag
    23641 gagagggttc gggatcgacc ataacctctg ttgtcgccct gcgcttctac caggacatca
    23701 aaatatacat tgaggaggct tccgggtcat gacaaggaaa aaacggaacc ttcttcctat
    23761 cgtctcgctt ttggtcgctt tgctatttgt tgtttccctg gccgtcacca catacggatg
    23821 gatagtcaat tactattctt acagtcaaga tgttctggga aaatcaggag cgctttattt
    23881 cgccgatggc gacggatcgt catccgaccc tttcatcatt tccgaaccta atcattttta
    23941 taatcttgcc tatctccaga atatcggcgc ttttggtagc accacgtatt atttcaaagt
    24001 cgccgatccc gatacaggat tggctatcac gattgatttc agcgatccct cgtatctttc
    24061 aacttatcag acagtttatc ctatcggagc ggaccctgac gcct
//
LOCUS       DAOPJL010000020        23686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_2267_length_23686_cov_8.598959, whole genome shotgun sequence.
ACCESSION   DAOPJL010000020 DAOPJL010000000
VERSION     DAOPJL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23686
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2267_length_23686_cov_8.598959"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLP02_03795"
     CDS             complement(<1..677)
                     /locus_tag="PLP02_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF42606.1"
                     /translation="MNKAKLGTRTWSTLLLFGLFGQLAWAVENMYFNLFLYNEISGNP
                     HAIAWMVAMSAATATLTTMIMGALSDKVSKRKIFISVGYILWGISTLAFAFVSVDTVA
                     SLFKYGDAILITVVIVIIMDCIMTFFGSTANDGAFNAWVTDVTDSENRGKVEGVLATL
                     PLVAMLIIFGGFDVFVQNDQWPLFFLILGSLVSLGGIIGLFLIKESDVRKSEENYWKT
                     LIYGFRPS"
     gene            complement(771..1694)
                     /locus_tag="PLP02_03800"
     CDS             complement(771..1694)
                     /locus_tag="PLP02_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPF42607.1"
                     /translation="MNKLQKIIDKFQITEVETLNKGWSTDRKFILADKNKTKYLVRIS
                     DILLYDKKKMQFEYLKKFEAKGIPCPRAVDFGILNDNEVYLLQTWLDGMAAEDVVPTL
                     SDMEAYNLGIEAGKTMKKIHSLEIPEQSRTWGERYRAKMPKKIDKLLNCEYELPMQEK
                     IIRYYQEHAHLAYERPLAFSHGDYHVGNMIVDNNAIGIIDFDKSEAADPYDEFKPFCW
                     NVFASEYFETGMINGYFDNEIPDDFFPILKLYAAESMVSFLPWSVTFGEAEVKEAIKI
                     TEHVLEWYDFFNRDIPSWYKGIKAEFISEKK"
     gene            complement(1759..2205)
                     /locus_tag="PLP02_03805"
     CDS             complement(1759..2205)
                     /locus_tag="PLP02_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42608.1"
                     /translation="MDNMTWESDRFDIEATSEQLRMQYETTGDSIFELDSEYYSTFVI
                     DGNSYYCIIYLVNGYFSLYLAQPELLEDYYDYYEEGVPFLFGVFEDEKTKNEDYQYRL
                     IVTLDKDDSAFGTTDGEEAYTAYYQYCTANDIQYDDELILYGYKSS"
     gene            complement(2561..3160)
                     /locus_tag="PLP02_03810"
     CDS             complement(2561..3160)
                     /locus_tag="PLP02_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF42609.1"
                     /translation="MNIILGGALTNNQVYDIYNEYIIETYQNIDMQSNEHYKDLTELI
                     YNKHFKDKSYDLVIGHSMGGLIILDILCRNLEIKCKKIVIVESYIFEPSKEFRNIVYK
                     NIHLEQKLNNVMRNEVEKYNSKIFDSLRKLNIVEDLKDISRTLLFVYGMRDLDNDTFL
                     QKLDFPNLQNVRLIGIPQTSHFCLIEKPTEFLKKILNDD"
     gene            complement(3302..4618)
                     /locus_tag="PLP02_03815"
     CDS             complement(3302..4618)
                     /locus_tag="PLP02_03815"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF42610.1"
                     /translation="MLKRKIEELLILWKNEKGKKAFLVKGARQVGKTTSIRAFGKSQY
                     KTFIELNFERNPDLCNIFSGSLDARTIQLNMSAMGYGPFVPGETLIFFDEIQACPKAR
                     TAIKFLVEDGKYDFIESGSLLGINYNDVSSYPVGFETQHEMHSLDFEEFLWAKGVGEE
                     VIHELRKCVSEKKELSNAIHQTMMRHFREFMIVGGMPSVVSKFVQSNDLTIVIQEQKD
                     IINSYRDDVKKYAGSNKTKVSEIFDSIPAQLNKKNKRFYLSALKKEPKMRTYEEAILW
                     LFDAKIASPCYNIAAIELPLALNEKRNLFKVYMNDTGLLTALSYLGIQSEILNGNIEI
                     NEGSIIENALADAFLKNGFLLHYYDRKKPTSMEIDFVIQVRDTVQVIESKSGSDFEKH
                     DSLNTLLKEKNIGEPIVFCTENIHETNGILYLPYYLGFALRNDDLR"
     gene            complement(4785..4858)
                     /locus_tag="PLP02_03820"
     tRNA            complement(4785..4858)
                     /locus_tag="PLP02_03820"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4824..4826),aa:Cys,seq:gca)
     gene            complement(4906..4982)
                     /locus_tag="PLP02_03825"
     tRNA            complement(4906..4982)
                     /locus_tag="PLP02_03825"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4946..4948),aa:Pro,seq:tgg)
     gene            complement(4992..5068)
                     /locus_tag="PLP02_03830"
     tRNA            complement(4992..5068)
                     /locus_tag="PLP02_03830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5032..5034),aa:Arg,seq:acg)
     gene            5198..6004
                     /locus_tag="PLP02_03835"
     CDS             5198..6004
                     /locus_tag="PLP02_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF42611.1"
                     /translation="MDAKEVVVMIKEDIRIGMVGIPVADPIKESEFYRNLFGLSETLE
                     NAANHPLRFLKTDFTEEKTFEHLALKVRSRAELGRILRRLINLQIPILGAIDHGYAES
                     IYLNDPEKNVIEIASLRDAQEWYDEFGQIKDSSSPFDYSGVYYAAEAEDAIDDSGISV
                     AICHIVLNVPDLDKARHFYEQAMGFAVRSEKKGKALLLTASQCHHQIGLAATKKISKA
                     SAVFSISYPVCESLMQTIERIRGKGFLVNESEKGYLVTDPFGNMIYLVLG"
     gene            complement(6051..7070)
                     /locus_tag="PLP02_03840"
     CDS             complement(6051..7070)
                     /locus_tag="PLP02_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPF42612.1"
                     /translation="MDTLLFALNAVLPLLLMIGLGYILSRTRFLGEEFWPCANRFVFR
                     IALPCLLFYTVYSASGLQDIRWNVVLYASAGVLVLFVIGFLVAMFFVKDPTQKGVINQ
                     GVFRSNMTIIGVPLAEALGGADAVLVVALVSLVIIPLYNALSVIALSVFLRGEDGEKV
                     PWIKVLLKILSNPLIIAIVLGILSLYIRSLIPVFASTGEPVFSLKNDLTFLYTCIKWL
                     GQIASPLALVVLGGGFQFAQVRAMKREITLGVLLRTIMAPGLMLTLAVILAKYTTFFA
                     FESTDYPALIATFGSPVAVVSAIMASEMKNDGKLAGQIVVWSSLFSIPAIFLIVVIYR
                     TLGLL"
     gene            complement(7169..7513)
                     /locus_tag="PLP02_03845"
     CDS             complement(7169..7513)
                     /locus_tag="PLP02_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HPF42613.1"
                     /translation="MKNRIAAGLFAIFLGGIGVHRFYLGQVGLGLIYVLFCWTGIPAI
                     IGLVEGILILTMTDEQFRDKYELGVVVSTDNVLATKTVSAADEIKKYKDLYDSGAITQ
                     DEFEKKKKELLK"
     gene            complement(7515..9215)
                     /locus_tag="PLP02_03850"
     CDS             complement(7515..9215)
                     /locus_tag="PLP02_03850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPF42614.1"
                     /translation="MKFFRSVLRKISGFFGTYYDENEQDHYDVAMGIVSASIIAIFEI
                     ISFIAVDPSLLRSKYYFLLTTGLMIMQLVLFSLIHAGAISFDSWVRKATVHAHPYLIV
                     SVGIIISFAYQGYSNQIYSFIIAVFATSLVQLYPLWKRLSVFLYALIAFNLMIWITEG
                     FQHFFTENLRLSVMSVVLAYVFTLVQSRSDLGQRKMTAILRRQNTSQEEALGRLRKAY
                     DDIELNYRITEMMLTITSEMLNTDFFDDVLQMILEEAVKAVPKVSSGSILILNGEMME
                     YRASVGYDLKTLQRTKLKYKDVFQSDLEDPFEPTIIRDLQKYDEKKLSADTIKLLRSG
                     NALGAMSVLTCSFKHNGKFMGSINLDSDLSDSIFDESDKQMIKQLAKQIEIIITIHEL
                     YGDAIRRVRYDYLTDACSRSYHQELLKLAYNEAIENKTPLAICSIDINNLKEVNDSFG
                     HEAGDDLLIHFADGVRKNAGNNFFLSRVGGDEFFLVFPNATEEKATDRIKQIRDYFND
                     NPFMVQGNAIQVVFGCGVVCCRDEKCDLDMLVKLSDAKMYQNKAELKKQKDTIDQLEE
                     "
     gene            complement(9384..13271)
                     /locus_tag="PLP02_03855"
     CDS             complement(9384..13271)
                     /locus_tag="PLP02_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HPF42615.1"
                     /translation="MKKRIVLAIAIIAMAFVLVSCKDETTAVTTLTTTEAFTVTTTTT
                     ESDLSIRLHQIYDLGVASGDLPGTYEEWLESIQGEQGIPGEDGRDINLQVASGYVQWQ
                     YVGDTTWTNLIALSSLIGADGADGIDGTDGEEVMFQVFDGYIQWKYESDVSWTDLISL
                     AIITGADGTNGREVTFRVSDGYIQWQYVGDASWLNLIEVSSLVGSAGETGVGISSMAI
                     NDQGELIVTYTDGTEKNLGQILVTYLVEFKNFDGSVLDIQHLTYGSAAVAPADPVRAG
                     YVFTGWDHSFSSITGNTVVTATYSPETFTVAFDTMGGAMENAIAGVPYGTTIILPYPV
                     KEGYSFLGWYTGTGVSNSQFFENTLVTEDLILYAKWQTATSVTVYDYESLTAALDNFS
                     YDEIYLGADIYAMSSVYIDRQVSIYGNGYTLYSYAGSNGFISIGSHYDSERDYYNKYA
                     IIEGSSIQINDLYLEVAPGLDNDTSYDTAIYIGYTYPFNLVLNRVNSSKGMFSFLYME
                     CPEGLNLEMNNCDIAALYGMYFYGDENSNIVINNTTITAMNAIALEYTYYGYFTINNS
                     YLYGGMIYNDNFDLDEGTFSMTECRENQIQIDNSVLSGKAIGDYYNPLILSESESSSS
                     NLYMTECTYNLPVANIDGMIYSSDGLLSTSFERTTVVIPEGTTSIEANAFYGAEEINR
                     VILPDSVISIGEYAFAECYGLEQINIPDGLISIGEDAFYNAYDLNYVIIPASVETIGF
                     EAFYDNSYLTVYAEASTIPAGWDAGWINGGYPVEWDIAGYTTENGLTYVVLSNNEAIL
                     LGSDTYTVTSITIPEAITISGTAYPVTSIYSYAFYEQYDLSYVYFASNSNIVTIKEGA
                     FFACGNLKMISIPVSVVIIEPYAFEQCYKLAQLIFEDGSVLQEIGDEAFSATNLHSVT
                     IPASVTYIGYMAFGDCYSLTHVYFAPSSNLNQIGESAFSNNNLYEITIPDSVTIIEGH
                     AFYGNYRLASVNFGENSQLQSIGSNAFQSCALTTITIPSNVLTVGDFAFGWDTYLTEV
                     IFAPGSQLQDIGQYAFSFTALTSITIPASVTHIYNYAFLNCSDLVNIVFEEGSSLIYI
                     GTYTFNACSALESVEIPATVATLGDYAFQDCTSLTSVSFQFAGEGDLTSIGNYVFKGC
                     TSLADFYIPDTITSIGNNAFYNCGSMTSILIKNNVTAMGRDVFVGCTGLTINVEASSK
                     PAGWDASWNSSGLPVVWGCFNTTIAFESNGGTAVDSIWQEQGTAVTEPVAPTMDGYVF
                     DGWYEDVALTIPYTFSTMPYEDITLYAAWTLAE"
     assembly_gap    13414..13513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13518..13593)
                     /locus_tag="PLP02_03860"
     tRNA            complement(13518..13593)
                     /locus_tag="PLP02_03860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13558..13560),aa:Glu,seq:ctc)
     gene            complement(13600..15258)
                     /gene="gltX"
                     /locus_tag="PLP02_03865"
     CDS             complement(13600..15258)
                     /gene="gltX"
                     /locus_tag="PLP02_03865"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPF42616.1"
                     /translation="MDSNHKLADLIFPNVSMTLDDLETKYPERKLPSGAMVTRFAPSP
                     TGFLHTGSLFASMVSWRFAKQSEGVFFCRLEDTDQKREISGSADKVLAELLTFGIVPD
                     ESFLSGGAYGPYVQSERKWIYDIVVKYLISRGLAYPCFCSHEELDAIRKEQEAKKLNS
                     GYYGEYAKCRFLTAEEAAERIKKGNSYVIRFRSPGNEENRIAFDDAIRGHIEFPENIQ
                     DIVIMKGDGLPTYHFAHLVDDHFMRTTHVTRGEEWMSSVPIHLQLFDTIGWKRPVYAH
                     LPVIMKMDGETRRKLSKRKDEEASVSYFLEKGYPVEGFLEYLMTIANTNFEEWRIENP
                     EADIFSFHLDFSKMTLDGALFDLEKINHIAKERLGAMSASEIADKADEWAKIYDHELH
                     QRITTDPTYFEAILAIERGGEKPRKDYTRYSDIFPVIGFMYTDIFLASIKEGLTIIDR
                     FPTELVKTSLKEFLDNPGLDLSEEDWFGNLKATASKCNFAEKPKDYKKNPEQYVGHVG
                     DFAEIIRVAVSGRKNTPNFYYILRILGLDKIRERIYHTISLLPD"
     gene            complement(15322..16995)
                     /locus_tag="PLP02_03870"
     CDS             complement(15322..16995)
                     /locus_tag="PLP02_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPF42617.1"
                     /translation="MRNRILFGVSLFTLVLYMSMQSIWSGIEGMFGVSWLQYVILGLI
                     LGATVVLFMPMVIKTKKNWVWIPGGLSIALGIAILYMLYLGIGSFHYIFRSFIEYVMI
                     LAGTTFVLYLFFYFPKTKLWADSVFRIVLISVMILILGAVSFFSGFVLISTHPTVFAV
                     EDEYQIVWTTSIDATGLVKIGNDTYVDTYSGSADSFTTVHKVVVPMSVLDQAGEYEII
                     STNYLYRGPYSGLEGHTVTETYEFRPVDLSDGIQIYTIADAHEYVGAASKTGTYFGDS
                     LDLLVMAGDIASFLEKESDIKIIHRIAYNITGGTRPVVYARGNHETKGEWADELYKYV
                     GSVNESFYFTFNLSGIFGIVLDLGEDHPDDWWEYYDTAYFTEYRDMQTEFLNDVIAKD
                     AYSDPGVVFKLVICHMPITNVYSDTSDFGSGIYFLETIKNLWTDLLNTIDIDLSLSGH
                     THQLMQFLPGMPVNETLYYDEAYYDTTKSHGYMTDADFPAFTVSRRSDVQTPYVKENL
                     FGQKLIGMATTIDLANSEMHMLYTDSNLQVVDIVVPFTGESVTGFDLPL"
     gene            complement(17160..18119)
                     /locus_tag="PLP02_03875"
     CDS             complement(17160..18119)
                     /locus_tag="PLP02_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPF42618.1"
                     /translation="MRKFLEDLRQELKNKKVVDSIIDEIIVDHEEMIANAIEEGLAEE
                     DIEKKFGDPLKLASQLAEDLENEKEEINEEGALKMTLWKEFALQSDTLDVETRFVNDD
                     IEYTSADIETIQVFWTKCRRPEDYEAEYDGKTLRLTYKRKNMIFNFNTTRKGIKFRVL
                     LPKSVIVANFKHVGTNSDVEYRNIKIGKLSINTTNGDFVIDNVEAGETEWHSVNGDFK
                     VNNSRLSGLSATMVSGDIQIVDTNIENDMEIGTVSGDVSVKNTKVKELDYHTVSGDFD
                     GKEFYPEKVTLRSVSGDINIHNSEYNKIEIVSKKTVSGDIYIR"
     gene            complement(18076..18438)
                     /locus_tag="PLP02_03880"
     CDS             complement(18076..18438)
                     /locus_tag="PLP02_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF42619.1"
                     /translation="MNAQFKRGIVELCVLSLLAEKDMYGYRIISELAKNIDVNENTIY
                     PILRRLTAEKYFDTYLEESPDGAPRKYYQMTDKGFRYYLKLRNEWDAFIGGVYHIINQ
                     GGKKNEEISRRFASRIEK"
     gene            18599..19726
                     /locus_tag="PLP02_03885"
     CDS             18599..19726
                     /locus_tag="PLP02_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42620.1"
                     /translation="MIDKRIALAEKMALRDPSNIKTVIGFDGFIDEIIHVVDKREDSE
                     HFTRIGKINDLAERIARAGGLSTNIELFPTTRKLGGNGPIMANALSMHKCHQTYIGAL
                     GVPVIDSVFAEMATKAEVYSLTSSGHTDALEFDDGKLLLGKMSSLGDITYDRLVLAVG
                     FDRLVKLLEEADLFATVNWSMIPHMTELWKHLLENVVPRLSDKKKKPVFFVDLADPEK
                     RKDEEIAEALEVIAGFRKHYFVILGLNKKEAYGLAGSLGLFSSKSLSEDLSLKEVNQA
                     LYDFIGIDCVVIHPVDRSSTVAGGNFYEEFGPYIKTPKLSTGAGDNFNAGFVLGIMLG
                     FSPDEALLMGMGTSGFYVRKAKSPDYAELTGFLRDWAENKI"
     gene            complement(19622..22177)
                     /locus_tag="PLP02_03890"
     CDS             complement(19622..22177)
                     /locus_tag="PLP02_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPF42621.1"
                     /translation="MKIYDIGKIPADLLNFVGGKARGLYYLSQIGLAVPEGFIVTDIK
                     SDSDVMEAAEYYEKSNLGAVAVRSSATAEDGGSSSAAGQYDTVLNVAGKDNFIQAFHR
                     CLESLDSERVRKYNEIFLNRQVSEMTVIVQKYIDAKISGVAFTIDPLNDHMTLIEAVE
                     GAGENLVSGSVSAIQCRHDGTEWIIPVNCFIDESHLEEIRIGSQKAKGYFGCELDVEW
                     VVSKTGRLYWLQARPITTLDTPTIHELDTVKDYTKRVITTNNISEMMPGAVTPLTLTT
                     SMYGIEWGMRKLLVVSGTFRRMDDIPPGSCIVSNGNHMFIDLTTLYMMENMMILTDKQ
                     GVDISICGRSLEASENINLEKAGFIRKLVNFVRYASLLFKKEKYARLHEKLYDDLTFD
                     PQAPTKIFYENIDANLTQLNLSLFYHYVSSCYSGSMLSALNAALEPDFESPEKARAAV
                     ASVLENIDGIESVDILMSLRKIAKQVKKQFPDVDGMEKKDILSFLENPANDINRDYIS
                     FMERHGHRSVREAELRSRSWRDEPLNLAGMVKNIAAGGVFDTETDMNGWRKNRDMLLA
                     NYGFAKKKIFRYLINSSRDGVRYREKSKSMIIKVIDKFKHAYRNLAGKLVEEGLLPDE
                     DCIFFLTHQEIGELIDKKSSIFAKQALVRRRLLPEQMLLHFDDVTIGKPEPILLNRKI
                     HSTKLFKGTPVSNGIAIGKARVVRSLEDANELQKGEIMVAGFTDIGWSPYYCLLAGLI
                     TEVGSALSHGAVVAREYSLPLVSNIEGATGIIATGDYLHGCQEWGSKNNNRRRSQGID
                     RRRKIKAAGIFPAVLILSNSDFVFRPVSEKTGKFRVIRAFCLADVKPARTHAH"
     gene            complement(22187..23212)
                     /locus_tag="PLP02_03895"
     CDS             complement(22187..23212)
                     /locus_tag="PLP02_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42622.1"
                     /translation="MKCMDAIKESLICHSLKKRSKNRKYTREEAERYVLPDDAGSEYN
                     NSYYFSAHDLSGMSLFCRLGQRGGKSADEVWFAFADKKNRYNLKQQLFENDIPLKVRC
                     LEPGSAWQVEFTGFLEDAKTKKNVEAAFSGIFRSDEPIFDFSYDMDPRPMAKTIAKAK
                     WTKAFFKELSENNQTHYEQGGRLEGTFTISGVKKKISAPCIRDHSFGKRSWDYMNMHM
                     WLAALDENGKTINFSMVSYPALDEIWVGNTNIAGGNHKSVIGHEMRTDIRNNNLGQNT
                     VKAIVETADKEKYVITAERTDVFDYVFDGGKYHLYEGLGNFTINGKPARGIIEFGFND
                     DESRWRK"
     gene            complement(23473..23548)
                     /locus_tag="PLP02_03900"
     tRNA            complement(23473..23548)
                     /locus_tag="PLP02_03900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23513..23515),aa:Glu,seq:ttc)
     gene            complement(23552..23628)
                     /locus_tag="PLP02_03905"
     tRNA            complement(23552..23628)
                     /locus_tag="PLP02_03905"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23592..23594),aa:Asn,seq:gtt)
BASE COUNT         6501 a         5397 c         4820 g         6868 t
ORIGIN      
        1 gatggacgaa atccgtaaat aagggtcttc cagtaatttt cttccgattt cctgacatcg
       61 gattccttga tcagaaacaa tccgatgata ccaccgagac taacaagact tcccaaaatc
      121 aggaagaaca gaggccattg gtcattttga acgaagacat cgaatccgcc aaatattatc
      181 agcatggcca ctagcggcaa agtagcgagc acaccttcca cttttccccg attttccgag
      241 tcagtgacat cggtcaccca agcattgaag gctccgtcgt tggccgtcga accgaaaaac
      301 gtcatgatgc aatccatgat gatgacgatg acgacggtta tcagaatagc atcgccgtat
      361 ttgaacaagg aagcaacggt atcaacagaa acaaaagcga aagccaaagt cgagatgccc
      421 cagagaatgt agcctacgga aatgaatatt ttgcgcttgg aaaccttgtc ggaaagagca
      481 cccataatca ttgtcgtgag tgtagctgtt gcggcgctca tcgcgaccat ccaggcaata
      541 gcgtgcggat ttccggaaat ctcattgtac aaaaatagat tgaaatacat gttttcgacc
      601 gcccatgcga gttgtccgaa caagccaaaa agcagcaagg tcgaccacgt acgggttccg
      661 agttttgctt tgttcattga ttatcctcct gtcatttagc tttgcgcgag cttttatcgg
      721 caaaagccga taatattcat aatgaatata acacagatat aaagaaaagt ttacttcttt
      781 tcagaaataa actcggcttt tatcccttta taccaggaag gaatgtcccg attaaaaaag
      841 tcataccact ctaaaacgtg ttcggtaatt tttattgctt ctttcacttc cgcttctccg
      901 aatgtcaccg accaaggaag aaaggaaacc atggactcgg ccgcgtacag tttcagaatc
      961 gggaaaaaat cgtccggtat ttcgttatcg aaatatccat tgatcatccc ggtttcaaag
     1021 tattcgctcg caaacacatt ccagcagaac ggtttgaatt catcatatgg gtcagccgct
     1081 tcgcttttat cgaaatctat gattccgata gcattattgt caactatcat gtttcctaca
     1141 tggtagtctc catgggaaaa ggctaacggt ctttcatatg ccagatgcgc atgttcctga
     1201 taatatctga tgatcttctc ttgcatcggc aactcatatt cgcaattcaa aagtttatcg
     1261 atttttttag gcattttcgc ccggtatcgt tcgccccagg ttctgctttg ttctggtatc
     1321 tcgagcgaat ggatcttttt catggtcttt ccggcttcga ttccgagatt atatgcttcc
     1381 atgtccgaca gtgtcggaac gacgtcttcc gccgccatac catcaagcca ggtctggaga
     1441 aggtatacct cattgtcgtt taggattccg aaatcaaccg ctctgggaca gggtattccc
     1501 ttcgcttcaa actttttcag atattcgaat tgcattttct ttttgtcata aagcaatata
     1561 tcggaaatcc ttaccagata ctttgtcttg tttttatccg caagaatgaa ttttctgtcc
     1621 gtcgaccatc ccttgttcaa ggtttcgact tcggttattt gaaatttgtc tatgattttc
     1681 tgcaatttat tcatcttcta cccgccttaa tggatgtcgt aacaaatgtg atttatctaa
     1741 aatgtcaatt ttgattgttt aacttgattt atatccatat aaaatcaatt catcgtcata
     1801 ttggatgtca tttgccgtac agtattgata gtatgccgta taggcttctt cgccatctgt
     1861 agttccgaaa gcggaatcgt ctttatccaa agtgacgata agcctgtatt gataatcctc
     1921 attcttcgtt ttttcgtctt caaacacccc aaagagaaaa ggaactcctt cttcgtaata
     1981 atcataataa tcctcaagta actccggttg cgccaaatac aggctaaagt agccattgac
     2041 taaatagatt atgcaatagt aggaattgcc atcaatcaca aatgtcgaat agtattcgct
     2101 atcaagctcg aagatactgt cgccagtggt ctcatattgc atacgcaatt gttccgatgt
     2161 ggcttcaata tcaaatctgt cggactccca cgtcatatta tccatgtttt gataccttat
     2221 atccgtacat cccagcaaaa tcgatatcat caacagggca aacagcgcta atgttttctt
     2281 aatcgcactc tcctccgttc ctctgtattt aattgataca acgatgattt gtatatggaa
     2341 agcatatcat tttacaccgc atctgtgcaa ggggcttttt ggtttgtggc tatgacacac
     2401 tcagcttgtg tctttaagaa aacacccggt agataggtgt atgtagcttt ttgtcttgtc
     2461 cgtgctaact ttatcttgat tcaatacccc gatgtataga gcttattttt tgaatgctat
     2521 gagcataatt ttttatagtt caaatccggc aggatactat tcaatcatca tttaatattt
     2581 tttttagaaa ttccgtcggt ttttcaatta agcaaaaatg agaagtttgt ggaatgccaa
     2641 tcaatctaac gttttgaagg ttaggaaaat ccagtttttg taaaaacgta tcattatcta
     2701 aatcacgcat gccatatacg aataataatg ttctagaaat atcctttaaa tcctcaacaa
     2761 tgtttaactt tcgtagagaa tcaaatattt tggaattata cttctctacc tcatttctca
     2821 taacattgtt caatttttgc tcaagatgta tattcttata tacgatattc ctaaattctt
     2881 tacttggttc aaaaatgtaa ctttcaacaa ttacaatttt tttacatttt atctcgaggt
     2941 ttctacaaag aatatcaaga ataatcaatc cgcccattga atgtccaatg accaaatcgt
     3001 aagacttatc tttgaaatgc ttattatata ttaattcggt caaatcttta taatgctcgt
     3061 tactctgcat atcaatattt tgatatgttt caataatata ttcgttgtat atatcataaa
     3121 cttggttatt tgttaatgca ccgccaagta ttatattcat actgaatcac ctctataacg
     3181 attatagcaa aaaaagattt caattagaat gaaatctttt atagtttcga tgtcttgtca
     3241 taaccaagtt tgtatatttt ttggggttgt cacaaccaag ttttcttgaa acgaggtaaa
     3301 gtcatcgtag atcatcatta cgcaatgcga agcctagata atagggcaga tataaaatcc
     3361 cattcgtctc atggatgttt tcggtgcaaa agacgatcgg ttcgccaata ttcttttctt
     3421 tcaaaagcgt gtttaatgaa tcgtgtttct caaaatcgga acctgatttg gactcgatga
     3481 cttgaacagt atccctaact tgaatcacaa aatcgatttc cattgacgtc ggttttttcc
     3541 gatcataata gtggagaaga aatccgtttt tcaaaaaagc atccgccagt gcattttcga
     3601 tgatggatcc ttcgttgatt tctatgtttc cattcaagat ctcgctttga attcccaaat
     3661 aggataaagc tgtcaacagg cctgtatcgt tcatatagac tttaaaaagg ttgcgctttt
     3721 cgtttagtgc aagtggtagc tcaatagccg cgatattgta acaaggggag gcgatcttgg
     3781 catcgaaaag ccacagtatc gcttcttcat aggtgcgcat tttgggctct tttttcaagg
     3841 cagacaaata gaagcgtttg ttcttcttgt tgagctgagc tggaattgag tcgaaaatct
     3901 cactcacttt ggttttgttc gagcctgcgt atttttttac atcatcgcga tagctgttga
     3961 tgatatcttt ttgctcttga atcactatgg ttaagtcatt cgactgaaca aacttggaca
     4021 caactgaagg cattccgcca acgatcatga attcacggaa atgacgcatc atcgtttgat
     4081 ggattgcatt gcttagttct tttttttcac ttacgcattt tctcagttca tgaatgactt
     4141 cttcgccgac tcctttcgcc caaagaaact cttcaaaatc gagcgagtgc atttcgtgct
     4201 gggtttcaaa gccaacggga taactgctta catcattata attgattccc aacaaggaac
     4261 ctgattcaat gaaatcatat ttcccgtctt caacaagaaa tttgattgca gtcctagctt
     4321 ttggacacgc ttgaatttca tcaaaaaaaa tcaaagtttc tccaggaaca aaaggaccat
     4381 atcccatcgc tgacatgttt agttggattg ttctggcatc cagagaaccg gaaaaaatat
     4441 tgcacaagtc aggattgcgt tcgaaattca attcgatgaa ggtcttgtat tgtgattttc
     4501 caaaagccct aatactggtt gtttttccta cctgtctggc tccttttacc aaaaaagcct
     4561 tcttcccttt ttcgtttttc cacaaaataa gaagttcttc aatctttctt tttaacattg
     4621 taacacctct tcaatctcat tatacacctt tttcatgagt tttcaataat atatttacac
     4681 ctttttcttg aaaaaaccta tagtaaatta cacctttttc ggaaataaga aatgtacggc
     4741 ttgttagaaa aaaaacactt gtttaacaag tgttggtcag tgattggagg acccaaccgg
     4801 atttgaaccg gtgatcaagg atttgcagtc cattgcctta ccacttggct atgggtccct
     4861 ttttaaatat gttatgtagc taggatggca tacgtgattt tacgctggtc gggaagacag
     4921 gatttgaacc tgcgacctcc tggtcccaaa ccaggcgctc taccaagcta agctacttcc
     4981 cgataaaaaa atggcgcgcc catcaggagt cgaacccgaa accttttgat ccgtagtcaa
     5041 acgctctatc caattgagct atgagcgcgc agcggccttt tttaaggccg atatatatat
     5101 taccaaaaag cgactgaaaa agcaacttat ttttccttaa tgcattcgca tggtttcccg
     5161 agcaaatcgc tcggataata cgcggatatc ctgtataatg gatgcgaaag aagtggttgt
     5221 catgatcaag gaagacatac ggatcggaat ggtcggtata ccggttgcgg atccaatcaa
     5281 agaaagcgaa ttttatcgta atcttttcgg cttgtccgaa accctggaaa atgcagccaa
     5341 tcatcctctc aggtttctta aaacagactt tacggaagaa aaaacgttcg aacatctagc
     5401 gttaaaggtc agatcaagag ccgaacttgg acgtattctg agacgcctca tcaatttgca
     5461 gattccgatt ctcggagcca tcgaccacgg atatgccgaa tctatttatc tgaatgatcc
     5521 agagaaaaac gtcatcgaaa tcgcttcttt gcgagacgcg caggaatggt atgacgaatt
     5581 cggacagatc aaggattcta gcagtccctt cgattatagc ggtgtctatt atgccgccga
     5641 agccgaagat gcaatcgatg attctggaat atccgttgcc atatgccata tcgttctcaa
     5701 tgttccggat ctggataaag ctaggcattt ctatgaacag gccatgggat ttgccgtgcg
     5761 aagcgaaaaa aaaggcaagg cgcttcttct tacggccagc cagtgccatc accagatcgg
     5821 tttggccgca acgaaaaaga tttccaaagc cagcgcggtg ttttcgatct cctatccggt
     5881 atgcgaatcg ctgatgcaaa cgattgaaag aataaggggc aagggctttt tggtcaatga
     5941 aagcgaaaag ggatatctgg tgacggatcc gttcggaaac atgatctatc tggttttggg
     6001 ataatgaaca atatcataat taaaaaacgg ggaaaatccc cgttttgttt ttataacaaa
     6061 ccaagcgttc gataaataac gacaatgaga aagattgccg gaatcgagaa caaagaactc
     6121 cagaccacaa tttggccggc tagtttcccg tcgtttttca tctccgatgc cataatggcg
     6181 gaaactacgg ccaccgggct tccgaacgtg gcgatgagtg ccggataatc cgtgctttca
     6241 aatgcaaaga atgtcgtata tttcgccaaa atcaccgcca gagtgagcat aagtcccggc
     6301 gccatgatcg tccgcaaaag aactccgagg gtaatctccc ttttcatcgc ccggacttgg
     6361 gcgaactgga acccgccgcc aagcaccaca agcgccaaag gcgatgctat ctgtcctaac
     6421 catttgatac aggtatatag gaaagtcaaa tcattcttga gactgaaaac cggttcgccg
     6481 gtcgatgcaa atacaggaat cagcgatctt atataaagcg acaatatacc aagaactatt
     6541 gcaattatca gcggattcga aagtatcttc aacaaaacct ttatccaagg aactttttcc
     6601 ccgtcctcac ccctcaagaa gacggaaagc gcgatgactg acaaggcatt gtaaagcgga
     6661 atgatgacaa gcgatacaag cgccaccacc aaaaccgcat cggctccacc cagagcttcg
     6721 gccaaaggaa cgccgatgat cgtcatgttg gaccgaaaga ctccctggtt tatcacgcct
     6781 ttttgtgtcg gatccttgac aaaaaacatg gcgaccaaaa aaccgatcac aaacaagacc
     6841 aaaacaccgg cagaggcata caaaaccacg ttccatctta tgtcctgcaa tccggaagcc
     6901 gaatataccg tataaaacaa aagacaaggt aaggcgatcc ggaatacaaa acgattggca
     6961 caaggccaaa attcctcgcc cagaaaacgc gtgcgggaaa gaatatatcc gagaccgatc
     7021 attagtaata gtggcaatac agcattgagt gcaaataaaa gtgtgtccat cgatcatcat
     7081 ccgttatcca taattattaa caacatcaat tttaacatca tttggctttt gtgaaaagaa
     7141 aaaccgggaa tgatttcccg gttaatcctt atttcagcaa ttcttttttc tttttctcga
     7201 attcgtcctg cgtgatcgct ccggaatcat acaaatcttt gtactttttg atttcgtcgg
     7261 cggcactcac cgttttggtc gcaagaacgt tatcggtgga taccacaaca ccgagttcgt
     7321 atttatcccg gaattgttcg tccgtcatcg tcagaatcaa aataccttcg acaagaccga
     7381 tgatagccgg aatgccggtc cagcaaaaca ggacgtaaat taatcctaaa ccgacctgtc
     7441 ccagataaaa gcgatggaca ccgattccgc caagaaaaat cgcgaaaagt ccagctgcaa
     7501 ttctgttttt catgttattc ctccaattga tctatagtat ctttttgttt ctttaattcc
     7561 gctttgtttt gatacatctt tgcgtctgac aatttcacca acatgtccaa atcgcatttt
     7621 tcatcccggc aacagacaac tccgcatccg aaaaccactt gtatcgcatt accctggacc
     7681 ataaacggat tgtcgttgaa atagtctctg atctgtttga ttcgatcggt tgccttttct
     7741 tccgtcgcat tcgggaacac aaggaaaaac tcgtctcctc ccactcttga caaaaagaaa
     7801 ttgttgccgg cgttttttct aacgccatcg gcgaaatgga taagcaggtc gtctccagcc
     7861 tcgtgtccga aggaatcatt gacttccttg agattgttga tgtctatgga acatatcgcc
     7921 agtggggttt tattttctat tgcttcatta tatgccaatt tcaataattc ctggtgataa
     7981 ctacgggaac aggcatcggt aagataatca tatctgacac gtctgatggc atcgccgtac
     8041 aattcatgga tagtgatgat gatttcgatc tgtttggcga gttgtttgat catttgttta
     8101 tcgctttcgt cgaaaatgga atccgacaaa tcgctgtcaa gattgatcga gcccatgaat
     8161 ttcccattat gtttgaaaga acaagtcagc accgacattg cgcccagggc gttgccgctt
     8221 ctcaacaact tgatagtgtc cgcactgagt ttcttctcgt cgtatttttg caagtctcgg
     8281 attatcgtcg gttcgaacgg atcctcgaga tccgactgga acacgtcttt gtatttcaat
     8341 ttggttctct gtaaagtctt aaggtcataa ccgacagacg cccgatattc catcatttcc
     8401 ccgttaagaa tcaatatgct gccggaagac actttcggga ccgccttgac ggcttcttcc
     8461 agaatcatct gcaatacatc gtcgaaaaag tcggtattca gcatttccga tgttatcgtc
     8521 aacatcatct cggtgatccg atagtttagt tctatgtcat catatgcttt tcttaggcgc
     8581 cctaaagctt cctcttggct ggtgttttgc cttctcagaa tcgccgtcat tttccgttgc
     8641 ccaagatcag aacgagattg gacaagcgta aaaacatagg caagcactac cgacatcacg
     8701 ctaagacgta aattctcggt aaaaaaatgc tggaatcctt ccgttatcca tatcatcagg
     8761 ttaaacgcta tcagggcgta aagaaatacc gacagccgtt tccaaagcgg atataactgg
     8821 acaagcgaag tcgcaaacac cgcgatgatg aacgaataga tttgattgga ataaccctga
     8881 taagcaaacg aaatgatgat tccgaccgat actatcagat acggatgggc gtgcaccgtg
     8941 gctttcctaa cccatgaatc aaacgaaata gcaccggcat gaatcagcga aaaaagaacc
     9001 aactgcatga tcattagtcc ggtagtcaga agaaaataat atttggatct taacagcgac
     9061 ggatcaacgg caataaagga aatgatttca aaaatcgcta ttattgaagc cgatacgatc
     9121 cccatcgcca catcatagtg atcctgctcg ttttcatcgt agtaggttcc gaaaaatccg
     9181 gatattttgc gcaatacgct acgaaagaat ttcattgcta tctcctggat tataaatatt
     9241 tgttttgtta ttgcccaagt aaataacaga cacatgttat tatacaagat ttggtgttca
     9301 aagtaaataa gcggccgttt ctaattatta aagacggccg cttttttttt gcggccgtcc
     9361 ataattttcg ttgaatattc agtttactcg gcaagcgtcc acgccgcgta tagcgttata
     9421 tcctcgtaag gcatggtaga gaaggtgtaa ggaatcgtca aagccacatc ttcataccaa
     9481 ccatcaaaca catagccgtc cattgtcgga gccacgggtt cggtaactgc ggtaccttgt
     9541 tcctgccaaa tggaatcgac agcggttccg ccgttggatt caaaggcgat tgtcgtgttg
     9601 aaacagcccc atacgactgg cagtccgctg gaattccaac ttgcatccca tcctgccggc
     9661 ttgctggaag cttcgacatt gatcgtcaat ccggtacatc cgacaaaaac atctctgccc
     9721 attgcggtca cattgttttt aatcaagata gatgtcatcg acccgcaatt gtagaacgcg
     9781 ttgttaccga tagacgttat agtatcggga atgtaaaaat cagccaaact agtgcaaccc
     9841 ttgaatacat aatttccgat cgaagtcaaa tccccttcac cggcaaattg gaaacttacc
     9901 gatgtaaggc ttgtgcaatc ctggaatgca taatctccga gcgttgcgac agtcgccgga
     9961 atctcgaccg attcaagcgc gctacaggca ttgaaagtgt aagtgccgat ataaatcagg
    10021 ctggagcctt cttcaaacac gatattaacc aaatcgctac aatttaagaa cgcatagttg
    10081 taaatatgcg taaccgaagc cggaatcgtg atcgaagtca atgcggtaaa ggaaaaggcg
    10141 tattgtccta tatcctgcag ttgagatccg ggggcgaaaa tcacttctgt caaataagta
    10201 tcccagccga atgcgaaatc accgacagtc aaaacgttag acggaatggt gatagtggta
    10261 agggcgcatg actggaaagc atttgaaccg attgactgca actggctgtt ttccccgaaa
    10321 ttgaccgaag ctagcctgta atttccgtag aacgcatgtc cttcaattat cgtaacgcta
    10381 tccggaatcg tgatttcata cagattattg ttgctgaagg cgctttcccc aatctgattc
    10441 agattgctgc ttggagcaaa atagacatgt gtcaaggaat agcaatcgcc gaaggccata
    10501 tatccgatgt aagtaactga agccggaatc gtcacgctat gcaggttggt tgccgaaaag
    10561 gcttcatcac ctatttcttg caaaacactg ccgtcttcaa aaatcagttg tgccagttta
    10621 taacattgct caaaagcata tggttcgatt atcacaaccg ataccggtat gcttatcatt
    10681 ttaagattgc cgcaggcaaa gaaggcgcct tctttgattg tgacgatatt cgagttagac
    10741 gcaaagtaga catacgacaa atcatattgc tcataaaaag cgtatgaata aatcgaagtc
    10801 acaggatatg cggtaccgct tatggtgatc gcttccggta tagttatgct cgttacggtg
    10861 taagtgtcgg atccaagaag gattgcctcg ttgtttgaca atactacata agtgaggccg
    10921 ttttcggtcg tatagcccgc aatatcccat tcgaccgggt atccgccgtt gatccatccg
    10981 gcatcccaac cggccggtat tgtgctggct tcggcatata cggtcagata tgaattgtcg
    11041 taaaaagctt caaagccgat cgtttccacg ctggccggaa tgatgacgta attcaaatca
    11101 taagcattgt aaaacgcgtc ttctccgatt gatattaagc cgtcagggat atttatttgt
    11161 tccaaaccat aacattccgc gaaagcatat tcgccaatcg atattaccga atcgggaaga
    11221 ataacccggt tgatttcttc tgcgccgtaa aaggcattcg cttcaatgct tgtcgttcct
    11281 tccggaataa cgaccgttgt tctttcgaat gatgtcgaca acaagccgtc gctactgtaa
    11341 atcatgccgt caatattcgc aaccggcaga ttataggtgc attctgtcat atataaattg
    11401 ctgctactgg attcggattc ggaaaggatc agcgggttgt aataatcccc gatcgccttt
    11461 cccgacaaaa ccgaattatc gatttgaatt tggttttctc tgcattcggt catggaaaat
    11521 gttccttcat cgagatcgaa attgtcgtta taaatcatgc cgccatacaa ataagaatta
    11581 ttgatagtaa aatatccata ataagtgtat tcgagtgcaa tggcattcat tgcggttatt
    11641 gtcgtattgt taatgacgat gttggagttt tcatcaccgt aaaaatacat accgtataaa
    11701 gccgcgatat cacaattgtt catttccaag tttagtcctt ccggacattc catatacagg
    11761 aaactaaaca ttcctttgga agaattcacc ctgttcaaaa caagattaaa cggatatgtg
    11821 tagcctatat aaatcgcagt atcgtaactg gtgtcgttgt caagacccgg agccacctcg
    11881 agatatagat cgttgatctg gatgcttgat ccttctatta tagcgtattt gttataataa
    11941 tccctttcgc tgtcataatg actgccgata ctgatgaacc cgttggatcc ggcatatgag
    12001 taaagcgtgt agccgttacc gtaaatagat acctgtctgt cgatataaac ggaggacatg
    12061 gcataaatgt cggcgccaag ataaatctca tcatagctga aattgtctag agcggcagtc
    12121 aaactttcat aatcgtatac cgtaaccgac gtggctgttt gccatttggc ataaagaatc
    12181 agatcctctg tcaccaaagt attttcaaaa aactgcgaat tggaaacgcc tgttcccgtg
    12241 taccaaccaa ggaaactgta tccttctttt accggataag gcaaaatgat ggtcgttcca
    12301 taaggaactc cggcaatagc gttttccatg gcaccgccca tagtatcgaa cgcgacggta
    12361 aatgtctctg gtgaataggt tgccgttaca acggtgttac cggtaattga agaaaacgaa
    12421 tggtcccatc cggtgaaaac ataaccggca cgtacgggat ctgcaggcgc aactgctgcg
    12481 cttccgtatg tcaagtgctg tatatcaagt acggagccgt cgaaattctt gaattccacc
    12541 aaataagtaa ccaatatttg accgaggttc ttttccgtac cgtcggtata agtgacgata
    12601 agttctccct ggtcgttaat tgccattgag gaaatgccaa cgccggtttc tccggcggat
    12661 cccactagag aactgacttc aatcagattc aaccagctgg cgtctccgac atattgccat
    12721 tgaatatatc cgtcggatac tctaaacgtt acttcgcgtc cgtttgtccc gtcggcgccg
    12781 gtaataatcg caagggaaat caaatcagtc caggaaacat cactttcgta tttccattgg
    12841 atgtatccat cgaacacttg aaacattacc tcttccccgt ctgttccatc gattccatca
    12901 gctccgtcgg cgccaataag cgaagaaagt gcaatcagat tcgtccaggt cgtatctccc
    12961 acatattgcc actgaacata acccgaagcg acttgcaaat tgatgtcccg tccgtcttct
    13021 ccggggattc cctgttctcc ctgaatggat tccagccatt cttcataagt gcctggaaga
    13081 tctccggacg ccacaccaag atcatagatc tgatgcaatc taatgcttaa atcggattca
    13141 gtagtcgttg ttgtcaccgt aaaagcttcg gtggtcgtca aagtagtcac agcagtggtc
    13201 tcgtctttgc aggagactag aacaaacgcc atggcaatga tcgcgattgc caaaacgatt
    13261 ctttttttca tttttccgcc tcttttatat gtaagttatt tttataacta aatctcaaat
    13321 aaaaaaaccc aaaaccattt tgggcgtgtt tgggcttccg aaattaataa aaacaaatac
    13381 aacataatta tcgaaaattg tcttgccgcc acgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnntttatgg tagcccgtac gggattcgaa cccgtgaatg ttgccttgag
    13561 agggcaatga gttaaccatt tctccaacgg gccggattat cagtccggca acagactgat
    13621 agtatgatat attctttccc gaattttgtc aagtcccaaa atccgcagaa tatagtagaa
    13681 attcggggtg ttctttcttc cggacaccgc gactcggatt atttccgcaa agtctcccac
    13741 gtgaccgaca tattgttccg ggttcttttt ataatccttt ggcttttccg caaaattgca
    13801 tttagaagct gtggctttca aattgccaaa ccaatcttct tccgaaaggt caagtcccgg
    13861 attgtcaagg aattctttca gagatgtttt cacaagttca gtcgggaatc ggtcaattat
    13921 tgtcaatcct tcttttatag atgccaggaa tatatcagtg tacatgaatc cgatgaccgg
    13981 aaaaatgtca ctatatctcg tgtaatcttt tctgggtttt tccccacccc tttcgatggc
    14041 gagtatcgct tcgaaatatg tgggatcggt cgtgattctt tgatgtaatt catgatcgta
    14101 gatcttcgcc cattcatcgg ctttatcagc tatttcggaa gcgctcattg cgcccagacg
    14161 ttccttggcg atatggttga ttttttccag atcaaacaag gcgccatcta aagtcatctt
    14221 ggaaaagtcg agatggaagg aaaaaatatc agcctctgga ttctcgatcc gccactcctc
    14281 gaaattcgtg ttggcgattg tcattagata ctcgagaaag ccttcgaccg ggtaaccttt
    14341 ttcaaggaag taactgaccg aagcttcttc gtctttgcgt ttggacagtt ttctcctggt
    14401 ttccccatcc attttcatga taacggggag atgggcgtaa acaggccgtt tccagccgat
    14461 tgtatcaaac aattgcagat ggatcggaac cgacgacatc cattcttctc ccctagtcac
    14521 atgcgttgtc cgcatgaagt ggtcatcgac caaatgggca aaatgatagg tgggaagacc
    14581 gtcgcctttc atgatgacga tatcctggat gttttcggga aactcaatat gcccacgaat
    14641 ggcatcatcg aaagcaatcc ggttttcttc gttgccgggg gaacggaatc tgatgacata
    14701 actgttacct tttttgattc tttcagctgc ttcttccgct gtcaggaatc ggcacttagc
    14761 atattcgccg taataaccgg aattgagctt tttagcttct tgttccttcc tgatcgcatc
    14821 caattcctca tgtgaacaaa agcacggata ggcaagcccc cggctaatca aatatttaac
    14881 tacgatatca tatatccatt ttctttcgct ttggacataa ggtccgtaag cgccaccgct
    14941 caaaaaactt tcgtccggta cgattccgaa cgtaagcaat tcggccaaaa ccttatcggc
    15001 cgatccggat atctccctct tctggtccgt atcttccagc cggcagaaaa acaccccttc
    15061 gctttgcttg gcaaaacgcc aggaaaccat ggacgcgaaa agcgaaccgg tatgcaaaaa
    15121 accggtagga gacggagcga atctggtcac catcgcccct gacggcagtt ttctttcggg
    15181 gtatttagtt tcaagatcat ccaaagtcat cgagacattc ggaaaaatca aatcagccaa
    15241 tttatggttg gaatccatca cgcaaaccct tctttccgta acgaaaatat tttatcataa
    15301 aaacaaggga aaagccacat gtcataacgg caaatcgaat cccgtgacac tctctccggt
    15361 aaacggtacg actatatcca ccacttgaag attgctgtcg gtatagagca tatgcatttc
    15421 ggaatttgcc aaatcaatcg tggtagccat tccaatcagt ttttggccga acagattttc
    15481 cttaacgtac ggagtttgga catcgcttct tctggaaacg gtgaaagccg gaaaatcggc
    15541 gtcggtcata tatccgtgcg atttggttgt gtcatagtag gcttcgtcgt aatataatgt
    15601 ttcattaacc ggcataccgg gcaaaaactg catcagttgg tgcgtgtgtc cggacaatga
    15661 caaatcgata tcgattgtgt ttagcagatc agtccacaga ttcttgatgg tttccagaaa
    15721 gtaaatccca ctgccgaaat cggaagtatc gctgtagaca ttcgtgatcg gcatatggca
    15781 aatcaccaat ttgaacacta ctccggggtc gctgtaagcg tcttttgcga ttacatcgtt
    15841 aagaaattcg gtttgcatat ctcgatattc agtaaagtaa gcggtgtcgt aatattccca
    15901 ccaatcatcc gggtgatcct ctcccaaatc cagaacgatt ccaaaaatac cggagagatt
    15961 gaatgtgaaa taaaaagatt cattgacgct gccgacatat ttgtagagtt cgtcagccca
    16021 ttccccttta gtctcgtgat tgcctctggc ataaaccacc ggacgggttc cgccggtgat
    16081 gttgtaggcg atcctgtgga tgatcttgat atctgattct ttttctaaaa atgaggcgat
    16141 gtcaccggcc attaccaata agtcaaggct gtcgccgaaa taggttccgg tcttgctggc
    16201 ggctcccaca tattcatgag cgtctgctat tgtatatatc tgaattccgt ccgagagatc
    16261 gaccgggcgg aattcatagg tttccgtcac cgtgtgtccc tcaagtccgg aatacggtcc
    16321 ccgatataaa taattagtgg aaatgatctc gtattcgccg gcttgatcca aaacactcat
    16381 cggaacaaca accttgtgaa cggtcgtaaa cgaatcggca ctgcctgaat aagtatccac
    16441 gtatgtatca ttaccgattt tgaccaaacc ggtggcatcg atcgaagtcg tccagacgat
    16501 ctggtactcg tcttcgaccg cgaacacggt aggatgggtg gaaatcaaga caaaaccgga
    16561 aaaaaaggac accgctccca gaattaagat cattactgag atcaatacga tgcggaaaac
    16621 actgtccgcc cacaatttcg ttttaggaaa ataaaaaaac aggtagagga caaatgtggt
    16681 tccggccaga atcatcacat actcgatgaa gctcctaaaa atatagtgga acgatccgat
    16741 tcctagatat aacatgtata aaatggcaat accaagcgca atcgaaagtc cgccgggaat
    16801 ccacacccag ttctttttcg tttttataac catcggcatg aaaagcacca cggtcgcgcc
    16861 caaaatcaaa ccgagaatca cgtattgcaa ccaagatacg ccaaacattc cttcgatgcc
    16921 ggaccagatc gactgcatcg acatatataa aacaagggta aaaagcgata ctccgaataa
    16981 aatcctgttt ctcattttta tcatcgtcct tccatatgcg agcgatataa aaaggtgctc
    17041 gcaaaatgat ttacgctatg tttaaatagt atcatcattt ccattcccgc acaagagaat
    17101 atcgccctta taaagcaaaa aggcccgcca tcggcggaac ctttgtcgta attattatcc
    17161 tatctgatgt agatgtcgcc tgatacggtt ttcttgctga cgatttcgat cttgttgtat
    17221 tcagaattat gaatgttgat atcaccggaa accgaacgca aagtcacttt ttccggatag
    17281 aattcctttc cgtcgaaatc gccggaaacc gtatgataat ccaattcctt tactttggtg
    17341 tttttaacgg acacatcgcc agaaaccgta ccgatttcca tgtcattctc gatgtttgta
    17401 tccacgatct gaatatctcc gctgaccata gtggccgaca gtccggataa gcgggagttg
    17461 ttcaccttaa aatcgccgtt tactgaatgc cattctgtct cgccggcttc gacattgtcg
    17521 atcacgaaat cgccgtttgt cgtattgatg ctcagtttac cgattttgat gtttctgtat
    17581 tcgacatccg aattagttcc aacatgcttg aaattggcaa ctatgacact ttttggcaac
    17641 aacacccgga atttgattcc ttttcttgtt gtgttgaaat tgaaaatcat gtttttcctt
    17701 ttgtatgtca gtctcaaagt cttcccgtcg tattcggctt cgtagtcttc cggacgacgg
    17761 cattttgtcc agaacacctg gattgtttcg atatcggcag aggtatattc gatgtcatcg
    17821 ttgacaaacc tggtttcgac gtcgagcgta tccgactgta aagcgaattc cttccataat
    17881 gtcatcttta aagcgccttc ttcgtttatc tcttcttttt cgttttccaa atcctctgcc
    17941 aattggctag ccagtttgag cggatctccg aatttctttt caatgtcttc ttcggccagt
    18001 ccctcttcta tcgcgttggc gatcatttcc tcgtgatcga cgattatttc atcaatgatt
    18061 gaatcgacga ccttcttatt tttcaattct tgacgcaaat cttctagaaa tttcctcatt
    18121 ttttttacct ccttggttaa taatatggta gacgcctccg ataaaagcgt cccattcgtt
    18181 tcgaagttta agataatacc tgaatccctt gtctgtcatt tgataatatt tgcgcggagc
    18241 tccatcaggt gattcttcca aatatgtatc gaagtatttt tcagcggtca gccttcgcaa
    18301 aatcggataa atggtgtttt cattgacatc gatgtttttc gcgagctcgc tgattattct
    18361 atagccatac atatcctttt cggccaaaag cgaaagaacg cagagttcga ctattcctct
    18421 tttgaattgg gcattcatcc agccacctcc ttatagtgtt gtgtctaaca caatacctat
    18481 tgtcgcacaa gtgtttgtaa attgcaagtg ttttttataa ttaatttaaa atgatatcca
    18541 gaagccgttt ttttgatata ctattcatat accatatttc aggaaaggtc gaatacgtat
    18601 gattgacaaa agaatagcac tagcggaaaa aatggcctta cgcgatcctt ccaatatcaa
    18661 aacagtcatc gggtttgacg gcttcattga cgaaataatc catgttgtgg acaaaaggga
    18721 agacagcgaa cattttacaa gaatcggcaa aatcaacgat ctggccgaac gtatagcccg
    18781 ggcgggcggg ctttcaacca atattgaact gtttccgaca actcgcaagt tggggggaaa
    18841 cgggccgatc atggccaatg ctctttccat gcacaaatgt caccagacat atatcggcgc
    18901 tcttggcgtt cctgttattg acagtgtgtt cgcggaaatg gcgacaaaag ccgaggttta
    18961 ttctttgaca tccagcggac acaccgacgc tttggaattc gacgacggaa aacttttact
    19021 cggaaaaatg agttcgctcg gagatatcac ttatgacaga ctggttcttg ccgttggttt
    19081 tgacagattg gtgaaactgt tggaagaagc cgatcttttc gctacggtca actggtcgat
    19141 gattccgcat atgacggaac tctggaaaca cttgctcgag aatgtagtac cacgtttaag
    19201 cgataaaaag aaaaaaccgg tcttttttgt cgaccttgcc gatccggaaa aacgcaagga
    19261 cgaagagatc gctgaagctt tggaagtcat cgccggattc agaaaacatt attttgtcat
    19321 cctcggtctc aataagaaag aagcttatgg actggccggt tcgctcgggc ttttctcgtc
    19381 caagagcctt tcggaggatc tgtcattaaa ggaagtgaac caagctttgt atgactttat
    19441 cggaatcgac tgcgttgtga tccatccggt agaccgttct tctactgttg cgggaggtaa
    19501 tttttatgag gaattcggac cttatatcaa aaccccgaag ctttcaacag gagcgggaga
    19561 caatttcaat gccggtttcg ttctgggaat catgttggga ttttcacctg acgaggcttt
    19621 gttaatgggc atgggtacga gcgggtttta cgtccgcaag gcaaaaagcc cggattacgc
    19681 ggaacttacc ggttttctcc gagactgggc ggaaaacaaa atctgagttt gacaaaatca
    19741 aaaccgcagg gaagatccct gcggctttta tttttcttct tctgtcaatt ccttgacttc
    19801 ttcttcggtt attattctta cttccccatt cttggcatcc atgtaggtaa tcgccggtgg
    19861 cgatgattcc cgtagcgcct tcgatgttcg aaacaagcgg cagggagtat tccctagcaa
    19921 ccacggcccc gtgggaaagc gccgatccga cctcggtgat caatccagct aagaggcaat
    19981 agtatggcga ccagccgata tcggtgaacc cagcgaccat gatttcgcct ttttgcagtt
    20041 cgttggcgtc ttcaagcgac cttacgaccc tggctttacc aatggctata ccattgctga
    20101 cgggagttcc cttgaacagt ttggtactat gaatcttccg gttgagtaga atcggttcag
    20161 gttttccgat cgtaacatcg tcaaaatgca ataacatctg ttccggcaat aatcttcttc
    20221 tgacaagtgc ctgtttggca aaaatcgatg atttcttgtc gatcaattcc ccgatttcct
    20281 gatgagtgag gaaaaatatg cagtcttcgt ccggcaacaa gccctcttca accaatttgc
    20341 cggccaaatt acggtatgca tgtttgaact tatcgatcac tttgatgatc atcgatttgg
    20401 atttttcccg gtacctgaca ccgtcacgag aagaattgat tagatatcta aagattttct
    20461 tcttggcaaa tccgtaattt gccaggagca tatcgcgatt cttacgccat ccgttcatgt
    20521 ccgtctcggt atcgaaaacg ccgcctgcgg cgatattttt aaccatgccg gctagattaa
    20581 gaggctcgtc tctccaagaa cgacttctta attcggcttc tcgcaccgac ctgtgtccat
    20641 ggcgctccat gaaagatata taatcacgat tgatgtcgtt tgccggattt tcgaggaaag
    20701 aaagaatatc tttcttttcc atgccatcga catccggaaa ttgttttttc acctgtttgg
    20761 caatttttct cagcgacatc agaatatcga cgctctcgat tccgtcgata ttctccaaaa
    20821 ccgaagctac ggcggctctg gctttttcgg gactttcaaa atccggttcc aaagcggcgt
    20881 tcaaagcgct cagcatcgat ccggaataac atgacgagac atagtgataa aaaagcgaaa
    20941 gattcaactg ggtcagattg gcatcaatat tttcataaaa aatctttgtt ggcgcctgag
    21001 gatcaaatgt caaatcatcg tatagtttct cgtgtaagcg ggcatacttt tcctttttaa
    21061 ataacaatga tgcgtatctt acgaaattaa ccagtttgcg gataaatccg gctttttcca
    21121 gattgatatt ttcgctcgct tcgagagagc ggccgcagat tgaaatgtct acgccttgtt
    21181 tgtcggtcaa aatcatcatg ttttccatca tgtaaagcgt cgtcaggtca atgaacatat
    21241 gattgccgtt acttacgatg caactgccag gaggaatgtc gtccattctt ctgaaagttc
    21301 cggaaacgac caaaagtttc cgcatccccc attcgattcc atacatgctg gttgtaagag
    21361 tgagcggcgt caccgcacca ggcatcattt cactgatgtt gttggttgtg attacgcgct
    21421 tggtatagtc cttcacggta tccaactcat ggatagtggg agtgtccaag gtagttatcg
    21481 gtcttgcctg caaccagtaa agtcgtccgg ttttgctgac cacccattcg acatccagtt
    21541 cgcaaccaaa atatcccttg gctttttgac tgccgattct gatctcttcc aaatgggatt
    21601 catcaatgaa gcagtttacc ggtatgatcc attccgttcc gtcgtgacgg cactggatgg
    21661 cggaaacact tccggatacc aagttttctc cggctccttc caccgcttcg atcagagtca
    21721 tatggtcgtt taaaggatca attgtgaaag ccacaccgga tattttggca tctatgtatt
    21781 tttgaacgat gaccgtcatt tcagaaacct gacggttgag aaatatttcg ttatatttgc
    21841 gtacgcgttc gctgtcaagc gattctaggc aacggtgaaa agcttgaatg aagttatcct
    21901 ttcccgccac gttaagaacc gtatcatact gtccggcagc cgaactcgac cctccatcct
    21961 cggctgtcgc cgatgatctg acggcaaccg cgcccagatt ggatttttca taatattcgg
    22021 ccgcttccat gacatcggaa tcggatttta tatccgtgac tatgaaacct tcgggaaccg
    22081 cgagcccgat ttggctcaga taatacaatc cccgggcctt acctccgacg aaattaagca
    22141 aatctgcggg aattttgccg atatcgtata ttttcattaa aaaacctcat ttccgccagc
    22201 gtgattcgtc atcgttgaaa ccgaattcga tgattccccg ggccggtttc ccgttgattg
    22261 taaaattacc caagccttca tacaaatgat atttcccgcc gtcgaataca tagtcaaaaa
    22321 catcggttct ttcggccgtg atcacgtatt tttccttatc ggcggtttct acgatcgcct
    22381 taacggtatt ttgtcccaga ttgttattcc ggatgtcggt tctcatttca tggccgataa
    22441 cgcttttatg attaccaccg gcgatgtttg tgtttccgac ccatatttcg tcaagtgccg
    22501 ggtagctgac catgctgaag tttatggttt ttccgttttc atccagtgcc gccagccaca
    22561 tatgcatgtt catgtagtcc caactgcgtt tcccgaagga atggtcgcga atgcaaggcg
    22621 cggatatttt ctttttgact ccggatattg tgaatgttcc ttcaagtctt ccgccctgtt
    22681 cgtaatgagt ctgattattt tccgacaatt ctttgaaaaa ggcttttgtc catttggctt
    22741 tcgctatcgt cttagccatc ggacggggat ccatatcata ggaaaaatca aaaataggtt
    22801 catcgcttcg gaaaatgccg gaaaaagccg cttcgacatt ttttttggtt ttcgcatctt
    22861 caagaaaacc ggtaaattcc acctgccacg cactacccgg ttcaaggcat ctcactttta
    22921 gcggtatatc attttcaaac agttgctgct taagattata tctgttcttt ttgtcagcaa
    22981 aggcgaacca gacttcgtcg gcggatttgc cgccgcgttg tcccaaccgg caaaaaaggc
    23041 tcataccgga aagatcatgg gcggaaaaat aataggaatt gttgtattcg gacccggcat
    23101 catccggcaa gacgtatctt tcagcttctt ctctcgtata tttgcgattt ttacttcttt
    23161 ttttcaagga atggcagatc agggattcct tgatggcatc catgcatttc atatgagcaa
    23221 tattccctcc tcgttcgaaa ccaaatcgac agtttagacc gttttttcca aactatcact
    23281 attataccag cgacgggcat ttgtggcaac tttttccaat tctttcactt ttacattttt
    23341 ttcataaata tgaaaaaagc cggctatgcc ggcttctcta atcgattatt gccaaatgtt
    23401 gccattttct taccgaataa aaaaagcaat gtacttcggc gaagtgcacg gcattgcttt
    23461 ccctatttta tatggtgacc cgtgcgggat tcgaacccgc gaatgcatgc gtgaaaggca
    23521 tgtgagttaa ccgtttctcc aacgggccaa gtggcgcccc aactaggact tgaacctagg
    23581 accccatgat taacagtcat gtgctctaac caactgagct actgaggctt taaacaagaa
    23641 agcggtctgg cagcgaccta ctctcgcacg cgaacgtact accctc
//
LOCUS       DAOPJL010000021        21896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_2539_length_21896_cov_7.105490, whole genome shotgun sequence.
ACCESSION   DAOPJL010000021 DAOPJL010000000
VERSION     DAOPJL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21896
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_21896_cov_7.105490"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2315)
                     /locus_tag="PLP02_03910"
     CDS             complement(<1..>2315)
                     /locus_tag="PLP02_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HPF42623.1"
                     /translation="VAIMGKTGSGKSTLLNLLGSIDSPTDGHIEYMGDRLDRMNRRRI
                     NMYRQKIVAFVFQEYYLISSLTVYENIELALKLSDTDPEKIDETILEALSSVGLDAYS
                     TRMPGQLSGGEKQRVAIARAIAKNAQILLCDEPTGNLDKETATGIMDILKHISKNHLV
                     IVVSHDTELIKPYCDRIIRLDQGVIVEDTTLRATEAISMPADDPSFLAKKHSCFTLFG
                     LSFANIRKNIPVIIPLIIILVAAFTVFSCLFSISEYNYNDTFVNTLQYNDVYIVPVSE
                     YEENAIFVNDEMIMWGSQLYYENISTDDILRLQDKTSGLIPVYQSYFFNKSFQDFTYF
                     RFTYNPRFTMNCYVSLNFTEVVVVDDFSQFHQPLRYGRYPAKDNEVVIYDYMADMMIY
                     VDAYPEMETMEELLDNTLTDRDTGQSMKIVGILKSDYQRYEYTKDGSDSDYRFESRYL
                     AMLQVIFATADFIPQITSFGDTVSINNVIITDDTYDNTYDDDNEYRKIIVTDDISDLN
                     MLAVNPEMSVYIGILLSNYQVADILGIDVSEVDSDFFQNYGTINFSVYQYLNYYSWDK
                     TTLFGPRIQLYGIYESDSLNDAVLRCYSDFDWILGHKNGTLRKSYLSLSENWDLNSQI
                     LSSFHMPQKPQSFFEDNPDYYNSDFTEYTPFTILLMDTSQFMDSVKNTGTYLGIISAA
                     VMFIGICGYGCISIYRNRYRVGVIKSMGAANHSLMLIFSFETLFIITISYILSVISSS
                     YILRAINQDFNSDLPFDIIFFSLNVRQYLLV"
     assembly_gap    2316..2415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2474..3631
                     /gene="fliB"
                     /locus_tag="PLP02_03915"
     CDS             2474..3631
                     /gene="fliB"
                     /locus_tag="PLP02_03915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HPF42624.1"
                     /translation="MGLENILIAGNGTAAEFIVPDYYELFFCKGPECKNSCCSGWEVT
                     IPMKQYFLLQSMSAGKRAKTVMDKSFRLLPGAMPERYAEIVHDYETDDCLLHMKNGYC
                     LLHFEFGEKMLPAVCRYYPRGIRSRMAYECSLSNSCERTLELLFANNESLTFKTATLA
                     FAVEPVKDRGSNSEIDLYVRLRAFCFGILADRRYPFSVRVMKMGKFLDSLARDGSRNF
                     DDIDIDWIPKAPNIDKTLVVMAHVANSCKDVWDSLSDYSGKAMSYFGEKQGMNEYEEA
                     KRHLDSILPYNEIMFEKMLVNNLFFREFPYRKGFAGLDAEFVAFAGTYLFLRYLSLAL
                     MRDGSDIGDFVDIMAKVFRVVSHSAFERNMIIFLRQIDIERPESFQDMIGF"
     gene            3768..6083
                     /locus_tag="PLP02_03920"
     CDS             3768..6083
                     /locus_tag="PLP02_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42625.1"
                     /translation="MTFKKTGERNRGWASKIAPHPVLNIAALASVIGAIIAQILITPF
                     GVDTIVTSTIITITVALVCATITVLSITISLQRQEYFGVSLQEIRKLRQAPAFNFLTM
                     LLISVLITFGISLTFLLGFYVVMFDLIVCLFVYLSCFAIQELPLLIDSENQAKRILKR
                     QYRANRTNHTKTDMANINFAIENAIFTKSLFEAYQMFADKEADTDLMDDLLALQENHV
                     RNLVSSADKVKQIANMTMALQNVKSIMQEDSPFAGIMPDYRDYAFRMPRVLSLIHRIS
                     AKSGFSINMYDLFDMLVSELQDSNPSEEKKDLIYSTICNLVIDSLPEGDFWAIDILTK
                     RISYNPFALESSESVATLFDMLSLYIYYLCEKEEKLPKAIRNKIVSWINRPERIDDDI
                     RIRNWKQLFSIARESYNSDQSELYRLYWLCHWEFESFHEVKTYVMTGGFVARWYLAGY
                     LDSENIYADDFPTKLLEKTEYRKYVIDAIDDLFDDDGNINEESVDILPAFFETGGTYM
                     ESFMSDDRLKKQLLQVKTSIKQTDMEKRIDRIKDVDLKLIRDGLGKSMKEIISQYQGL
                     ARKLEIEDEDTEQKYFYVYAQRNKSDDYYETLGESFANGILNSIRDDILSETIVCDPD
                     RPKESLERIMSLGITHEYEHCLLNLAHDQNKTFQKEVRKFDSTLKHVSGHHLIPFDSV
                     LKKDSLAFNYEIDSLEVLPLDAQQTEEKADGYKRDDGQYIFEGMVVRRDKVIDFVEEE
                     YVTIKMVFRTRIVCHDIYRLDFWSKPKTISS"
     gene            6206..7111
                     /gene="trxB"
                     /locus_tag="PLP02_03925"
     CDS             6206..7111
                     /gene="trxB"
                     /locus_tag="PLP02_03925"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPF42626.1"
                     /translation="MEKTYDLVIIGAGPAGLAAAIYAKRAMLDFVVLEKWFPGGQIAN
                     TYEIENYPGIRKASGAELSDLMYAHATDLGSDIRMEGVESVRLNGDIKEVVSDKDTYL
                     AKTVIIATGASPRKLKIPGEDKFYGRGVSYCAVCDGALYKNKVVTVVGGGDVAAEDSL
                     YLSRMASEVYLVHRRDELRAVKTLQERIFAEPKIETIWNSVITEIIGRETLEAIIIRD
                     KTTGEEKLHKTDGLFVAIGMDPNTEFLSGELDLEDGWIVTDENCETVIPGVFAIGDVR
                     KKALRQVVTGVADGAIAVHFVTKYL"
     gene            7130..7408
                     /locus_tag="PLP02_03930"
     CDS             7130..7408
                     /locus_tag="PLP02_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42627.1"
                     /translation="MDFDTHKILADNACIKTSELLDKPELTKEEAVILVDFAHTARLH
                     YWFSGFDKNTPEMKENLKKANELVERAYRKLGLEKAATWFKESTSYDK"
     gene            complement(7424..7921)
                     /locus_tag="PLP02_03935"
     CDS             complement(7424..7921)
                     /locus_tag="PLP02_03935"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HPF42628.1"
                     /translation="MDAMYATHGFGPEVEPDSEILILGSFPSVKSREHGFFYMYPQNR
                     FWTVLSILFEDDFINTDIAKKRHLLKKYHIALYDVIESCTIKGSSDASITGVMPADIA
                     KIISNTQIRRIYLNGRKAYDLFNRYDRQFLKMATLLPSTSPANAAMRLEGLVDAWQII
                     KQKAP"
     gene            8008..8082
                     /locus_tag="PLP02_03940"
     tRNA            8008..8082
                     /locus_tag="PLP02_03940"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8040..8042,aa:Val,seq:cac)
     gene            8220..10058
                     /locus_tag="PLP02_03945"
     CDS             8220..10058
                     /locus_tag="PLP02_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPF42629.1"
                     /translation="MKKTAILILLAFVSFAFFACATDTSDTTTAIMTEDVRNDAINQE
                     YQTLRLSFPVALVADFVLPEASSADFAVSYSLDDVILTNRTITYVQEAYDREKTLEVS
                     ITYEGLSVTKQYVFMQVADPDLSEQEIIKLVFQACYDTILDDIPAKIYSDLTLPDMPL
                     DGVSVEYSSDISYVFHGRFIYTYPDIEETVLINATLRYGEEVRYVSTYVTMASIDELS
                     KIPAIYIYTEGGAAVESDEDYVSATMTVVTYEDNEAVTFLENAVLGIRLRGNSTLYMP
                     KKPYKIKFEEKTAMFTDYARKDWVLLANYADQTLVRNYLADELSRGVGMAFSPISVFV
                     DLYLNGEYQGNYMLTDQVEAASSRVDIEKESPNLDTGYLVELDKRLYDYDDWSLLDDN
                     WFLVEGIPFVIKEPDWDDDTYSIDQLYYIQDYFVTLLNTLKAKADYTDLIDEDTFIDW
                     FIIEEVMKNVDSGYSSVYFYKDAGGKLKMGPIWDFDLSSGNYGHLEPALRGPEGWYTS
                     RQDKNIFFYYLMQYSTFRSHLKVRWNELYETVILPLPDKIYETSDSIMLSRYQNFEMW
                     DVIGVDTSWYIAPEIHAIDTYEGQLWFLYDFLTQRIAWLNQNINNL"
     gene            10326..11483
                     /locus_tag="PLP02_03950"
     CDS             10326..11483
                     /locus_tag="PLP02_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42630.1"
                     /translation="MEKKPLIELVNVTKTYDEENVVNDLSLTIYENEFITLLGPSGCG
                     KTTTLRMIAGFIQPDEGKIIINGKIFNDLPPYARPIHTVFQRYALFPHLNVLENVAFG
                     LRNRSYESLEKFFGRDVLIEEAKKVSMDKEPSKHLVNKIINRKIEAMAKEALALVKLT
                     GFENRKTDMLSGGQQQRVALARALVDKPQILLLDEPLAALDLKLRQAMQYELKEMQKK
                     LGITFVFVTHDQEEAMTMSDRIVVMKDGVIQQEGSPKSIYNEPVNRYVANFIGETNII
                     PGIYVKKDLVHFLGTDFPCTGYTFANKEKVDVVIRPEDWDVVPLDKAKVVGEVTFSLF
                     KGVHNEYDVKVKNTTLLVHTYESYEVGQPIGLTIDPYEIHMMKVETDEQVI"
     gene            11467..12294
                     /locus_tag="PLP02_03955"
     CDS             11467..12294
                     /locus_tag="PLP02_03955"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF42631.1"
                     /translation="MNKSFRILATPYQIWLMLLVFVPLLAMIIISFTNLTSEVPLNEV
                     SFTLANFLILGETSTLVAFKNSLVYASLSTIICFVLGYFIAYKIKTSRFNNKFIVLTI
                     VILPMWSNLILRAEALMNVMSENNIIKSLIGFSILGNTAGTGFAVLFGLVFTYMPFMI
                     LPIYTTLEKIDPALEEAALDLGLTPFKKFWKVVSPLSFRGVVSGSIMVFLPTLAGFAI
                     PKIISRGQIIFIGNIIDTKFMYTSYDQGALLAVLILVFILGSILLLTRFDKEGETLL"
     gene            12291..13166
                     /locus_tag="PLP02_03960"
     CDS             12291..13166
                     /locus_tag="PLP02_03960"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF42632.1"
                     /translation="MNDKEYPVATLAEYGFRDYSRFRGILKVLSTILFYFIMVMLYLA
                     IIVIAVESVNSSRTMSSFASFSLEWYPNILQEEDLLLAIRNTFLVSTIATTLATIIGT
                     FSAIGLYAMEKKKRQKWMLMNNIPILNADIVTGFSLMLMFRLLMYIFPSIFGLFTLVM
                     AHLFFTIPYVILSVMPKLKETDPNLMDAALDLGVKPYKALVKVVIPAIKAGIFSGMLL
                     ALTMSIDDFVISYFNTGAGFDNLSIWIYAVIGRRNLTPSVYAFSTLLTLFTMIALLAY
                     QFIHKSKKIKRSVPK"
     gene            13163..14317
                     /locus_tag="PLP02_03965"
     CDS             13163..14317
                     /locus_tag="PLP02_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPF42633.1"
                     /translation="MKKFIIAFIALAVSLTFSGCTQTPKIRILNWGEYINDEVVANFE
                     REYGVNVVVDTADSNELFYSKIKSKTTAYDLVIPSDYMIEKMVSEGMLIELDYNKLPN
                     YVNITYMDGVEQILASMAETTYERNSTTIDYKNYAIPYFWGTFGIIYNNRVEGLEASL
                     EANGWDTYFDAANYFPTARRGMYDVAQFAYAASMFYLGENPNDYSPALLSASETAIRG
                     ADFSSWGDDILKRDVEADNLDMAFVYTGDYLDRLYIQLDEGKTMEEVQADFNIYIPET
                     TMVFADCMVIPYTAKNIDGAHQFINYLLDPDNMALNASVVGYCTTTEQAYDIVVAYLG
                     NAKTSLDNWAKAYLAYYNKDREGTYYPLTSLPSADIDAINTMIQNVIAGD"
     gene            14535..16421
                     /locus_tag="PLP02_03970"
     CDS             14535..16421
                     /locus_tag="PLP02_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42634.1"
                     /translation="MLKKILVWSLMFLFGTGIFVSQVTHVSAKGLSLIPGASADLINL
                     YETDGGGGTLEDSFKAATNTDTKLYSYMSESMGNNYTDEGDLSDNGIVSVIPKKVFYT
                     PGTYTYVGNEWGFYAKTTSENAWVYQCEVVIFDITVTLPGEFYGDSEAQVKVKPVIQC
                     VYHCTNNLNNLPGFDSSLDYVVSINPYNYFINNIYLANIVICSSVTEANSADSYLNTD
                     YVETMTIESISQEPNYAADSPLNKEMVFKIAMFVGSKLPLVGSYFSLISRSKTGMEIL
                     NGLLFCDKIIQGDIVDQTDKSIKIDISESYTTHIKYFEGWLQSVALGAYEGTENIEVG
                     DNADYNYLYNSLNGCFMKTTVEYTCDFPVDASGYKIELSSEMNFSTCISLDIVTQDES
                     TFELTKVAEVCGEADYGKYQRYGELMNMYEPTAVVINDPGTADYFKIAADYNGIYQIK
                     KTGCSTNTRIEIYDNVGNLVVSSTAVDFTFRVNSDIEYYVKTKYLSDDYTGTYNILVM
                     PQYMGLLSTDYYKYIYFTEPKAVTYYFCPTKSADYYIYTGGTQDTLIKLYDKYGNFIT
                     ANDDNINDDNAKILVHLVAGETYYVQLVNKSTTWGYAKVYVLECVYIDPDPFYIPKDG
                     IELM"
     gene            16597..17457
                     /locus_tag="PLP02_03975"
     CDS             16597..17457
                     /locus_tag="PLP02_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42635.1"
                     /translation="MTIGEKIAARRKEMGYSQAELAARLYVSDKTISKWETGRGKPEI
                     DTLKKISTVLGMTVDELLDDDMENQDTGNAKKSKRSFMEIISEKIPPLSYNKNDIAVV
                     WVLGIGLGILLDIILTWWFLRIVRIYGLDYPVGLLVLQVITTTMLIVAIAKAKTDLRF
                     LLAYMVFIVILLIYTLFSADRDFLEVLTILAEIAVPVLFILYIFGVVSERRIPLYIQL
                     VTIILAGITFLTRIFDFNLFANSSFTYVYNVINSFNFIITSIAFFFLLKGVLGPVSDM
                     PIQKEPSEAE"
     gene            17570..18802
                     /locus_tag="PLP02_03980"
     CDS             17570..18802
                     /locus_tag="PLP02_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF42636.1"
                     /translation="MQKKNITVIGGGASGVMAALAAAEDGASVTLLERNSRIGKKLLA
                     TGNGRCNYTNENLTADDYNHPDFVKHGLAQFSSTDTLVFFKNLGIDPKYEEEGKVFPR
                     SEQASSFLDIFLYELDRRGVNIVTDAYVQNIVAKKNGFLIFLADGTSYKADKVIISTG
                     GKAMPKSGSEGSGYVIAQSLGHTITDVFPALTKLKIDSPYLKQLDGVKFPGAVELFAD
                     NVSLQKEKDDILFTKYGISGPAIMQLSRKANEALAKKQKTEIKVSIIDTLSKRDIAKR
                     MDSSMDKPVDYSLIGLVNKRLIAALLKEAGVEKQNALVSSLRDDTRNKIIDLLFDWRF
                     PVKGSMGFDDAQVTAGGVSCEEIDPNTMESKLVKGLYFTGEIMDIDGKCGGYNLQWAW
                     TSGYLAGENAAKDMEYDD"
     gene            18792..20378
                     /locus_tag="PLP02_03985"
     CDS             18792..20378
                     /locus_tag="PLP02_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF42637.1"
                     /translation="MTIRITEIKLRPDEAIDKESELFHLKDKVSRKFRIVESDMFSFN
                     ITRKAIDARKKEDIFLVYSVDMELKNGKSLLAKGFPNVKAYEKPDINYPGKPKINPKY
                     RPVIVGFGPAGIFAALTLAKQGLSPLVLEQGPDVDKRTEAVSSFGKTGVFSPEGTVLF
                     GEGGAGTFSDGKLTTLINDPLCDIVLDTFIEHGADPEIKYQAKPHIGTDVLKGIIKSI
                     RKEIMDLGGEVRFNAKVTDFIIKGNKLEGIVVNDDENIEADMCILAIGHSARDTYELL
                     LDKGLKMEQKPFSVGVRIEHLQNAIDEAQYGKFAGHKALGPADYKLAYHAPSGRTAYT
                     FCMCPGGFVVNSSSEEGGIATNGMSYSKRSGVNANSALLVNVGPGDYGSDHPLAGIEF
                     QRGFERKANNLAGNKYYAPIQSLGNFLDDRITNEIGFVKPTYLPDTVFAQMADIYPDY
                     VIKTLKEALPVFDKKIKGFASNDAILTAPETRSSSPVRTTRSSEYETNIEGIYSIGEG
                     SGYSGGIMSSAIDGIRCQSK"
     gene            20406..21323
                     /locus_tag="PLP02_03990"
     CDS             20406..21323
                     /locus_tag="PLP02_03990"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPF42638.1"
                     /translation="MRLTTFIDKGKFYKANLHGHSTRSDGSLTQEAAVLAYKNKGYDF
                     LALTEHNVITKLDKYNTDSFILIPGMELHTYNKDITTIHHMVGLSDMDNDFIKDGTVL
                     ENIVYTENKTSLLALEKRLHDLGFLTIYCHPTWSHVNTSQFDFLEHTNMMEVCNYGTI
                     WEMSHNEDPTYWDDWLKQGKQIYGVANDDEHVIEQYFGGWNMVKCEEFSIKSLFESLK
                     KGSFYSTMGPMIEDFYVEDGIAHIECSNVKEITFVVHGWWGHTFRDKEGKLNKADFSL
                     PDRAYIVRAEITDLDGNVAWTNPIMLPRN"
     gene            21535..21822
                     /locus_tag="PLP02_03995"
     CDS             21535..21822
                     /locus_tag="PLP02_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF42639.1"
                     /translation="MASKGQKFKKVPLETKIKVVKEGLDEGKSGYYLGKKYGVSRKTV
                     ATWIRIYKRDGGLDIRKKGRPKADGQIDYKERYEILKKYQDYLGEVDREKK"
BASE COUNT         6438 a         4685 c         5038 g         5635 t
ORIGIN      
        1 aacaccagta aatattgtct gacattcaag gagaagaata tgatatcaaa aggcaaatcc
       61 gaattgaagt cttgattgat tgcacgaaga atataactag aggaaatgac agacaaaatg
      121 taggaaatag ttataatgaa taaagtttca aaagagaaaa tcaacataag actatggttt
      181 gcggcgccca tcgacttgat tacaccgacc cgataacgat ttctatatat ggatatgcac
      241 ccatatccgc atatgccgat aaacataacc gcagctgata ttattccgag atatgttccg
      301 gtgtttttaa cggaatccat gaactgtgag gtatccatta gcaaaattgt aaaaggagta
      361 tattccgtga aatcgctgtt ataataatcg ggattgtctt caaaaaagga ttggggcttt
      421 tggggcatat gaaaagaaga cagaatctga gaattcaaat cccagttttc ggaaagagac
      481 agatatgatt ttcttagtgt gccgttttta tgaccgagaa tccagtcaaa atccgaatag
      541 catcgcaata cggcgtcatt taagctgtca ctttcataaa taccgtacaa ttgtattcgt
      601 ggcccgaata aggttgtctt gtcccaactg taataattta aatattggta aacgctgaaa
      661 tttatggtgc cgtagttttg aaaaaagtct gaatcaactt cggaaacatc gattcccaga
      721 atatcagcaa cctgatagtt tgatagcaat ataccgatat ataccgacat ttcaggattc
      781 actgctagca tgtttaaatc agaaatatcg tcagtcacga ttatcttgcg atattcgttg
      841 tcatcatcat atgtgttgtc atatgtgtca tctgtgataa tgacattgtt tatcgaaact
      901 gtatcgccaa aagaagttat ctgcggaata aaatccgcag tggcgaaaat aacctgaagc
      961 atcgccaggt atctggattc gaagcgataa tcgctgtcgc taccgtcttt cgtatattcg
     1021 taacgctgat aatcgctttt cagaataccg acgattttca ttgattgacc ggtgtccctg
     1081 tcagtaagtg tgttgtccaa taattcttcc attgtttcca tttccggata tgcatctaca
     1141 tagatcatca tgtcagccat ataatcgtat atgacgactt cgttgtcttt ggctggatat
     1201 ctgccataac gaagcggttg atggaattgg gaaaaatcat cgacgacaac gacttccgtg
     1261 aaattcagcg acacgtaaca gttcatcgtg aatctaggat tgtatgtaaa gcggaaatac
     1321 gtgaaatcct ggaatgattt attaaagaag tatgattgat aaacgggaat caaacccgac
     1381 gttttgtctt gtaaacgcag aatatcgtcc gttgatatgt tttcataata gagttggctt
     1441 ccccacataa tcatttcatc attgacgaaa atggcatttt cttcgtattc gcttaccgga
     1501 acgatataaa catcattgta ttgcaaagta ttgacaaatg tatcgttata attatattcg
     1561 gaaatggaaa aaagacatga aaagacagta aaagcagcaa ccaatatgat aatcaagggg
     1621 attatgacgg gaatattttt gcgaatattg gcaaaagaaa gaccgaatag cgtaaaacaa
     1681 gaatgttttt ttgccaaaaa agacggatca tcggcaggca tagaaatcgc ttcggttgct
     1741 cgtaaagtcg tgtcttcgac gattacgccc tgatccagac gaataatccg gtcgcaatat
     1801 ggttttatta gttcggtgtc gtgggaaaca acaatgacta agtgattttt ggaaatatgt
     1861 tttaaaatat ccatgatgcc ggtagcggtt tctttatcca gattgcctgt cggttcgtcg
     1921 cataatagga tctgtgcgtt cttcgctatg gctctggcga tagctactct ttgtttctct
     1981 cctccgctta actgtccggg cattcttgtg gaataggcgt ccaatcccac ggaagacaag
     2041 gcttcaagga ttgtttcatc gattttttcc gggtctgtat cagacagctt gagcgccaat
     2101 tcaatgtttt cgtatacggt aagggaactt atgagatagt attcttgaaa aacaaaagcg
     2161 acaattttct gccgatacat attgatccgg cgtcgattca tccggtccaa acggtcgccc
     2221 atgtattcta tatgaccgtc tgtagggctg tctatgcttc ccagcaggtt aagcaaagtc
     2281 gattttccgc tgccagtttt tcccatgatt gccacnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnaaatc catgatataa ttctgtctca ggaacaaatg ataaggccga
     2461 tggagatgct atattgggtc ttgagaatat cttaattgcc ggcaacggga cagccgccga
     2521 attcattgtg ccggactatt acgaattgtt tttctgcaaa ggtccggaat gtaaaaattc
     2581 atgctgcagc ggttgggaag tgacgatccc gatgaagcag tattttttat tgcaaagcat
     2641 gagtgccggc aaaagggcga aaaccgtaat ggacaaatct ttcagattgt tgcctggcgc
     2701 aatgcctgag cgatatgccg aaatcgtcca tgactatgaa accgacgact gtctgttgca
     2761 tatgaaaaac ggttattgtc tgcttcactt tgaattcggg gaaaaaatgc taccggcggt
     2821 atgccgttat tatccccggg ggattaggag caggatggct tacgagtgtt cgctgtcaaa
     2881 cagctgcgaa cggacgttgg agctattgtt cgcaaacaat gaatcgctga ctttcaaaac
     2941 cgcaacgctc gcttttgccg tagagccggt aaaagacagg ggttcgaata gcgaaataga
     3001 tctttacgtc cgtttgcggg cgttttgttt tggcattctt gcagatcgca gatatccttt
     3061 ttctgtcagg gtcatgaaaa tgggaaagtt ccttgattca ctggcaaggg atggttcccg
     3121 taactttgac gatatcgaca tagactggat tccaaaagcg ccaaatatcg ataaaaccct
     3181 tgttgtcatg gcccatgtcg ccaactcatg caaggatgta tgggattcac tgtccgatta
     3241 ttcaggcaag gctatgagct actttggcga aaagcaggga atgaatgaat acgaagaagc
     3301 gaagagacat ctagacagca tacttccata taacgaaatc atgttcgaga agatgctcgt
     3361 caacaatctg ttcttccgcg agtttccgta tcgaaaaggc tttgccggtt tggatgcgga
     3421 attcgtggcg tttgccggaa cctatctttt tctacgctat ctgtcgctgg ctttgatgag
     3481 ggatggaagc gatatcggcg atttcgtgga tatcatggct aaagtgttcc gggtcgtctc
     3541 ccatagcgct ttcgaacgta acatgattat ttttctcaga cagatcgaca ttgagagacc
     3601 ggaaagtttt caagacatga tcggattctg atgatatcgt aaaagacctc caaaaggagg
     3661 tttcttttat ctttggaaat gcatgaggcc ttattgcttt ttgatttccg ccattataaa
     3721 ataaaaacag gcaaaatcct tttttaggcc ggaaacgggg taaatcgatg actttcaaaa
     3781 aaacggggga aagaaaccgc ggatgggcaa gcaagatagc gccgcatccg gttttgaaca
     3841 tcgccgcctt ggcgagcgtg atcggcgcga tcatcgccca gattctgatt acgccttttg
     3901 gtgtcgacac gatcgtcacc agcacgatca ttacgatcac cgtggcgctg gtctgcgcca
     3961 ccatcacggt tctatccatc acgatatccc tgcaaagaca ggaatatttc ggtgtatctt
     4021 tacaggaaat caggaaactg agacaagccc cggccttcaa ctttctgacc atgcttctga
     4081 tttcggttct tatcactttc ggcatcagcc tgacattcct tctgggattt tacgtcgtga
     4141 tgttcgatct tatcgtttgt ctgttcgttt atctgtcctg ctttgcaatc caggaacttc
     4201 cgcttttgat cgatagcgaa aaccaagcaa aaagaatact taagaggcag taccgggcca
     4261 accggaccaa ccataccaag acagatatgg cgaacatcaa tttcgcgatt gaaaacgcca
     4321 ttttcaccaa aagcctgttc gaagcctatc agatgtttgc ggacaaggaa gccgacaccg
     4381 atctgatgga cgatcttctg gcattgcagg aaaaccatgt ccgcaatctg gtttcaagcg
     4441 ccgataaggt gaaacagatc gccaacatga cgatggcact gcaaaacgtc aaatcgatca
     4501 tgcaggaaga ttccccgttt gcggggatca tgccagatta ccgggactac gctttccgga
     4561 tgccgcgggt tctttccctg attcacagga tttccgccaa atccgggttt tccatcaaca
     4621 tgtacgatct tttcgacatg ttggtgtccg aactccagga ttccaatcct tcggaggaaa
     4681 agaaggatct gatttattcg accatttgca atctggtgat cgattccctg ccggaaggcg
     4741 atttctgggc tatcgacatt ctgaccaaaa gaatttctta caatcctttt gccttggaat
     4801 ccagcgaaag tgtcgcgact ctttttgata tgttaagcct gtacatctat tacctttgcg
     4861 aaaaagaaga aaagcttccg aaagctatca ggaacaagat cgtttcctgg atcaaccgtc
     4921 cggaaagaat cgacgacgac atcaggatca gaaactggaa acaactcttc tctatcgcca
     4981 gggagagtta caattccgat caatcggaac tctaccggct atattggctt tgccactggg
     5041 aattcgaatc gttccacgag gtaaaaacct atgtcatgac aggtggattc gtcgccagat
     5101 ggtaccttgc cgggtatctc gattccgaaa acatctatgc ggatgatttt ccaacaaaat
     5161 tgttggaaaa gaccgaatac cggaaatacg tcatcgatgc gatcgacgac ctgtttgatg
     5221 atgatggtaa cattaatgaa gaatctgtcg acatactgcc ggcattcttc gaaaccggcg
     5281 gaacttacat ggaatcattc atgagcgacg atcgtctgaa aaaacaattg cttcaagtga
     5341 agaccagcat caaacagacc gatatggaaa aaaggatcga ccggatcaag gatgtcgatc
     5401 tgaaactgat cagagacgga ctcggaaaat ccatgaaaga gattatttcg caatatcagg
     5461 gacttgctcg caagctggaa atagaagacg aggacacgga acagaaatac ttctatgtct
     5521 atgcccagag aaataaatcc gatgattatt atgaaacatt aggcgaaagc tttgcgaacg
     5581 gcatcctgaa ttcgatcagg gatgacatct tatccgaaac gatcgtctgc gatcccgacc
     5641 gtccgaagga aagccttgaa aggatcatgt cgctcggtat cacccacgaa tacgaacatt
     5701 gtcttttgaa tctcgcccat gaccagaaca agaccttcca gaaagaagtc aggaaattcg
     5761 attcgacatt gaagcatgtt tccggacatc atctgattcc gttcgactct gtgcttaaaa
     5821 aggattccct tgctttcaat tacgaaatcg attccctgga agttttgcct ttggatgcac
     5881 aacagacaga agaaaaagcc gacggctaca aacgtgacga cggccagtac atcttcgaag
     5941 gcatggttgt gagaagagac aaagtcatcg acttcgtcga agaggaatac gtgacgatca
     6001 agatggtctt caggaccagg atcgtttgtc acgatattta caggctcgat ttctggagca
     6061 agcccaaaac catcagttcc tgatttgcat ctatccggca aggactttat atcctgccgg
     6121 ttttttttct gaaaaaccgg tattcttgaa aaaagccggg aaattacgta taattgaata
     6181 agagtggaat atccggagga acaaaatgga aaaaacatat gatctcgtga ttatcggcgc
     6241 cggtccggcc ggactggcag ccgccatcta tgccaaacga gccatgctcg atttcgtggt
     6301 cttggaaaaa tggtttcccg gcggacagat cgccaacact tatgaaattg aaaactatcc
     6361 cggaatccgc aaagcgtcgg gagccgaact ttccgatctc atgtatgccc atgcgaccga
     6421 tcttggcagc gatatccgga tggaaggcgt ggaaagcgtc cgtttaaacg gagatataaa
     6481 ggaagttgtc tccgacaagg atacttatct cgcgaagaca gtaatcattg ccaccggagc
     6541 ttccccgcgg aagctgaaaa tccccggaga ggataagttc tacggccgcg gagtatcata
     6601 ttgcgccgtc tgtgacggag ccttgtataa gaacaaagtg gtcaccgtgg tcggtggcgg
     6661 cgatgtcgcc gccgaggatt ccctgtactt gtccagaatg gcgagcgaag tctatctcgt
     6721 tcaccggcgc gacgaactca gggcggtcaa gacgcttcag gaaaggatat tcgccgagcc
     6781 gaagatcgaa acaatctgga attcggtgat aaccgaaatc atcggccggg aaaccctgga
     6841 agcgatcatc atcagggaca aaaccacggg agaggaaaaa ttgcataaga ccgacggcct
     6901 tttcgtggcg atcggaatgg acccgaatac ggagtttttg tccggcgaac tggatcttga
     6961 ggacgggtgg atcgtgacag acgaaaactg cgaaaccgtc attcccggag tatttgcgat
     7021 cggggatgtc agaaagaagg cattgagaca agtggtaacc ggcgtggccg acggagcgat
     7081 agccgtccat tttgtcacaa aatatctata acagaaggag tggtagactg tggattttga
     7141 cacacacaaa attttggcag acaacgcttg tatcaaaaca tccgaactat tggataaacc
     7201 ggagctcaca aaggaggaag cagttatcct tgtggatttc gcccataccg ccaggttgca
     7261 ttactggttc agcggttttg acaagaacac cccggaaatg aaagagaatc tgaaaaaagc
     7321 gaatgaactc gtggaaaggg cttatcgcaa acttggcctt gaaaaggccg cgacatggtt
     7381 taaagaatcg acttcatacg acaagtaaga acaaaaaagc gcctcagggc gctttttgtt
     7441 tgatgatttg ccaggcgtcg acgagccctt ccaatcgcat ggcggcgttg gcgggacttg
     7501 tcgagggaag cagggttgcc atttttaaaa actgcctgtc gtatctgtta aacagatcgt
     7561 atgccttgcg accgttcagg taaatgcgcc tgatctgggt gttactgatg attttggcga
     7621 tatctgcagg catcacaccg gttatcgaag catcggaaga tcctttgatg gtgcaggatt
     7681 cgatgacatc ataaagcgca atgtgatatt tcttaagaag atgtctcttt tttgcgatgt
     7741 ccgtgttgat gaaatcgtct tcgaacagga ttgacagaac tgtccagaaa cggttttgcg
     7801 gatacatata gaaaaaaccg tgttcacggc ttttgacgga aggaaaactt ccgagtatca
     7861 ggatttcgct atccggttcg acctcggggc cgaagccgtg cgtggcatac atcgcgtcca
     7921 tgtcatcagt ataacacaaa agaatactag ccgtaacaag tttatgttgg cgaaaccgaa
     7981 acgtttatga tataatcttt tctggacgga gtcatagctc agcgggagag cacttgcttc
     8041 acgagcaagg ggtcgtgggt tcgaaaccct ctgactccac catcagtttt ttgacggttt
     8101 tcttttgaaa accgtttttt ttatcgtaca gctttcttaa atgatttcca tgttttgcga
     8161 attatgtgaa aataagcgga caatgtgata aaatatgttt aacaaaaagg aagatgaata
     8221 tgaaaaaaac agccatactg atattgcttg catttgtttc ctttgctttc tttgcctgcg
     8281 cgaccgatac ctcggatacg acaaccgcca ttatgacgga agatgtaaga aatgatgcga
     8341 tcaatcagga atatcaaacc ctgagattgt cttttccggt cgccttggtt gcggactttg
     8401 tgcttcccga agcttcttcg gccgatttcg cggtttctta ctcgcttgac gatgtgattt
     8461 tgacaaaccg gacgatcact tatgtccagg aagcctatga ccgggaaaag acgttggaag
     8521 tttcaatcac ctacgaaggc ttgtcggtga cgaaacagta tgtcttcatg caagttgccg
     8581 atcccgactt gtcggaacag gaaatcatca aattggtttt ccaggcttgt tacgatacga
     8641 tacttgacga tattcccgca aaaatctatt cggatctgac actgccggat atgcctcttg
     8701 acggtgttag tgtcgaatat tcgtccgaca tatcctatgt tttccatgga aggttcatct
     8761 acacatatcc ggatatagaa gaaaccgtcc tgatcaacgc tactttacgc tacggcgaag
     8821 aagtccgata tgttagcact tatgtaacga tggcttcaat agacgaatta agcaagattc
     8881 ccgccatcta tatctatacc gaaggcggag ccgctgtgga atcagacgag gattatgtaa
     8941 gtgcgacgat gactgtcgtc acatacgaag ataatgaagc tgtgactttc ttagaaaatg
     9001 ccgtgttagg aataaggtta agagggaatt cgactctcta catgcccaaa aagccttata
     9061 agatcaaatt tgaggaaaaa acggccatgt ttactgatta tgcgagaaaa gattgggtat
     9121 tgctggcgaa ctatgccgat cagactctgg tcagaaatta tctggctgac gaattgtctc
     9181 gaggcgtcgg aatggctttc tcgccgatct cggttttcgt cgatctttat ttaaatgggg
     9241 aatatcaggg caattacatg ttgacagacc aagtggaagc ggcttcttcc cgggtcgata
     9301 tcgaaaagga atccccgaat ctcgataccg gatacttggt ggaactcgac aagcgtcttt
     9361 atgattatga tgattggagt ttattggatg ataactggtt tcttgttgaa ggtattccct
     9421 tcgtgattaa agaaccggat tgggacgacg atacctattc catcgaccaa ctttattaca
     9481 tccaggatta ttttgttacc ttgcttaata ctttgaaggc gaaagcagat tacaccgatc
     9541 tgatcgacga agacactttc atcgactggt tcatcatcga agaagtgatg aaaaacgttg
     9601 attccggtta ttcgagcgtg tatttctaca aagatgcggg cggaaagctg aaaatgggtc
     9661 ccatctggga ttttgacctg tcctccggta actacgggca tttggaaccg gcgcttcgcg
     9721 gtcccgaagg ctggtatact tccagacagg acaagaatat ctttttctat tatttgatgc
     9781 agtattccac atttagaagt catttgaagg ttagatggaa cgaactatac gaaacggtga
     9841 ttctgccgct tcctgacaag atttacgaaa cttccgattc aataatgctt tcgcgttatc
     9901 aaaactttga aatgtgggac gtgatcggtg tagacacatc ttggtacatc gctcccgaaa
     9961 tccatgcgat cgacacctac gagggacagc tctggttcct gtatgatttc ctgacccaga
    10021 ggattgcctg gctcaatcaa aacatcaaca atctctaaag actgaaaaag cccttatccg
    10081 gtttaatccg aagggctttt ttttcgttta tcccaaccgc tggaaaaacg aatggcaaaa
    10141 taacggaaaa aggccgtttt aggccatatt ttgaaatcaa ggataaccgc caagtctcgc
    10201 tgtttaaacg gatttgcaaa tttgcaggca cgaatatgaa aaaaaactga aaaaatatct
    10261 tgcatttaca aaaaaatgcc atataattat tgcatgtaaa acaaatattg tccagggggc
    10321 cgcaagtgga gaaaaaaccg cttatcgaac tcgtcaatgt gacgaaaact tatgacgaag
    10381 aaaacgttgt aaacgacctc agcctaacca tctacgagaa cgaattcatc acgcttctcg
    10441 gaccatcggg gtgcggaaaa accacgactc tgaggatgat tgccgggttc attcagccag
    10501 atgaaggaaa aatcatcatc aatggcaaga tcttcaacga tcttcctcct tatgccagac
    10561 cgatccatac tgttttccaa cgctatgcgc tttttccgca tctaaacgtt ttggagaacg
    10621 ttgctttcgg attgagaaac cgttcttacg aatccctgga gaaatttttc ggcagggatg
    10681 ttttaatcga ggaagcaaaa aaagtttcca tggacaagga accttcaaaa catcttgtca
    10741 acaagattat caaccggaaa atcgaagcga tggcgaaaga agctttggcg ctcgtcaaat
    10801 tgaccggatt cgaaaatcgc aagaccgaca tgctttcggg cggccagcaa caacgcgtgg
    10861 ccctggcgag agcgctggtc gacaaaccgc aaattctgct gcttgacgag ccgttggcgg
    10921 cgctcgattt aaaattgcgc caggccatgc agtacgaact gaaggaaatg cagaagaaac
    10981 tgggaatcac tttcgttttc gtcacgcacg atcaggaaga agcgatgacg atgagcgacc
    11041 ggatcgtcgt catgaaagac ggtgtcatcc aacaggaagg ttccccgaaa tcgatctaca
    11101 acgaaccggt aaaccggtac gtcgccaatt tcatcgggga aacgaacatc atcccaggaa
    11161 tctatgtcaa gaaagaccta gtacatttcc tggggaccga ttttccctgt accggatata
    11221 cattcgccaa caaggagaaa gtcgatgtcg ttattcgtcc ggaagactgg gacgtcgtcc
    11281 cgcttgacaa ggccaaagtg gtgggagaag tcaccttcag cctgttcaag ggcgtccata
    11341 acgaatacga cgtcaaggtc aaaaacacca ctttgttggt ccatacctat gaaagttatg
    11401 aagtcggaca gccgatcggg ctgacgatcg acccttatga aatccatatg atgaaggtcg
    11461 aaacggatga acaagtcatt taggatcctg gccacgccat atcagatatg gctgatgctt
    11521 ctggtttttg tgccgcttct tgcgatgata atcatcagtt tcacgaacct gacatcggaa
    11581 gtgcctttaa atgaagtgag tttcaccttg gcgaattttc ttatcctggg cgagacttcc
    11641 acattggtgg ctttcaagaa cagtctggta tacgcaagtc tttccaccat catctgtttc
    11701 gttctaggct acttcattgc ttacaaaatc aaaacctccc gcttcaacaa caaattcatc
    11761 gttctgacga tcgtcatcct gccgatgtgg tcgaacctga tcctccgcgc cgaagcgttg
    11821 atgaatgtca tgagcgaaaa caatattatc aagagcttga tcggcttcag tatcttgggg
    11881 aataccgccg ggaccggatt cgctgtcctg ttcggacttg tcttcaccta tatgcctttc
    11941 atgatccttc cgatctatac gaccctggag aagatcgatc ccgctttgga agaagccgct
    12001 ctcgatctcg gactcactcc cttcaaaaaa ttctggaagg tggtttctcc tctgtctttc
    12061 cggggcgtcg tttccggatc gatcatggtg tttctgccca cgctggcggg atttgcgatt
    12121 ccgaagatca tcagccgggg gcagatcatc ttcatcggga acatcatcga taccaaattc
    12181 atgtacacga gttatgacca aggagccctg ctcgcagtcc tgattctggt cttcattctc
    12241 ggttccatcc ttctgcttac aagattcgac aaggaaggag agaccctcct atgaatgaca
    12301 aagaatatcc agtcgccacc ctggccgaat acggattccg ggattattcc cgcttccggg
    12361 gcatattgaa ggttttatcc accattctgt tttatttcat catggtgatg ctgtaccttg
    12421 cgatcatcgt gatcgccgtc gagagcgtca attccagccg gacgatgtcg tcatttgcct
    12481 cgttctcgct agagtggtat ccgaatatct tacaagaaga agacttgttg ctggcgatca
    12541 ggaacacctt ccttgtatca acgatagcta cgacgcttgc gaccatcatc ggcacttttt
    12601 ccgccatcgg attgtatgcg atggaaaaga agaaaagaca gaaatggatg cttatgaaca
    12661 acatcccgat cctgaatgcc gatatcgtca ccggattttc cctcatgctg atgttcagat
    12721 tgctgatgta cattttcccg tccattttcg gacttttcac tcttgtgatg gcgcatttgt
    12781 tcttcacgat tccctatgtc attctttccg tgatgccgaa actgaaagag accgatccaa
    12841 acttgatgga tgccgccctt gatcttggcg tcaaacccta taaagcgctt gtcaaggtcg
    12901 tcattccggc gatcaaagcc ggaatcttct ccggtatgct cctggcgctg acgatgtcga
    12961 tagacgactt cgtcatcagt tatttcaaca ccggcgccgg cttcgacaat ctgtcgatct
    13021 ggatatacgc cgtcatcgga agacggaacc tgacgccttc ggtttacgcc ttctcgaccc
    13081 tgctaaccct gttcaccatg attgccttgc tggcctacca gttcattcac aaatccaaaa
    13141 aaatcaaaag gagcgtccca aaatgaaaaa attcatcatc gctttcatcg cattagcagt
    13201 ttcattgaca ttttccggat gcactcagac tcccaaaatc aggatcctga actggggcga
    13261 gtacatcaac gatgaagttg tcgccaattt cgaaagggaa tacggagtca acgttgtcgt
    13321 cgacaccgcc gattcgaatg aattgttcta ttccaagatc aaaagcaaaa caacggcata
    13381 cgatctcgtg attccgtcgg actacatgat cgagaaaatg gtttcggaag gcatgcttat
    13441 agaacttgac tacaacaaat tgccaaacta tgtgaatatc acctatatgg acggagtcga
    13501 acagattttg gcttcgatgg ccgagaccac ctatgaaaga aactccacca ccatcgatta
    13561 caagaattac gccattcctt atttctgggg aaccttcggt atcatctaca acaatcgcgt
    13621 ggaaggcttg gaagcatcgc tagaagccaa cggctgggat acctatttcg acgctgcgaa
    13681 ttatttcccg acagccagaa gaggcatgta tgatgtagcc cagttcgcct atgcggcctc
    13741 gatgttctat ttaggggaaa accccaatga ttacagcccc gcgttgctta gcgcatcgga
    13801 aacagccatc cggggagccg atttctcatc ctggggcgac gatattttga aacgcgacgt
    13861 ggaagccgac aatctcgata tggcgtttgt ttataccgga gattacctcg acagactgta
    13921 tatccagctt gacgaaggaa agacgatgga agaagtccaa gccgatttca atatctacat
    13981 tcccgaaaca acgatggttt tcgccgattg catggtcatt ccctatacgg cgaaaaatat
    14041 cgacggcgcg catcagttca tcaattattt gcttgatccg gacaacatgg cgcttaacgc
    14101 ttcggtcgta ggctattgca cgacgaccga acaagcatac gatatcgtcg tcgcctattt
    14161 gggaaatgcc aaaacctccc ttgacaactg ggctaaggct tatcttgcct attacaacaa
    14221 ggatcgcgaa gggacatatt atccgctcac atccctgcct tcggcggata tcgacgcaat
    14281 caacacgatg atccagaatg tcatcgccgg cgactaagta aaaaaattat agaactaaag
    14341 atcggttctt cattatgaag agccgatttt tttaataaat gttgccgatg atgtatgaaa
    14401 acattaaaaa agatgtaaat cctgatgata tattttaaaa aactacgaca tttccgccct
    14461 gttttaataa tacgtaatgt gaaataatgt tattatcaat agatttataa attcaataaa
    14521 aggagagtgt tacagtgttg aagaagattt tggtttggtc attgatgttt ttgtttggaa
    14581 caggcatttt tgtaagccaa gtcacacatg tatctgctaa agggctttcc ttaattcccg
    14641 gtgcaagcgc ggatttgatt aatctttatg aaaccgatgg aggcggaggg acattggaag
    14701 acagtttcaa agcagcaaca aacacggaca ccaaattata cagttatatg tcagaaagca
    14761 tgggaaataa ttatacggat gaaggcgact tgtctgataa cggcatcgta tccgtaatcc
    14821 cgaaaaaagt attctatacc ccaggaacat acacatatgt gggcaacgaa tggggatttt
    14881 acgcaaaaac gacaagcgaa aatgcttggg tataccaatg cgaagtggtg attttcgata
    14941 ttaccgtgac tttaccgggc gaattttacg gcgattcaga agcacaagtg aaggtaaaac
    15001 cggttataca gtgcgtatac cattgcacaa acaatctcaa caatttacct ggtttcgatt
    15061 cgagtctcga ttatgtggta tccataaatc cgtacaatta ctttataaac aacatctatc
    15121 tcgcaaacat agtcatatgt tcttctgtca cagaagcgaa ttccgcagat agctatctta
    15181 ataccgatta tgtggaaaca atgacaatcg aatcaatcag tcaggaacca aattatgcgg
    15241 cagatagccc gcttaataag gaaatggtgt ttaaaatagc aatgttcgtc ggttcaaaat
    15301 tgccattggt aggatcatac ttcagtctga taagcagaag taaaaccgga atggaaatac
    15361 taaacgggtt gttattctgc gataaaataa tccaaggaga catagtcgat cagacagata
    15421 agtcaataaa aattgatatt tctgaatcat atactacaca tataaaatat tttgaaggct
    15481 ggttacaatc ggtagcctta ggggcatatg agggtacaga gaatattgaa gtgggagata
    15541 atgccgatta taattatctc tacaattcat tgaacggttg cttcatgaaa acaaccgtcg
    15601 aatatacatg cgactttcct gtggacgctt cgggatataa gatcgaattg agcagcgaaa
    15661 tgaatttcag cacctgcata tcgcttgaca ttgtaacaca agacgaaagc acattcgaac
    15721 ttacaaaagt agcggaagtg tgcggcgaag cggattacgg taaataccag cgctatggtg
    15781 aattaatgaa tatgtatgaa cccacggctg ttgtcattaa tgatccaggc accgccgatt
    15841 attttaaaat cgcagcggat tataacggaa tttatcaaat aaagaagacg ggatgcagca
    15901 ccaatacaag aattgaaatt tatgataacg ttggaaatct tgttgtcagt tccacagcag
    15961 ttgactttac gttcagagta aattcggaca tagagtatta tgtgaaaacc aaatacttaa
    16021 gcgatgacta taccggaaca tataatatac ttgtcatgcc tcagtacatg gggttgttgt
    16081 cgacagatta ctataagtat atatatttta cagagccaaa agccgtaaca tattatttct
    16141 gtccgacgaa atctgcggat tattacatat atacgggcgg aactcaggac actttaatta
    16201 aactgtacga caaatacggc aatttcatta ccgccaatga cgacaacata aatgatgata
    16261 acgcaaagat actcgtgcat ctggttgccg gagagacata ttatgttcag ctggtaaaca
    16321 aaagcacaac ttggggatat gcgaaagtct atgtgctgga atgcgtatac atagatccgg
    16381 atcctttcta tattcccaaa gacggaatag aattaatgta aaaaacaata aacatacaag
    16441 cggattatca gtgctggtta ttacatgcat ccgagtaaaa aagaaaaccg gaagtttctt
    16501 acgaaacccg gttttttata ttctacgttt cgtagaaatc aaaatatctt gtcttttggt
    16561 tatttggatg gtttaattgt ctcaggagct gataatatga cgatcggtga aaaaatagcc
    16621 gcaagacgca aagaaatggg atacagtcag gctgaactcg cagccaggct gtatgtaagc
    16681 gacaaaacga tttccaaatg ggagaccgga cgcggaaagc cggaaatcga taccttgaaa
    16741 aaaatctcga ctgttttggg aatgaccgtc gacgaactgt tggatgacga tatggaaaac
    16801 caggataccg gcaacgcaaa aaaaagcaaa cggtccttca tggaaatcat atcggagaag
    16861 attccaccgc tttcctacaa taaaaacgac attgccgtag tgtgggtctt gggtatcggc
    16921 cttggcattt tgctagatat cattctcacc tggtggtttt taaggatcgt caggatatat
    16981 gggttggatt acccggtagg ccttttagtg ctccaggtga ttaccacgac tatgctgata
    17041 gttgccatag ccaaagcgaa aaccgatttg cgttttctgc tcgcatacat ggtatttata
    17101 gtgatccttt tgatttacac gcttttttcc gcagatcgag actttctgga agtcctgaca
    17161 attctggcgg agattgcggt gccggtattg tttatccttt acatattcgg agtcgtttcc
    17221 gaaagaagaa taccgttata tatccaactt gtgacgatca tattggcggg aatcactttc
    17281 ctgacaagaa tattcgactt caatttgttc gccaattctt cgtttaccta tgtatacaat
    17341 gtcatcaaca gtttcaattt catcattacc agcatagctt ttttcttcct gttgaaaggc
    17401 gtcttgggtc cggtgtcgga tatgccaatt caaaaagaac cctccgaagc cgaataaaca
    17461 aacaaataat gaccgaaggc aaggaatttt ccttgccttt ttgaatgcca aaaacaaagt
    17521 gcgttattta tgatataatc ataatgaaca acatccaggc ggtgataatt tgcaaaaaaa
    17581 gaatatcacg gtaataggcg gcggagcatc cggcgtcatg gcggcgcttg cggcagcgga
    17641 agacggagcg tcagtcaccc tgctcgaaag aaattcccgg atcgggaaga aattgcttgc
    17701 gaccgggaac ggtcgctgca attacacaaa cgagaatctg acggcggatg attacaacca
    17761 tcctgatttc gtcaagcacg ggcttgcgca gttttcttcc accgatacgc tggttttttt
    17821 caagaatctg ggaatcgatc ccaaatacga agaagaaggg aaagtgttcc cccgctccga
    17881 acaggcttcc agtttcctgg acatatttct ttacgaactc gatcgtcgtg gcgtcaatat
    17941 agtaaccgac gcctatgtcc aaaacatagt cgcaaagaaa aacggatttc tgattttctt
    18001 ggcggacgga accagttaca aagccgacaa ggtgatcata tcgaccggcg gaaaggcgat
    18061 gcccaaaagc ggaagcgaag ggtcgggata tgtcatcgcc caatcgctcg ggcatacgat
    18121 aacggatgtt ttccccgccc tcaccaaact gaaaatcgac agcccttatc tgaaacaact
    18181 ggacggagtg aaatttcccg gagcggtaga attgtttgcg gacaatgttt ccttgcaaaa
    18241 ggaaaaggac gatattcttt tcaccaaata cgggatcagc ggtcccgcga tcatgcaact
    18301 gagccggaaa gccaacgaag ctttggcgaa aaaacaaaaa acggaaatca aagtatccat
    18361 aatagacacg ttgtcaaaaa gggatattgc caaaagaatg gattcgtcca tggacaaacc
    18421 ggtcgattac agtctaattg ggcttgtgaa caaaagactg atcgccgcct tgttaaagga
    18481 agcgggagtg gaaaaacaaa atgcgcttgt gtcaagcctc cgggacgaca caagaaacaa
    18541 aatcatcgat ttgttgtttg actggcgttt ccctgtcaaa ggatcgatgg ggtttgacga
    18601 cgcccaggtt acggccggtg gcgtcagttg cgaggaaatc gatcccaata ccatggaatc
    18661 caaattggta aaaggactct atttcaccgg cgaaatcatg gacatcgacg gcaagtgcgg
    18721 cggatacaac cttcaatggg cctggacaag cggctatctg gccggggaaa atgcggccaa
    18781 ggatatggaa tatgacgatt aggataaccg aaatcaagct tcgtccggac gaggcgatag
    18841 ataaagagtc ggaacttttt catctgaagg acaaagtctc gagaaaattt cgaattgtgg
    18901 aaagcgatat gttttccttc aacataacga gaaaagcgat cgacgccagg aagaaagaag
    18961 acatttttct agtatattcg gtcgacatgg aactgaaaaa cggaaaaagc ttgctcgcaa
    19021 agggttttcc gaatgtcaag gcatatgaga aaccggatat caactatccg ggaaaaccga
    19081 agataaatcc gaaatatcgt ccggtgatcg tcggtttcgg acctgccggc atttttgcgg
    19141 cgcttacctt ggccaagcag ggactttccc ctcttgtcct ggaacaaggc ccggatgtcg
    19201 acaaaaggac cgaggcggtc agttcttttg gcaaaacagg cgtgttttct cccgaaggca
    19261 ctgttctttt cggagaaggc ggagccggaa cgttttccga cggcaaactg acgaccttga
    19321 tcaacgatcc cttatgcgac atcgttttgg atacctttat cgaacacgga gccgatcccg
    19381 agatcaaata ccaggccaaa ccgcatatcg gcaccgatgt cctgaaaggc atcatcaaga
    19441 gtatcagaaa agagatcatg gatcttggcg gtgaagtcag attcaatgcc aaggtgaccg
    19501 acttcatcat caaaggaaac aaactggaag gcattgtcgt aaacgatgat gaaaacatcg
    19561 aagcggatat gtgcattctg gcgatcggcc acagcgccag ggacacctac gaactcttgc
    19621 tggataaagg gttgaaaatg gaacagaagc ctttttcggt cggagtaagg atcgaacatc
    19681 tccaaaatgc gatcgacgaa gcgcagtacg gcaaattcgc cgggcacaag gctttgggcc
    19741 cggccgatta caagctggcg taccatgccc ccagcggacg gacagcttat actttttgca
    19801 tgtgcccggg cggtttcgtg gtgaattcct catctgaaga aggaggaata gcgacaaacg
    19861 gcatgagcta tagcaagcgt agtggtgtaa atgccaattc cgctttgctt gtgaatgttg
    19921 gaccgggaga ctacggttcg gatcatccgc ttgccggaat cgagtttcaa agaggatttg
    19981 aaagaaaagc aaataatctt gccggaaaca aatactatgc gccaatacaa tcgttaggca
    20041 attttcttga tgacaggatc acaaatgaga tcggatttgt aaagccaaca tatctgcctg
    20101 ataccgtgtt tgcacaaatg gcagacatct atccagatta tgttatcaag acgcttaaag
    20161 aggcattgcc cgtatttgac aagaaaataa aaggatttgc gtcaaacgat gcaattctga
    20221 ctgctccgga aacaagatcg tcatcacctg taagaacaac aagaagcagt gaatacgaga
    20281 cgaatatcga aggaatatat tcaatcggag aaggatccgg ttattccgga ggaatcatgt
    20341 cttcggcaat agatggaatc agatgccaat caaaatgata cattaacgaa aaacggaggt
    20401 ttctcatgcg cttgacaact ttcattgata aaggcaaatt ttataaggcc aatctgcacg
    20461 gccattccac ccggagcgac ggatcgctga cccaggaagc cgccgtcctg gcttataaga
    20521 acaaaggcta tgattttctg gctttgacgg aacataatgt cattacaaaa cttgacaaat
    20581 acaataccga cagtttcatc ctgataccgg ggatggaact ccatacctat aacaaagaca
    20641 tcacgaccat ccatcatatg gtcggtcttt ccgatatgga caacgatttc atcaaggacg
    20701 gaaccgttct tgaaaacatc gtttataccg aaaacaagac ttcgcttctg gctttggaga
    20761 agagattgca cgatctcgga ttcctgacga tctactgcca tccgacctgg agccatgtga
    20821 acacttccca gttcgatttt ctcgaacata cgaatatgat ggaagtctgc aactacggca
    20881 caatctggga aatgtcccat aacgaggacc cgacctattg ggacgattgg ttgaaacagg
    20941 gaaaacagat atatggcgtc gccaatgacg acgagcatgt gatagagcag tatttcggcg
    21001 gctggaacat ggtgaaatgc gaggagttct cgatcaaatc attgttcgaa agccttaaaa
    21061 aaggcagttt ctacagcact atgggcccaa tgatcgaaga tttctatgtc gaagacggca
    21121 tcgcccatat cgagtgctca aatgtgaagg aaatcacctt tgtcgtccat ggttggtggg
    21181 gccatacttt cagggataag gaagggaaac ttaacaaggc tgacttctct cttccggatc
    21241 gtgcctacat cgtcagggcg gaaatcaccg accttgatgg aaatgtcgcc tggaccaatc
    21301 cgatcatgtt gccaagaaat taaaaaaagc ccggtggtca gtaaagtgaa ccccatttag
    21361 tggacacgaa ataaaaaggc acaaacgaaa gatgttagta agcaaatgaa tttgtttatt
    21421 agcatctttt ttgttttagt caagtattta tccagaaggc attcaatcct gtctgaaatg
    21481 gtaatattat ggaaagcgaa agatagtgaa atatccacaa aggagggatt gcgaatggca
    21541 agcaaaggcc aaaaattcaa gaaagtgccg ctggagacga agataaaagt tgtaaaagaa
    21601 gggcttgatg aaggaaaaag cggttattat ttgggaaaga aatacggtgt aagcaggaag
    21661 acggtcgcta catggatacg aatctataaa cgggatggcg gactggatat tcgcaagaaa
    21721 ggaaggccaa aagctgatgg ccagattgat tacaaggaga gatatgaaat cttaaaaaaa
    21781 tatcaagatt acttagggga ggtcgatcgc gagaaaaaat agcattcatc acaagatacc
    21841 gccgcaagta ccggatcaag acgatgtgcg aggtgttgga gatcagcaca tcgaac
//
LOCUS       DAOPJL010000022        19916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_2871_length_19916_cov_6.320125, whole genome shotgun sequence.
ACCESSION   DAOPJL010000022 DAOPJL010000000
VERSION     DAOPJL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2871_length_19916_cov_6.320125"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLP02_04000"
     CDS             <1..674
                     /locus_tag="PLP02_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPF42640.1"
                     /translation="HVYQTSWGVTTRLIGAIIMVHGDDSGLVLPPRLAPVQAVIVPIQ
                     QTREGILDKCNEICDKLLKAGVRATVDSQDKSPGWKFAESEMRGIPIRIEIGPRDLEN
                     GDCVIVRRLDNQKIVLKWDEAIAMIPDMLEAIHAEMYAKALANVKSNTHDAYTYAKFK
                     EIMAKEGGYVRMMWCGDRACEDKIKEETQATARCIPFTQEVITETCPVCGKKATKIVY
                     FAKAY"
     gene            702..1109
                     /locus_tag="PLP02_04005"
     CDS             702..1109
                     /locus_tag="PLP02_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HPF42641.1"
                     /translation="MRYKTIILTDKLTFASNHLAKGQYKVNPKITRNVGKINPQTYFT
                     QINVEIKDSEPAHFPFNLEVSLRAIFEFQDFPPENEIYDFLKIPAVNILYPYVRSAVT
                     SLTAMSMNPPIVLPIVDASVLFTDAAQDSYIIS"
     gene            complement(1136..1663)
                     /locus_tag="PLP02_04010"
     CDS             complement(1136..1663)
                     /locus_tag="PLP02_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPF42642.1"
                     /translation="MKKIISPICDKDLLSLEAGDEILLTGTIYTGRDAAHKLMCQAVE
                     KKEKLPFDIVGQTIYYVGPTPAKPGMPIGSCGPTSSYRMDRYSPILMKHGLKVMIGKG
                     ERSEEFRKLMELHQGLYLIAIGGIGAKLASTVKKAELVAYRELESEAIYRLEVEDFPC
                     IVAYDMHGGNVFKQK"
     gene            complement(1660..2514)
                     /locus_tag="PLP02_04015"
     CDS             complement(1660..2514)
                     /locus_tag="PLP02_04015"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008800786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPF42643.1"
                     /translation="MYAINRMILISKIRDMIIEANTRLSEEVTCQIGNALKTESEPLA
                     KNVLENILENQKIARSLDLPLCQDTGVAVFFVRVGRELYFDYSLTEALNEATARAYRE
                     GYLRKSIVKHPLDRINTSDNTPAIIHLIETEGNQLDIMFAPKGGGSENMSRLCMLTPA
                     EGRQGIIDFVLETIKLAGGKACPPLIVGVGIGGNFEMCALIAKEAIFRPLSDEASNPV
                     DRELENELKEKINALDIGPMALGGKTTCLAVKVNSHPCHIASLPVAVNLQCHSARKSH
                     LVIEGEKL"
     gene            2688..3542
                     /locus_tag="PLP02_04020"
     CDS             2688..3542
                     /locus_tag="PLP02_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPF42644.1"
                     /translation="MPKIKIITDSTCDLTPDLLEKNDIAVLPLHVSFKDITYEDNVDI
                     NTDRLYELVEEHGYLPTTSATSPGVFYEAFKKYLSEGYDIIYTGIGNELSATMKAAYM
                     AKQMLETGADHVYIVDSMNLSSGIGLLLLKAVKFRDEGMDAKTIADRLEAIAPKVSTQ
                     FVIDTMQYLYKGGRLSALSAFMGTVLKIKPIIRVRNGVLQVGKKPRGSIINGVKILVD
                     EVLALGDQIDDDFLMITHSHAEESAEYIKKQLIGKIPVKNIYETFAGCVISSHCGPGT
                     IGILYIVK"
     gene            3642..3941
                     /locus_tag="PLP02_04025"
     CDS             3642..3941
                     /locus_tag="PLP02_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42645.1"
                     /translation="MKRNILHFNPILFAKIPGGLTLSRSSSINEGEKQASELRMIPEC
                     FEKKHSAETYDNHKTPSCELYRFDQAFFCARKNEDIRLYLENDHYFVTPVQKAMS"
     gene            3938..5233
                     /gene="rimO"
                     /locus_tag="PLP02_04030"
     CDS             3938..5233
                     /gene="rimO"
                     /locus_tag="PLP02_04030"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPF42646.1"
                     /translation="MKAAFISLGCAKNLVDSEFILGFLEKSGIAIVNDPEKADVIFVN
                     TCAFIEPAREETRETIAEMHAYGKKIVMLGCYAKRYRDRIQKEMPYVNKVVSLDEYPD
                     LAQIMKDFFKDDSLSFAKLDYHNRMLTGNPYSSYVKISEGCDNRCAYCAIPMIRGQFR
                     SRPYKEIIDECRLLIKNGAKEITLIGQDTTKYGTDLNDNVTLASLMKEIAAIENVFLV
                     RVLYLYPDEVTDELIEAMVSNEKIAPYFDLPIQHISTPLLKLMNRRGDENDIRALLKK
                     IKDKKSDAIIRTTLIVGFPGETDADYSKLADFVFESGFDRLGAFMYSKEEGTKAFSME
                     NMVLPEVMQNRYDNIMRIQRRVSRKQNQKQVGLIHKTLVENYDPETGFYYGRSYAFAP
                     DDVDGYIVFKSKRELKMGEVVDVLIKECIGYDLIGDEIG"
     gene            5247..6182
                     /locus_tag="PLP02_04035"
     CDS             5247..6182
                     /locus_tag="PLP02_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPF42647.1"
                     /translation="MEFILLLLKGFVIGIAFIIPGVSGGTVAFYLGIYDRIIHAIGHL
                     FSEFKKSMSILIPVALGIMISVVMLAKLMAWLLNVNSFMTMLFFIGLLLGGIPGLIGQ
                     MDDKPFKKTRTLIPMIVGFVLVMVLLVAERKASGDAVASIDISFANFWLLFLLGAISA
                     ITMIVPGISGSALLMVLGYYTAIVTGVFKNLFDYASFGYNIYVILSYGFGAVVGVLLF
                     SRPLDYLLTNHRRETLAAIIGFVAASVIMIFLEIRDPATNAEFVEQTPVYLDFWNFAR
                     ANIGSIVGGILLGVGGFLAAYFMIRPKKADPEDVG"
     gene            6172..6963
                     /locus_tag="PLP02_04040"
     CDS             6172..6963
                     /locus_tag="PLP02_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPF42648.1"
                     /translation="MSVKRIEAKSFMNKSELDGDLTCNPYYGCSHHCIYCYAKYIMQG
                     PVTSKNWDEDILVKEFSNYDIPKGTGPARLLLSTATDPYQPLEMKEKVTRKILEAIVD
                     SDLHVSILTKSKHVIRDIDLFKRMKDVSVSFSIALDDEMSAIIEPGASRPSERIEALK
                     ILHGENIRTGVLMAPILPYLSDVFSIIDKIRGYADSVMLDTLNLKNPEHIRHVMAFIA
                     TRFPEYYEGYRKIFIKKDKTYYQKLKEEILACESETPFGLKHIYK"
     gene            6975..8153
                     /locus_tag="PLP02_04045"
     CDS             6975..8153
                     /locus_tag="PLP02_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPF42649.1"
                     /translation="MFTLDDIRPYENQIVAWRRKIHSWPETGFDTIKTHDYIRTELEN
                     EEIEVHTHVGTNSIVGIIRNGTAPIIGLRADIDALPLQEETRDRGFKSQRDGFMHACG
                     HDAHAAMLLGAAKLLCNNKTKWSGTVKLIFQEAEEGPDPGGAYGIVESGLLDDVEDFY
                     ALHVSPDNPAGVFVTKSGNIFASVAIFRITAKGKGCHAAYPEMGKSPIIPLARIVEKI
                     QDIVPNRPNKNEKLVVSVTQVHSGTTHNIIPETAMTEGTIRAYSKEAREYVRKSMEKI
                     IEDVSSITGVEFDFSFRDVYEETANTESSFIRFRNMVVAAFGKDHFMEMPEALMGGED
                     FFRYIKKQKGCISWLGTRKDESTAYSLHSPFFDLDEDVLKYGAVQLANLATGIERKDK
                     "
     gene            8150..9328
                     /locus_tag="PLP02_04050"
     CDS             8150..9328
                     /locus_tag="PLP02_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPF42650.1"
                     /translation="MMYLIKNANLIGMKDQNYVKTDILTEGQKIKAIGKLNPDKYPGC
                     VVIDAKGNYVTPGIVDAHCHVGIYEEAIGFEGLDVNEMTNPVLPELRAIDGIKPQDVA
                     FKDALEAGITTVSTGPGSANVIGGTFCILKTYGKTLDDMLVKEESAMKMALGENPKRV
                     YKSKNQAPETRMGSAALIREALIKAREYLAKKQKYEEDIRAGKEVSKPDFNMKWESLS
                     RVFKGLPVKIHAHQQDDIATAMRIIGEFGLDGTIEHATEGYLIPEFLHEHGQRVIIGP
                     TLGSKSKYELRNKSFKAAKVLFDNQIKFAIMTDHPVINLANALTQVGLFVREGLPELE
                     GFRAVTINAAELIGISDRVGSIEIGKDADIVIWNNHPLHYLAKPEYVFVNGEIVVENK
                     "
     gene            complement(9341..9838)
                     /locus_tag="PLP02_04055"
     CDS             complement(9341..9838)
                     /locus_tag="PLP02_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPF42651.1"
                     /translation="MRMKNVVEAVIEIPMGTQNKYEVDKQKNRIKLNRVLYSKMTYPA
                     EYGFIEETLAEDGDPLDILVLATTQTFPGCIVDARIVGFLDLYDSGDHDEKIIAVVDA
                     DPRFEDVNDINDIKDLQLKEIKLFFRNYKELQPGHHVEVLDYYPLANALELIESCRQR
                     YRESK"
     gene            9939..10334
                     /locus_tag="PLP02_04060"
     CDS             9939..10334
                     /locus_tag="PLP02_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42652.1"
                     /translation="MNLHELFRFQAGWLLSNWWWLLPSFLAVLMIVLYLANVLKKASE
                     HKPEIDMAKLDRIIAKLGGLENILETSGDGNRLKFTVKELKKCDLVAIKEDGVQGIFV
                     AGKQVKFQLPEMSEALLNRIEEGKKEATK"
     gene            10331..10597
                     /locus_tag="PLP02_04065"
     CDS             10331..10597
                     /locus_tag="PLP02_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPF42653.1"
                     /translation="MIENKYKITYPYGLHARTGTMLVAKANGFKSEMVIEFDDRKVNL
                     KSIMGVLSLAVPANRSFRIMITGSDEEEASQAIARAIADINEMK"
     gene            complement(10633..11631)
                     /gene="trpS"
                     /locus_tag="PLP02_04070"
     CDS             complement(10633..11631)
                     /gene="trpS"
                     /locus_tag="PLP02_04070"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPF42654.1"
                     /translation="MKRMLSGIKPTGILTLGNYIGAIKEFVRIQNELEDTEFYLFIAN
                     LHAITTEQDPAELRKRSKMIAAAYLAAGMDPKKTVLFNQSEIKEHAELGYLLQCFTYM
                     GELERMTQFKDKAAKQETGITSALFTYPVLMAADILLYDADYVPVGEDQKQHVEITRN
                     IAIRFNNRFGDFFRVPEPLIPKVGARIMDLQNPLKKMSKSDESDKGYIALLDDEATIA
                     RKIRSAVTDSIGIVRYDPENQPGLANLIDIYASLSGLSSDEIVLKYTGCNYKIFKEDL
                     VGVVTSVLKPFQERFYTIINSSELDEMLDNGRKIAGEYAFKKVEKCKYKLGLSRKR"
     misc_binding    complement(11668..11860)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11872..13644)
                     /gene="pepF"
                     /locus_tag="PLP02_04075"
     CDS             complement(11872..13644)
                     /gene="pepF"
                     /locus_tag="PLP02_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPF42655.1"
                     /translation="MNVKWDLTYLFPNVETYDKAYRKTLDLIDKLATFKGKLGDEADF
                     KVYFLLQKDLQKTGWRAYQYAALLSDLDKKNTENASRLQLMQIAFAKLQQAVSFEEPE
                     LISIGREKVLSFVDNNPELKEFRFGFEKLFRRQEHILDDKSESLLAGFGKLADAGKDL
                     YGSLSVADIEHQDVMLDSGDIVQITNSNYRAYIQKSQSPSERKDIFDSVFSYYDVHKN
                     TYASIYRLVLDYDIAMMKARKYPNSLESYLFKDAIPASVYKSLCNVARKNTGVIKKYL
                     KIRKDYLGLEEYHTYDRFIPLARSDKEYGYDEARNLFFSSLEKFPADFKNKAMDVLKE
                     GFVDVYEQPGKRTGAYSSSMPDTHPYILLNYSGTLEDVFTVAHEAGHSIHSLYSAETQ
                     PSALQDYTIFVAEVASTFNEHNLLDYFIRESKATKNEKIMLLQRAIDDIMSTFYRQTL
                     FAIYEAEAHEKAENGEAITADVLSDIMIDLYKEFYGLDIVKEEVKQYVWAYIPHLFYT
                     PFYVYQYATSFAASLKLYENVKNGVPGAFENYIGLLKSGGSDYPVNQLQTAGVDLTAD
                     DAFLAVVRRMEELVGELEVAIAEK"
     gene            13759..14199
                     /gene="spx"
                     /locus_tag="PLP02_04080"
     CDS             13759..14199
                     /gene="spx"
                     /locus_tag="PLP02_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HPF42656.1"
                     /translation="MITLYTSSSCSSCRKAKKWLEENRIPFKEKNIVGMKLTRDDIIN
                     MLKYSDNGFEDIISTRSKVFKDNNLDTEDMRFGELADFIIDNPTILKRPIIINDKIMQ
                     TGYNEDEIRAFIPRKFRENIVCSECDEDCEYKQCIKEAFEKNKK"
     gene            14345..15352
                     /gene="gap"
                     /locus_tag="PLP02_04085"
     CDS             14345..15352
                     /gene="gap"
                     /locus_tag="PLP02_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPF42657.1"
                     /translation="MSVKVAINGFGRIGRLAFREMFGKPDFEIVAINDLTDAVQLAYL
                     LRYDSSQGRYEKNISTEGNMLVVEGVKVPVFAEKDPSNLPWKDYNVDVVLECTGHFTS
                     RDKASLHLQAGAKKVIISAPAKDKDIPTVVFGVNETSLTGEETIISAASCTTNCLAPI
                     AKVLDDNFKIVKGFMTTVHAYTNDQATLDIPHPKGIYARRGRAAAANIVPTTTGAAKA
                     VGLVLPQLKGKLDGIALRVPVITGSVVDLVVELGKPVTKEEVNLAVKNAASDTIGYTE
                     DPIVSSDVIGITQGTMFDALSTTILEVDGKQMVKVITWYDNEYSFTAQMIRTLKYLAE
                     L"
     gene            15413..16030
                     /locus_tag="PLP02_04090"
     CDS             15413..16030
                     /locus_tag="PLP02_04090"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPF42658.1"
                     /translation="MEWLYALLDKFELFISSNVWFAPLSAFILPFIEALIPSLPLSVL
                     VAFNLNIMQQVYGEIVGTGLTVFLSVLGSFAGMFLIFGLIRLTLEPYFARKVQNHKYG
                     KMFLEIAEGSKSVPILLLMSNPFLPSSIINYALSLTKIKTGRYIWLTIVSRFFVMMFL
                     ILLGSLFDVQNHLINIVWFTLAYSLLFVLWYIWFRIRRRKKENIK"
     gene            16115..17380
                     /locus_tag="PLP02_04095"
     CDS             16115..17380
                     /locus_tag="PLP02_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPF42659.1"
                     /translation="MDRAFYKKHREDFLARMDSQSFMVLFSGKAPHKSADQKYQYTPN
                     RNFFYMTGIGRENMILLLIKGKSVGHEYLFIEEPSDYMTKWYGPRMSKEEAANISGID
                     SGSIRYKGEFSGFLAQSVLTDSRKNLAGSIKIAYFDMYRSEPLAKPVNHPYFETIIDN
                     YPELEIKNACEIIDLLRMVKDESEIAEIEKAIAYTKKGLDAIFKTVRPGVNERELEAV
                     FAYEIRRQGSEGISFDTIVASGMNATSLHYEDNNCQINGGDLVLTDLGALSGPYAADI
                     TRTFPASREFTTRQKKIYELVLSVNKACIAKVHPGLVMADLFSFARDELARGLIRLGV
                     MRDMSEIDKYYYHSVSHYLGLDVHDVGTYQLPLVPGVVLTIEPGVYIEEEGIGIRIED
                     DVLVTAKGSRNLSESIPKEIAEIERLLAK"
     gene            17399..19639
                     /locus_tag="PLP02_04100"
     CDS             17399..19639
                     /locus_tag="PLP02_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPF42660.1"
                     /translation="MGFLDGNIRQYLFYNEENSYSVIKVEIIDTDEPELSHFEPTITI
                     CGFFPRLELYQTYRFHGVVSEHQKYGIQYSADRFERIREQTASGIIDYLSSDLFKGIG
                     PKTAEAIVGILGIEALDKIADDPSVLNEIPRMNAEKKAILSETIRRNRAMESALVWLY
                     GFSISPKMAMKIYQRFGFATIETVRNNPYSLIDEIEGIGFRRADEIALKIGFAYDDPL
                     RIEAIIVFLLTEYMNKYGDTYVESDELMNYTINYLNQGDQFQIKTETVDKEIEKLENS
                     GKLVKDGNRVSLMAIYYSEKGIADSLKEYFVSKNEAYDEEMIAGYLEAFEKSRHIQYT
                     DKQKKAIMKALTESFTIITGGPGTGKTTVIDAICHVYMMMRQSNSNFTSAVKLAAPTG
                     KAAKRLQDATDLEATTIHRLLGYDYEGHFTKDRHSPLEAGLLVIDEASMMDVILANHL
                     FQSLSDGTKVVIVGDDNQLPSVGPGQVLTDLMASDQFTTVGLDMIHRQASGSSIVKLA
                     YSVLEGTIEESVFHDKDDLFFYGVPDEKIADFVVGLIRRYLREGYNLQEDIQILAPVY
                     KGNNGIDRLNKIVQETFNKHNEGHKLTYKDKTFRFNDKVMQLVNQPEDGIMNGDIGYV
                     SGIIEDKELLVDFGGNTVKYDIRDFDNLTLAYCISIHKSQGSEFNVVLMPMSRSYRIM
                     LKKKLIYTGITRAKQLLSIIGEKDALRIGIYGNETPRKTRLRDFLALAAKSGNDDGAL
                     TIEDFL"
     misc_binding    19688..19877
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         5528 a         4399 c         4660 g         5329 t
ORIGIN      
        1 aacatgtcta tcagacatca tggggtgtca caaccaggct gatcggcgcc attatcatgg
       61 tccacggcga cgattccggt ttggttctgc ctccacgtct ggccccggtc caggctgtca
      121 tcgtgccgat ccaacagaca agggaaggca tccttgacaa atgcaacgag atttgcgata
      181 aattattgaa agccggcgtt cgcgccacag ttgacagtca agataaatct cctggctgga
      241 aattcgccga atcggagatg cgcggcatcc ccataagaat cgaaatcggc ccccgcgatc
      301 tcgaaaatgg cgactgtgtc atcgtaaggc ggcttgacaa tcagaaaatc gttttgaaat
      361 gggacgaagc aatcgccatg atccccgaca tgctggaagc gattcatgca gaaatgtacg
      421 ccaaggcctt agccaatgtc aagtccaata cgcatgatgc ttatacatac gcaaaattca
      481 aggaaataat ggcgaaagaa ggcggctatg tccgcatgat gtggtgcgga gaccgtgctt
      541 gcgaagacaa gatcaaggaa gaaacccagg caacggcacg atgcatacct ttcacccagg
      601 aagtaattac ggaaacatgt ccggtatgcg gaaagaaagc gacgaaaatc gtttattttg
      661 ccaaagcata ttgatgtgtg ttttgaagga ggtatgtaaa aatgcgttac aagacaatca
      721 ttctgacaga caaactgaca ttcgccagca atcatctggc caagggacaa tacaaggtca
      781 atccgaagat tacccgcaat gtcggaaaaa tcaatcctca aacctatttc acccagatca
      841 atgtggaaat aaaggattcc gaaccggcac atttcccctt taatctggaa gttagtttgc
      901 gggctatctt cgagtttcag gattttccgc ccgaaaacga aatctatgat tttttaaaaa
      961 ttccggcggt caatattctt tatccgtatg tccgttcggc cgtcaccagc ctgacggcga
     1021 tgtccatgaa tccgccgatt gttttaccga tcgtcgacgc atccgtgctt tttacggatg
     1081 ctgcccagga tagttacatt atcagttaaa aaaaagggac ttcataagaa gtcctctatt
     1141 tttgtttgaa tacattgccg ccatgcatgt cgtaagcgac gatgcatggg aaatcctcaa
     1201 cttccagtct atagatcgct tcgctttcca gttccctgta ggctaccagt tcggcctttt
     1261 tcaccgtgct tgcgagtttg gcgccgatgc caccgatagc gatcaggtaa agaccctgat
     1321 gcagttccat gagctttctg aattcttccg accgctcacc ttttccgatc atcactttca
     1381 atccgtgttt catgagaatc ggtgaatatc tgtccatcct gtagctcgat gtggggccac
     1441 atgaaccaat aggcattccc ggctttgcgg gcgtcgggcc gacataatat atggtctgtc
     1501 ctacgatatc gaacggaagt ttctcttttt tttccacagc ctgacacatg agtttgtgag
     1561 cggcgtcccg tccggtgtag atggtgccgg taagcaatat ttcatctcct gcttcaagcg
     1621 ataaaagatc cttgtcgcat atgggcgaga ttatcttttt catagtttct caccttcaat
     1681 cacaagatgg gattttctgg ccgaatggca ttggagattg acggccaccg gcaaagacgc
     1741 gatgtgacaa ggatgagaat taactttaac ggcaagacag gttgttttac cgcccagagc
     1801 catcggaccg atgtcgagag cattgatttt ttcttttaat tcgttttcaa gttccctatc
     1861 gacgggattt gacgcttcat cgcttaatgg ccggaatatt gcttccttgg caatcaaggc
     1921 acacatttcg aaattgccgc caatgcctac gccgacaatt aacggaggac aagctttgcc
     1981 tccggcaagt ttgattgttt ccaatacaaa atcaatgatt ccctggcgtc cttcggccgg
     2041 agtcagcatg cataaccggc tcatattttc gctgccgccg cctttggggg cgaacatgat
     2101 gtccaactga ttgccttcgg tttcgattaa atggatgatg gccggtgtgt tatcggaagt
     2161 gttgatcctg tctaaaggat gcttgacaat ggatttccgg agataacctt ccctgtaagc
     2221 tctggctgtt gcttcgttca aggcctctgt cagggaataa tcgaaataaa gttcgcgtcc
     2281 tacacggaca aaaaagaccg ctactccggt atcttgacac aaaggcagat ccagcgatct
     2341 agcgattttt tggttttcga gaatgttttc gaggacgttt ttcgccaacg gttcgctttc
     2401 cgtttttaat gcgttaccga tctggcaagt cacttcttcg gacagcctgg tattggcttc
     2461 gatgatcatg tccctgattt tggatattag aatcattcgg tttatcgcat acatcatacc
     2521 acttcctaat ttaattatat catttgtgtg acgataggac ttaacttttt tccgtattca
     2581 atcttgaaag tgttgcaaaa gtgttttgcc agcggtcatc attgaaaacc ccgctttact
     2641 tttttgttcg tattttgtat aatttataca tgatcggtgg tggagaaatg ccaaaaatca
     2701 aaattattac cgacagcaca tgcgatctga cgcctgatct gttggaaaag aacgatattg
     2761 ccgtattacc tttgcatgta agtttcaagg atataactta tgaagacaat gttgacatca
     2821 ataccgacag actctacgaa ctagtagaag aacacggata tctgcctaca acgtcagcca
     2881 cttctcccgg agtcttttac gaagctttta aaaaatatct gagtgaaggg tacgacataa
     2941 tttataccgg tattggcaac gaattgtccg caacgatgaa ggctgcctat atggccaaac
     3001 aaatgttgga aaccggggcc gaccacgtat atatcgttga ttcgatgaat ctttcttccg
     3061 gaatcggatt gttgctactg aaagcggtca aattccggga cgagggcatg gatgcgaaaa
     3121 cgatcgccga cagactggaa gcgatcgctc ccaaagtaag cacccagttt gtcattgata
     3181 cgatgcaata cctttacaaa ggcggccgct taagcgcgct ttccgctttc atgggaactg
     3241 ttctgaaaat caaaccgatc atccgggttc gaaacggagt ccttcaagtc ggaaagaaac
     3301 cgcgtggaag catcataaac ggcgtcaaga ttcttgtgga cgaagtgttg gctttaggag
     3361 accagatcga cgacgatttc ctgatgatta cccattcgca tgcggaagaa agcgccgaat
     3421 atatcaagaa acaattgatt ggcaagattc cggtcaaaaa catttatgag acgtttgctg
     3481 gttgtgtgat atccagccat tgcggtccgg gaacgatcgg aatactttat atcgtaaaat
     3541 aaggtttgcg taaatcgcaa gccttttttc ttttcatgcg actttatttt tttccttccg
     3601 cagcgtataa tatgaatatc atcgcaaaag aaggatctac catgaaaaga aacatattac
     3661 acttcaatcc gatattgttt gccaagatcc cgggaggatt aactttatcc cgttcaagca
     3721 gcataaacga aggcgaaaaa caagctagcg aattgcggat gattcccgag tgctttgaga
     3781 aaaagcattc ggccgaaact tatgataatc ataaaacgcc gtcatgcgag ctttatcgat
     3841 tcgatcaggc gtttttttgc gcccggaaaa acgaagacat tcgtttgtat cttgaaaatg
     3901 accattattt tgtaaccccg gttcagaagg ctatgtcatg aaagccgcct tcatatcgct
     3961 tgggtgcgcc aagaatcttg tggattccga gtttattctc ggttttctgg aaaaatctgg
     4021 cattgcgatc gtcaacgatc cggaaaaagc cgatgttatt ttcgtgaata cttgcgcttt
     4081 catcgaacct gcccgtgagg aaacccggga aacaatcgct gaaatgcatg cctatggcaa
     4141 gaaaatcgtc atgctcgggt gttacgcaaa aagatatcgg gaccgcatac aaaaagaaat
     4201 gccttatgtg aacaaagtgg tctcgctgga cgaatatccg gatcttgccc agattatgaa
     4261 agattttttc aaggatgatt cattgtcttt cgctaaactc gattatcaca atagaatgtt
     4321 gactgggaac ccatattctt cctatgtcaa aataagcgaa ggttgcgata atcgttgtgc
     4381 ttattgcgcg ataccgatga tccgcggaca attccgctcg cgtccataca aagaaatcat
     4441 cgacgaatgc aggttgctaa taaaaaacgg tgccaaagaa atcacattga tcggtcagga
     4501 tacaacgaaa tacggaaccg acctaaatga caatgttacc ctggcaagcc tgatgaaaga
     4561 gatcgctgcc atcgaaaatg ttttcttggt ccgggttttg tatctttatc ccgatgaagt
     4621 gacggatgaa ttgattgagg caatggtttc gaatgaaaag atcgccccct atttcgattt
     4681 gccgatccag catatatcga ccccgcttct gaaactaatg aacagaagag gggacgagaa
     4741 cgacattcgt gccttgttga agaaaatcaa agataaaaag agcgatgcga tcatcagaac
     4801 cactttgatt gtgggctttc cgggagagac cgatgccgat tattccaaac tggcggattt
     4861 tgtctttgaa tcgggtttcg accggcttgg cgccttcatg tattcgaaag aagaaggcac
     4921 caaagcattt tccatggaga acatggtttt gccggaagtc atgcaaaacc gttatgataa
     4981 catcatgcgt atccagcgac gcgtttctcg aaaacaaaat cagaaacaag tgggactaat
     5041 ccataaaacg ttagtggaaa attacgatcc ggaaaccgga ttctactacg ggcgtagcta
     5101 tgccttcgcc cctgacgatg tggatggtta catcgtattc aaatccaaac gcgagttgaa
     5161 aatgggcgag gtagttgatg tgctgatcaa ggaatgtatc ggttacgatc taatcggcga
     5221 tgaaatcgga tgaggaggac aaaacaatgg aattcatctt actgttacta aaaggctttg
     5281 tcatcggaat cgcctttatc attccaggtg tttccggggg aacagtagct ttctatttgg
     5341 gtatttatga ccggatcatc cacgccatcg ggcatctctt ttccgaattt aagaaaagca
     5401 tgtcaatctt gatccccgta gctctcggca tcatgatttc cgttgtgatg ttggcaaaac
     5461 tcatggcttg gcttttaaat gtcaattcat tcatgacaat gctctttttc atcggtttgt
     5521 tactgggggg cattcccgga ctgatcggac aaatggatga caaaccgttc aaaaagacca
     5581 ggacgttgat tccgatgatt gttggcttcg ttttggttat ggtcttactg gttgcagaga
     5641 gaaaagcttc cggtgacgca gtagcttcaa tcgacatttc tttcgccaat ttctggttgc
     5701 tttttttact cggagccatt tcggcgatca cgatgatcgt tcccggcata tcgggatcgg
     5761 cgcttctcat ggttcttggt tattatacgg ccattgtcac cggagttttc aaaaacttgt
     5821 ttgattacgc aagtttcgga tataacatct atgttatttt gtcctatgga ttcggagcgg
     5881 tggtcggagt attgcttttc tctcgtccgc ttgattatct gcttaccaat caccgccggg
     5941 aaaccctggc agccatcatc ggtttcgttg cggcttctgt aatcatgatt ttcctggaaa
     6001 tacgcgatcc agcaaccaat gcggaatttg tcgaacaaac gccggtttat ctcgactttt
     6061 ggaattttgc tcgagcgaac atcggatcga ttgtcggtgg aattctgctt ggagtgggtg
     6121 gttttttagc tgcgtatttc atgataagac caaaaaaagc tgatcccgaa gatgtcggtt
     6181 aagcggatcg aagccaagag cttcatgaac aaatcggaat tggatggcga tttgacctgc
     6241 aatccctatt acggttgttc acatcactgt atttattgtt atgccaaata tatcatgcaa
     6301 ggaccggtga catccaaaaa ttgggatgaa gacatccttg tcaaggaatt ttccaattat
     6361 gatattccaa agggaactgg tccggccagg ttgcttttat cgactgctac cgatccctac
     6421 caaccgttgg aaatgaaaga aaaagtgaca cgaaagattt tggaagcgat tgttgacagc
     6481 gatctccacg tttccattct caccaagtcg aaacatgtta ttagagatat cgatcttttc
     6541 aaacggatga aagatgtcag tgtttctttc agcatcgccc ttgacgatga aatgtcagcg
     6601 attatcgaac ccggggcttc tcgtccgtcg gagcggatcg aggccttgaa gattttgcac
     6661 ggcgaaaaca tcagaaccgg agttctgatg gctccgattc ttccgtatct ttcggatgtg
     6721 ttttcgatca tcgacaagat caggggatat gccgacagcg tgatgctcga taccctgaat
     6781 ctcaaaaacc cggaacacat ccgccatgtc atggctttca tcgccacaag atttccggag
     6841 tattatgaag gttatcggaa gatattcatc aaaaaggaca aaacgtatta tcaaaagctg
     6901 aaagaagaaa ttcttgcctg tgaaagtgaa acgccgtttg gcttgaagca tatttataaa
     6961 taggatggtg aaaaatgttc acgttagatg atatcaggcc atacgagaat caaatcgtcg
     7021 cctggagaag aaaaatccac agttggccgg aaaccggatt tgataccata aagacccatg
     7081 attatatccg tacggagctg gaaaatgaag aaatcgaagt ccatactcat gtgggaacca
     7141 attcaattgt cgggataatc agaaacggta cggctccgat tatcggtctt cgtgccgata
     7201 tcgatgccct tcccttgcaa gaggaaacaa gagatcgcgg cttcaagagc cagcgcgacg
     7261 gattcatgca cgcctgcgga cacgacgccc atgcggcaat gcttcttggc gccgctaaat
     7321 tgctttgtaa taacaaaact aagtggtcgg gaaccgtcaa actgattttt caagaagcgg
     7381 aagaaggccc ggatcccggc ggagcctacg gcattgttga atctggactc ttggatgatg
     7441 tggaggattt ctacgctttg catgtctcgc ctgacaatcc agctggtgtt tttgtgacaa
     7501 aatcaggaaa catttttgca tccgttgcca tttttcgcat cactgccaaa ggaaaaggat
     7561 gccatgcggc ttatccggaa atgggtaaaa gtccgatcat tccacttgca aggattgtcg
     7621 aaaagattca ggatatcgtt ccgaataggc caaacaaaaa tgaaaaactc gtggtttccg
     7681 tgactcaagt ccactcaggc accactcata acatcattcc cgaaacagcg atgacggaag
     7741 ggacgatcag ggcctattcg aaagaagcca gggaatatgt cagaaagtcc atggaaaaaa
     7801 tcatcgaaga cgtttctagc ataaccggtg tcgaattcga tttctcgttt cgtgacgtat
     7861 atgaagaaac tgccaatacc gaatcgtctt tcattcgatt tagaaacatg gtagttgctg
     7921 cattcggcaa agaccatttc atggaaatgc ccgaagcgct catgggggga gaagacttct
     7981 tccgttacat aaaaaaacag aaaggctgca tatcctggct tggtacgaga aaggacgaat
     8041 caaccgctta tagtcttcat agtccgtttt tcgatcttga cgaggatgtt ctaaaatacg
     8101 gagccgtcca gttggcaaac ctggcaaccg gcattgaaag gaaggacaaa tgatgtattt
     8161 gattaaaaat gccaatctaa tcggcatgaa ggaccagaat tacgtaaaaa ccgacattct
     8221 gaccgaagga cagaaaatca aagcgatcgg caaactgaat ccggacaagt atccgggatg
     8281 tgtcgtgatt gacgccaaag gcaactatgt gacgccagga atcgtcgacg cccattgcca
     8341 tgtgggcatt tacgaggaag cgatcggatt cgaaggcttg gatgtcaacg aaatgaccaa
     8401 tccggtttta ccggaactta gagcgatcga cggcatcaag cctcaggacg tggcattcaa
     8461 agatgcgctt gaagccggca tcaccactgt ttcgaccggc cccggaagcg ccaatgtaat
     8521 cggaggtact ttctgtattc tgaagaccta cggcaagaca cttgacgaca tgctggtaaa
     8581 agaagaatca gcgatgaaaa tggcccttgg tgaaaatccg aaacgggtct acaaaagtaa
     8641 aaatcaggct ccggaaacca gaatgggttc cgcagcgctg atcagggaag ccttgataaa
     8701 agctagggag tatctagcaa aaaagcaaaa atacgaagaa gatatcagag ccggaaaaga
     8761 agtttcgaaa cccgacttca acatgaaatg ggaatcgctt tcccgggtct tcaagggatt
     8821 gcccgtaaag attcatgccc atcagcaaga cgatatcgcc accgcgatgc ggatcattgg
     8881 cgagttcggg ctggatggaa cgatcgaaca tgccacggaa ggctatttga tccctgagtt
     8941 tttgcatgag cacggccaga gggtcatcat cggtccgaca ctaggatcaa agtcgaaata
     9001 cgaactccgt aacaagagtt tcaaagctgc aaaagttctt ttcgacaatc agatcaaatt
     9061 cgcgatcatg accgatcatc cggtcatcaa tctggcgaat gccctgactc aggtcggact
     9121 tttcgtccgc gaaggcttgc ctgaacttga aggctttcgc gctgtgacaa tcaacgccgc
     9181 cgagctcatt ggcatcagcg acagggttgg aagcattgaa atcggcaaag atgccgacat
     9241 cgttatttgg aacaatcacc cgcttcatta tctcgccaaa ccggaatatg tgttcgtaaa
     9301 tggcgagatt gttgtcgaaa acaaataaaa aagcaagttt tcacttgctt tcccgatatc
     9361 tttggcggca actttcaata agttcaagcg cgtttgccag gggataataa tcgagaacct
     9421 cgacatggtg ccctggttgc aattctttgt aattacggaa gaacaacttg atttccttca
     9481 attgcagatc cttgatgtca ttgatatcat tcacatcctc gaatctggga tccgcgtcga
     9541 cgactgcaat gattttttcg tcatgatcgc cggaatcgta tagatccaaa aatccgacga
     9601 ttctcgcatc gacaatgcac ccggggaatg tctgggtggt ggcaagcacc agaatgtcga
     9661 gcgggtcgcc gtcttctgcc agtgtttcct cgatgaaacc gtattcggcc ggataggtca
     9721 ttttcgaata cagcacgcga ttaagtttga tccgattttt ctgtttgtcg acctcatatt
     9781 tgttttgggt gcccatcggg atctcgatga cagcttcgac gacgtttttc attctcatta
     9841 ttctccttgt tttatatcac ttgcagggcg gaaaaaccgc gctgtggtgt ttattttaaa
     9901 tgatagttat gataaaatat ctttaagagg tgttatggat gaatcttcat gaactgtttc
     9961 gctttcaagc cggatggctc ttaagcaact ggtggtggct tttaccgtcg tttcttgccg
    10021 tattgatgat agtattgtat ctcgccaatg ttttgaagaa ggctagcgaa cataaaccgg
    10081 agattgatat ggccaagctt gaccgaatca tcgccaaact tggtggcctg gaaaatatct
    10141 tggaaacgtc aggcgacgga aatcgtttga aattcaccgt aaaggaattg aagaaatgcg
    10201 atcttgtggc catcaaggaa gatggcgtcc aaggcatttt cgtagccggt aaacaggtta
    10261 aattccaatt gccggaaatg tcggaagctt tattgaaccg gattgaagaa ggcaagaagg
    10321 aggcgacaaa atgatcgaga ataaatacaa gattacttat ccatacggac ttcatgcccg
    10381 taccggcacg atgctggtgg caaaagccaa cggcttcaaa tcggaaatgg tgattgaatt
    10441 cgatgatagg aaggtaaatc tgaaatcgat catgggtgtg ctttccctgg ccgtccctgc
    10501 caacaggtct ttcaggatca tgatcaccgg gtcagacgaa gaagaggcat cgcaggcgat
    10561 cgccagagca atcgctgata tcaacgaaat gaaatgattc aaacgtattg gccatcaaag
    10621 atggcctttt ttttatcttt tacgcgagag accgagtttg tatttgcatt tctccacttt
    10681 tttgaaagca tattcgccgg caatctttct tccgttatcc agcatttcgt ccagttcgga
    10741 ggaattgatg atggtataga aacgctcctg gaacggtttc aggacagatg tgacgacacc
    10801 gacaaggtcc tctttgaaga ttttgtaatt gcacccggtg tactttaaaa ctatctcatc
    10861 cgagcttaat ccggacaaag aagcataaat atcgatcaaa ttcgccaaac caggttggtt
    10921 ttccggatca tatcttacga tgccgataga atcggtgacg gcggatcgaa tttttcttgc
    10981 gatcgtcgct tcgtcatcca aaagggctat gtatcccttg tccgactcgt cggacttgct
    11041 cattttctta aggggatttt gaaggtccat gatccttgcg cccaccttcg gaatcaaagg
    11101 ctcgggaacc cggaagaaat caccgaaacg gttgttgaag cgaatggcga tgtttctcgt
    11161 gatttccaca tgctgtttct gatcctctcc taccgggaca tagtcggcgt cgtataggag
    11221 gatatcggct gccatcagga ccgggtaggt gaaaagcgct gaagtgattc cggtctcctg
    11281 cttggcggcc ttgtccttga actgggtcat tctttccaat tctcccatat aggtgaagca
    11341 ttgcaaaaga tatccgagtt cggcgtgttc cttgatttcg gattggttga ataaaacggt
    11401 tttctttgga tccattccgg ccgccagata agcggctgcg atcattttcg agcgtttgcg
    11461 gagttcggcc ggatcctgtt ctgtcgttat ggcatggaga ttggcgatga aaagatagaa
    11521 ttccgtatcc tcaagttcat tttggattcg aacgaattcc ttgatcgccc ctatgtaatt
    11581 gccaagcgta agaatacctg taggtttaat tcccgataac atacgtttca tgtcagattc
    11641 ctcctttata aagaaaaatc gcccttgtat aaggacgatt ttatcgcggt gccaccttta
    11701 ttccggccat atgaccggcg ctttcatccc ttaacgcggg caacggatgt tattaacatc
    11761 actcctcggt ccattcaccc tgtcttctgt ccggctcgca ccagccgccg gatcgcttca
    11821 tttcagaaac atggttacta ttccgattct tcgtttggaa atattatatc atcatttttc
    11881 ggcgatggca acctccaatt cgccgacaag ttcttccatt cttctaacga ccgccaagaa
    11941 ggcgtcgtct gccgtcagat cgactccggc tgtctgcaac tggttgaccg gataatcgct
    12001 accgccggat ttaagcaagc cgatgtagtt ttcaaatgct cccggaacgc cgtttttgac
    12061 attttcgtaa agtttcaacg atgcggcgaa actggttgcg tattgataaa cgtagaacgg
    12121 ggtgtagaag agatgcggaa tataagccca aacatattgt ttgacttcct ctttgacaat
    12181 gtcaagtccg tagaattcct tgtatagatc aatcatgata tctgataaga catcggcagt
    12241 gatggcttcg ccgttctccg ctttctcatg ggcttcggcc tcatagatcg caaacagcgt
    12301 ctggcggtag aatgtcgaca tgatatcgtc gatcgctctt tgcaatagca tgattttctc
    12361 gttcttcgtg gctttgcttt cacgaatgaa gtaatcgaga agattgtgtt cgttgaaggt
    12421 ggaagcgact tcggccacaa agatcgtgta atcttgaagc gccgaaggtt gggtttccgc
    12481 cgagtaaagt gagtgaatcg aatgtccggc ttcgtgggcg accgtgaaca cgtcttccaa
    12541 agtcccggaa taattcagca ggatatacgg atgtgtatct ggcatcgagg atgaataggc
    12601 tcctgttcgc ttcccgggtt gctcgtaaac atcgacgaat ccttctttaa gtacatccat
    12661 agccttgttt ttgaaatcgg ccgggaactt ttccaaagaa gagaaaaaca ggtttcttgc
    12721 ctcgtcgtag ccgtattcct tgtcggatct cgcaagcggg atgaaacggt cgtatgtatg
    12781 gtattcttcc aatccgagat aatccttgcg aattttcagg tatttcttga tgacgccggt
    12841 gttttttctc gccacgttgc ataaggattt gtacacggaa gccggaatgg catccttgaa
    12901 cagatagctt tcaagcgaat tgggatactt tcttgccttc atcatggcga tatcgtaatc
    12961 aagcactaaa cggtagatcg aggcgtaggt atttttgtgg acatcgtaat aggaaaagac
    13021 ggaatcgaag atgtccttgc gttccgacgg actctggctt ttctggatat aggcgcggta
    13081 gttgctattg gtgatctgga cgatgtcacc ggaatccagc atgacatcct ggtgttcgat
    13141 atccgcgacc gacaatgatc cgtaaagatc ttttccggca tcggccagtt tgccgaagcc
    13201 ggccagcagc gattctgatt tatcatctag aatgtgctcc tgacggcgga acagtttttc
    13261 gaaaccaaag cggaattctt tcaattccgg attgttatcg acaaacgaaa gcactttttc
    13321 tctgccgatc gatatcagtt ccggttcttc gaaggaaacg gcctgttgca gttttgcaaa
    13381 agcgatctgc atcagttgga gtctgctggc gttttcggtg ttttttttgt cgagatcgct
    13441 taacaaggcg gcatattgat atgccctcca gccggtcttc tgcaagtctt tctgaagaag
    13501 gaaataaacc ttgaaatcag cttcgtcgcc aagctttcct ttgaaagtgg cgagtttgtc
    13561 gatcaggtcc agggttttgc ggtaagcttt gtcgtatgtt tcgacgttcg ggaacagata
    13621 agtaaggtcc catttcacat tcatataatt cacactcctt gttcagtttc tcgagattat
    13681 tatatcatca tttcaaaatt atgattacaa taatttatct atatggtata atatagggac
    13741 gaaagtgagg ttggaaatat gataactttg tatacaagtt ccagctgctc ttcctgtcgc
    13801 aaggcgaaaa aatggcttga ggaaaaccgc attcccttca aagaaaaaaa cattgtcggc
    13861 atgaaattga cgcgtgacga tatcatcaat atgctgaaat actcggataa cggattcgaa
    13921 gacatcatct cgacccgctc aaaagttttc aaggacaaca atctcgatac cgaggacatg
    13981 cgttttggcg aactggccga tttcatcatc gataatccga ccattctcaa acgcccgatc
    14041 atcatcaatg acaagatcat gcagacggga tataacgagg atgaaatccg cgctttcatt
    14101 ccgaggaagt tccgtgaaaa catcgtttgc tcggaatgcg acgaggattg cgaatacaaa
    14161 caatgcatca aggaagcatt cgaaaaaaac aaaaaatgag aaatcgggaa cctatggttc
    14221 cccttttttt aaccttggat gcccttaagg cattctgaaa gcgttttaat gtgttgctta
    14281 tattgaaaat cgatggaatt tgcggtaaaa tatgattgat gaaatacata aggaggatat
    14341 gaaaatgtct gtaaaagttg caataaacgg atttgggcgt atcggtcgcc tcgcttttcg
    14401 cgaaatgttc gggaaacctg attttgaaat cgttgccatt aacgatctta ccgatgctgt
    14461 tcaacttgct tacttactga gatatgacag cagtcaaggc cgctatgaaa aaaatatttc
    14521 caccgaagga aacatgcttg tagtggaagg cgtcaaagta ccggtgtttg ccgaaaaaga
    14581 tccctcgaat ctaccttgga aggattacaa tgtcgatgtc gtcctcgaat gcacaggaca
    14641 tttcacatca cgtgacaagg cttctttgca tctccaagcc ggagccaaaa aagtcatcat
    14701 cagcgctcct gccaaagaca aggacatccc cactgtcgta ttcggagtca atgaaacgtc
    14761 gcttaccggc gaagaaacca tcatttccgc cgcatcatgc acaacaaact gcctggcacc
    14821 gatcgccaag gttcttgatg acaacttcaa gatcgtcaaa ggattcatga cgacagtcca
    14881 tgcctatacc aatgaccagg ccaccttgga tattccgcat ccgaagggaa tctatgcccg
    14941 ccgcggccgc gctgccgctg cgaacatcgt tccgacgacg accggagctg ccaaagccgt
    15001 aggattggtt ttaccgcagc tcaagggcaa acttgacggt atcgccctcc gcgttcccgt
    15061 tatcaccgga tcggttgtcg accttgttgt cgaactggga aaacctgtta ccaaggaaga
    15121 agtcaatctg gctgtcaaga acgccgcttc cgacactatc ggatataccg aagatccaat
    15181 cgtatcgagc gatgtcatcg gtattaccca aggaacgatg ttcgatgccc tcagcaccac
    15241 tatcttggaa gtcgacggca agcagatggt aaaagtgatc acctggtacg ataacgagta
    15301 ttcgttcacc gcccagatga ttcgcactct caaatatctc gctgaacttt aataggaaac
    15361 aatctgcgag catccttcgg gatgctcttt ttgtcaataa ggagatactt agatggaatg
    15421 gctttacgca ttgttggaca aatttgaatt attcatatcg agcaacgtat ggtttgcgcc
    15481 gttgtcggct tttatcctac ctttcatcga ggctttgata ccttcgttgc cactatccgt
    15541 ccttgttgca ttcaatctca atatcatgca acaggtttac ggagaaatcg tcggaaccgg
    15601 gctcaccgtc tttttgtcgg tcttgggatc ttttgcgggc atgtttctca ttttcggatt
    15661 gatcaggttg accctggaac cttattttgc ccgcaaggtg caaaaccata agtacgggaa
    15721 aatgtttttg gaaatcgccg aaggatcgaa gtcggttccc atactgcttt tgatgagcaa
    15781 tcctttcttg ccatcttcaa tcatcaacta tgccctgagt ctgacaaaaa tcaaaacggg
    15841 aagatatatt tggcttacta tcgtgtcccg attctttgtc atgatgtttt tgatcttgct
    15901 tggaagtctg ttcgacgtgc aaaatcatct catcaacata gtttggttca cattagctta
    15961 ttccctcctg tttgttttat ggtatatttg gttcaggatc agacgtcgga aaaaggaaaa
    16021 cattaaatga tttcgaaata atgcaaaaag ccgcaatacg gtgttttttg cctgattctg
    16081 tgatataatg aaaaaaagta acgaggtgat acatatggac agagcattct ataaaaaaca
    16141 ccgtgaagat tttcttgcca gaatggacag tcagtcgttc atggttcttt tttccggcaa
    16201 ggctccgcat aagtcggcag accagaagta ccaatacact cctaaccgca atttctttta
    16261 catgaccggt atcggtcggg aaaacatgat tttgcttttg atcaaaggaa aaagcgtcgg
    16321 tcatgagtat ctttttatcg aggaaccaag cgactacatg accaaatggt atggtccgag
    16381 aatgtcgaag gaagaagctg cgaacatcag cggaattgat tcgggatcca tccgctacaa
    16441 gggtgaattt tccggattcc ttgcccaaag cgttctgacc gattcccgta agaatttggc
    16501 cggttcgata aaaatcgctt atttcgatat gtatcgttcc gagcctttgg ccaaaccggt
    16561 aaaccatcct tatttcgaaa ccattatcga taattatccg gaactggaaa tcaaaaacgc
    16621 ctgcgagatc atcgatttgt taagaatggt caaggacgaa tcggaaatag ccgaaataga
    16681 aaaagccatt gcttatacaa aaaaaggttt ggatgcgatt tttaagactg ttcgcccggg
    16741 agtgaatgaa cgcgaactgg aagccgtatt tgcctatgaa atcagaagac agggcagcga
    16801 aggaatttcc ttcgatacca tcgtcgcttc tgggatgaat gcgacatctt tgcattacga
    16861 ggataacaat tgccagatca acggtggcga tctcgtcctt accgatcttg gcgctttgtc
    16921 cgggccctat gccgccgaca ttaccagaac cttcccggca agcagggaat ttaccaccag
    16981 gcagaaaaaa atatacgagc tagttctttc ggtgaacaag gcttgcatcg ccaaagtcca
    17041 tcccggactg gtgatggccg atcttttcag ttttgcaaga gacgaacttg cccgcggact
    17101 gatccgcctc ggcgtgatga gggacatgtc cgaaatcgat aaatattatt atcatagtgt
    17161 cagccattac ctgggcctcg atgtccatga tgtcggcact tatcagttgc ccttggttcc
    17221 cggtgtggtt ttgacaatcg aaccgggcgt ctatatcgaa gaagagggta tcggcatccg
    17281 tatcgaggac gatgttttgg tgacagccaa aggatcgcga aacctgagcg aaagcatccc
    17341 gaaagaaatc gcggaaatcg agcgtctttt ggcaaaatag attttggaag gaaaaaacat
    17401 gggttttctt gacggtaaca tcaggcaata tttgttttac aacgaagaaa attcatattc
    17461 ggtgatcaaa gtcgaaatca tcgacaccga cgaaccggaa ctttcccatt ttgaacccac
    17521 gatcacgatc tgtgggtttt ttcctcgttt ggaactttat cagacataca gatttcacgg
    17581 ggtagtaagc gaacatcaaa aatatggaat ccaatatagt gctgatcggt tcgaacggat
    17641 cagggaacag acagcatcag gaatcatcga ttacctttcg agcgatcttt tcaagggaat
    17701 cgggccaaag acggcggaag cgatcgtcgg aattctcggc attgaagctt tggataaaat
    17761 cgcggatgat ccaagtgtcc tgaatgaaat acccaggatg aacgcggaga agaaagcgat
    17821 tctgtcggaa acgattcgtc gaaaccgggc gatggaatcg gcgcttgttt ggttgtatgg
    17881 attttctata tcgcccaaaa tggcgatgaa gatctatcaa cgcttcggat tcgccacaat
    17941 cgaaaccgtt cgtaacaatc cctattcttt gattgacgaa atcgaaggaa tcggttttcg
    18001 cagggctgac gagatcgccc tgaaaatcgg atttgcttac gacgatccgc taagaatcga
    18061 agcgatcatc gtgttcctgt tgacagaata catgaacaaa tacggcgaca cctatgtgga
    18121 atcagacgaa ctgatgaact atacaatcaa ctatctcaat cagggcgatc agtttcagat
    18181 caaaaccgaa acagtcgata aagaaatcga aaaactcgaa aattccggga aattggttaa
    18241 ggacggcaat cgagtcagtc tgatggcgat ctattactcc gaaaagggga ttgccgattc
    18301 cttgaaagaa tattttgttt ccaaaaacga agcatacgac gaagaaatga ttgccggata
    18361 tcttgaagct tttgaaaaaa gccggcatat ccagtatact gacaaacaga aaaaagcgat
    18421 tatgaaagct ttgaccgaat cgtttacgat catcaccggc ggcccaggaa ccggaaaaac
    18481 aacggtgatc gatgctattt gtcatgttta catgatgatg cgtcaatcca acagtaattt
    18541 cacgtcggca gttaaactgg cagctccgac aggcaaggca gcgaaacggc ttcaggacgc
    18601 aactgatctc gaagctacca ccatccaccg acttttaggc tacgattacg aagggcattt
    18661 tacaaaagac aggcatagtc ctctggaagc cggacttctt gtcatcgacg aagcatcgat
    18721 gatggatgtc attttggcaa accatctttt tcaatcgctc tctgacggta caaaagtagt
    18781 cattgtcggc gatgacaatc agcttccctc ggtgggaccg ggacaggtgt taactgacct
    18841 gatggcatcg gatcagttca cgacggtggg acttgacatg atccatcgtc aggcttccgg
    18901 atcttcgatc gtcaaactgg cgtattccgt tctggaaggc actatcgaag aaagtgtctt
    18961 tcatgacaag gacgatctgt ttttttacgg tgtcccggac gagaaaatcg ccgattttgt
    19021 cgtcggattg atcagacgtt acttgcgaga aggatataac ctgcaagaag atatccagat
    19081 tctggcacca gtttacaaag gcaataacgg aatcgatcgt ttgaacaaga tcgtccagga
    19141 aacattcaac aagcataatg aaggccacaa acttacatat aaagataaaa cctttcggtt
    19201 taacgataag gtcatgcaac tggtgaatca accggaggac ggcatcatga acggagacat
    19261 cggttatgtc tccggcatca tcgaggataa ggaactgttg gtcgattttg gcggcaacac
    19321 cgtgaaatac gatatccggg atttcgacaa cctgactctg gcatattgca taagcatcca
    19381 caagtcgcag ggaagcgaat tcaatgtcgt attgatgccc atgtcgagaa gctatcggat
    19441 aatgctcaag aaaaagctca tctacaccgg tattaccaga gcgaaacaat tgctgtcgat
    19501 catcggagaa aaggatgcct tgcggatagg aatatatggg aatgaaaccc caagaaaaac
    19561 cagattgcgc gattttctgg cattagcggc gaaaagcgga aacgatgatg gcgctttgac
    19621 tatcgaggat tttctttaat tgatgttttc cgattgcatt aaagattgaa atattgtata
    19681 atagccccgt aaatcgatgc gaaagccaag taggaagaaa taaagccccc agagagtccg
    19741 gaatcggtgc gaccggacgg tcgaattttt ccgaagccac tttcaagaga tgttaataac
    19801 atccgcattt ctgcgttatg gaaaaagagt gctaggcaac tagaacaaag gtggtaccgc
    19861 gatcaatcgt cctttacaga taaaggatga ttttcttttt ttagtgtgga ggatac
//
LOCUS       DAOPJL010000023        18784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_3091_length_18784_cov_7.432217, whole genome shotgun sequence.
ACCESSION   DAOPJL010000023 DAOPJL010000000
VERSION     DAOPJL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18784
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_18784_cov_7.432217"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLP02_04105"
     CDS             <1..343
                     /locus_tag="PLP02_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPF42661.1"
                     /translation="KFICHPDIMLFHVKDFDEDIVMESKKIIAKAIETKTPLEINANG
                     IRMGLTKTKEGLRYRYPRLEFWKLVSEMKPIVIVSADAHDPSLLYDEAMNEAYEFARQ
                     LGIEVEEDIMK"
     gene            895..1875
                     /locus_tag="PLP02_04110"
     CDS             895..1875
                     /locus_tag="PLP02_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPF42662.1"
                     /translation="MATVYMIFTGGTITMKMENEHHSVVPALSASDIMSSLLTSFNEC
                     NLKTIEYCQLPSPSITPDIMLGISKLIDELSQQDDVTGFVVVHGTDVLEETAFFLDTV
                     LETKKPVVVTGSMKSSSDLGYDGINNLVSSILVCLSKEAVGKGVLVVLNDQINAASEV
                     TKTNTLSLDTFKSLDYGPLGIVDTHRVIFYRSVNYQRPILHVQDIEENVHLVKVCSGM
                     DDKILDYLIDTLDAKGIVLEALGRGNVPPAMLPGIRKAIAKHVPVIITSRCPSGRVLD
                     TYGYEGGGKHLTTLGCIIAPNLNGQKARILLMIALKKCNDIAQLKQIFGR"
     gene            complement(1925..2473)
                     /gene="ruvA"
                     /locus_tag="PLP02_04115"
     CDS             complement(1925..2473)
                     /gene="ruvA"
                     /locus_tag="PLP02_04115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPF42663.1"
                     /translation="MFSYLKGTITEIRPEYVTLETGDIGYRLFAPNPYQYHIGDIETI
                     YVYQKVAEDEISLYGFKTAAMRDLFLKLISVNGIGPKSAKAILAAGSVEAITAAIESG
                     NAKFLQKFPGIGPKASQQIILDLQGKIELETNIAKTDSLSEVEEALQALGYSQKEIRK
                     ILPKLDAGKSTSDLVRDALKLM"
     gene            complement(2627..3919)
                     /gene="obgE"
                     /locus_tag="PLP02_04120"
     CDS             complement(2627..3919)
                     /gene="obgE"
                     /locus_tag="PLP02_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPF42664.1"
                     /translation="MFIDEVYLDIESGKGGDGIVSFRREKYIPFGGPSGGDGGKGGSV
                     IFEANEGLSTLYDLKYQHLIRAKAGENGKIKNMHGKDSDDVIVKVPVGTMIYERVSNK
                     LLADLTKDKEQAVICKGGKGGRGNAHFATSRNQAPRFQENGEPGEVKSLRIELKLLAD
                     VGIIGFPSVGKSTLISAVTGSKPKIADYPFTTIIPNLGVVDEPGIPPFVLADMPGLIA
                     GASSGAGLGIQFLKHIERTRVLIHMVDMSDLSGRDPYEDYLVIMNELASYRHNLSKRP
                     QILVASKMDADGAVERLASFKKSLDDNIYVYPISTIKRVGLREVLWKAHELLESTPFF
                     PVEDNDAGKEEEAVYYGFNPEEPEIIVKKENEDLYVVSGKDIERLYNRATFQSDESLS
                     RFLSALRKIGIDDYLRKAGAKNGDTIRIGEFEFEFLDQ"
     gene            complement(3939..4421)
                     /locus_tag="PLP02_04125"
     CDS             complement(3939..4421)
                     /locus_tag="PLP02_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42665.1"
                     /translation="MIRNSLKTDPVILEKIARVVARGLNSEGISQWSLTYPSLQDFAN
                     DHEDGSLLIYESEGTILGSVTIKPENDPAYAKIIWEGKNAFVIHRLMVLPEARMKGIG
                     KTLFMAAIEKAKTSKADSVKVDTHPDNLRMQRLILKMGFKRKGYLESINRIGYELILH
                     "
     gene            complement(4423..5583)
                     /locus_tag="PLP02_04130"
     CDS             complement(4423..5583)
                     /locus_tag="PLP02_04130"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPF42666.1"
                     /translation="MHEEITSIYRDKFGTGENLRFFFSPGRVNLIGEHIDYNGGYVLP
                     VAIGIGNVGAIGFREDDLVSFYSDNFEKNGIISTRLSLLEYRPKDRWANYAKGIVLEF
                     KKRGYDISHGFNIAIKGDLPSGAGLSSSASIELLVAIMLNDCFDFKLDRKELALLSQK
                     VENQYIGVNCGIMDQFVIANGKSGKALFLDTSTLFYEEQDVSLGEYDIVVCNTKKRRG
                     LTDSKYNERRMECDKALAFVNNYYQVKDLCSIPFERFYELEAKFDDPVLLKRARHAVT
                     ENARVLKASNLLTQKDIAGFASLLNESHRSLKNDYEVSCPELDYMVDLAISFGSIGSR
                     MTGAGFGGSTVNIVKHRDFNDFSVKVKENYLFRFGVIPDILIANPSDGTKEI"
     gene            complement(5678..5965)
                     /gene="rpmA"
                     /locus_tag="PLP02_04135"
     CDS             complement(5678..5965)
                     /gene="rpmA"
                     /locus_tag="PLP02_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPF42667.1"
                     /translation="MVLHLNILECMASKKGVGSTKNGRDSAGRRLGAKLSDGQYCTAG
                     SIIYRQRGTKIHPGENVGIGKDDTLFAMIDGIVKYERVGRDRKQASVYPQA"
     gene            complement(5993..6304)
                     /gene="rplU"
                     /locus_tag="PLP02_04140"
     CDS             complement(5993..6304)
                     /gene="rplU"
                     /locus_tag="PLP02_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPF42668.1"
                     /translation="MYAVIETGGKQLRVEVGQEIFVEKLDVNAEETYTFDKVLMVGGE
                     KTKIGKPYVKGAIVTAKVVKNGKGPKIIVFKYEPKKHYHRKNGHRQPYTKLTVTDIVL
                     N"
     gene            6535..7506
                     /locus_tag="PLP02_04145"
     CDS             6535..7506
                     /locus_tag="PLP02_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF42669.1"
                     /translation="MDPWVYVVIGLLALIAIYLTFSYFAYRKFFMVRSLPEIQFVDHD
                     SEFFRPSHEWYLKAPKEDVSIRAYDGTLLTGVFLPSIDEKSLLTAIVMHGYRSRATDM
                     IVIAKLYNDMGFKVLMVNQRAHGDSEGNFTTFGQYEKLDLKKWILFALRNYGSTDSIL
                     LHGASMGAATVLMASKLELPDNIKLIVADSSYTNLRALYFAVAKPKITRIFYPGIDFF
                     VWKKHHFLMGEVSPKKAVSDGRIPIFLWHGKADEMVPYEMAGELLESSKSPFKELYSV
                     ENALHTQSYVIDRPGVEKRLSEIVAMLFPTYKNVKAKKEKTSQNKSA"
     gene            complement(7542..9170)
                     /locus_tag="PLP02_04150"
     CDS             complement(7542..9170)
                     /locus_tag="PLP02_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPF42670.1"
                     /translation="MKKAWYKEGVVYQIYPRSYQDSNNDGIGDLRGIINRVPYIKRLG
                     ADIVWLSPVYKSPNDDNGYDISDYRDIQPEFGTMDDMKELIAKLHGAGIKLVMDLVVN
                     HTSDEHEWFKHSVKNDPVYKDYYHWKKEKGNWSSFFGGDAWTYNEERQEYYLHLFSKK
                     QPDLNWDNPAVREEVKNILKFWLDLGVDGFRCDVINIIAKTDGDPDGKKRIALTGREH
                     YLNIPKIHEYLGELRDDVLSKYDCFTVGESVLITPKIALEYIADDKRELNMVFQFDHM
                     GADNHFVKWFMKPFHPINLKKPLSKWQNALKGKGWNTLYLENHDQPRSISRFGNKQFY
                     QESAKLLATMLYFQQGTPFIYQGQEIGMENIAFDNLDDYQDIETKNIYKTGRKMGLSH
                     KRMMKKIKMMSRDNARTPMQWDDTLYAGFSDAIPWIKVNSDHNAINVATNEKNKDSIL
                     SYYKEIISLRKKYPIIVYGDYEDIDFNNKRMYAYKRTIDNESLYVFCNFSAKPLKYDL
                     TGMKGFECLLSNYKEYGDMLLLRPYESRVYYKKA"
     gene            complement(9167..11560)
                     /locus_tag="PLP02_04155"
     CDS             complement(9167..11560)
                     /locus_tag="PLP02_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPF42671.1"
                     /translation="MDIKKILQELTIEEKCSLLVGKDGWHTVPFEKLGIPSIMVADGP
                     HGLRKEQNNPETGKMEIVKAVCFPPATTVACSFDPEISKRMGSAIGKECRKENVHVLL
                     GPGVNIKRNPLCGRNFEYYSEDPVVSGKIASGFIQGLNKEKVGACVKHYAFNSQESYR
                     MVSSSILDERAKNEIYYRSFTEAIKAKPAMVMCSYNRVDDIYASENKELLDDTLRNKF
                     GFDKVIVSDWGAVKNRVNALKATLDLEMPVFKPSVGMLIEAYENQIITEAEIDKSVER
                     LLKLIDEKKDNLPLEVDFEKNHLESKKIAEESMVLLKNEESILPLKTSDNILIVGALA
                     KNVRYQGGGSSHINSYKVDNITDCMPNEVVWEYAEGYAITGDGQNDSLAKEAIEKAKG
                     KDKIVFVLGLPDNYETEGMDRINLNIPKGQKLLLEKMATVNENIVVVLLTGSPVRMPW
                     LSKCKGLLLAGLGGEAVGMAVADILYGKTSPSGRLAETYALELESIPSQRRFAKGNGQ
                     VYYQESIYVGYRYFETADRPVLFPFGYGKSYTEFQYENLHLSSDKLAIGEKLSVEVTV
                     RNTGKVKGKEVVQLYVGNPPSKIFRAVRELKDFAKIELKPGEEKIVSFTLDQDAFSYY
                     NTDIHDFVPLSGTYSIEIRKNAHEGILSSEVLFSTKNDIPVPEAYAKAKSYFAENGLT
                     MTDDDFSLLTGNPLEKERIRRRRPYNLDDSINDLAHTLIGKIIRRKIIKEALKTLDNP
                     TINEMAGIENTICENSLRIISVFSGDKITLNTAKAILALCNNNFRKAARLFKGSAGK"
     gene            complement(11708..11783)
                     /locus_tag="PLP02_04160"
     tRNA            complement(11708..11783)
                     /locus_tag="PLP02_04160"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11748..11750),aa:Arg,seq:ccg)
     gene            complement(11828..11904)
                     /locus_tag="PLP02_04165"
     tRNA            complement(11828..11904)
                     /locus_tag="PLP02_04165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11868..11870),aa:Arg,seq:cct)
     gene            complement(12019..12095)
                     /locus_tag="PLP02_04170"
     tRNA            complement(12019..12095)
                     /locus_tag="PLP02_04170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12059..12061),aa:Arg,seq:tct)
     gene            complement(12211..15087)
                     /locus_tag="PLP02_04175"
     CDS             complement(12211..15087)
                     /locus_tag="PLP02_04175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF42672.1"
                     /translation="MSVKDILIVMGIFLVLSLFLIVMLFLSHKRHIAIMKKTGLAENY
                     LVSHYLDNTPGKIRFSRAILLKAYISICEEITLTDEAKNGIQDDFIRRGIIRMLIRRS
                     NSIFVSVRKKAIASLYYFDTPEIKKTLLKILEREKKEHVKIYVINSLKNKIDQQILQA
                     FVDSIIGSRRFYQRRLIGILNHYLLHVEAHLANIFRRQEMEIKEVFVDLSNIYYREEF
                     RDILTDELKAIEKYLAGEYNITYTPLRRERVMRLYYGILSALAFVYGTPLDDIRYLDS
                     EDSEVVRIASDSFCNRESITDIMTLINLSNGTLSDDYRSHAILKITENNPLLIKDLLE
                     MVPKIAKPEQRLMLAHVFSHRVDYLLLRLLRENEKQLSDIIQLMAENGYTADFIDFLN
                     KNRDKSIDASMKKMLQPIMAENEIFAKEMSEYLSDDKRKYFGLAKPPRQPRVKKIIPE
                     VRKTKWLLTLLIVAIVLCPVIFVLTHLNTVFNESFNDIIKDYVRSLNIWFIGYYFLAN
                     CIYFFLALLSYVGDTRQRVLWNAKNKSMLFEKDMLPSISIIAPAYNEELSIIESVHSL
                     LNLDYPSFEVIVVNDGSKDKTLEKLIDHFQLERKNVLSKPLINTKPVRSIYVNSQLPN
                     LVVVDKENGGKADALNVGINLSHSEYVCGIDADSLLERDSLMNLMSTMLDHDEITLAL
                     GGAIVPVNGFIVDKGQIEAKLLPKSLLARLQVIEYLRAFNISRTGFAELRSLLIVSGA
                     FGLFEKRILIEVGGYLTESSLNKDTVGEDMELVVRITRRACLDKLNYRIGYSGTARCY
                     TEVPEDGKTFFRQRNRWQRGLIDILSYHRKMIMNPKYRLAGFVAMPYFFIFEMLGPLF
                     EIQAYAAVLLGILFGFLSIEIILLLLLVTIVMGIFLSLLSLCISEKVEPTFKPKDIIR
                     LLVMAVIENFGWRQYIALYRVKGFFSSLKENNAWGEMNRVGFRK"
     gene            complement(15084..17105)
                     /locus_tag="PLP02_04180"
     CDS             complement(15084..17105)
                     /locus_tag="PLP02_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPF42673.1"
                     /translation="MRNSKCSSVFPDFFRIDWIGHESVREKIYFLVDGNLVYLFGLSP
                     RFSFDEIKVHILEQAPTGRIQAVFILDFDCLVAEVLKKLEQGKETFDVILPVRPRGFD
                     VSVFSNLCLIYANETAYSYVSPDEKYFQIIPAFFVNRIYSAMVYSAEDRLLLSNHLFD
                     LYPNTSEATRMQTLLDYHRENIPSSNFLRPLLKEVSTLTIEHLFTSMGWEYGAKEIRT
                     ASEKLETLDFYNYYRNNEKTEADDSEKIIVNLLDQIIAKLRTLFGSETVKETFSSNAA
                     FDPVNFSFSASGKEVFKLWHQAFEQIYLKKGQPWLDVLYPLVSKFVETYHVAFPNIYT
                     ALSEREAGKISLLTKEKELLEKQLTDTKDKLESAKQKLLVDKATGLYNELFLYEYLTQ
                     EIKRILDKESKLGDITLIYVCLDNILEINSKYSKASGDEALASFAYLIKQEISSEDLI
                     FKQNGPGFIIMSGDMKKEDAFCLISHIQASVIASETFIEPVTASASLVNLSEFYGSVS
                     PDVIARGMLSSGDHRIKLARQKGPSSVIDSVMEKEEEFVYGSVLVVSDEGIAGKLMNA
                     FFSQANFECIIATDGQKALEIAKNRKFDAIIADKNTPKIDGISLKGQLNESFFTAQVP
                     FILTTYDKSVDTIRRANRAGVDYVIQKPLIFEEILGLIQRHLKRNGDRP"
     gene            complement(17399..17887)
                     /gene="coaD"
                     /locus_tag="PLP02_04185"
     CDS             complement(17399..17887)
                     /gene="coaD"
                     /locus_tag="PLP02_04185"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPF42674.1"
                     /translation="MKVGYYPGSFDPITNGHLDIIERGAKLFDKLFVAVSINPSKRTL
                     FTSDERFELVKEVLKNYKNIEVVKSDILTVEHCRLLGASHILRGLRAVTDYDYEFQLT
                     NFNRKLAPDIDTAFLMTEGQYSYLSSSSVRELAEFGGDVSKFVPPIVKKAIDKKMASV
                     SK"
     gene            complement(17889..18455)
                     /gene="rsmD"
                     /locus_tag="PLP02_04190"
     CDS             complement(17889..18455)
                     /gene="rsmD"
                     /locus_tag="PLP02_04190"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPF42675.1"
                     /translation="MVIEVLRIVSGKWKHLLINEVDRNNTRPTTDRNREMLFNVIGQY
                     FDGGMALDLFAGSGALGIEAMSRGIESCTFVDNDMTACKTIDNNLKKLNVSSNNKKVV
                     KSDGIEFLKNTNSSYDLILADPPYRMEIWNEIVEIVFKRNLLNQEGILVIELPISGSI
                     EERHGSLELIKTKDVGISKFVFYRHKEV"
     gene            complement(18520..>18784)
                     /locus_tag="PLP02_04195"
     CDS             complement(18520..>18784)
                     /locus_tag="PLP02_04195"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HPF42676.1"
                     /translation="LRRFVTELADKENIPYQLSYLNRGGTDAGKIHLTKSGCPSIAIC
                     LAARYIHSHTTIIHEDDYDNAVLLVEKLISALDNEIVNQITFE"
BASE COUNT         4954 a         4230 c         4042 g         5558 t
ORIGIN      
        1 caaattcatt tgccatccgg acatcatgtt atttcatgtt aaggattttg acgaagacat
       61 tgttatggaa tcgaagaaga ttatcgccaa ggccatcgaa acgaaaactc cgttagaaat
      121 caacgccaac gggatcagga tgggactgac aaaaaccaaa gaaggattaa gatacagata
      181 tcctcgtctc gagttctgga aacttgttag cgagatgaaa ccgatagtca tcgtttccgc
      241 cgatgctcat gacccatcgc tcctctatga tgaagctatg aacgaggcat acgaatttgc
      301 aaggcaacta gggatagaag tagaagagga cataatgaaa tgaaaaaagc atcgccatca
      361 tggcgatgtt ttttgttttt atgaattcgt catcaggccg tattttgcca taagacgtat
      421 atataatatg gcaagcgcca aaaaaaggac gaaattcggg caattttcga gcaggttttt
      481 gtaagaagca aaaacttcaa aatcattact tacaattatg ctcatgacgc aaaaaagaat
      541 atgattgttc aagaagaaca agctctaatc attcaaatca tcgtttcatt cgagaaaaaa
      601 caagcaattc gggggataaa tatccctctt ttctaatatc tgttttagtt cctataatat
      661 atattatgtt aacatataag taaccgctat ctttggcgat gcgttaaaac attgtggata
      721 gtgtttaaaa cgcatcaatt gctttttctg caaaatacgg gcctcgaaaa cgaccgtttc
      781 gaaaacatga ctgaaatgtc atggacaaac caccctgatt ttgatttgga taggcatcat
      841 tttctttctt atatgatata atggttttgc aatggaaagg ataatgatat atatatggcc
      901 actgtgtaca tgatattcac aggcgggact attactatga agatggaaaa cgaacaccat
      961 tccgtcgttc ctgctttgtc ggcttccgac attatgtctt ctttgcttac ctcgttcaac
     1021 gaatgcaatt tgaaaacgat cgagtattgc caactcccgt ctccctcgat taccccggat
     1081 atcatgttgg gtatttccaa gctgattgac gaactttctc aacaagatga cgtcacagga
     1141 tttgtcgtcg ttcatgggac cgatgttttg gaagagaccg ccttttttct agataccgtt
     1201 ctcgaaacaa aaaaaccggt cgttgtgacc ggttcgatga agtctagttc cgaccttggc
     1261 tacgacggaa taaacaatct tgtctcgtcg attttggtat gcctttccaa agaagccgtc
     1321 ggcaaaggcg tgttggttgt ccttaatgat cagatcaatg ctgcaagtga ggtaacgaag
     1381 acaaacacat tatcgcttga tacttttaaa tctttggact atggtccctt ggggatcgtc
     1441 gatacacaca gagttatttt ttaccggtca gtgaattatc aacgtccgat tctccatgtg
     1501 caggatattg aggaaaacgt tcatctggtg aaagtttgtt ccggcatgga tgacaagatt
     1561 ctcgattacc ttatcgatac tttggatgcc aaaggcatcg ttctcgaagc tctcggtagg
     1621 ggtaatgtcc cgccggcaat gctgccgggg attaggaaag cgattgccaa gcatgttcct
     1681 gtcatcatca cttcgcgctg cccttccggt cgcgtcctcg atacttacgg atatgaaggc
     1741 ggcggaaaac atctgacgac actcggatgc atcatcgctc cgaatcttaa cggtcaaaaa
     1801 gccagaatac ttttaatgat agctttgaaa aaatgcaacg acatcgcaca gcttaaacag
     1861 atattcggta gataacacat tctctatata agaaaaaacg tccggtttcc ggacgttttt
     1921 gtatttacat cagtttgaga gcatctcgta ccaaatcgga agttgacttt cctgcatcca
     1981 atttaggcaa tatttttctg atttctttct gactataacc gagtgcctgc aaagcctctt
     2041 cgacctcgga aagcgaatcg gtcttggcga tattcgtttc caactcgatt tttccttgta
     2101 aatcgagaat gatctgttgg ctggcttttg gtccgatacc ggggaatttc tgaaggaatt
     2161 tggcgttgcc ggattcgatg gctgcggtta tcgcttcgac cgacccggcc gctaggatcg
     2221 ccttggcact cttaggtccg attccgttaa cgctgatcaa ttttaaaaac aggtcgcgca
     2281 tggccgcggt tttgaacccg taaagagata tttcgtcttc agctactttt tggtagacat
     2341 aaatcgtttc gatatcaccg atatggtatt gatagggatt cggtgcaaaa agacgatacc
     2401 ctatgtctcc tgtttccaga gtgacatatt ccggtcttat ttcagtgatg gtgcctttca
     2461 ggtaactaaa catggcgttt ctccttcttg atttgataag tgagaaaaac aggcaaaaat
     2521 atacaacaca ataacatcac atacatatag aatatgctca tataaataac ataaacatat
     2581 cacatgcaat tttgaaatat ttaattgcgc caaccgtctt ccattatcac tggtccaaaa
     2641 attcgaattc aaattcgccg attctaatcg tatcgccgtt tttagccccg gcttttctta
     2701 gataatcatc aatgcctatt ttacgtaagg ccgagagaaa gcggctgagc gattcgtctg
     2761 attggaatgt ggcccggttg tacaaacgtt cgatatcctt tccactgacg acatagagat
     2821 cttcattttc tttcttaaca atgatttccg gttcttcggg gttgaagccg tagtaaacgg
     2881 cttcttcttc cttgccggca tcattatcct ctaccgggaa gaaaggcgtg ctttccagca
     2941 gttcgtgggc tttccacaat acttcgcgga gaccaacgcg ttttattgtc gatatcggat
     3001 agacatagat gttgtcatca agcgatttct tgaacgaggc cagacgctct acagctccat
     3061 cggcatccat cttgcttgcc acaaggattt gcggccgttt cgacaaatta tgccgatagc
     3121 tcgcaagctc gttcatgatg acaagataat cctcgtaagg gtctctgccg gaaaggtccg
     3181 acatgtcgac catatggatc agcactcttg ttctttcaat atgcttcaga aattggatcc
     3241 cgagtccggc tccgcttgaa gctccggcga taaggcctgg catgtcggcc aagacgaatg
     3301 gcgggattcc cggttcgtcg acgactccca gattgggaat gatcgtcgtg aagggataat
     3361 cagcgatttt cggtttcgaa ccggtgacgg cagaaatcag ggtcgacttg ccaacggacg
     3421 ggaaaccgat gattccgaca tccgccaaaa gtttcagttc gatccttaaa gacttaactt
     3481 cgcccggttc gccgttttcc tgaaatcgtg gagcctggtt tctcgaagtg gcgaagtggg
     3541 cgtttccccg cccgcctttc ccgcctttgc agattacggc ttgctccttg tcttttgtga
     3601 ggtcagcgag caatttatta gaaacccgtt cgtagatcat ggttcccacc ggaaccttga
     3661 caatcacgtc atcggagtcc ttgccatgca tgttttttat cttgccgttt tctccggctt
     3721 tggcccggat cagatgttga tattttaaat cgtataaagt cgacaggcct tcattcgctt
     3781 cgaaaatgac gctgccgcct tttccgccgt cgccgcccga tggtcctccg aacggtatgt
     3841 atttttcccg tcggaaagag acgattccat cgccgccttt tcccgattcg atatctaaat
     3901 atacttcgtc tataaacata atcaatcctc cggaaacatc agtgcagtat cagttcgtat
     3961 ccgatacggt tgatcgattc caagtatcct tttcttttga atcccatctt tagaatcagt
     4021 ctttgcattc ttaggttgtc gggatgggta tcgactttga cactatcggc tttggatgtt
     4081 ttcgcttttt cgatcgctgc catgaaaagc gttttgccga ttcctttcat cctcgcttcc
     4141 ggcaaaacca ttagccggtg gatgacaaag gcgtttttgc cttcccagat tatctttgca
     4201 tatgccggat cgttttccgg tttgatcgtc accgaaccga ggatagttcc ttcgctttca
     4261 tagatgagca gggaaccgtc ttcatgatcg tttgcgaaat cctgtaacga cggataagtc
     4321 aaagaccact gggaaatacc ttcgctgttg agaccgcgtg ccacgaccct ggcaattttt
     4381 tccagaatca ccggatcggt cttcaaagaa ttcctgatca tttcagatct ctttcgttcc
     4441 gtctgaagga ttggcgatca aaatgtcggg aatcacaccg aaacggaaca gataattttc
     4501 tttgactttt acggaaaaat cattgaaatc acgatgtttg acgatattga ccgtcgagcc
     4561 accgaaaccg gctccggtca tcctggatcc gatcgatccg aacgatatcg ccagatcgac
     4621 catataatca agttcgggac aggacacctc ataatcgttt ttaagcgatc tgtgagactc
     4681 gttgagaagc gatgcaaaac cggcgatgtc cttttgcgta agcaagttgg aagccttgag
     4741 aacccgtgcg ttttccgtca ctgcatgtct tgccctttta agcaataccg gatcatcgaa
     4801 tttcgcttcc agttcgtaaa accgttcgaa aggaatcgaa cataaatctt taacttggta
     4861 ataattgtta acaaacgcca aggctttgtc gcattccatc cgcctttcgt tgtatttgga
     4921 atccgtcagc ccccgtcttt tttttgtgtt gcagacgacg atatcgtatt ctcccagaga
     4981 aacatcctgc tcttcgtaaa aaagcgtgga cgtgtccaaa aacagcgcct tacccgattt
     5041 cccgttggca atgacgaatt ggtccatgat accgcagttg acgccaatgt actggttctc
     5101 gactttctgg ctcagtaggg cgagttcctt cctgtcaagt ttaaaatcaa aacagtcgtt
     5161 aagcatgatt gcgacaagca actcaatgga agcgctggaa gacaaaccgg ctccggaggg
     5221 aagatctccc ttgatggcga tattaaaacc atgactaata tcgtagcctc tttttttgaa
     5281 ttcaaggacg atgcccttgg cataattcgc ccatcggtct ttggggcggt attcgaggag
     5341 agagagtctt gtcgaaatga tgccattttt ttcgaaatta tccgaataaa aagaaacgag
     5401 atcgtcttcc cgaaatccga tcgccccgac gtttccgatc ccgatggcga ctggtaggac
     5461 ataccccccg ttgtaatcga tatgttcgcc gatcaggttg acccgaccag gcgaaaaaaa
     5521 gaaacgcaga ttctcgccgg taccgaattt atcccgataa atagacgtga tttcctcatg
     5581 catatcgatc cgctcctatt cataatgatt atatcttatc ctgcgcatat tttcaataac
     5641 atgaaccgga aagaaaaaaa acaggcagcg cctgttttta tgcttgcgga tataccgaag
     5701 cttgcttccg gtcccgtccg acccgttcgt acttgacgat tccgtcgatc atcgcgaaca
     5761 aggtatcatc cttgccgatt ccgacgtttt ctcccgggtg gattttggtt cctctttgcc
     5821 ggtaaatgat tgaaccggcg gtgcaatact gtccgtcgga caatttggca cccagtctac
     5881 gaccggccga gtcacggccg ttcttagtgg aaccgactcc ttttttggaa gccatgcatt
     5941 cgagtatgtt cagatgcagt accatcgata acacctccag attggttatt gattaattca
     6001 aaacgatgtc ggtgacagtc agcttcgtgt aaggctgacg atgaccgttt ttgcggtgat
     6061 aatgtttttt gggttcgtat ttgaaaacga tgatcttcgg gcctttgccg ttcttaacga
     6121 cctttgccgt cacaatagct cccttgacat agggtttccc gatcttcgtc ttctcgccac
     6181 ccaccattag caccttgtcg aaagtgtagg tttcttcggc gtttacgtcc agcttttcta
     6241 caaaaatttc ctggccgact tcaacgcgca actgttttcc acctgtttcg ataactgcgt
     6301 acatgaatag cacctcctcg tgaataattg agcctcgcca ttacggtgcc ttatggctta
     6361 aaaccgtttc tgtgcggtat gcttgacata cgttggtatt atatcttaaa ggcaatgaga
     6421 agtcaataaa aatcgcttcc gtaaggcgat agatttcctg tcatcggttg tttgcgacaa
     6481 aacgcatttc tgaaagaaaa gatccgtgat ataatatgat atgggtgatg aaaaatggat
     6541 ccttgggtgt atgtggtcat cggtctttta gccttgattg caatctatct gactttttct
     6601 tattttgcat accggaagtt tttcatggtc cgttctttgc cggaaatcca gtttgtcgat
     6661 catgactccg aatttttccg tccgagccac gaatggtatc tcaaagctcc caaggaagat
     6721 gtctcgatcc gggcatacga cggaacactt ctcaccggtg tgtttcttcc cagtattgat
     6781 gaaaaatcgc tcttgacggc gatcgtcatg cacggatacc gttcccgtgc caccgacatg
     6841 atcgtgattg caaaacttta taacgacatg ggtttcaagg tgctcatggt gaaccagcgg
     6901 gcccatggtg acagcgaagg caatttcacc actttcggac aatatgaaaa actcgatctg
     6961 aaaaaatgga ttttatttgc tctccgtaat tacggttcga ctgattcgat tctcctgcac
     7021 ggagcttcga tgggcgccgc cacggtgctc atggcatcta aactggaatt acccgacaac
     7081 atcaaactga tagtggccga ttcgtcatac accaatctgc gtgcgttata ttttgccgta
     7141 gcgaagccga agataaccag gatcttttat ccgggcatcg atttcttcgt gtggaaaaaa
     7201 catcatttcc tgatgggcga ggtaagcccg aaaaaagcgg tctccgacgg acgcattccg
     7261 attttcctct ggcatgggaa agcagacgag atggtcccgt atgaaatggc tggggaattg
     7321 ctcgagagtt ctaaatcgcc gttcaaggaa ctttattcgg tcgagaatgc ccttcacacc
     7381 cagtcttacg tcatagacag accgggagtg gaaaaacgtc tgtcagaaat cgttgccatg
     7441 ctctttccca cttacaagaa tgtcaaggca aaaaaagaaa agactagtca aaacaaaagc
     7501 gcctgatgaa aggcgctttt ttatttgcca aggaaaatga atcaggcttt tttgtaatag
     7561 actcttgatt cgtaaggccg gagtagcaac atgtcgccat attctttgta attggaaaga
     7621 aggcattcga aacctttcat tcccgtaagg tcgtacttaa gcggtttggc cgaaaagttg
     7681 caaaagacgt aaagcgattc attatcaatg gttcttttgt aggcatacat tcttttgttg
     7741 ttgaaatcaa tgtcttcgta atcgccgtag acgattatcg ggtatttttt tctcagtgag
     7801 attatttctt tgtaatagga taggatggaa tccttgtttt tctcgttggt tgcgacattg
     7861 atagcgttat ggtcactgtt caccttgatc catggaatgg catccgaaaa tccggcatat
     7921 aaggtgtcat cccattgcat cggtgtacgg gcgttgtccc gcgacatcat cttgatcttc
     7981 ttcatcatcc gtttgtgcga aaggcccatc tttcttccgg tcttgtaaat gttcttcgtc
     8041 tcgatgtcct ggtaatcgtc cagattatcg aatgcgatgt tctccattcc gatttcctga
     8101 ccctgataga tgaatggcgt accctgctgg aaatacagca tggtcgccag gagtttggcg
     8161 gattcctggt agaattgttt gtttccgaaa cggctgatgc ttcgcggttg gtcatggttt
     8221 tccagataga gagtgttcca gcctttgcct ttcagggcgt tttgccattt ggacagcggc
     8281 tttttcaggt tgatcggatg gaagggtttc atgaaccact tgacgaaatg gttgtcggct
     8341 cccatatggt cgaactgaaa aaccatattc agttcgcgct tatcatcagc gatatattcc
     8401 aaagcaatct tcggtgtgat aagcacgctt tctcctactg tgaagcaatc gtatttggaa
     8461 agcacatcgt ctctgagttc tccgagatat tcatgtattt tcggaatgtt aaggtagtgt
     8521 tcccttcccg ttagtgcgat tcttttcttt ccgtcgggat cgccgtcggt tttggcgatg
     8581 atgttgatga catcacagcg gaaaccgtcg acaccgagat caagccaaaa tttaagaatg
     8641 ttcttgactt cttccctgac ggcggggttg tcccagttta gatcgggttg ttttttggaa
     8701 aaaagatgta gataatattc ttgcctttct tcattatatg tccaggcatc tccgccgaaa
     8761 aaagagctcc agttgccttt ttcttttttc caatgataat aatccttata aactggatcg
     8821 tttttgactg aatgtttgaa ccattcatgt tcgtcggaag tatggttcac aaccaaatcc
     8881 ataaccaatt ttattccagc accgtgcaat ttggcgatca gttctttcat gtcatccatg
     8941 gtgccaaact ccggttggat atcccgataa tcggaaatgt cgtaaccgtt atcgtcattg
     9001 ggcgatttat ataccggact tagccaaacg atgtcagcgc ctagacgctt gatatatggc
     9061 acccggttga ttattcccct taggtctccg attccgtcat tgttggaatc ctgatagcta
     9121 cgaggataga tctgatatac tacgccttct ttataccaag cttttttcat ttgccagcac
     9181 ttcctttgaa aagtctcgct gctttacgga aattgttgtt gcaaagggcc agaatagctt
     9241 tcgccgtatt taaggttatc ttgtcgccgg aaaacacgga aatgattcgg aggctgttct
     9301 cgcagatcgt attctcgatg ccggccatct cgtttatcgt cgggttatca agcgtcttta
     9361 aggcttcctt gatgattttc cgcctgatga tttttccgat caaggtatgg gcgagatcgt
     9421 tgatggaatc gtccagatta tacggacggc gacgtctgat tctttctttc tcgagcgggt
     9481 tgccggtcaa aagcgaaaaa tcgtcgtctg tcattgtcag tccgttttcc gcgaaataac
     9541 ttttggcctt ggcgtaggct tccggtaccg gaatgtcatt cttggtggag aacaggactt
     9601 cggaagatag aattccttca tgggcgtttt tcctgatttc gatcgagtaa gttcccgaca
     9661 aagggacaaa atcgtgaata tcggtgttgt agtaactgaa ggcatcctga tcaagcgtga
     9721 aggaaacaat tttttcttct cccggtttca gttcgatctt ggcaaagtcc ttcaactccc
     9781 ttacggcacg gaatattttc gaaggcggat tgccgacata cagttggacg acttccttgc
     9841 cttttacctt acccgtattt cttacggtga cttcaacgga tagtttttcc ccaatagcca
     9901 atttatctga tgaaagatgg agattctcat actggaattc ggtgtagctc tttccgtaac
     9961 cgaacgggaa aagcaccgga cggtctgcag tttcgaaata gcggtatccg acataaatcg
    10021 attcctgata atatacctgt ccgtttcctt tggcaaacct tctctggctc ggaatgcttt
    10081 ccagttcgag agcgtaggtt tccgccagcc ttccgctggg acttgtcttc ccgtaaagaa
    10141 tatcggctac cgccattccc acggcttcgc cgccaagacc tgccagcaat aagcccttgc
    10201 atttcgatag ccacggcatt ctcaccgggc tgccggtcaa taatactacg acaatgtttt
    10261 cgttaaccgt agccattttt tccaagagaa gtttctgtcc tttgggaata tttaaattga
    10321 tccggtccat tccttcggtt tcgtaattgt cgggaagccc caatacaaag acgatcttgt
    10381 cttttccttt ggccttttct atagcttctt ttgccagact gtcgttctga ccatctcccg
    10441 ttatggcata gccttctgca tactcccaaa cgacttcatt tggcatacag tcggtgatat
    10501 tgtcgacttt gtaggaattg atatggctcg aaccgccgcc ctgataacga acgtttttcg
    10561 caagagctcc gacaatcaag atgttgtcgg atgtcttcaa aggcaaaatg ctctcttcgt
    10621 ttttcaataa aaccatcgac tcttcggcaa tctttttgct ttccagatgg ttcttctcga
    10681 aatcgacttc caaaggaagg ttgtcctttt tctcgtcgat cagtttcaga agtctttcaa
    10741 cggatttgtc tatttcggcc tccgttatga tttgattttc gtatgcttcg atgagcattc
    10801 cgacgcttgg tttaaataca ggcatttcta gatcaagagt agctttcaag gcattcactc
    10861 tatttttaac tgcaccccaa tcggatacga tcaccttgtc gaatccgaat ttatttctca
    10921 gagtgtcgtc aagcaattcc ttgttttcgg aggcgtagat gtcatccacc cggttgtatg
    10981 aacacatgac catggcaggc ttggctttga tggcctcggt aaacgaacga taatagattt
    11041 cgttcttggc tctttcgtcg agaatggacg aggaaaccat ccggtaggat tcttgcgaat
    11101 tgaaggcgta gtgtttgaca caagctccta ctttttcctt attgagcccc tgaatgaagc
    11161 cagacgcaat ctttccggaa acgacaggat cctctgaata atattcgaag ttgcgtccgc
    11221 aaagaggatt ccttttgata ttgactcccg gtcccaataa aacatggaca ttttccttcc
    11281 gacattcctt gcctatagca gaacccatcc gtttcgagat ttccggatca aatgagcatg
    11341 cgacggtcgt tgctggcggg aaacataccg ctttgacgat ttccattttt ccagtttcgg
    11401 gattattctg ttcttttctc aaaccatgag ggccgtccgc aaccatgatc gaaggaattc
    11461 ccagtttttc gaaaggaact gtgtgccatc cgtctttacc gacaagcaaa gaacattttt
    11521 cttcaatcgt cagttcctga agtattttct ttatatccat gtcatgacct ccgaataaac
    11581 attttttgtc ttaaattatt atatcattat tgttcgcttt tgttaaatat caatggtaaa
    11641 atcagcgaaa aataactctg tttgcaaaag aaaaaccaca tcgagggaag tggtttctac
    11701 attcatatgg cgactccgac aggattcgaa cctgcaacat tcagctccgg aggctgacgc
    11761 tctatccgtt gaactacgga gtcatgtaac atatatcttg ccggcaatta tgtccgtatt
    11821 ttcctagtgg cgacccggaa gggattcgaa cccccgaccg acggcttagg aagccgctgc
    11881 tctgtcctgc tgagctaccg ggcccagata acattatatc atcaatgaaa tgttttgaaa
    11941 caaaaatgat gtcttgtcat cgttccaaca atgaaacaaa cagccgggac tatttcgtcc
    12001 cggccgcaaa atcgcaaatg gcgactcgga ggcgattcga acgcctgacc gacggcttag
    12061 aaggccgttg ctctatccag ctgagctacc gagccgtaac gcatgtataa ttataatacg
    12121 gcaccggttg tttgtaaaga aatttagcgg ccgggaagca aaaaaaagac atggacaaaa
    12181 agagcaaatc atctatgaaa cattagattg ctatttgcgg aaaccgaccc ggttcatctc
    12241 tccccaggca ttgttttctt tcaaagacga gaaaaatccc ttgacccggt aaagcgcgat
    12301 gtattgtcgc caaccgaaat tttcgatgac ggccataaca agcagacgga taatgtcctt
    12361 cggcttaaaa gtaggttcga ctttttcact tatgcaaagc gaaagcagag ataagaatat
    12421 ccccatcacg atcgtcacca gcaacaataa cagtatgatt tctatactga ggaaaccgaa
    12481 caatatcccg agcaaaacag cggcatacgc ctggatttca aacaaaggtc cgagcatttc
    12541 aaagataaag aaatatggca tggcgacaaa acccgccaat ctgtacttgg gattcatgat
    12601 catcttgcgg tgataagaaa gaatgtcgat gagtccgcgc tgccagcggt ttctttgacg
    12661 gaaaaatgtt ttcccgtcct cggggacctc cgtgtagcaa cgcgccgtac cgctgtatcc
    12721 gattcggtag ttgagtttgt caagacaggc tcttctcgtg atcctgacca cgagttccat
    12781 gtcctctccc acggtatcct tgttcaagga actttccgtg agatagcctc cgacttcgat
    12841 caaaatcctt ttttcgaaaa gtccgaaggc acccgatacg atcaaaagac ttctaagttc
    12901 ggcaaaaccg gttctagaaa tgttgaaagc gcggaggtat tcgatgacct gaagtctggc
    12961 aagaagcgac tttggcaata gtttggcttc gatctggccc ttgtcgacaa taaatccgtt
    13021 cacggggacg atcgctccgc ccagggccaa agtgatctcg tcatgatcca gcatcgtcga
    13081 catcagattc ataagcgagt ccctttccaa aagagaatcg gcgtcgatac cgcagacata
    13141 ttccgaatga ctgagattga taccgacatt gagtgcatcg gctttacccc cgttttcctt
    13201 gtctacgaca accaaattcg gaagttggga attgacgtat atggagcgga ccggcttcgt
    13261 gttgatcaaa ggtttagaca acacattctt ccgttcgagt tgaaaatgat caatcagttt
    13321 ttcaagcgtt ttgtctttag aaccgtcatt tacaacgata acctcgaaac taggatagtc
    13381 gagatttaaa agcgaatgca cgctttcgat gatcgagagt tcctcgttat aggcgggagc
    13441 gatgatcgat atcgatggca acatatcttt ttcgaagagc atcgatttgt tcttggcatt
    13501 ccataaaacc cgctgtcttg tatctccgac ataagacaga agcgccagaa agaaataaat
    13561 gcagttcgca agaaaataat atccgatgaa ccatatattc aaacttctta cgtaatcttt
    13621 tatgatatca ttgaatgatt cgttaaaaac agtattcaaa tgagtcagga caaatatcac
    13681 agggcataga acgattgcga ctataagaag tgttaaaagc catttggttt ttctgacttc
    13741 gggaattatt tttttaacac gcggctgccg tggtggtttg gcaagaccaa aatacttcct
    13801 tttgtcatcg gaaagatatt ctgacatttc tttggcaaaa atctcgtttt ccgccattat
    13861 tggttgaagc attttcttca tggacgcgtc gattgacttg tccctattct tgttcagaaa
    13921 atcgataaaa tcggccgtat agccgttttc tgccattagt tgtataatat cggacagttg
    13981 cttctcgttt tctctcaaaa gccgtaaaag tagataatcg acccgatgcg aaaacacatg
    14041 tgcgagcatg agcctttgtt ccggtttcgc gatctttggc accatctcga gcaaatcctt
    14101 tataagcaac ggattgtttt cggttatctt cagaatggca tgggaacggt aatcgtccga
    14161 tagagttccg tttgaaagat tgattagcgt catgatatcg gtgatacttt cccggttaca
    14221 aaaagaatcc gaggcgattc ttacgacttc cgaatcctcc gaatccagat atctgatatc
    14281 gtctagcggc gtgccgtaga caaaagccag ggccgaaaga atgccataat aaagccgcat
    14341 gacccgttcg cggcgaagtg gagtgtaagt aatattatat tcgccagcca gatacttctc
    14401 gatcgcttta agctcatcgg tcagaatatc gcggaattct tcgcggtaat atatattgga
    14461 taaatctaca aatacttctt ttatctccat ttcctgacgg cgaaagatat ttgcaagatg
    14521 agcttcaaca tggagaagat aatgattgag aataccgata agccgacgtt ggtaaaagcg
    14581 tcggctgccg atgatgctat cgacaaaagc ctgaaggatc tgttggtcaa ttttgttctt
    14641 tagagaattg atgacataaa tcttgacgtg ctcttttttc tccctttcca ggatcttaag
    14701 taaagttttc ttgatttcgg gagtatcgaa ataatagaga ctggcaatcg ccttttttcg
    14761 tacggaaaca aaaatactat ttgatcttcg gataagcatc cgaatgatgc cgcgtcttat
    14821 gaaatcatcc tggatgccgt tttttgcctc atcagtgagc gtgatttcct cacatatgga
    14881 aatgtatgcc tttaggagaa ttgctcggga gaatctaatt ttacctgggg tattgtcaag
    14941 gtagtggctg acaagataat tctcggccaa tccggttttt ttcataattg cgatatgtct
    15001 cttgtgactt aaaaaaagca tgactatcaa aaaaagggat aagacaagaa aaatccccat
    15061 gactatcagg atgtctttaa cactcatggc ctatccccgt ttcttttcaa atgcctttgg
    15121 attaacccca gaatctcttc aaagatgagc ggtttctgaa tcacatagtc aacaccggct
    15181 cggttggctc ttcttattgt gtcgacggat ttgtcgtagg ttgtcaaaat gaaaggaacc
    15241 tgagcggtaa agaaagactc gttcaactgt cctttgagcg agatcccgtc aatcttcggc
    15301 gtattcttat ccgcgataat ggcatcaaat tttcgatttt tcgcgatctc caaagccttt
    15361 tgtccgtccg tggctatgat acattcgaag ttggcctgtg aaaagaaggc gttcatgagt
    15421 ttgccggcaa taccttcatc cgataccacg aggacactgc cgtaaacgaa ttcctcttct
    15481 ttttccatga cggaatcgat taccgatgaa ggtcctttct gtcttgcaag ctttatcctg
    15541 tgatctcctg acgaaagcat tccccgagct atcacatcgg gtgatacgga tccatagaat
    15601 tctgaaagat ttaccaaaga ggccgatgcc gtcaccggct cgataaaggt ttctgatgca
    15661 atgacagaag cctgaatatg gcttatgaga caaaaggcgt cttctttttt catgtctcca
    15721 gacattataa tgaatccggg accgttctgc ttgaaaatta agtcttcaga agaaatttct
    15781 tgctttatca ggtatgcgaa actggcaagc gcttcgtcgc ccgaggcttt tgaatacttg
    15841 gaattgattt ccaggatatt gtcgagacag acatagatca gagtgatgtc accaagtttt
    15901 gattctttgt cgagaatccg tttgatttcc tgggtaagat attcgtatag gaaaagctcg
    15961 ttatataatc cggtcgcctt gtcaaccagc agtttctgtt tcgcggattc gagtttgtct
    16021 ttagtatcgg tcaactgctt ttcgagtagt tccttttctt ttgtcagtaa agagattttc
    16081 ccagcttctc tttctgaaag agctgtataa atattgggaa aagcgacatg gtaggtctcc
    16141 acaaattttg acaccagcgg ataaagcaca tcaagccaag gctgcccttt tttcagatat
    16201 atctgttcaa atgcctgatg ccataatttg aagacttctt ttccggaagc ggaaaacgaa
    16261 aaattgaccg gatcaaaagc ggcgtttgaa gaaaatgttt ctttgactgt ttccgagccg
    16321 aacagagttc tgagtttggc gatgatctgg tccaatagat tgacaatgat tttctcagaa
    16381 tcatcagctt cggttttttc gttgtttcga taataattgt aaaaatccag agtttccagc
    16441 ttttcagatg ccgtccggat ttctttcgcc ccgtattccc agcccatgga tgtgaataag
    16501 tgctcaatcg tcaaagtcga cacttctttc aaaagcggtc ttaaaaaatt gcttgaggga
    16561 atgttttccc gatggtagtc taacagcgtt tgcattctgg tcgcttcaga ggtgttggga
    16621 tacagatcga acagatgatt tgataacaaa agtctgtcct cggcggaata aaccatggca
    16681 gagtaaatcc ggtttacgaa aaaagccgga ataatctgaa aatacttttc gtccggcgat
    16741 acataagaat atgcggtttc atttgcatag ataagacaaa gattcgaaaa aaccgataca
    16801 tcgaagcctc tcggtctgac gggcaaaatc acatcgaacg tttctttgcc ctgttcgagc
    16861 ttcttgagca cttcggcaac caggcaatcg aaatcgagaa tgaaaactgc ctggatgcga
    16921 ccggtaggag cctgttcgag aatatgtact tttatttcgt caaacgaaaa acgcggacta
    16981 agtccgaaaa gataaacgag atttccgtcc acaagaaaat atattttttc tcgaactgat
    17041 tcatgaccta tccagtcgat ccggaaaaaa tccggaaaaa cactggaaca tttgctgttt
    17101 ctcatagaat ccgcatagtc catgaatatc accgtcctga aaaacattat acattatttt
    17161 tttggtttac gcattagcta aaaaaaaacc aataaaaata aatggttttt aaacatacga
    17221 agtgcctttt tgtcagatac ttgttgaaag aatgaattaa tagaaaaaat taatggaatt
    17281 atcaaaaaaa aacatatgtt ttgcagaaat gatttgttaa aaaaaatctt ccttaagcat
    17341 cctggtaaat agttcgatta ccgtttgctt aatgaagatc aaatatggat aaacagttct
    17401 atttactgac tgaagccatc tttttgtcaa tggccttttt gacaatcgga ggaacaaatt
    17461 tggaaacatc ccctccgaat tcggccagtt cccttacgga tgatgacgag agataggaat
    17521 actgtccttc cgtcatcaga aaagccgtat cgatatccgg agcgagtttg cgattgaaat
    17581 tggtgagttg gaattcgtaa tcgtagtcgg tcactgcccg caatccgcga aggatgtgac
    17641 ttgccccaag caatcggcaa tgttccacag taaggatatc cgatttgacc acttcgatgt
    17701 ttttgtagtt tttcaaaact tcttttacca gttcgaacct ttcatcggat gtgaaaagag
    17761 tccttttact gggattgatg gataccgcga caaacaattt atcgaacagt ttcgctcccc
    17821 tttcgatgat gtcaagatgt ccgtttgtta tcggatcgaa acttccggga taatatccta
    17881 ctttcatttc atacctcctt gtggcgataa aagacaaact tgctgatgcc aacatctttt
    17941 gtttttatga gttcaaggga accatgtctt tcttcgatac tgccgctgat cggtaattca
    18001 ataaccagga ttccttcttg attaagcaaa tttcttttaa aaacgatttc tacgatctca
    18061 ttccatattt ccattctgta cggaggatcc gccaggatca gatcatacga agaattcgta
    18121 ttcttaagga attcgattcc gtctgatttt acaacctttt tattgttaga agaaacgttc
    18181 agctttttca gattgttatc tatagtcttg caggcggtca tgtcgttatc gacaaatgtg
    18241 caactttcga tgccacggct catagcctcg atccccaaag ctccgcttcc cgcaaacaga
    18301 tcgagcgcca ttccgccatc aaaatactgt ccgatgacat taaaaagcat ttccctgttt
    18361 ctgtccgtag tcggtcttgt gttgttcctg tcgacttcat ttatcaacaa gtgtttccat
    18421 tttccggata cgatcctgag cacttcgatc accatctaga attttaacac tgccagccag
    18481 ataaagcaac tcccgacaag agaaaaaggc ctttcggcct cattcaaaag tgatttggtt
    18541 gactatttca ttatcgagcg cagaaatgag cttttccacc aaaaggaccg cgttgtcgta
    18601 atcatcctcg tggatgattg tcgtatgcga atgaatgtat cttgccgcca agcagatcgc
    18661 gatagacgga catccacttt tggtcagatg gattttgccg gcatcagtgc cgccgcgatt
    18721 gagataagac agctgataag gaatattttc cttgtcggcc agttcggtca caaaccttct
    18781 caag
//
LOCUS       DAOPJL010000024        15442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_4023_length_15442_cov_9.576916, whole genome shotgun sequence.
ACCESSION   DAOPJL010000024 DAOPJL010000000
VERSION     DAOPJL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4023_length_15442_cov_9.576916"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1993)
                     /locus_tag="PLP02_04200"
     CDS             complement(<1..1993)
                     /locus_tag="PLP02_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPF42677.1"
                     /translation="MKKHPLATLVTIASAYFAIGVAGFIFMLLANAGPAAVLIYVLSV
                     CLTIFVALMAFFGSRRNAFRMSDLQDKFKRKNLLEKKLVSSEDIALNYMPLGMIIYDE
                     AYQIVWANHAAKDFFSNVLIDRKIAVVHEELAKHVETRETRFILDIYDKAYDIYHYPN
                     NRCIYLFEVTERETAKRKLEERKSVLGVMSLDNFNEATSNLDVQDKSNLQGKFLGAID
                     TWCKNRHIFFVNLRPEKSVIYLTLGQLHELMSEEFQILDTVNEIANQNEVRISLSIGL
                     ACYEDEMFDQLGEFAEEALKLAQGRGGDQVVVNIENQPLKFFGGKSNTVEKRTKITAK
                     INSRAISEFIQKHEKVLIMPHKSTDIDALGASIGLLQMALAQKKDARIVLDFDGIDQT
                     CQKVMNMLSREYVKLMEYFIEPEDALNEITPETLLFVVDHHSPTQSNAPKLLEHTKYL
                     IVIDHHRRIDNLLSDVMLTYMEPYASSSVELVIEMVELYNHEVDIDPFEATIMLAGMM
                     IDTNNFTYRTGVRTFEAAALLKRYGADPFKARLILRESLDHIKTKSNLVNRAKIINNR
                     FAIAMLPPENQTDRVQLARTADELLEIDNIVGAFAIGDIGNDTIAISARSVDKFNVQM
                     IMEQFGGGGHLNNAAAQVENGDVVDIAKKIETILETTFKE"
     gene            complement(2103..2366)
                     /gene="rpsR"
                     /locus_tag="PLP02_04205"
     CDS             complement(2103..2366)
                     /gene="rpsR"
                     /locus_tag="PLP02_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPF42678.1"
                     /translation="MYNSNNSSRPGGNFRGGRKRPRKTCYFTDNKIAYIDFKDFELLK
                     KFISERGKILPRRVTGTKAYYQKHLAVAIKRARFMALLPFVKE"
     gene            complement(2388..2831)
                     /gene="ssb"
                     /locus_tag="PLP02_04210"
     CDS             complement(2388..2831)
                     /gene="ssb"
                     /locus_tag="PLP02_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPF42679.1"
                     /translation="MLNRVVLVGRLVRDPELRYTQNNIAVVSFTLAVNRPFASSNQQN
                     DNTADFISCTAWRKQAENISKYVNKGSMVAVDGRLQTRDYMDEKNNVRRYVTEVVCDS
                     VVFLDSKSQSQDSGNGHNYDASDNNNTKKDDKEDSIDIIEDDLPF"
     gene            complement(2847..3137)
                     /gene="rpsF"
                     /locus_tag="PLP02_04215"
     CDS             complement(2847..3137)
                     /gene="rpsF"
                     /locus_tag="PLP02_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016127597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPF42680.1"
                     /translation="MRKYEIMYIIRPTVEAEARKALIEEMNKILTDQGAADIAVNEWG
                     MRELAYEIDDCKKGYYVVLSLNAEPAATMEADRVMKIKEDVLRHIIISREEK"
     gene            complement(3265..4494)
                     /locus_tag="PLP02_04220"
     CDS             complement(3265..4494)
                     /locus_tag="PLP02_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF42681.1"
                     /translation="MTRLWNRNFSIVTIGSFISALGSAAAGVAFGILVYTETGSPLML
                     AMFFVANVVPRIVASFLAGPYVDRHSRRNIIVTIDYFYSLFFLAMGLVLFTGFFNVWV
                     FTAISALIGTIDTIYQTAFMSLFPETVPKGSHSKAYSIASLIWPLSAAIMAPIAAYFI
                     DNFSNGTAILMIFNAVTFVVTASIELLINVKENLNDSQIEKRQFITDIRESIHYFKHE
                     RGILGIALLFGAFAFVYAVGDLLRMPFFVSSPNFTIQDYSFLISAGAIGRIVGGLVHY
                     VFRLPVEKKFTIAIVVYFTVEVLDATMLFMPYILMITMSFIVGLLGVTSFNIRMAATQ
                     TYVSDNIRGRVNATQQLIWNFGSIAGAVAAGLIAEYSGLDYRIIIMFAAVVSISSIFL
                     IPLRMKDEFKKIYNVEV"
     gene            complement(4532..5251)
                     /locus_tag="PLP02_04225"
     CDS             complement(4532..5251)
                     /locus_tag="PLP02_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPF42682.1"
                     /translation="METKNEENIRFSERYLLFGIDREQIENLIKEHELNYKRVGIRIN
                     DDEGEPTVELSSSEQKYLTKARKRFVEIFSDYLFFPPNRTLAEAFFETLEKRNLQVST
                     AESVTGGMIASRILDQPGASKIMKEAFVVYSNQAKMDLLGINPKTIQKYGVVSEEVAS
                     EMADRLQNVSGANIAIATTGIAGPDGGTENIPVGTVFFGFVITKDKSTERKQFSGDRN
                     EIRKKATAYAMAYVINRLNNK"
     gene            complement(5263..6366)
                     /gene="ychF"
                     /locus_tag="PLP02_04230"
     CDS             complement(5263..6366)
                     /gene="ychF"
                     /locus_tag="PLP02_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004893236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPF42683.1"
                     /translation="MGLTAGIIGLPNVGKSTLFNAITKSKVLAANYPFATIDPNVGVV
                     EVPDDRVDVLTKMYTSRKTIYTTFEFTDVAGLVRGASKGEGLGNQFLSHIRGCDAICQ
                     VVRCFDDPNVVHVEGSIDPLRDMETIMIELIFADLDVIEKRLPKIQKKAEMKADQETA
                     IEYAILLKLKDALESSIPIRAVNLTDEEGKIIKNFSFLTAKPMLYVCNVSESEMADYG
                     ANPQVRKVVERATSEGAATTVISAKIEQELSELSDEDKKDFLSDFGMEESGLSDLIRK
                     SYDLLGLQTFFTAGEKEVRAWTFKKGMKAPECAGVVHSDFEKGFIRAETISYEDLVAC
                     GSETKAKELGKYRLEGKDYIARDGDVFHFRFNL"
     gene            complement(6480..7250)
                     /locus_tag="PLP02_04235"
     CDS             complement(6480..7250)
                     /locus_tag="PLP02_04235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPF42684.1"
                     /translation="MFTDSHAHLNDSLLYPEADEVIRRANLAKVTRIICPGYDLESNK
                     KAIELAERYPAVFAAIGFHPENIGPVTDADYEWLKEHCHHEKVVAIGEIGLDYYWDKS
                     LMEKQKDAFIRQIGIAGDNNLPMIIHMRDATADTLNILMKHKKQSAGGVMHCYSGSVE
                     SAKDFIGLNMFISLGGPVTFKNAKAAKEVAAIIPLEYLLIETDSPYLAPTPYRGKTNE
                     PAYVVRVAEEIAGIRNMPTETIGLITSKNAEKLFAIAG"
     gene            complement(7251..9212)
                     /gene="tkt"
                     /locus_tag="PLP02_04240"
     CDS             complement(7251..9212)
                     /gene="tkt"
                     /locus_tag="PLP02_04240"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPF42685.1"
                     /translation="MKNTILNMKFLAIDMINKANSGHPGIVLDAAPIMYSLFQYHLDG
                     DPKNPDWFNRDRFILSAGHGSALLYSTLFLAGYDIPFGEIQKFRQLGSMTPGHPEYRH
                     TPGVEATTGPLGQGIGMAVGMALAEAYLGATFNRPGFPVVNHDTYTLCGDGDLQEGVA
                     MEALSLAGHLRLNKLTVLFDSNDIQLDGPTSKATSDDIKAKMESMGFFYQKVESSDDV
                     IGLSIAIEKGRQSDKPSFIEIKGIIGEGSAKQGTSAVHGSPIGVEDALRMKLEAGYSL
                     EDFCVLDTSTADFSDKFIKRGQTEKKVWDAMITEYAGKYPEEYKQLTSIMNGECDLEF
                     VKNIGLLENGQKEATRKSIGRLLKTYQENNQALIGGSADLTPSTQVKGINGDFSIENR
                     TGRNINFGVREHAMGAIVNGLTLHGLRGFSGGFFVFSDYMKPAIRLGAIMGIPSLFIF
                     THDSIAVGEDGPTHEPVEQLAAFRSTPNLLVLRPADANETAYAMHLAMETMDRPTVII
                     LTRQDLTVAIQSDYLDFKKGAYIASDIKDYTGIIIASGSELGLAMEAQEKLYKEHQIP
                     VRVVSMPSMETFLGQSEAYQNQILSLPIDKRLAIEMGASGLWYRFAGNVVGIDTFGAS
                     GKAADVLKHFGFTADDIVSKCLKAFGKNK"
     gene            9428..9943
                     /locus_tag="PLP02_04245"
     CDS             9428..9943
                     /locus_tag="PLP02_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42686.1"
                     /translation="MNVLTQRLCIRDFKEDDFDDLWAIFKDKETMKHVRNYTKPETLT
                     FFRSFCLSKTPPGAFAVVLKDIDKVIGYLLFNNIDEKGIYEIGWIFNRMYWREGYAYE
                     AAKAMIKYAFDSLDAHKIVAEAEDTDKSVPLMKKLGMIQEGIFRKHCLTESKTWKDLY
                     WYGILKDDICG"
     gene            complement(10016..11200)
                     /gene="tuf"
                     /locus_tag="PLP02_04250"
     CDS             complement(10016..11200)
                     /gene="tuf"
                     /locus_tag="PLP02_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPF42687.1"
                     /translation="MAKQKFERTKPHLNIGTIGHVDHGKTTLTAAITLVLSKQGLAQV
                     KDYASIDSAPEEKARGITINTAHIEYETKNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGAMPQTREHILLAGQVGVPKMVVYLNKADMVDDPELIELVEMEIRELLDE
                     YGFPGEETPFVVGSALKALEGDEKEQQTLLHLMDVVDEYIPLPVRDINKPFLMPIEDV
                     FTITGRGTVVTGRVDRGTIKVGDPVEIIGIKPTKASVVTGVEMFRKQLDYAQAGDNVG
                     LLLRGVDRDEVQRGQVIAKPKSVTPHTKFTAQVYVLTKDEGGRHTPFFTNYRPQFYFH
                     TTDITGVINLAEGVEMVMPGDNCEMTVELIHPIALEQGTKFSIREGGHTVGAGSVINI
                     IE"
     gene            complement(11311..13380)
                     /gene="fusA"
                     /locus_tag="PLP02_04255"
     CDS             complement(11311..13380)
                     /gene="fusA"
                     /locus_tag="PLP02_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPF42688.1"
                     /translation="MAREFSLERTRNIGIMAHIDAGKTTTTERVLYHTGKIHKMGETH
                     DGASQMDWMEQEQERGITITAAATTAFWKNYRVNIIDTPGHVDFTVEVSRSLRVLDGA
                     VTVLDAQAGVEPQTETVWRQANEYHVPRVVFANKMDKAGADFAHSVESIHNRLGAKAE
                     AIQWPIGAEETFDGIIDLVQMKAFHFDGEPEENYVEIPIPKQLLKIVEQKRTTLIEAV
                     ADFDEELMLTYLDGGEISVALMKQAIRKATLTREFFPVICGSAFKNKGVKLLLDTVID
                     YLPSPVEVPQIRGFNKQGKEVVIGHDDNKEFVALAFKVMTDPFVGRLTFFRVYSGVAE
                     KGSYIINTSKGKKERLGRILQMHANHRTEIDAVYSGDIAAAIGFKDTTTGDTLCSDKI
                     DVVLESMKFPEPVISVAIEPKTRADQDKMGVALSKLAEEDPTFRTYTDKETGQTIISG
                     MGELHLEIIVDRMRREFNVEATVGQPQVSYRETIKQAGDCEGRFIRQTGGHGQYGHVW
                     IKFEPNPGKGYEFVDKIVGGVVPREYIPVVDKGLQEALLGGIVAGYPVLDIKATLFDG
                     SYHEVDSSELAFKIAASLALKQTREKCKPTLLEPIMNVDVVIPDEYIGNVIGDLTSRR
                     GRIEAQETQGKAQKVSAKVPLSQMFGYATALRSNTQGRATHTMQFSHYEECPKSIAEE
                     IIAKRNG"
     gene            complement(13400..13870)
                     /gene="rpsG"
                     /locus_tag="PLP02_04260"
     CDS             complement(13400..13870)
                     /gene="rpsG"
                     /locus_tag="PLP02_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPF42689.1"
                     /translation="MPRKGKVVKRDVLPDPIYNSKLVTKLINGIMLDGKKNVAQNILY
                     RAFENIKTTTGKEPIEVFNEALNNIMPVLEVRSRRIGGQNYQVPGEVRNDRRVTLGLR
                     WLVLYSRLRNEKTMEERLAKEIIDASNGTGASVKKKEDMHKMAEANKAFAHYRF"
     gene            complement(13918..14340)
                     /gene="rpsL"
                     /locus_tag="PLP02_04265"
     CDS             complement(13918..14340)
                     /gene="rpsL"
                     /locus_tag="PLP02_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPF42690.1"
                     /translation="MSTINQLIKQGRHDKVKKSKAPQLNIGFNTLQKRYTDLNSPQKR
                     GVCVRVATMTPKKPNSALRKYARVRLSNGGEVNAYIPGIGHKLQEHSVVLVRGGRVKD
                     LPGVRYHIIRGTLDTTAVQDRKQSRSKYGAKRPAPAKK"
     gene            complement(14508..14891)
                     /locus_tag="PLP02_04270"
     CDS             complement(14508..14891)
                     /locus_tag="PLP02_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42691.1"
                     /translation="METIYDRITLLLKKKNKTRKDLCAATGISYHTLMSLYQRKSENM
                     KLDTVMLIADYLGVTADYLISGTDIGLAVSETGVIPYGDEVSPNMELEVVRIMKRLRP
                     RDKAILLSKAYELEEKEKKEEQEKE"
     gene            15040..15243
                     /locus_tag="PLP02_04275"
     CDS             15040..15243
                     /locus_tag="PLP02_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42692.1"
                     /translation="MVYANLKAEMVRKDISATQMAGLLNISLRLLDERMRCESGFTIR
                     EVKIIMDLFPECSIMDLFTKTPC"
BASE COUNT         3680 a         3606 c         3530 g         4626 t
ORIGIN      
        1 cctccttgaa tgttgtttcg aggattgttt cgatcttttt agcgatgtcg acgacatcgc
       61 cgttttcaac ttgggctgcc gcattgttta ggtggccgcc gccgccgaac tgttccatta
      121 tcatctggac attgaattta tcgacggaac gggcggaaat cgctatcgta tcgttgccga
      181 tatcgccgat agcgaaagcg ccgactatgt tgtcgatttc caataactcg tcggcagtcc
      241 ttgcaagttg gaccctatcg gtttggtttt ccggtggcaa catggcgatg gcgaaacgat
      301 tgttgatgat tttcgcgcgg tttaccaaat ttgattttgt tttgatatga tctagggatt
      361 cacggagaat caatctggct ttgaacggat cggccccgta tcgtttgagt agggcggccg
      421 cttcgaaagt cctgactccg gtccggtagg tgaaattgtt tgtgtcgatc atcattcccg
      481 ccaacatgat agtggcttca aacgggtcga tgtcgacttc atggttgtaa agttctacca
      541 tttcgatgac cagctcgacg gaactggaag catatggctc catgtatgtg agcatcacgt
      601 cggaaagcag attgtcgatc cggcggtgat ggtctatgac gattaaatat ttggtgtgtt
      661 ccaacagttt gggagcgttg gattgggtcg ggctatgatg atcgacgacg aacagaagcg
      721 tttcgggcgt gatctcgttc aaagcgtctt ccggttcgat gaagtattcc atcagtttga
      781 cgtattcccg gctcaacatg ttcatcactt tttggcaggt ttggtcgatc ccatcgaaat
      841 cgagaacgat tctagcgtcc tttttctggg caagagccat ctgcaaaagg ccgatcgagg
      901 ctccgagggc gtcgatatcg gtcgatttgt gcggcatgat cagcaccttt tcatgtttct
      961 ggataaattc ggaaatggcg cgggaattga tctttgcggt gatttttgtc cgtttctcga
     1021 ccgtgttgct cttgcctccg aagaatttca aaggctggtt ttcaatatta acgaccacct
     1081 ggtcgcctcc gcggccttgg gcaagtttga gagcttcttc agcgaattcg ccgagttggt
     1141 cgaacatttc gtcttcgtaa catgccaatc cgatcgacaa tgaaattctg acttcgttct
     1201 gattggcgat ttcattgacg gtgtcgagaa tctgaaattc ttcgctcatg agttcgtgca
     1261 gctgtcccaa agtcagatag ataaccgatt tctcggggcg aaggttaacg aagaatatat
     1321 ggcggttctt acaccaggta tcgatcgctc ccaggaattt tccctgaagg ttcgatttgt
     1381 cctgaacgtc cagattgctg gtggcttcgt tgaagttgtc gagactcatg acaccaagaa
     1441 cgctttttct ctcttccagt tttcttttgg ccgtttccct ttcggtaacc tcgaacaggt
     1501 agatgcaacg gttgttagga taatgataga tatcatatgc tttgtcatag atgtcgagaa
     1561 tgaatctggt ttcgcgagtc tccacgtgtt tggccagttc ctcgtgaacc acggctattt
     1621 tccggtcgat caggacattg gaaaaaaagt ctttggccgc atggttggcc cagacgattt
     1681 gatacgcttc gtcatatatg atcatgccga gaggcatata gttcagggca atgtcttcgc
     1741 tgcttaccag tttcttttcc aaaaggttct tcctcttgaa tttgtcctgg agatcgctca
     1801 ttcgaaatgc atttctgcga ctgccgaaaa aagccatcag agccacaaaa atcgtcaggc
     1861 atacggaaag aacataaatt aatacggcag cagggccggc gtttgctagc aacatgaaga
     1921 tgaaccctgc gacgccgatt gcgaaataag cggatgcgat ggtgacaagt gtcgccagcg
     1981 gatgcttctt cataaacgca cctcgcaaaa aggttttata gtcaaaacaa ttatatcaca
     2041 tcggtaagaa aaaacaaact cgagccagaa gaaaaaaaga ctcgatgagt ctttttagga
     2101 tactattctt tcacaaaggg cagtaaagcc atgaaacggg cccgcttgat ggcgacggcc
     2161 aggtgttttt ggtaatatgc cttggtgccc gtgacccgac ggggaaggat ttttccgcgt
     2221 tcggagatga atttcttcag cagttcaaaa tccttgaaat cgatatatgc gattttgtta
     2281 tcggtaaaat aacaggtttt ccgtggtctt ttgcgtccgc cgcggaaatt gcctcctggt
     2341 ctggaactat tatttgaatt gtacattgtt tggttatcct cctgggatca gaatggaaga
     2401 tcgtcctcga tgatgtcgat cgagtcttcc ttgtcgtctt ttttggtgtt gttattgtcg
     2461 gaagcgtcat agttgtgtcc gttgccgctg tcctgggatt gtgatttgga atccaggaat
     2521 acgaccgaat cgcacactac ttcggtaaca tatcttctga cattgttttt ttcgtccatg
     2581 taatcgcggg tctgcaagcg gccgtccacg gcgaccatgc tgcccttgtt tacatatttg
     2641 ctaatgtttt cggcctgttt ccgccacgct gtgcagctga taaaatcagc cgtattatca
     2701 ttttgctggt tcgaactcgc gaaggggcgg ttaacagcca aagtgaatga tacaaccgca
     2761 atattgtttt gcgtgtatct cagttctgga tctctaacca gtctgccgac tagaactact
     2821 ctgttcaaca tggcttttcc tccgtattac ttttcttcgc gtgaaatgat tatgtggcgc
     2881 aaaacgtctt ctttgatctt catgacgcga tcggcttcca tggtggctgc cggttcggca
     2941 ttcagcgaga gaacgacata atagcctttt ttgcaatcgt cgatctcgta ggccagctcg
     3001 cgcattcccc attcgttgac tgcgatatcg gcggctcctt gatcggtgag gattttgttc
     3061 atttcctcga tcaaggcttt tcttgcctcc gcttccactg tcggacggat gatgtacatg
     3121 atctcgtatt ttctcatttt tcttcctcct tttggtctgt tggctgcttt tgcagcaagg
     3181 atgtgtgaat tctcacggaa tgttattata tcataatcat tattatttgt ctactaaata
     3241 taataggcgg aaaacggttg ctcatcacac ttcgacattg tatatttttt tgaattcgtc
     3301 tttcatcctg agcggaatca ggaaaataga ggaaatgctt acgactgccg cgaacatgat
     3361 gatgatccgg tagtcgagtc ccgaatattc cgcgatcagt ccggcggcca cggcgccggc
     3421 aatcgaccca aaattccata tcagttgctg ggtggcgttc acccttcctc tgatattgtc
     3481 ggatacatat gtctgtgtgg ctgccatgcg gatattgaac gatgtcactc ccagaaggcc
     3541 tacgatgaaa ctcatggtga tcatcagaat gtaaggcatg aaaagcatgg tcgcatccaa
     3601 tacctcaacg gtaaaataga caacgatggc aatagtgaat tttttctcga ccggaagacg
     3661 gaaaacataa tggaccaacc cgcctacgat cctaccgatg gctccggcag atatcaggaa
     3721 cgagtagtcc tgaatcgtga aattgggact cgagacaaaa aacggcatgc gtaaaagatc
     3781 gccaacggcg tacacgaacg caaaggcccc gaacagcaag gcgataccga gaattcccct
     3841 ttcgtgtttg aaataatgga tgctttccct gatatcggta atgaactgcc gtttctcgat
     3901 ctggctgtcg ttaagatttt ccttgacgtt tatcagtagt tcgatcgatg ctgtcactac
     3961 gaatgtcacc gcgttgaaaa tcatgagaat ggcggtgccg tttgagaagt tgtcgataaa
     4021 ataggcggcg atcggcgcca tgatggctgc cgacaaaggc cagatcaaac tggcgatcga
     4081 ataagccttg gaatgcgatc ccttgggaac ggtttccgga aacaggctca tgaatgccgt
     4141 ttgatagatg gtatcgatcg ttccgatcaa ggcgcttatg gctgtgaata cccagacgtt
     4201 gaagaatccg gtaaacaata ccaggcccat cgccagaaaa aacaaagaat aaaaataatc
     4261 gatagtaaca ataatattcc ggcgtgaatg gcgatctacg tatggccctg ccaaaaagct
     4321 ggccacaatt ctcgggacga cgttggcgac gaaaaacatc gccagcatta aaggcgatcc
     4381 cgtttctgta tatacaagta ttccgaatgc gacaccggct gctgcacttc ccaaagcgga
     4441 aatgaacgag ccgattgtca caatcgagaa gtttcggttc caaaggcgtg tcatgtcaat
     4501 actccctcat caagcttgcg acatctgatg tttatttgtt gttaagacgg ttgatgacat
     4561 atgccattgc atatgccgtt gctttcttcc gaatttcatt acgatcaccg gaaaactgtt
     4621 ttctttcggt gcttttatct ttggttatta caaaaccgaa aaacaccgtt cccaccggta
     4681 tattctccgt gccaccgtcg ggcccggcta ttccggtcgt agcaatggca atgtttgctc
     4741 cggaaacatt ttgaagcctg tcggccattt ctgaagctac ttcttcgctt actactccgt
     4801 atttctggat tgtctttgga ttgattccca ataaatccat ttttgcctgg ttggaataaa
     4861 ccacaaaagc ttccttcatg attttcgaag cgcccggctg gtctaaaatc cttgaggcga
     4921 tcatgccgcc tgtcaccgat tccgcagtcg aaacctgcag gttccgtttt tccaatgttt
     4981 caaagaaggc ttcagccaat gtccggttgg gtggaaagaa cagatagtcg gaaaaaatct
     5041 ccacaaagcg ttttctggcc tttgtcaggt atttctgttc cgaagaggat aattcgaccg
     5101 taggttctcc ttcgtcgtca ttaatcctta tccccacgcg tttgtaattc agctcatgtt
     5161 cctttatcag attttcgatt tgttctctgt cgatgccaaa caaaagatac cgttcgctga
     5221 aacgaatgtt ttcctcgttt ttagtttcca tgttttatta cctcacaaat tgaaacggaa
     5281 gtggaacacg tctccgtctc tggctatgta atccttgcct tccagtctgt atttccccaa
     5341 ttcctttgct tttgtctcgc taccgcaggc aaccagatct tcataagaaa tcgtttctgc
     5401 ccggataaaa cccttttcaa aatcggaatg aaccactccg gcgcattccg gagctttcat
     5461 gccttttttg aatgtccaag cccggacttc cttctccccg gcggtgaaaa atgtttgtag
     5521 tcctagcaag tcgtaacttt tgcggatcaa atcagaaagt cccgactcct ccattccgaa
     5581 atcggataaa aaatctttct tgtcttcatc gctcagttcg gatagttctt gctcgatttt
     5641 agcgctgata actgttgtgg cagcgccttc cgaagtcgct ctttccacga ctttcctgac
     5701 ttgtggattt gcgccataat cagccatttc cgactccgag acattgcata cgtaaagcat
     5761 cggtttggcg gtcaaaaaac tgaagttttt gataatcttt ccttcttcat ccgttaggtt
     5821 tacagcccgg atcggtattg acgattctag ggcatccttc aatttcaaaa ggatggcata
     5881 ttcaatggct gtttcctgat cggctttcat ttctgctttt ttttgaatct ttggcaatct
     5941 tttttcaata acgtccagat cggcaaaaat aagttcgatc atgattgtct ccatatcccg
     6001 taaaggatcg atcgaacctt ccacatggac aacgttcgga tcatcgaaac atctgaccac
     6061 ctgacatatg gcatcgcatc cgcggatatg cgacaggaat tggtttccta gaccttcccc
     6121 tttggacgct ccgcgtacca gaccggcgac atcggtgaat tcgaatgtcg tatatattgt
     6181 tttccttgat gtatacattt tcgtaagaac gtcgaccctg tcatccggca cttccacgac
     6241 tccgacattc ggatcaatcg tcgcgaatgg atagttggcc gccagaactt tagatttggt
     6301 tatcgcattg aacagagtgg atttgccgac atttggcaat ccgattattc cggctgtcag
     6361 tcccattttc ctcatctccc gattatatta ttttattaca ttacattact ttatacaata
     6421 gtcgccaaaa caaaacggga aagggaaaat cgccttcccg tctgtcgggt ctatatcgtt
     6481 caacccgcga tggcaaacag tttctcggca ttcttcgatg ttatgagccc gatggtctct
     6541 gtcggcatat tccttatgcc ggcgatttct tcggcaaccc tgaccacgta agccggttcg
     6601 tttgtttttc cccggtacgg tgtcggagcc agataaggcg agtcggtttc aatcaataaa
     6661 tattcaagcg gaatgattgc ggcaacttcc ttagcggctt tcgcattttt gaaagtaact
     6721 ggcccaccaa gagatatgaa catgttcagt ccgatgaaat ccttggcgga ttccacactt
     6781 ccggaatagc aatgcatgac cccgccggcc gattgttttt tgtgcttcat tagaatattt
     6841 aaagtgtcgg ctgtggcatc gcgcatgtga atgatcatgg gaaggttgtt gtcgccggca
     6901 atcccaatct gacgaatgaa tgcgtctttc tgcttctcca tcagggattt gtcccaataa
     6961 taatcgagac cgatttcccc gattgccact actttttcgt gatgacaatg ttcttttagc
     7021 cattcgtaat cggcatcggt gaccgggccg atgttttccg gatgaaaacc gatggccgcg
     7081 aatactgccg gatacctttc cgccaattcg atcgcttttt tatttgattc gagatcatat
     7141 ccgggacaga tgatccttgt gacttttgcc agattggccc ttctgatgac ttcatcggct
     7201 tccggataaa gcagactgtc gtttagatgg gcgtgcgaat cggtaaacat ttatttgttt
     7261 ttcccgaacg ctttcaggca ttttgaaaca atgtcatcgg ctgtaaaacc aaaatgtttc
     7321 aaaacatcgg cggctttccc gctcgccccg aaagtgtcga ttccgacaac gttgccggca
     7381 aagcggtacc aaagtcccga agctcccatt tcaatcgcta gtcttttgtc aatcggaagg
     7441 gataatatct ggttttggta tgcctcgctt tgaccaagga aagtctccat cgaaggcatt
     7501 gacactactc tgaccggtat ttgatgttcc ttgtatagtt tctcctgagc ttccatagct
     7561 agtccgagtt cggatccgga agcaataatg attcctgtgt aatccttgat atcactggcg
     7621 atataggcgc cttttttaaa atctaagtaa tccgattgga ttgcgacggt tagatcctgc
     7681 ctggtcaata taatgacggt tggtctatcc atggtttcca tcgccaaatg catggcatag
     7741 gctgtttcgt tggcatccgc cggacgcaaa accaacaggt tcggagtcga acgaaaagcg
     7801 gcaagttgtt ccaccggttc atgggtcggt ccgtcttcac cgacggctat gctgtcatgg
     7861 gtgaagatga aaagcgaggg gatccccatg atggcaccta gtcggatcgc cggtttcata
     7921 tagtcggaaa acacgaagaa tccaccggaa aatccacgta gtccatgtaa cgtgagtccg
     7981 tttacaattg ctcccattgc gtgctcgcgt acgccgaaat tgatattcct tcccgtccgg
     8041 ttttctatgg agaaatctcc gtttatgcct ttgacttggg tcgaaggggt cagatcggcg
     8101 gaccctccga tcaaagcttg attgttttcc tgatacgttt tcaacaatct gccgatcgat
     8161 ttgcgggtcg cttctttttg accattttcc aacaatccga tgttttttac gaattcaagg
     8221 tcgcattcgc cattcattat ggaggttaat tgcttatatt cttccgggta ttttccggca
     8281 tattctgtaa tcatcgcatc ccaaactttt ttctctgttt gtccgcgttt gatgaatttg
     8341 tcggaaaaat ccgctgtgga agtatccaaa acacagaagt cctccaagga ataaccggct
     8401 tctaatttca tcctcaaggc atcttctact ccgatcggag atccgtgaac ggcacttgtt
     8461 ccttgcttgg cggaaccttc tccgatgatt ccttttattt ctatgaatga cggtttatct
     8521 gattgccgac ccttttcaat ggcaatcgac aaaccgatca catcgtcgga tgattcgact
     8581 ttctggtaaa aaaatcccat gctttccatt tttgctttga tgtcgtccga agtcgccttg
     8641 cttgtcggtc cgtctaactg aatgtcattt gaatcgaaca ggacggttag tttgtttaat
     8701 cgcaaatgtc ctgccagact caaggcttcc atagcaacgc cttcttgaag gtctccgtct
     8761 ccgcaaaggg tatatgtgtc atggttgacg acggggaatc cgggtctgtt gaaagtggcc
     8821 cccagatatg cttccgccaa agccattcct accgccatgc cgattccctg acccaatggc
     8881 ccggtagttg cttcaactcc cggagtgtga cggtattccg ggtgacctgg tgtcattgaa
     8941 cctaattgcc tgaatttttg gatctcccca aaaggaatgt cataaccggc aagaaaaagc
     9001 gtcgaataga gaagcgccga tccgtgtccg gccgacagaa tgaaacgatc gcggttgaac
     9061 cagtctggat ttttcggatc tccgtcgaga tgatattgaa acaacgaata catgatcgga
     9121 gcggcgtcaa gaacgatgcc gggatgtccg ctgtttgcct tatttatcat atcgatggct
     9181 aaaaatttca tattcaagat cgtatttttc atgatgatcc tccctgtcaa aaaaagatag
     9241 gattgatttt acgggaatat tttcgggaaa actgaattgt gtccgtattt gcgaccgggc
     9301 caaatataat tataccatac ttgcctgcgc cgagccattt ttaccgtgta tttcttttca
     9361 taagaacgta catgtttgaa atgcccgata actatgataa aataataagc ggaaagagga
     9421 gatatgcatg aatgtattga cacaaaggct atgcatccgc gatttcaagg aagacgactt
     9481 cgatgatctt tgggcaattt tcaaagataa agaaaccatg aaacatgtcc gaaattacac
     9541 caaaccggag accctcacct ttttccgttc tttctgtctt tccaaaaccc cgcctggagc
     9601 tttcgccgtt gttttgaaag acatagataa ggttattggg tatctgcttt tcaacaatat
     9661 tgatgaaaaa ggcatctacg agattggctg gatattcaac cgtatgtatt ggcgggaagg
     9721 ttacgcttat gaggccgcaa aagccatgat caaatacgca ttcgattcgc ttgacgcaca
     9781 caaaatcgtc gccgaagccg aagataccga caaatcggtc ccgctcatga aaaaactggg
     9841 aatgatccag gaagggattt tccgcaaaca ttgcctgacg gaaagcaaaa cgtggaagga
     9901 tctttactgg tacggaattc tcaaggatga tatttgtggt tgattataat atctgatatt
     9961 tttaatataa aaaagatccc cttacacagg agatcttttg ttttttaatc aattgctatt
    10021 cgatgatgtt gataaccgat ccggcaccaa ccgtatggcc gccttcgcgg atggagaact
    10081 tggttccctg ttccaaagcg atcgggtgaa tcaattcaac agtcatttcg cagttatctc
    10141 cgggcatgac catttcgacg ccttcggcca aattgatgac tccggtgatg tcggtggtat
    10201 ggaaatagaa ctgcggacgg tagttggtga agaacggagt atgacggccg ccttcatctt
    10261 tcgtcagaac gtagacctga gcggtaaatt tggtatgcgg tgtgactgac ttcggtttgg
    10321 caatgacttg tccgcgttgg acttcgtctc tgtcgacgcc tctcagaagc aagccgacat
    10381 tatcaccagc ctgggcgtag tcgagttgtt tgcggaacat ttcgactccg gtaacaactg
    10441 aagctttcgt cggtttgata ccgatgattt cgactggatc tccgactttg atcgttccac
    10501 ggtcgacacg gccagtaacg acggttccac ggccggtgat ggtgaagaca tcttcgatcg
    10561 gcataaggaa cggcttgttg atgtcacgaa ccggaagcgg aatgtattca tcaacgacat
    10621 ccatcagatg aagaagagtc tgttgttctt tttcgtcgcc ttccaaagct ttgagagcgg
    10681 aaccgacgac aaacggtgtt tcttcgccgg ggaatccgta ttcatcaagc aattcgcgga
    10741 tttccatttc gacgagttcg atcaattcgg gatcgtcgac catatcggct ttgttcaggt
    10801 agacaaccat cttgggaacg ccaacctgtc cggcgagcag aatatgctca cgggtctggg
    10861 gcatggcgcc gtcggcggct gatacaacca ggattgctcc gtccatctgg gcggcgccgg
    10921 tgatcatgtt tttgacatag tcagcatgtc cagggcagtc aacatgggcg taatggcgat
    10981 ttttggtctc atattcgata tgagcggtat tgatggtgat tcctctggcc ttctcttccg
    11041 gagccgagtc gatggacgca tagtccttga cttgggctaa accctgtttg ctgagcacga
    11101 gggtgatggc agctgtcaaa gtggttttac catggtcgac atgaccgatg gtaccaatat
    11161 taagatgggg ttttgtacgt tcaaattttt gttttgccat ttcaatattc tccttttatt
    11221 ctaaattttc tttatcgctt ttcagtcgat aatattttat aacaatattc aaaataatgc
    11281 aatactatta tgttttagcg gtttcgtata ttatccgttg cgtttggcaa tgatttcttc
    11341 cgctattgat ttcgggcatt cctcgtaatg tgaaaattgc atcgtatgtg ttgctcttcc
    11401 ttgggtattc gaacgaaggg ctgtcgcata tccgaacatt tgggatagtg gcaccttcgc
    11461 cgaaaccttc tgggctttgc cctgggtttc ctgagcttcg attcttccgc gccttgatgt
    11521 cagatctccg atgacgtttc cgatatattc atccggaatg acgacatcga cattcatgat
    11581 cggttccagt aaagtcggtt tgcatttttc cctggtctgt ttcaaagcta gggaagcggc
    11641 gatcttgaat gccagttcgg aagagtctac ttcgtgatat gatccgtcaa atagagtggc
    11701 tttgatgtct agaacgggat acccggcgac aatgccgcca agcaaggctt cctgcaatcc
    11761 tttgtctacc acgggaatgt attcccttgg aacgacgccg ccaacgattt tatcgacgaa
    11821 ttcataaccc ttgccgggat tcggttcgaa cttgatccat acgtggccgt actgaccgtg
    11881 tccaccagtt tgcctgatga atcgaccttc gcaatctccg gcctgtttga tggtttcccg
    11941 ataagagacc tggggttggc ctacggttgc ttcgacattg aattctcttc tcatccggtc
    12001 aacgataatc tccaagtgca gttcacccat tccggaaatg atcgtttgcc cggtttcctt
    12061 gtcggtatat gtacgaaatg tcggatcttc ctccgccagt ttcgacaacg caacgcccat
    12121 cttatcctgg tcggcccttg ttttgggttc gattgcaacg gagatgacgg gttcggggaa
    12181 tttcattgat tcaagcacca catcgatttt atcggaacac aatgtatcac cggtcgtcgt
    12241 atccttgaat ccgattgcgg cggcaatgtc gccggaatag acagcatcga tctctgtccg
    12301 atgattggca tgcatctgca atatgcggcc gagtctttct tttttacctt ttgaagtatt
    12361 gatgatatat gatccttttt ccgccacgcc cgaatagacc cggaaaaagg tcaatctgcc
    12421 gacgaacgga tcggtcatta ctttgaaagc caatgctacg aattccttgt tgtcgtcatg
    12481 tccgatgaca acttccttgc cttgtttgtt aaagccccgg atttgcggaa cttccactgg
    12541 cgacggaaga tagtcgataa cggtatctaa caacaatttt acccctttgt ttttaaaagc
    12601 ggatccgcag atgaccggaa aaaattcccg tgtcaatgtc gctttgcgga tggcttgttt
    12661 cattagggca acagatattt ccccgccatc caagtatgtt agcatcaact cttcgtcgaa
    12721 atcagctacc gcttcgatca acgttgtacg tttttgctcg acaattttca gtagctgctt
    12781 cggaatcggg atttcgacat agttttcttc gggttctccg tcgaaatgga atgctttcat
    12841 ttggaccagg tcaatgatgc cgtcgaatgt ctcttcggcg ccgatcggcc attggatggc
    12901 ttccgccttg gctccaagac ggttatggat ggattcgacc gaatgcgcaa aatctgcacc
    12961 tgctttatcc atcttgttgg caaacaccac ccggggaaca tgatactcat tggcttgtct
    13021 ccaaacggtt tcggtttgcg gctctacgcc ggcctgggcg tcgagcacgg ttacagcacc
    13081 gtccagcacg cgcagggaac gcgaaacctc gacggtgaag tcgacgtggc ccggggtgtc
    13141 aatgatgttc actcggtaat ttttccagaa agctgtcgtc gctgcggcag taatggtgat
    13201 gccacgttct tgttcctgtt ccatccagtc catttgcgaa gcaccatcat gtgtttcacc
    13261 cattttatgg atttttcctg tgtggtagag aacgcgttct gtagtcgttg tcttcccggc
    13321 atcgatgtgg gccatgatgc cgatattgcg tgttctctcc aagctgaatt cacgtgccat
    13381 aagaatttcc tttcagtgat taaaaacgat aatgtgcgaa agccttgttg gcttctgcca
    13441 tcttgtgcat atcttctttt ttcttaaccg atgcaccggt tccgttggaa gcgtcgataa
    13501 tttctttcgc taatctttct tccattgtct tttcgttgcg aaggcgggaa taaagtacca
    13561 gccatctgag gcccaaagtg acacggcggt catttctgac ttctcccgga acctgatagt
    13621 tctgtccgcc gatacggcgg cttctgacct ccagaacggg catgatattg ttcaaagctt
    13681 cgttgaacac ttctatcggt tctttgccgg tcgttgtctt tatgttttcg aaggctctgt
    13741 ataaaatgtt ctgtgcgaca ttctttttgc cgtcgagcat gattccgttt atgagtttgg
    13801 taaccaattt ggagttataa atcggatccg gtaatacatc tctttttact acttttcctt
    13861 tacgaggcat tgttggttcc tcctttcgaa gatgataagt taaatgttaa ttagttgtta
    13921 tttttttgcc ggagccggtc ttttcgctcc atatttggaa cgactttgct ttctatcttg
    13981 aacagcagtc gtatccaacg ttccacgaat aatgtggtaa cggacaccag gtagatcctt
    14041 tacacggcct ccacgtacga gaaccacact gtgttcctgt aatttatggc cgatgccagg
    14101 aatgtacgcg ttaacttcgc cgccgtttga caaacggaca cgagcgtatt tgcgcaaagc
    14161 cgaattcggc tttttcggtg tcatcgtggc aacacggaca cagacgcctc ttttttgcgg
    14221 actgttgaga tcggtatagc gtttctgcaa agtgttgaat ccaatattca attgcggagc
    14281 tttggatttc ttgactttgt catgacgtcc ctgtttgatc aattgattga ttgtcgacat
    14341 aatgttttcc tcctttcatg cccacacacc caggaggtgc gcttgtttct tttttattaa
    14401 ggttgcgttg gcaaccttca aatgtaatac attttttcac agcgatatta ttatacatta
    14461 acaaggcttt cctgtcaaca aaaaaatccg cgatttgaca cggattttca ttctttttcc
    14521 tgctcttctt ttttttcttt ttcttcaagc tcatatgctt tggaaagcag aattgcttta
    14581 tcgcgaggac gcaatctttt catgatccgg actacctcca gttccatgtt cggagaaacc
    14641 tcgtctccat aaggaatgac tcccgtttcg cttaccgcca ggccgatatc ggttccggaa
    14701 atcagataat ctgcggtcac tcccaagtaa tccgctatca acatcaccgt atccagtttc
    14761 atgttctctg acttgcgttg ataaagactc atcaaagtat gataagaaat gcctgttgca
    14821 gcgcaaaggt cttttctggt tttgttcttt tttttaagca acaaagtaat ccggtcgtaa
    14881 atggtttcca tattttcact cccaaatact attttatcat ttatcacaat ggttttatct
    14941 taaaaaagca ggcaaacgca taaatgaata ttgacaaaac atgcttttgc gcttattata
    15001 tgattatata tatgcgtttg cattataacg gtgatagaaa tggtatatgc caatttgaaa
    15061 gcggaaatgg tcaggaaaga catatctgcc acgcaaatgg ccggactttt gaatatcagt
    15121 ttgcggttgc tggatgaacg aatgcgttgc gaaagcgggt tcaccatcag ggaagtcaag
    15181 atcatcatgg atttgtttcc ggaatgcagc atcatggatt tgttcacaaa aaccccttgt
    15241 tgatatttat gggatcctct ctcagaaaca aacccgtccc ataggtatgc tcatgtgatt
    15301 gccaattggc aatcacatga tttttttcct tataaaatat aaaaaccgca tttttgtgag
    15361 gtatcacatt ttgcggtttt gtttttcaaa caagaaagcg gtctggcagc gacctactct
    15421 cgcacgcgaa cgtactaccc tc
//
LOCUS       DAOPJL010000025        14907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_4217_length_14907_cov_6.557635, whole genome shotgun sequence.
ACCESSION   DAOPJL010000025 DAOPJL010000000
VERSION     DAOPJL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14907
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4217_length_14907_cov_6.557635"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLP02_04280"
     CDS             complement(<1..521)
                     /locus_tag="PLP02_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF42693.1"
                     /translation="MNKFMVLAKGEIKRLFRYKIIVFSLIVSVIWALIIALTDLDFAV
                     SFVPFLVLLDAGMMAILYLGASYYLEKQEGSMKSVFVSPVRLSSVLAAKIVAALFMAL
                     LSLLIVLATAYAFHSDFHAKVLLMILYTVLVVVSHTAIGYLIILTSKDFMQMLLKYMW
                     VFLAFMVPMLLVV"
     gene            complement(514..1371)
                     /locus_tag="PLP02_04285"
     CDS             complement(514..1371)
                     /locus_tag="PLP02_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42694.1"
                     /translation="MYEVKDLVFSYPKGKKKAVDGISLSVEEGTIFGLLGPSGAGKST
                     TQKILCKLLSGYSGSIIFRGKDLKEYDKSFYENVGVGFELPVHFNKLTALENLKFFAS
                     LYKKNADYEKLLTKLGLYDARNQMVGEFSKGMKIRLNLIRALVNDPEVLFLDEPTAGL
                     DPANAKIVKDIILDCRKQGKTVFVTTHLMGDVEQLCDHVVFMNEGGITETSTVHDLKM
                     KYGKREIIVGYYEGKSLKNRTFPMQGIGNNVDFANLIKEKEIDTIHSGETTLEDIFIK
                     VTGGSINHE"
     gene            complement(1382..1984)
                     /locus_tag="PLP02_04290"
     CDS             complement(1382..1984)
                     /locus_tag="PLP02_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF42695.1"
                     /translation="MPLETAEETKEKIMEEALHEFGSKGYDKASTNLIHSRAKVSKGL
                     IFKIFKTKSDLYYLVFKKAIDDMIQCVGELDYSDAEDVFAKIVKVTAWKLDYANKNKD
                     AVAVMMEAITKPPKGLEEKIARHYKDLTVLSINQFFIDIPMEKIRDEYTKEDVIRNIM
                     IGVAGLTETYVKGDLSVEYMQTIKDESLGFLKILMRGMEK"
     gene            complement(2119..>2739)
                     /locus_tag="PLP02_04295"
     CDS             complement(2119..>2739)
                     /locus_tag="PLP02_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF42696.1"
                     /translation="NYSHTLVNALVYHIDNLPVKDDRLRPGIVHRIDKDTSGLLMVAK
                     TEKAQILLQDELKRKHTKREYIALLDGVILNETGTIDAPIGRDTKDRKKMAVVKDGKP
                     AITHFEVLERFFDKTLVSCRLETGRTHQIRVHMAYIGFPLVGDYVYNPKVKKNDFGQY
                     LHAKTIGFTHPKTGKWMEFSTELPDTFQEELDKLRKEDTFASAFPA"
     assembly_gap    2740..2839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2840..3400)
                     /locus_tag="PLP02_04300"
     CDS             complement(2840..3400)
                     /locus_tag="PLP02_04300"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPF42697.1"
                     /translation="MKKIWPWLLGILLLVIVDQVSKYLIVINFAQLGDKIDIIGDFLQ
                     LKYVRNAGAAFGLGSDCICVLTDTMYSSNPLDFSNVGFSTYFFIAMFLVALVAFGWLF
                     AKNDFGDKRRIWYSISLMLLIAGALGNTIDRIFQPDHCVVDFIAFSTIWDAVFNVADA
                     CLTVGITLLLIDQFLLEPRRSKQNAS"
     gene            complement(3403..3774)
                     /locus_tag="PLP02_04305"
     CDS             complement(3403..3774)
                     /locus_tag="PLP02_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42698.1"
                     /translation="MMQRYFSELKKLVSEYKPGRIADAIFAGFLFSTIIFVPVYLILT
                     ELMIVYVYLYYEITALFILAALGHMAIWHLLTKQSLARKIPNSGCDLNFLFFSSMLFF
                     GLVIIVIGLLIIFVMLPIWMV"
     gene            complement(3776..>4549)
                     /locus_tag="PLP02_04310"
     CDS             complement(3776..>4549)
                     /locus_tag="PLP02_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42699.1"
                     /translation="TYALPTSYENYTNEEINTISYYDKDISVSTSIIVAYEAMASVDP
                     SIVINYTSKIIVFGKSSYLSHYDEIAFGDEFVQVVGDDSLVITDSSLIKENTTAGNTY
                     DFTFINADGDTYTVGLTKDLDTGLFGITLYPYSLVDPETSYPVFAINDSSIGGSSGGL
                     LQTLAVYNMLSSEDITHGLKVAGTGTMSIDGTVGNIGAVKQKVLTAYLNHVDVFFMSP
                     DDYSTAVNVCEAYNIDYSSWLVSVATFQDALDYLEGLGE"
     assembly_gap    4550..4649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4685..5158)
                     /locus_tag="PLP02_04315"
     CDS             complement(4685..5158)
                     /locus_tag="PLP02_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPF42700.1"
                     /translation="MKREDYISWDEYFMGVAMLSGLRSKDDSSQVGACIVNQKNRIVG
                     IGYNGFPIGCEDDCFPWEREGDFLDTKYPYVVHAEANAILNTSVDLSGCRIYVTLFPC
                     NECAKLIIQAGIREVVFLEEKNTGTDMDKAAKRLFAAANVKLSHLENFNLKVVRQ"
     gene            complement(5155..6069)
                     /locus_tag="PLP02_04320"
     CDS             complement(5155..6069)
                     /locus_tag="PLP02_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPF42701.1"
                     /translation="MRINPEVRNAIRRNEPVVALESTIISHGMPYPANIETAIACENK
                     IRERGAVPATIAIIEGEIVVGLSLSEIERIAAPDANVMKVSRRDLAYATYKHMDGALT
                     VSGTMIAAKMAGIRFFATGGIGGVHRNACSTMDISADLDELARTQVYVVSAGAKAILD
                     LPKTKEYLETKGVPVIGYQTDELPAFYTRESGVGVDFRFDSPEEIAGYLLEKEKQGTG
                     GGVLIGNPIHEQFAMSKKEIDEAIDKALAEAAEKGITGKEVTPFLLSRIVFHTKGKSL
                     ESNKHLVYSNAELAAKIAVSFHGLEKKK"
     gene            complement(6026..6574)
                     /gene="msrA"
                     /locus_tag="PLP02_04325"
     CDS             complement(6026..6574)
                     /gene="msrA"
                     /locus_tag="PLP02_04325"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPF42702.1"
                     /translation="MKTIIFAGGCFWGVEAYFKRLNGVMETTVGYIDGYTENPTYKEV
                     CDGSGHAEAVRIAYDEAKISLETLLDHFFRIVNPTEKDRQGPDIGRQYRNGIFLLSPY
                     DMEKSQNYVNIIKGKYIDPIQTIVKMAPIFYEAELYHQDYLDKVPNGYCHINLNLASN
                     SEMKEEYRHEDQSRGQKRHQKK"
     gene            complement(6571..7272)
                     /locus_tag="PLP02_04330"
     CDS             complement(6571..7272)
                     /locus_tag="PLP02_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPF42703.1"
                     /translation="MPSFERLRLLIGDEGMNKLANAFVMVYGIGGVGSYVAEALARSN
                     IGHIALIDSDLIEESNINRQIFATIKTVGSPKVEIAEKRLHDINPALKIDIYFQRATK
                     DNIANFLALNPMWVIDAIDDVETKASLLENCRKKRINVISSMGFANKFHPEMIEIAML
                     SETSVCPLARTMRQKLGRFGICDQIPVVYSREKPTATTQAKPLGSTAFVPAAAGLIIA
                     SYVVNKIIGRDDVKS"
     gene            complement(7313..7747)
                     /locus_tag="PLP02_04335"
     CDS             complement(7313..7747)
                     /locus_tag="PLP02_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42704.1"
                     /translation="MRSGKKVRIYSEFAIRMIVFYATMILLLPIILVVTYLFPIGGSF
                     NNLVFMFWAAIAVLCLTGIGTGILFLKRDHLKRRVRPSYRNEFIYLMMISAFGLLGFA
                     VMYDYLGFDRQYIANFLIVILALMLYLLIMLGRKYFKFNLYK"
     gene            complement(7744..8874)
                     /locus_tag="PLP02_04340"
     CDS             complement(7744..8874)
                     /locus_tag="PLP02_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPF42705.1"
                     /translation="MKKKPISDELLNRIIKYLAIALLTLAVIFMATLFSDVWNWIFKA
                     IKTVVVPVVFAYFLALIMHPLLKYLEKKGVGPRWLSLAIVFLLMIAIIAAFFYFIIPA
                     VVREITAFFNDDFQKIIGYLTSAELRDNFILGSDIYDKIFNYVAGTDILNTWLDSLIP
                     TFLAGMTDTLIPILTAVAIIPIMLLYYLLDYEMIGDRLRSIIPQKHEKRVADLGSRLN
                     QTVGAYLRGQLILMAAIGAVATIVYKLIGLKYYFAFGLLVGITNIIPYFGSIIAAIPP
                     IAYAAISGVAPGPLIVLAVNIGLQFVEGNIFQPIIMAKQLEMHPLVIIISILFFGALF
                     GTFGVVFASPIAAAIRVFYNFFREQKQLNELSPEGGGGKTKT"
     gene            complement(8947..10263)
                     /gene="hisS"
                     /locus_tag="PLP02_04345"
     CDS             complement(8947..10263)
                     /gene="hisS"
                     /locus_tag="PLP02_04345"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPF42706.1"
                     /translation="MITKIKGTHDILPDEAKKWEDLEHVIRQVSRIYNFQEIRVPIFE
                     ATELFHRGIGDSTDIVKKETFDFADRGSRMITLRPEGTASIVRAVIENKLYATMPQPL
                     KLFYYGPMFRYERPQKGRQRQFHQFGAEAIGSESPYVDSEIIAYSVTFLKALQIPGIV
                     VRINTLGDASSKENYKQVLKEYLTPQIGGLCEDCQRRYLENPLRVLDCKVDSDSTILK
                     NAPKPKDSLSEKAEAHFRQVLANLDRMGIDYVVDPSLVRGLDYYTHTVFEIDSNLETL
                     GSQKTLCGGGRYDKLSESLDGPSLPSVGFAFGLERLLLALDALEEQEIPDSIHAFMVS
                     LGEEAREKSLPIIEMLRHGGLVVEHDYDGKTLKAQFKQAEKFNPMFYLFYGSDEDKKG
                     VVNVKNAKTGVQEEIPFNKLYAELVTLIRKEHESCSGNCASCGGNC"
     gene            complement(10348..10608)
                     /gene="acpP"
                     /locus_tag="PLP02_04350"
     CDS             complement(10348..10608)
                     /gene="acpP"
                     /locus_tag="PLP02_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPF42707.1"
                     /translation="MDKKQVVFEKIKEIIIQELGVKPEKVVMDAGLADDFGADSLDAL
                     ELFNQIESEFLITISDEAAMKMKDVSDLVEYVVEHVSDKYFG"
     gene            complement(10700..11140)
                     /gene="dtd"
                     /locus_tag="PLP02_04355"
     CDS             complement(10700..11140)
                     /gene="dtd"
                     /locus_tag="PLP02_04355"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPF42708.1"
                     /translation="MKVVVQRVAKAGVSVEGKNISSIGKGYLLLVGIGSNDTETEIEY
                     VAGKIAKLRIFPDDNDKMNKSVIDISGDILAVSQFTLYGDISDGNRPSFTKAMEPVKA
                     QAYFDQFVSRLSALIGKEVKKGVFGAHMEVELTNDGPVTIIYER"
     gene            complement(11142..13334)
                     /locus_tag="PLP02_04360"
     CDS             complement(11142..13334)
                     /locus_tag="PLP02_04360"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPF42709.1"
                     /translation="MDREQLYLPLAEAISPYLSKPEHLDFIRGAFDYAYDKHNGQYRK
                     SGDPYITHPVQVAVTLAEYRVDPNTITAGLLHDILEDTSGTYEELKSKFGDEVADIVD
                     GVTKLHLLQYEGTSNKVTEQAKNHQKMVLAMARDIRVIFVKLSDRLNNMRTLSYLDHE
                     RQIRISRETLDIYAPIAHRLGIYRLKAELEDLAFKYLYPNEYLEIARLIKDKKGTREA
                     DIKTMEVELEELLKSEDFHFEISGRIKNIQSVYRKMQLKHLDFEEISDLLALRVIVDT
                     VSDCYRAIGIIHSKFIPVPGKFKDYIAMPKPNMYQSLHTTVVNKGKIYEIQIRTREMD
                     KVAEKGVAAHWAYKESQSGSHSRKNIEDIVSSKLRWYSELIKYTEESASDEEILDIFT
                     DDILNANVYVFTPKGDIIDLPAGATPIDFAFRIHTKIGEKMVGAIVNGKIQSLDYTLK
                     TGDMVEVKTSQNAVGPNNNWLKMAKSQNARSKIKNFLNRQKREYLVETGKQEFEQELQ
                     SRKVQIDITDKLCRDLFGNKGVKSEEDLYYEIGKNVISPVGAVNLMTGKETISEEELI
                     EKINTPDERRQQKTRTLHNDINIIVEGLTNPSVRLANCCNPILGDNIVGYVSKNSGII
                     VHRDVCNNLDYYDKARLIEVYWGSNVTKRYETSLKIIVQNRDNILAEIINVATSSKAK
                     INQVSAQTNKASEGIIRMKLEIGNVVELDQVVANIQKIRGIFSIERIC"
     gene            complement(13363..13875)
                     /locus_tag="PLP02_04365"
     CDS             complement(13363..13875)
                     /locus_tag="PLP02_04365"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005968670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPF42710.1"
                     /translation="MKFEDYIKNVPDFPIPGIQFKDITPLIGSGEAFHAAVSELAEFG
                     KNLEATKVIGPDARGFIIGAPVACQLQVGFVPVRKPGKLPREAISYDYELEYGKNTLC
                     MHKDAIQPGEKVLIVDDLLATGGTMGATIKLVEQAGGVVVGLGFLIELVDLGGRGKCQ
                     GYPIKALIKY"
     gene            complement(13865..>14907)
                     /locus_tag="PLP02_04370"
     CDS             complement(13865..>14907)
                     /locus_tag="PLP02_04370"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease
                     C-terminal domain-containing protein"
                     /protein_id="HPF42711.1"
                     /translation="KSAIVFTIDESGKAKGSARAFGDDNILKMLQVNEDVILKYGGHQ
                     QAAGLQINPADLEILRQRLNQISTHQETPPLKVDMAINLAEVSLETVRKLQDFSFFTA
                     TFLFTGLYVRKKQLIRDQHTKLQVESDGKLFDLLAFNNPDYFYSLETGDRINVVGGIG
                     INTWLQKTVIQITAKDIECKDFQTLDFRHVSFWRDQLAKVLQKDAVLISDRTIAGGLK
                     VQKDGTVIILPRAFSQPLKAVLNKDYLGRCYRIMIREQSETLDHFARFFGLDTEETAI
                     ILDIFRDLGFVEVEGNHYIPVDAPPKKDLYQSAKYNHWRVKADLIEKTYAASQRDSKN
                     IIITHLEAYNEI"
BASE COUNT         3621 a         3468 c         3408 g         4210 t
ORIGIN      
        1 atcaccacca aaagcatggg gaccatgaac gccagaaaaa cccacatgta tttcaaaagc
       61 atctgcatga agtccttcga tgtcaggatg atcagataac cgatcgccgt atgcgacacg
      121 acgaccaata cagtatagag tatcatcagg aggactttgg cgtgaaaatc gctatggaag
      181 gcataggcgg tcgcaagaac gatcaaaagc gacaacaacg ccatgaacaa ggcggcgacg
      241 atcttggccg caagaactga cgaaagtctc acgggcgata cgaaaaccga tttcatcgat
      301 ccctcctgct tttcaagata gtaggaggcg ccaagataaa gtatcgccat catgccggca
      361 tctagcaaaa cgagaaacgg tacgaatgac actgcaaaat ccaaatcagt caaagcaatg
      421 atcaaagccc agatgacgga aacgatcagg ctgaacacga tgatcttgta acggaaaagt
      481 cttttgatct cgcctttggc gagcaccatg aacttattca tggttgatgc ttcctccggt
      541 cactttgatg aaaatgtctt ccagagtcgt ttcgccactg tggatcgtat cgatttcttt
      601 ttctttgatc agattggcaa aatcgacatt gtttccgatg ccctgcatcg ggaaagtccg
      661 gtttttcagg cttttacctt cataatatcc gactatgatt tcccgttttc cgtatttcat
      721 cttgagatcg tggactgtcg atgtttcggt gatccctccc tcgttcatga aaaccacatg
      781 atcgcaaagt tgctcgacat cacccatcag atgcgttgtc acaaacactg tctttccctg
      841 cttccggcaa tctaaaatga tgtccttgac aatctttgcg ttggccggat cgagaccggc
      901 tgtcggttca tccaaaaaca gcacttcggg gtcgttgaca agcgctctga tcaggttcag
      961 cctgattttc attcctttgg agaattctcc aaccatctgg tttctggcat catatagacc
     1021 gagtttcgtc aacagttttt cgtaatctgc gtttttcttg taaagcgaag cgaagaattt
     1081 caggttttcg agtgccgtaa gtttgttaaa atgaacgggt agttcgaacc cgacaccgac
     1141 attttcataa aatgatttgt cgtattcttt caaatccttt ccccggaata taatggatcc
     1201 cgaataaccg gaaagcaatt tgcaaagaat tttctgtgtt gtcgacttac cggctcccga
     1261 aggccccaat agtccgaata tcgttccttc ttccacggat aatgagattc cgtccacggc
     1321 ttttttcttg ccttttggat aggaaaatac caaatctttc acttcataaa tcgctgccat
     1381 gtcacttttc cattcctctc atgagtattt tgagaaaacc tagactttca tcttttatgg
     1441 tttgcatata ttcgactgac aaatcacctt tgacataggt ttccgtcagg cctgcgactc
     1501 cgatcatgat attccttatg acatcttctt tcgtgtattc atcgcggatt ttttccatcg
     1561 gaatatctat gaaaaattga ttgatcgaca aaaccgtcaa atccttgtag tgcctggcga
     1621 tcttttcctc caggccttta ggcggtttgg tgatggcttc catcatgacg gcgaccgcat
     1681 ccttgttctt gttggcgtaa tcgagtttcc acgccgtgac tttgacgatt ttggcaaaga
     1741 catcttccgc atcagaataa tccagttccc cgacgcactg tatcatatca tcgatcgctt
     1801 ttttgaacac cagataatat agatcggatt ttgtcttgaa tatcttgaat atcagtcctt
     1861 ttgacacttt agccctggag tggatcaggt ttgtggaagc tttgtcataa cctttggaac
     1921 cgaattcatg caaggcttct tccatgatct tttcttttgt ctcttcagcc gtttccaaag
     1981 gcatcgcccc ctcgtgacca cgtggtcaca cgtatatgat actcaattta ggatttcttg
     2041 tcaagacatg attgactttt cgcaacaagt cgttcttttg attctatata caaaaaaggc
     2101 ggtgccaaga ccgccttttt atgcaggaaa cgctgaagcg aatgtatctt ctttcctcaa
     2161 tttgtccagt tcttcctgaa atgtatcggg aagttcggta gagaattcca tccattttcc
     2221 cgtctttgga tgggtgaacc cgattgtttt tgcatggaga tactgcccga aatcgttttt
     2281 cttgaccttt ggattgtata catagtctcc cactaacgga aatccgatat aagccatgtg
     2341 gacgcgtatc tggtgtgtcc tgccggtttc gaggcggcaa gacaccagag tcttgtcgaa
     2401 aaagcgttct aagacctcaa aatgtgttat tgccggtttt ccgtccttga caaccgccat
     2461 cttcttcctg tcttttgtat cgcgtccgat cggcgcatcg atcgttccgg tttcattaag
     2521 gatgacgccg tccagcaaag cgatgtactc tctttttgta tgttttcttt tcaactcgtc
     2581 ttgcaaaagg atctgggctt tttcggtttt ggcaaccatt aataggccgc tagtatcctt
     2641 gtcgatccgg tggacgatgc ccggacgcaa tctgtcgtcc ttgacaggaa gattgtcgat
     2701 atggtaaaca agggcattca ccagggtgtg gctgtagttn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnt cagctggcat tttgcttgct ccgtcgtggc tccaagagaa
     2881 actgatcaat caaaagcaag gtgataccaa ccgtgaggca ggcgtcagcg acattgaaaa
     2941 ccgcatccca aatcgtcgaa aaggcaatga aatcgaccac gcagtgatcc ggttggaata
     3001 tccggtcgat cgtgtttccc aatgctcccg caatcagaag catgaggctg atcgaatacc
     3061 aaattcgcct tttgtcgccg aaatcgtttt tggcgaacaa ccatccgaaa gcaactaacg
     3121 cgaccagaaa catggcgata aagaaataag ttgaaaatcc gacattggaa aaatccaaag
     3181 gattcgagga atacatggtg tctgtcaaaa cacaaatgca atcggatccg agtccgaaag
     3241 cggcgccggc attcctgaca tattttagtt gcaggaaatc gccgatgatg tcgatcttgt
     3301 cgccgagttg ggcgaaattg atgacaatca gatatttcga tacctgatct acaatcacca
     3361 aaagcagaat tcccaaaagc caaggccata tttttttcat agtcacacca tccatatcgg
     3421 tagcattacg aagattatca gaaggccgat gactattatc acaagcccga aaaacaacat
     3481 actggaaaaa aacaggaaat tgagatcgca tccgctattc ggtatctttc tggcaagcga
     3541 ttgcttcgtc aaaagatgcc aaatagccat gtggcccaaa gcagccagta taaaaagtgc
     3601 ggttatctcg taatagagat agacatagac gatcatgagt tcggttagaa tcagataaac
     3661 cggaacgaaa atgatggttg aaaacaaaaa tccggcgaat atggcatcgg caatccgacc
     3721 cggtttgtat tccgatacaa gcttcttcag ttcgctaaag tatctttgca tcatgtcatt
     3781 cacccaggcc ttccagataa tcgagggcat cctggaatgt ggctacggaa accaaccacg
     3841 atgaataatc gatgttatag gcttcacaga cattgacggc ggtggaataa tcatccggag
     3901 acatgaagaa cacgtcgaca tgattcagat aagcggtcaa cactttctgt ttgactgcgc
     3961 cgatatttcc gacggtgccg tcaatcgaca tcgtgccggt accggcaact ttcaatccgt
     4021 gagtgatatc ctcgcttgaa agcatattgt agacggcaag cgtctgaagc agtccgcccg
     4081 acgaaccgcc gatcgacgaa tcattgatcg cgaagacggg ataactggtt tccggatcga
     4141 ccaggctata aggataaagc gtgattccga agaggccggt gtcgagatcc tttgtaagtc
     4201 cgacggtata tgtgtccccg tcggcattga tgaaagtgaa atcatatgtg ttcccggccg
     4261 ttgtgttttc cttgataagc gaagaatcgg tgatgacgag tgaatcatcc ccaacaacct
     4321 ggacaaattc atcgccaaaa gcgatttcgt cataatggga aagataggac gacttcccga
     4381 aaacaatgat tttgctagta tagtttatca cgatggatgg atcaactgac gccatcgctt
     4441 cgtatgcgac gatgatggat gtcgataccg agatatcctt gtcgtaataa ctgatcgtgt
     4501 ttatttcctc gttggtgtag ttttcataac ttgtcggtaa ggcgtatgtn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttgatatgca acgaatattt ttcgagcaat
     4681 tcgttcattg tctcacgact ttcagattga aattttccag atggcttaat ttgacattgg
     4741 cagccgcaaa caatcgtttg gccgctttgt ccatatcggt tcctgtattt ttctcttcca
     4801 gaaaaaccac ttcgcggatt ccggcctgga taatcagttt ggcgcactcg ttgcagggaa
     4861 aaagcgtcac gtaaatccga catccggaaa gatccacgct ggtgttcaga atcgcattgg
     4921 cttcggcatg gacgacatag gggtatttcg tatccagaaa gtcgccttcc cgttcccagg
     4981 gaaagcaatc gtcttcgcaa ccgatcggga atccgttgta accgattccg acgatccggt
     5041 tcttctgatt gacaatgcag gctcccacct gactggaatc atctttgctt ctgagaccgg
     5101 aaagcatggc tactcccatg aaatactcgt cccaggatat gtagtcttct cttttcattt
     5161 tttcttctcc aatccatgaa atgaaacggc gattttggca gccagttcgg cattcgaata
     5221 aacaaggtgt ttgtttgatt caaggctttt ccctttggtg tgaaagacga tccgggaaag
     5281 taaaaaaggc gttacttcct tgccggtaat acctttctcg gccgcttcgg ccaaggcttt
     5341 gtcgattgct tcgtcgattt cctttttcga catggcgaac tgttcgtgaa tcggatttcc
     5401 gatcaggacg ccgccgcccg taccttgttt ttccttttcc aaaagataac cggcaatttc
     5461 ttccgggctg tcgaatctga agtctacgcc gaccccggat tccctggtgt agaaagccgg
     5521 aagttcgtct gtctgataac cgatgacggg aacccctttg gtttccagat attccttggt
     5581 cttcggcaaa tccagtatgg ccttcgcacc ggcggaaacc acatatacct gagttctcgc
     5641 tagttcgtca agatcagccg aaatatccat ggtggagcag gcgttccgat gaacgccgcc
     5701 gattccgccg gtcgcaaaaa atcggattcc ggccattttg gccgcaatca tcgttccgga
     5761 aacggtcagg gctccgtcca tgtgcttgta agtcgcataa gccagatccc ttcgagacac
     5821 tttcatgaca ttggcatcgg gagcggcaat cctttcaatt tcgcttaggg aaagaccgac
     5881 cacgatttcg ccttcgatta tggcaatcgt ggcgggaacg gctcctcttt cacgaatctt
     5941 attctcgcag gcgatggctg tctcgatatt ggctggataa ggcattccgt gggaaatgat
     6001 cgtcgattcc aaagcgacca ccggttcatt tcttctgatg gcgtttctga cctcgggatt
     6061 gatcctcatg acggtattcc tccttcattt ccgaattgct ggcaagattc aggttgatat
     6121 gacagtagcc gttcggaact ttgtccagat agtcctggtg ataaagttcc gcttcataaa
     6181 aaatcggagc catttttact attgtctgaa ttggatctat gtatttgccc tttattatat
     6241 tcacatagtt ttgtgatttt tccatgtcgt atggcgacaa taaaaagatt ccgtttctgt
     6301 actgtctgcc gatgtccggt ccttgacggt ccttttctgt tggattgacg atccggaaaa
     6361 aatgatccag aagtgtttct aaagatatct tggcttcgtc ataggctatt cttaccgcct
     6421 cggcgtgacc ggagccatcg caaacttcct tgtatgtcgg gttttccgtg tatccgtcaa
     6481 tatacccgac agtcgtttcc ataacgccat tcaagcgttt gaaataggct tcgactcccc
     6541 aaaagcatcc gccggcgaag atgatcgttt tcatgatttc acgtcatccc ttccgatgat
     6601 tttgtttacg acgtagcttg cgatgataag tcccgcagcg gcgggtacga aagcggtcga
     6661 accgagcggt tttgcttgtg ttgtggcagt tggtttttcc ctagaatata caactggtat
     6721 ctgatcacag ataccgaatc tgccaagttt ttgcctcatt gttctggcaa gcgggcatac
     6781 cgatgtttcc gaaagcatgg caatctcgat catttccgga tgaaacttgt tggcaaaccc
     6841 catgctggat atgacattga ttcgtttttt ccggcaattt tccaaaagcg acgcttttgt
     6901 ttcgacatcg tcgatggcat ctattaccca catgggattc agggccaaaa aatttgcaat
     6961 attatccttg gttgcccttt gaaaataaat atcgatttta agagcgggat tgatatcgtg
     7021 aagccgtttt tcggcaattt ctacttttgg cgagccgaca gtcttaattg tcgcaaatat
     7081 ctgtcggttg atattggatt cctcgatcag atccgaatcg atcaatgcga tatgaccgat
     7141 gttgcttctt gccagggctt cggccacata agatccgacg cctccgattc catacaccat
     7201 gacaaaagca tttgccagtt tattcattcc ttcgtcgcct ataagcagac gaagacgctc
     7261 gaaagaaggc atattgtcct cctaacaaat atggctgcga ccgcagccat ttttatttat
     7321 acagattgaa tttgaaatat ttacgtccca gcatgattag tagatagagc atcagggcga
     7381 ggatcacaat cagaaaattg gcgatatact gacgatcgaa tcccaagtaa tcgtacataa
     7441 cggcaaatcc gagcaatccg aaggcgctga tcatcatcaa atagataaac tcgttgcgat
     7501 acgatgggcg tacccgacgc ttcagatggt ctcttttgag gaataggatt ccggtgccga
     7561 tcccggtaag acagagaacg gctatcgcgg cccagaacat gaagacgaga ttgttgaaag
     7621 atccgccgat cggaaacaga taggtcacaa ccagaatgat tggcaacaaa aggatcattg
     7681 tcgcatagaa cacgatcatc ctgatcgcaa actcggaata gatccttact tttttgccgc
     7741 ttctcatgtc ttggtcttac ctccccctcc ttccggcgaa agctcgttaa gttgcttctg
     7801 ttcgcggaag aaattgtaga atacccggat cgctgcggcg atcggggagg cgaatacgac
     7861 gccaaaggtt ccgaaaaggg caccgaagaa taaaatcgat ataatgatga caagcggatg
     7921 catctccaac tgtttggcca tgatgatcgg ctggaagatg tttccttcga caaattgcag
     7981 accgatattc acggctagca cgataagcgg cccgggagcc actcccgata tcgccgcata
     8041 ggcgatcgga ggaatggcgg cgatgatcga accaaaatac ggaatgatat tggttatgcc
     8101 taccaaaaga ccgaatgcga aatagtattt gagtccgatc agcttgtaaa cgattgtggc
     8161 gacagcaccg atcgcagcca tcaagatcaa ctgtccgcgc aggtaggcgc caacagtctg
     8221 attcaatctg gatccaagat cggcaacccg tttctcgtgc ttttgcggaa tgatggaacg
     8281 caggcggtcg ccaatcattt cataatcaag cagataatat agcaacatga tcggtatgat
     8341 cgctacggct gtcagaatcg gaatcaaagt atcggtcatc cctgctagga aagtcggaat
     8401 cagggagtca agccaggtat tgagaatatc ggtgccggcg acgtaattga agatcttgtc
     8461 gtatatgtcg gaaccgagga tgaagttgtc gcggagttcc gcgctcgtga ggtagccgat
     8521 gatcttctga aagtcgtcat tgaagaaggc agtaatttct cttactaccg ccggtatgat
     8581 gaaatagaag aaagcggcga taatagcgat catcagcaag aaaactatgg ccagcgacag
     8641 ccatctcgga ccgacgccct tcttttccag atatttgaga agcggatgca tgatcaaagc
     8701 gaggaaatag gcgaaaacga ccggcactac gacggtcttg atcgccttga atatccagtt
     8761 ccacacatcg gaaaacagcg tagccatgaa aatgacggca agcgtcagca aggctatcgc
     8821 caggtacttg attatgcggt tgagaagttc gtcggaaatc ggcttctttt tcacctttca
     8881 cacaccttcc ggttcttttt gattatagca taacaaaaaa caaaaataca ggtatttcac
     8941 gaaaactcag caattgccac cgcagctcgc gcaattgccc gagcaactct cgtgttcctt
     9001 gcggatcagg gtgacgagtt cggcatagag tttattgaag gggatttctt cctgtactcc
     9061 ggttttggcg tttttaacgt taacgacgcc ttttttgtct tcgtccgatc cgtagaaaag
     9121 atagaacatc ggattgaatt tctccgcttg tttgaactgg gctttgagtg tctttccgtc
     9181 atagtcgtgt tccacgacaa gtccaccgtg tcttaacatc tcgatgatgg gaagactttt
     9241 ttcccgggct tcttctccga gtgaaaccat gaaagcatgg atggaatcgg gaatttcctg
     9301 ttcttccaaa gcatcaaggg caagcaacaa tctttcgagt ccgaaagcga atccgacgga
     9361 cggaaggctg ggaccgtcca gggattccga cagtttatcg tatctaccgc cgccacaaag
     9421 cgttttttgg gatcccaatg tttccagatt gctgtcgatt tcgaaaaccg tatgggtgta
     9481 ataatcaagc ccgcgcacca gactgggatc gacgacataa tcaatcccca tccggtccag
     9541 attggcaaga acctgtcgga aatgggcttc cgccttttcc gaaagactgt ctttcggttt
     9601 cggggcattt ttgagaatcg tcgagtcgct gtcgactttg caatcgagca ctctcagggg
     9661 gttctcaaga tatcttcgct ggcaatcctc gcataagccg cctatctgcg gagtcaggta
     9721 ttccttcagc acctgtttgt agttctcttt gctagaggca tcgccaaggg tgttgatcct
     9781 gacgacgatt ccgggaatct gcagggcttt caggaaagtg acgctataag cgattatttc
     9841 cgaatcgaca tatggcgatt cggagccgat ggcttccgct ccgaactgat gaaattgtct
     9901 ttgtctgcct ttctgaggcc gttcgtagcg aaacatcggt ccataataga aaagtttcaa
     9961 aggctgaggc atggttgcgt ataacttgtt ctcgatcacg gcccggacaa tcgatgccgt
    10021 cccttccgga cgcagggtga tcatgcggga tccgcgatcg gcaaaatcaa atgtttcttt
    10081 cttgacgata tcggtcgaat cgccgattcc gcgatgaaaa agttcggttg cctcgaaaat
    10141 gggcacccgg atttcctgaa agttgtagat tcgggaaacc tgacggatca catgctcgag
    10201 atcttcccat ttttttgcct catccggtag gatgtcgtgt gttcctttga tttttgtgat
    10261 catgttatct cctttatgaa aaaatcgtcc ttgttggaaa aggacgatat cctccgacaa
    10321 gatcagttag agggatgttt ttcgttttca gccaaaatat ttgtcgctga catgttcaac
    10381 gacatattcg acgagatcgg aaacgtcttt catcttcatg gcggcttcat cgctgatcgt
    10441 gatcagaaat tcgctttcga tctgattgaa gagttccaaa gcgtcgaggc tgtcggcgcc
    10501 gaagtcatcg gcaaggcctg catccatgac aactttttcc ggtttcacac ccagttcttg
    10561 gatgatgatt tccttaattt tctcgaacac gacttgcttt ttgtccatcg ttttcctccg
    10621 tggttgtggc tatttcatat ctcaaatggt attatattaa atttttgact ttaagtcaaa
    10681 caatacaggc atttttcctc tatctttcat agattatcgt aacggggccg tcattggtta
    10741 gttcgacttc catgtgggct ccgaaaacgc ccttcttgac ttccttgcca atcagggcgg
    10801 aaagccggga aacgaattga tcgaaatacg cttgtgcctt gactggctcc atcgccttgg
    10861 tgaatgacgg acggtttccg tccgatatgt ctccgtaaag cgtgaattga ctgacggcga
    10921 gaatatcacc ggatatatca atcactgatt tgttcatttt gtcattatca tccgggaaaa
    10981 tgcgcaattt agcgatttta ccggctacgt attcaatttc tgtttctgtg tcattacttc
    11041 ctattcccac taaaagaagg tatcctttcc caatcgagga aatgtttttc ccttccaccg
    11101 agaccccggc tttggcaact ctttgaacga cgactttcat cctaacagat cctttcgatc
    11161 gagaaaatgc cgcggatttt ctggatattg gcaacaactt gatccaattc gacgacattg
    11221 ccgatttcca gtttcatcct gatgatacct tcgctggcct tgttggtttg cgcgcttacc
    11281 tgatttattt tggctttcga cgaagtggcg acattgatga tctcggccaa aatgttatcg
    11341 cggttctgaa cgatgatctt caggcttgtc tcataacgct tcgtgacgtt cgatccccaa
    11401 tatacctcga tcaatctggc cttgtcgtaa tagtcaaggt tgttgcagac gtcgcggtga
    11461 acgataatac cggagttttt tgacacatat ccgacaatat tatcaccaag aatcgggttg
    11521 caacagttag caagtcttac cgagggattg gtgagacctt ccacaatgat gttgatatcg
    11581 ttatgcaagg ttctggtctt ctgctggcgg cgttcgtccg gcgtatttat cttctcgatg
    11641 agttcttctt cgcttatcgt ttccttgccg gtcatcaaat tgacagctcc gaccggcgaa
    11701 atcacgtttt taccgatttc gtagtaaaga tcctcttccg atttgacacc tttgttgccg
    11761 aatagatccc gacacagttt atcggttatg tcaatctgga ctttccgact ttgaagttcc
    11821 tgttcgaact cctgtttccc ggtttcgacc agatattcac gtttctggcg attgaggaaa
    11881 ttcttgattt tgcttctggc gttctggctt tttgccattt tcagccagtt attgttgggc
    11941 ccgacggcat tctggctggt cttgacttcg accatgtcac cggtttttaa agtgtaatcc
    12001 agcgattgga ttttcccgtt gacgatcgca ccgaccattt tctcgccgat cttggtatgg
    12061 attctaaaag caaaatcgat tggtgtcgct cccgctggaa ggtcgataat gtcgcctttt
    12121 ggcgtaaaca cgtagacgtt tgcatttaaa atgtcatcgg tgaaaatatc gaggatttct
    12181 tcgtcggaag ccgattcttc ggtgtacttt atcagttcgg aataccatct cagtttggaa
    12241 gaaacgatgt cctcgatgtt cttgcggcta tggcttccgg attgcgactc tttataggcc
    12301 cagtgagcgg caacgccttt ttcggctact ttgtccattt ccctggttct gatctggatc
    12361 tcgtagatct tgcctttgtt tacgaccgtc gtatgcagcg attgatacat attcggtttt
    12421 ggcatagcga tgtaatcttt gaacttgcct ggcacaggaa taaatttcga atggataatg
    12481 ccgatcgccc ggtaacaatc gctgacggtg tcaacgatca cacgaagggc caacaagtca
    12541 ctgatttctt cgaaatccag gtgtttcaat tgcattttgc ggtatacgct ctgaatattc
    12601 ttgattcttc cggatatctc gaaatggaaa tcctcgcttt tgagcagttc ttccagttcc
    12661 acttccatag tcttgatgtc ggcttctctg gttcccttct tgtctttgat cagacgcgcg
    12721 atttccaggt attcgttcgg atacagatat ttgaaagcca agtcctcgag ttcggctttc
    12781 agccggtata ttccgagcct atgggcgata ggggcgtaaa tgtccagggt ttcccggctg
    12841 atccttattt gtctttcatg gtccagatag gaaagggtac gcatgttatt gaggcggtcc
    12901 gagagtttca cgaaaattac ccggatgtcc ctggccatcg ccaagaccat cttctggtgg
    12961 tttttggctt gttcggtgac tttgttggaa gttccttcat attgcaacaa atgcaacttg
    13021 gtgacgccgt cgacaatgtc ggcgacttcg tccccgaatt tgcttttgag ttcctcgtag
    13081 gtaccgctag tgtcttcgag aatgtcatgc aataatccgg cggtgatcgt gttgggatca
    13141 acacggtatt ccgccaaagt gacggccacc tggaccggat gggttatgta gggatctccg
    13201 gatttccggt attggccgtt atgtttgtcg taagcatagt caaaagcgcc tctgatgaaa
    13261 tcgaggtgct caggtttgga aagatagggg gatatggctt ccgcaagcgg caggtacagc
    13321 tgttcgcggt ccattggcat tctcctttct tctgactgtg ctttagtatt tgatcaacgc
    13381 tttgatggga tagccctggc atttgccacg gccgccaagg tcaacaagtt caatcaaaaa
    13441 gcctagcccg acgaccacgc cgccggcctg ttcgaccaat ttgatcgtgg cacccatggt
    13501 gcctccggta gctaaaagat catcgacaat caatactttt tcgcccggtt ggatggcatc
    13561 cttatgcatg cagagagtgt tcttcccata ttccagttca taatcgtaac tgatggcttc
    13621 ccggggaagc ttacccggtt ttctcaccgg tacgaatccc acctgaagtt gacaggcgac
    13681 gggagcgccg ataatgaagc cgcgggcatc cggcccgatt actttggtcg cttcgaggtt
    13741 tttgccgaat tcggccagtt cgctgacagc ggcgtgaaag gcttctccac tgccgatcag
    13801 gggagtgatg tccttgaatt ggattccggg aatgggaaaa tcgggaacgt ttttaatgta
    13861 atcttcaaat ttcattgtat gcctccaaat gggttattat gatatttttc gaatctcttt
    13921 gacttgcggc gtatgtcttt tcgatcaaat cggcttttac ccgccaatga ttgtatttgg
    13981 cggactgata aagatccttt ttgggcggtg cgtccaccgg tatgtaatgg ttgccttcca
    14041 cttcgacgaa tccaaggtcg cggaaaatgt ccaagatgat cgcggtttct tcggtgtcaa
    14101 gtccgaaaaa ccgggcgaaa tgatccaaag tctcactttg ttcccggatc atgatccggt
    14161 aacagcgtcc taaatagtcc ttgttcagca ctgctttcaa cggttgggaa aatgccctgg
    14221 gcaaaatgat gacagtaccg tctttctgaa ccttcaaacc tccggcgatc gtcctgtcgg
    14281 atattaaaac agcgtctttt tgaagaactt tggcaagctg gtcgcgccag aacgagacat
    14341 gacggaaatc aagtgtttgg aaatctttgc attcgatgtc cttggccgtg atctggatca
    14401 cggttttctg gagccaggtg ttgattccga ttccgccaac gacgtttatc ctgtcgcccg
    14461 tttcaagcga ataaaaatag tctggattgt taaaggcaag caaatcgaat aatttcccat
    14521 cggattcgac ctgcaatttg gtatgttggt cgcggatcaa ttgttttttg cggacataaa
    14581 gtccggtaaa aaggaaagta gcggtgaaaa acgagaaatc ctgtagtttt cgcacggttt
    14641 ccaaggaaac ctcggccaag tttatcgcca tatcgacttt tagcggcgga gtctcctgat
    14701 gggtggaaat ctgattcaac ctttggcgca gaatttccag atcggccgga tttatctgca
    14761 gtccggcggc ttgttggtga cctccgtatt tcagaatgac atcttcgtta acctgaagca
    14821 ttttcaggat gttgtcatcg ccgaaagccc gagccgatcc tttggctttt ccggattcgt
    14881 cgatcgtgaa gacaatggct gattttt
//
LOCUS       DAOPJL010000026        14860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_4233_length_14860_cov_7.026410, whole genome shotgun sequence.
ACCESSION   DAOPJL010000026 DAOPJL010000000
VERSION     DAOPJL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14860
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_14860_cov_7.026410"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLP02_04375"
     CDS             complement(<1..382)
                     /locus_tag="PLP02_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42712.1"
                     /translation="MITSIDLDDKIIETTIDSEGQLISFINLGASITRWRTKDGTDIV
                     PAYREFSQYQMPLEPGMYLGATIGPIAGRIRDSAFDFGNRHYAMDPDYAGFLHSGKQS
                     MAFMYFEIVRIGKDDRGEFVIYRLD"
     gene            complement(366..1085)
                     /locus_tag="PLP02_04380"
     CDS             complement(366..1085)
                     /locus_tag="PLP02_04380"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF42713.1"
                     /translation="MENKPEILYEDNHLLVALKPPGFLSQPGKKNTADMLTYLKEYIK
                     NKYGKPGNVYLGLVHRLDMNVGGVMVFARTSKAASRLSEAIRERNFWKEYLAVVEGKL
                     DTGTRIVLEDNLVKNSVSLKGQISESGKKSSLSYVCLANKKIDEKWISLVRISLGSGR
                     FHQIRVQFSSRNHPIMGDTKYGSQLRIGSNSLSLWAYHLAFLHPVTGEKLSFQSLPSI
                     GGFAYFQDIIVKTGPVDDNIN"
     gene            complement(1091..1888)
                     /locus_tag="PLP02_04385"
     CDS             complement(1091..1888)
                     /locus_tag="PLP02_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPF42714.1"
                     /translation="MFNLPDEITTVIGIAFIFLMTSAGAAMVFFFRKTMSTLAKQLFL
                     GFAAGIMIAASVWSLLIPSIEESEANGVIAWIPAAVGFACGGLFLLLVDNLIPHLHLG
                     EDHPEGLKSSLKRSTMLVFAVTLHNIPEGLAVGLAFGLALYNGSAIAITAAFGLALGI
                     GIQNFPEGAAISLPLRQEKVSAGKSFLYGVLSGAVEPVFALLGIVLATQLTALMPWFL
                     AFAAGAMIYVVVEELIPESTIGEHSNIGTLAVMAGFIIMMILDVALG"
     gene            complement(1899..2975)
                     /locus_tag="PLP02_04390"
     CDS             complement(1899..2975)
                     /locus_tag="PLP02_04390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42715.1"
                     /translation="MSLRSAKAEDVEKAIVLASETFDKSMGSLFPLLLSQANRNHMFV
                     AAYKNEVCSMVNYYPSVVRLIDCDIMAASIGSVATSPLYRRRDLASKLLSIAKTQMER
                     EGIDLVIISGEGGIYDRFGAKRAGNVNGYLIDIDNLPLNDVVTIKDYDEKYLFDLKTI
                     YDREPIRYLRNDQEFAMLIKGSTYPEPFAETHLEIIFKKGKPVAYVNLKKSDDPDELL
                     IREIAGPREDILSSLRQIMAKYGHRQIILPVDISDPINSLMDKDAAMKTDQYASFTVL
                     NWERFFKKLQPYFQTKGCIVTIAEKDGKQTLEVNGSRFETDNCRDLISIVFGEANSER
                     TGIPVSFSEVFPIPFVWTNNMNYQ"
     gene            complement(3009..4220)
                     /locus_tag="PLP02_04395"
                     /pseudo
     CDS             complement(3009..4220)
                     /locus_tag="PLP02_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(4307..5623)
                     /gene="aspS"
                     /locus_tag="PLP02_04400"
     CDS             complement(4307..5623)
                     /gene="aspS"
                     /locus_tag="PLP02_04400"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HPF42716.1"
                     /translation="MQKLYFKDLPSHYGEEVRIDGFVDKVRDLQYVQFVVLRDSTDKI
                     QITIEKNEDNTAINQVVSGLTKESTVSVKGLLMENPHVKLRGMEVIPSDFIITSLAAD
                     ELPIDILDYTNKSNKELRLDNRFLDLRLEKNYLIFKAQTIAEMAMREYWTNHNFIEIH
                     TPKILGTASESGAEVFKLDYFGRTVYLAQSPQFYKQMAMASGFNNVFEIAPAFRAENS
                     HTAYHATEFTSVDCEISWIDSHHDVMDMEEAVCKAVMNKLADEIGDEYERLYQKKIEK
                     DIPDFPRIKFADAKKIINEKYHYIGEKDYDFDRREEELIGIYAKKEFKSDYVFVIDYP
                     FAARPFYHMLDEKGDTKSFDLIYKGIEITTGAQREHRIDILQKQAVEKGLSLESIDFY
                     LNFFRYGVPPHGGYGYGLTRFLMRLFEAESIRDVTFLYRGPNRVNP"
     gene            complement(5706..6755)
                     /locus_tag="PLP02_04405"
     CDS             complement(5706..6755)
                     /locus_tag="PLP02_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPF42717.1"
                     /translation="MTVFLALADWERAFLSGAIAATLILAYLWQKKLVSKSENAFRKG
                     WFILIYLADAVVFFAALAGVMLLWNFDFATLFDDFFTNTATFLLDKIGAIIGSIITVF
                     LSVAIVSFSKHAFGKVGLKAGPLQKRRMTIAKVSLSIVKYTVVIIVVLVILALWGVNV
                     VPALAGLGIVGLVVGLGAQNFINDLISGFFIIFEHHFDVGDIVEIEGFKGEVSEIGLK
                     TTRIRNWKGEVKIIANGNITNLINYSRNPSLAIIDFGIAYGEDIEKTVALMKAELPKL
                     KDEIEEIIEAPQVLGVIDLADSAVVIRVIVKTMTEKHYGVERQTRQRIKELLDQNNIE
                     IPFPQVVVHQAKSGE"
     gene            complement(6885..6961)
                     /locus_tag="PLP02_04410"
     tRNA            complement(6885..6961)
                     /locus_tag="PLP02_04410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6925..6927),aa:Pro,seq:cgg)
     gene            complement(6964..7052)
                     /locus_tag="PLP02_04415"
     tRNA            complement(6964..7052)
                     /locus_tag="PLP02_04415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7015..7017),aa:Ser,seq:cga)
     gene            complement(7095..7168)
                     /locus_tag="PLP02_04420"
     tRNA            complement(7095..7168)
                     /locus_tag="PLP02_04420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7134..7136),aa:Gly,seq:tcc)
     gene            complement(7273..8199)
                     /gene="rsmH"
                     /locus_tag="PLP02_04425"
     CDS             complement(7273..8199)
                     /gene="rsmH"
                     /locus_tag="PLP02_04425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPF42718.1"
                     /translation="MAGHVPVLLEEAIEYLEVRHNGIYVDMTTGGGGHSKRILEKLDG
                     TGHLFCFDQDEFALGEAKKNLARYANVVFINDNFVNCRKQLSSLGIEKVDGILYDLGV
                     SSYQFDMPDRGFSYRQEGPLDMRMDKTNPITASIIVNTYPEKELGNIFYRYGEDPFAR
                     PIARKIVEYRMKKTIETTSELVDIIKSALPQAILHKKGHPAKQVFQALRIVVNDELGV
                     LEKSLFDSVDILKPGGRIVVITFHSLEDRICKDMFKRLSTIEIPKGLPVIPDEKPVLK
                     LVTRHVVKPSDDEINDNNRAHSAKLRAVEKTL"
     gene            complement(8266..8466)
                     /gene="rpmF"
                     /locus_tag="PLP02_04430"
     CDS             complement(8266..8466)
                     /gene="rpmF"
                     /locus_tag="PLP02_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPF42719.1"
                     /translation="MAVPFRKTSKSVKRKRRTHFKLSAPAMVVCPNCGELTLAHQVCK
                     KCGYYKGVLVKEPKPEKTEDEK"
     gene            complement(8486..8962)
                     /locus_tag="PLP02_04435"
     CDS             complement(8486..8962)
                     /locus_tag="PLP02_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42720.1"
                     /translation="MKWSTFELRKLHRTDNRFAYVCDCSDLIGENDEDLAGVSPVNVS
                     GTFEYDEIGERYIFSIDIACHLTMLCAITLDEVDVPLNFHSILEFASVVKDDFTLPIE
                     GTTIDLDPFIWAEIMVEKPMRVLSENAYRNYREEIIELDEAEKMADNPFAKLQKKN"
     gene            complement(9028..9804)
                     /locus_tag="PLP02_04440"
     CDS             complement(9028..9804)
                     /locus_tag="PLP02_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPF42721.1"
                     /translation="MLIKIMKHFVPIPDITKSSRFCFVGPHPDDIEVGAGSTVSMLKR
                     LGKEIMFVIVTDGGSGTDDPSISVDRLVATRKEEAKQSAEKLGVDKVVHLGFPDGGEY
                     QAWNVAKKIAEVLIDFDPDVVFCPDPRMPSEIHPDHLKTGEAAGTASLFSSFPLMASQ
                     NGVAYGNTGNFRYRILAYYFTNRPNRIMKTTKTDFVNQLDSLKLHKSQFKNKEEFGTL
                     SMYLKLRQKEMGFKRLRCSGEGFFVLGPVHQHCFTEINRY"
     gene            9948..10961
                     /locus_tag="PLP02_04445"
     CDS             9948..10961
                     /locus_tag="PLP02_04445"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPF42722.1"
                     /translation="MTVKYTKEEISARIGELTDPSRNGRSLAELGAIKHIGFDEDKQG
                     VVLLIEVGKKDNETTAPITRQIAKIIKIDMGYPSLVVEFEQARPKALTKTVKIIGIAS
                     GKGGVGKSTVTANLAYALTSLGKKVGIIDADIYGANMPKIMECVDENLMPAPNEKLYP
                     LEIDKIEMVSTEYFVEKDHALMWRGPLLSKVLKLFFEDTLWNPELDYLLIDLPPGTGD
                     VMMDIKNFVPDCKMVLVTTPHPDASHVAIKAGFAAISLKEDLLGVLENMSYFETEGKQ
                     YNIFGEGGGKLVAEKLGVPLIGRIPIGQPAGKKHSVFGETEPIGLIYLGIAKKIMDLY
                     EDN"
     gene            complement(10964..12223)
                     /locus_tag="PLP02_04450"
     CDS             complement(10964..12223)
                     /locus_tag="PLP02_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42723.1"
                     /translation="MQNRLQNEVEKRKEEMYQFYYRGIGVSIKKRRLELKMTQEALAK
                     GICSNTYISKLENNAIVVNRENLYLIMEKMDLPQDTIGFPEDMVSILEKSFDYFVRDE
                     KEKYRRLYEKISEYKFGILVVLARFGYLVMTERLEEAKPLYIEIFKYLNSLEEYGLAI
                     FSIYAAMYNVLLFNFEQAKAIIDSVEKISCVNQQAGAMIAFVKYIIYGHLYLFSPARD
                     SYEAAMSYFIKTHNLSRIADLMVFRNIFLIYGGSFDKVAFDEKDLMFLSGWIINYYLT
                     VLSICDDKPERFREFTDRNTRFYSDYLYILAASYRERKLDDEYKKTIAELENATAFYK
                     TEKTIDYIRCLELEKDKNSLAYKDYLVNFVLPYLMNLQNICYTGVVTDRVIEILHNHK
                     RYKDALGYRNKYRSYVQKLQGIKNNQG"
     gene            complement(12392..13024)
                     /gene="nth"
                     /locus_tag="PLP02_04455"
     CDS             complement(12392..13024)
                     /gene="nth"
                     /locus_tag="PLP02_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPF42724.1"
                     /translation="MNARSKARFVYDKMGELYPDAGCELNFRNELELLVSIMLSQQAT
                     DLSVNRLTTSLFQKYQKIEDYAKATETEMQNDIKTIGLYRNKAKNLIAMSKEVIARYE
                     GIIPKNRELLEELPGIGRKTAGVFLAEWMHEPHFPVDTHVKRVSWRLGFSKEKDDPDK
                     VEAKLEKLYDPSEWITAHHRFLFFGRYHCTARNPRCKECILIKECKHPLL"
     gene            complement(13021..13686)
                     /locus_tag="PLP02_04460"
     CDS             complement(13021..13686)
                     /locus_tag="PLP02_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HPF42725.1"
                     /translation="MDITLIKRLIDEGIIDFEKLILRNYLDAGLSETEAFVIIELYRQ
                     MLKGNTYLSPAKIGKNLSLEKEDLLGILDNLIKRQYVTIELKKGKNGKETEVFLLDGT
                     IQTIMANYQKEIKNEIIDKPKKYATNAEEIVDLLETEFKKQLTPLEIEIIQKWLDEDK
                     FDIIDIKKAVLDSIKAGKSSLSYVDGILLRRRQKAKKAVETTYKAENSEALKQFYDSW
                     DKK"
     gene            complement(13692..14390)
                     /gene="rnc"
                     /locus_tag="PLP02_04465"
     CDS             complement(13692..14390)
                     /gene="rnc"
                     /locus_tag="PLP02_04465"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPF42726.1"
                     /translation="MNEVKIRQLEARFQLRFNDFGLLIRAMTHSSYANEHNTRSNERL
                     EFIGDAVLDLAVGKYLFEHLDEDEGVLTKKRAQDVCEGSLFEYAKSISLGDYLLLGKG
                     EEKNSGREKPALLADAFEAFLGAIFLDKGFDEVQKVLEIVVYPRIKESLDREDSDYKS
                     KLQELVQSDKRTLNYVIVSESGPAHDREFVARVYMDDDIIMGCGKGKSKKEAEQNAAK
                     ETLDKLASGIIDKE"
     gene            complement(14383..>14860)
                     /locus_tag="PLP02_04470"
     CDS             complement(14383..>14860)
                     /locus_tag="PLP02_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPF42727.1"
                     /translation="GTEDGKGREIDHEVFSLFKETNLFPFYGNVEPKSIFDPPCDILI
                     SDGFTANIVMKTMEGTAKGMGTFLKMALMENFTGKIAALIARKNLAHFKKMVSPEEIG
                     GALIYGLYRPVIKAQGASKAYGFSNAIRQAAAVVRNGVFPKVEKYIQETTGEAKDE"
BASE COUNT         3885 a         3502 c         3162 g         4311 t
ORIGIN      
        1 aatctagtct atagatgaca aattcgcctc ggtcgtcctt gccaatcctg acgatttcga
       61 aatacataaa agccatgctt tgttttccgc tatgcagaaa accggcatag tcgggatcca
      121 tggcataatg cctgttaccg aaatcaaatg cggaatcgcg gattctcccg gctatgggac
      181 cgatggtggc tcccagatac ataccgggtt caagaggcat ttgatactgg gagaattcac
      241 gatatgccgg aacaatatcg gttccgtctt ttgtccgcca gcgagtgatc gaagctccca
      301 aattgataaa tgatatcaat tgtccttccg aatcgatggt ggtctcgatt atcttatcgt
      361 ctaagtcaat tgatgttatc atctaccggt cctgttttta cgataatatc ttggaaatat
      421 gcgaatcctc cgattgaagg aagcgattga aatgacagtt tctcgcctgt aacaggatgc
      481 aaaaaagcca agtgatacgc ccaaagcgac aaactgttcg atccaattct caattgcgac
      541 ccgtatttcg tgtcgcccat gatcggatga tttcgactgg aaaattggac tctgatctga
      601 tggaaacgtc ccgatccaag acttattctg actaatgaaa tccatttttc atcgatcttt
      661 ttgttggcaa gacatacata cgacaaactg gattttttgc ctgattccga aatctggccc
      721 ttcaacgaaa cgctgttctt gacgagatta tcttcgagaa caattcttgt tcccgtatcg
      781 agtttgcctt cgacgacagc cagatattct ttccagaaat ttctttccct gatcgcttcg
      841 gaaagacgcg aggcggcttt tgaagttctg gcaaaaacca tgacaccccc gacattcatg
      901 tcgagcctgt gaaccagacc gaggtagacg ttgcccggtt ttccgtattt attcttgata
      961 tactctttca aatatgtcag catgtccgcg gtgttctttt tcccgggttg cgacaagaat
     1021 cccggaggtt tcaaagccac caggagatga ttgtcttcgt aaagaatctc cggtttgttt
     1081 tccatactaa tcatcccagt gccacatcaa gaatcatcat gatgataaat ccggccatga
     1141 cggccagagt accgatattc gaatgctcac cgatcgtcga ctcaggaatc agttcctcaa
     1201 ccacgacata gatcatcgct ccggccgcaa aagccaaaaa ccatggcatc aaagccgtga
     1261 gctgtgtggc aagcacgata cctagtaatg caaaaaccgg ttcgacagca ccgctcaaga
     1321 ctccgtacaa aaaggacttc ccggcagata ctttctcctg acgtaaagga agcgatattg
     1381 ccgctccttc gggaaagttt tggattccta taccgagagc gagcccgaat gccgcggtaa
     1441 tggcgatcgc cgatccgtta tacaatgcca agccgaaagc caaaccgacg gccaagcctt
     1501 ctgggatgtt gtgaagcgtc accgcaaaca caagcatcgt cgaacgtttt aaggaacttt
     1561 tcagtccttc gggatgatct tcacccaaat gcagatgcgg gatcaggttg tccaccagga
     1621 gcaagaacag gccgccacat gcaaatccga cggcggcggg aatccaggcg atgacaccgt
     1681 tcgcttccga ttcttcgatc gacggtatca gaaggctcca taccgaagcg gcgatcatga
     1741 tgccggcggc aaaacccaaa aagagctgtt tggctagggt actcatggtt ttccggaaaa
     1801 agaaaaccat tgcggccccg gcggatgtca tcaggaatat gaacgctatc cctatgactg
     1861 ttgttatctc atcgggaaga ttgaacatcg gtttcctcct attggtaatt catattgttt
     1921 gtccagacaa acgggatcgg gaacacttcg ctaaaagaaa ccggaatccc ggttctttcc
     1981 gaattggctt cgccgaaaac gatgcttata agatctcggc aattgtcggt ttcgaaacgt
     2041 gaaccattaa cttctagcgt ctgtttcccg tctttttccg caattgtgac gatgcatcct
     2101 ttcgtctgga aatatggctg taattttttg aaaaatcttt cccagttcag caccgtgaaa
     2161 ctggcatatt gatcggtctt catcgccgcg tctttgtcca tcaaagaatt gatcggatct
     2221 gatatatcaa ccggtaatat tatttgtcta tggccgtatt tggccattat ctgacgcaat
     2281 gacgacagaa tatcttctcg cggtcccgct atttctcgta tcagtaattc gtcaggatca
     2341 tctgattttt ttaaattgac gtatgccacc ggttttcctt ttttgaatat tatttctaga
     2401 tgggtttcgg caaacggctc tggataagta ctgcctttta tcaacatggc gaattcctga
     2461 tcatttctca gataacggat tggttctcta tcatatatcg ttttcaaatc gaaaagatat
     2521 ttttcgtcat aatctttgat tgtgacaaca tcgtttaacg gcaagttgtc gatgtcgatc
     2581 agatagccat tgacatttcc cgcccttttg gctccgaaac gatcgtaaat tccgccttcg
     2641 ccggaaatta tcacgagatc gattccttct ctttccattt gtgttttggc aatcgaaagc
     2701 aactttgaag ccaaatcacg gcgtctatac aaaggcgatg tggcaaccga tccgatcgat
     2761 gctgccataa tgtcacaatc tatcaatctg acgacggaag gataataatt gaccatcgaa
     2821 cacacttcgt ttttgtaggc ggctacgaac atatgattgc gattagcctg tgacaaaagc
     2881 aacggaaaca gcgatcccat gcttttatcg aaagtttccg atgccagaac aatcgctttt
     2941 tccacatctt ccgcttttgc agatcgaagg ctcaaatcgc taaacgacga acaatacttt
     3001 gaaaaatcga cgactttgtc gtaacgtcgg gaaatttcgg ccgcaaatgc caacaggtga
     3061 ctttcaaccg aagcttttac atcggctttt tccagttttt tgccttctga caccatttta
     3121 acgaaatcat gcataattcc gtgatcgccg ccaccatgac cggcgatatc gtcaccattg
     3181 aatcttacgg ttatgggctc ctgaccgaat ttggccagta ttaactcgtt tttctccata
     3241 tggccgcgga tctcgccctt ggttcccatc agtttgatcg ttcgcatgtt ttcgttagtg
     3301 aatgccgaca tcgtgaaaca ggcggtaacg ccattttcga attcaatggt cgtcgactgg
     3361 tgatctacca catcgttgtc acatctgtat acgcagcgtc catagggact ggtaggaagc
     3421 actttcaaaa ggctttcgtc cgacatgtcg ttcgataccg gcaatttcat ccatatcggg
     3481 gcattcaagt atactttcgg cgcaaagaac acgcattcgt cctgagccgg acaaccattc
     3541 atacagtaat cgggacttcc ttcgggagcg ttttttttac agaaatgctt tagatttcca
     3601 aaagacgcta tctttgtcgg atgagatgcg gcaaaccata tcaacaaatc gagatcgtgg
     3661 catgatttgg ccagaatcat cggacttgac agcgcttcgt tccgccagtt gccgcgcaca
     3721 tagctgtggg catagtgcca gaatccgacg ttctcgttgt gctgtatggc cgtcaattcg
     3781 ccgatttctt tggcttccag ccattctttg atttgagaaa aaaattcggt atagcggaga
     3841 acatgtccga tcatcagaat aacgccgttt ttcgtcgcgg cttcttccaa ggcaatacag
     3901 tcttccggtt ttacggccat cggtttttcc aaaaaaacat gataaccttt ttccatagcc
     3961 atgatcgcgg gttccagatg catggaatcc tgtgtgcaga tgaaacaaac atccgcaaaa
     4021 cgctcttttt ccaaaatcgg aaggtaatca gaaaattggt tttccaaaga aagccggtgt
     4081 tgcaaggcaa aatatcccct tcgcacggga tccggttcgg ccacgccgac gaatgtaatc
     4141 tcatcttgat gatgaagtgc ataatctccg tatactccga tgcctcttgc tccggctccg
     4201 atcatgattg ccttgaacat aatttatcac ctgctcgata tccattttat cacaattaac
     4261 cggatttatg accacgctaa gaaaaaaacc aggcaatatg cctggttcaa ggattgactc
     4321 ggttggggcc gcgatacagg aaggtgacat cccggattga ttcagcctcg aaaaggcgca
     4381 tgaggaaacg ggtgagtccg tatccatagc ctccgtgggg cggtacgcca tagcggaaaa
     4441 agttcaaata gaagtcgatt gattcgagac ttagtccttt ttccacggcc tgtttttgta
     4501 agatatcgat ccgatgttcc ctttgggctc cggtcgtgat ctcgatgcct ttgtagatca
     4561 aatcgaaact cttcgtatcg cctttttcat ccagcatgtg gtaaaacgga cgggcagcga
     4621 acggatagtc gatgacgaag acgtaatccg acttgaattc tttcttggca tagattccga
     4681 tcagttcttc ctcacggcgg tcaaaatcat aatccttttc gccgatgtaa tgatatttct
     4741 cgttgatgat tttcttggca tcggcgaatt ttatacgcgg aaaatcgggg atgtctttct
     4801 caatcttttt ttggtacaag cgttcatatt cgtcgccgat ttcatcggct agtttattca
     4861 ttaccgcctt gcacacagct tcttccatgt ccatcacgtc gtgatgggag tcgatccagc
     4921 tgatctcgca gtcaacacta gtgaactcgg tggcgtggta tgccgtatgg gagttttcag
     4981 cccggaatgc cggagcgatc tcgaaaacat tgttgaagcc ggatgccatc gccatctgtt
     5041 tgtaaaactg gggcgattga gccagataga cagtgcgtcc gaaatagtct agtttgaaaa
     5101 cttcggcccc cgattcggaa gcggttccca aaatcttcgg tgtgtggatc tcgatgaaat
     5161 tatggtttgt ccaatactcg cgcatcgcca tttcagcaat ggtctgggct ttgaaaatca
     5221 gatagttctt ttcgaggcgc aagtccagaa aacggttatc gagccgtaat tccttgttgg
     5281 atttgttggt ataatccaat atgtcaatcg gcaattcgtc ggctgccagg ctggtgatga
     5341 tgaaatcact gggaatgact tccattcccc gaagtttcac atgcggattt tccatcagaa
     5401 gacctttgac gcttacggtg ctttccttag tcagaccgga tacaacctga ttgattgcag
     5461 tattgtcctc gtttttctcg atcgtgatct ggatcttgtc ggtggaatct cgtaaaacta
     5521 cgaactggac atactgaaga tcgcggactt tgtccacgaa tccatcgatt ctcacctctt
     5581 cgccataatg ggaaggaaga tctttaaaat ataatttctg cattgttttc accatccgtt
     5641 gtgccttttt gtattatatc atgccaaccg gtcacaattc aacatcaaaa cggctgaaaa
     5701 ttgccttatt ctccgctttt tgcctgatgg acaacaacct gtgggaaagg aatctcgatg
     5761 ttgttttgat cgagcagttc cttgatgcgt tgtctggtct gtctttcgac tccgtaatgt
     5821 ttttcggtca ttgttttgac aatgaccctg atcacgacgg cgctgtcggc taggtcaatg
     5881 acgccaagca cctgcggcgc ttcgattatt tcttctattt cgtctttcag tttgggcagt
     5941 tccgccttca tcagagcgac ggtcttctcg atatcctcgc catatgcgat accaaaatcg
     6001 atgatcgcca gcgacggatt gcgggaatag ttgatgagat tagtaatatt gccattggca
     6061 attatcttca cttcgccttt ccagttcctg attctggttg ttttcaagcc gatctccgac
     6121 acttctcctt tgaaaccttc gatctccacg atatcgccga catcgaaatg gtgttcgaag
     6181 atgataaaga atccgctgat tagatcattg atgaaattct gggctccgag accgacaacc
     6241 aagccgacaa ttccaagtcc ggccagcgcc ggtacgacat tgacacccca cagggcaaga
     6301 atgaccaaaa caacgatgat gacgacagtg tatttgacaa tacttaaact gactttggcg
     6361 attgtcatcc ggcgtttctg taacggtccg gccttaagac cgacctttcc gaaagcatgc
     6421 ttgctgaagg atacaatggc aacagacagg aaaacagtga tgatcgatcc gattatcgcc
     6481 ccgatcttgt caagcaaaaa ggtggcggtg ttcgtaaaaa agtcgtcaaa taaggtggca
     6541 aaatcgaagt tccacaataa catcacgccg gcaagtgcgg caaaaaacac caccgcatca
     6601 gctaaatata tcagaatgaa ccagcccttg cgaaatgcat tttcgctttt ggaaaccagt
     6661 tttttctgcc aaagataagc caatatcagc gtggctgcga ttgctccgct caaaaaagcg
     6721 cgttcccagt cagcaagagc caaaaagaca gtcattgcga tcacctcaat tataattata
     6781 aaaaaattat aacatagaaa tattcagaat gccacatctt caccttgttt gattgattat
     6841 aaattataaa aaaaacaccc gtttatacgg gtgttacgac cagctggtcg agatgatggg
     6901 attcgaaccc acgacctctt agtcccgaac caagcgcgct accaaactgc gccacatctc
     6961 gtctggcgga agaggtggga ttcgaaccca cgtgcccttg cggacaactg catttcgagt
     7021 gcagctcgtt acagccactt cgatactctt cctgaaaaca aaaccctatg cttgtcatag
     7081 ggccaaaatt tctatggagc aggtaaaggg aatcgaaccc tcataatcag cttggaaggc
     7141 tgatgttcta ccattgaact atacctgcgt gacccggctt tggtcagatg ccggcgattt
     7201 cgttgttttg cgggaaatcg caaggatatg atatcacaaa gccaccttga ctgtcaataa
     7261 aaatatcgtc tgtcacaagg ttttctcgac ggctctcagt ttggcgctgt gagccctgtt
     7321 gttatcgttt atttcgtcat ccgacggttt caccacatgc cttgttacca gtttcaatac
     7381 cggtttctcg tcaggtatca ccggcagtcc cttcggtatc tcgatcgtgg atagacgttt
     7441 gaacatatcc ttgcagatgc gatcttctag agaatggaag gtaatgacga cgattcttcc
     7501 gcccggtttg agaatgtcaa cagaatcaaa caatgatttt tctagaaccc cgagttcgtc
     7561 gtttacaact attctcaacg cttggaaaac ctgtttggcg gggtgtcctt ttttatgtaa
     7621 gatagcctgt ggcagggcgc ttttgataat atccaccagt tcggacgtcg tttcgattgt
     7681 ttttttcata cggtattcga caatctttct ggcaatcggg cgggcaaaag ggtcttctcc
     7741 ataccgatag aaaatattcc cgagttcctt ttccggatag gtgttgacga tgatggatgc
     7801 ggtaattgga ttagtcttgt ccatccgcat gtccaaaggt ccttcctgac gataggagaa
     7861 tccgcggtcc ggcatgtcga actgatagct ggaaaccccg aggtcataca aaatcccgtc
     7921 aaccttttcg attccaagtg atgaaagttg cttacgacaa ttgacaaaat tgtcattgat
     7981 aaaaacgaca ttggcgtatc tggccaggtt tttcttagct tcgcctagag cgaattcgtc
     8041 ctgatcaaaa caaaaaagat gtcctgtgcc atcaagtttt tccaagattc tcttgctatg
     8101 gccgccgccg cccgtcgtca tatcgacata aatgccgtta tgtctcactt caagatattc
     8161 gattgcttct tccaacaaca ctggaacatg tcctgccaat ttgatcacct ttgttattat
     8221 atcataaaca aaaagaaaaa cgctgtattc cagcgcttct ttcttttatt tctcgtcttc
     8281 ggttttttcc ggttttggtt ctttcaccag gacacccttg tagtatccgc attttttgca
     8341 aacctgatga gccaaggtca gttcgccgca atttgggcat acgaccattg ccggagcgct
     8401 taacttgaaa tgagtccgtc ttttgcgttt taccgactta ctagtctttc gaaatggtac
     8461 agccatgttt tcacctcccg agccgtcagt tctttttttg gagttttgcg aagggattgt
     8521 cggccatttt ttccgcttcg tccagttcta tgatctcttc gcgataattc cgataggcat
     8581 tctcgctcaa aacccgcatt ggtttttcaa ccatgatctc tgcccagata aaaggatcga
     8641 gatcgatggt ggttccttcg atcggcaaag tgaaatcatc tttcacgaca gaggcaaact
     8701 ccaggatcga gtggaaattt agcggtacgt ccacttcatc caaagttatg gcacaaagca
     8761 tggttaaatg acaggcgatg tcgatagaaa agatataacg ttctccgatc tcgtcatatt
     8821 cgaaggtccc gctgacattc accggcgata cccccgccaa atcttcatca ttttcgccga
     8881 tcagatcgga acaatcacag acataggcga aacggttgtc tgtccgatga agtttgcgca
     8941 gttcaaaagt cgaccacttc ataccatcac ctcagcacag caaataaatt atacttgttt
     9001 tgacatggga tgtcaaggaa aaaacggtca gtaacggttg atctccgtaa agcaatgttg
     9061 atgcacagga ccgagcacaa agaagccctc gcccgaacat ctcaggcgtt tgaatcccat
     9121 ctctttttgc cgcagtttca gatacatcga cagagtgccg aattcctctt tgttcttgaa
     9181 ttggcttttg tgtaatttta aagaatcaag ttgattaaca aagtccgtct tcgtcgtttt
     9241 catgatccgg ttgggacggt tcgtgaaata ataagctaaa atgcgataac ggaaattacc
     9301 tgtgtttcca taagccacgc cattttggct agccatcaac ggaaacgagg aaaacaaaga
     9361 agccgtcccg gccgcttctc ccgttttaag atgatcggga tgaatctcgg aaggcatccg
     9421 cggatccgga cagaaaacaa catcgggatc gaaatcaatc aggacttcag ctatcttttt
     9481 cgctacattc catgcctggt attcaccgcc atccggaaat ccgagatgga caactttgtc
     9541 cacgccaagt ttctcggccg attgttttgc ctcttctttt cttgttgcta caagccggtc
     9601 tacggatatg ctcggatcgt cggttccgga gccgccgtca gtaacgatga caaacattat
     9661 ttccttccca agtcgcttaa gcattgaaac cgtcgatccg gctccgactt ctatatcgtc
     9721 cggatgcggt ccgacaaagc aaaagcgcga ggactttgta atatccggga tcggtacgaa
     9781 atgtttcatg atcttaatca gcattctttc accttcgttt cattcaaagg tattataaaa
     9841 caatttccga accgctgtaa aggcatcaag ccgtatccgg gtcgtttttc tggctttttc
     9901 catcaaatat tgtataataa gatatggttt gcaaggtata aggaggcatg actgtgaaat
     9961 ataccaagga agaaatatcg gctaggatcg gcgaactcac cgatcctagc cgtaacggta
    10021 gatcgcttgc ggaactcgga gccatcaaac atatcggatt cgacgaagat aaacaagggg
    10081 ttgtactgct gatagaagtt ggcaagaaag acaacgaaac aaccgcgccc attacaagac
    10141 agatcgctaa aatcattaag attgacatgg gatatccttc gcttgtggtt gagttcgaac
    10201 aggcaaggcc aaaagcgctc acgaagacgg tcaagatcat cggaatcgcc agtggaaaag
    10261 gcggagtggg aaaatccacc gtcaccgcca atctggctta cgcactgaca tcacttggaa
    10321 aaaaggtcgg aatcatcgat gctgacattt acggggcaaa catgcccaag atcatggaat
    10381 gcgttgacga aaacctgatg cccgcgccaa acgagaaact atatccgctg gaaatagaca
    10441 aaatcgaaat ggtttcgact gaatatttcg tagaaaaaga ccacgccctc atgtggcgcg
    10501 gaccgctcct aagcaaagta ttaaaactgt ttttcgaaga cactctttgg aatccagaat
    10561 tagattatct gttgatagat ctgccaccgg gaaccggaga tgtcatgatg gatatcaaaa
    10621 attttgttcc cgactgcaag atggttcttg tgaccactcc ccatccggac gcatcacacg
    10681 tggcgatcaa agccggattt gcggccataa gtttgaaaga ggatctgctt ggcgttttgg
    10741 agaacatgtc ctatttcgaa accgaaggca agcaatacaa catcttcggc gaaggcggag
    10801 gaaagttggt agccgaaaaa ctcggtgtgc cgctaatcgg aagaataccg atcggacagc
    10861 cggcgggaaa aaagcattcc gtatttggag agaccgaacc gatcggcctg atctatctcg
    10921 ggatagccaa aaaaattatg gatttgtacg aagacaacta ggtttatccc tggttgtttt
    10981 ttattccctg caatttctga acgtaactac ggtatttgtt ccgatatccc aaagcatcct
    11041 tgtaacgctt gtggttgtga agaatctcta tcaccctgtc tgtcacgacc ccggtgtagc
    11101 agatattttg caggttcatc agatacggca acacaaagtt cactagataa tctttatagg
    11161 caaggctatt cttgtctttt tccaattcga gacaccggat ataatctatc gttttttccg
    11221 ttttgtaaaa agctgttgca ttttccaact ccgcaattgt ttttttgtat tcgtcatcca
    11281 attttctttc ccggtaagat gctgcaagaa tataaagata atcggaataa aaacgggtat
    11341 tcctgtctgt gaattcccta aatcgttccg gtttgtcgtc acagatcgac aatacggtaa
    11401 gataatagtt gatgatccag ccagaaagaa acatcaaatc cttttcatca aaagccacct
    11461 tgtcaaaaga tccaccataa attagaaata tgttgcgaaa taccatgagg tcggctattc
    11521 tcgagagatt gtgcgtcttt atgaaataag acatcgccgc ctcataactg tccctagccg
    11581 gagaaaaaag ataaaggtgg ccataaataa tatacttgac aaaagcaatc attgctccgg
    11641 cttgttgatt gacacaagat attttttcca cggaatcaat gatggctttc gcctgttcga
    11701 aattgaagag aaggacattg tacatagcgg cataaattga aaatatggcc agtccatatt
    11761 cttccaaaga attcagatac ttgaaaatct cgatatatag cggctttgcc tcttctaatc
    11821 tttccgtcat cacgagataa ccaaatctgg ccaggacaac caaaattcca aatttgtatt
    11881 cggagatttt ttcataaagc cggcgatact tctccttttc gtccctgaca aaataatcaa
    11941 atgatttttc gagtatcgac accatgtctt ctggaaaacc gatcgtatcc tggggcagat
    12001 ccattttctc catgatgaga taaagatttt cgcggttgac tacgatggca ttgttttcca
    12061 gtttcgatat gtaagtgttc gaacagattc ccttagccaa agcctcctgt gtcattttga
    12121 gttccagtcg cctttttttg atcgaaactc cgataccacg ataataaaac tgatacatct
    12181 cttcttttct tttttcaact tcgttttgta acctgttttg catgattatc ctctcctctt
    12241 ccaggcaaaa caaaaaatta cgccttcaat aaacattgta attataaatc ttcatattgc
    12301 agttggcaag agtttttttt tgagaaatat ttttactgtt tctttatgac atttttgttt
    12361 tatgagaaag ataataaaaa cacccgcctt gttataagag cgggtgtttg cattctttta
    12421 tcaaaataca ttccttgcaa cgtggatttc tggccgtgca atgatatctt ccgaaaaaaa
    12481 ggaatcgatg atgcgccgtt atccattccg acggatcgta cagtttctca agtttggctt
    12541 cgactttatc cggatcatcc ttttccttgg aaaatcctaa ccgccatgac actcttttga
    12601 catgggtatc gacgggaaaa tgtggttcat gcatccattc cgccaaaaag actcctgcag
    12661 ttttacggcc gattcctggc aattcttcca gcaattcgcg atttttcgga atgattcctt
    12721 catatctggc aatcacttct tttgacatcg caatcagatt cttcgctttg ttccgatata
    12781 gtccgatggt cttgatatcg ttttgcattt cggtttccgt ggcttttgca taatcttcga
    12841 ttttttgata tttttgaaaa agcgaagtcg tcaaccggtt gaccgacaga tcggtcgctt
    12901 gttggctcag catgatcgat accaaaagtt caagctcatt tctgaaattc agttcgcagc
    12961 cggcgtcggg ataaagttcg cccattttgt catagacaaa acgggctttt gatctagcat
    13021 tcatttttta tcccatgaat cataaaattg tttcaaggct tcggaatttt ccgctttgta
    13081 tgtggtctcg acggcttttt tggctttttg ccttcttcgc aaaagaatac cgtccacata
    13141 actaagcgat gatttgccgg ctttgatcga atccagcacg gctttcttga tatcaattat
    13201 gtcaaatttg tcctcgtcca gccacttttg gattatttcg atctctagcg gagtgagctg
    13261 cttcttgaat tccgtttcca acagatcaac tatctcttcg gcattggtag catatttttt
    13321 tggtttgtcg atgatttcgt tttttatctc tttctgataa ttcgccatga tcgtctgaat
    13381 ggtgccgtct aagagaaaca cttcggtttc cttgccattt tttccttttt tcaattcgat
    13441 tgtcacgtat tggcgtttga tgaggttgtc taaaattccg agaaggtctt ctttttccag
    13501 gcttaagttt ttgcctattt tggcagggct caaataagta ttgcctttca acatctgccg
    13561 atatagttcg attatgacga atgcttccgt ttcggaaaga ccggcatcga gatagtttcg
    13621 caaaatcaat ttttcaaaat cgatgatgcc ttcatcaatc agacgcttta tcaatgtgat
    13681 gtccaaatag atcactcctt atcgatgatg ccggatgcca gtttatccaa agtttctttg
    13741 gcagcgtttt gttcggcttc ttttttcgat tttccttttc cacagcccat gatgatatcg
    13801 tcatccatat agaccctggc tacaaactcc cggtcatggg ctggtcccga ttcggataca
    13861 atcacataat tcaaagtgcg tttgtccgac tgaacaagtt cctgcagttt gcttttataa
    13921 tctgaatctt cccgatcgag gctttctttg atccgaggat atacaactat ttccaggact
    13981 ttctggactt cgtcaaatcc tttgtcgaga aaaatggcgc cgagaaaagc ttcgaaagca
    14041 tcggccagca aagctggttt ttctctgccg gagtttttct cctcgccttt tccgagtaaa
    14101 agatagtctc ctagagaaat gcttttcgca tattcaaaaa gacttccctc gcagacatcc
    14161 tgcgcacgtt tcttggtcag gacgccctca tcctcgtcca agtgttcaaa aagatacttg
    14221 cccactgcaa gatccagaac ggcgtcgccg atgaattcca gtctctcgtt acttctggtg
    14281 ttatgctcat tggcatagga agaatgggtc atggcgcgga tcagcaagcc gaaatcattg
    14341 aaacgtaatt ggaatctggc ttcgagttgt ctgattttga cttcattcat cttttgcttc
    14401 accagtcgtt tcctgaatgt atttttccac tttaggaaat acgccgttgc gaaccacggc
    14461 agcggcttgg cggatcgcat tggagaatcc gtaggctttg gacgctccct gcgccttgat
    14521 gaccggacgg taaaggccat aaatcaaagc gcctccgatt tcctccggag aaaccatctt
    14581 cttaaaatga gccagatttt ttctggcaat caaggcagca attttaccgg taaaattctc
    14641 catcagggcc attttcagaa atgttcccat gcctttggcc gtaccctcca tcgttttcat
    14701 gacgatgttc gcggtgaatc catcgcttat caagatatcg catggcggat cgaagatcga
    14761 ttttggttcg acattgccgt aaaaaggaaa cagattcgtc tccttgaaaa gcgaaaacac
    14821 ttcatggtcg atctcacggc cttttccgtc ctcggtccca
//
LOCUS       DAOPJL010000027        14343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_4427_length_14343_cov_7.801162, whole genome shotgun sequence.
ACCESSION   DAOPJL010000027 DAOPJL010000000
VERSION     DAOPJL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14343
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4427_length_14343_cov_7.801162"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..7181
                     /locus_tag="PLP02_04475"
     CDS             <1..7181
                     /locus_tag="PLP02_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42728.1"
                     /translation="SLFVDPASAIGSSHSIYGDIQAFDGTYSWETTLDPQSSSDDYIT
                     YTDYRENYCTAAATVAACIAAADGKIIRIDTAEELYWFSMDVSYEYIYQSDNPAENVK
                     LSPEKIAVLLSLDYVLGNNIDYSEVGAKMFVPVGYAFYDSENVWHQNLFTGTFDGQGF
                     VISNLYVAGYDYVTTTEMVDDEPVDLALSPYYTMFTINQGTICNLGLYDPTLELLDLH
                     INITKVSNLVGLNEGVVDHVFVYDDRTDVLEAGIRYKVGTSSEDFEAAGIVHTNSGTF
                     TNSYYVSKVVVNGSYINKFDSQPVLYANTGTIGNLVYDSDVYLLVVTVGTQTFTVATP
                     NAYASGETTAVLKSTSSTLNQTANHWYFYPNDSYPLAQGLEYDDTNDVFVISDAIDMC
                     VFSRLISFLSTYNGVTYGHSDYVLSNSIDMGTISPDAYMTPSATFYGSLSGYNASGSD
                     LGDNYYIFNLNITEGTIRGSAYYAGLFSILGSGSVVSDLNITQSEIALTDTEASYSKI
                     FYVGILSGRLSAGLIEDVQTDADIDLGTNAIGMTYAGGLVGQASGTINRVSVSGTMDF
                     NSHVYDSEYSVNPTFYIGGVIGGADAATLTAESLVNHSAIYGFGTASEFSLASGVSAI
                     QVAVGGVVGYIYNTTSVYHNFVSIANFGDIFLDSVINTVGLPSSQKAGGVFGLLSGLS
                     PVLESSGTYKFASFYNEGDINASYASGTSQIYAAGIGVSDSTEAVEYAMMYNHGAFSY
                     NTTGASYSDQLFKYTGTIYDIGSYSVTLSRVYNYSNFTYASNYYTEISPLYYSENDNA
                     TLIRYSANYGNVYFMTSSATTTINLASGLSVSGITTCDNVSLQNVSNTGNITLVNVNT
                     GSNTIHVSGMLCTLSDGDFIKNSLNKGNVVVADIAGSGNIFISGIVNTNDSGDLDDPE
                     QSTTQPIATEGVINSINYGSIKTSYDVSHYGINGTGNTFIGGIATLNTGSIQDSANLG
                     DIGAYNSSTSGTATFDTSTYYAGLVTGYTAGVVVGGIVGIVISGTSRIYDTANNGDII
                     AISYRFSRSGGILGVCLYDEADSGGITSGMGLTNDIEDSVLMNGLNFGDVSALTHIIG
                     VYSTTLYSETFAMRYGSGSSYLSGQSISISTVVGSSERPQINASAGGVIGYGLSVMRN
                     MLNHGTISSTDVAGGIVGATYVLGGAYSPYPVTYVDISTAINYGDIKAVPAANVMGSG
                     SNVDPYLLSIADLVTFYMADGNSFIYPTTYSDRFSPGTKRGFGGIFGRLQRGTNGTMS
                     SETGNFDFIVNANPNIDLIGRLDQVYNATSSTRYFRFNDAIYYSANINDTTQIVFTGF
                     YYAQYIVTSVTGDSRNWDVTATIYRYEQVGIVATRSNPDVVYGTYTYHSNRTAPSIGT
                     LLTSPAPYYIGGIEIPWITEDPYDANLTDPDTEYMYDPDFPMRTDPDLTEYIYYMEYN
                     LLADRFKTTGDNPRPYGMYVLSTSAGSTYGAVLPSNISLDDMKPIDEDYSPGISLFVD
                     YDNLSTIYTEDMPSEVVSAYNNLKQTIYNDKSEVIESETSEIVLTEASGGSDTVLDRA
                     SINYVSKRIDFTISLEAFLSAQSDVSYLITEIPTSAYALTAIRAYDYYGHTPSVSEME
                     AFNNLLSSEAGNGISISYPALLEVTLPSRTITTDVTLLLGYFAVYSEAFVGNDLFVGS
                     AYYTEYSVYVTFTPTVAYSGGSIGITGVAFNGGGTIDASSTPGDVRSLGNVNSSGSIS
                     FIFTDTAGILTDGYDFADNFVLKYYDSSVISDEYYSLSSTPTVVSYGTGTYEISFTFY
                     GNLRMGDYYLAYRYFPSSTEYTVYFDKSASSAKNIIGFTYYSEDDSLAINGLSITSDV
                     NLGYTLAIDTSANNFTVNTDTGLAAYLSNVTYDISYMSLGSFEISPFASVSSARLVGY
                     TYTNGYKDYEIEYVITAEDGSYSTYAHHIYERDIDFTAVLKNGNENDPGDVTAAREDE
                     LTTFSVDLGLDQSLDLYNLIVGSESYIAISLDATELDGLTPMDPAAIAGITYDASDYL
                     MIYMSYETLPGIYTFSFVMYRDGTANYVTLSTSLVISKLEGVDPYLSDIKFSQLANET
                     SYPDMSTANSTGQTNTDYYPVVYFAGIDYDGADAVYYEYFKIDGQVSNIPLEAYVPYM
                     LDYLPYGATISRYAYETGTGWYWTAEVSTGASAEEEAVLRTNYTVFPDTGLEPGETEE
                     VMILYRVTSEDGLHHAYYYITVTDIEFNTTFIFNVYYCTGATEDSCTLASQSVDFSDE
                     MVIITVKNILTNGDDTVTGVDDPDDYPTFTTISGMQSKMTQFIYTYDGDYRYSFGRNR
                     SGFFVFTVELPQDQYLNDLYTYEIKYDVYYLDNVSDYVSGIDGKYYYIGYSTRNRTRR
                     FNVYIRDKDPVSTDKPWGLFDFFRSWGE"
     gene            7203..9764
                     /locus_tag="PLP02_04480"
     CDS             7203..9764
                     /locus_tag="PLP02_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPF42729.1"
                     /translation="MAKRNKTNGNRYNSLARKIVGLTVFLNAIFLVLAVAVLSFIYIG
                     NLKSTRAEQLDTQVMNATGSFEAFVRDTAYDAETLSENQAVIAYLEYLNGDNEPVITD
                     PEDPNYLLYYDFSNLLETMSDFSYQGVYNFVFLATLDNSTIGPNGFFIGTGGVMADTS
                     WNLTERQWYLDLGSAEASVSKPYVDAISGEYAITFAHTVKQDGNTIGYVGIDVLLGSF
                     PDVCVAYDYYTEETSRALAVFSGTDDPLLIHFSETDYPDYNMLDYSQASLQDGTYGFT
                     GQGMAELIAGIGSSDPGVMELFGNDYLVGYSPIEGTDWTLAVMIETSPIPTLEATYLV
                     LLAATILLMVLVSFILSKNVRQTLSPIDDILESIEEIKKGNYNVHVNVEKTSELKQVA
                     EAINIMSREIGKQVELVYKSFVYDSVTGLKNRKASHQEIDDIMHQETEKVAVCLLEVD
                     NMKNINVTKGQAVSDEILRLIATDIRKYVTDTEALYFNGGSEFVLLIKNVRTLDRVET
                     EIGRIMELFKEPLDVNNLKIEVVFRAGISIYPSDGKTIVDLIKRCNVALFKARETASA
                     KIIFYNDKLTEEVNFKAQISEQLAMAIKNEQLYLKYQPLVDSKNEIYGFEALARWKSP
                     TLGEISPQIFVANAEESHLIIPIGNWILHQACLAQVEMKKRYGIKFVMSVNVSPIQLM
                     QKDFIDVLTRIVRDTEIDPHFLVLEITEGVLIESSIYVEELMNYVHEIGAKVALDDFG
                     TGYASLTYLKQYPFDNLKIDKSFVDGIFGAKKDHSIIATIVQMVHNLDMKVIAEGVEK
                     RKQYEYLKQIQTDVFQGFLFSKPLNFEEASRYVDQFYKVAKNKRIDVFAGRDYYE"
     gene            9777..11303
                     /locus_tag="PLP02_04485"
     CDS             9777..11303
                     /locus_tag="PLP02_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HPF42730.1"
                     /translation="MAYIFMHALRMLSENGTSQAFETVVSALIVLLTAVLLFLLFRNI
                     SKELRKSREDRKAIKKAHDEPKPISGAGKVATNVLFDKKSGGLLDQIDYWITHAAEGT
                     VAVLFYMNLDGFHYIAEKYSQKEIDKIIMELEKRLKKFAKKGSLCGHLRQDVFLYFMT
                     GDIDGEGIKQTGEDLLKLVNEPLKAVQEEITTSIGIVLFPYDGIDSAQLIKNAEVALY
                     VSKREGKNRYHLYSQDMIDKEQFNIDYYQEIKRSISNDEFILYYQPIVDIKTGRIIGL
                     ESLLRWNHPTMGVLPPSKFLNVMDLTGDIVWFGSWGFEKILKQYSTWKDQFRFRDIFI
                     STNLSPKQLMVEGLAKTFYNSVQKYELQTEMFCLEIIDYYTVIKNPIAMNNISEFRKY
                     GFRIASDDMGDNYELVSDMTKIRANIIKINRENTLKVMNDFEDIDKIVRVIKIALQEE
                     KVVIAEGIENEAMIRKMASFDIRFLQGFYFSTPKAAAEIGRMLENSPWNMDTFSEFTK
                     "
     gene            11452..12669
                     /locus_tag="PLP02_04490"
     CDS             11452..12669
                     /locus_tag="PLP02_04490"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008800834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPF42731.1"
                     /translation="MENKVFLVGAKRSPIGKYLGSLKDVHPVELGRKVLTELLEKTKV
                     PVGKVSEVIVGNILSAGLGQGIARQISVKSGIPESVPAYSLNMVCGSGMKAIMNGFSC
                     IRSGFDEIVVAGGVESMSQAPFLVSGKIRDGIKMGDFSLVDHMINDSLTDAFGNMHMG
                     ITAENVAEKYGIIREKQDEFSIASQQKAIEAVDSGRFADEIIPIEIKVKKETIVFDKD
                     EYPNRTSNMEKMAKLRPAFKEGGTVTAGNASGINDGASFTIIASEKAVKENNLPVMAE
                     IIAVGQGGVDPAYMGMGPVPAIENALKMAEMDLSEIGLIELNEAFAAQSLAVIGELSK
                     KYNVNEEKIMEITNVNGGAIALGHPVGASGNRIVVTLIHEMIKRDVEYGLASLCIGGG
                     MGTAIILKRNRKG"
     gene            12674..13393
                     /gene="fabG"
                     /locus_tag="PLP02_04495"
     CDS             12674..13393
                     /gene="fabG"
                     /locus_tag="PLP02_04495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPF42732.1"
                     /translation="MKLENKIAIVTGGARGIGGEIAKEFAKEGALVIAADMTELSYEM
                     ENVIPYILNVTDSAACKAFYDEIIARYGKVDILVNNAGITRDSMTRKMTDEMWDIVLD
                     VNLKGIFNLVRYFGPKMEEDGGGSIINISSVVGVYGNIGQANYAASKAGIIGLTMTWA
                     KEFARKGVPVRVNAIAPGYVLTDILKTVPQDLLDRFSQLTMLKRLGRPEEIAKVASFL
                     ASDDASYITGQTINVNGGMRL"
     regulatory      13521..13598
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            13762..>14343
                     /locus_tag="PLP02_04500"
     CDS             13762..>14343
                     /locus_tag="PLP02_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42733.1"
                     /translation="MSRKAKLAERIVYASKAARIAKTSRLGIFVSVIMTFLIFSISYY
                     GEVVGNFTFRVDKNSYEAGLSLVKDPDTQDYTSVLFADKVESADGMTCFCGTPYTLYD
                     IGDDVCLPTDEEVTSIDGSHNGESYLAYTFYLENAGIKIVDLQATINLLSTTRGADES
                     VRVRLFVNDEAETYAKVQSSRGTNPGEPEIETTP"
BASE COUNT         3818 a         3526 c         3449 g         3550 t
ORIGIN      
        1 tgagcctttt tgtcgatccc gcttctgcca tcggaagcag tcattcgatt tatggcgata
       61 tccaagcatt tgatggaacc tacagttggg aaaccactct cgatccccag agttcgagcg
      121 atgattacat aacatatacg gactatcgcg aaaattactg caccgcagcg gctaccgtgg
      181 cggcttgcat cgcagctgcc gacggcaaga tcatcaggat cgacacggcg gaagaactct
      241 attggttttc catggatgtg tcgtacgaat acatatacca atccgacaat ccggcggaga
      301 atgtcaaact ttccccggaa aaaatcgccg ttttgctttc gcttgactat gtgctcggca
      361 acaacatcga ttactccgaa gtcggagcca agatgttcgt tccggtcggg tatgcattct
      421 atgattcgga aaatgtctgg caccaaaacc tgtttacggg aacgttcgac ggacagggat
      481 tcgtcatatc caatttgtat gttgccggtt acgattacgt gacgacaacg gaaatggtcg
      541 atgatgaacc agttgatttg gccctttccc cgtattacac catgttcact atcaaccagg
      601 ggacgatttg caatctggga ctatacgatc cgaccctgga acttttggat ttgcatatca
      661 atatcaccaa ggtttccaat cttgtcggtt tgaatgaagg cgtcgtggac catgtttttg
      721 tctatgacga ccgtacagac gttctggaag ccggaatcag gtacaaagtc ggaacctcga
      781 gcgaagactt tgaagccgcc ggaatcgtcc atacgaattc cggcaccttc acaaattcct
      841 attacgtgtc caaggtggtc gtgaacggtt cttatatcaa caagttcgac agtcagccgg
      901 ttctttacgc caataccggc accatcggca atttggtcta tgattccgat gtatatcttt
      961 tggtggtcac ggtcggaaca cagactttca cggtggcgac tccgaacgcc tatgcttccg
     1021 gtgaaaccac agcggttttg aaatccacgt cctcgaccct gaaccaaacg gccaaccact
     1081 ggtattttta tccgaacgat tcctatccgc ttgcacaggg actcgaatac gatgacacaa
     1141 acgatgtgtt cgtgatttcc gatgcgatcg acatgtgtgt tttctcccga ctgatatcct
     1201 tcttgtcgac ttacaacgga gtgacctacg gccattccga ttatgttttg tccaacagta
     1261 tcgacatggg gaccatcagt ccggacgctt atatgacgcc atcggcgact ttttacggtt
     1321 cgctttccgg atacaatgcc tccggttcgg atttaggcga caactattat atattcaatc
     1381 tcaacatcac ggaaggaacg atcaggggca gcgcgtatta tgccggactc ttcagcattc
     1441 ttggctccgg tagtgttgtt tccgatctga acatcaccca atcggagatc gccctgaccg
     1501 ataccgaagc cagctattcc aagatattct atgttggtat tctttccggc aggctgtcag
     1561 ccggtttgat cgaagatgtc cagaccgacg ccgatatcga tcttggtacc aatgccatcg
     1621 gcatgaccta tgccggcggg ctcgtcggcc aggcttccgg tactatcaac agggtttcgg
     1681 tttccgggac catggacttc aattcgcatg tgtatgattc ggaatacagt gtcaatccga
     1741 ctttctatat cggcggtgtg atcggcggag ccgatgctgc caccctgact gcggaaagcc
     1801 ttgtgaacca ttcggcaatc tacggtttcg gcaccgcttc ggagttttcg ctcgcaagtg
     1861 gcgtttccgc gatccaggtc gccgtcggcg gcgtggttgg ttatatatac aatacgactt
     1921 ccgtttatca caatttcgta agcattgcca acttcggcga tatctttctt gatagcgtca
     1981 tcaacaccgt aggattgcct tcgagccaaa aagctggcgg agttttcggt ttgttatccg
     2041 gactttcccc ggtgcttgaa agttccggca catataaatt cgccagtttc tacaacgaag
     2101 gcgatatcaa tgcttcctat gcttccggca cgtctcagat atatgccgcc ggaatcggag
     2161 tctccgattc gacggaggcc gtggaatatg cgatgatgta caatcacgga gccttttcat
     2221 ataacactac aggagccagt tattccgatc agctgttcaa atataccgga actatctatg
     2281 atatcggatc gtactccgtg acgctttccc gcgtctacaa ttacagcaat ttcacttatg
     2341 cttccaacta ttatacggaa atttctccgc tctattattc ggaaaacgac aatgctaccc
     2401 tgataagata ttccgccaac tacgggaatg tctatttcat gacatcgtcc gcaacgacga
     2461 cgataaacct tgcatctggt ttgtcggtga gcgggattac gacttgcgac aacgtcagtt
     2521 tgcaaaatgt ttcgaacacc ggaaacatta cgcttgtcaa tgtcaataca ggatctaaca
     2581 ccatccatgt ctccggaatg ctttgcacgc tttcggacgg agatttcatc aagaattcgc
     2641 tcaacaaggg caacgtggtc gttgccgata tcgccggcag tggcaacatc ttcatttccg
     2701 gaatcgtgaa cactaacgat tccggtgatc ttgacgatcc ggaacaatcg acaactcagc
     2761 cgattgcgac ggaaggagta atcaattcga tcaattacgg aagcataaag acttcctatg
     2821 acgtgtccca ttatggtatc aacggaaccg gaaacacttt cataggtgga attgccaccc
     2881 tgaataccgg ttcgatccag gacagtgcca atcttggcga tatcggagca tacaattctt
     2941 cgacgagcgg tacggcgact tttgacacca gcacttatta tgccggtctt gtgaccggtt
     3001 atacggccgg tgtggtcgtg ggcggcatcg tcggtatcgt catttcggga acgtcccgga
     3061 tctacgatac cgccaacaac ggcgatatca tcgccatcag ctatcgtttt tcacgctctg
     3121 gcggcattct cggcgtttgt ctttatgacg aagccgattc cggcggaatt acttccggaa
     3181 tgggactgac taacgatatc gaggactcgg ttttgatgaa cggactcaat ttcggggatg
     3241 tttccgctct tacccatatc atcggtgtat attcgactac gctttattcg gaaacattcg
     3301 ccatgcgtta cggatcggga agttcttatc tgagcgggca gtcgatatcg atatcgacag
     3361 tcgtcggaag cagtgaacgt ccacagatca acgcttcggc ggggggcgtc atcggctatg
     3421 gtctttccgt catgagaaac atgttgaatc acggaacaat ctcttcgacc gacgtggccg
     3481 gcggcatcgt cggcgccacc tatgttctcg gcggagccta ttcgccttat ccggtgacat
     3541 atgtcgatat atccactgcc atcaactacg gcgatatcaa ggcggttccc gccgccaatg
     3601 tcatgggttc tggcagcaat gtcgatccat atttgttaag catcgccgat ttggtaacct
     3661 tttatatggc agacggcaat tcattcatct atccgactac ttactctgac cggttttctc
     3721 ccgggaccaa acgcggtttc ggcggtattt tcggccgttt acaacgcgga accaacggta
     3781 ccatgtcttc ggaaaccggc aatttcgact tcatcgtcaa cgccaatccg aacattgatt
     3841 tgatcggccg tctggaccag gtttacaacg ccaccagttc gaccagatat ttccgtttca
     3901 acgatgcgat ctattattcc gcaaatatta atgacacaac ccagattgtg tttaccggtt
     3961 tctattatgc ccagtatatc gtcacttcgg taacgggaga ttcccggaac tgggatgtta
     4021 ctgcgacgat atatcgttat gaacaggtgg gtattgtcgc caccagatcc aatccggatg
     4081 tcgtttacgg aacttacaca tatcactcca accggacagc tccgtcgatc ggaacgctgc
     4141 ttacaagtcc ggcaccgtat tatatcggcg ggatagaaat cccgtggatt accgaggatc
     4201 cttacgatgc caatctgacg gatcccgata cggaatacat gtacgatccg gattttccga
     4261 tgcggacgga tcccgatctg accgaataca tctattacat ggaatacaat ttgcttgcgg
     4321 accgtttcaa aacgaccggt gataatccga gaccatacgg aatgtatgtt ctttccacat
     4381 cggcaggatc aacctacggc gctgttttgc cgtcgaatat cagtctggac gatatgaagc
     4441 cgatcgatga ggattactct cccggcattt cgctatttgt cgactacgac aatctttcga
     4501 cgatctatac cgaggatatg ccctcggaag ttgtcagcgc ctacaataac ttgaagcaga
     4561 cgatctacaa tgacaaatcc gaagtgatcg aaagcgaaac atcggaaatc gtcctgacag
     4621 aagcgtccgg cggttcggac acggttttgg accgcgccag catcaattac gtgagcaagc
     4681 ggatcgattt caccatcagt ttggaagctt ttctttccgc ccaatccgac gtgtcttatc
     4741 tcatcaccga gattccgacg tcggcttacg ctctcacggc gatcagggct tacgattatt
     4801 acggacacac tccgtccgtt tccgaaatgg aagcatttaa caatctcttg tcttcggaag
     4861 ccggaaacgg aatatcaatt tcctatcccg cccttctgga agtgacgctg ccttccagga
     4921 caatcacaac cgatgtcacg ctcctccttg gttattttgc ggtttattcc gaagccttcg
     4981 tcggcaacga ccttttcgtc ggttccgcat attataccga atattcggta tatgtgacat
     5041 tcacgccaac ggttgcttac agcgggggat cgatcggcat caccggcgtc gctttcaacg
     5101 gcggagggac catcgacgct tcctcgacgc ctggcgatgt cagatcgctt ggaaatgtta
     5161 attcctccgg gtcaatcagt ttcattttta ccgataccgc gggaatttta acggatggtt
     5221 acgactttgc cgataatttt gtgttaaaat attatgatag ttctgtgatt tcagatgaat
     5281 attattcttt atcatcgaca ccaactgtgg tatcatatgg aacagggaca tatgaaattt
     5341 cctttacttt ttatggcaat cttcggatgg gcgattatta tctggcttac cggtatttcc
     5401 catcctcgac ggaatacacc gtttacttcg acaaatctgc gtcttcggcc aagaacatta
     5461 tcgggttcac ctattactcc gaagacgatt ctttggcgat aaacggtctt tcaatcactt
     5521 ccgacgtcaa tctcggatat accctggcga tcgatacgtc tgccaacaat ttcaccgtca
     5581 acaccgacac gggactggcg gcatatctgt cgaatgtcac ttacgatatc agctatatga
     5641 gccttggcag tttcgagata tcgcctttcg cttccgtatc gtccgcccgg ttggtcggat
     5701 acacctatac aaacggatac aaggattacg agatcgaata cgtgatcacg gcggaagatg
     5761 gaagctattc gacttacgcc catcatatct atgaacgaga catcgatttt accgccgtct
     5821 tgaaaaacgg caacgagaat gacccgggcg acgtgaccgc cgcccgcgag gacgagctca
     5881 cgactttctc cgtcgatctg ggtttggatc aaagccttga tctttacaat ctcattgtgg
     5941 gcagcgagtc ctatatcgcg atatcgcttg atgcgaccga actcgacggc cttaccccga
     6001 tggatccggc cgctatcgcc ggaatcacct atgatgcctc cgattacctg atgatctaca
     6061 tgagttacga gacgcttccc ggcatctaca ctttcagttt cgtgatgtat cgcgacggaa
     6121 cggcgaatta tgtgacattg tcaaccagct tggtcatctc aaaactggaa ggcgtcgatc
     6181 cgtatctgtc ggacatcaag ttctcccagc tggcgaacga gacgtcttat cccgacatgt
     6241 ccacggcgaa ttctaccggc cagaccaaca ccgattacta tccggtcgtt tattttgccg
     6301 gtatcgatta tgacggagcg gacgcggtct attacgaata tttcaagatc gacggccagg
     6361 tttccaacat tccgctggaa gcatatgttc catacatgct cgactatctg ccatacggag
     6421 ccacgatttc ccgctatgct tacgaaaccg gaaccggatg gtactggacc gctgaggtat
     6481 cgaccggagc cagcgccgag gaagaagcgg ttctaaggac gaattacacg gtgtttccgg
     6541 atacgggact agaaccggga gaaaccgaag aagtgatgat cctttaccgg gtcacatcgg
     6601 aagacggact gcatcacgct tattattaca tcaccgtcac cgacatcgaa ttcaatacca
     6661 ctttcatctt caatgtctat tattgcaccg gagcaacgga agattcctgc accttggcgt
     6721 cccaatctgt cgatttcagc gacgagatgg tgatcatcac cgtcaagaac atcctgacaa
     6781 acggcgatga cacggtgacg ggcgtcgacg atccggacga ctatccgacc ttcacgacca
     6841 tatccggcat gcaaagcaag atgacccagt tcatctatac ctacgacggc gactatcgct
     6901 acagcttcgg ccgcaaccgc agcggattct tcgttttcac cgtggaactg ccgcaagacc
     6961 agtatttgaa cgatttgtac acatacgaga taaaatacga tgtttattat cttgacaatg
     7021 tttccgacta tgtttcgggc atcgacggaa aatattatta tatcggttat tcgacgagaa
     7081 accggaccag aagattcaat gtctacataa gggacaagga tccggtgtcg accgacaaac
     7141 cttggggact gttcgatttc ttccgttcct ggggagaata aaacgggaaa ggagtgcgga
     7201 aagtggcgaa aagaaacaaa accaacggaa acagatacaa ctcgcttgcc agaaaaatcg
     7261 tcggtttgac ggtgtttctc aatgccattt ttctggtgct tgccgttgcc gttctctcat
     7321 tcatctatat cggaaacctg aaaagcacga gggccgaaca gttagacacc caggtgatga
     7381 acgccaccgg atcgttcgaa gcgtttgttc gcgatacggc atatgatgcc gagactcttt
     7441 ccgaaaatca agccgtcatc gcttatctgg aatatctaaa cggtgacaac gaaccggtga
     7501 tcaccgatcc ggaagatcct aattacctgc tttattacga tttttccaat ttactggaaa
     7561 caatgtccga tttcagctac cagggcgttt acaatttcgt ttttctggcg accttggaca
     7621 atagtactat cgggccaaac ggattcttta tcggtaccgg aggcgtcatg gccgatacca
     7681 gttggaatct gacggaaagg caatggtatc tggacctcgg atctgccgag gcttcggtgt
     7741 cgaagccgta tgtcgacgcc atttcgggcg aatatgccat cactttcgcc cacacagtca
     7801 aacaagacgg caacacgatc ggatatgtcg gcatcgatgt tctgcttggc agtttcccag
     7861 acgtctgcgt cgcttatgac tactataccg aagaaaccag ccgggcttta gcggtctttt
     7921 caggaactga cgacccgttg ctgatacatt tcagcgaaac agattatccc gactacaata
     7981 tgttggatta ttcccaggct tccctgcagg acggaaccta cggattcacg ggacagggaa
     8041 tggccgaatt aatcgccggg atcggttctt ccgatccggg agtgatggag ctgttcggca
     8101 atgattatct cgtcgggtat tcgcccatcg aaggaaccga ctggaccctg gcggtcatga
     8161 tcgaaacgtc gccgattccg actttggaag cgacgtatct ggtattgctt gcggccacca
     8221 tcttgctcat ggtcctggta tcgtttatcc tttccaagaa cgtgagacag accctgagcc
     8281 cgatcgacga tatcctggaa tcgatcgaag agataaaaaa aggcaactac aacgttcacg
     8341 tcaacgtgga aaaaacaagc gaactcaagc aggtggcgga agccatcaat atcatgagcc
     8401 gggaaatcgg caaacaggtc gaactcgttt acaagagttt cgtgtacgac agtgtgacgg
     8461 ggctcaagaa ccgcaaggcc agtcatcagg aaatcgacga catcatgcac caggaaacag
     8521 aaaaggtcgc tgtttgtctg ttagaagtcg acaacatgaa aaacatcaat gtaacgaaag
     8581 gccaggccgt ttcggacgaa atcctgagat tgattgcgac cgacatccgc aaatacgtga
     8641 ccgataccga agcgctttat ttcaacggcg gcagcgaatt cgttctcttg ataaagaatg
     8701 tccggacgct ggaccgcgtc gagaccgaaa tcggccgcat catggaactt ttcaaggaac
     8761 cgctcgatgt caacaatctc aagatcgaag tcgtcttccg ggccggtatc agcatttacc
     8821 catccgacgg gaaaacgatc gtcgatctga ttaaaaggtg caatgtggcc ctgttcaaag
     8881 ccagggaaac cgcatccgcc aaaatcattt tctataacga caaactgacg gaagaagtca
     8941 atttcaaagc ccagatctcc gaacagttgg cgatggcgat caaaaacgag caactgtatc
     9001 tcaaatacca gccgcttgtc gacagcaaga acgaaatcta cggtttcgaa gccctggcga
     9061 gatggaaatc accgacacta ggggaaatca gtccccagat tttcgtggcg aacgctgaag
     9121 aatcgcatct tatcataccg atcggaaact ggatattgca ccaggcttgt ctcgctcagg
     9181 tcgagatgaa gaagcgctac ggcatcaaat tcgtgatgag cgtcaacgtc agtccgatcc
     9241 agctcatgca gaaggacttt atcgatgttt tgacgagaat cgtccgcgat accgagatcg
     9301 acccgcattt cctggttctg gaaatcaccg aaggcgtatt gatcgaatca tcgatctatg
     9361 tcgaagaact catgaactat gtgcatgaga tcggcgccaa agtcgccctt gacgactttg
     9421 ggaccggcta cgcctcgctt acgtatctga aacagtatcc gttcgacaac ctcaagatcg
     9481 acaaatcgtt cgtcgacggg atattcggag ccaagaaaga tcacagcatc atcgcgacca
     9541 tcgtccagat ggttcacaac ctcgacatga aagtgatcgc cgagggtgtg gaaaagcgca
     9601 aacaatatga gtatcttaaa cagatccaaa ccgatgtttt ccaagggttc ctgttttcca
     9661 aacccctgaa tttcgaagaa gcttcccggt atgtcgatca attctacaaa gtagccaaga
     9721 acaaacgcat cgacgttttc gccgggcgcg attattacga gtaaggagtg atatcattgg
     9781 catacatttt catgcacgct ttgcgtatgc tgagtgaaaa cggtacgtcg caagctttcg
     9841 aaaccgtcgt atcggccctg atagtccttt tgaccgctgt cctgcttttc ctgctattcc
     9901 gcaatatctc aaaagagctt cgcaagtcgc gggaagacag aaaagcgatc aaaaaagcgc
     9961 atgacgaacc caaaccgatt tccggcgccg gcaaagtcgc gaccaatgtt cttttcgata
    10021 aaaaatccgg cggacttctt gaccagatcg actactggat cacccatgca gccgaaggca
    10081 cggtagccgt gcttttctac atgaatttgg acggattcca ctatattgcc gaaaagtaca
    10141 gccaaaagga aatcgacaag atcattatgg aactggaaaa acgcctgaag aaatttgcca
    10201 agaaaggatc gctttgcgga cacttgagac aggacgtctt tctttacttc atgaccggag
    10261 atatcgacgg cgagggcatc aaacagacag gagaagatct gttgaaactg gtgaacgagc
    10321 ctttgaaagc ggtccaggaa gaaattacca cttcgatcgg tatcgtgctg ttcccgtatg
    10381 acggcatcga ttcggcccaa ctcatcaaga atgccgaagt agccttgtat gtttccaaac
    10441 gtgaaggcaa gaaccgttat catctttaca gccaggacat gatcgacaaa gaacagttca
    10501 acatcgatta ctatcaggaa atcaaacgtt cgatctcgaa tgacgaattc atcctttact
    10561 atcagccgat cgttgacatc aaaaccggga gaatcatcgg gttggaatcg cttctcagat
    10621 ggaatcatcc cacgatgggg gtcctgcctc cttccaaatt cctgaatgtc atggatctga
    10681 ccggcgatat cgtctggttt ggttcctggg gcttcgagaa gattctcaag cagtattcga
    10741 cctggaaaga ccagttccgg ttccgtgata ttttcatatc cacgaacctt tcacccaaac
    10801 aactgatggt cgagggactt gccaagacat tctacaattc cgtccagaaa tatgagcttc
    10861 agaccgaaat gttctgcctg gagatcatcg attactatac tgtaatcaag aacccgatcg
    10921 caatgaacaa tatttccgaa ttccgcaaat acggattccg gatcgcaagc gatgacatgg
    10981 gcgacaatta cgaactagtc agcgacatga ccaagatcag ggcgaacatc atcaaaatta
    11041 accgggagaa cactctaaag gtcatgaatg atttcgaaga tatcgacaag atcgtcagag
    11101 tgatcaagat cgccctgcag gaggaaaaag tggtcatcgc cgaaggcatc gagaacgaag
    11161 cgatgatccg gaaaatggct tctttcgata tccggtttct ccagggtttc tatttcagca
    11221 ctccgaaagc ggcggcggaa atcggccgca tgctcgagaa ttccccctgg aacatggaca
    11281 cgttctcaga atttaccaaa taaaagaacc ggcgaggaaa atcgccggtt ttcttttcat
    11341 tcgccaatct gtgaaagcgc ttgacaaaaa gcgaaggttt ccgctaaact atgtattggc
    11401 gttctcggat tccaatcggg aatcctttat cgaagacgga ggatgattaa catggaaaac
    11461 aaagtttttt tagttggagc caaacggagt ccgatcggca aataccttgg ttcgctcaag
    11521 gatgtccatc cggtcgagct gggcagaaaa gttttgacgg aactgcttga aaagacgaag
    11581 gttcccgttg gaaaggtaag cgaagtgatc gtcggcaaca ttctgtcggc cgggctcggt
    11641 caaggaatcg cccgccagat ctcggtaaaa tcgggaatac cggaaagcgt accggcttat
    11701 tctctaaaca tggtttgcgg atcgggaatg aaagcgatca tgaacggatt ttcctgcatc
    11761 cgttccggtt tcgacgaaat cgtcgtggcc ggaggcgttg aatcaatgag ccaggcgcct
    11821 ttcttagttt ctggaaagat acgtgacggc atcaagatgg gggatttttc ccttgttgac
    11881 catatgatca acgattcgct gacggatgct ttcgggaaca tgcatatggg gataacggcg
    11941 gaaaatgtcg ccgaaaaata tggcatcatt cgggaaaagc aggatgaatt ttccattgct
    12001 tcccaacaaa aggcgatcga agcggtcgat tccggaagat ttgccgatga aatcatcccg
    12061 atcgagataa aagtaaaaaa ggaaacgatc gttttcgata aggatgagta tccgaatcga
    12121 acgtcaaata tggaaaaaat ggccaaactc cggcctgcgt tcaaggaggg aggcaccgtg
    12181 acggccggaa atgcatccgg cattaacgac ggagccagtt ttacgatcat cgcctcggaa
    12241 aaggcggtca aagagaacaa tcttccggtc atggcggaaa ttatcgccgt cggccagggc
    12301 ggagtcgatc ccgcttacat gggaatgggt ccggttcctg ctatcgagaa cgctctcaaa
    12361 atggccgaaa tggatttgtc agagatcggg ctgatcgaac tgaacgaagc tttcgccgcc
    12421 cagtctttgg cggtcatagg cgaattgtca aaaaaatata acgtgaatga agaaaagatc
    12481 atggaaataa ccaatgtcaa cggcggagcc atcgcattag gacatccggt cggcgcttcc
    12541 gggaaccgga ttgtcgtcac tctgatccat gaaatgatca aacgggatgt cgaatacggt
    12601 ctggcctccc tctgcatcgg cggcggtatg ggcacggcga taattctcaa aagaaacagg
    12661 aagggatgaa accatgaaac tggaaaacaa gatcgcgata gtgaccggcg gtgccagagg
    12721 catcggcggc gagatagcca aagaattcgc caaagaaggc gcgcttgtca ttgcggcgga
    12781 catgacggaa ctgtcatacg aaatggagaa tgtcatacca tatatattga acgtgacgga
    12841 cagcgctgcc tgcaaagcgt tttacgacga aatcatcgcc agatacggta aagtggacat
    12901 tctggtgaat aatgccggta tcaccaggga ctccatgaca agaaaaatga cggatgaaat
    12961 gtgggacatc gtccttgacg tcaacctgaa gggaatcttt aatcttgtcc gttatttcgg
    13021 cccgaaaatg gaagaagacg gcggcggctc gatcatcaac atatcgtctg tcgtcggcgt
    13081 ttacggaaac atcggacaag cgaactatgc ggcatccaaa gccggaatca tcggactgac
    13141 gatgacctgg gccaaggaat tcgcccgcaa aggtgttccg gtccgcgtca acgcgatcgc
    13201 tcccggatac gttctcaccg acattctgaa aacagttccc caggatcttt tggaccggtt
    13261 ttcacaattg acgatgctga aaagattggg ccgtccggaa gaaatcgcca aggttgcgtc
    13321 gtttttagct tcggacgatg cttcatatat caccggccaa acgataaatg tcaatggcgg
    13381 aatgcgtttg taagcgtttt aaacattttt ttatcatgcc ttccaccgcc aaatatggcg
    13441 gtggaatggc ttttgtgaag aaaaagtgaa aaaaaataga taaaagtgct tgacaaagaa
    13501 atttgactat aatataatga ccgtgtaaat agcaaaacta acgaaagtta gtgacgcaaa
    13561 gctatagggc cttcgaaaga tggtagccag ttgccgttat cttttaaaga tgagggcgct
    13621 tttttatttc ttgggacaca tgtctattgt gtctttaacc aatgaaagaa aaaaaagcct
    13681 gaaatccgaa aaggatggtg gcttttttat tttagatgga gtataatcta aaacaatgat
    13741 gggggggatt accttgcgat catgagccga aaagctaaat tggcagaacg cattgtctat
    13801 gcttccaaag ccgcaagaat cgccaaaacc agtcgtttgg ggatattcgt gagtgttatc
    13861 atgacctttc tcatcttttc catctcttat tacggcgaag tcgtcgggaa tttcactttc
    13921 cgtgtcgaca agaactctta cgaagccgga ctttcgttgg taaaggatcc ggatacccaa
    13981 gattacacat cggtattgtt tgcggataag gtagaaagcg ccgacggaat gacatgtttt
    14041 tgtggaacac cttacacact ttacgatatt ggtgatgatg tatgtcttcc aaccgacgaa
    14101 gaagtaacct caattgatgg atctcataac ggcgaatctt acttagctta taccttctac
    14161 ttggaaaatg ccggtatcaa gatcgtcgat ttgcaagcga ccatcaatct cttgtcgact
    14221 acaagaggag cggacgaatc agtccgagta agactatttg tcaatgacga agctgagaca
    14281 tatgccaaag tccagagttc aagaggaact aatccgggag aaccggaaat agagaccaca
    14341 ccg
//
LOCUS       DAOPJL010000028        14121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_4524_length_14121_cov_7.952367, whole genome shotgun sequence.
ACCESSION   DAOPJL010000028 DAOPJL010000000
VERSION     DAOPJL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4524_length_14121_cov_7.952367"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLP02_04505"
     CDS             <1..243
                     /locus_tag="PLP02_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42734.1"
                     /translation="TKAVFCFAIASEYRKKGIATGLLKKVCEDAIKEGYDIVEAYPKK
                     EMKNLDNFEGPLSMYLKQGFSITEDHGEYYTVKKDL"
     gene            271..849
                     /locus_tag="PLP02_04510"
     CDS             271..849
                     /locus_tag="PLP02_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPF42735.1"
                     /translation="MRTDTENKEKLIDIIKQNVEVDKVINTLEKIDLPGWYVGAGCVV
                     QSVWNHISGFDPMYGIKDIDVIYYDEHDLSYEKEDEIRKMVKKECGYINLDVDVINEA
                     RVHLWYKQKFGIDLKQYTSTEDAIDSWPSTASAIGIRKAGTEYVIYAPYGLEDAFSGI
                     IRANKKVISKEIFLAKADKWKKKWEWLTILPW"
     gene            1018..1899
                     /locus_tag="PLP02_04515"
     CDS             1018..1899
                     /locus_tag="PLP02_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPF42736.1"
                     /translation="MSIDKITAVKQIQEYIQDNIKNPITLSDVSKQIHYSLWYSARIF
                     KEVIGINMFEYIRRLRLTEAAKTLRDTNNNVIDVAFDFLFDSHEGFTRAFSREFNITP
                     KAYQKSRIPIQYFIPYKVIAKSQGKEEKQVETKAVFIQIIERPKRKAIIKRAKTATHY
                     FQYCEEVGCDIWGMLCSIKEAISEPVGMWLSKKMVKPDTSIYVQGVEVPEDYSKEIPD
                     GYEIIELPETLMMIFQGEPYEDDNFEEEVTKVMDYVSRYHPEVYGYVYDIDGYRFQYA
                     PQGYRGYIEGRTVKKIK"
     gene            2039..2461
                     /locus_tag="PLP02_04520"
     CDS             2039..2461
                     /locus_tag="PLP02_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF42737.1"
                     /translation="MYRLQDLMMMFDVPERTIRRHIRIGILNGVKIGGTWRFTEDDIK
                     NYLSNSYIRESTRKRFLERVNEFLYGLSANKSDIFVSVNKHLDDQAQIERLTNFVNTF
                     ENTFFFDASSAGKDYVISFIGNKKDAIRLLDYLGDEND"
     gene            2454..>2972
                     /locus_tag="PLP02_04525"
     CDS             2454..>2972
                     /locus_tag="PLP02_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42738.1"
                     /translation="MTDLYQTLSDKIKQVDLDKIYKGFKSYPFALYDAKTIYLENETI
                     PWNEAFIGNTAITYEGKTMAIWDLRYQIEDTDIFASKLIHEMFHVHQLKNSESRFPNE
                     LKGLDYKYDELGMSLKILETICLQKSIQKNEKEEFNRFLAYRRHRESLYPENLDYESK
                     IEVVEGTARYVEL"
     assembly_gap    2973..3072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3073..3321
                     /locus_tag="PLP02_04530"
     CDS             <3073..3321
                     /locus_tag="PLP02_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42739.1"
                     /translation="FVEEYNRRIEANIAKILEAKCQKTIGEQITGLDPMNTYKIGIRL
                     YCKNFIRIRKEERDLFIFGESVVEQLPCSVIVYQKETP"
     gene            3572..6820
                     /locus_tag="PLP02_04535"
     CDS             3572..6820
                     /locus_tag="PLP02_04535"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPF42740.1"
                     /translation="MKRYCWLIMFLFVLFMTSCKAAEIQSSVTYSELMYETGETITLS
                     DTDLTVMVDNETITDFTVNILGTNIYYDAGDYQVEIEIILPDETSITTVVTVTIVDYP
                     LSDILMILGIGEHIHIIGEDDVDLMDGVSAIDRTYGDLTANIIVDDSDVDYETIGVYV
                     IYYTVQNADGYSRRETSTVTVRLFDVPETFPEEFAIVYYGGLYGVVDIDDNELIPCDY
                     NDISYLEEGIIELEKAGSLFYFNLYTGAFIEFDGMPNGFTEGRSVFVLDGRYGILSID
                     NEIIAPNIYSFAKLRDIEGYAQVRSGDYYGYVDSSGEEVIEPRFISMTDLLPKNDFEN
                     TLFLVRFIDLSYGIIDSNNDVAYYLTNSTILKYEIDENTYYGIYTADGLITVDDSFAK
                     ETFSIETDSLQIVEIDDSGLMIVCDSNGSYYLLDKNKNTLLSDIPFASFADLSYDESK
                     EVYVFLNNENVYVYDTSFVLVKEYDNSLDLNPADITNIDTENLRYQITGNHTFQVLYD
                     SSGNTVSLVRYDDIYIYDDFVVLYDELAFDIVFVDRQFSYIYDEIEGTGIFEPDYFVV
                     NNAASTTASIYSDTGALIKSGLNLVIENILKAQHGIYIINQGETNVYLYDQVEDNLVL
                     IDTVQGYLNFINGIYYSFYCESQTVLYSYAGGVLTKINAYDIAQGGMPEAIDLGYGYY
                     VVPSSDDLYYVTTPAIDTEYDIWIFPLSEDFYLEGSSNQISLKNRNTITLDTYNFTNS
                     YDDYPGSFTNIVKIEISPTEYLVRLQSLLGYYYWFFLSDTSIASVDLTEFQEDDRIAF
                     VNTDNDYWYIYSYNQSTLIYELSDQATFFSYIEYEDLYLITDGNTHIVRINGDDEFEY
                     IYTTSNSIVDLHTHADYLAVQEETDKVILYENSLGQTEKIMTIVDTLATFEINGEILL
                     GDHVISFDGNTYLIDGVTKDYPSIYFDSSTDILYGIDEDGIDAYFAGSCVMTANTNVM
                     SLTSGFYYCYESGNISLYDGTDLLFTIDADEIEYIEDADSFMVKNDGTYGIIDMTGDY
                     VISNDYISIAFNDTYYIVTGSCYYGVYDENGNELIDTHYDDLWIYIVLEEQD"
     gene            6851..7363
                     /locus_tag="PLP02_04540"
     CDS             6851..7363
                     /locus_tag="PLP02_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPF42741.1"
                     /translation="MMNIEEAIFARRSIRTFKQDEVSDEAILKLLKAAMAAPSACNRK
                     PWLFYVVKNKEKQMQLRQMHRYSDYDSPLIIIVCADMRRKLGVKSEDFWIQDCSASVE
                     NLMLEAVGLGMGTCWCGLHPIEAAKKRVVKILNMPEHVVPVALIHVGHPAEHPTPRTQ
                     YDESLVTVIE"
     gene            7391..7969
                     /locus_tag="PLP02_04545"
     CDS             7391..7969
                     /locus_tag="PLP02_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPF42742.1"
                     /translation="MKKILLVDYGSTYFKDVILSFEQLGVECVKLRYDEITGHLDKNN
                     LCGIVLTGSPGRVNNPEDPQLDPALLETGVPILGICYGLQLLMHSLGGKVEALDKRDL
                     GFGLMTIIKESPINKGLGNPTNVWMAHYDHVTKLAKGFEIYASTPISIAMVGDEKRKI
                     YGIQYHPEAKRSDDDMKLLRNFTYDICGYTNM"
     gene            8008..8733
                     /locus_tag="PLP02_04550"
     CDS             8008..8733
                     /locus_tag="PLP02_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42743.1"
                     /translation="MLRIEQLTKSYQSGEKRNIILEKLDFSVIQNDFVVIMGKSGSGK
                     TTLLYCLGLIDDIDSGTISLNGKRIDNLGENEKAKIRLNEFGFVFQDMNLINMLTLKE
                     NIAMPSYEQKTYSSENVDKLIDSFGLTDFADKKPDRVSGGEKQRAAIARAIINHPKIL
                     FADEPTGSLDYQNSLSLLNLFKKMNNEGQTLVIVSHDRLVASYAKKIYFLQEKKIRQI
                     LEFDQETEEERFSAIDKALLGLD"
     gene            8869..11169
                     /locus_tag="PLP02_04555"
     CDS             8869..11169
                     /locus_tag="PLP02_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF42744.1"
                     /translation="MKKYNASQTEIVLDKYYDLDMHISDFSMETETFVATYPGISYYT
                     ARLTSKRHVVRYEEYVDNDYNNYRVLEGKPLDELELYEVAVPAGYNYYLDSTNEDGLN
                     IGDEITGYSHPLIVASIVDYIQYVEPAFNELTMQETAGDMSFAFEYMRFLASPDTISY
                     INSQLTDIQYQSIIYKIIFDDYSKEAEKEFVSAYLDYFDGGNSYIHVNPFYEVLDSSN
                     GMDRVIGKTVLIAALTLLCFGLIAIFVDIRRDLNRDRRQIGIEKATGISNNTIIRYYL
                     WKFGLAIGFGVLLAMMVFLIIAIILKNNYFYSFFLSKNFLSYLEGFAIILGIFGIALL
                     LMNLALKRILTKTPATFLIQSNSSERVKANRLSERNLPARLGFRFFMANPVSSMVQVL
                     AGSLVVIVTAVFLLFSGFAKNVYTPANYGLDYDYMIIAKYSNYSYIMDNYGESSLITA
                     SKIVWMQVGDENGDPVYVKAMYVAYSGDLCDFLASDIDEGTCPIDSSERGLGVVGYAL
                     AEDYHLKVADDEADSLSNYIWFTEQSLASTDTYNIKIYGIQTILQYDGYVVFDRIGTS
                     AVYSGRKFLDFYEANTTAAQVYINLEAGADKDALKTYLENNNIFYVSGEASQASIDDS
                     SAKLIFYLSGITVVGAVILIMLFSLVVIIDTSDFFYRRQKDFGILRSLGQSRKRIISM
                     NMFRQGIAATLFFVIGVATAFCLSNLIRRSFERTMGIYHLETGMWTIAPLVVGILIFV
                     LFLLNAFLLSLFYSKGPVADLVRHND"
     gene            11372..13192
                     /locus_tag="PLP02_04560"
     CDS             11372..13192
                     /locus_tag="PLP02_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42745.1"
                     /translation="MVKYNIFQNSKYVYEASFQKCPRLKGLLAINFLSELLLPFSATL
                     VTTVVVYALTNQLPAGEYIWIVFGLSLFTFILTGLEYWSYTTYTFENIFTRNSTFMIR
                     LTDHQLSTDYMNVEPKSRRDIISKAFEAIGSNMYGVELMMRSAPTALYNFVGMLIYGV
                     LIVIYSPVIVAILFVMTVVNIFLTSRANKYLASQRDRIGKIWDERYYLSQDATNHKYG
                     KDIRVYQMRNWFSRIFVALTKERTLIGKKVEKRFLFASLSNTIFLFIRDLVAYVTLAI
                     AVLNGSIDVTTFTFLIGIVTGFTVWLNGFVSHFNDLRKANIATNEYRECLQTKNEFNH
                     GEGVDTGKMSKPISIEFKDVEFSYPDSDNMTLKNISFKIAAGEKIAIVGDNGAGKTTI
                     VKLLCGLYKPTKGDILIDGVSIDKLNLEEYMRLLSVVFQDSEPLAFTIEQNIACDVPE
                     NIDHDRVQKSLEEADLIKKVESLPLKEKTYITEEFNKDGVKLSGGEIQKLMLARALYK
                     HAPILILDEPTAALDPISEEKMYLRYEDFLQGNTSVFISHRLSSTKFCDRILFIKDGK
                     VIEEGSHYDLMAKEGEYRDLFQIQAKYYQEGKDHDTENQS"
     gene            13170..>14121
                     /locus_tag="PLP02_04565"
     CDS             13170..>14121
                     /locus_tag="PLP02_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42746.1"
                     /translation="MTQKTSLKGNIKKMFHVMNVVKRVSKGYFYSFLADSFFRSIRPY
                     VTIIYTYLILDGLIDKIPQSQLLTYVFWMVGLNVVLEIILKILNYSKAKYLIELDFNL
                     ENQIAIKTYELDFCQIEDSSNMKLIQQAKEGSNGNGGLRLYCEYVMTGMIAAVLSIAY
                     GVILLSGMLKKATLVDTSGIAQFLNSPYSLVIIFVSMLIPGIISWFMMKLDNKKSYDV
                     MMGNIEGNRRFSYYFRIASNYKYGKDIRIFGIKDMILNEMKDKKNSVDANWRGYVNFA
                     VRIMSVTFFGNMLLSFIAYAMVGLKAIYGIITIGSVVSYVA"
BASE COUNT         4337 a         2697 c         3110 g         3877 t
ORIGIN      
        1 acaaaagcgg ttttctgttt cgctatcgca tcggaatacc gaaagaaagg gattgcgacc
       61 gggttgctga aaaaggtttg cgaagatgcc ataaaagaag gatacgacat tgtagaggca
      121 tatccgaaaa aggaaatgaa aaacctggat aatttcgaag gtcctttgtc gatgtatcta
      181 aaacagggat tttcgatcac agaggatcat ggagagtatt acactgtgaa aaaagatttg
      241 tgatgcggag aggaaaaaga gaataataca atgagaacgg ataccgagaa caaggaaaaa
      301 ctgattgata taatcaagca aaatgtggaa gtggacaagg taataaacac tctggaaaaa
      361 atagatctgc ccggatggta tgtcggagct ggatgcgtag tgcaaagcgt ttggaaccat
      421 atttccggat ttgatccgat gtacggaatc aaggacatcg atgtcattta ttacgatgaa
      481 catgatctgt catatgaaaa agaagacgag atcaggaaaa tggtcaaaaa ggaatgcgga
      541 tatatcaatt tggacgtcga tgtgatcaac gaagccagag tgcatctttg gtataaacaa
      601 aaattcggta tcgatctgaa acaatacaca agcaccgagg atgccatcga ttcatggcct
      661 tccaccgcat cggccatcgg aataaggaaa gccggaacag aatatgtcat ctatgctcct
      721 tatggattag aagatgcttt ttccggtatc atcagggcaa acaagaaagt aataagcaaa
      781 gagatttttc ttgcgaaagc cgacaagtgg aagaaaaaat gggaatggct tacgatattg
      841 ccatggtaaa cagaataaat gagattatat cggataaggc cggccgcaag ccggcctttc
      901 ttaatccggt tgttaacaat atcatcgcat ctttttttga tataataaaa gaatgacaac
      961 agaagtcaag aaatcttaaa tcgtatggga tacaatatga ttgtcgaggt gacttttttg
     1021 agcattgata aaataaccgc cgtcaaacag attcaggaat atatacaaga caacatcaag
     1081 aacccgatca cgctttcgga cgtatcgaaa caaatccatt attccttgtg gtacagcgcg
     1141 cgcattttca aggaagtgat tggaataaac atgttcgaat atatccgaag actgagactg
     1201 accgaggcgg ccaaaacgtt gagagacaca aacaacaatg tcatcgatgt tgcttttgac
     1261 tttttgtttg attcccatga aggatttacc agagcttttt cccgggaatt caacatcaca
     1321 ccgaaagcat atcaaaagtc aaggattccg atacaatact ttattcccta taaagtaatc
     1381 gcaaaatcac aaggaaagga agaaaaacaa gtggaaacca aagcagtatt cattcagatt
     1441 atcgaaaggc cgaagcgcaa ggccattata aagcgcgcaa aaacagccac ccattatttt
     1501 caatattgcg aagaagtggg ctgtgacatc tggggtatgc tatgcagcat caaggaagca
     1561 atctcggaac ctgtcggaat gtggctgagt aaaaagatgg taaagccgga tacatcgatt
     1621 tatgttcagg gagtcgaggt ccccgaagat tattctaaag aaatacccga tggatacgaa
     1681 atcatcgaat tgccggaaac cctgatgatg atcttccaag gcgaaccgta cgaagacgac
     1741 aatttcgaag aagaggtcac caaggtaatg gactatgttt ccagatatca tccggaagta
     1801 tacggatacg tttacgacat agacggatac cgcttccaat acgcgccgca gggctaccgg
     1861 ggatatatcg aaggcagaac ggtaaagaaa atcaaataac cgttatcgtg aagatatgcc
     1921 ggttctccgg catatctttt ttatgtctgc ttgtccatat tcaaacatgc caaaacatgc
     1981 aataatgtca ttgttttatt ttcccggttg ggttatcatg aaagctgggg tgatgataat
     2041 gtatcgacta caagatttga tgatgatgtt tgatgttccg gaaagaacaa tcagaaggca
     2101 tataaggatc ggaatcttga atggtgtgaa aattggcgga acatggcgat ttaccgagga
     2161 tgatatcaaa aactatctca gtaattcata tatcagggaa tcgacaagaa aaagatttct
     2221 cgagcgagtg aacgaattct tatatgggct ttcggccaat aaaagcgata tctttgtcag
     2281 tgtaaacaaa caccttgacg atcaggcgca aattgaacga ttgacaaact ttgtcaatac
     2341 gtttgaaaac acgtttttct ttgacgctag cagcgcagga aaagattatg tgatatcctt
     2401 catcggaaac aaaaaagatg caataaggtt attggattat ctgggtgatg aaaatgactg
     2461 acttatacca aacgttaagt gacaagataa aacaagtcga tctcgacaaa atttacaagg
     2521 gattcaaatc ataccctttt gccttatacg acgccaagac tatatatttg gaaaatgaga
     2581 ctatcccttg gaacgaagct tttatcggga atacggcgat aacctacgaa ggtaaaacca
     2641 tggccatctg ggatcttcgt taccaaatag aggatacgga tattttcgca agcaaattaa
     2701 tccatgagat gttccatgtc catcaactaa aaaattccga gtcgcgcttt cctaacgaac
     2761 tgaaaggctt ggattacaaa tacgacgaac ttggtatgtc tttgaaaatc ctggagacga
     2821 tttgcttaca aaaatcgatc cagaaaaacg aaaaggaaga attcaaccgg tttcttgcat
     2881 ataggagaca tcgggaatcg ctttacccgg aaaacctgga ttacgaaagt aaaatcgaag
     2941 tcgtcgaagg aaccgcccgt tatgtggaat tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnttcgtcga ggaatataac cgtagaatcg aagccaatat tgcgaagata
     3121 cttgaagcaa aatgccagaa aacaattggc gaacagataa ccggattgga tccgatgaac
     3181 acatataaga ttgggatccg tctttattgc aagaatttca taagaatcag aaaagaagaa
     3241 cgggatctgt tcatattcgg agaatcggtt gtcgaacaac tgccatgcag tgtgattgta
     3301 tatcaaaagg aaactccata aactatcacc gtttcatatg tgaccgacta taaagctcga
     3361 aagagctttt tcttttgatt ttcggctgta aatatccatt ctttgatctt tgtgatatat
     3421 tattattgtg aaaaccaaaa agaaatgatt taaaaaaccg ttttagtata tatatttacg
     3481 gtccgatact tatataaaaa tacacaatca gaaaaatact ttttgcagat gacatgcctg
     3541 gaaattcacg aaaaccgaat gggggatgaa aatgaaaaga tattgttggc tgattatgtt
     3601 tttattcgta ttattcatga cttcgtgtaa agccgcagag attcaatctt ccgtgacata
     3661 ttccgaattg atgtatgaaa caggagaaac aataacgctt tccgatactg atttgacggt
     3721 tatggtcgac aacgaaacga ttacggattt caccgtcaat attttgggaa caaacatcta
     3781 ttacgatgca ggtgattacc aagtagaaat cgagatcata ttacctgacg aaacttccat
     3841 cacgaccgta gtcacagtca cgattgtgga ttatcctctt tccgatatcc tgatgatttt
     3901 gggaataggc gagcacatcc atatcatcgg tgaggatgac gtcgatctga tggacggagt
     3961 gtcggccatt gatagaacat atggggatct gaccgcgaac atcatcgtcg atgattccga
     4021 tgtcgattat gagacgatcg gtgtctatgt tatttactat accgtgcaaa acgccgatgg
     4081 ttattcaaga agagaaacat ctaccgtcac agtcagactt ttcgatgtgc cggagacatt
     4141 tcccgaggaa tttgcaatag tgtattatgg cggtttatac ggagttgtag acatagacga
     4201 caatgagttg attccttgcg attataacga tataagttat cttgaagaag ggatcataga
     4261 gctggaaaaa gcgggatcgc tgttttattt caatctgtat accggagctt tcatcgaatt
     4321 cgacggaatg ccaaacggat ttactgaggg aagaagcgtg tttgtccttg atggcagata
     4381 cggaattttg agtatcgata acgaaatcat tgcgcccaat atctacagct ttgccaaact
     4441 gagggacata gaaggctatg cccaggtccg ctctggtgat tattacggat atgtcgattc
     4501 gtcgggagag gaagtcatcg aaccccgatt catttccatg accgatttgc ttccaaagaa
     4561 tgatttcgaa aacactttgt ttttggtccg cttcattgac ttgtcttacg gcatcatcga
     4621 ttccaacaac gatgtcgcgt attatctgac aaacagtacg attttaaaat atgaaatcga
     4681 tgaaaacaca tattacggaa tttatacggc tgacggcttg ataaccgtcg atgattcctt
     4741 cgccaaggaa acgttctcca tcgagaccga ttctttgcag attgtcgaaa tcgatgattc
     4801 cggtctcatg atcgtatgcg atagcaacgg ctcgtattat ctgttagaca aaaataagaa
     4861 tactttgttg tcggacattc ccttcgcttc gtttgccgat ttgtcatatg acgaatcgaa
     4921 ggaagtctat gtttttctca ataatgagaa cgtctatgtt tacgacacat cattcgtttt
     4981 ggtaaaagaa tatgacaaca gtttggatct gaatccggcc gacatcacca atatcgacac
     5041 ggagaatctg agataccaaa taaccgggaa tcacacattc caggttcttt acgatagttc
     5101 tggcaacacg gtttccttgg tgcgatatga cgatatctac atttatgacg attttgtggt
     5161 tttgtatgac gaattggctt tcgatatcgt attcgtcgac cggcagtttt catatatcta
     5221 cgacgagatc gagggaaccg gtatttttga accggattat tttgtggtaa acaatgccgc
     5281 ttcgactacg gccagcatct attcggatac cggcgctttg atcaagtcgg gtttgaatct
     5341 ggtgattgaa aacatcctca aggcccaaca cggcatctat atcatcaatc aaggcgaaac
     5401 caatgtttat ctgtatgacc aggtcgagga caatcttgtc cttatcgata ctgttcaggg
     5461 atacttaaat ttcataaacg gtatctacta ttcgttttat tgcgagtcgc aaacggtact
     5521 ttattcatat gccggcgggg tgctcacaaa aataaatgct tatgacatcg cgcaaggcgg
     5581 catgccagaa gccatcgatc tcggatacgg ctattatgtt gttccgagtt cggacgattt
     5641 gtattatgtc accaccccgg cgatcgatac ggaatatgat atctggatct tcccgcttag
     5701 cgaagatttc tatctggaag gaagttcaaa ccagatatcc ctcaaaaacc ggaacacgat
     5761 aaccttggac acctacaatt tcaccaattc ctatgatgac tatcccgggt ctttcacaaa
     5821 catcgttaaa attgaaatat cccccaccga atatcttgtc agattacaga gtttactggg
     5881 atattactat tggttctttt taagcgatac atcgattgct tccgttgatt tgacggaatt
     5941 ccaagaagat gacagaatcg cgtttgtcaa tacggataac gattactggt acatatactc
     6001 ctacaatcaa tcgacattga tatatgagtt gagcgaccag gcgacttttt tcagttatat
     6061 cgaatacgaa gacttgtacc tcattacaga cggaaacacc cacattgtca gaatcaatgg
     6121 cgacgatgaa tttgaataca tatatacgac atcgaattca attgtcgatt tgcacacaca
     6181 cgcggattat ctggcggttc aggaagaaac agacaaggtg atactgtatg aaaacagtct
     6241 tggacagaca gaaaagatca tgactatcgt tgatacgttg gcaacatttg agatcaatgg
     6301 agaaatcctt ttgggcgatc acgtcatttc gtttgacggg aacacatatc tcatcgacgg
     6361 agtgacaaaa gattacccgt cgatatattt cgattcgtcg accgatatac tatatggtat
     6421 tgacgaagac ggtatagatg cctattttgc aggttcgtgc gtaatgacgg ctaataccaa
     6481 cgtgatgtca ttgacttcgg gattttatta ttgttacgaa tcggggaaca tctcgttata
     6541 tgacggaaca gatttgttat tcacaataga cgccgatgaa atcgaatata tcgaagatgc
     6601 ggattcattc atggtgaaaa acgacggcac ttacggaatt atcgacatga cgggtgatta
     6661 tgtgatctca aatgattata tatccattgc gtttaatgat acctattaca tagtgactgg
     6721 atcttgttat tacggcgtgt atgacgaaaa tggaaacgaa ctgattgaca cccattacga
     6781 cgatttgtgg atctatattg ttttggaaga acaagactga tagaagtata taaatataag
     6841 ggcgtgactt atgatgaata tcgaagaagc gatttttgca agaagaagca ttcggacatt
     6901 taaacaagac gaagtgagcg atgaagcgat actgaaactg cttaaggcgg cgatggccgc
     6961 gccttccgcc tgtaacagga agccgtggct tttctatgtg gtaaaaaaca aagagaaaca
     7021 aatgcaactg cgacagatgc acagatacag cgattacgac tcaccgctca taatcatcgt
     7081 atgcgccgac atgagaagga aactgggggt aaaaagcgaa gacttttgga tccaggactg
     7141 ttcggcatcc gtcgagaatc ttatgctgga agcggtggga ctgggcatgg gaacatgctg
     7201 gtgcggcttg cacccgatcg aagccgccaa gaaacgggtc gtgaaaattc tgaatatgcc
     7261 cgagcatgtt gtccccgttg ccttgatcca tgtcggacac ccggccgagc atccgacgcc
     7321 aaggacccaa tacgatgaat cgctggtgac tgtcattgaa tagaatatgc agggcgaagg
     7381 ggaccgaaag atgaagaaaa tattgctggt tgactatgga agtacttatt tcaaagatgt
     7441 cattctcagc ttcgaacagt tgggtgtgga gtgtgtcaaa ttgcgttatg acgaaataac
     7501 cggtcatttg gataaaaaca atctatgcgg gatcgtcctg acgggaagtc ccggcagggt
     7561 gaacaatccg gaagatcccc aactggatcc ggctttgctg gaaacaggcg ttccgattct
     7621 cggaatctgt tacggcttgc aactgctcat gcattccctt ggcgggaaag tcgaagctct
     7681 ggataaaaga gatctggggt tcggtttgat gaccataatc aaggaatcgc cgataaacaa
     7741 aggactcggt aatccgacca atgtatggat ggcgcattac gaccatgtga caaagctggc
     7801 gaaaggattt gaaatctatg catcgacacc gatctcaatc gcgatggtcg gcgatgagaa
     7861 aaggaagatc tacggaatcc agtatcatcc ggaagccaaa agatcggatg acgatatgaa
     7921 acttctgaga aacttcacat atgatatttg cggatataca aacatgtgaa gaacaaactc
     7981 ttggataaaa caggagggaa aattatcgtg ttaagaatcg aacaactgac aaaatcatat
     8041 caatccggag aaaaaagaaa cattattctg gaaaaacttg atttttccgt tatccaaaat
     8101 gattttgtcg taattatggg aaaaagcgga agcggaaaga ccaccctgct ctattgcctg
     8161 ggtctgattg acgatatcga ttcgggtaca ataagtctaa acggcaagag aattgacaat
     8221 ctgggagaaa atgaaaaagc caaaatcagg ctgaatgaat tcggctttgt tttccaagat
     8281 atgaatttga ttaacatgct gacgcttaaa gagaatatcg ccatgccttc ttacgaacag
     8341 aaaacctatt cgagcgaaaa tgtcgataaa ttgattgatt cgttcggcct taccgacttt
     8401 gctgacaaaa aaccggatcg ggtcagcggc ggcgagaaac aacgggcagc gatcgccaga
     8461 gcgatcatta atcatccgaa aattttattt gcggacgaac cgaccggatc acttgattat
     8521 caaaactccc ttagtttgtt aaatcttttc aagaaaatga acaatgaagg ccagacattg
     8581 gtaattgttt cacacgacag attggttgcg agttatgcta aaaaaatata ttttcttcaa
     8641 gagaaaaaga ttcgacagat tcttgaattc gatcaagaga cggaagaaga acgtttttcc
     8701 gcgatcgaca aagctttact gggactggac tagcaagcaa tgaacagttt gttattaaga
     8761 aaaaatattt ggaagattct tatatctggc attttatttt tcttggtcgc tgccggtttt
     8821 caaattgctt tcaacctcac aaattctgat ttgaccctga cgtcgcggat gaagaaatac
     8881 aatgcatccc agacggaaat cgttctggac aaatattatg acttggatat gcatatatct
     8941 gatttttcta tggaaacgga gacttttgtt gctacttatc ccgggatatc ctattacacg
     9001 gcaagattga cttcaaaacg gcatgtcgtc cgttatgaag aatatgtcga caatgattat
     9061 aataactaca gagtgttgga aggaaagccg cttgatgaac ttgagttgta tgaagtggct
     9121 gtaccggcag ggtataatta ttatcttgat tcgaccaatg aggatggcct caacattggc
     9181 gatgaaataa ccgggtattc ccatccgctg attgtggcgt ccattgttga ttatatccaa
     9241 tatgtcgaac cggcattcaa tgaactgacc atgcaggaaa cagcaggaga tatgtcattc
     9301 gcttttgaat acatgcgttt tcttgcttcg ccagacacca tatcttacat aaacagtcag
     9361 ttaaccgata tacaatacca gtcgataatt tacaagataa ttttcgacga ttattcaaaa
     9421 gaagccgaaa aggaatttgt cagcgcttat ttggattatt ttgacggtgg caattcctat
     9481 attcacgtca atccgtttta cgaggtgctg gattcatcta acggtatgga tagggtgatc
     9541 ggaaaaaccg tgctgatcgc ggcattgact ttgctctgtt tcgggcttat cgctatcttt
     9601 gtagatatcc gtagggattt gaaccgggat cgtcgtcaaa tcggaattga aaaggcaacg
     9661 ggaatttcca ataacacgat tatccgttac tatttgtgga aattcggctt ggcgataggc
     9721 tttggtgttt tgcttgccat gatggttttt ttaatcattg ccattatctt gaaaaacaat
     9781 tatttctatt catttttctt atcaaaaaat ttcctttctt atttggaagg ttttgcaatc
     9841 attctcggaa tattcgggat cgcccttttg ttgatgaatt tggcattgaa acgcatcttg
     9901 actaaaacgc ctgctacttt cctaattcaa agcaattctt cagaacgagt aaaagccaac
     9961 cgtttgtcgg aaagaaatct acctgcaagg ctcggattca gattttttat ggccaatccc
    10021 gtttcatcga tggttcaggt gttggcaggc agcctggttg tcatagtaac ggcagttttt
    10081 ctactttttt ccggctttgc caagaacgtt tacacacctg ccaattacgg gcttgattat
    10141 gactatatga tcatagctaa atattcaaat tattcttata tcatggataa ttacggcgaa
    10201 tcgtctctta tcaccgcttc gaagattgtt tggatgcagg ttggcgacga aaacggggat
    10261 ccggtttatg tcaaagccat gtacgtcgct tactccggag atttatgtga ctttctcgca
    10321 tctgacatcg atgaagggac ttgtcctatc gactcgtcag agagaggact cggggtggtt
    10381 ggctatgcct tggccgaaga ctatcatctt aaggttgctg acgatgaagc agattcctta
    10441 agcaattaca tttggtttac cgaacagtct ctggccagta ccgatacgta taacattaaa
    10501 atctacggga tccaaacgat tttacaatat gatggttatg tcgtttttga ccgaatcggc
    10561 acctcggctg tttattcagg aagaaaattt ctagattttt atgaagccaa tactaccgcc
    10621 gcccaggttt atattaactt agaagccgga gccgacaagg atgctttaaa gacatatctt
    10681 gaaaataata atatctttta tgtttccggt gaggcaagtc aagcctcgat agatgactca
    10741 tcggccaagt tgatctttta tctgtccgga atcaccgttg ttggagccgt tattctgata
    10801 atgcttttca gccttgttgt aataatagat acttcggatt ttttctatcg aagacaaaag
    10861 gatttcggga ttttgagatc cttggggcaa agtcgaaaac gaatcatctc catgaatatg
    10921 tttcgccagg ggatagccgc tactttgttc tttgtcatcg gtgtggcaac agcgttttgt
    10981 cttagtaact taatcaggag aagttttgaa agaaccatgg gaatttatca tctagaaacc
    11041 ggcatgtgga caattgctcc tcttgttgtt gggatcctta tttttgttct tttccttctg
    11101 aatgctttcc tgttgtcgct tttctattcc aaagggccgg ttgcggattt ggttcgtcat
    11161 aacgactaag tttttttata taaattaagc aagcgaaaat gatatttttc ggttgctttt
    11221 ttatttgaaa aggaaaagat aatcatgctt tttgtatcag agataacgca ttcaaacatc
    11281 caaaatatct aattatataa caaaaaataa ttcgaccact ggttgcttta cctgagcttg
    11341 taatgcaatt tataatagaa ttcgaggtga tatggtgaaa tacaatatat tccagaacag
    11401 caagtatgta tatgaagctt cattccaaaa atgtcccagg ttgaagggcc tgttggctat
    11461 caactttttg tcagaactgt tgctgccatt cagtgcgaca ctagttacca ccgttgtcgt
    11521 ttatgcgctt accaaccaat tgcctgccgg cgaatatata tggattgttt tcggactcag
    11581 tctgtttaca ttcatattga ccggattgga atactggtcg tatacgacat acacattcga
    11641 aaacattttt acaaggaact cgactttcat gatcagactg accgatcatc agctcagcac
    11701 cgattatatg aacgtagagc caaaaagccg ccgcgatatc attagcaagg cgttcgaagc
    11761 catcggcagc aacatgtacg gcgtggaact gatgatgaga agcgcgccga cagccttgta
    11821 taatttcgtc ggcatgctga tttacggtgt gttgatcgtc atttactcgc cggtcattgt
    11881 ggcgatattg ttcgtgatga cggtcgtgaa tatatttttg acatcgagag ccaataaata
    11941 tctggcttca caaagagata gaatcgggaa aatatgggat gaaagatatt atctctcgca
    12001 agatgcgacc aatcataaat acgggaagga catcagggtt taccagatgc gcaattggtt
    12061 ttcccggata ttcgtcgctt tgaccaaaga aagaacattg atcgggaaaa aggtggaaaa
    12121 aagatttttg ttcgccagtc tctccaatac gatttttcta ttcattcgcg atcttgttgc
    12181 ttatgttact cttgccatcg cggtgttaaa cggatcgata gatgtgacta cattcacttt
    12241 ccttatcggt atcgtgaccg ggtttacggt gtggctgaac ggatttgttt cgcatttcaa
    12301 cgacctgaga aaagccaaca tcgcgacaaa cgaataccgg gaatgcttac agaccaaaaa
    12361 cgagtttaat cacggtgagg gcgtcgatac gggaaaaatg tcgaaaccga tcagcattga
    12421 gttcaaagat gtggaattca gttatcccga cagcgataat atgactttga aaaatatctc
    12481 gtttaaaatt gctgccggtg aaaaaatcgc catcgtcggc gacaacggag ccggaaaaac
    12541 caccattgtc aaattgcttt gcggtcttta caaacccacg aaaggcgaca tcttgattga
    12601 cggtgtgtcc atagacaaac tcaatcttga agaatacatg agactgcttt cggtggtttt
    12661 ccaggattcc gagcctttgg ctttcacgat cgaacagaat atcgcttgcg atgttccgga
    12721 aaacatcgat catgaccggg tacaaaaaag ccttgaggaa gccgatctga tcaagaaagt
    12781 cgaatcgcta cccctgaaag aaaaaacata tatcaccgag gaattcaaca aggacggtgt
    12841 taagctgagc ggaggcgaga tccagaaatt gatgcttgct agagcgcttt acaaacacgc
    12901 tcctattctg atactggacg aaccgacagc cgctttggat ccgatcagcg aagagaagat
    12961 gtatctgaga tacgaagact tcctgcaagg aaacaccagc gttttcatat cgcatcggtt
    13021 gtcatcgacc aagttctgcg accggatcct gtttataaaa gacggaaaag ttatcgaaga
    13081 gggcagccat tatgacctga tggccaaaga aggagagtac cgtgacttgt tccagataca
    13141 agcaaagtac tatcaggaag gaaaagatca tgacacagaa aaccagtctt aaaggcaata
    13201 tcaaaaaaat gtttcatgtc atgaatgttg tcaagcgtgt ttcgaaaggt tatttctatt
    13261 cgtttttagc agatagtttc tttagaagca tcaggccata tgtgaccatc atatatacgt
    13321 atttgatctt agacggcctg attgacaaaa tcccgcaaag ccaacttctc acctatgttt
    13381 tctggatggt cggattaaac gtcgtactgg aaataatcct gaagatcctc aattattcca
    13441 aagcgaaata tctgattgaa cttgacttca atctggaaaa ccagatagcc atcaaaacct
    13501 atgaattgga tttttgccaa atcgaagaca gcagcaatat gaaattgatc caacaagcca
    13561 aggaaggtag caacggtaac ggcggcctga gattgtattg cgaatatgtc atgaccggca
    13621 tgatcgcggc cgtcctaagc atcgcatacg gagtcatatt gttatccggg atgctgaaga
    13681 aagcgacgct tgtagatacc agcggcatag cacagttcct gaattcccct tattcgctag
    13741 ttatcatttt cgtatcaatg ttgattcccg gcatcatatc ctggttcatg atgaaactgg
    13801 ataataagaa atcatatgat gtcatgatgg gaaatatcga aggcaaccgg cgattctcat
    13861 actatttccg tatcgcatcc aattacaaat acggtaaaga tatccgcata tttggcatca
    13921 aggacatgat tttgaacgag atgaaagaca agaagaacag tgtcgacgca aactggcgcg
    13981 gttatgtcaa ctttgcggtc aggatcatga gcgtgacttt tttcgggaat atgttgttat
    14041 cgtttatcgc ttatgccatg gtcgggctca aagcaatcta cggtatcatc acgatcggaa
    14101 gcgtggtgtc atatgtggcg a
//
LOCUS       DAOPJL010000029        13485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_4795_length_13485_cov_8.027103, whole genome shotgun sequence.
ACCESSION   DAOPJL010000029 DAOPJL010000000
VERSION     DAOPJL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13485
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_13485_cov_8.027103"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLP02_04570"
     CDS             complement(<1..495)
                     /locus_tag="PLP02_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42747.1"
                     /translation="MKVSRFKFIIVSVIVLIMFMLTGCTETNADTDTTTSLFQGSTSG
                     LVFDNTGLESEFAVVNYLGTSENVIIGSTYYGYPVTSISADVFSNAKVTVTKISLPDS
                     IVMIEAGAFASTELEEFTVPVSLTYFAQAFGAHRGDISIKLAENHPTLKEVKIGGHKA
                     IVNLD"
     gene            complement(849..1064)
                     /locus_tag="PLP02_04575"
     CDS             complement(849..1064)
                     /locus_tag="PLP02_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HPF42748.1"
                     /translation="MPTWLEIVLPIFTLLVGGVVGFFLAKRSFKKYLEKNPPVNEKMI
                     RAMMTQMGRTPSEKQVRQVMASMNQAK"
     gene            1248..1415
                     /locus_tag="PLP02_04580"
     CDS             1248..1415
                     /locus_tag="PLP02_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPF42749.1"
                     /translation="MPRRITDACIACGSCKENCPVDCIEAGDIYVIDPEQCIDCGVCE
                     SNCPVEAIVAE"
     gene            1511..1954
                     /locus_tag="PLP02_04585"
     CDS             1511..1954
                     /locus_tag="PLP02_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42750.1"
                     /translation="MKTVILYYTFGGATMTEAERLGKELESSVYRIREIKKRCLFTSF
                     IPGGFQAMQQKKVPVTKPDVNLDEFDDIMIGCPIWANYPAPAFNSIVSWLPKGKHVDL
                     FFMSGSGNPLPHEKIIVQAILDKGCEVVSIRNVKTGKPPHKMKEK"
     gene            complement(2067..2510)
                     /locus_tag="PLP02_04590"
     CDS             complement(2067..2510)
                     /locus_tag="PLP02_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF42751.1"
                     /translation="MSKAKDLINELLVETFNRILTIEGEALKKEGVKLSMSEVHVLEA
                     VEKLPEPSMTAISQKLGITPGSLSVAINTLFQKGYVRRYTDNYDRRKVLVALEDKSKA
                     VLDIHQKFHQHMIDSVFSDLKLGEDEILIQLLKNVSDYFKRYQIE"
     gene            complement(2513..3367)
                     /locus_tag="PLP02_04595"
     CDS             complement(2513..3367)
                     /locus_tag="PLP02_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPF42752.1"
                     /translation="MANIGVAVCGNSGIDYLVHDKSIRIFHSLLMIGTQEYEDFVEIS
                     CDEFYARLEKNPDLDIRTAQTSTGKLLEMYKEMIASGYDELLIITISKHLSGTYQNAV
                     LAAKMIENVPIHIFDSQSLSYVEALMAMTARLMADEGEKVPDIMRKLEWIRDHNHIFV
                     TVDTLKYLIKNGRLSNAAGFFGTLLKLKPLLEVSKDGKVETLEKIRTTQRARAEMMDL
                     FYKEIAGKKVVAFIVYTNNMNEFFDLKDLLEERPEIVEMKMIPLTPVVGCHAGPGTMG
                     LGYIEKEE"
     gene            complement(3474..4238)
                     /locus_tag="PLP02_04600"
     CDS             complement(3474..4238)
                     /locus_tag="PLP02_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPF42753.1"
                     /translation="MNRIEVTKYLETMYPRHLAYDWDNVGLQVGTLNKPVKKILVTLD
                     VTKDVVREAADLKADLILSHHPLIFQPISSVQFETPKGWIIQELVKKDIALYSMHTNF
                     DVADGGMNDRLSERLGLRDTKLLDEELGIGRYGRINPMPMPEFISYVKKQLEVTHVRL
                     IGNMNKTVEIVGISGGSGSQHMHQAKKRGCDVYITGDVTYHNALDAIMMGYTIIDVGH
                     NVEKIFIPSIIDDIRKEFPYLDIAASTVDTNPYQEK"
     gene            complement(4235..4891)
                     /locus_tag="PLP02_04605"
     CDS             complement(4235..4891)
                     /locus_tag="PLP02_04605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF42754.1"
                     /translation="MTQLSPRLGAVLSFCHGFDCLIDIGSDHAYLPIEAVRQGYVAKA
                     IAVDNKPSPLSVALKNIEQSGLKDKIETVLSDGFTSLNHSGDLVTISGIGGKLTAEIL
                     EQGDLEKVKMLVLSPNSEAFVVRDWLEKNHFVITGETFLLDRGKHYQVIAAKKGVMEL
                     DLIEKEFGPMIIREKNPAFVAYIRKIIAKLDKAAAGANDIQEKERIRERIGLLKGLVL
                     "
     gene            complement(4888..6201)
                     /gene="rpoD"
                     /locus_tag="PLP02_04610"
     CDS             complement(4888..6201)
                     /gene="rpoD"
                     /locus_tag="PLP02_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPF42755.1"
                     /translation="MDKKEIVAKLVKQHEDEGSIGIDDVKKYAEEGSKLFEDIIVDLE
                     TEGIDLYDDNDLGEIDDGLIDDEDEEKQEEKPAEDEDAEEDDFVDLDELSDEDIPEEE
                     LIDFDYAKEIEEELMLEKQFDSNVSIKVDDPVRMYLKEIGRVELLSNDEEYELATLIS
                     RARKAKETYEERVKNGETISSEEMADYEELFKRGEFSEKKLVEANLRLVVSIAKRYVG
                     RGMQFLDLIQEGNMGLMKAVYKFDHTKGFKFSTYATWWIRQAITRAIADQARTIRIPV
                     HMVETINKLVRTQRTLTQKLKREPRPEEVAAEMHISVDKVQIIQKVAQEPISLESPVG
                     EEEDSSLGDFIPDPDTLTPYEYTSKEMLKRELDSVLETLTDREEKVLRMRFGLLDGRT
                     RTLEEVGKEFGVTRERIRQIEAKALRKLRHPSRSKKLKDFTNGNK"
     gene            complement(6183..7982)
                     /gene="dnaG"
                     /locus_tag="PLP02_04615"
     CDS             complement(6183..7982)
                     /gene="dnaG"
                     /locus_tag="PLP02_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPF42756.1"
                     /translation="MARISQDTVDRIKDAADILDVVGEYVALQPAGKNHKGLCPFHNE
                     KTPSFFVSPERNWFHCYGCGAKGDPITFIQKYKNISYVDSLKYLGEKYNIEMKWEGDI
                     ETSVSRDRLYQINEEAMRFYQLYLTNTEKGKVPLAYLSKRGIGIHTVQYFEIGYAPKE
                     SDALYQHLKEKYTELDMLELGLIKKKDTGEYYDLFRDRVVFPLKNAYGKVLGFSGRLY
                     EAKEDEPKYVNSPFTDIFIKGETLYNLDKAQTFIRQSGEAILYEGFMDVIASVKAGVK
                     NAVASMGTQLTMQQAALLRKYTDKVVICYDGDEAGFNATGKAITILRDERFEISVVVL
                     PEGLDPDDFVRKHSAKAFAEFIDKNKIDIYEFAYLHFLHGHDLSKASEAEAFKLKVFD
                     FLQKKASITVTDIYLKKMASTIGVDESSIKADYESYLMMQAIKKSLVTRKTHNQFSTV
                     THRYVKAEEILINYYLKSAVFRKEIRDNLTDLFAKEKGNVEIIGCAIDIADTSDKDDI
                     TAEVFAQIPESRHELFHRKLLDPGYEYSSQELSDCIMTIKDRKVEEMVAELEDEKNSI
                     TYENKKERYIELVNQIAELKRSQGKDKKWIKKK"
     gene            complement(8016..9398)
                     /locus_tag="PLP02_04620"
     CDS             complement(8016..9398)
                     /locus_tag="PLP02_04620"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPF42757.1"
                     /translation="MIDLANTLDQLVNYCKQYGFIFQGSEIYGGLANSWDYGPLGVEL
                     KNNIKKAWWEKFVARNPYNVGLDSGIILHTNAWIASGHVKSFQDPLMDCKECKSRHRA
                     DKLIEAHSKGKVDADGWSRDEMLRYINDNKVKCPVCGAFNYTDIRQFNLMFKTHQGVI
                     EDEQSQVYLRPETAQGIFLDFKNVLRVSRKKIPFGIGQIGKAFRNEITPGNFVFRTRE
                     FEQMELEFFCKPHTELEWFAYWKDFAQKFLLSLGLDPEHIRFHDHPKEKLSFYSNATT
                     DILYQFPWGFDELWGIASRTDYDLGRHQENSGENLEYLDPDTNEKYLPYVIEPSLGVE
                     RLLLAILNDALTEETVESTGETRTLLKIDKHLAPYQVAVLPLVKKYHQEKSNAVFNML
                     INDFSAVYDDTGNIGRRYRRQDAIGTPYAVTIDDTTESEGTVTIRDRDSMSQVVIAVA
                     ELSDYLKKQF"
     gene            complement(9489..10208)
                     /gene="recO"
                     /locus_tag="PLP02_04625"
     CDS             complement(9489..10208)
                     /gene="recO"
                     /locus_tag="PLP02_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPF42758.1"
                     /translation="MTETTGFVLKRMDYKETSKIIFVYTPTGRESYLVHGANKFTSPF
                     LAQAEILNLLNVKSEGKGLKTAKNIETKARYPHIKDNLEKYTYALHLFEILQNVADAE
                     IDHAKLFGFMEKLLPKMEESDNYIPYAYMFESKLLYLLGIQPEFRECVVCKSKEVAGF
                     SVPDGGMLCQEHYRESCYPLTLAREWMRLYYFDINKEAEPPLLSYDTAVGLRRLLDDY
                     YRYHLNMRTKSRQVLEGLLGY"
     gene            complement(10219..11112)
                     /gene="era"
                     /locus_tag="PLP02_04630"
     CDS             complement(10219..11112)
                     /gene="era"
                     /locus_tag="PLP02_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018393962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPF42759.1"
                     /translation="MFKSGFIAIIGEPNVGKSTFLNQVLKKKIAITSDKPQTTRDVFA
                     GIFNDSESQLVFLDTPGIHNAKTKLGEYMTSSAISTIKAVDLVLFFVNAYDDVKESNL
                     KIMDKLENIKTPVFLIINKLDTITDFQRLEAAIKPYKDKCAFAGVFAVSALTGQYVDL
                     LMKDIKNMLPEGPKYYPDGALSDRPETFLIQEFIREKILDNTREEVPHAVAVYIERMQ
                     HKKELTRIDATIVVERDSQKAIVIGKNGARLKKIGTDARLDIEELLGRSVYLDLFCKV
                     EPNWRNRDHYLKTYGYKPEKK"
     gene            complement(11112..11504)
                     /gene="cdd"
                     /locus_tag="PLP02_04635"
     CDS             complement(11112..11504)
                     /gene="cdd"
                     /locus_tag="PLP02_04635"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPF42760.1"
                     /translation="MDKLIKMAKENIVNSYSPYSKFAVSAVLELKNGDTYKGVNIENA
                     AFGLSNCAERSALFAAYSNGIKKEDIKKIVIFTDHPYFVSPCGSCRQVMRELMEDDAD
                     IILVNNKNETKTIKNSELLPFGFSKKDL"
     gene            complement(11511..11939)
                     /gene="ybeY"
                     /locus_tag="PLP02_04640"
     CDS             complement(11511..11939)
                     /gene="ybeY"
                     /locus_tag="PLP02_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPF42761.1"
                     /translation="MLRINYVDEASSPEECHEIIRSILKKAARETRLCGYKIISVIMV
                     TNEKIHELNRIYRNVDRPTDVLSFPDDETKRELGDIFISLEKTKSQAEEYGHSFDREL
                     AFLALHGFLHCLGYDHLDKESETEMFTLQKQILEKSKYKR"
     gene            complement(11926..12894)
                     /locus_tag="PLP02_04645"
     CDS             complement(11926..12894)
                     /locus_tag="PLP02_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPF42762.1"
                     /translation="MVDLTKLDIPVTNVSEMVALFGVNEKYVRILSGLYGFNIYVNHE
                     GILLDTDDEEKISEIRGIFDILLQLVKRNIVFNERDIIYIHHMYNIAGKEELLSLFLG
                     RQEVVKTFSGKPVYAKTLSQKTYLDAIEKNTVVFGIGPAGTGKTYLAVLYGLRKLKEG
                     KIKKIVLTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDAMYEVLGLRLTEEYIEKGLI
                     EIAPLAYMRGRTLENVYVILDEAQNTTVGQMKLFLTRLGFQSKMIVTGDVTQSDLPAR
                     VPSGLIRASEILKEINDIAIVTFDNTDVVRHPLVQTIIKRFEDAKD"
     gene            complement(12976..13152)
                     /gene="rpsU"
                     /locus_tag="PLP02_04650"
     CDS             complement(12976..13152)
                     /gene="rpsU"
                     /locus_tag="PLP02_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPF42763.1"
                     /translation="MAKTVVRDNESIEDALRRFKRDVSRAGTLAEARKREYYLKPSVS
                     KKLKQRQHKGGHRV"
     gene            13295..>13485
                     /locus_tag="PLP02_04655"
     CDS             13295..>13485
                     /locus_tag="PLP02_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42764.1"
                     /translation="MDLPMFYFMNLGLVQIIIIAVAAIAFIILLAVFINKGRYAARYK
                     NFYKKLDRTINKNKRYNGN"
BASE COUNT         3254 a         3038 c         3074 g         4119 t
ORIGIN      
        1 gtccagattt acgatggctt tgtggccgcc gatctttact tccttgagtg tgggatggtt
       61 ttccgctagt ttaatcgaaa tatcacccct gtgagcaccg aatgcctgag cgaaatatgt
      121 caaactgacc gggaccgtga attcttccaa ttcggttgat gcaaaagccc ccgcttcaat
      181 cataacgata gaatctggca aagatatctt ggtcactgtg actttggcat tggaaaaaac
      241 atcggcagag attgatgtaa caggataacc gtaataagtc gatccgatga tcacgttttc
      301 agaagtgccc aaataattga ccacggcaaa ctcgctttca aggcccgtat tatcgaaaac
      361 caatccggaa gttgaacctt ggaaaagcga agtagtcgta tctgtatcag cgttcgtttc
      421 ggtacatcct gtaagcatga acatgataag gacgataacg gatacgatta taaatttgaa
      481 tctagacact ttcatatttt catccccttt cattagttaa gcaaatcaca taataatatg
      541 tccttcattt tcgatgcttt tattctggta ttagctatta gcatcagtaa caaataaaac
      601 cggcgataaa aaaaacgcct caaataatcg tgtcatatta ataaaataca gtactgattg
      661 ttataactta cattcatttt atttcattag ttacttattg taaacagggt ttttattttt
      721 tgtgctttca tttgcatatt attttataat tctgttctcg tttaatttga acaaaaaaat
      781 cccgtatcgc ttgccacaca ttttttgtgt gtccgcagta cgggatattc ttaattgtat
      841 tctcggtatt atttagcttg gttcatcgaa gccataacct gacgaacctg tttttccgaa
      901 ggagttcttc ccatctgtgt catcatcgca cggatcatct tttcgttaac cggcggattt
      961 ttttccaaat actttttaaa cgatcttttc gccagaaaga atccgacgac acctccgaca
     1021 agcaaagtaa atatcggcaa tacgatttca agccatgttg gcattttcat cactccttct
     1081 tttcttactt tattttatca aaaaatcctt gttaaaacaa gataaattgc tttgaaacaa
     1141 tcatgaaata taaccggcaa aagaaaaatg cacctttaca aatagtcgcc gtgttggtaa
     1201 aataataggc gtagtttacc aatcatcagt caagggaggg aatgaagatg ccaagaagga
     1261 ttacggacgc atgcatcgcc tgcggatcat gcaaggaaaa ttgcccggtc gactgcatcg
     1321 aagccggaga tatctatgtt atcgatcccg aacaatgcat cgattgcgga gtttgtgaat
     1381 caaactgccc ggttgaagcc atcgtcgccg aataaacgag gcctacacca taagaatctc
     1441 taaacatgaa agagcctcac ttgtgtcaaa gacgacagag gcttttttgt tttcatgaaa
     1501 ggatggacat atgaaaactg tcattctgta ttacaccttc ggcggcgcca ccatgacgga
     1561 agcggaaagg ctcggaaaag aactagaaag ttctgtctac cgcatcaggg aaatcaagaa
     1621 gcgctgtctt ttcacttctt tcattcccgg tggctttcag gccatgcagc aaaaaaaggt
     1681 accagttaca aagcctgacg tcaacctgga cgaattcgac gacatcatga tcggctgtcc
     1741 gatctgggcg aattatccgg ctccggcttt caattcgatt gtcagctggc taccaaaagg
     1801 aaaacatgtg gatctttttt tcatgtccgg aagcggcaat ccgcttccgc acgagaaaat
     1861 aatagttcag gccattcttg acaaaggctg cgaagttgtt tcaatcagaa atgtcaaaac
     1921 aggcaaaccg cctcacaaga tgaaagaaaa gtaatcaata ataaaaaaga aaaatcccct
     1981 gttatatcat cctatgatga cacaagaaca gggatttttc tttatcctat tgaaaagaga
     2041 ggagaaacaa tgctttttga cagatactat tcgatttggt aacgtttgaa gtaatcggaa
     2101 acatttttca gcaactggat cagaatctcg tcttctccga gtttcaaatc agaaaacacg
     2161 gaatcgatca tgtgctgatg gaatttctgg tgaatgtcca gaactgcttt gcttttgtcc
     2221 tccaatgcga caaggacctt tcttctgtcg taattgtcgg tgtagcgacg gacataaccc
     2281 ttctgaaaca aagtgttgat cgcaaccgaa aggcttcccg gagtgatacc gagtttttgg
     2341 gagatggcag tcatcgacgg ttccggcaat ttctccacag cctcgaggac atggacttcg
     2401 gacatcgaaa gtttgacgcc ttcttttttc agggcttcac cttctattgt caaaatccgg
     2461 ttgaaagtct cgaccaaaag ttcgttgatc aaatcttttg ctttcgacat ggctattcct
     2521 ccttttcgat gtagccaagt cccatcgtcc ctggtccggc atgacagccg accacggggg
     2581 tcaggggaat cattttcatt tcgacaatct caggacgttc ttccagaaga tccttgaggt
     2641 cgaaaaattc gttcatgttg ttagtgtaaa caatgaatgc gacaactttt ttgccggcaa
     2701 tctctttata gaaaagatcc atcatttccg ctctcgcacg ttgcgttgtc cttatctttt
     2761 ccaaggtttc gaccttgccg tccttggata cttccaacaa cggttttaat ttcagcaatg
     2821 tcccgaagaa tccggctgca ttcgaaagtc ttccgttttt tatcagatat ttaagcgtgt
     2881 ccaccgtgac aaaaatatgg ttgtggtcgc ggatccattc gagctttcgc atgatgtcag
     2941 gaactttttc accttcatcc gccatcagtc tggctgtcat tgccatcagg gcttcgacat
     3001 aggaaagcga ttgtgaatca aaaatatgga tggggacatt ctcgatcatc ttcgctgcca
     3061 gaacggcgtt ctgataagtg ccggaaaggt gtttggagat ggttatgatt agaagttcgt
     3121 catatccgga agcgatcatt tctttgtaca tttccagaag tttgccggta cttgtctggg
     3181 ctgtcctgat gtcgagatcg ggattttttt ctagtctggc gtaaaattcg tcacaggata
     3241 tttccacgaa gtcctcgtat tcctgggttc cgatcatcag caaggaatga aaaatgcgaa
     3301 tggatttgtc gtgtacgaga taatcgatgc ctgaattgcc gcatacggct acgcctatgt
     3361 ttgccatgat aatggtctcc tctgtttgtt ttaatatcaa atgttttgaa tttcaaacag
     3421 agtataccat gatatctttt ttattgcaag taaaaaggct gattttcaca gccttatttt
     3481 tcctgatagg gattggtgtc aacagtggag gccgcaatat ccaagtatgg gaattctttc
     3541 ctgatgtcgt cgatgattga cggaataaat attttttcaa cgttgtgtcc gacatcaatg
     3601 atcgtataac ccatcatgat ggcgtcgagg gcattgtggt atgtaacgtc cccggtgatg
     3661 tagacgtcgc atccgcgttt tttcgcttga tgcatgtgct ggcttccgga accgccggat
     3721 atgccgacaa tctccaccgt tttgttcatg ttaccgatta ggcgcacatg cgtaacttca
     3781 agttgtttct tgacatatga aatgaattcc ggcatcggca tgggattgat cctgccgtaa
     3841 cgtccgatgc ctaattcttc atccaaaagt ttcgtgtctc ttagcccgag tctttcggaa
     3901 aggcggtcat tcatccctcc gtctgcaaca tcgaaattcg tgtgcataga atagagggcg
     3961 atatcctttt tcacgagttc ctggattatc cagcctttcg gagtctcgaa ctgaaccgaa
     4021 ctgatcggtt ggaatatcaa agggtgatgc gataagatga gatccgcttt gaggtcagcc
     4081 gcttccctga caacatcctt cgtgacatca agcgtcacaa gaatcttctt cacgggcttg
     4141 ttcaaggtac caacctgcaa cccgacattg tcccagtcat atgcaagatg acggggatac
     4201 atggtttcta agtatttggt aacttcgatt ctgttcacaa taccagtcct ttcaacaggc
     4261 cgatcctttc ccggatcctt tctttttctt ggatatcgtt ggctcccgct gcggctttgt
     4321 cgagtttcgc aatgattttt cttatgtatg cgacgaaagc gggatttttc tccctgatta
     4381 tcatcgggcc gaattctttc tcaatcagat ccagttccat gacgcctttt ttggcggcaa
     4441 tcacctgata atgcttgccg cgatccaaaa gaaatgtttc gcctgtaatc acgaaatgat
     4501 ttttctccag ccagtcacgg acgacaaatg cttcggaatt gggagacaaa accagcattt
     4561 tgactttttc gagatcgcct tgttccagga tctcggccgt tagttttccg ccgattccgg
     4621 aaatggtgac gagatcgcca gaatggttta gagatgtgaa gccatccgac agaacggtct
     4681 cgattttgtc ttttaggccg ctttgttcga tgttctttaa agcaacgctt aagggcgatg
     4741 gcttgttgtc gacggcgatg gctttggcca catatccctg ccttaccgct tcgatcggca
     4801 gataagcatg gtcggagccg atatcgatca ggcaatcgaa gccatggcag aaggaaagaa
     4861 cggcgccaag ccttggggac aattgtgtca tttgtttccg ttcgtaaagt ctttcagttt
     4921 cttgctgcgg gaaggatggc ggagtttgcg aagcgctttc gcttcgatct gccggattct
     4981 ttctctggtc actccgaatt ccttgccgac ttcttccagg gtcctggttc tgccgtcaag
     5041 caggccaaac cgcattctca ggactttctc ctcgcggtcg gtcagggttt ccaatacgga
     5101 atcgagttcc cgtttcaaca tttccttgga agtatattcg taaggagtca gggtgtcggg
     5161 atcaggaatg aaatctccca aagaagagtc ttcttcctcg ccgaccggac tttcgagtga
     5221 aatcggttcc tgagcgactt tctggatgat ctggaccttg tcgaccgaaa tatgcatttc
     5281 cgccgcgact tcttcgggtc ggggttctct tttcagtttc tgagtgagcg tcctttgagt
     5341 tcttacgagc ttgttgatgg tttcgaccat gtggacgggg atcctgatcg ttcgtgcctg
     5401 atcggcgatc gcccgggtga tggcttgacg aatccaccat gtggcgtagg ttgaaaactt
     5461 aaaacccttg gtgtggtcga atttgtaaac ggctttcatc agacccatgt tgccttcctg
     5521 gataagatct aggaattgca tgcctcttcc gacatagcgt ttggcgatcg agacaaccag
     5581 acggagattg gcttcgacca gttttttttc gctgaattcg ccgcgtttga agagttcttc
     5641 ataatcggcc atttcttcgc tcgagatagt ctcgccgttt ttcacgcgtt cctcgtatgt
     5701 ttccttggct ttgcgagccc ggctgatcag ggttgcaagt tcgtattctt cgtcgttcga
     5761 gagaagttcg acccggccga tctctttaag atacattctg acaggatcat caaccttgat
     5821 cgatacatta ctgtcgaatt gcttttcaag catcaattcc tcttcgattt ctttcgcata
     5881 atcaaaatcg atcaattcct cttcgggaat atcttcatcg gagagttcgt ccaaatcgac
     5941 aaaatcgtct tcttccgcat cttcgtcttc ggctggtttc tcttcctgtt tttcctcatc
     6001 ttcgtcgtca atcagtccgt cgtcgatttc gccaagatcg ttatcgtcat ataggtcgat
     6061 tccttcggtt tcgagatcga ctatgatatc ttcaaacagt ttcgatccct cttcggcgta
     6121 tttcttgaca tcatcgatgc cgatggatcc ttcatcctcg tgctgtttga ctaattttgc
     6181 gactatttct tttttatcca ttttttatcc tttccctgcg accttttgag ttcggcgatt
     6241 tgatttacaa gttcaatata gcgttctttc ttgttttcgt atgtgatcga atttttctcg
     6301 tcctcgagtt cggccaccat ttcttcgact ttgcgatctt tgattgtcat gatgcaatcg
     6361 gacagttctt gtgacgagta ttcgtacccg gggtccaaaa gtttacgatg aaacaactcg
     6421 tggcggcttt ccggaatctg ggcgaacact tccgcggtaa tgtcgtcttt atccgaggtg
     6481 tcggcaatat caatagcgca cccgatgatc tcgacgttgc ctttttcttt agcaaagaga
     6541 tcggttagat tgtcgcggat ttccttgcgg aagacggcgg atttcaaata gtagttgatc
     6601 aggatttcct cggctttcac atagcgatgc gtcacggtcg agaattggtt gtgggttttc
     6661 ctggtgacta ggctcttttt gattgcctgc atcatcagat atgattcgta gtcggcttta
     6721 attgatgatt cgtcgacgcc gatcgtggaa gccatttttt tcagatagat gtccgtgacc
     6781 gtgattgacg ctttcttctg taagaaatca aaaactttaa gtttaaacgc ttccgcttcg
     6841 gaagccttgg aaagatcatg tccgtggaga aagtggagat aggcgaattc gtagatatct
     6901 atcttattct tatcgataaa ctcggcaaaa gccttggcgg aatgcttacg gacaaaatcg
     6961 tccggatcga ggccttccgg taatacgaca acgctgatct cgaatctttc atccctgagg
     7021 atggtaatgg ctttgccggt ggcgttaaag cccgcctcgt cgccgtcgta acaaatgaca
     7081 accttatccg tgtattttcg caaaagggcc gcctgttgca tggtcaactg ggttcccatc
     7141 gaagcgacgg cattcttgac gccggctttg acggaagcga tgacatccat gaaaccttcg
     7201 taaagaatcg cttcgccaga ttgtcttatg aatgtctgcg ccttgtcgag attgtacaag
     7261 gtttcccctt ttatgaaaat gtcggtaaag ggagagttga catatttcgg ttcgtcttcc
     7321 tttgcctcat aaagccgtcc gctgaatccc aggacttttc cgtaggcgtt tttcagggga
     7381 aacacgacgc ggtcgcggaa aaggtcgtaa tattcaccgg tgtctttttt ctttatgaga
     7441 ccgagttcga gcatgtccaa ttccgtatat ttttccttga gatgctggta aagcgcatct
     7501 gattccttgg gggcgtatcc gatctcgaaa tactggacgg tatgaatgcc gatgccgcgt
     7561 ttggaaagat aagcgagcgg aactttgcct ttttccgtgt tggtgagata caactgataa
     7621 aagcgcatgg cttcctcgtt gatctggtac aaacggtcac ggctgaccga cgtttcgatg
     7681 tcgccttccc atttcatttc gatattgtat ttctcaccca gatatttcag gctgtcgaca
     7741 taggaaatgt tcttgtattt ctggatgaaa gtgataggat cgcctttggc gccacatccg
     7801 taacaatgga accagttacg ctcgggagac acaaaaaaag aaggtgtctt ttcgttatgg
     7861 aaagggcaaa ggcctttatg gttcttgccg gccggttgca aggctacgta ttcgccgaca
     7921 acgtcgagaa tgtcagccgc atccttgatc ctgtcaacgg tgtcctgact gatccttgcc
     7981 atttcctcac cttctttttg gttatttttt atgaatcaga attgtttttt caaataatcc
     8041 gacaattcag cgacggcaat tacaacctga ctcatggaat cgcggtcacg aatggtgacg
     8101 gttccttcgc tttcggtcgt atcgtcgatt gtcacggcat acggagtgcc gatggcatcc
     8161 tggcggcgat aacggcggcc gatattgccg gtgtcgtcat agacggcaga aaaatcattt
     8221 atcagcatgt tgaagactgc gtttgatttt tcctgatgat atttcttgac gagcggcagg
     8281 acggcaacct ggtatggagc cagatgcttg tcgattttga gcagcgtcct ggtctcgccg
     8341 gtcgattcta cggtttcttc cgtcagagcg tcgttcagga ttgcgagtaa cagcctttcg
     8401 actccgagcg acggttcgat gacataggga agatatttct cgttagtgtc gggatcaaga
     8461 tattccaggt tttctccgga attttcctga tgtcttccga gatcgtaatc ggtacgggaa
     8521 gcgatgcccc agagttcgtc gaacccccag gggaattggt acaatatgtc ggtggtggcg
     8581 ttggaataga acgaaagctt ttctttcgga tgatcatgga atctgatatg ttccggatca
     8641 agccccaaag ataacaggaa tttttgggcg aaatccttcc agtaagcgaa ccattcgagt
     8701 tccgtatgcg gtttgcaaaa gaattccagt tccatctgtt cgaattccct tgtacggaaa
     8761 acaaagttcc ccggagtaat ttcgttacgg aaagccttgc cgatctgacc gatgccaaat
     8821 gggattttct tccgcgatac ccttaagaca tttttgaaat caaggaagat tccctgagcg
     8881 gtttccggac gcagatatac ttgactttgc tcgtcctcga tcacgccctg atgggttttg
     8941 aacatcagat tgaactgtct gatgtcggta taattgaaag cgccgcaaac agggcacttc
     9001 actttgttat cgttgatata tcggagcatt tcgtcacggc tccaaccgtc ggcgtccact
     9061 ttgcccttcg aatgggcttc gatcagctta tcggcccgat gtctcgattt gcattccttg
     9121 caatccatga gcggatcttg gaaactcttg acgtgtccgg atgctatcca ggcgtttgta
     9181 tgcaaaatga tcccggaatc gagaccgacg ttgtatggat tcctggcgac gaatttttcc
     9241 caccaggctt ttttgatgtt gtttttgagt tcgacgccta gagggccgta gtcccaggaa
     9301 ttggcgaggc cgccgtagat ttcggagccc tggaagatga atccgtattg tttgcagtag
     9361 ttcacaagtt gatcgagtgt gttggccaaa tcaatcatcc tcccaaaatg gcgcatatta
     9421 cttcagtata attatattat tattaactgt attgtcaagt tgatatatgc aaattgaaaa
     9481 caagggactc agtatcccag cagtccttct aggacctggc gtgactttgt cctcatgttc
     9541 aggtgatatc ggtaatagtc atcgagaagc cttcttaacc cgactgctgt gtcataagac
     9601 aacagaggcg gttcggcttc tttgttgatg tcgaaataat aaagacgcat ccattctctt
     9661 gcaagtgtga gagggtaaca ggattcgcgg taatgttcct gacaaagcat tccgccgtcc
     9721 ggcacggaaa atccggccac ttccttgctt ttgcagacga cacattccct gaattccggt
     9781 tgaatcccca aaagatataa caacttgctt tcaaacatat aggcgtaagg aatgtagttg
     9841 tcgctttctt ccattttagg aagcaatttt tccatgaatc caaacaactt tgcatgatcg
     9901 atttcggcat cggccacatt ctgaaggatt tcaaaaagat gcagggcgta ggtatatttt
     9961 tccaggttgt ctttgatgtg gggataacgt gcctttgttt cgatgttttt ggctgtcttc
    10021 agtccttttc cttcgctttt cacgtttaaa aggtttaata tttccgcttg ggccaggaat
    10081 gggcttgtga acttgttggc tccgtggacg agatagcttt cacggcccgt cggggtatag
    10141 acgaaaatga tcttgctcgt ctctttgtaa tccatccttt tgagaacgaa tcccgtcgtt
    10201 tcggtcatgt ctaacacctt atttcttttc tggtttgtat ccgtatgttt tcagataatg
    10261 gtctcggttc cgccagtttg gttcgacttt gcagaaaagg tcgagataga cggaacgtcc
    10321 cagtaattct tcgatgtcca atctcgcatc ggtcccgatc tttttgagcc tggcaccgtt
    10381 tttgccgatc acgatcgctt tttgggaatc gcgctcgaca acgattgtcg catcgattct
    10441 ggtcagttct ttcttgtgtt gcatcctttc gatatagacc gctacagcat gaggaacttc
    10501 ttcacgggta ttgtccaata ttttttcacg gatgaattcc tggatcagga atgtctccgg
    10561 ccggtcggaa agagcgccgt ccggataata tttcgggcct tccggcaaca tgtttttgat
    10621 gtctttcata agcaaatcga catattgccc ggtcaaagcg gaaaccgcaa agaccccggc
    10681 aaaagcacat ttgtccttat agggtttgat agcggcctca aggcgctgaa aatcggtgat
    10741 cgtgtcaagc ttgttgataa tgagaaaaac cggcgttttg atgttttcca gtttgtccat
    10801 tatcttgaga ttgctttctt ttacatcatc gtaggcgttg acaaaaaaaa gcaccagatc
    10861 gacagccttg atcgtgctga tggccgatga tgtcatatat tcgcccagtt tggtcttggc
    10921 attgtgaatt ccgggcgtat cgagaaaaac cagttgcgat tcactatcgt taaagatacc
    10981 ggcgaatacg tctctggtcg tttgcggctt gtccgaggtg attgcgatct tctttttcag
    11041 gacttggttc aaaaacgtcg atttgccgac gtttggttcg ccgattatgg ctataaatcc
    11101 ggatttgaac atcagagatc cttttttgag aaaccgaatg gcaacaattc ggagtttttg
    11161 atcgttttgg tttcgttctt gttgtttacc aaaatgatat cggcatcgtc ttccatcaat
    11221 tcacgcatga cctggcgaca ggatccgcaa ggggacacga aataaggatg gtcggtgaaa
    11281 atgacgattt ttttgatatc ttctttcttg attccgttgg agtaagctgc gaaaagagcg
    11341 cttctttcgg cgcagttcga cagaccgaaa gcggcgtttt cgatgttgac gcccttgtaa
    11401 gtatcgccgt ttttaagctc gagaaccgcc gacacggcga atttggaata gggtgaatag
    11461 gaattgacga tgttttcttt cgccattttg attagtttgt ccattttgca ttacctcttg
    11521 tatttcgatt tttccagtat ctgtttctgg agggtgaaca tttccgtctc ggattccttg
    11581 tccaagtggt cgtatcctag gcagtgaaga aatccatgta aagcgagaaa agcaagttcg
    11641 cggtcgaatg aatgtccgta ttcttcggcc tgcgatttgg ttttttcgag cgatatgaat
    11701 atgtctccga gttctctttt tgtttcgtcg tcgggaaaac tcagaacatc ggtcggccgg
    11761 tcaacgttac ggtatatccg gtttagttcg tggattttct cgtttgtgac cattatcacg
    11821 ctgatgattt tatatccgca tagcctggtt tccctggctg cttttttcag aatgctacgg
    11881 attatttcat gacattcttc cgggctggaa gcttcatcca cataattaat ccttagcatc
    11941 ttcgaacctc ttaatgatcg tttggacaag cggatgtctc acgacatcgg tgttgtcgaa
    12001 tgtaacgatc gctatgtcgt tgatctcttt taggatctcg gaagccctga tcaggcccga
    12061 cggaactctt gcgggcaaat cgctttgagt gacatcgccg gtgacgatca ttttggattg
    12121 gaatcccagc ctcgtcagga acagtttcat ctgcccgaca gtagtgttct gggcttcgtc
    12181 cagaatgaca tatacgtttt ccagagtgcg tccccgcata taagcaagcg gagcgatctc
    12241 gatcaaacct ttttcgatgt attcttcggt gagtctcaga ccgagcacct cgtacatggc
    12301 gtcatacaat ggtctgagat aaggatcgat cttttccttg agatcaccgg gaagaaagcc
    12361 caggttctcg ccagcttcga cagccggacg ggttaggacg attttcttga tcttgccttc
    12421 tttaagcttt cttagcccgt agagaacggc aagataggtt ttaccggtac cggccggtcc
    12481 gataccgaaa acgacggtgt ttttctcaat agcgtcaaga taggttttct gactaagcgt
    12541 tttggcgtaa accggttttc ccgaaaatgt tttcaccact tcctggcgac ccagaaaaag
    12601 ggaaagcaac tcttccttcc cggcgatatt gtacatgtga tggatataga tgatatcgcg
    12661 ctcgttgaac acgatgttgc gttttacaag ttgtaacaaa atatcgaaga ttcccctgat
    12721 ttcagaaatc ttctcttcgt catccgtatc gagtaaaata ccctcgtggt tgacgtagat
    12781 attgaatccg tataaaccgg ataaaatccg gacgtatttt tcgttgacgc cgaacagggc
    12841 gaccatttcg gaaacgttcg tcacgggtat gtccagcttg gtcagatcta ccatcggaat
    12901 cgcatcactc cctttatata attataccac acaacgggga aaaaaagcac ttcaacaatg
    12961 tgaagtgctc tcgagtcata cgcggtgtcc gcctttatgc tggcgctgtt tgagtttctt
    13021 ggaaacgctc ggtttcaaat aatattccct tttacgggct tcagccaagg ttccggcgcg
    13081 tgaaacgtct cgtttaaaac gacgcaatgc gtcttcgatg ctttcgttat ctcttacgac
    13141 ggtttttgcc atgtgctttc accccttttc ttcttgccaa aaaatattat acaggaatat
    13201 ttataaaaag taaacgattt ttttcgggaa attgatttca tttcttggcc agatatggta
    13261 ttatttcagg tgtatggaat cgaggtgttc gtatatggat ttgccaatgt tttattttat
    13321 gaatttgggg ttggtccaaa tcatcatcat cgctgtggca gcgatcgcat tcatcatcct
    13381 tttggcggtt ttcatcaata aaggacgcta tgcagcccgc tacaagaatt tctacaaaaa
    13441 gttggaccgc accatcaaca aaaacaagcg ctacaacggc aacat
//
LOCUS       DAOPJL010000030        13313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_4881_length_13313_cov_9.015236, whole genome shotgun sequence.
ACCESSION   DAOPJL010000030 DAOPJL010000000
VERSION     DAOPJL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_13313_cov_9.015236"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..1475
                     /gene="rsgA"
                     /locus_tag="PLP02_04660"
     CDS             591..1475
                     /gene="rsgA"
                     /locus_tag="PLP02_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPF42765.1"
                     /translation="MNASLPGKWRISEEINENIPVIGDWVMAKGENEQAVIREVLPRQ
                     SLFKRKTAGTTSLEQVIAANIDTVFICLSADGNFNLRRMERYLASVWSSGATPCIIIT
                     KADLTEKVETLLMEAKTVTFGTKVIVCSARSELGLSQLDDYLETGKTYVFVGSSGVGK
                     STMINHLVGFDIMETGSVRSDGKGRHTTTTRELFITISGAIVIDTPGMREFQLAEADF
                     DDAFPDIAELSADCRFRDCSHISEPGCAVREACENGELSEERLRNFFQMKKEIKYQEK
                     RTREKAVKFGKQTKNPNK"
     gene            1595..2311
                     /locus_tag="PLP02_04665"
     CDS             1595..2311
                     /locus_tag="PLP02_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPF42766.1"
                     /translation="MEKDQIETYFLDEKPRPLFLIFPGGAYSFTSWREGGPIARKYNE
                     IGMHAAVCKYRNEKLVYPDLVDNIEQMEKPLFNDPRVTKVFVVGFSAGGHLALQMMTR
                     HPEKYSGGILCYPVVMTQGEYCHQESISNLLGSNYSQESVKAINVIDHITKDMPPLFV
                     WHTEDDDTVPVENSLRLLKKAKEIGVKMELHIFPEGVHGLALATEETPFFGEDPEIFA
                     KKNKHVATWFNLMKEWLKTI"
     gene            2344..3444
                     /locus_tag="PLP02_04670"
     CDS             2344..3444
                     /locus_tag="PLP02_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42767.1"
                     /translation="MIAEVKTKKDLHRFIHFVEKLYKHDIHYVFPLFGPLGKELDEQV
                     LKTGKYKAILSLNFKGEAQGRMLYTYDFSKHAQKNVCYFSFFDAIDDQSVADELFEYM
                     ESDMRKDGVDFAEGTFAPYDPDTRRGILVKGFDTDPSLFTSYNYEYYGKLLENRGYVK
                     VYDTFTIKADVSPESERRLKALTDHFHRRFNVRIDSLDYKNLEKDLEDVHRIFQEATN
                     ELIYQDPPSMDMIRNVAENMRMFLDPDYIKIARENETERPVGFCLVLPDYNQVFKITK
                     GRIRPLKILFGKKKITRARGILQYLVPEYQGTGLIGSIYQSVYAKFRENHITEFVAGT
                     MMEKNPNAFHVFDKFGGRLHKIYRIYGKELTQ"
     gene            3441..4634
                     /locus_tag="PLP02_04675"
     CDS             3441..4634
                     /locus_tag="PLP02_04675"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPF42768.1"
                     /translation="MILPIIQVIAIFVIPYLIIRFHQFKLTKLLGTIGTAYLLGLLVA
                     LLVFILNELGIEFSLNEDVGEIGSHVAIGIAIPLLLFGANLREAKKLTRTVLLSFASL
                     LVSVTIVSILVFVFFGKNLEYGPELSAMAIGVYTGGTPNLNAIANIFRVDDTTIGIAN
                     LSDMVIGSVFYMFLLILCKPLLKKILPAPKGDSYLKEPTVNITNTDEFNFKEFKHTRS
                     LVRVFFLGLGIAAFGAGLGYLIWVIIDDPLINMVDIMVPTMMITVTILGIIASFNKRI
                     REVKGTNVVGQYLILVFSFALASSMDLSKMQTEFGKLLIVFGVVTIGGFVLHVFFSKL
                     LKIDADCTMVTATAGIYGPAFIPAVTKQIKNDELTIPGLICGSIGYALGTFLGLAIGY
                     LLMLF"
     gene            complement(4707..5255)
                     /locus_tag="PLP02_04680"
     CDS             complement(4707..5255)
                     /locus_tag="PLP02_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42769.1"
                     /translation="MKKVLMFAMTLALLLGLSGCALASKTKDYYATGNFNGWDTVESG
                     KMTAISKNDSRVATIADDLKEAEVLYLAEIVLPDTEAGWTVTYKINGTVTELDGNLTV
                     KVIRTTKDDPDTRDFWAQNPESGEITNLTPDTLYIPPFVEENVDQAGTWNDNPVAYAA
                     GTYYIVFAEFAETKAMALIPVE"
     gene            complement(5374..6966)
                     /locus_tag="PLP02_04685"
     CDS             complement(5374..6966)
                     /locus_tag="PLP02_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF42770.1"
                     /translation="MKKIVACLLVVLLSFAFISCSKEYVITDDLLETSSLVDDNNRIY
                     YEIYLGAYSDSNNDGIGDLQGLIGRLDYLNDGDQNSGKSLGIQGIWLMPIMPSPSYHK
                     YDVINYMEIDEDYGTLEDFSAFIEACDVRGIDVIIDLVLNHTSSQHSWFLAAKQALRD
                     GDLGNKYLDYYVLVTEDGKESGKTYYPFYGDYFYEGNFSSAMPELNLDSEYVREEIKT
                     IIAFWYDLGVHGFRLDAVKYAYLGDNEKNIEFWSWFAEECRKIEPNTYLVGEVWSADS
                     QIAPYYQCFSDFDFGMSQSDGAIASVANGVDDVNWYVNYINYYRDLVETYNTDAILTP
                     FISNHDMNRAAGYLSVSDGRMQMAANLYILTYGTPFIYYGEEIGMKGSRSTENTDANR
                     RLKMLWGDKDTVEDPIGATYSIDKQTNGTVKDQLKDETSLFNHYKKLIMLRNANPEIA
                     RGEYTPLVFNGYFSFGGFLSSYNGSTVGVFHNTSMSPITIDLSIYTDYDFSVVRGYVG
                     EGTATLSGQTLTIDGLTSVVLK"
     gene            complement(6991..7842)
                     /locus_tag="PLP02_04690"
     CDS             complement(6991..7842)
                     /locus_tag="PLP02_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF42771.1"
                     /translation="MERTSTVFSYILLVVLSIIWLYPIVWILLNAFRTEYNDQNQLIG
                     IVVSNYFPHQFGLENFRKLFVDTLFPRWFLNTLFVSSITFVLSTFLNLSVAYVMSKIK
                     FKARKPFLNIAIVLGLFPGFMSMIAIYYILKALGLTQTLFALILVYSAGAGLGFYVAK
                     GFFDLIPNSLIESARLDGATNAQIFNKIILPMSKPIIIYTALLAFTGPWMDFIFARVI
                     LGETNTHLHTVAVGLYYMMYGEHIDSNVFTTFAAGCVCVAIPIVTLFIAMQKYYVEGV
                     TAGAVKG"
     gene            complement(7896..9329)
                     /locus_tag="PLP02_04695"
     CDS             complement(7896..9329)
                     /locus_tag="PLP02_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014601612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF42772.1"
                     /translation="MQKKEENYLPKNKFLPSSNRLAKGKKNWFVTFGHRFADGSIGTK
                     ISHFIMGFGNFYHKQIIKGLLYFIIEAGFAFLMIFSPKVNDTPIGAKALVNLITLGTN
                     EGDIFTNADNSMLMLLFGVITWGIIFLFIFAYNSNLKSSYKSDLEVRKGKKPSSFKDD
                     LKDLLDGRFHVAMLTPSIIGIITFTVLPTIFMILIAFTNYDSLHLPPNNLFDWVGLDN
                     FIAIFSGSGNEISARFGSVLGWTIIWAFFATFTCYIGGILLALVINNKHIKAKKLWRT
                     IFVLTIALPQFISLLAVRNILSKEGPFNALLVFLGIIEQGNGIQFLGSADSILTAKIM
                     VIVINMWIGIPYTMLMTSGILMNVPAELYEAAQIDGATKRQAFYHITVPYILFVTSPY
                     LITSFVGNITSFNIIYLLTGGSPAVPGYIAGGTDLLVTWLYKLTIDQSEYSTGAVVSI
                     MTFIITATITLISYRRTKTYKEEDAFQ"
     gene            complement(9380..10711)
                     /locus_tag="PLP02_04700"
     CDS             complement(9380..10711)
                     /locus_tag="PLP02_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42773.1"
                     /translation="MKKVFMALFLCLFAVPVLAGCSGNTPLTIWVGSESVEFYTAKMA
                     EYVTEYNATHAEAFPYEISVLGVDTGTAASTYLDDTDAGADIFTVAHDNLGKLISGSS
                     SIAPITDVNLLAQIENDNPAIFLDVIKGTVDGVTYTFGVPYIAQSLVLYYNTDYITEE
                     QAQTWEGIWQAAIDNNKQALSLAGTDGYNNSFLLLARNAATNATTLRLYVDGVQSDCY
                     GTGDDTISVLKWGQRFFTDANGAKRPSDSGWETELKNGVSISFIGGAWHLAAAEAALG
                     SKLGVTVLPSFTITADDAYGTVAAGTVFQSGTFADTKMFVMKKNSKKANYLQDILLFL
                     SSSEVQEESFEECANLPAYKNALTEFAAFQENTIEAQLASAELAMFDYGIPQPFGFSS
                     KYNTYYYSKGAPDLILEILENNGGNFSTDAEIIAQMQIVESLWKTGARPTA"
     gene            10930..>13313
                     /gene="pulA"
                     /locus_tag="PLP02_04705"
     CDS             10930..>13313
                     /gene="pulA"
                     /locus_tag="PLP02_04705"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HPF42774.1"
                     /translation="MKKFIVALLTLIASTAFFAMVNAESGVATEDIYIHYFRYASDYD
                     NWGVWVWEVEPDDLEGETYSFTTDDTDSAYNFGGVVTHISISESFPGATKFGLIVKLG
                     NWEQKDIATDRKVEIPETTDNGELHIYLVEGDTRVGTSPDDPNGPTTNPKFLSAFFSE
                     LNEIYFKATETLSSDDIAVKANGIEVGINNIEIDGTTGTIYLVDEIDFSQKYTVEASF
                     PSDSSVNDYSVTFDGVYDSDEFVETFTYTGDDLGAYPSAEKTTFRIWAPISEQVVLNL
                     YTTGTPSNLGGTDTPYKTVTMTPDVKGTFYCEEAGNLHGVYYTYSVTNGTMTSEVVDP
                     YAKGVGINGVRGLVVDFSQTNPDGYTYDTRADNMENVTDAVIYELHVRDLTSDSTWNG
                     SEANRAKYLGLVESGTTYEGVSTGFDHITDLGVTHVQLLPFFDFGTLDESQVNDEDYQ
                     AFNWGYMPINYNALEGSYSSDPYDGLVRITEMKEVVMAFTAADIRVNMDVVYNHSGLT
                     ADSNFELIVPGYYFRKTAAGEFSNGSGCGNETASERSMMRKFIIESCVFWATEYNLSG
                     FRFDLMALEDIDTMNMVADALHEIDPTIMVYGEPWTGGTTVLSESLKSGKSNLYELPT
                     IAAFNDDFRDAVKGTIWIGEQGGYVQGYFTAQIMNRLKYGIVGGIAYPGIDVSQLSVF
                     KAWHTSPTKTINYVTCHDNNTLYDKLYMTLEESKDLDLISTLDKQAYALVMTSQGITF
                     IHAGDEILRSKPLDDGSGFDSNSYTSPDSVNSIKWDQKADETGYGVFEYYQGLIA"
BASE COUNT         3852 a         2718 c         3092 g         3651 t
ORIGIN      
        1 aacatgtacg tatgatttgc cagcatgaca taaggctgtt ttctatgaaa attgaccgaa
       61 ggatcgcgat gtaccaccct ttttgcgtcc agccacatcc aaagaaagac caaaggtctc
      121 aaaataaaca aaaggaaatc ctgttgatgg tcgatccggg tttttttcat atgtcctccc
      181 tttgttgcat ttgatcttat tataatacat tttaagccat ccgtaggtgt tttttggcga
      241 aaaaacgcca attggtcata ttaaaggttg tatattaaaa gcaatttatt atataatata
      301 tttatccggc aacacctgat tttccggaaa tcattttttg aaaggggaaa tgtatgatga
      361 cggtagaata tgaagaagcc attcaaaaag tgacggaggg cactggcaag taaacaacta
      421 accctccaaa caaataataa ttggaggata tgatggaata taataattta agccggtacg
      481 gactcaacga tcgtattcgg catctagcat cgtcatacga cgggcttttt ctcgcacgcg
      541 ttgttgcgac acaacgaaat aattttcagc ttatgtcaga aaccggtgaa atgaacgcga
      601 gtctgccggg taaatggaga ataagcgaag agattaatga aaatatcccg gtaataggag
      661 actgggtgat ggccaaagga gaaaacgaac aagcggttat cagggaagtt ttaccaaggc
      721 aaagcttatt taagcgaaaa acagccggga cgacatccct agaacaggtg atagcggcca
      781 acatcgatac agttttcatt tgcctttcgg cagatgggaa tttcaatttg cgaagaatgg
      841 aaagatatct ggcgtcggtt tggagttctg gcgccactcc ttgcatcatc attacaaaag
      901 ccgatttgac agaaaaagtg gaaactttgc taatggaggc aaaaacagtg actttcggca
      961 caaaggtcat tgtctgttcg gcaagaagcg agttgggcct ttcccaactc gacgattact
     1021 tagaaacagg caaaacctac gtgttcgtag gatcttccgg tgtcggtaaa tcgacaatga
     1081 tcaatcattt ggtaggcttt gatatcatgg aaaccggttc ggtaagaagc gacggaaaag
     1141 gaagacacac cacaacgaca agggaactgt tcataaccat ttccggagcg atagtgatag
     1201 ataccccggg aatgcgggaa ttccagctgg cggaagctga ttttgatgat gctttcccgg
     1261 atattgcgga attgtcggcc gattgccgtt ttcgcgattg ttcccatatc tcggaaccgg
     1321 gatgcgccgt tagagaggct tgcgaaaacg gagagctttc agaagaaaga ttaagaaatt
     1381 tctttcagat gaaaaaagaa ataaaatatc aggaaaaaag aaccagagaa aaagctgtaa
     1441 agttcggaaa acagacaaaa aatccgaata aataagggaa tgcaatggga aaatagcgca
     1501 cgaaagagtg cgattttggt cgttgttttc cccttttttt cttttataag aaagagatgg
     1561 tgtataataa gtactaagtg agattggaga aaagatggaa aaagatcaaa tcgaaacgta
     1621 ttttcttgat gaaaagccaa ggcctctttt tttgattttt ccaggaggtg cttacagttt
     1681 cacatcctgg cgagaaggcg gaccgatagc tagaaagtat aatgaaatcg gtatgcacgc
     1741 ggccgtatgc aaatatcgga acgaaaaact ggtctatccg gatttggtag ataacatcga
     1801 acaaatggag aagccgttgt ttaatgatcc gcgagtgaca aaagtgttcg tagttggatt
     1861 ttctgccggc ggacatctag cgctccaaat gatgacaaga catccggaaa aatactctgg
     1921 cggtatcctt tgttatccgg tggttatgac gcaaggggaa tattgtcacc aggaatcgat
     1981 atccaatctt ttgggtagca attattccca agaaagcgta aaagccataa atgtcattga
     2041 tcatattaca aaggatatgc caccgctttt tgtatggcat acggaagacg acgacactgt
     2101 cccggtggaa aactcgctta ggctattaaa aaaggcaaaa gaaatcggtg tcaagatgga
     2161 attgcatatc tttcccgaag gagtacatgg actcgcactc gctaccgagg aaacaccgtt
     2221 ttttggtgaa gatccggaaa tttttgccaa aaagaataag catgttgcca cctggttcaa
     2281 tctgatgaaa gaatggttaa aaacgattta gaaaaattac gagaatattt tgcggaggtc
     2341 gagatgatag cagaagtaaa gaccaaaaaa gacttgcatc gtttcattca ttttgttgaa
     2401 aaactctata agcacgatat ccattatgtt tttccgcttt tcggcccttt ggggaaagaa
     2461 ctcgacgagc aggttctgaa aaccggaaaa tacaaagcca tcctgtcttt gaattttaaa
     2521 ggcgaagcac aaggacgaat gctttacact tatgatttca gcaagcatgc ccagaaaaac
     2581 gtatgttact tttcgttttt tgatgccatc gatgatcaga gcgtggcgga cgaactgttc
     2641 gaatatatgg aatccgatat gagaaaagac ggagtggatt ttgccgaagg gacattcgct
     2701 ccttatgatc cggatacgag aagaggcatt cttgtcaaag ggtttgacac cgacccgtct
     2761 ctttttacct catataatta tgaatattac ggtaagcttc tggaaaatcg aggatatgtt
     2821 aaagtatatg acactttcac cataaaagcg gatgtgtcgc cggaaagcga gagacggttg
     2881 aaggctttaa cagaccattt tcatcgccgt ttcaatgtca ggatcgattc tctcgattat
     2941 aaaaacttgg aaaaagattt agaagatgtc caccgaatat tccaggaagc gaccaacgaa
     3001 ctcatttatc aagaccctcc ttcgatggac atgatcagaa atgttgccga aaacatgcgg
     3061 atgttcttag atcccgatta catcaagata gcaagggaaa acgaaacgga aagaccagtt
     3121 ggattctgtc ttgtcttgcc tgattacaac caagttttca aaataaccaa aggccgaatc
     3181 cgtccgctaa aaatattatt tggcaaaaag aaaatcacga gagccagagg catactgcaa
     3241 taccttgtgc cagagtatca gggaactgga ttgatcggga gcatttacca atctgtgtat
     3301 gcgaaattcc gtgaaaacca cataacagaa tttgtggccg gcacgatgat ggaaaagaac
     3361 ccaaatgcct ttcatgtgtt tgataaattc ggcgggcgac ttcataaaat ataccgcatc
     3421 tacggaaagg aactaacaca atgattctgc caatcattca agtaattgcc atctttgtca
     3481 taccctacct gatcatccgt ttccatcagt tcaagctgac aaaattgttg ggtacaatcg
     3541 gaaccgcata tcttttgggc ttattggttg ctttattggt ttttattctg aatgaactcg
     3601 gtatcgaatt cagtctgaac gaggatgttg gagaaattgg cagccatgtc gcgatcggca
     3661 tcgccatacc gcttttgttg ttcggagcga atcttcgcga agcgaaaaaa ctgacaagaa
     3721 cagttttgtt gtcatttgcc tcgcttttgg tatcggtcac cattgtttcg attctcgtat
     3781 ttgtcttttt tggaaagaat ttagaatatg gcccggaatt gtccgcgatg gctatcggag
     3841 tctatactgg tggtacgccg aaccttaatg ccattgccaa catattcaga gttgacgata
     3901 caacgatagg catagccaat ctttccgata tggtaatcgg cagcgtattt tacatgttcc
     3961 ttctgatttt atgcaagcct ttgctcaaga aaattctccc ggccccgaag ggagattcgt
     4021 atctgaaaga gccgacagta aatatcacca atacggatga gttcaatttc aaagaattca
     4081 aacacacccg gtcattggtc cgagtgtttt tcctgggcct gggaattgcc gcgttcggag
     4141 ccggactcgg atatcttatt tgggtaataa ttgacgatcc tttaataaat atggtagata
     4201 taatggttcc aacgatgatg atcacggtca cgatactagg tataatcgct tcgttcaaca
     4261 aaagaatcag agaagtgaag ggaacgaatg ttgtcggtca atacctgatt ttagttttct
     4321 cctttgcttt ggcatcatcc atggatttgt cgaaaatgca aactgaattt ggaaagttgc
     4381 tcatcgtatt cggcgttgtg acaataggcg gatttgtatt gcatgtattc ttttccaagc
     4441 tgttgaagat agatgccgat tgcacgatgg taacggcaac ggctggtata tacggtccgg
     4501 cattcatacc ggcagtcaca aagcagatca agaatgacga actgacgatt cccggattga
     4561 tttgcggatc gatagggtat gctttgggaa cttttttagg gcttgcgatc gggtatttac
     4621 tgatgttgtt ctaaacagaa taaggtaata aaaaaacgac ttcaggcagt ttgccaaaag
     4681 tcgtttcttt ttttgttctg gtctgcttat tcgacaggga tcaaagccat tgcttttgtt
     4741 tctgcgaatt cagcaaatac gatgtaataa gttccggcgg cataagcgac tgggttgtcg
     4801 ttccatgttc cggcctggtc aacattctct tctacaaacg gcggaatgta gagggtgtcg
     4861 ggtgtcaagt ttgtgatctc gccgctttcc gggttctggg cccagaaatc tctggtgtcg
     4921 ggatcatctt ttgtggtacg aataacctta acggtcaagt tgccatcaag ctcggtaact
     4981 gttccgttga tcttgtaggt tacagtccag ccagcttctg tatcgggaag aacgatttcc
     5041 gcgagataga gaacctcggc ttctttaaga tcgtcggcta ttgttgcgac tcttgaatca
     5101 tttttgctga tagctgtcat tttgccactt tcaacggtat cccagccgtt gaagtttcca
     5161 gtagcataat aatctttggt cttgctggcc aatgcgcatc cgctaaggcc taacaataaa
     5221 gccaaagtca tagcaaacat taaaactttt ttcatacact tctctccttt tttcttattt
     5281 ttgtttcttt tagcttacgt ttattttatc attttagtac gtaagtgtca ataaagcaac
     5341 catggtatcc aataaaagtg aaaccgctac cacttatttg agaacaacgc ttgtcaaacc
     5401 gtcaattgta agagtctgac ctgataacgt ggcggttcct tctccgacat agcctctcac
     5461 gaccgagaaa tcataatcgg tataaatact caagtcgatg gtgataggcg acatgcttgt
     5521 gttatgaaac acaccgacag tggaaccgtt gtatgaagag aggaatccgc caaaactgaa
     5581 gtagccgtta aaaaccagcg gagtgtattc gccccgggcg atttccgggt tggcgtttct
     5641 caacatgatc agctttttgt aatgattaaa cagacttgtt tcgtctttca gttgatcttt
     5701 tacagtgcca ttggtttgtt tgtcaatcga gtaagtggcg ccgatcggat cttcgacagt
     5761 atccttgtcg ccccataaca ttttcaaacg gcgattggcg tccgtgtttt ctgtcgatct
     5821 cgatcctttc atgccgattt cttccccgta gtaaatgaac ggagtaccgt atgtaagaat
     5881 ataaagattg gcagccattt gcatgcgtcc atcggaaacc gaaagatatc cggccgcacg
     5941 gttcatgtcg tgattggata tgaaaggtgt aagaatagcg tccgtattgt aagtttccac
     6001 caaatcccgg taatagttta tataattcac ataccagttg acatcatcca caccatttgc
     6061 aacggaagcg atcgcgccat cggattgaga catgccgaaa tcgaaatcgc tgaaacattg
     6121 atagtaaggc gcgatctgcg aatcggcaga ccacacctcg cctaccagat aggtattcgg
     6181 ttcaatctta cggcattctt ccgcaaacca cgaccagaat tcgatgtttt tttcattatc
     6241 gcccaaatag gcgtatttca cggcgtcgag acggaaaccg tggacgccaa gatcatacca
     6301 gaaagcaatg attgttttaa tttcttccct gacatattct gaatcaaggt ttagttcggg
     6361 catggcggag gaaaaatttc cttcatagaa ataatcgcca tagaaaggat aataagtctt
     6421 cccgctttcc ttcccgtctt cggtgaccag gacatagtag tcaagatatt tattgccaag
     6481 atcgccgtct ctcaaggctt gcttcgctgc caagaaccag gagtgctgac ttgaagtatg
     6541 attcaacacg agatcgatga taacatcaat ccctctgaca tcacaagctt cgataaaagc
     6601 cgagaaatct tccagagttc cgtaatcctc atcaatttcc atataattga tgacatcgta
     6661 tttatgatag cttggggatg gcatgatcgg cataagccag atgccttgaa tacccaaact
     6721 ttttccggaa ttttgatcgc cgtcgtttaa ataatccaat cggccgatta gaccctggag
     6781 gtctccgatt ccgtcattgt tggaatcgga gtacgctcca agatatattt cataataaat
     6841 ccgattgtta tcgtcgacca aagaagaagt ttcgagaaga tcatcggtga ttacatattc
     6901 ttttgaacac gaaatgaaag caaaagacaa aaggacaaca agaaggcagg caacgatctt
     6961 tttcatattg acagcctcct ttgacgggaa ttatcccttc acggctccgg cagttacgcc
     7021 ttcgacgtag tatttttgca tggcgatgaa cagagtgacg atcggaatgg ccacgcaaac
     7081 gcacccggcc gcaaaggttg tgaatacatt ggaatcaatg tgttctccgt acatcatgta
     7141 ataaagaccg acagcgacgg tgtgcaaatg ggtatttgtt tcacccaaaa tgactctcgc
     7201 aaagatgaag tccatccatg gccccgtgaa agcgagcaaa gccgtatata tgatgatcgg
     7261 cttactcatc gggagaatga ttttgttaaa aatctgagca tttgtcgcgc catccagtcg
     7321 cgccgattca atcaaagagt tgggaatcag atcgaagaac cctttggcga catagaaacc
     7381 cagaccggct cccgccgaat acaccaggat tagagcaaat aaagtttggg tgagcccgag
     7441 agctttcaga atgtaataaa tggcgatcat cgacatgaat cccggaaaaa gacccaaaac
     7501 gatggcgatg ttcaagaaag gttttcgagc tttgaatttt attttgctca ttacgtacgc
     7561 tacgcttaaa ttgaggaacg ttgataaaac gaaagtgatt gaagatacga acaaagtatt
     7621 caaaaaccat ctcggaaaca gggtgtcgac aaagagcttc cggaagttct caagaccgaa
     7681 ttgatggggg aaataattgc ttacgacgat tccgatcaat tggttttggt cattgtattc
     7741 ggttctgaac gcgtttagca atatccaaac aatcggatac agccatatga tggacaacac
     7801 gacgagcagg atgtaggaaa atactgttga cgtgcgctcc atcgttttat aactcttgaa
     7861 ttttttggga gaatcgactc cattgatatc tttcatcatt ggaaagcatc ctcctcttta
     7921 taagtttttg ttcttcgata cgatatcaaa gtgatcgtgg cggtgatgat gaatgtcatg
     7981 atcgatacca cggcgccggt actgtattcg ctttggtcaa tggtcagttt gtagagccag
     8041 gtgaccaaga gatcggttcc gcccgcaata tatccgggca ccgccggaga accgccggtt
     8101 aatagataaa tgatgttgaa acttgtgata ttgccaacga acgaagtaat tagatacggc
     8161 gaggtcacaa acagaatata aggcaccgtg atatggtaaa acgcctgacg tttggttgcg
     8221 ccgtcgattt gtgccgcttc ataaagttcg gcggggacgt tcatcagaat accgcttgtc
     8281 attaacattg tgtacggaat accgatccac atgttgatga cgatgaccat gatttttgct
     8341 gtcagaatac tgtcggcgct tcccaggaac tggattccgt taccttgttc gatgattccc
     8401 aaaaaaacca acaaagcatt gaacgggcct tctttggaaa gaatgttacg aaccgccagc
     8461 aaggaaataa attgtggtaa agcgatggtc agaacgaaaa tcgtgcgcca tagcttcttg
     8521 gctttgatat gcttgttgtt aatgacaaga gccaagagaa ttccgccgat ataacatgtg
     8581 aaggtggcga aaaacgccca aatgatagtc cacccgagaa ccgagccgaa ccgagccgat
     8641 atttcgtttc cggatccaga aaagatagca ataaagttat ccaggccaac ccaatcgaac
     8701 agattgtttg gcggtaaatg cagtgagtcg taattagtga acgcaatcag gatcatgaag
     8761 atcgtcggca aaaccgtgaa agtgatgatg ccaatgattg acggagtcaa catggctaca
     8821 tggaaccggc cgtcgagaag atctttcaga tcgtccttga atgaagaagg tttcttgcct
     8881 ttccttacct cgaggtcaga tttgtaggaa cttttcaagt tggaattata ggcaaagatg
     8941 aataggaaaa tgatacccca ggttatgacg ccgaacagaa gcatcagcat cgaattgtcg
     9001 gcgtttgtga aaatgtcgcc ttcgttcgtg cccaatgtga tcaaattaac gagagctttg
     9061 gcgccgatgg gggtgtcgtt cactttcggt gagaatatca tcagaaaggc aaagcccgcc
     9121 tcgatgatga aataaagcaa tcctttgatg atctgcttgt gatagaaatt gccgaatccc
     9181 atgatgaaat gggatatttt ggtgcctatg ctaccgtccg caaaccgatg cccgaaggtg
     9241 acgaaccaat tctttttgcc ttttgccaac cggttggaag agggaagaaa cttattcttt
     9301 ggcaaataat tttcttcttt tttttgcatg agttctcctt ccgattataa tatttggttt
     9361 ttaagcgaaa aggtatctgt tatgcagtgg gacgcgcgcc ggtcttccag agcgattcca
     9421 caatttgcat ttgggcaatg atttcggcgt ctgtagagaa gttgccaccg ttgttttcca
     9481 agatttcgag aatcaggtca ggagcgcctt tggaataata gtaagtgttg tatttgctgg
     9541 aaaaaccgaa aggctgagga atgccgtagt cgaacatcgc gagttccgca ctggccaact
     9601 gagcttcaat tgtattttcc tggaatgcgg cgaattcggt caaagcattt ttataagccg
     9661 gaagattggc gcattcttca aatgattctt cttgaacttc ggaactggac aagaacagaa
     9721 ggatgtcttg gagataattg gctttcttgc tgttcttttt cataacgaac atttttgtat
     9781 ctgcaaaagt accggattgg aatacagtgc cggctgcaac ggttccataa gcgtcatcag
     9841 cggtaattgt gaaagacgga agaacagtca cgccgagttt gcttccaaga gcagcttcgg
     9901 cagcggcaag atgccaggcg ccgccgataa acgagattga gactccgttt ttaagttcgg
     9961 tttcccaacc ggaatcggaa ggtctcttgg ctccatttgc atcggtgaag aatctttggc
    10021 cccattttaa aacggaaatg gtgtcatcgc ccgtgccgta gcaatcgctt tggacgccgt
    10081 cgacataaag tcttagagtc gttgcgttag ttgcggcgtt tctggccaag agtaggaatg
    10141 agttgttgta tccgtcggtg ccggcaagcg acaacgcttg cttattgttg tcaatggcgg
    10201 cttgccaaat tccttcccat gtctgggcct gttcctcggt tatataatcg gtgttgtaat
    10261 aaagcacaag gctttgggcg atgtaaggaa caccgaaagt ataagtgaca ccatcaacgg
    10321 ttcctttgat gacatcgagg aaaattgccg gattatcgtt ttcaatctgc gcaagcaggt
    10381 tgacatcagt gatcggggcg atactgctag agccgctgat cagttttccg aggttgtcat
    10441 gtgccacagt gaaaatgtca gccccggcgt ctgtgtcatc cagataagtt gatgccgcgg
    10501 ttccggtgtc aactcccagg acactaattt cgtacggaaa agcttctgcg tgagtagcat
    10561 tgtactcagt tacgtattct gccatttttg ccgtataaaa ttccacggat tcggacccaa
    10621 cccaaattgt caagggggtg tttcccgaac atcccgctaa tacaggaacg gcaaacaggc
    10681 acaagaacag tgccataaac acttttttca tttgttttct ctcctttttt tatgaataac
    10741 attgtaaaat aattttagca tctatataat tctaatgcaa gggcttacaa ggatgttgaa
    10801 ttatgtgaaa ccggtttttt cttgtgatac cgtttacaac tttttcttaa tatttacaca
    10861 tatttgcatt tgtcgctgat ttgacctata tttaatgtag aatgtatatt tttcgcaagg
    10921 gggataaata tgaagaaatt tatagtagcg ttgctgacac ttatcgcatc aaccgctttt
    10981 tttgctatgg tgaatgcaga aagcggagtg gcaaccgagg atatttatat tcattatttc
    11041 aggtatgctt cagattatga caactggggt gtgtgggtgt gggaagtcga acccgacgac
    11101 cttgaaggag agacatattc atttactacc gatgacaccg acagcgcata caattttggc
    11161 ggtgtggtta cgcacatatc aatttcagag agttttcccg gcgcgacaaa atttggactt
    11221 atagtcaaac ttgggaattg ggaacagaag gatatcgcta ccgaccgcaa ggttgaaatt
    11281 ccggaaacaa cagacaatgg tgaattgcat atttatttgg tcgagggcga cacgagagtc
    11341 ggaacatcgc ccgacgatcc gaatggaccg actacaaatc ctaaattctt gtctgccttt
    11401 tttagcgagt tgaatgaaat ttattttaaa gcaaccgaaa ccttgtcttc tgacgatatt
    11461 gccgttaaag ccaacggtat cgaggttggt atcaacaata tcgaaatcga cggaactacc
    11521 ggcacgattt atctcgtgga tgaaatagat ttttcccaga aatatacggt tgaagctagt
    11581 tttccttcag attcgtcggt aaatgattat tctgtcacat ttgatggcgt ttatgattct
    11641 gatgaatttg tagaaacctt cacttatacg ggagatgatc tgggagcata tccaagcgct
    11701 gaaaaaacaa ccttccggat atgggctccg atttccgaac aggtagtttt gaatttgtat
    11761 accacgggaa cgccgtccaa tctgggcggt accgatacgc cgtataaaac agtcactatg
    11821 acgcctgatg taaaaggcac attctattgt gaagaagccg gaaatcttca cggcgtttat
    11881 tacacatatt ccgtcactaa cggaactatg acaagcgaag tggtcgaccc gtatgccaaa
    11941 ggcgtcggca tcaatggagt caggggactg gttgtagatt tttctcagac aaacccggac
    12001 ggatacacat acgatacaag ggccgacaat atggaaaatg ttacggatgc cgtcatttat
    12061 gaactgcatg ttcgcgactt gaccagcgat tccacctgga acggcagcga agcgaaccga
    12121 gccaaatatc tgggcttggt tgaatccggc acgacatacg aaggagtatc gaccggattt
    12181 gatcatatta ccgatctcgg agtcacgcac gtccagttgt tgccattctt cgattttgga
    12241 acattggacg aaagtcaagt gaacgatgaa gattatcaag ctttcaactg gggatacatg
    12301 cctataaatt acaacgcttt ggaaggatcg tattcatcgg atccttatga cgggcttgta
    12361 cgtattaccg aaatgaaaga agtcgtaatg gcttttaccg ccgccgatat cagggtgaat
    12421 atggatgtcg tatataatca cagcggtttg actgccgatt ccaattttga gctgatagtt
    12481 ccgggatatt atttccggaa aacagctgcc ggagaattca gcaacggatc cggctgcggg
    12541 aacgaaacag catccgaacg atcgatgatg cgcaaattca tcatcgagtc gtgtgttttc
    12601 tgggctaccg aatacaattt atcaggattc agatttgatc tcatggcttt ggaagacatc
    12661 gacaccatga atatggtggc ggatgctttg catgaaattg atccgacgat catggtttac
    12721 ggcgaaccgt ggaccggagg aacaactgtt ctttccgaat ccttgaaatc gggtaaatca
    12781 aatctgtatg aattgccgac catagcggca ttcaacgatg atttccgcga tgcggtcaaa
    12841 ggaacgatct ggattggcga acaaggcgga tatgttcagg gatattttac cgctcagata
    12901 atgaacaggc tgaaatacgg aatcgttggt ggcatagctt atccgggaat cgatgtatcg
    12961 caattgtctg ttttcaaagc gtggcataca tcgcctacaa aaacgatcaa ttacgtgacg
    13021 tgtcacgaca acaatacctt atacgacaag ttatatatga cactcgagga aagcaaagat
    13081 cttgatctca tatccacgct tgacaagcag gcgtatgctt tggtcatgac ttcccaggga
    13141 ataacattca ttcatgccgg tgacgaaata ctgcgttcga agccgcttga cgatggaagc
    13201 ggattcgaca gcaactcgta cacgtccccg gattcggtca actccataaa atgggatcag
    13261 aaagccgatg aaaccgggta tggcgttttt gagtattacc aaggtttgat tgc
//
LOCUS       DAOPJL010000031        12861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_5132_length_12861_cov_5.135874, whole genome shotgun sequence.
ACCESSION   DAOPJL010000031 DAOPJL010000000
VERSION     DAOPJL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12861
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_12861_cov_5.135874"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLP02_04710"
     CDS             <1..790
                     /locus_tag="PLP02_04710"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346601.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPF42775.1"
                     /translation="ICEIAKLVHSAGGLMYYDGANLNAILGKARPGDMGFDIMHINLH
                     KTFSTPHGGGGPGSGPVGVRKGLEYLLPNPKVDIMDGHYYIKGDADSIGRISMHFGNF
                     LVFVKAYAYILSLGNENIGKVGELSVLNANYVMESLKDDYKLPIPLHCKHECVFDGLK
                     DDSTGIKTLDVAKRLLDYGMHAPTIYFPLLFHQSIMIEPTETESKQTLDDFIMAMKSI
                     AREAKEDPELLHQPPHTTPVRRIDEVYAVRKMIITYKDYCENKE"
     gene            806..1525
                     /locus_tag="PLP02_04715"
     CDS             806..1525
                     /locus_tag="PLP02_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPF42776.1"
                     /translation="MYPQYFSIIDIVIVLGVIAFGVIGWKKGFLLTIIKMASSVFGLI
                     ASLLLSKSFAKVLDGWFGASVDTKIYEYMMSRSDLFSSSLSVDNVRTALEGMSMPTFL
                     IDWIISKIDFSQIGVSIVDAIEPTIKSIVMVVLAFIILFFGSMLVFLILKMIAKLITS
                     IPFIKQVDKGFGVLFGVFKMAVVTYVLLFLLALLMAVPVVTNLIGDFVTSDMQLSTDA
                     FRLSKWLYNNNVIQDFIGIFF"
     gene            1634..2227
                     /locus_tag="PLP02_04720"
     CDS             1634..2227
                     /locus_tag="PLP02_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPF42777.1"
                     /translation="MKYIEYLNQRHSVYALSNEEVIPDDDITKLVGSVIEATPSAFNS
                     QTQRVMILFGPRHLEFWKILMNELQNIVPPESFPRTEAKINGFAAARGTILFFDSLDE
                     TEKLMEQFPLYKKNFALWSQQQNGMLQENVWVALATEGIGASLQHYNEIVKGKIEAAF
                     GIPSGWDIIAQMPFGKIMEKPEAKPKLKLETRLIVRE"
     gene            2310..3251
                     /locus_tag="PLP02_04725"
     CDS             2310..3251
                     /locus_tag="PLP02_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42778.1"
                     /translation="MKLVEWLSNGDPVIKKLVERHLLDHDVLNKDEGYIHRYLVLFDW
                     KRNRWSGSVYSPKWISTHYTMMELKYMEVPPDNPEYTSGVETLISGMWEKPGMYGKYP
                     DQDLCVVAMILGMASYVKFRDERLHDMVDYILAHCFPDGGWNCNWNSVTHPASKSSLH
                     TTISVLEAFRDYLKNGYEYRREEVLTSIFSAEDFVLRKHLFRSERTGEIIHKAMIECH
                     YPERWHYDLFRGLEYFQSVQKPYEERMNEALDIVEAKFGRKGYLTKGPFYPGLVHFDL
                     ETSRAGRFATLKALKILKFYRRSVFDILMDAEIGDPK"
     gene            complement(3272..4333)
                     /gene="ispG"
                     /locus_tag="PLP02_04730"
                     /gene_synonym="gcpE"
     CDS             complement(3272..4333)
                     /gene="ispG"
                     /locus_tag="PLP02_04730"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPF42779.1"
                     /translation="MYLRTETKTIFVGNLPIGGNNHVYVQSMTTTKTKDIDATIEQIK
                     RLENVGCELVRVAIRDHDDAAAVAVIKQNISIPLIADIHFDYRLALEAIENNIDKIRI
                     NPGNIGSSENIRKVVEACGKKGVPIRIGINSGSLEKDLLETYKDRKAEAMVESAKRQV
                     ALLEQLGFTAIFLSLKSSDLATTVRANQLAAEIFRYPLHLGITEAGTAFSGAIRSAIG
                     LGIMLGKGIGATIRVSLTADPVDEIKAGKEILSDLSLYDMPILISCPTCGRIQYDMFP
                     IANAIEDYLQTVHKKIKVAIMGCAVNGPGEAKDANVAICGGKDEALLFIDGVQVKKIP
                     QSDLIPELKKAIALYKEKK"
     gene            4454..6067
                     /gene="ptsP"
                     /locus_tag="PLP02_04735"
     CDS             4454..6067
                     /gene="ptsP"
                     /locus_tag="PLP02_04735"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPF42780.1"
                     /translation="MKIIRGVGASGQWAAGRVVILKKESNMVESESKTTHSEEKERFF
                     KARQKAESELDRLIEKLGKSDQASSQIFASHREMINDVILVDRVKEMIDRGESAEMAV
                     TEACDHFYGLLSRAEDEYLRQRKDDVLDVKNRLMQALSQKKADEAIPDGSYIVFSERL
                     FPSDIMNYSSRGANGFVSRVGSVYSHTSILARNMGIPYVCQLDATYETIKESEYVVID
                     GKSGEVYLGLDEKTAANILASRNKAIEKTMEDALSVTAERQEASLKIKIMANANRLED
                     IDLIKKTKASGIGLVRSEYLFAENEKYPNEEEQEIVYERFIKALAPLPVTIRTFDFSS
                     DKPNRFRRYEKEENPALGYRGIRISLEEPENFKIQLRALLRASKAGNLRILFPMITGL
                     SQVMAIKKILLDAKKELRSESKEIADNIEIGLMIETPAAALVADILAPEVDFFSLGTN
                     DLIQYTLATDRQNFRTAGLYDENHPAVRKLMQMTCKAAAKARIPVAICGEMAENLHLS
                     NLFLTLGINELSVSSGKVDKIRKAYAVTE"
     gene            complement(6114..6560)
                     /locus_tag="PLP02_04740"
     CDS             complement(6114..6560)
                     /locus_tag="PLP02_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42781.1"
                     /translation="MKKFSLTTLFQKLASLLIDAFFIAFYLILFIEGDSFSVILLVFG
                     ILTLILCITYTAIILHAKVIVDRARDVVICRIVKSTIYDIRHITNAKIEERLDGHKKK
                     NVIALYNELGYQIGEINPYLLKKTLPSLPKICDEINGAVASLQKIM"
     gene            6699..7973
                     /gene="eam"
                     /locus_tag="PLP02_04745"
     CDS             6699..7973
                     /gene="eam"
                     /locus_tag="PLP02_04745"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular transferase
                     activity, transferring amino groups [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HPF42782.1"
                     /translation="MGKSKRQVALDRARELKTRIADYLEIRPTIPKGLEKKAEIGERQ
                     RRIRETLHATTENWHDWHWQLKNRVKDVGSLKRFLKLSDRESEAIAKVSETFRWAISP
                     YYLSLIDPNSDLDPIKLMAVPSVLEIGDREHGLDPMKEEYTNPAGCITRRYPDRLIIN
                     TTNECAMYCRHCQRRRNIGEKDAARSQKEIAESITYIKDNPEIRDVLLTGGDPFTLSN
                     QRLEWIISELRKIKHVEIIRIGTRTPVTMPQRITDKLVAMLKKYHPLYINTHFNHPME
                     VTEEAKEACAKLADAGIPLGNQAVLLNGINNDPYVMRCLNQELLKIRVKPYYIFHAKT
                     VRGTTHFNTSVDDGIEIMEYLRGYTSGLAIPSYIVNAPGGKGKTPILPEYLISRGSDY
                     FVIRTWEGEIVRHENHPSKDLKKMLEEKYQTF"
     gene            8031..8690
                     /locus_tag="PLP02_04750"
     CDS             8031..8690
                     /locus_tag="PLP02_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPF42783.1"
                     /translation="MCGRFTLGVTASKLIKYLKENFGITCSPETFDVPRFNVSPGQDI
                     VTIIAAKEGFHSGYLNWSYRFSTSGRGLVNIRAESLEEKTTFLKSFQQRRCLVIADGF
                     YEWKTTDGKKKAYWIRPADEGIFLMAGLWISQGEGKEKRFYAAIVTHPANSDMEGIHH
                     RMPVIFTPEDGRKWLDRQAGEEKLLDLIRIYPEKLTIEPVSNRVNDPDIDDERCIWRE
                     D"
     gene            8693..9634
                     /locus_tag="PLP02_04755"
     CDS             8693..9634
                     /locus_tag="PLP02_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42784.1"
                     /translation="MSYIIIRETKEKYLPDLMNMWNDGEVMKYVNYPNGLGMYGFKME
                     SWYKWLQSQGDTKHFIILDDETGFLGETFYCYAKKNKPASLDIKLIKNARGRHVGNLA
                     LSYAIDMLFKNTKAMTAYVDPNKENLPALSLYKRLGFEKVEKQNDLYPDAVYMEINRE
                     KWQKQRLESIRLAPIDRNNVFPVCFLKVKDDQKNFVATNSLSLAQAAYQTECIPHAIY
                     CGYNLVGFLMHATDEKDGLQWIYRLMIDRDFQGLGYGEKAMKIVMDTLKKQSKSNRIR
                     ISFEPANKIARQLYEKLGFVDSGQMDGDETIYEYTWQ"
     gene            9650..9865
                     /locus_tag="PLP02_04760"
     CDS             9650..9865
                     /locus_tag="PLP02_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HPF42785.1"
                     /translation="MNNWLTMDPYIVLSLANMKLRNFYPDLDALCDDIGVDRRALEDK
                     LLIIGYHYERKGNHFVAAKDKVTMEEK"
     gene            9862..10464
                     /locus_tag="PLP02_04765"
     CDS             9862..10464
                     /locus_tag="PLP02_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HPF42786.1"
                     /translation="MKIRYEGNGTIAKILMDTDPILVPIFVKTPFVEFETGSDYFRHL
                     AGAIVTQQLSSRVADAIFRRLLEYTKNDLSPEKILVSPVDDLRSQGLSYRKAEYLKLL
                     ATRFVEDPGLFKSLSEKTDEEVTSILCEIKGIGQWTAEMFLMFTLGREDVFSAGDYGL
                     KRALSMLYNRPVNEKEATAMAKKWQPYRSVVSHFLWHYLD"
     gene            10578..12782
                     /locus_tag="PLP02_04770"
     CDS             10578..12782
                     /locus_tag="PLP02_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPF42787.1"
                     /translation="MKSLKNKIILAVFLVLTIVAGIMITRINVNYDLKQYLPDDSETI
                     EGLDIYENEFGAGSYAIVMIEGASVTDSVAVTQELLGLTHVSNVEYLDSVLNQMTFQT
                     LIANFDSQTQTALQTQMDSLVGAGYSYPEAFLEVIDYLRVNLPAPYIALLDPLSLEIE
                     KYYKEGYSKLIVSIDLDQTDLEAETVLDDIVNILENDNLTFYLTGGVVSTIFTRNAIS
                     SEVLKITLIVVPLILLLLLFLTPAYFDLVVFVFAAAVAIILNLGTNALLPNISFITHS
                     IAIVLQLAISLDYIIFIMSRYHEERDKGLSSDEAIKAAMKKVTVPVIASASTTGVSFL
                     ALLFMKFSIGIDIGIVFFKAIVFSLLVALFLVPVLIKIFAKQIDKSRHRVFMPKFGKF
                     AKFVYKFRYLLLAFLMLIVPFAYYFQTHNDFVYGDSALTMSKGSEYYEDTEAIENIFG
                     FDDRIVVLTDKNVVKEALFYQAVSTDESLPLTDIKSGVYYSQMISDPETLAAVLSNFY
                     TDDYEIIILTFDMDPESEEAENVLATLKEHLASSGISEYYLLGSTPVAYETRDIISSD
                     YDFVTLIALIAVMLIIFLTFKNFLMPIILPLVIVASVFVTMSVSYFAGYQVVFLGYLI
                     ISTILLGATIDYAILFGKRYLELRNTYGKHDSIQKAISDTAPSITTSAIIFAVAGLML
                     SVVSSIVTIRQIGGQIAIGAMLALFYVLVLLPQLFYIFDRWIKRSNVERDKR"
BASE COUNT         3712 a         2724 c         2982 g         3443 t
ORIGIN      
        1 tatttgcgaa atagcgaaac tcgtacattc agccggcggt ctcatgtact atgacggggc
       61 caatttgaac gccattcttg gtaaggctcg tcccggagac atgggcttcg atatcatgca
      121 tatcaacctt cacaagacat tttccactcc ccatggcggc ggcggtcccg gttccggtcc
      181 cgtcggcgta agaaagggac tcgagtactt gcttccgaat ccgaaagtcg atatcatgga
      241 cgggcactac tatatcaaag gcgatgccga ttccatcggc aggatttcga tgcatttcgg
      301 taatttcctg gtctttgtga aagcatacgc ctacattctc tctttgggaa acgagaatat
      361 cggaaaagtc ggtgagttgt cggttctaaa cgccaattac gtgatggaat cattgaaaga
      421 cgactacaaa ttgccgatac cgttgcactg caaacatgag tgtgtattcg acggtttgaa
      481 agacgattct accggtatca aaacattgga tgtcgccaaa cgcctgcttg actacggcat
      541 gcatgccccg actatttatt tccccctcct tttccatcaa tcgatcatga tcgaaccgac
      601 ggaaacggaa tccaaacaaa cccttgacga tttcatcatg gcgatgaaat cgattgccag
      661 ggaagccaag gaagatccag aattgctaca tcagcctccg cataccaccc cggtaaggcg
      721 gattgacgaa gtgtacgcgg ttcgtaaaat gattattact tacaaagact actgcgaaaa
      781 taaagaatag caggaggaat aaaacatgta tccgcaatat ttcagcatca tcgatatcgt
      841 catcgtcctc ggagtgatcg ccttcggcgt tattggctgg aaaaaaggtt ttttgttgac
      901 gatcatcaaa atggcatcaa gcgtttttgg acttatcgct tcattgttgc tttcaaaatc
      961 gtttgccaaa gtcctggacg gctggtttgg cgcttcggtc gatacaaaaa tctacgaata
     1021 catgatgtcc cggtccgatc ttttcagcag ttctttgagt gttgacaatg tcaggaccgc
     1081 cctggaaggc atgagcatgc cgacattcct gatcgattgg atcatcagca agatcgattt
     1141 ttcccaaatc ggggtttcga tcgttgatgc catcgaaccg acgattaaat caatcgtcat
     1201 ggtcgttctg gcgttcatca ttttgttttt cggttcgatg cttgttttcc tgatcctgaa
     1261 gatgatcgcc aaactgatca catcgattcc attcatcaaa caggtcgaca agggattcgg
     1321 cgtccttttc ggagtcttca aaatggcggt cgtcacctat gtgcttttgt tcctgttggc
     1381 tctattgatg gcggttcctg ttgttaccaa tttaatcgga gattttgtga caagcgacat
     1441 gcaactgtca accgatgcgt ttcgattatc gaaatggctt tacaacaaca atgtgatcca
     1501 agacttcatc ggtattttct tctaaagtag cggcatcctc aaaggatgcc gtttttttaa
     1561 gcatattttt tgttttcatg gcgctagtga tatataatga aagcgtaaaa aggaaacttt
     1621 ggagatgata ataatgaaat atatagaata tcttaatcaa agacatagcg tgtatgcatt
     1681 atcaaatgaa gaggtcattc cggatgacga tattacgaaa ctagtcggaa gcgtgatcga
     1741 agcgactcct tccgccttca acagccagac ccagagggtg atgatcctgt ttggaccccg
     1801 tcatcttgaa ttctggaaaa tcctcatgaa tgaattgcaa aatatcgtac cgccggaaag
     1861 ttttccgaga acagaggcca agattaacgg cttcgccgcc gccagaggaa cgattctgtt
     1921 tttcgatagt ttggacgaaa ccgagaaatt gatggaacaa ttccctcttt acaagaagaa
     1981 tttcgctttg tggagccagc aacaaaacgg catgttgcag gaaaatgtct gggttgcttt
     2041 ggcaacagaa ggaatcggag ctagtttgca gcattataac gaaatcgtca agggaaaaat
     2101 cgaagcagcc tttggaattc cttccggatg ggatattatc gctcagatgc cgtttggaaa
     2161 aatcatggaa aaaccggaag ccaaaccgaa actgaaactg gaaaccagac tgattgtcag
     2221 agaataaaga aggatcgacc gatccttttt ttatttccgt ctgttgccgg ttttttcttt
     2281 gtgatatact gagtcgggag gtgatcggca tgaaactggt tgaatggctt tcaaacggag
     2341 atccggtaat aaaaaagctg gttgaaagac accttttgga tcatgatgtt ctcaacaagg
     2401 acgaaggata tatccaccgg tatcttgtgt tatttgattg gaagagaaac cgctggtctg
     2461 gtagcgtgta ttctcccaag tggataagca cgcattatac gatgatggaa ctgaaatata
     2521 tggaagtgcc accggataat cccgaataca ctagcggtgt cgaaaccttg atttccggga
     2581 tgtgggaaaa accgggtatg tatggaaagt atcccgatca ggacctgtgt gtcgtcgcaa
     2641 tgatccttgg tatggcttca tacgtcaaat ttcgggatga aaggcttcac gacatggttg
     2701 attacatcct cgcccattgt tttccggacg gaggctggaa ctgtaactgg aatagtgtga
     2761 cacatccggc ttcgaagagt tctcttcaca caacgatatc tgttctcgag gcctttcgcg
     2821 actatttgaa aaacggatac gaataccgcc gggaagaagt tttaacttct attttttctg
     2881 ccgaagattt tgttttacgc aaacatcttt tccgttctga aagaaccgga gaaatcattc
     2941 acaaggcgat gattgaatgc cactatccgg aaagatggca ttatgatctt ttccggggac
     3001 tcgaatactt ccaaagcgtt caaaaaccgt acgaagaaag aatgaacgaa gcgcttgaca
     3061 ttgttgaagc caaattcggg agaaaaggtt atctgaccaa gggaccgttc tatcccgggc
     3121 ttgtgcactt cgatttggaa acatcgagag ccgggagatt cgccacccta aaagctttga
     3181 agattctgaa attctatcgc cgttctgttt ttgatattct aatggatgcc gaaatcggcg
     3241 atccaaaata aaaagcctgt ttatcaggct tttatttctt ttctttgtat aaggcgatag
     3301 cctttttcag ttcgggaatc agatcggatt ggggtatttt cttgacctgc actccgtcaa
     3361 tgaataacag tgcttcatct ttgccgccgc agatggcgac gttggcatcc ttggcttcgc
     3421 ccggcccgtt gaccgcgcag cccatgatgg cgactttgat ttttttatga accgtctgta
     3481 ggtagtcctc gatggcgttg gcgatcggaa acatgtcgta ttggattctt ccgcatgtcg
     3541 ggcagctgat gaggataggc atgtcataaa gggacagatc cgagagtatt tctttgcccg
     3601 ccttgatttc atcgaccgga tcggccgtca gggaaaccct gattgtcgct ccgatacctt
     3661 ttcccagcat gatcccgagt ccgatggcgg agcggatggc tccggaaaaa gcggttcccg
     3721 cttctgtgat gcctaaatgc aaggggtaac ggaaaatctc cgcagccagt tgattggctc
     3781 tgaccgtcgt tgccagatct gatgatttca gggacagaaa aattgccgtg aatccgagtt
     3841 gttccagaag agcgacctgc cttttagccg attcgaccat tgcctcggct ttccggtcct
     3901 tgtaagtttc caacaaatcc ttttcaagtg atccggaatt aatgccaatt cggatcggaa
     3961 cgcctttttt cccgcaggct tccacaactt tcctgatgtt ttcggagctg ccgatgtttc
     4021 cgggattaat tcttattttg tcgatattgt tttcgattgc ttcaagcgcc agtcgataat
     4081 cgaaatggat gtcggcgatc aatgggatcg atatgttctg tttgataacg gcaacggctg
     4141 ccgcatcatc gtgatcgcgg atcgcaacgc ggacaagttc gcaaccgaca ttttcaagac
     4201 gcttgatctg ttcgatcgtc gcgtcaatgt ctttggtttt ggtagtggtc atgctttgga
     4261 cgtaaacgtg attgttgcca ccgatcggaa gatttccgac aaaaatggtt tttgtttccg
     4321 tgcgaaggta catagtctat caccctttat tgattataca ctattttgat attgtttttc
     4381 ctttattcgg ttaattttac tatttttgca tgatttcgtg ataaaataaa agaaagaaag
     4441 cggggggttt gacatgaaaa tcatccgtgg tgtcggcgct tccggacaat gggcggccgg
     4501 aagggtcgtt atactgaaaa aagaaagcaa tatggttgaa agcgaatcga aaacgactca
     4561 ttccgaggaa aaagaacgct tttttaaagc cagacaaaaa gccgaatccg aactcgacag
     4621 attgatcgag aaactcggaa aaagcgatca ggccagtagc cagatttttg cttcgcaccg
     4681 ggaaatgatc aatgatgtga ttttggtcga ccgggtcaag gaaatgatcg accggggcga
     4741 aagcgcggaa atggcggtta cggaagcctg tgatcatttt tacggacttt tgtcacgagc
     4801 agaagacgag tatctgcgcc agcgaaagga cgacgtcctc gatgttaaaa accgattgat
     4861 gcaggctttg tctcaaaaaa aagcggacga agccatcccg gatggttcct atatcgtttt
     4921 ctcagaacgg ttgtttcctt ccgatatcat gaattattcc tcgcgagggg caaacggatt
     4981 cgtttcccgg gtcggttccg tgtattcaca cacatcgatt ttggcgagaa atatgggaat
     5041 tccttatgtc tgccaacttg acgccaccta tgagacgatc aaagaaagcg aatatgtcgt
     5101 cattgatgga aaaagcggag aagtctatct gggactggat gaaaaaacgg ctgcgaatat
     5161 tttggcaagt cgtaacaagg ccatagaaaa aacaatggaa gatgcgttaa gtgttacagc
     5221 ggaaagacaa gaggcgtctt taaaaattaa aattatggcc aatgccaatc gcttggaaga
     5281 tattgatctc atcaaaaaaa ccaaggcttc cggcatcggg ctggtccgca gcgaatatct
     5341 gtttgctgaa aacgagaaat atccaaacga ggaagaacag gaaatcgttt atgaacgctt
     5401 tataaaagct ttggctccgc ttcccgtgac aatcagaaca ttcgattttt catctgacaa
     5461 accgaatcgg ttccgccgct acgaaaaaga ggaaaatccc gctctcggat accgggggat
     5521 aagaatttcc ttagaggagc cggaaaattt caaaatccaa ctccgggctt tgcttagagc
     5581 ttccaaagcc ggaaatcttc ggattttgtt tccgatgatc accggattgt cccaggtgat
     5641 ggctatcaaa aaaattctgc ttgatgccaa gaaggaatta agatcagaaa gcaaagaaat
     5701 tgccgataat atcgaaatcg gtttgatgat tgaaacgccc gccgctgcat tggtggcgga
     5761 tattcttgcc cccgaagtcg actttttcag cttaggaacc aacgatctca tccagtacac
     5821 tctcgcaact gacagacaga atttccgcac ggccggactc tatgacgaaa accatcccgc
     5881 agtgagaaaa ctcatgcaga tgacatgcaa ggcggccgca aaagccagga ttccagtagc
     5941 catttgcggc gagatggcgg agaatttgca tctttcaaat ctgttcttaa cacttggaat
     6001 caatgaacta tccgtgtctt caggcaaggt tgataaaata agaaaggctt atgccgtaac
     6061 agaataaaaa aatccctttt cgatgaaaag gggtttttat tttcctgtgt ttctcacatg
     6121 atcttctgaa gactagcgac ggctccgttg atctcatcgc agatttttgg aagagacgga
     6181 agcgtttttt tcaagagata aggattgatt tcaccgatct ggtaaccgag ttcgttgtaa
     6241 agtgcgatga catttttctt tttatgtccg tccaaccgtt cctcgatttt cgcgtttgtt
     6301 atatgcctga tatcataaat cgtcgatttg acgattcggc agatgacgac atcccttgcc
     6361 cggtcaacaa tcactttggc atgcaaaatg atggcggtat aggtgatgca aagaatcaat
     6421 gtaaggatgc cgaagacaag caaaatgacc gaaaacgaat cgccttcgat aaaaagtatc
     6481 aaataaaaag caatgaaaaa agcatcgatc agaagcgatg cgagtttttg aaacaaggtt
     6541 gtaagcgaaa attttttcat gccgatacca ccttgacatt attataaccg attatatagg
     6601 aaaaagacac gccattttcg aaagtggctc cgaattggga aaagtccgat tttatgatat
     6661 aataaatata caatggtaac attgaaagga ggccatagat gggcaaatcc aaaagacagg
     6721 ttgcgcttga tagagcgcgg gaattgaaaa caagaatcgc cgattacctg gaaatcaggc
     6781 cgaccattcc caaaggtctg gaaaagaaag ccgaaatcgg cgagagacaa agaagaatca
     6841 gggaaacatt gcatgcgact acagaaaatt ggcacgactg gcattggcaa ttaaaaaaca
     6901 gggtgaaaga tgtcgggtcc ttgaaacgtt ttttgaaatt atccgaccgg gagagcgaag
     6961 cgatcgccaa agtatcggaa acttttcgtt gggccatcag cccttactat ctcagtttga
     7021 tcgatcctaa cagcgatcta gacccgataa aattgatggc ggttccatcg gttttggaaa
     7081 ttggtgatcg tgaacacgga ctagacccga tgaaagagga atatacgaat ccagccggat
     7141 gcattacccg ccgttatccg gatcgtctta tcatcaatac gacgaacgaa tgcgccatgt
     7201 attgccgcca ttgccaaaga cggagaaaca tcggagaaaa ggacgccgca agatcgcaaa
     7261 aggaaatcgc ggaatctatc acttacatca aagataatcc ggaaatccgg gatgtgcttc
     7321 tgaccggagg cgatccgttc actttatcca atcaaaggtt ggaatggatt atttcggaac
     7381 taagaaaaat caaacacgtg gaaatcatca gaatcggcac cagaacgccg gtgaccatgc
     7441 cgcaaaggat cacagacaaa ctcgttgcca tgctgaaaaa atatcatccg ctttacataa
     7501 acacgcattt caatcatccg atggaagtaa cggaagaggc taaggaagca tgcgctaaac
     7561 tggccgatgc cggtattccg ctcggaaatc aggcggtttt gttgaacggg ataaacaacg
     7621 atccttatgt catgcgttgt ctcaaccagg aattattgaa aataagggtg aaaccatatt
     7681 acattttcca tgccaaaacg gtaaggggga caacccattt caacacgtct gtcgatgacg
     7741 gcatcgagat catggaatac ctgagaggat acacttcggg actggcgatt ccgtcatata
     7801 tcgtcaatgc ccccggaggg aagggaaaga caccgatttt gccggaatat ttgatatcgc
     7861 gaggtagtga ttatttcgta atccgtacct gggaaggcga gatcgtcagg catgaaaacc
     7921 atccttcaaa agatctcaaa aagatgttgg aagaaaaata tcaaaccttt tgaccgtata
     7981 ggacaacccg gaaaagatat ggtataatac ggctggggaa gtgatttatt ttgtgcggaa
     8041 gattcacttt gggagtgacc gcttccaaac tcataaaata tctcaaagaa aatttcggga
     8101 ttacatgcag tcccgaaact tttgacgtgc ccagattcaa tgtatcccct ggtcaggaca
     8161 tcgtgacgat aatcgccgcc aaggaaggat ttcacagcgg atatctcaat tggagttatc
     8221 gcttctcgac ttccgggcgt ggacttgtca atatccgagc cgaatcgctt gaagagaaaa
     8281 caacgttctt aaaatcattc cagcaaaggc gctgtctcgt tatcgccgac ggtttctatg
     8341 aatggaaaac gacggacggg aaaaagaaag cttactggat ccgtccggcc gatgaaggaa
     8401 tctttctgat ggctggactt tggatttccc agggcgaggg taaggaaaaa cgcttctatg
     8461 cggcaatcgt gacccatcca gccaacagcg acatggaagg aatccaccac cggatgccgg
     8521 tcatcttcac cccggaagat ggtagaaaat ggcttgacag acaagccggg gaagaaaagt
     8581 tacttgatct tataaggatc tatcccgaga aactgacaat cgagcctgtc agcaatcggg
     8641 tgaacgatcc ggacatagac gacgaaagat gcatttggag ggaggactga aaatgtcata
     8701 tataatcatc agggaaacaa aagaaaaata tctgccggat ctcatgaata tgtggaacga
     8761 cggcgaagtc atgaaatacg taaattatcc caacggtctt ggaatgtacg gattcaagat
     8821 ggaaagctgg tataaatggc ttcagagtca gggcgacacc aaacatttca tcatattgga
     8881 cgacgaaacg ggatttttgg gagagacgtt ttattgctac gccaaaaaga acaagcccgc
     8941 ttcgctggat atcaaactga taaagaatgc gagaggcagg catgttggca acctggccct
     9001 gtcatatgcg attgacatgc ttttcaaaaa caccaaggct atgacagcct atgtcgatcc
     9061 caacaaggaa aaccttcctg ccttgtcgct ttacaaacgg ctgggattcg aaaaagtcga
     9121 aaaacaaaac gatctttatc ccgatgccgt atatatggaa atcaaccgcg agaagtggca
     9181 gaaacaaagg cttgaatcca tccgacttgc ccctatagac cgcaacaatg tctttcccgt
     9241 gtgttttctg aaagtcaaag acgaccaaaa gaatttcgtc gccaccaatt ccctgagctt
     9301 ggcgcaggcg gcgtatcaga ccgaatgtat cccccatgca atctattgcg gttataattt
     9361 ggtcggtttt ctgatgcacg caaccgatga gaaagacgga ctccagtgga tataccgtct
     9421 gatgatcgac cgtgattttc aagggctagg atatggggaa aaagccatga aaatcgtgat
     9481 ggataccctg aaaaaacaat cgaagtccaa caggatacgc atcagcttcg agcccgccaa
     9541 caagattgca aggcagctct atgaaaaact cggttttgtc gattccggac aaatggacgg
     9601 cgacgaaaca atttatgaat atacctggca ataatcggag gtggattcca tgaacaattg
     9661 gttgacaatg gatccctata tcgtcttaag tttggccaac atgaaattac gtaattttta
     9721 tcccgatctg gatgctttgt gcgatgatat cggcgtcgat agacgcgctc tcgaggacaa
     9781 acttctgata atcggctatc actatgagag aaaaggaaat cattttgtcg ccgctaaaga
     9841 taaggtgaca atggaagaaa agtgaaaatc aggtacgagg gaaatggcac catagccaaa
     9901 atactaatgg atacggatcc gatccttgtc ccgatttttg ttaaaacgcc attcgtggaa
     9961 tttgaaaccg gatccgatta cttccgtcat ctggccggtg cgatcgtgac ccagcaattg
    10021 tcatcaagag tggcagatgc cattttccgc agattgctcg aatacacaaa aaatgatctg
    10081 tctcccgaga aaatcctagt ctctcccgtt gacgatctcc gttctcaagg cctatcttac
    10141 cgcaaagcgg aatatcttaa attactggct acgagattcg tggaagaccc cggattgttt
    10201 aaaagcttaa gcgagaaaac agacgaagaa gtgacctcga ttttatgtga gataaaggga
    10261 ataggtcaat ggactgccga gatgtttctt atgttcacgc taggcagaga agatgtattt
    10321 tccgcgggag attacggact aaagagggct ttgtccatgt tatacaatag accggtgaat
    10381 gaaaaggaag ccactgccat ggcaaaaaaa tggcagccat accgatctgt ggtctcccat
    10441 tttttatggc attatctaga ctgacatccg caaggatgtt ttttttatct ctattcacgc
    10501 tttcatcaca tataggaaat aaaatgaaat cagataataa ataaacaaat gtttattaaa
    10561 gaaagaaggt gttatcgttg aaatcgctga agaacaagat catcctggcg gtttttttgg
    10621 ttttgacaat agtcgccggg ataatgatca caagaatcaa cgtgaattac gacttgaaac
    10681 aatatttgcc ggatgattct gaaacaattg aaggattgga tatttacgaa aacgaattcg
    10741 gtgctggttc ctatgccatt gtcatgattg aaggcgcatc ggttacggat tccgtcgccg
    10801 tcacgcaaga acttcttgga ttaactcatg tatcaaatgt cgaatatctc gattcagtcc
    10861 tgaatcagat gacttttcag acattgattg ccaatttcga tagtcagacg caaactgctt
    10921 tgcaaacaca gatggacagc ctcgtcgggg ccggttattc gtatccggaa gcatttttgg
    10981 aagtaatcga ttatcttaga gtaaatcttc ccgccccgta tatcgctctt ctcgatccgc
    11041 tttctttgga gatagaaaaa tattacaagg aaggatactc caaactgatc gtttccatcg
    11101 atctcgatca gactgatctg gaagcggaaa ctgtgcttga cgatattgtc aacattttgg
    11161 aaaacgacaa tctgacattt tatctgaccg gcggggtcgt atcgaccatt ttcacaagaa
    11221 atgctatttc aagcgaagta ttgaagatca ctttgattgt cgtacctttg attctgttgt
    11281 tgctgctatt cttgacaccc gcctattttg atctggtggt attcgttttt gcggcagcgg
    11341 tggcgatcat tctcaatcta ggtaccaacg cccttttacc gaatatttca ttcatcaccc
    11401 actcgatagc gattgtgttg caacttgcga tatccctcga ctatatcatt tttatcatga
    11461 gcaggtatca cgaagaaaga gacaagggac tatcctcgga cgaagcgatc aaagcagcga
    11521 tgaaaaaagt caccgttcct gtcatcgcgt ccgcatcgac aaccggtgtc agttttctcg
    11581 cccttttgtt catgaaattc tcgatcggta tcgatatcgg tatcgtcttt ttcaaagcga
    11641 ttgttttcag tttactggtg gctctgttcc tagtgccggt attaataaag atttttgcca
    11701 agcagatcga taaatcccgt catcgggttt tcatgccgaa attcggaaaa ttcgctaaat
    11761 ttgtgtataa atttcggtat ctcttgctgg cgtttctaat gctgatcgtg ccttttgcct
    11821 attatttcca aacccataat gatttcgttt atggggattc cgccctgact atgtcaaaag
    11881 gaagcgaata ttatgaggat acggaagcaa tcgagaatat tttcggattt gatgacagga
    11941 tcgttgtttt gacagataag aatgttgtca aggaggcatt gttctaccaa gccgttagca
    12001 cagatgaaag cctgccttta accgacatca aatccggtgt atattattct cagatgatca
    12061 gcgatccaga aacattagcg gcggttttat ctaattttta taccgatgac tatgagataa
    12121 taattctgac attcgatatg gatccggagt cggaagaagc cgaaaacgtg ttggccaccc
    12181 taaaagaaca cctcgctagt tccggaatat ccgaatatta tcttctcgga tcgactccgg
    12241 tagcatatga aaccagggac atcatatcat ctgactacga ttttgttacg ctgatcgccc
    12301 tgattgcagt catgctgata atattcttaa cgtttaaaaa tttcctgatg ccgatcattt
    12361 tgcccttggt catcgtcgcc agtgtgtttg tgacaatgtc ggtatcgtat tttgctggct
    12421 atcaggtagt gtttttgggg tatctaatca tttctacaat tttattggga gcgacgatcg
    12481 attatgccat attgtttggc aaacgttatt tggagctaag aaacacatat ggaaaacacg
    12541 acagtatcca gaaggcaatc agcgatacag ctccttcgat caccacatcg gccattatat
    12601 tcgctgttgc aggactgatg ctgtcggttg tgtcttcaat cgtgactatc agacagatag
    12661 gtggtcaaat agcgattggc gccatgcttg ccctattcta tgtcctcgtg cttttaccac
    12721 aacttttcta tatattcgat cgttggataa agcgttcgaa tgtggaaaga gacaaacgct
    12781 aaaaaaaata aaaacgggtt taaaaacccg ctgtaatttt cataaaaaag agttggcttt
    12841 tgtcaactct ttgttttttt t
//
LOCUS       DAOPJL010000032        12689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_5220_length_12689_cov_5.243707, whole genome shotgun sequence.
ACCESSION   DAOPJL010000032 DAOPJL010000000
VERSION     DAOPJL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5220_length_12689_cov_5.243707"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..530
                     /gene="greA"
                     /locus_tag="PLP02_04775"
     CDS             60..530
                     /gene="greA"
                     /locus_tag="PLP02_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPF42788.1"
                     /translation="MENTYKLTEQGFQKLKDELAQLKDVDRKQNLEALKEARAQGDLS
                     ENADYDAARDEQARIEARIKEIENILKHSEIINNNGNSKTVMIGKTVKIKFIGNNREG
                     EYTIVGHLEANPLQHKISNESPIGKALIGRRKGQTVNYKAETGAEIAVEIIEIK"
     gene            579..1271
                     /locus_tag="PLP02_04780"
     CDS             579..1271
                     /locus_tag="PLP02_04780"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HPF42789.1"
                     /translation="MKGIIGAMDKEIVYLLDRMENKSQVTMADKEFYHGFISGKEVVV
                     AKSGIGKVNATMTTTMLLTNWQIEAVLNIGVAGGVRNCHTGDIVLADGIVYSDATIAD
                     IDDVPFGKMGNDPLIVTCDEQMLCEARKILEQKKEKHVSGIIASGDRFVTDMAYLADI
                     DQVVDNIVACEMEGMAVAMTCFKFRVPFLSIRGISDMLGEDDQQDKYRISVDEIAKKT
                     SGFALDFLVENV"
     gene            1234..1959
                     /locus_tag="PLP02_04785"
     CDS             1234..1959
                     /locus_tag="PLP02_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HPF42790.1"
                     /translation="MDSHWISWWKMSDLSIMVNSREIQVEIIRKPIKNVYIRAQSKNS
                     LVVSAPVKKSEADILTVINKNIHRIETMLENRKEIPVFSDREAKFMGNLYMADTIPGY
                     IGTVTFDRDRFLFPDLSTKDKEKALHRFYSRHVKQTANDMLASLSPGFREKIGSSPVV
                     FVSRRMKSRLGSCQPKDRIVNLNSILARYEPKYLHAILMHELVHLRVSGHQKDFYKLL
                     LAYEPDYRFLRKQLLNLIKEDGI"
     gene            2071..2628
                     /locus_tag="PLP02_04790"
     CDS             2071..2628
                     /locus_tag="PLP02_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HPF42791.1"
                     /translation="MILLFSNQYFYPDDYQKSIFEIDYQKLWKNGIRYLFLDVDNTLA
                     SYAEMEPGQKIIELFDEIKSLGMSMILLSNNHSERVGRFAEILGVPHIPSSRKPLKAG
                     FKKALKKFPEASKANVWVIGDQFMTDVLGAKRMGLSVTVVDAIDRDKEKWFTKINRMM
                     ERHVLRRIAKANPDLYSQLHLDEKR"
     gene            2634..3752
                     /gene="yqeH"
                     /locus_tag="PLP02_04795"
     CDS             2634..3752
                     /gene="yqeH"
                     /locus_tag="PLP02_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HPF42792.1"
                     /translation="MAELYCKGCGAKIQNDNKTLPGYIPQPLSEIDPSKQVLCQRCFR
                     LRNYSDVITPCLSNEEYLTVINKIKQEDALIVKIVDIFDYTGSFVPAIKQLTGNEDVI
                     LVGNKMDLLPKNVKPERILIWLKQMLALEGFDVLDSILISAKYGHNFDELMELIAKHK
                     GKRNVYIVGCSNVGKSRIVNQILKRYLGYPTDVVTVSSSPGTTIGLIGFPLLDGTMIF
                     DTPGVINKHQYTYYLTRPSYKITVPKKEIKPLVFQLNEGQTLFFGGLARLDIINGETG
                     DKVNVVTYFANTLNIHRTKTARADEIYETKLYSLLTPPFNPEEGVPKWVSHEFRIRDN
                     LKYDIVFSGLGFVTIRAPFYVRTYAPFVVGIYTRPAII"
     gene            3755..4330
                     /gene="yqeK"
                     /locus_tag="PLP02_04800"
     CDS             3755..4330
                     /gene="yqeK"
                     /locus_tag="PLP02_04800"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPF42793.1"
                     /translation="MEYPEIRNLVALKMSADPKRWRHVEGTVRMAEKLALCNHVDCNK
                     AMIASVLHDATKGEPDEMLLMRIHRQFGSFRVENYPRKIWHAVAGVDYAKEDIGINDS
                     DILNAILFHTTGRENMSILEKIVFVSDYLEETRAYADIALRKQAYVDLDKTVEAILKR
                     KKEYVESLHCKAVSLGSDMAFTDSLSEKGGN"
     gene            4330..4665
                     /gene="rsfS"
                     /locus_tag="PLP02_04805"
     CDS             4330..4665
                     /gene="rsfS"
                     /locus_tag="PLP02_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPF42794.1"
                     /translation="MTDRLRLVVKTLEDLKLTDLRIYDFRGFSPYFDFQVVATASNER
                     QVHASINHLREAMPDERIKRVEGADTSRWLLFDLGDIIVHVMHKDEREYYQFEKLFVG
                     REISLSEDL"
     gene            4668..5348
                     /locus_tag="PLP02_04810"
     CDS             4668..5348
                     /locus_tag="PLP02_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF42795.1"
                     /translation="MDFDLLANFYDSFIDPQVYDEYLRLLDKYTSLGTLLDIGCGTGT
                     LSLELAKRGYKVMATDLSEELLDIVDYRAKEENINLEIGVYDMLDPISLKFDSVIASM
                     DVINHLTDLEDVEFGFTNIFKALNNNGVFLFDVLSSEYIDMLDGFKDDDKDMDFHWRS
                     DKGEKEHSIVHTITMTTGDGEKHEIKIYEETHDFLLYEEISKKVGFQILETIRMPERT
                     IFVLQKTE"
     gene            5396..6229
                     /locus_tag="PLP02_04815"
     CDS             5396..6229
                     /locus_tag="PLP02_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPF42796.1"
                     /translation="MFSFVKKYAGLFMLVCGLAGIVMVNIFSAAAKEASPVVTTISTV
                     GILTTSATSTSDLKTKTSFSECYVDIKGEVKEPGVYIVLPGTRIYQVIELAGGLTSYG
                     STASLNLADTVTDGMVVYVPSIQSGTKSEDISLCVQIRGAVENPGIYYVSEGQTVADL
                     VSLAGGLTEEADISSLDFERILSQGYSVIVPSYSQDVADSDTNSGSDETPEGLVNINT
                     ANLDELMTLKGIGLILGQRIIDYRAENGDFACCEDIMLVSGIKDAIYEDIKDFITVGN
                     G"
     gene            6453..8348
                     /locus_tag="PLP02_04820"
     CDS             6453..8348
                     /locus_tag="PLP02_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPF42797.1"
                     /translation="MQEDRFYLCSNGKYLVFCDEANTIFPGDTVEIEGYFFEIESKQI
                     PGGFDYDTYLLSSGIKGVFRTTSLKVTGHHFTLSCIPYDIENYVVSVLPKKAAAYVLL
                     LVFGNDSLTDENLMKKWSELSVVHLFAISGMHVGLIAVFLEKMLRRTGIAKNSRNKLI
                     MIFLLAFNLSTGFRVPIFRASFLYVAAFFQRKEPVVSSLDRLSLLFVGMLLYNPWYME
                     SVGFQLSFLICFCLFLGRKIIGRKKGASLVKMTLMANICALPVTLSLNHGIGLLFLPA
                     NIVFIALVEFLMLPASFITLFFPFVSGAYVYLIEGFETVTYWFSEIDIRIRLGIGGEV
                     SVVMFFLVLFLFCANLYNKRKRMTYLVLFAMTLIFISLPPTIATKVVVFDVGQGDAIL
                     IDSPGCRIVIDTGPEDDYDALIGYLLDQNIRHIDYLVITHNHDDHYGEVADILSFVEV
                     GSVVMGSDPEFSLPCPVTVVEEGDIIGKNSVVLTVISAYTSDSDENNNSLVIYGSVGS
                     DIWLFMADAGTEIESDIMGKYLLDPDIVKIGHHGSDTASSQKFVDSLSPSVCIVSVGK
                     NSYNLPSETVLQRWIEAGALLYNTHIDGTIVFTYPYLSGRRFVFTYEKRRPWFCQMSS
                     FFDLKTG"
     gene            8397..9371
                     /gene="holA"
                     /locus_tag="PLP02_04825"
     CDS             8397..9371
                     /gene="holA"
                     /locus_tag="PLP02_04825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPF42798.1"
                     /translation="MAEGIVYLVYGADRFLVRQETEKIIQKHNIDRSDTEYYDLEETL
                     VDSAVENAMTIPFLSEKKAVILTNANFLSIQSTEKSLKQNQKILVRYLLNPNPTTIMI
                     VQVFSERIDMNKQIVKIIHDKYEIVDCRQEEEKDVFVRIKDEFGKNGFSIAPDALQLF
                     VGRVGSDSEMLKNELDKLMSYAFGQTEITIDMVKEVVYKNPEDHIYQLVNAIIASDRM
                     LMLKVYKELLDSNIDPMWILGAIVSKFQEILYTKELIRSGNKFDEIMKYFSASKGRTY
                     YILKNANTISDEKLQYFLNGLQDLDLKIKTGKIDKEIGLELFIQRLYE"
     gene            complement(9394..9657)
                     /gene="rpsT"
                     /locus_tag="PLP02_04830"
     CDS             complement(9394..9657)
                     /gene="rpsT"
                     /locus_tag="PLP02_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPF42799.1"
                     /translation="MANIKSQMKRNITNEKKRLANASFKSSLKTALKNVEVAVQAGNK
                     ETATEALNTAFMKLDKSVSKGIHHNNYASRQKSRLAKKVQSLQ"
     gene            9955..12132
                     /locus_tag="PLP02_04835"
     CDS             9955..12132
                     /locus_tag="PLP02_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HPF42800.1"
                     /translation="MKKIERKNFRRQDVITDGVRYLVGNGYFGYRGTLDEFTKAEMVS
                     FNLNGLYDRYMELWRESVNAYNPLYTFMIVKNRELNPTKLPPESHIQGIDIETGLFFR
                     ETVFAIGDAKIKIRSERFADQKNKELLYARYVFSSSSDIAADIYTGIDLDIYNLSGMH
                     LEIQDKVDEAGFFFVKAKTMELGIPIVVGETSSTSFSAEPETIIQNQKLLRRFRLKLL
                     AGEKYEITKYTGVCHTRFDSYEHLDMLVTKAQKLGFRHKFDENRQFWLDKWKLAGIEI
                     SGDEEAEIAVNYSIYQLISSRPYSDYVSIPARGVSGQAKKGAVNWDAEIFMLPFFMNT
                     DPESARHMIMYRILGLQGAIDKATQYGYKGAFYAMESQERGSEACSDYNVTDAITGEP
                     IRTYFRERQIHLNGAMVYALANYIRRTDDVSVLFLGGLEMIIECARFYLDYLEYNKDT
                     KLYEALNVVGPDEYHEKIDNNAYTNYMIDYVFEQTDALLDYARQSSPKKVATLIKEKD
                     YTQDIVKMRIVREKLFLPEPDEKNIVEQFAGYFDLEDVPLTEIRKQMRHPNEYLGGED
                     GLATPTKIIKQSDVVTMMVLLPERFSRVVKKANFAYYEPLTESDHSLSASMQALIACD
                     VGRPNFAYPFFLSSAQIDMLGKGKEFSGGIYIGGTHLAACGGAYLSLIYGFCGLKHRD
                     FILMADTRLTSKIKEVRFHVYVRGRIASVKVSSGVAQVTWEEE"
     gene            12132..>12689
                     /gene="pgmB"
                     /locus_tag="PLP02_04840"
     CDS             12132..>12689
                     /gene="pgmB"
                     /locus_tag="PLP02_04840"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HPF42801.1"
                     /translation="MEYKAFIFDLDGVIVSTDEYHFIAWKEIASREGIPFDRETNNSL
                     RGISRMASLDIILNKADKTYSEEKKQELALNKNAIYQLLLQNLGPEDLLPGVMEVLKH
                     LRLAGYLLAVGSSSRNTKMILARLGLNDYFEAVADGTEIRHSKPDPEVFLKAAEKLGI
                     EPEKCVVIEDATAGIEAAKRGGMLAI"
BASE COUNT         3629 a         2731 c         2973 g         3356 t
ORIGIN      
        1 ttaagcaaat gaattatgct ataataaaaa tcacttaata tttaggagag cggatcacaa
       61 tggaaaacac ttataaactg acggaacaag gctttcaaaa actgaaagac gaattggccc
      121 aattgaagga cgtcgaccga aaacaaaacc ttgaagccct gaaagaagcc agagctcagg
      181 gagacttgtc cgaaaacgcc gattatgatg ccgccagaga cgagcaagcc cggatcgagg
      241 cgaggatcaa ggaaatcgaa aacatcctca aacactcgga aatcatcaac aacaacggca
      301 actcgaagac ggtaatgatc ggaaaaacag tcaaaatcaa atttatcggt aataaccgcg
      361 aaggcgaata taccatagtc ggacatctgg aggccaatcc ccttcagcac aaaatttcga
      421 acgaatcacc gatcggtaaa gccctgatcg gtcgccgcaa aggccagacc gtcaactaca
      481 aggcggagac cggcgccgag atcgccgtag agatcatcga aataaaataa cgggaaaatg
      541 agaaaatatt tctcgttttt cttttaaagg aggcagccat gaaaggaatc atcggagcca
      601 tggacaaaga aatcgtctat cttttggatc gaatggaaaa caagagccaa gtgaccatgg
      661 cagacaagga attttatcac ggattcattt ctggcaaaga agtcgttgtg gccaaatccg
      721 gaatcggaaa ggtcaacgcg actatgacga cgaccatgct tctgaccaat tggcaaatag
      781 aagcggtgct caatatcggc gtcgccggag gagttaggaa ctgccatacc ggcgatatag
      841 tgctggcgga cggaatagtg tattccgatg ccacgatcgc agacatcgac gatgttcctt
      901 tcggaaaaat gggcaatgat cccctaatcg ttacttgtga cgaacaaatg ctttgtgaag
      961 ccagaaaaat tctcgaacaa aaaaaagaaa aacatgtttc cggcatcatc gcgtccggag
     1021 atcgcttcgt caccgacatg gcatatctgg cggatatcga tcaggtcgtc gataatatcg
     1081 tcgcctgcga aatggaaggc atggccgtgg caatgacctg tttcaagttc agggtgccct
     1141 ttttgtcgat tcgcggtatc agcgatatgt tgggcgaaga cgaccaacag gataaatacc
     1201 gtatatcggt cgatgaaata gcaaaaaaga ccagtggatt cgcactggat ttcctggtgg
     1261 aaaatgtctg atctctcgat catggtcaat tcacgggaaa tccaggtgga gattatccgc
     1321 aaaccgatca aaaacgtgta tatcagggcg caatccaaaa acagtttggt agtttcggct
     1381 cccgtgaaaa aaagcgaagc ggatatccta actgttatta ataagaatat ccatcggatc
     1441 gaaacgatgc tcgaaaaccg gaaagagatt ccggtttttt ccgatcgaga agcgaaattc
     1501 atgggaaacc tatacatggc tgacacgatt cccggataca ttggcacggt gacattcgac
     1561 cgagaccgtt ttctttttcc cgacctgtct acaaaagaca aggaaaaagc ccttcacaga
     1621 ttttactccc ggcacgtgaa acaaaccgcc aatgacatgt tggcttcgct ttcgcccgga
     1681 ttcagggaaa aaatcgggtc ttcaccggta gtgtttgtca gccggaggat gaaaagccgg
     1741 ttgggtagct gtcagccgaa ggataggata gtgaatctta acagtattct ggctcgctac
     1801 gaaccaaaat atcttcacgc catccttatg catgaactgg tccatctcag agtatccgga
     1861 caccaaaagg atttttacaa gttgttgctg gcttacgagc cggattaccg tttcctgaga
     1921 aagcaacttt tgaacctgat aaaggaagac gggatttaaa aggcgtactt ttgtaaaacc
     1981 cgttccaata taatgcatcc ggacgagaaa aaatggactt ttccaagaaa atccggtata
     2041 atataattaa aattcggagg tagaagtcta atgattttgt tattttccaa ccagtacttt
     2101 tatccggatg actatcaaaa aagcattttt gaaatcgatt atcaaaaatt atggaaaaac
     2161 ggcattcgtt atcttttcct ggacgtggac aatactttgg catcttacgc cgaaatggag
     2221 ccgggacaaa agataatcga actgtttgat gagataaaat cgcttggcat gtcgatgata
     2281 ttgctatcaa acaaccattc agaaagagtc gggcgtttcg cggaaattct cggagttcct
     2341 catattccga gttccagaaa gcctctcaaa gccggtttca aaaaagcgct gaagaagttt
     2401 ccggaggcat ccaaagctaa tgtgtgggtc atcggcgatc aatttatgac cgatgttctc
     2461 ggcgccaagc gtatgggcct ttcggtcacg gtcgtcgatg cgatcgaccg cgacaaggaa
     2521 aaatggttca ccaaaataaa caggatgatg gagagacatg tattgagacg gattgccaag
     2581 gcgaatccgg atttgtactc gcaattgcac ctggacgaaa agaggtagta gttatggcag
     2641 aactgtattg caaaggttgt ggagccaaga tccagaacga caacaagact ttgcccggat
     2701 acattcccca gccgttatcg gaaattgatc cctctaagca ggttttgtgc cagaggtgtt
     2761 tccggttacg caattacagc gacgtcatca ctccctgtct gtcgaacgaa gaatacttaa
     2821 ccgtcatcaa caagataaaa caggaagacg ccctgatcgt caaaattgtc gatatattcg
     2881 attataccgg gtctttcgtt ccggcgatca agcagcttac cggtaacgaa gatgtcattt
     2941 tagtcggcaa caagatggat cttttgccga aaaacgtcaa gccggaacgc attcttatct
     3001 ggctgaaaca gatgctggca ctggagggct tcgatgttct cgacagcatt ctcatttctg
     3061 ccaagtacgg acacaatttc gatgaattga tggaactcat tgccaagcat aagggcaaac
     3121 gcaatgttta tatcgtcggt tgttcgaatg ttggcaagtc caggatcgta aaccagattc
     3181 tgaaacgcta tctcggttat ccgaccgatg tcgtcaccgt cagttcctcg ccgggaacga
     3241 cgattggatt aattggtttc cccctgctgg acggaacgat gatatttgac actcccggtg
     3301 taattaacaa gcaccagtac acatattatc ttaccagacc gtcatacaag ataaccgttc
     3361 ccaagaagga gatcaagccg cttgtattcc aacttaacga aggacaaacc ctgtttttcg
     3421 gaggtctggc aaggcttgac atcatcaacg gggaaaccgg cgacaaagtc aatgtcgtca
     3481 cgtatttcgc caataccctg aatatacaca gaacaaaaac cgctcgggcg gatgaaatct
     3541 atgaaaccaa gctgtattct ttgctgacgc cgcctttcaa tcctgaagaa ggggttccca
     3601 aatgggtatc ccatgaattc cggattcgtg acaacctcaa atacgacatt gtgttctccg
     3661 gcctcggatt cgtaacgatc agagctccgt tctatgtccg gacttacgct ccgttcgtag
     3721 tcgggatcta tacaagaccg gctattatct gacaatggaa tatccggaaa taagaaactt
     3781 ggtcgctttg aaaatgtctg ccgatccgaa acgttggcgc cacgtagaag gaactgtcag
     3841 gatggcggaa aaattggcct tatgcaacca tgtcgattgc aacaaggcaa tgatcgcttc
     3901 agtgcttcat gatgcgacca aaggcgaacc ggatgaaatg ttgcttatgc gcatccaccg
     3961 acagtttgga tctttccggg ttgaaaatta tccccgcaag atctggcatg ccgttgcggg
     4021 agtcgactat gccaaagaag acatcggcat caacgattca gacatcctga atgccattct
     4081 cttccatacg accggaagag aaaatatgtc gatattggaa aagattgtgt tcgtaagcga
     4141 ctatctcgaa gagaccagag cctatgccga catcgctttg agaaaacagg cttatgtcga
     4201 tcttgacaaa acagtagaag ccattttgaa aagaaaaaag gaatatgttg aaagtttgca
     4261 ctgcaaagct gtgtcgctcg gtagcgacat ggcttttacc gactcattat ccgaaaaggg
     4321 aggaaactga tgacagaccg tcttcgcttg gttgttaaaa cattggaaga tctcaagctg
     4381 accgatctac gcatatatga ttttcgtggg ttttcccctt attttgattt tcaagtcgtc
     4441 gccacggctt caaacgaaag acaggtgcac gcatcgatca atcatcttcg cgaggcaatg
     4501 cccgatgagc gtataaaaag agtcgaaggc gcggatactt ccagatggct tttgtttgat
     4561 cttggcgata tcatcgtgca tgttatgcat aaggacgaaa gggaatatta ccaattcgag
     4621 aaactgttcg tgggccgcga aatttctctt tccgaggatc tctaaacatg gatttcgacc
     4681 ttttagccaa tttttacgat tcattcatcg atccgcaggt ttatgacgaa tatcttcgtc
     4741 ttttagacaa atacacttcg ctgggaacat tgttggacat cggctgtggc accggcacgc
     4801 tttctctgga actggcaaaa cggggataca aagtcatggc aaccgatctg tccgaggaat
     4861 tgctcgacat tgtcgactat cgggccaaag aagaaaatat caatctggag atcggcgtat
     4921 acgacatgct tgatcccatt tccctgaaat ttgacagcgt catcgcttca atggatgtga
     4981 tcaatcatct gaccgatctt gaagacgtgg aattcggttt taccaacatc ttcaaggctc
     5041 ttaacaataa cggtgtcttt ctcttcgatg tcctgtcatc cgagtatata gacatgctcg
     5101 atggattcaa ggatgatgac aaagatatgg atttccattg gcgctcggac aaaggcgaga
     5161 aagagcacag tatcgttcat acaataacaa tgacgaccgg agacggtgaa aagcacgaaa
     5221 taaagatcta tgaggaaacc catgatttcc tgctatacga ggaaatctcg aaaaaagtgg
     5281 gatttcagat tttggaaacc atccggatgc cggaaagaac gatattcgta ttgcagaaaa
     5341 ccgaataaag aaaaggaata aaggccggcg tatcattttg acgaggtgag gattgatgtt
     5401 ctcgtttgtc aaaaagtacg ccggcctttt catgctcgtt tgcggtttgg ccggaatcgt
     5461 catggtgaat attttttcgg ccgctgccaa agaagcttcg cctgttgtca caaccatttc
     5521 tacggttggt attctcacga cgtccgccac atcgacttcg gatttaaaga caaaaacgtc
     5581 tttttccgaa tgttatgtcg atattaaggg ggaagtgaag gaaccaggtg tctatattgt
     5641 ccttccgggc accaggattt accaggtcat cgaactggcc ggcggattga cgtcttatgg
     5701 atccaccgca tcgctgaatt tggccgatac ggtaaccgac ggcatggttg tttatgttcc
     5761 gtctatccaa agcggcacca aaagcgagga catatcgctt tgcgtacaga tcaggggcgc
     5821 agtggaaaat ccgggcattt actatgtgtc cgaaggccaa acggtagccg atctggtttc
     5881 tttggccgga ggcctgacag aagaagcgga tatttccagt ctggatttcg aaagaattct
     5941 gtcacaggga tattcggtca ttgttccgtc atattcgcaa gatgtagcgg attcggatac
     6001 aaacagcggt agcgacgaaa cgccggaagg tcttgtgaac atcaatactg ccaaccttga
     6061 cgaattgatg accctaaaag gaatcggttt gattttggga cagaggatca tcgattaccg
     6121 agcggaaaac ggtgatttcg cctgttgcga ggatatcatg ctagtttccg gaatcaagga
     6181 tgcgatctat gaagacatca aagatttcat tacggtcgga aacgggtaag tttgtccatc
     6241 tggcactttc tctttcctta ctgtatttcg gggtttttca cttctttatt tttctctttt
     6301 tgattttaga aatagtattt ttatggaaaa gggcccgcgg gctttttttc ctttttctga
     6361 ttctcggttc ctttttttgt ctacgtcttt tctcactttc gcaaaacctt gttatatccg
     6421 gggaagcttt tcttgccgga acggtttcag aagtgcaaga ggaccggttt tacctgtgtt
     6481 caaacggaaa atatctggtt ttttgtgatg aagcaaatac aattttcccg ggcgacacag
     6541 tagaaatcga agggtacttt ttcgaaattg aatccaaaca gattcccggc ggattcgatt
     6601 atgacacata tttgttgtca tccggaatca aaggagtttt caggacgaca tcgcttaagg
     6661 tgaccggaca tcatttcact ctttcctgca ttccttatga catcgaaaat tacgtcgttt
     6721 ccgttttgcc aaagaaggca gcggcttatg tgctgttgct ggtgttcggg aacgattccc
     6781 tgactgacga gaatcttatg aaaaaatgga gtgaactctc ggttgtccat ctgtttgcga
     6841 tatcaggaat gcatgtcgga ttgattgccg tgtttctaga gaaaatgtta agaaggacgg
     6901 ggatcgccaa aaatagtaga aacaaactta taatgatctt tcttttggct ttcaatttgt
     6961 cgactggatt cagagttccc atatttcggg cgtcttttct ttacgtcgcg gcctttttcc
     7021 aaagaaaaga acccgtcgta tcgtctcttg accgattgtc gttacttttt gttggaatgc
     7081 ttttatataa tccctggtat atggaaagcg tcggtttcca actaagcttt ctgatctgct
     7141 tttgtctttt tctcggaaga aaaatcatcg gaaggaaaaa aggcgcaagt ttggtgaaaa
     7201 tgactctgat ggccaatatt tgcgccttgc ccgtcacgct ttctttgaat cacgggatcg
     7261 gtttgctatt tttaccagcc aacatcgtct tcattgcttt ggtggaattc ctgatgttgc
     7321 cggcgtcttt catcactctg ttttttccgt ttgtatcggg cgcttatgtc tatttgattg
     7381 aaggctttga aaccgtgact tattggtttt ccgaaatcga tatccgaatt aggttgggaa
     7441 taggcggcga agtgagtgtc gtcatgtttt tcctggttct ttttcttttt tgtgctaatt
     7501 tatacaacaa aagaaaaagg atgacatatt tagtgttgtt tgcgatgacg ctgattttca
     7561 tttcccttcc gccaaccatt gcgacaaaag tcgtcgtatt cgatgtcgga caaggtgatg
     7621 caattctgat tgattccccg ggctgtcgca tcgtgattga taccggacca gaagacgatt
     7681 atgacgcact gatcggatat cttctcgatc agaacatccg tcatattgat tatctggtca
     7741 tcacgcacaa ccatgatgat cactatggcg aagtagcgga tattttatct tttgttgaag
     7801 taggctccgt cgtgatggga agcgatccgg agttttcttt gccatgcccg gttacagttg
     7861 tcgaagaagg agatataatc ggtaagaatt ccgttgttct tacggttatt tccgcctata
     7921 cttccgatag cgacgaaaat aacaattccc tggtgattta cggatcggtt ggttcggata
     7981 tatggctttt catggctgat gccggaacag aaattgaaag tgatattatg ggaaaatacc
     8041 tacttgatcc cgatattgtc aagattggcc accacggtag cgatacggct tcctctcaga
     8101 aattcgtgga tagtttgtcg ccttccgtgt gtatagtgtc ggtagggaaa aacagttaca
     8161 atcttcccag tgaaacagtg ctgcaaagat ggattgaagc cggagctctc ttgtataata
     8221 cccatatcga cggaacaatc gttttcacat atccttatct ttccggtcgt cggtttgttt
     8281 tcacatatga aaaaagacgc ccatggtttt gtcaaatgtc gtcttttttt gatttaaaga
     8341 ccggataaac ttttttccgg acacctttta tgataaaata aacattggtg atcatcatgg
     8401 ctgaaggcat tgtttacctg gtatacgggg ctgatcgatt tctcgttcgc caggaaaccg
     8461 aaaaaatcat tcaaaaacac aacatcgacc gttccgacac cgaatattac gatcttgaag
     8521 agactttggt cgatagcgct gtcgaaaacg cgatgacgat tccttttcta tccgaaaaga
     8581 aagccgtcat tttgacgaac gccaattttc tttctataca gtcgacggaa aagagtctca
     8641 agcaaaatca aaaaatcctg gtcagatatc tattgaatcc caatcctacg accatcatga
     8701 ttgttcaggt gttttccgag cgtatcgaca tgaacaaaca aatcgtcaaa atcatccatg
     8761 acaaatacga gattgtggat tgtcgccaag aagaagaaaa ggatgttttc gtgagaatca
     8821 aagatgaatt tgggaaaaac ggattttcga tcgcaccaga cgccctgcag ttgttcgtgg
     8881 gccgggtcgg aagcgattcg gaaatgctta aaaacgaact tgataaactg atgtcctatg
     8941 ctttcggaca aacagaaatc accatagaca tggtgaaaga agtcgtatac aagaatccgg
     9001 aagaccatat ctaccagctg gtgaacgcca tcatcgcctc cgacaggatg ctgatgctca
     9061 aagtatacaa ggaactattg gattccaaca tcgatccgat gtggattttg ggagcgatcg
     9121 tttccaaatt tcaagagatt ctttatacca aggaattgat ccggtccgga aacaaattcg
     9181 atgagataat gaaatatttt tccgccagca aaggaagaac atactatatc ctgaaaaatg
     9241 ccaatacgat ctccgatgag aaactacaat attttctgaa tgggctgcaa gaccttgatt
     9301 tgaagattaa aaccggaaag atcgataagg agatcggcct ggaactcttc attcaaagac
     9361 tgtacgaata aaaaaaagcc cgtcgggctt ttattactgg agactttgaa ctttttttgc
     9421 caggcgcgat ttttgcctgg aggcgtaatt gttgtgatga atccctttgg aaaccgattt
     9481 gtccagtttc atgaacgcag tattcagcgc ttcggtggca gtctctttgt taccagcctg
     9541 cacagccact tcgacgttct tgagagcggt cttcaaagaa gatttgaacg aggcattggc
     9601 gaggcgtttc ttctcgtttg tgatattgcg tttcatctgt gatttgatat tagccataat
     9661 ttcacctcca ttgacatcag cggtattatt ttaccaaata aacatcaaaa aagcaactat
     9721 catatttcaa ttattttcgg cattgacacc aaaacggata aatcctgaat ttgctccaat
     9781 ttacgcgttt tgacgggctg tggcgggaaa tatgatgctg gaagttgccg cgcccagtcg
     9841 acaaatgctg gttgcctctt gaaaatgctg gccttgtgcc ggtataatta taaatatgct
     9901 gtgccttcac aatgaatcaa ccgacgtcta tgatcggccg aaggaggatt gcggatgaaa
     9961 aaaatcgaaa ggaaaaactt tcgtagacag gatgtcatca ccgatggtgt aaggtatctt
    10021 gtcggtaacg gctattttgg ataccgcgga acccttgacg agtttaccaa ggcggagatg
    10081 gtttctttca acttgaacgg cttatacgac cgttatatgg aattatggcg cgaatccgtc
    10141 aatgcctaca atcccttgta cactttcatg attgtcaaaa atcgcgaact gaatcccaca
    10201 aaattgccgc cggaatcgca tattcaaggc attgacatcg agacgggcct gtttttccgc
    10261 gaaactgtgt ttgccattgg agatgccaaa ataaaaataa gatcggaacg gtttgccgac
    10321 cagaaaaaca aggaactgct atatgcgcgt tatgttttca gcagttcttc ggatatagct
    10381 gccgatattt ataccggaat cgatttggat atctacaacc tatccggaat gcatctggaa
    10441 attcaggaca aggtggatga ggccggattc tttttcgtca aggcaaaaac gatggaactt
    10501 ggaattccga tcgttgtcgg cgaaacatcg tcaaccagct tttccgctga accggagacg
    10561 atcatccaaa atcagaaact gttgcgtcgt ttccgattga aactcttggc cggagaaaaa
    10621 tacgagatta ccaaatacac cggcgtctgt catacgcgtt tcgacagcta cgaacacctt
    10681 gacatgttgg tgacaaaagc tcagaaactc ggcttccggc ataaatttga cgaaaaccgg
    10741 caattttggt tggataaatg gaaattggcg ggaatcgaaa tctccggtga tgaagaagcc
    10801 gagatcgcag tcaattattc gatttaccaa cttatttctt cccggccgta ttctgactat
    10861 gtttcgattc cggcccgcgg agtttccggt caggccaaaa aaggcgccgt aaactgggat
    10921 gcggagatat tcatgttgcc attcttcatg aataccgatc cggaatcggc aagacatatg
    10981 atcatgtacc ggattctcgg actccagggg gccattgaca aggctactca gtacggatat
    11041 aaaggtgcat tttatgcgat ggaaagccaa gaacggggaa gcgaggcatg cagcgactac
    11101 aacgttaccg atgccataac cggcgaacca atcaggacat atttccggga acgccagatc
    11161 catcttaacg gagccatggt ctatgcgctt gccaactata tcaggcgcac cgacgatgtt
    11221 tccgtattgt tccttggcgg tcttgaaatg atcattgaat gcgcgcgttt ttatctggat
    11281 tatttggaat acaacaaaga caccaaacta tacgaagccc tgaacgtcgt gggcccggac
    11341 gaatatcacg aaaagatcga taacaacgcc tacaccaatt acatgattga ttacgtattc
    11401 gaacagacag atgctttgct ggattatgcc agacaatcgt ctccgaagaa agtcgccacc
    11461 ctgatcaagg aaaaagatta cacccaggac atcgtcaaaa tgaggattgt cagagaaaag
    11521 ctctttttgc cggaacccga cgaaaagaat atcgtggagc aatttgccgg atatttcgat
    11581 ttggaagatg ttcccttaac ggaaattagg aaacagatgc gtcatccgaa tgaatatttg
    11641 ggcggagaag acggactggc gaccccgacc aaaatcatca aacagtccga tgtcgtaacc
    11701 atgatggttt tgttaccgga aagattttcc cgggtcgtca aaaaagccaa tttcgcctat
    11761 tatgaaccgc tgacggaatc ggatcacagt ttgtcggctt ccatgcaggc gcttattgcc
    11821 tgcgatgttg gaaggccgaa ctttgcttat cctttcttcc tttcgagtgc tcagatcgat
    11881 atgcttggca aaggcaagga attttccgga ggcatttata tcggaggcac acatctggcc
    11941 gcctgcggcg gagcatattt gtctctgatt tacggatttt gcggactcaa gcatcgtgat
    12001 ttcattttaa tggccgatac aagacttacc agcaagatca aggaagtccg tttccatgtc
    12061 tatgtgcgcg gccgtattgc cagcgtaaaa gtttcaagcg gcgtggcgca agtgacctgg
    12121 gaggaagaat aatggagtac aaggctttca tattcgacct ggacggagtc atcgtatcaa
    12181 ctgacgaata ccatttcatt gcctggaagg agattgcctc aagggaggga attcctttcg
    12241 atagggaaac caataacagt ttgcgtggca tttccagaat ggcttcttta gatatcattc
    12301 ttaacaaggc tgataaaact tattcggagg aaaaaaagca ggagcttgcc ctaaacaaaa
    12361 atgcgatcta ccaactgctt ttgcagaatc tcggtcccga agatctgctt cctggtgtca
    12421 tggaagtgct caagcactta cgcctggccg gttatctttt agcggtcgga tccagcagca
    12481 ggaacacgaa aatgatactg gccagactcg gactgaacga ttacttcgaa gccgttgcgg
    12541 acggtacgga gataagacat agcaaaccag atccggaagt gtttctgaag gcggcggaaa
    12601 aattggggat cgaacccgaa aaatgcgtcg tcatcgaaga tgccaccgcc gggatcgaag
    12661 ccgccaaaag gggaggcatg ctggcaatc
//
LOCUS       DAOPJL010000033        12473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_5330_length_12473_cov_10.933403, whole genome shotgun
            sequence.
ACCESSION   DAOPJL010000033 DAOPJL010000000
VERSION     DAOPJL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12473
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5330_length_12473_cov_10.933403"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLP02_04845"
     CDS             complement(<1..787)
                     /locus_tag="PLP02_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HPF42802.1"
                     /translation="MMDEYKLHKHIICIDLKSFFASVECSLLGLDPFKTPLVVTDKAR
                     GSGAIVLAVSPYLKTMGVPGRCRIRELPKNVDIIYRKPRMQTYLSFSAQIIGIYLRYI
                     SDDDIYVYSIDEAFMDFTNYLEYYHKNDLEIAKELMDTIYKETGIYSSCGIGPNMLMA
                     KLALDIGSKNKKDFVAKWDYEDIPEQLWPVSPLSKMWGIGRNMEARLNRMGFYKIGDI
                     AGSDADKLKKRFGVLGEELFYHTHGIDMSLIQDKTKIKPFSKSY"
     gene            complement(923..2620)
                     /locus_tag="PLP02_04850"
     CDS             complement(923..2620)
                     /locus_tag="PLP02_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF42803.1"
                     /translation="MKRKITSQSILVVSVALLAFVVGSIFLVRRNLNNVTEFNLRYYL
                     EIVKTDYDNDLTPAEIIEKYDNIEDYLRITFMDDTGIVLADSSATELENHLTRPEFQD
                     PGTIYIRKSVTLDQQMMYLAFQFDDGDYVRVSVPINSILPFFNDFIGLLLLIAAIIAV
                     LAYFLSKSLIDRTMAPFNDLKKVLQAVGEGKYNELLPVSKYDEINGLLVEINEINKTI
                     SDNIVSLNAEKQKSDFLLDHMNQGLCMLDYQENIVLVNSYLAHLFNYSSENHLYRNYN
                     FLFRSQPLQQAVDRVYESKGSTTLVISVNDSYYSVGVAYEEKDWRNQPGVVMIFTDVT
                     IIKNMETLKRDFFVNASHELKSPLTSIMGSSELITSGIIHNQDEIADLASRILDESKR
                     MNKLVLDMLDLSKYENYIPLKAPSTIDLQAVICEVVDKLEPQAQAKNIKLINQSVPMT
                     FSANQEHMEQLIRNLMENSIQYGKESGWVKIETNATDKEITIKVTDNGIGIPSTDQSR
                     VFERFYRVDKARSKKSGGTGLGLSIVKHIVLIYQGRIELESKEGKGTEITIVLPEQI"
     gene            complement(2617..3303)
                     /locus_tag="PLP02_04855"
     CDS             complement(2617..3303)
                     /locus_tag="PLP02_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF42804.1"
                     /translation="MGSLIYTVEDDINIQYVIKIALQNSGFEVEVFGDAESFYTQMEQ
                     KLPDLVSLDIMLPGEDGIGILQKMKNTVMWKNIPVLIVSAKSSEIDKVVGLDLGADDY
                     MIKPFGVLELVSRIKALLRRSKKGGTEKEIEVSGLILFDSERTCTFKGKSVVMTAKEY
                     ELLKLLVSNNGQTVTRNEIVETVWGYDFIGESRTVDAHIKTIRQKLASIGLAEAPILT
                     VRGIGYKAIL"
     gene            complement(3319..3972)
                     /gene="phoU"
                     /locus_tag="PLP02_04860"
     CDS             complement(3319..3972)
                     /gene="phoU"
                     /locus_tag="PLP02_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPF42805.1"
                     /translation="MQRTKFDSELEHLKLDLVKMGDLVIENIHESFRSFLDMNTELAT
                     MIIEKDKEVNACEAMIEKECLRIILREQPVAKDLRLITSVLKMITDLERIGDHAADIA
                     KITVFMETEKQNTKTEDLTEITGKTQDMIRDALESFTSLDIQLAKAVIKSDDEVDALF
                     DKVRNDVVKAIRMQTMDADYAIYLMMVAKYLERIGDHAVNIGEWVIFAITGAHNYDI"
     gene            complement(4031..4786)
                     /gene="pstB"
                     /locus_tag="PLP02_04865"
     CDS             complement(4031..4786)
                     /gene="pstB"
                     /locus_tag="PLP02_04865"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPF42806.1"
                     /translation="MEQVFEIDELNLRYKDKQALINIKMDIEAKKITALIGPSGCGKS
                     TLLRCLNRMNDLIDNCFIEGYVRYENNDIYTKNYDVIELRTKVGMVFQKPNPFPMSIF
                     DNVAYGPRCQGIKGKKELQDIVKKALVNAALYDEVKDRLNDNALSLSGGQQQRLCIAR
                     TIAMEPEVILMDEPTSALDPIATAKIEELIVGLKDKYTIVIVTHNMQQAARVSDYTAF
                     FLLGRLVEFGTTDQIFGQPKQKETEDYITGRFG"
     gene            complement(4795..6009)
                     /gene="pstA"
                     /locus_tag="PLP02_04870"
     CDS             complement(4795..6009)
                     /gene="pstA"
                     /locus_tag="PLP02_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPF42807.1"
                     /translation="MMKKRRKNILDRGAYAGIATISLFGLLILMLLFSYIIDKGANLL
                     SWDLITGDYYAATYDSYYEGELNQDGYSANREFESDVFYSEKWGIALKDDINFEGKDY
                     VLVVYVSDESPLANLRDKNDEKNYVAISGGQSMVKIIFSNNTVMYSKNGAKAAIELFD
                     NADGIKDMITTTAGSGIRGSLVTTGYLIFFTMIIAMPLGVFTAIYLHEFAPKDKWYIN
                     VLRRSVELLTGVPSIIFGLLGAAVFIPLTSTLTKASGGNIISGSLTLAVIVLPVIISS
                     VEESLKVIPDDYRQASFALGANKTQTTFKIILRCSLPGILTATLLSVGRIIGESAALI
                     YAIGTVIKDNIVVTEKSTSLAVFIWSIMAGEVPNFELACAVSLIILIVVLILNMTVKL
                     ISYKLTFDRNRG"
     gene            complement(6006..6929)
                     /gene="pstC"
                     /locus_tag="PLP02_04875"
     CDS             complement(6006..6929)
                     /gene="pstC"
                     /locus_tag="PLP02_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPF42808.1"
                     /translation="MVCRTIDKKKNSRDKLTHILLLVPSVISASFVFFIIIFVAKRGV
                     APFLVSSYGSESVNLLRFLTGTTWYVPPTAYGIFFIVVNTIYVVALAAIIAVVISVTT
                     ALFIAKMTNRIIGGFLKTVIELLAAIPSIIYGVFGLGVITKFVIGLADVFGSQTAGGI
                     STLSTILVLAIMIYPTVTLMSITAINAVDTNLEKGSLALGASRIQTYFKVTLLTAKSG
                     IFSGVILGIGRALGEATAVSMVAGNAGSGPSFDLFSTTRTLTSTMLLGLKETSGVDYD
                     IRFSVGIVLVFVILFGNLLLNHFKKRIGKYS"
     gene            complement(7004..7954)
                     /locus_tag="PLP02_04880"
     CDS             complement(7004..7954)
                     /locus_tag="PLP02_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF42809.1"
                     /translation="MKKILFGIMIFMVAAMTACAGNTSDVTTTEAFDSSRNITVYTRD
                     TTSGTRDAFFSGIDFDDAVADNSVLATSYVEVDSNGSMITAVTNDIYGIGYISLASLL
                     ESGLTGLAFDGIEATEENVINETYALKRPFNYIVRSDWTGMDREQQIVEAFVAYMSTT
                     DGKATIANESGIVSIAVTDVSWDDIKNQYPVCEQDNSDVTIYIGGSTSVENIARALTQ
                     EFSTKCGNFVAEHNHTGSGDAYKRTQGSESEGANLLHIGFASRYFKDTEPASVGTTGQ
                     ICYDAVVVVVNSQNNLLTSLTGEQLKQIFDGQINKWENLD"
     gene            complement(8074..8889)
                     /locus_tag="PLP02_04885"
     CDS             complement(8074..8889)
                     /locus_tag="PLP02_04885"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPF42810.1"
                     /translation="MKTEIETSLERIQSITELRPQIAVILGSGLGGFADLISEKKTAT
                     YKDIFPFWKSSVPGHSGKFHIGKFRGKPICCMEGRPHYYEGYSDEEMRVPVLLLQALG
                     IKTLIVTNACGGMNPEYIPGDIMLISDHINMTGRNPLAGMISKEGTSVFCDMSEPYDT
                     HLTKKAEKAASAQNIRTHTGIYVGYNGPSYETKAEINAYRRLGGDVVGMSTVPEVIIA
                     KWAGMKILGISCITNKATGLGEKKLSHQEVLEVANKTNDKLTKILTETINQID"
     gene            complement(8873..9733)
                     /gene="folD"
                     /locus_tag="PLP02_04890"
     CDS             complement(8873..9733)
                     /gene="folD"
                     /locus_tag="PLP02_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPF42811.1"
                     /translation="MAIIMDGKKLSKTIREKIAKETEILKIKYGKTPKLAVIVIGNDP
                     AGESYVAGKEKACRNVGIESVVYHKEETVSQAEMLELIGELNSDDEINGILLQMPVPR
                     HLNKDELVDKISPQKDVDGFTPENVANLTNSRPAMVPCTPLGIMELLEAYEINPEGKR
                     CVVIGRSQIVGKPVASLLLAKNGTVTICHSKTKDLPAVAREADILVAATGKPKMVDDT
                     FIKEGAAVIDVGISRVDGQLTGDVDFDKVEKKAGYITPVPGGVGPMTVACLLENTLLC
                     FKRMQNENGN"
     gene            complement(9735..10262)
                     /locus_tag="PLP02_04895"
     CDS             complement(9735..10262)
                     /locus_tag="PLP02_04895"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPF42812.1"
                     /translation="MYQEIKPYEYANEVTKKLPGGIFLTTNAAGKTNTMTIGWGGINI
                     IWGRPVFIVLVRDSRATYGLIEESNEFTVSVPMQKDMAKELAFCGTRSMRDCDKFKEL
                     NIALLPARKVSTPVIKEAGLHYECRVIYKQPLDKAAIPEFIKERYYGPTKPGNTCHTV
                     YYGEIVDQYIYKEGE"
     gene            10424..11269
                     /locus_tag="PLP02_04900"
     CDS             10424..11269
                     /locus_tag="PLP02_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPF42813.1"
                     /translation="MKIGFVGLGLMGGSIASALKKEHDILAYDVRKDSLDYALENHII
                     DRAFTDPKSLLAETDILFICLYPRDCVSFVKKWNSYFKPDCLVIEISGIKSGIIRDVS
                     LYVNGQFDMVFTHPIAGKEKSGVEYADKTIFLDQNFVIVPVASNQEKNLLLVENLARQ
                     MGFGNVVRIDSQVHDEIIAYTSQLAHVLALALIDSDDNQVTTSRFIGDSFRDLTRIAM
                     INDDLWSELMIENKTALLDKIEIMQNELSRYKQLIADGDYEGLRKAMKKSKAARIAIE
                     KDIKS"
     gene            11266..>12473
                     /gene="aroA"
                     /locus_tag="PLP02_04905"
     CDS             11266..>12473
                     /gene="aroA"
                     /locus_tag="PLP02_04905"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPF42814.1"
                     /translation="MKAVIYPGLPKGKVSIPASKSQTHRAVIAASLALGKSTISNISY
                     SEDIDATIGAMEKIGARFIRSADKLTVYGTSKIGFTEDNFVECNESGSTLRFLIPVFS
                     LCNQKVVFTGRRSLFQRPLGIYDSLFAKANSRFIKNDDSVIVAGSLVPGTYTLAGNVS
                     SQFISGLLFALPLLDGSSEIVVSEPFESRYYVDMTVSVLKLAGIIIEQENNRYLIKGN
                     QSYKHFDISLEGDFSQMAFFAVLGVLAGDVECKNMPETSLQPDRVVLNFISEAGGKYI
                     RTETGYRFLASSLEAITCNVGQSPDLAPVLSVMAAFSQGLSSITNAARLKIKESNRLE
                     AIWQIINGLGGKAELGNDYVNITGANELVGGSFNSFGDHRIVMSLAIAASRCQNPVLI
                     KNAEAVNKSYP"
BASE COUNT         3160 a         2864 c         2539 g         3910 t
ORIGIN      
        1 cgtagctttt actaaacggc tttattttgg ttttgtcttg gatcagactc atatcgattc
       61 cgtgcgtatg ataaaaaagt tcttcgccta ggacaccgaa tcgtttcttg agcttgtccg
      121 cgtcggatcc ggcaatatcc ccgatcttgt agaaacccat tcggttcaat ctggcttcca
      181 tgtttctccc gatgccccac attttgctta atggcgatac cggccacagt tgctcgggaa
      241 tgtcttcgta atcccatttg gcaacaaaat cctttttgtt ttttgatccg atatccagcg
      301 ccagtttggc cataagcatg tttggtccga tgccgcaact ggaatatatt ccggtttctt
      361 tgtaaattgt atccatcaat tctttggcta tttccaaatc gttcttgtga taatattcca
      421 gatagttggt gaaatccatg aacgcctcgt cgatcgagta tacatagatg tcatcatccg
      481 atatgtatct taaatagata ccgataatct gagcggaaaa agaaagatat gtctgcatcc
      541 gcggtttgcg gtaaatgata tccacgttct tgggcagttc gcgtatccgg catcttcccg
      601 gaactcccat tgttttcagg taaggcgaca cggccaaaac gatcgctccg ctgcctcttg
      661 ctttgtcagt gacgaccagc ggcgtcttaa atggatccaa gccaagaaga ctgcattcga
      721 ccgaagcaaa aaaacttttc aggtcgatgc agatgatatg cttgtgcagt ttgtattcgt
      781 ccatcatttt tcccacttcg ttcctttcat ctttattata ctattttccc ggtgataatt
      841 cagatgtgtt ccggtattta ttttcaaata tgaaaaacat ttcccgcatg tgttcgaggg
      901 aaatgttttg ttctatattg cgtcaaattt gttcaggaag aacaatagtt atttcggttc
      961 cttttccttc cttgctttcc aattcgattc ttccttggta aataagcact atatgtttca
     1021 cgatcgaaag tcccagtccg gttccgccgg actttttgct tcttgctttg tcaaccctgt
     1081 aaaaacgttc gaatacacgg ctttgatctg tacttggaat tccaattccg ttatcggtta
     1141 ctttgatggt aatctccttg tctgtcgcgt ttgtttcgat tttcacccat ccagattctt
     1201 tgccgtattg tatgctattt tccatcaggt tccttataag ttgttccata tgctcctgat
     1261 tggcactaaa ggtcatcggt acagattgat tgattagttt gatgtttttt gcttgtgcct
     1321 gcggttcgag tttgtccaca acctcgcaaa tcaccgcttg caaatcaatg gtcgacggcg
     1381 ctttaagcgg aatgtaattt tcgtatttag aaagatcaag catgtcaagc acaagtttat
     1441 tcatccgttt tgactcatcc agaatcctcg aggccaggtc ggcaatttca tcctggttgt
     1501 ggataatgcc ggaagtaatc aattcagaag aacccattat cgatgtcaac ggagatttga
     1561 gttcgtggga agcgttgacg aagaagtcgc gtttgagcgt ttccatattc ttgataatgg
     1621 taacatcagt gaatatcatc actacgccgg gttgattacg ccaatccttc tcttcatatg
     1681 cgactccaac ggagtagtat gaatcattga cgctaatgac taaagtcgtc gatccttttg
     1741 attcatatac tcggtcaacg gcctgttgaa gcggttgact acggaaaaga aagttgtaat
     1801 tgcggtagag atgattttct gaagaatagt tgaaaagatg ggcgagatac gagttgacca
     1861 gaacgatgtt ttcttgatag tcgagcatgc ataatccttg gttcatgtga tcaaggagaa
     1921 aatcgctttt ttgtttttct gcgttcagcg aaactatgtt atcggatatt gttttgttta
     1981 tttcgttgat ttccaccagt agcccattga tttcgtcata ttttgaaacc ggcaacagtt
     2041 cgttgtattt gccttctccg accgcttgca acactttttt caggtcgttg aacggagcca
     2101 tggttcggtc gataagcgat ttgcttaaaa aataagccag aaccgcgatg atggctgcta
     2161 tcaataaaag cagtccgatg aagtcgttga aaaacgggag aatcgaatta atggggaccg
     2221 acaccctaac ataatcgccg tcatcaaact ggaaagccag atacatcatc tgttggtcaa
     2281 gcgtaactga cttgcggatg tagattgttc cgggatcctg aaattccggc cgggtcaaat
     2341 gattttccaa ttcggttgcg gaagaatctg ccaggactat gcccgtatcg tccatgaatg
     2401 ttattcttag ataatcttcg atattgtcgt atttttcaat tatttctgcc ggcgtcaaat
     2461 cgttgtcgta gtcggtcttc actatctcta aatagtaccg caaattaaat tcggttacat
     2521 tgttcaagtt gcggcgaaca agaaatatcg aaccgaccac aaaagcaagc agtgcgacgg
     2581 aaacgacaag tatggactga gatgttattt tccttttcat aggatagcct tatagccgat
     2641 accgcgtaca gtaagaatcg gggcttccgc cagtcctatc gatgccagtt tttgcctgat
     2701 cgtcttgata tgggcatcga cggttctaga ttccccaata aaatcatagc cccaaacggt
     2761 ttcgacgatt tcgtttctag ttaccgtttg cccgttgtta gaaaccaaca gcttcaacag
     2821 ttcatattcc ttcgcggtca tgacgacgct tttgcctttg aatgtacacg ttctttcgct
     2881 atcgaaaaga attaggccgg aaacttcgat ttctttttct gtcccgcctt ttttgctgcg
     2941 gcgtagaagt gcttttatgc gactgacaag ttccaagacc ccgaacggtt tgatcatata
     3001 gtcgtccgct cccaaatcga gtccaaccac tttgtcaatt tccgaacttt tagccgacac
     3061 aatcagtacg gggatgtttt tccacataac ggtgtttttc attttctgta atattccaat
     3121 gccatcttct cccggcaaca taatgtcaag cgaaaccaga tcgggaagtt tttgttccat
     3181 ctgcgtgtaa aaactctcgg catcgccgaa gacctcaact tcaaaccccg aattttgcaa
     3241 agcgattttt ataacgtatt gaatatttat atcgtcttca acagtatata tcaaagatcc
     3301 cattttatag tctccttttc atatatcata attgtgcgct ccggttatgg caaatatcac
     3361 ccattcgccg atgttgacgg cgtgatcgcc aattctctcc aaatacttgg ctaccatcat
     3421 cagatatatc gcataatccg catccatcgt ttgcattctt atggctttga cgacatcgtt
     3481 tcttactttg tcgaacaaag catccacttc gtcgtcggat ttgataaccg cttttgctag
     3541 ttggatatcg agagaagtaa atgattccag cgcatctctg atcatgtcct gggtttttcc
     3601 ggttatttct gtcaggtcct cggtttttgt attttgtttt tccgtttcca tgaaaacggt
     3661 tatctttgct atgtcggcgg cgtgatcgcc gatcctttcc aagtcggtta tcatttttaa
     3721 aaccgacgtg atcagacgca aatcttttgc taccggctgt tcccgtaaaa tgattctgag
     3781 acactctttt tctatcatcg cctcgcaggc gttgacttct ttgtcctttt cgataatcat
     3841 tgtagccagt tcggtattca tgtcaagaaa ggatcgaaaa ctctcgtgaa tgttttcaat
     3901 caccaaatct cccattttca ccaaatctag tttgaggtgt tcgagttcgc tgtcgaactt
     3961 ggtcctttgc atcaaaggta tcatcctcca tttattatta taatataaag ttgtctttcc
     4021 ggccaaacga ttatccgaat ctgccggtga tataatcttc ggtttctttt tgtttcggct
     4081 ggccgaatat ctgatcggtt gtaccaaact cgaccaatct tcccaacaag aagaacgccg
     4141 tgtaatccga aacccgtgct gcttgttgca tgttgtgagt gacaatgaca attgtgtatt
     4201 tgtctttcag cccgacgatc agctcttcaa tcttcgccgt ggcaattgga tccagcgcgc
     4261 ttgtcggttc gtccatcagt atcacttccg gttccatggc gatcgtcctt gcaatgcaaa
     4321 gacgctgttg ctgcccgccg gaaagactca aggcattatc gtttaatctg tctttcactt
     4381 catcatacaa agccgcgtta accaatgcct tttttacaat atcctgcagt tccttttttc
     4441 cttttatgcc ttgacatctc ggaccataag cgacattgtc aaaaatactc atcggaaacg
     4501 gattgggttt ttgaaagacc attccgactt ttgtgcggag ttctatgaca tcataatttt
     4561 ttgtgtagat atcgttgttt tcgtatctga catatccttc tataaaacag ttgtcaatca
     4621 gatcattcat ccggttcaga catcttaata aagtagattt tccacatccg gatggaccga
     4681 taagcgcagt gatttttttt gcttcaatat ccattttgat gtttatcaaa gcttgtttat
     4741 ctttgtatct caaattcaat tcatcaattt caaatacttg ttccataaat attatcaacc
     4801 tcgattccga tcgaaagtca gtttataaga aatcaatttc actgtcatat tgagaatcaa
     4861 aacgacaatc aaaatgatta gcgaaacggc acaggcaagt tcgaagttgg gaacctcgcc
     4921 ggccatgatg ctccaaatga agacggcgag cgatgttgac ttttccgtaa ccactatgtt
     4981 gtcttttata acggttccta ttgcatagat caaagccgcc gattctccga tgatccttcc
     5041 cacactaagc aatgtcgctg ttaatatccc cggaagagaa cagcgtaata ttattttaaa
     5101 agtcgtttgg gttttattgg cgccgagggc aaacgatgcc tgccggtaat cgtcggggat
     5161 gactttcaag gattcttcca ccgatgaaat tatcaccggc aaaacgatta cggcaagagt
     5221 taaggatccg gatattatgt tgcctccgga cgctttggta agcgtactgg taagcggtat
     5281 gaaaacggca gcccccaaaa gaccgaatat gattgaagga acccctgtca acaattccac
     5341 cgatctccta agtacgttga tataccattt gtctttcgga gcgaattcgt gaagataaat
     5401 tgcagtgaaa acacctaacg gcatcgcgat tatcatggta aaaaatatca aatatccggt
     5461 tgtgacgagc gatcctctga ttccgcttcc ggcagttgtt gttatcatgt cttttatacc
     5521 gtcagcgttg tcaaaaagct ctatcgcagc ttttgctccg ttcttgctat acatgacggt
     5581 gttgttagaa aaaataattt ttaccatcga ttgaccgcca gatatggcca cataattttt
     5641 ttcatcattc ttgtcgcgca aattggccag cggagattca tccgatacat aaactacaag
     5701 gacgtagtct tttccttcaa aattgatgtc atcttttaag gctattcccc atttttcaga
     5761 gtagaaaacg tcactttcaa actctctgtt ggcggagtat ccgtcctgat tcagttctcc
     5821 ttcgtaatag ctgtcgtagg tggctgcgta ataatcgccc gtgatcaagt cccatgataa
     5881 cagattagct cctttatcga ttatgtacga gaacaaaagc atcaaaataa gcaagccaaa
     5941 cagtgaaatc gtggctattc ctgcatatgc tcctcgatca agaatatttt tcctcctttt
     6001 tttcatcatg aatatttccc aatccttttt ttgaaatgat tcaaaagcag attgccaaaa
     6061 agaatgacaa acaccagcac aatccccacg gaaaaacgaa tgtcatagtc gactccagat
     6121 gtttctttca aaccgagaag catggtggat gtcaaggttc tggttgtgga aaaaagatca
     6181 aaagaaggcc cgctcccggc gtttcccgca accatgctta cggctgtagc ttctccgagg
     6241 gctctcccga ttccaaggat gacaccggaa aatattcctg atttcgctgt taacaatgtg
     6301 actttgaaat atgtctggat tcttgatgcc cccaacgcga gagacccttt ttccagattg
     6361 gtatccacgg cgtttatcgc cgtgatcgac ataagtgtaa cggtagggta aatcattatc
     6421 gccaggacca gtatggtcga cagagtcgaa atgccaccgg ccgtctgaga cccaaacaca
     6481 tccgcgagtc cgattacgaa tttggttatg actccaagtc cgaaaacccc atagattatc
     6541 gaagggattg ccgccaacaa ttcaatgacg gttttcagaa atccgccgat gattctgttt
     6601 gtcatcttgg caataaatag tgccgtcgtg acggatatga ccaccgctat aatcgccgct
     6661 agcgccacaa catatattgt gttcacaaca atgaaaaaaa tgccataagc cgttggcgga
     6721 acataccatg tggttcctgt cagaaagcgc aaaagattta cactttcgct gccataactg
     6781 gaaacaagga aaggtgcgac ccctcgcttc gccacaaaga tgatgatgaa aaaaacgaag
     6841 gatgcgctga tgacagacgg aaccagcaat agaatgtgcg ttaacttatc ccggctgttt
     6901 ttctttttat ctatcgtcct gcaaaccatg ctaatccccc aaagtacaag cggcagaccc
     6961 gatcttcatg gtccgccgat tttttataaa tcccgttttc ttttcaatca agattctccc
     7021 acttgttgat ctgaccgtca aagatttgtt tcaattgttc gccagtcaaa gaagtcaaca
     7081 gattgttctg ggaattcaca accaccacca cggcgtcata acaaatttga cccgttgtcc
     7141 caacagaggc cggttccgta tctttaaagt atcttgatgc aaatccgata tgaagcagat
     7201 ttgctccttc cgattcactt ccttgagtac gcttgtatgc atctccggat cccgtgtggt
     7261 tgtgttcggc gacaaagttt ccacattttg tactgaattc ctgagtcaag gctctggcga
     7321 tattttccac ggaagtagat cctccgatat agatcgtaac atcggagttg tcttgttcgc
     7381 aaaccggata ctggtttttg atatcatccc acgaaacatc tgttaccgct atgctcacaa
     7441 tgcccgattc attggcgatg gtcgcttttc cgtcggtcgt tgacatgtat gccacaaacg
     7501 cttcgacgat ctgctgctct ctgtccatgc ccgtccaatc ggaacgaacg atgtaattga
     7561 acggacgctt gagtgcgtat gtttcgttaa tcacgttttc ttccgtcgct tcaattccgt
     7621 cgaacgctaa tcctgtcaat ccagattcca aaagtgatgc taacgaaata tatccgattc
     7681 cgtagatgtc gttcgttacg gcggtgatca tactgccgtt gctgtcgact tcaacatacg
     7741 aagttgctag aactgagttg tctgccaccg cgtcgtcaaa atctatcccg ctgaagaagg
     7801 cgtccctggt tccggatgtt gtgtctctgg tatacactgt gatatttctt gaactgtcaa
     7861 atgcttctgt agtagtgaca tccgatgtgt ttcccgcgca agcggtcatt gccgctacca
     7921 tgaatatcat tataccgaat aagatttttt tcattttctc tctccttttc atgcctttat
     7981 cttattccat ccaaggtttt tgaaaatacc gttgttaggt aaagttacgt aaattatatg
     8041 taaaaaaaag cacccttttc gggtgctgtc tcgttaatca atttgattga tggtttccgt
     8101 cagtattttt gtcaatttat cgtttgtctt gttggcgact tccaaaactt cctggtgaga
     8161 tagttttttt tctcccagtc cggtggcttt gtttgtaatg catgatatcc cgagaatttt
     8221 cattcccgcc cactttgcta tgataacttc cggaaccgtc gacattccca caacgtcgcc
     8281 gcctaatcgg cgatatgcgt tgatttccgc tttggtttca taactgggac cattgtagcc
     8341 aacataaatt ccggtatgtg ttctaatgtt ttgagcgctc gccgctttct cggctttttt
     8401 tgtgagatga gtatcgtatg gttcggacat gtcgcaaaac acgcttgtcc cttcttttga
     8461 aatcattccg gcaagcggat tccggccggt catgtttatg tggtcgctta tcaacatgat
     8521 atctccggga atatattccg gattcattcc tccgcacgcg ttggtcacga ttaacgtttt
     8581 gattccaagc gcctgcaaca acaataccgg aactctcatt tcctcgtcag aatatccttc
     8641 ataataatgt gggcggcctt ccatgcaaca aatcggtttg ccacggaatt tgccaatatg
     8701 gaattttcct gaatgccccg gtacggacga cttccaaaaa ggaaatatgt ctttgtaagt
     8761 tgctgttttc ttttcgctta tcagatcggc gaatccgcca agccctgaac ctagtatcac
     8821 agcgatctgt ggacgaagtt cggttatgga ttgaattcgt tccaaacttg tttcaatttc
     8881 cgttttcatt ttgcatccgt ttgaaacaaa gaagagtgtt ttccaagaga catgccacgg
     8941 tcatcggccc gacgcctccc ggaacggggg ttatgtatcc ggctttcttt tcgactttgt
     9001 caaaatcaac atcgcccgtt agttgcccgt cgactctgga tataccgaca tcaatcaccg
     9061 cggctccctc tttgatgaag gtgtcatcga ccattttagg tttgcctgta gccgcgacca
     9121 aaatgtctgc ctccctggcg actgctggaa gatctttggt tttgctatga caaatggtga
     9181 cggttccgtt ttttgccaaa agcagactgg caaccggctt ccctacaatt tggcttctcc
     9241 cgatcacaac gcatcttttt ccttctggat tgatttcgta ggcttccaaa agttccatga
     9301 tcccgagtgg agtgcaagga accatcgccg gtctgctgtt ggtcaggttg gcaacgtttt
     9361 ccggggtgaa accgtcaacg tctttttgag gtgatatttt atcgaccagc tcatctttgt
     9421 tcaagtgtct cggtacgggc atttgtaaaa gaataccgtt tatttcgtca tcagaattta
     9481 attcgcctat gagttcgagc atctccgctt gacttactgt ttcttctttg tgatatacaa
     9541 cgctttcgat gccgacgttc ctgcaggctt tttcctttcc ggccacgtat gattcccccg
     9601 cgggatcgtt tccgatcaca atcactgcga gttttggtgt cttgccatat ttaattttaa
     9661 gaatctcggt ttctttggca attttttccc tgatcgtttt ggataatttt ttgccgtcca
     9721 taataattgc catatcattc gccttcttta tatatgtatt gatcgactat ttctccgtaa
     9781 taaaccgtgt gacatgtatt tccgggtttc gttggtccat aatatctttc ttttataaat
     9841 tccggtattg cagctttgtc caacggctgt ttgtatatca ctctgcattc gtaatgcaat
     9901 ccggcttcct taattactgg agtcgatact ttccttgcag gaagcaaggc aatgtttaac
     9961 tctttaaatt tgtcgcaatc acgcatggat ctggttccgc aaaaagccaa ctctttagcc
    10021 atgtcttttt gcataggaac gctcacggtg aattcattgc tttcttcgat caggccatat
    10081 gtcgcccggg aatcccttac aagtacgatg aataccggcc gcccccaaat tatatttata
    10141 ccgccccacc cgattgtcat ggtgtttgtt ttaccggcgg cgttggtggt cagaaaaata
    10201 ccaccaggga gtttttttgt gacttcgttt gcatattcgt atggttttat ttcctgatac
    10261 atagtatcac ttcctttttt gattatacca taaaaagtct gtttcggcgt gtttttgatg
    10321 gtaccgtttt cgtttttgga tcaaattcca atattcttat ttttttatat tgtcagacct
    10381 tttttgtgat aaaattagat aaaatatgtt tggggtgcta tacatgaaaa tcggatttgt
    10441 tggtttgggc ttgatgggcg gttcaattgc atccgcctta aaaaaagaac acgatatcct
    10501 tgcatatgat gtccggaagg actctcttga ttacgccttg gaaaatcata tcatcgatcg
    10561 tgcgtttacc gatccgaaat ctctacttgc cgaaactgat attttgttca tttgtttgta
    10621 tccccgcgac tgtgtttctt tcgtcaaaaa atggaactca tatttcaaac ccgactgcct
    10681 ggttattgaa atctccggga ttaaatccgg gatcataaga gatgtatcct tgtatgtcaa
    10741 cgggcaattt gatatggtgt tcactcatcc gattgccggc aaagaaaaat ccggagtcga
    10801 atatgccgat aaaaccattt ttctcgatca aaattttgta attgttccgg ttgcttccaa
    10861 tcaggaaaaa aatcttcttt tagtagaaaa tctcgccaga caaatggggt ttggcaatgt
    10921 cgtccgcata gattctcaag tccacgatga aatcattgca tacacttcgc aactggcaca
    10981 cgttcttgcg ttggctttga tagattccga tgacaaccaa gtaacaacca gtcgtttcat
    11041 cggcgactct ttccgtgacc ttaccagaat agccatgatc aacgacgatc tgtggagcga
    11101 actaatgatt gaaaacaaaa ccgctctcct tgacaaaata gaaattatgc aaaatgagtt
    11161 gtcacgctat aaacaactga ttgctgacgg cgattatgag gggttacgaa aagcaatgaa
    11221 gaaatccaaa gcagcaagaa tcgccataga aaaggatatc aaatcatgaa agccgtcata
    11281 taccccggac ttccaaaagg aaaagtttcc atcccggcct ctaaaagcca gacacataga
    11341 gcagtcattg ccgcttcgct tgctttggga aaaagtacaa tatccaatat atcatacagc
    11401 gaagatattg acgctacgat cggggctatg gaaaaaatcg gcgccagatt catccgctcc
    11461 gcggacaaac taacagtcta cggaacttca aagatcggtt ttaccgaaga caacttcgtc
    11521 gaatgcaacg aatccggttc cactctacgt tttcttattc cggttttttc tctttgcaat
    11581 cagaaagtag ttttcaccgg aagaaggagc ctgtttcaga gacctttggg aatatatgat
    11641 tccttgttcg ccaaagcaaa ttcccgtttt ataaaaaacg acgattcggt tatcgttgcc
    11701 ggatccctcg ttcctggaac ttatacgctt gcgggcaatg taagcagcca gttcatatcc
    11761 gggctgctat ttgctcttcc tttgctggac ggaagttcgg aaatcgttgt ttccgagccg
    11821 tttgaatcgc gctactatgt cgacatgacg gtttcggttt taaagctcgc gggaataatt
    11881 attgaacaag agaacaatcg ttacctgatc aagggaaacc aatcatacaa acattttgac
    11941 atttctttgg aaggcgactt ttcccagatg gcatttttcg ccgttctcgg tgttctcgcc
    12001 ggagatgtcg aatgcaaaaa catgccggaa acttctctgc aaccagaccg tgttgttttg
    12061 aatttcataa gcgaagccgg cggtaaatat atccgtaccg aaaccgggta tcgctttctc
    12121 gcttcctcgc ttgaagctat cacgtgcaat gtcggacaat ctcccgatct tgctccggta
    12181 ttgtcggtta tggccgcctt ttcccaagga ctttcttcga taacaaacgc ggccagatta
    12241 aaaatcaaag agtcaaatcg tttggaagct atttggcaaa ttatcaacgg ccttggcgga
    12301 aaagcagaac tcggaaatga ttatgtgaac ataacaggcg caaacgaact ggttggtggc
    12361 tcttttaatt cctttggcga tcatcggatt gtcatgtcgt tggctatcgc tgcctcccgc
    12421 tgccaaaatc cggttttgat caaaaacgcc gaagcggtaa ataaatccta tcc
//
LOCUS       DAOPJL010000034        11769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_5726_length_11769_cov_9.295288, whole genome shotgun sequence.
ACCESSION   DAOPJL010000034 DAOPJL010000000
VERSION     DAOPJL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5726_length_11769_cov_9.295288"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLP02_04910"
     CDS             <1..530
                     /locus_tag="PLP02_04910"
                     /inference="COORDINATES: protein motif:HMM:NF023280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HPF42815.1"
                     /translation="KADETGYGVFEYYQGLIALRKAHSSFRMTSADDVINNLTFVYDN
                     VEGVVAYTITNGASNDEFDTIMVINNANEKAVKLELSGEEYWYLVTDGNIAGDEVLAA
                     FSGGTTIKIAAHSSCVLFQDSDPVQLPSETVKGLTVSVYNPLPVIIASIAGGVLLLAG
                     AGVFVVLKLRKRPEA"
     gene            677..1084
                     /locus_tag="PLP02_04915"
     CDS             677..1084
                     /locus_tag="PLP02_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF42816.1"
                     /translation="MDYEKSCGGVIYKEQNEEKYFLVVHMQMGHWTFPKGHVEPNETE
                     EETAVREIKEETNIDVELLSGFRKTTEYSPRPNAIKEVVYFIGRPVSEEISCQMSEVQ
                     EARWETLEKAKELLTYQSDKSILCLANHYLQLV"
     regulatory      1156..1328
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1370..1801
                     /locus_tag="PLP02_04920"
     CDS             1370..1801
                     /locus_tag="PLP02_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPF42817.1"
                     /translation="MKSLTIKEIKIGDAASLTKTITVNDVETFASTTGDMNPIHMDDE
                     YAKKTIFKQRIAHGMLVGSLFSPIFGVELPGLGSIYTKQSLKFIRPVNFGDTIIATVT
                     VKEIIPDRNRVVFECIAKNQNEETVVTGEAEIMPPLVRQDI"
     gene            1798..2442
                     /locus_tag="PLP02_04925"
     CDS             1798..2442
                     /locus_tag="PLP02_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006070638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPF42818.1"
                     /translation="MKEKINKDQFLDIAKDNAIVMIGGFLANGTPERLIDLLVDSNVV
                     DLTLICNDACYEDRGPGKLIANHQVKKLITSYVGNNKSVSDQVNEGLLVVELVPQGTL
                     AEKIRTGGAGLGGVLTPTGIGTLVQNGKKIIEIQGRDFLLEEPLFADIALIGGAVADK
                     YGNLRYIQTMRNFNPVMALAAETVICDPDEIVDTLDPECVVTPHPLVDYIFEEE"
     gene            2445..3092
                     /locus_tag="PLP02_04930"
     CDS             2445..3092
                     /locus_tag="PLP02_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPF42819.1"
                     /translation="MDDKNIIAKRIALELGKDELVDLGIGIPMLVSDYTDKEKNITFF
                     SENGIIGLGNLSADENTNLVNVDDRKATVVPGGSFIDSSQAFAIIRGGHIDVAVLGAL
                     EVDQEGHLASWIVPGERLSGMGGAMDLAVGARRLIVAMTHTDKGKPKIRKQCSLPLTA
                     LRSADLIVTEMAVIAVDKDGLYLKELHPDHTVEEVLNATEATLRVGEFSPMKGVI"
     gene            3092..3475
                     /locus_tag="PLP02_04935"
     CDS             3092..3475
                     /locus_tag="PLP02_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HPF42820.1"
                     /translation="MKTKLYLRMSSHDAHYGGDLVDGARILGLFGDAATELLIRNDGD
                     EGLFRAYEQVDFFHPVFAGDYLEVEAEIVHIGNTSRKMEFRCYKTVSPRKDISASAAD
                     LLKEKILVASAVGTCVVLAENQRNI"
     gene            3472..4293
                     /locus_tag="PLP02_04940"
     CDS             3472..4293
                     /locus_tag="PLP02_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPF42821.1"
                     /translation="MNKLVITCAITGAEVTKKDNPAVPYTIAETAESAYGAYKAGASI
                     IHLHAREDDGTPTQHWHRYNELIEAIKARCPGIIIQVSTGGSVGMTAEERMSPLEISP
                     EMASLDCGTLNFGKNDIFVNTISEIEMFAAKMHEKNVQYELGVFEKGHIDTALRLWKK
                     GIIREPLRFCFALGVYGGMTGEARDFEFMKGSIPEDALFSVAGVGKYEYILAEQAIIH
                     GGNVRVGLEDNLYIEKGVLASSNAALVEKAVWMAHSHGREIATPTEARMILKMVK"
     gene            4295..5335
                     /locus_tag="PLP02_04945"
     CDS             4295..5335
                     /locus_tag="PLP02_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPF42822.1"
                     /translation="MDNNSRYGIGRVISPKGVLPQAATRLDNNMVISDDEILVDVKTL
                     NIDSASFTQIAEVCHNNLQAIGDMVLSIVKERGKMQNPVTGSGGMFLGKVNQVGKALN
                     IDLKPGDEIASLVSLSLTPLRIDRIKSIHPEKDQIDIEGQAILFASGIYAKIPNDLPS
                     SLVLAALDVAGAPAQTAKLVKKGDTVLVIGASGKSGLLVSYQAKKSAGPEGKVIGIIN
                     DIRQLEDLQSINACDEIIKGDATNPLEIYEKVLSANGNEKVDVTINVVNVPNTEMTSI
                     LPTKDDGTVYFFSMATSFAKAALGAEGIACEATMIIGNGYTAGHADLTMDLFRESRTL
                     YDIFMQRYGSGE"
     gene            5337..6593
                     /gene="ablA"
                     /locus_tag="PLP02_04950"
     CDS             5337..6593
                     /gene="ablA"
                     /locus_tag="PLP02_04950"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPF42823.1"
                     /translation="MIKTNVSYLERKNKYFKTVSDADWNNWKWQVKNRIINAESLKKY
                     LDITPQEEKEINSVLNKFRMAITPYYLTLIDPDDPNDPIRKQAIPGIDELVVGKYDMN
                     DPLSEDVDSPVPGLTHRYPDRVLFLITDMCSMYCRHCTRRRFAGQLDNRLPQENIDKA
                     IEYIREHPEIRDVLLSGGDALLVPDEFLEDIIKRLREIDHVEIIRIGTRTPVVMPQRI
                     TDDLVMMLRKYHPIWVNTHFNHYNEITPESKAAIDKMADAGIPLGNQSVLLRGVNDSV
                     VTMRKLVNKLVEIRVRPYYLYQCDLSMGIGHFRTPVSKGIEIMEGLRGHTSGYCVPTY
                     VIDAPGGGGKIPVMPNYVISQAPGKIVLRNYEGVICTYAEPTDYVDDNESEPEEKTIG
                     VSSLLTGEETSIEPKVLQRRTRQLKH"
     gene            7082..>7577
                     /locus_tag="PLP02_04955"
     CDS             7082..>7577
                     /locus_tag="PLP02_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42824.1"
                     /translation="MDEVINDTERVIRRLSIPKSEKNLLTRNEWKKTEIFDFEGNWIG
                     AGEHSAVMDPEATLGLLGPGVGYLYVPGATTAEFVRKFIKTKDAGMSKLVVYSPTNLI
                     ADDFIDVFPKAAEGRIQTIRPVNIVALCYNPFSPAGYVFDDNEFYERLRTLTDLPIFN
                     VLSER"
     assembly_gap    7578..7677
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7678..8918
                     /locus_tag="PLP02_04960"
     CDS             <7678..8918
                     /locus_tag="PLP02_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPF42825.1"
                     /translation="YLLPSPKEADEILRPYYRKTFDIIQKNRRLREDQKTKLGVPKTQ
                     LYVIVATGNIYEDVVQAKAAATAGADIIAVIRSTGQSLLDYVPYGITTEGFGGTYATQ
                     ANFRIMRKALDDIGEKTGRYIQLVNYASGLCMPEIAAMAAVERLDVLLNDSMYGIIFR
                     DINMQRTFIDQYFSRLINAYAGITINTGEDNYLTTSDAVEEAHTVLASQFINEAFAIK
                     AHLPEKLMGLGHAFEIDPMVENSFLMEVAQAEMAREIFPDAPLKYMPPTKHITGNIFR
                     AYMQDTMFNMATVLTDQSIHLLGMMTEAIHTPFLADRFLAIDNAKLVQQAMKSAREEI
                     EYKPGGFVVTRAKTVLANAHAFLGEVEKINLFRALEAGMFAGVKRPYDGGKGLNGVIE
                     KQQGYFNVVADRMEAELNEN"
     gene            8908..9645
                     /locus_tag="PLP02_04965"
     CDS             8908..9645
                     /locus_tag="PLP02_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPF42826.1"
                     /translation="MKIRPYGDTMADGKVETSFTLPTTSDETGQEIARQFAKQMGFVD
                     PNIVHAEEIGEGFSFFVIYGSVPYTVDTDKIKVEKIETPTMTREEIEDLIEKEVGRDV
                     VFVGASTGTDAHTVGIDAIMNMKGFAGHYGLERYKHIRAHNLGSQVPNEEFVRQAKLL
                     GADVLLVSQTVTQKDIHLKNLENLVEILEAENMRNDVILIAGGARITHELAKELGYDA
                     GFGPKKYAEDVASYAITELIKRKNSKN"
     gene            9661..10194
                     /locus_tag="PLP02_04970"
     CDS             9661..10194
                     /locus_tag="PLP02_04970"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HPF42827.1"
                     /translation="MKISKLSQIATKKGDTGMSANYEGDLFPKSDRLFDVLGSLDECE
                     SILGICYHYGKNEDLKKIQKDLEKAAAQVASHPHENFSGRMDKLTVVDITWLELSAQT
                     MLDKKNLDPGFVLPGSETTLAGAYYDFARTVVRRCERSLWKFIEKNSRDDLNIVAGFV
                     NRLSDYLYVLARFEENS"
     gene            10294..10716
                     /gene="glmS"
                     /locus_tag="PLP02_04975"
     CDS             10294..10716
                     /gene="glmS"
                     /locus_tag="PLP02_04975"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HPF42828.1"
                     /translation="MEKRTSLKTIVIGVIGADVHAVGNKIIEYTLTNHGYNVINIGVL
                     SSQEDFINAAIEADAKLILVSSLYGQGEIDCRGMRDKCIEAGIGDILLYVGGNIVVGK
                     QDFREVKKRFLDMGFNRIYPPGTPIDKALCDIKKDLGE"
     gene            10718..>11769
                     /locus_tag="PLP02_04980"
     CDS             10718..>11769
                     /locus_tag="PLP02_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPF42829.1"
                     /translation="MARYLLIDFGSTFTKLTAVDTEKGDIIGTSAKATTVASDIREGY
                     HQALAALFAEIGHEVTFDKIIACSSAAGGLKMAAVGLVDELTVEAAKRVCLGAGAKVD
                     LVFSHHLTNHEVQEIVNQKIDIVLLAGGSDGGNTECVLYNANKLGEMKISVPIVFAGN
                     KTAEDDIKLIFQKYGMDGYICENVMPRINVLNVGSAKEKIREIFLKKIIAAKGIKKIE
                     EEIDRVVLPTPEAVLQASELLSRGYMHEPGLGDIVIVDIGGATTDMYSMCLATNRSDV
                     ILHGLEEPFAKRTVEGDLGMRYSAPGIVKNLSKEEIRLIDEEKGIDIETEVAYRFHNV
                     WSLPENEHDEHIDELLA"
BASE COUNT         3528 a         2425 c         2908 g         2808 t
ORIGIN      
        1 agaaagccga tgaaaccggg tatggcgttt ttgagtatta ccaaggtttg attgccttga
       61 gaaaggccca ttcgtcattc cgcatgacca gcgcggacga tgtaatcaac aacctgacat
      121 ttgtttatga taatgtggaa ggcgtagttg catacaccat aaccaatggc gccagcaatg
      181 atgagttcga taccataatg gtgattaaca acgctaatga aaaagcggtc aaactggaac
      241 tttccggcga agaatactgg tatctcgtca ccgatggaaa tatagccgga gacgaagtgc
      301 ttgcggcatt ttctggcggc actactatca agatagccgc ccattcgtct tgtgtcttat
      361 tccaggattc cgatccggtt caacttccat cagaaacagt taagggattg actgtcagtg
      421 tatataatcc gcttccggtg ataatcgctt cgatagcagg cggcgtctta ttattggctg
      481 gagccggcgt gtttgtcgtt ctgaaactcc gcaagagacc ggaagcctaa gagtaaattt
      541 ggtttttctt tcttagtcga gattatcggg aatcaattgc ccgtggcaac acaataatgg
      601 cgatcggtat tcgtaccatc gcctttttgg ggccattaag gcaaaaacaa tagatacttt
      661 gatggaggaa tcgaagatgg attacgaaaa atcttgtggc ggtgtgattt acaaggaaca
      721 gaatgaagaa aaatattttc tcgtggttca catgcaaatg ggccattgga cttttccaaa
      781 agggcatgtc gaaccgaatg aaaccgaaga ggaaaccgca gttagggaaa tcaaggaaga
      841 aacgaacatt gatgtggaat tgctgagcgg gttcaggaaa acaacggaat attccccgcg
      901 gccgaatgcc attaaagaag tggtatactt tatcggccgt ccggtaagcg aagagatttc
      961 ttgccaaatg tccgaggtgc aagaagcgag atgggaaaca ctggaaaagg ccaaggaatt
     1021 attgacgtat caaagcgata aaagcattct ttgcctggca aatcactatt tgcaattggt
     1081 ttgatcaaaa gaccgtttga aacggtcttt tcattattga caacgcttac attattgggt
     1141 ataattatta cgaactgaat acacagaggc gcgatacgcc ggtatgaagc cgatgccaat
     1201 gggataggcc ccaaacgcgg cggaagaatg ggcgtgtcgc cgaaagaata taccggtcta
     1261 tcggtaaatt cttggtatgg tggccaaagc cttcgtactg ccatcgctga tggagagctg
     1321 ttatatattt gatataacgg ctttttttat gaaacaagga ggagttatta tgaaaagtct
     1381 gaccatcaag gaaataaaaa tcggagacgc cgcttcgctt acaaaaacga taactgttaa
     1441 cgatgttgaa acatttgctt ctacaaccgg tgatatgaat ccgattcata tggacgatga
     1501 atatgccaaa aaaacgattt tcaaacaaag gattgcccac ggaatgttag tgggatcgct
     1561 tttttcaccg attttcgggg tggaactacc aggactcgga agcatatata cgaaacaaag
     1621 tttgaaattc atcagaccgg tgaatttcgg tgatacgatt atcgccaccg taacagtaaa
     1681 agaaatcatt cccgaccgca atcgagtcgt tttcgaatgc atcgctaaaa atcaaaatga
     1741 agaaacggta gtgaccggag aagccgaaat aatgccgccg cttgtgaggc aggacatatg
     1801 aaggaaaaaa tcaacaaaga tcaatttctt gatatcgcca aagataatgc gattgtcatg
     1861 atcggaggtt ttttggcaaa cgggacgccg gaaagattga tcgacttgct tgttgacagc
     1921 aacgttgtgg atcttactct gatatgtaac gatgcttgtt atgaagaccg cggacctggt
     1981 aaactgatcg ccaatcatca agtaaaaaaa cttatcactt cttatgtcgg aaataataaa
     2041 tcggtgtccg atcaggtaaa tgaaggcttg ctggttgtcg agctggttcc gcaaggcacc
     2101 ctggcggaaa agattagaac cggcggagcc ggactcggtg gagtattgac accgaccgga
     2161 atcggcactt tggtacaaaa cggaaaaaag atcattgaaa tccaaggccg tgattttcta
     2221 ttggaagagc cgctgtttgc agatatcgct ttgatcggcg gagcggtggc ggacaaatac
     2281 ggaaatctca gatacatcca aacaatgaga aatttcaatc ccgtgatggc attggctgcc
     2341 gaaacggtaa tatgcgaccc tgatgaaatc gtcgatacat tggatccgga atgcgttgtc
     2401 accccgcatc cgcttgtcga ttacattttc gaggaggaat aaagatggac gataaaaaca
     2461 tcattgccaa acggatcgcc ctcgaacttg gaaaagacga actggttgat ctgggaatcg
     2521 ggattccgat gcttgtgtcc gattataccg ataaagagaa gaacatcact tttttttcgg
     2581 aaaacggaat catcggattg ggaaatcttt ccgccgatga gaataccaac ttagttaatg
     2641 tagatgatcg caaagccact gttgttcccg gaggatcttt catcgattca tcccaggctt
     2701 ttgccattat acggggagga cacatcgatg ttgccgtcct gggcgctttg gaagtggatc
     2761 aggaagggca tttggcttca tggattgttc cgggcgaaag attgtcagga atgggcgggg
     2821 ccatggattt ggcggtagga gccaggcgat tgattgtagc aatgacgcat accgataaag
     2881 gcaagccgaa aatccgcaag caatgcagtc ttcccttgac ggctcttcgt tcagccgatc
     2941 ttatagttac ggaaatggcg gttatcgccg ttgacaagga tggtttgtat ctgaaagagc
     3001 tccatcccga ccacactgtt gaagaagtgc ttaacgccac cgaagcaacg cttcgtgtag
     3061 gcgaattcag ccccatgaaa ggagtcatat aatgaaaaca aaattgtact tgagaatgtc
     3121 atcccatgac gcccattacg gaggcgatct ggtcgacgga gccaggatcc tcggtctttt
     3181 cggagacgca gcaacagaat tgcttatcag aaacgacggc gacgaaggtc ttttccgcgc
     3241 ctatgaacag gtggactttt tccatccggt atttgccggg gattatctgg aagttgaggc
     3301 cgaaatcgtt catattggca acacatcgag aaaaatggaa ttccgttgtt acaaaacagt
     3361 ttcacccaga aaagacattt cggcgtccgc tgccgacttg ttaaaagaaa aaatcctagt
     3421 ggcatcggct gttggcacct gtgtggtctt agccgagaat cagaggaata tatgaataaa
     3481 ttagtgataa cttgcgccat taccggcgcc gaagttacta aaaaggataa tccggcagtt
     3541 ccatatacca tagcagaaac cgcagagtcc gcatatggcg cttataaagc gggagccagc
     3601 ataatacatt tgcatgccag ggaggatgac ggaactccga cccagcattg gcatcgttat
     3661 aatgagttga tcgaagcgat caaggcaaga tgcccgggta tcatcatcca ggtatcgacc
     3721 ggaggttcgg tcggaatgac ggcggaagaa agaatgtctc cgttggagat tagtccggaa
     3781 atggcgtcgc tagattgcgg cacccttaat tttggcaaga acgacatctt tgtgaatacg
     3841 atcagcgaga ttgaaatgtt cgccgccaaa atgcatgaaa agaacgtaca gtatgaactg
     3901 ggtgtatttg aaaaaggaca tatcgacacc gctttgcggc tttggaaaaa agggattatc
     3961 agggaaccgc tccgattctg ctttgccctt ggcgtctacg gaggcatgac aggcgaagcc
     4021 agagatttcg agttcatgaa aggaagcatc cccgaagatg cgcttttttc tgttgctgga
     4081 gtcggaaagt atgaatatat tttggcggaa caggcaatca ttcacggtgg caatgtcaga
     4141 gtcggtcttg aagacaactt atatatcgaa aaaggcgttc tcgcctcatc gaatgcggcg
     4201 cttgtggaaa aagccgtatg gatggctcac tcccacggcc gggagatagc cactcccaca
     4261 gaggcaagaa tgatcctgaa gatggtgaaa tgatatggac aacaattcca gatacggaat
     4321 cggacgtgta atcagcccga aaggtgtctt gccgcaagcg gccacacgcc tggacaataa
     4381 tatggttatt tcggatgacg aaattcttgt cgatgttaaa acccttaata tcgactcggc
     4441 ttctttcacc cagatagcgg aagtttgtca taacaacctt caagcgattg gcgacatggt
     4501 tctttccatt gtcaaagaaa gaggaaaaat gcaaaatccg gtcactggca gcggcgggat
     4561 gtttcttggc aaagtcaatc aagtaggcaa ggctttgaat attgatttga aaccgggcga
     4621 tgagatcgct tcgcttgtat cattatccct gacaccacta aggatagata gaataaagag
     4681 cattcatccg gaaaaggatc agatagacat tgaaggacaa gccattcttt ttgcctcagg
     4741 aatatatgca aagataccaa acgatttacc aagcagtctt gtgttggccg cgcttgatgt
     4801 tgccggagca ccggcccaaa cagccaaact ggtcaaaaaa ggcgataccg ttcttgtgat
     4861 cggagcctcc ggaaaaagcg gactacttgt gtcttatcag gcaaaaaaga gtgcgggtcc
     4921 cgaaggaaaa gtcatcggca tcatcaacga tatcagacaa ctcgaggatt tgcaaagcat
     4981 caatgcgtgc gatgagatca ttaaaggcga tgccaccaat ccgttggaga tctatgaaaa
     5041 agtcttatct gccaacggca atgagaaagt cgatgtgacg atcaatgtcg tgaatgttcc
     5101 caatacggaa atgacttcga tacttccgac gaaggatgat ggaacagtct atttcttttc
     5161 catggcgact tcttttgcca aagcagctct cggtgcggaa gggatcgctt gcgaagccac
     5221 gatgatcatc ggcaacggat acacggcagg acatgccgat ctgacaatgg atctgttccg
     5281 ggaatctcgg accttatatg acatcttcat gcaaagatac ggaagcgggg aatgaaatga
     5341 ttaagacaaa cgtaagttat ttggaaagga agaacaaata tttcaaaacg gtatcggatg
     5401 cggattggaa caactggaaa tggcaggtta aaaaccggat tataaacgcc gaaagcctaa
     5461 aaaaatatct ggacataacc ccacaggaag aaaaagaaat caactctgtc cttaacaaat
     5521 ttcggatggc gatcacgcct tattatctga cattgatcga cccggatgat ccaaatgatc
     5581 ctattcgcaa acaagcgata cctggcatag atgaactcgt cgtcggaaaa tatgatatga
     5641 acgacccctt gagcgaggat gtcgattctc cggttccggg attgacacac cgttatccag
     5701 accgtgttct ttttcttatc acggatatgt gcagtatgta ctgccggcat tgcactagaa
     5761 gaagatttgc cggccaactt gacaacagac tgccacagga gaatatcgat aaagcgatcg
     5821 agtatatcag ggaacatccg gaaattcgtg atgtcctttt atccggtggc gacgctttgc
     5881 tcgttcccga tgaatttttg gaagacatca tcaaaagact acgcgaaatc gatcatgttg
     5941 aaatcatcag aatcggaaca aggacaccgg ttgtaatgcc ccaaagaata accgacgatt
     6001 tggttatgat gctgagaaaa tatcatccta tctgggtcaa tacgcatttt aatcattata
     6061 acgaaatcac tccggaaagc aaggcggcca tcgacaaaat ggcagatgcc ggaattcctt
     6121 tgggaaacca gagcgttctt ttgcgcggag taaacgactc ggttgttacg atgcgcaagc
     6181 ttgtgaacaa gctggttgaa ataagggtaa gaccatatta tctttatcaa tgcgatctgt
     6241 cgatggggat cggacacttc cggacaccgg tttccaaagg aatcgaaatc atggaaggcc
     6301 tacgcggcca taccagcgga tactgtgttc cgacatacgt gatcgatgct ccgggaggcg
     6361 gaggaaaaat tccggtcatg ccgaattatg ttatttcgca agcccccggc aaaatcgtac
     6421 taagaaacta tgaaggtgtg atttgtactt acgcagagcc caccgactat gttgatgaca
     6481 acgaatcaga acccgaagaa aaaacgatcg gcgtttcttc tttgcttacg ggagaagaaa
     6541 catccattga accgaaagtt ttgcaaagaa ggacaaggca attaaaacat tagtttcctt
     6601 gattgaaccc tacgatgtcg taagcatcat cgggaacgcc aagaatgccg gcaagacaac
     6661 agtgttgaat tatcttctgg aaagaatgaa aaaaaaggtc attggagtat cttccatcgg
     6721 tatggacggg gagcccctgg ataatgtttc ctttcttccg aagccgagga tacatttatg
     6781 gccgggaaca cttgtcgcta ctgcggaaat gtttctggaa acggcgacaa ttggcgaaaa
     6841 aatactaaaa agaaccgata tatccacgcc ttttggcaat attgtggtcg cagaggccgt
     6901 aaaaccggga aatatattgg tcgcaggtcc gtccacaatt gccgacatgg aaaaaatcgt
     6961 gcatattttt catgaacacg gcgcacaaaa aaaatttata gatggggctt tcgcgcgcca
     7021 aagtacggca aaaatggctg atggaacgat tcttgtttcc ggcgcaaaca ggtctccggt
     7081 catggatgaa gtaataaacg acaccgagag agtcataaga cggctttcca tcccgaaatc
     7141 cgaaaaaaat ctattgacga gaaacgaatg gaaaaaaaca gaaattttcg attttgaagg
     7201 caactggatc ggcgcaggcg aacattccgc agtgatggat cccgaagcca cactcggttt
     7261 gctgggtcct ggtgttgggt acctttacgt accgggagcg acgacagcgg agttcgtaag
     7321 aaagttcata aaaacgaagg atgccggaat gagcaaactg gttgtctatt cgcctaccaa
     7381 cttgattgcg gatgatttta tcgacgtgtt ccccaaagcg gcggaaggcc ggatccaaac
     7441 aatcagaccg gtaaacattg tcgctttgtg ttacaatcct ttttcacccg ccgggtatgt
     7501 tttcgatgac aacgaatttt atgaaaggct gagaacattg acggatttac cgattttcaa
     7561 tgtcctttcg gaaaggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct
     7681 atttgttgcc aagtccgaag gaagccgatg aaatcttaag gccatattat cgcaagacat
     7741 ttgatatcat tcaaaaaaac cgtcggttga gagaagacca aaaaacaaaa ctgggggttc
     7801 ccaaaaccca actttacgtg atagtcgcga ctggaaatat atacgaagat gtcgtacaag
     7861 ccaaagcggc ggctacggcc ggagccgata taattgccgt tatcagatct acgggacaat
     7921 cgttattgga ctatgtgcct tatggaataa cgacagaagg tttcggagga acatacgcca
     7981 ctcaggccaa cttccgtatt atgagaaaag cgcttgatga cataggcgaa aaaacaggaa
     8041 gatatatcca actcgtcaat tatgcttccg ggctttgcat gcctgaaatt gccgcgatgg
     8101 ccgccgttga aagactggat gtactattga atgacagtat gtacggaatc atatttcgcg
     8161 acatcaacat gcaaaggaca ttcatcgacc aatatttttc ccggttgata aatgcttacg
     8221 cgggaataac tatcaatacc ggtgaagaca attatctgac aaccagtgac gctgtcgaag
     8281 aagctcatac ggttctcgct tcccaattca ttaacgaggc tttcgccatt aaagcgcatc
     8341 tgccggaaaa actgatgggg ctcggacatg catttgaaat tgatccgatg gtggaaaaca
     8401 gttttctgat ggaggttgcc caagccgaga tggcgaggga aatattcccg gacgcaccgc
     8461 ttaaatacat gccgccaacg aagcacataa ccggaaatat ttttcgggct tatatgcagg
     8521 atacaatgtt caatatggct acggttctta cggaccagtc gattcatctt ttaggcatga
     8581 tgaccgaagc gatccatacg cctttccttg cagatcggtt tttggcgatt gacaatgcca
     8641 agcttgtcca gcaagctatg aagtcagcaa gagaggaaat cgagtataag ccaggaggct
     8701 ttgtggtaac cagggcaaaa acggttttgg ccaatgctca tgcattttta ggcgaagtgg
     8761 aaaagatcaa tttattcaga gctttggaag ccggaatgtt cgccggtgtc aaacggcctt
     8821 atgatggtgg aaagggtttg aacggagtaa tcgaaaaaca acaaggctac ttcaatgttg
     8881 tggctgacag aatggaggcc gaattgaatg aaaattagac catacggtga caccatggcc
     8941 gatggcaagg ttgagacaag tttcaccttg ccgacgacgt cagacgagac aggccaggaa
     9001 atagccagac aattcgccaa gcaaatgggg tttgttgatc cgaacatagt gcacgccgaa
     9061 gagataggag aagggttcag ctttttcgtg atttacggat cggttcctta tactgtcgat
     9121 accgataaaa taaaagtcga aaagatcgaa accccgacaa tgacaaggga agaaatcgaa
     9181 gatttgatcg agaaagaggt cggacgcgat gtcgtttttg tcggagcttc caccggaacc
     9241 gacgcccata ccgtcggaat tgatgccatc atgaatatga aaggatttgc cggtcactac
     9301 ggccttgagc gttacaaaca catccgggcg cacaatcttg gcagtcaagt accgaacgaa
     9361 gaatttgtga ggcaagccaa actacttgga gccgatgttc ttctagtcag ccagacagta
     9421 acgcaaaaag atattcattt gaagaatctg gaaaatctgg tggaaatact agaggcggag
     9481 aatatgcgaa atgatgtcat cctgatcgcc ggcggcgcga gaataaccca tgaactggca
     9541 aaggaattgg gatatgacgc tgggttcgga ccgaaaaaat atgccgagga tgtggcatca
     9601 tatgccatta ctgagctaat aaaacggaag aattccaaaa actgagtcgg gggcgaaata
     9661 atgaaaataa gcaaactgag tcaaatcgca accaaaaagg gagatacggg gatgtcggcc
     9721 aattatgaag gcgacctgtt tccgaaaagc gatcgtttgt ttgacgtttt gggttcgctt
     9781 gacgaatgcg aaagcattct cggaatttgt tatcattacg gaaaaaacga ggatttgaaa
     9841 aaaatccaga aagatctgga aaaagcagca gctcaggttg cctcgcatcc gcatgaaaat
     9901 ttttccggac gaatggataa attgactgtc gttgacataa catggcttga gttgtcggcg
     9961 caaaccatgc tcgataaaaa aaaccttgat ccgggctttg ttctgccggg aagcgagaca
    10021 acgcttgccg gtgcttatta tgactttgcc aggaccgttg ttcgtcggtg cgaaagaagt
    10081 ctgtggaaat ttatcgaaaa gaatagccgg gatgatctca acatagttgc cggatttgtc
    10141 aaccggttga gtgactatct ttacgtgctc gcccgtttcg aagaaaacag ctgatttatg
    10201 gaccgatcgc attagttgac aaaaccccca attatatttt gaaacttact attatgtatg
    10261 atataatgtt ggcgtaaaaa aacgctttca tgtatggaaa agaggacttc attgaaaaca
    10321 atcgtgatag gcgtcatcgg cgccgatgtg catgcggtag gaaacaaaat cattgaatac
    10381 actttgacaa accatggata caatgttatc aatatcggcg ttctttcatc gcaggaagat
    10441 ttcatcaatg ccgccatcga ggccgatgcc aagttaattt tggtttcatc gctttatggt
    10501 cagggagaaa tcgattgtcg tggtatgaga gacaaatgca tagaagccgg gattggtgac
    10561 attttacttt atgtcggcgg aaacatcgtg gtcgggaaac aagatttccg ggaagtcaaa
    10621 aaaagatttc ttgatatggg attcaatcga atttatccgc ccggaacgcc gattgacaaa
    10681 gccctttgtg atataaaaaa agatttagga gaatgaaatg gctcgttatc ttttgatcga
    10741 cttcggatcc accttcacca aactgacagc tgttgatacc gaaaaaggag acatcatcgg
    10801 cacatctgcc aaggcgacga cggtcgcctc cgatatcagg gaaggatacc accaggcttt
    10861 ggcggcgctt tttgccgaaa tcggacacga ggtcacattt gacaaaatca ttgcctgttc
    10921 atcggcggcc ggcggattga aaatggcagc cgttggccta gtcgacgaat tgacagtaga
    10981 agcggcaaag cgggtttgtc ttggcgccgg cgccaaagtc gatctggttt tttcccatca
    11041 tctcaccaac catgaagtgc aagaaatcgt caatcagaaa atcgacatag tattattggc
    11101 tggcggatct gacggcggaa acacagaatg cgttttgtat aacgccaaca aacttggaga
    11161 aatgaaaata tctgtaccga tcgtttttgc cggaaataaa acggcggagg acgatatcaa
    11221 actgatattt cagaaatacg gcatggatgg atatatatgc gaaaacgtca tgcccagaat
    11281 caatgttttg aatgttggca gcgccaaaga aaagattcgg gaaatctttt tgaagaaaat
    11341 cattgccgcc aaaggaataa aaaaaatcga agaagagatc gaccgcgtgg ttttaccgac
    11401 gccggaagca gtgcttcagg catcggaact gctttcccgc ggatatatgc atgaacccgg
    11461 actcggagat atcgtcatag tagatatcgg tggcgccacg accgacatgt attcaatgtg
    11521 cttggcgacc aaccgaagcg atgtcattct tcacggactg gaagaaccgt ttgccaaacg
    11581 tactgtcgaa ggcgacctcg gcatgcgcta ttccgcaccc ggtatcgtca aaaatctttc
    11641 taaggaagaa atccggctta tcgatgaaga gaaaggtatc gacatcgaaa cggaagtggc
    11701 ttatcggttt cataatgttt ggtcgcttcc ggaaaacgag cacgacgaac atattgacga
    11761 actgcttgc
//
LOCUS       DAOPJL010000035        11365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_5982_length_11365_cov_8.244916, whole genome shotgun sequence.
ACCESSION   DAOPJL010000035 DAOPJL010000000
VERSION     DAOPJL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5982_length_11365_cov_8.244916"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLP02_04985"
     CDS             complement(<1..370)
                     /locus_tag="PLP02_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPF42830.1"
                     /translation="MAEYKYDAWGKTIYINSYNLNIANVNPFRYRSYYYDYETGWYYL
                     QSRYYDPTIGRFISADGMVGAVGDILSANMYAYCANNPIMFTDETGYSWRSFWDDVGD
                     WFDETFGFSLYKEENAYESYY"
     gene            complement(716..2143)
                     /gene="glnA"
                     /locus_tag="PLP02_04990"
     CDS             complement(716..2143)
                     /gene="glnA"
                     /locus_tag="PLP02_04990"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPF42831.1"
                     /translation="MTKNMVGIKEFIKENNIEMVDFKMIDLDGRWRHLTIPASRFQEN
                     IFRYGIGFDASNYGYAPIEKSDMIFIPDPTSATIDPFAQAKTLAMIGDVYVIGETNEP
                     FGQYPRNVSRRAIEFMRKSGVADEMIIGPEFEFHIFDKAEYKLMPGDVYYHLESNRAS
                     WSHSARQSGDGYMLAADAGYHADIPQDADYDLRSRMSLELEKLDIAVKYHHHEVGGSG
                     QHEIEVELGNMEKMADDTMMIKYVVKNSALRAGKTATFMPKPIRGEAGNGMHVHMLLK
                     KDGEPLFYDKDGYAQLSQIAMYFMGGILSHISSLSAFTNPSTNSYRRLTPGFEAPVVV
                     GFATANRSAIIRVPAYAKTPSVKRFELRNPDATANPYYAYSAILMAGLDGIEKKIDPA
                     AGNWGPFDFNLYSLSEDERGKIDKIPTSLIEAIDALESDHDYLTKSGVFPEELIKVWC
                     ERKRREAREISEIPHPAEFIRYYDI"
     gene            complement(2285..3670)
                     /gene="amrA"
                     /locus_tag="PLP02_04995"
     CDS             complement(2285..3670)
                     /gene="amrA"
                     /locus_tag="PLP02_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPF42832.1"
                     /translation="MPLLKAYIMPHPPLAVPAIGGNEIEKIGKTIAAFEKVAEEIAQL
                     APETIIVSSPHSIMYSDYIHISPGNFATGDFRLFGVKSVGMAKEYDDELVEHIVREAK
                     ECNLRAGTLGERDKSLDHGTMVPLYFIEQKYADYKSVRMSISGLSLYDHYEFGKCVRK
                     AIEETNRRVVYIASGDLSHKLSADGPYGFSPDGPIFDQDVTLAIAKGDFLKFLTFSES
                     FLESAAECGLRSFVIMAGLFDKEKVTPKLLSYEGPFGVGYGIASFEPAGADSTRDFDK
                     QAREAETKEISKTREREDIYIRLARMSLENYVLTGKRLILPEGLPDELVRKQAGVFVS
                     LHEHGRLRGCIGTIMPQSKSVAEEIIKNAVSAGTNDPRFDKVEATELKDIIYSVDVLE
                     LPERIASKAELDPKKYGVIVRSGYRSGLLLPNLDNVDTVEDQVNIVLRKAGIGSGENY
                     SMERFQVTRHE"
     gene            complement(3672..4019)
                     /locus_tag="PLP02_05000"
     CDS             complement(3672..4019)
                     /locus_tag="PLP02_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42833.1"
                     /translation="MSIRDDVFRAYCGGDVESVLSNVKTMKKAGIHLEVTTLVVPGVN
                     DSDSELTKMANFIAEKLGKDTPWHISRFFPAWRMDEGEPTPATTLKRARAIGENAGLE
                     NIHIGNIREWEMN"
     gene            4031..4864
                     /locus_tag="PLP02_05005"
     CDS             4031..4864
                     /locus_tag="PLP02_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF42834.1"
                     /translation="MIDSSFAESHGYNIGDTIQIELTAYSLSFETQITGTMCYAEATS
                     TKSVCPIYISIAQNQGLLPFFNAYANQALVKTGDVAYTRQAINNHYSDQNNLLFILDM
                     DTTESFTLLENEVSQSKAMIYVFPVIFLFVSVLVILTTISQLILRERMNIGTLKALGY
                     SNPEIASHYSSVGIIVTLFGALIGVMIGPKIIPHVMEVKYELVYNLPAYDGVIYSILW
                     SFAAILAVGLLSASLSFLICRDVLKETPAQCMRPKAPKNMMMIRLIGGDGHDEPVAPV
                     D"
     gene            4836..5918
                     /locus_tag="PLP02_05010"
     CDS             4836..5918
                     /locus_tag="PLP02_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42835.1"
                     /translation="MMNQSRLSIKMAIRNIIVHPGRAFMSVIGVMGCVALLVCAFGIG
                     DSVNYSIDNELNRQFTYDINTTYDKANETVLYDYLDNKGADYEPYRSFLATVSGYSEA
                     SIYVYVLESPSAHTTIDVSEGVAISRSKADTIGVKEGDEITISVASGSVVVTIDSLVD
                     TSVTQGVFISEADFGLIDLDAILTYHLWISLYGATDEIVDEINSINGTNGAWLKSDRQ
                     AMIDDKVTSINTIRKTMMIFSILLSVVVLYNFSLLNMKESIRDIATLKVLGHKNIRIG
                     KILVYQMMILVSIGTIFGLLLGYPMLYLVMSSNKVDVMAYLYHIEPASYVYAALLSIG
                     TGFIFNIVFTTYIRKIKMVESLKSIE"
     gene            5943..6422
                     /locus_tag="PLP02_05015"
     CDS             5943..6422
                     /locus_tag="PLP02_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42836.1"
                     /translation="MIWRHIKVVVLFTFVVMLGILAFIPLPDFPATGFYETFETIYGD
                     EIADKNLKYLAFDETSLPIGYRPYFNTLMRQYCRRNHMRYLIATYDELKEDGYIIDGG
                     FQNGVILHFHNISMTGNDNISGEISIYSGSLSATGRDFFTQYDDGWIVNTSNSFWIS"
     gene            6616..7434
                     /locus_tag="PLP02_05020"
     CDS             6616..7434
                     /locus_tag="PLP02_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPF42837.1"
                     /translation="MYEIIEKKPQNADVDLLEILAPMVAKNAKPGQFLIIRVDKNGER
                     IPMTIVSHTETTVTIIVQKVGYSTMLLGKMQKGDALEDVVGPLGMPTIIRPVKKVLGI
                     AGGVGAAPLYPQLREYASRGSSVDLIIGARNRDHLILKDDFAAFCENIHIATDDGSIG
                     TKGFVTDVAKRLYETETYDLVIAIGPLAMMRAVVELDKKHGLKTDVSLNPIMIDGTGM
                     CGNCRVSIAGKTYFACVDGPDFPGDDIDFDELLARQNSYREEEHICRIKLGDKK"
     gene            7431..8804
                     /gene="gltA"
                     /locus_tag="PLP02_05025"
     CDS             7431..8804
                     /gene="gltA"
                     /locus_tag="PLP02_05025"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPF42838.1"
                     /translation="MIKDRIIMQTQDPLIRNHNFNEVALGFSENEAILEASRCLQCKN
                     PTCIAACPASVKIPDFIRLIKEKNSRVAYDTIYKDNVLPAVCGRVCPQESQCEGACVL
                     NRTGRPIAIGALERYVGDESLKCGECKDEKIIPNGKKVAVVGSGPAGLANAASLRRMG
                     YDVDIYEALHEFGGVLQYGIPAFRLPKTIVESEVLRLEKLGIKFHKNVIIGKSLTIDQ
                     MKNELGYDAIFVGTGAGLPKSLGVPGEGHSGVFSANEFLIRVNLMKATPAMDYPTPIR
                     IGDNVAVIGGGNVAMDAARVAKRLGAKNVFILYRRDKSSLPARLEEVIHAEEEGIIFK
                     LLHNPVEFIGENHQLKAVRCEKMELGELDSSGRAKPVGTGQYETFPVDSAIVSIGENP
                     NPIISKATPGLETDRKGQIVVNNHQTSIAGVFAGGDVVLGAATVILAIGEGKDAAIAI
                     DEYLKKI"
     gene            complement(8857..9615)
                     /locus_tag="PLP02_05030"
     CDS             complement(8857..9615)
                     /locus_tag="PLP02_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPF42839.1"
                     /translation="MKKIVMAFGLIIVLSLAGCQQKGDMEVLTVLTSSGYEPYEMVDE
                     DGQLTGFDIELMEALASEAGIEIEWLDVSFEGIIASLQAGQYEVAIAGITPTPDRANV
                     VDFGEVYYNSENGITNYMVYEYENSYNTLDDLQGLVVGAQLGTIQAELLGELAEDYGF
                     TVELRDTNSQIIEEIKIGTINVLLVESLVADSIVEANSEFTKTELITDLDQLYGNAIA
                     FAKGSGYVDIFNNALEVLKDNGTLDYLIAKWFQE"
     gene            complement(9629..10345)
                     /locus_tag="PLP02_05035"
     CDS             complement(9629..10345)
                     /locus_tag="PLP02_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPF42840.1"
                     /translation="MITLIDVHKSFGDIEVLKGISHEFQKGKTTVILGASGSGKSTLL
                     RTINQLTPIDEGKVLFEGVDVTTIKHRQLVARIGIVFQQFNLFSGMNVLQNVMYTLVK
                     EKKLNKDKAREMAMISLDKVNMASRLDAYPKHLSGGEKQRVALARAMVLSPDVMLFDE
                     PTSALDPEKVNEVLDEIKKLTHIGLTNIIVTHEMGFAREVADEVIFMDEGRIIETGSK
                     EAFFQHPKAERTKQFLDKIL"
     gene            complement(10342..11028)
                     /locus_tag="PLP02_05040"
     CDS             complement(10342..11028)
                     /locus_tag="PLP02_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPF42841.1"
                     /translation="MDIFDYEKIKPWIPYLAKGIGITLLIAFVAAIVGFILGVVLVLM
                     NKRKFFRGFARGYIDIFRGTPMLLQLSIIYFAIPQLLDRIFNDGFGMNVDFAITALMA
                     AFITFGLNSAAYVSEIIRSGINSVDPGEIEAAKALGISKYHMYKDIVLPKAFKNSFPA
                     LVNEFVTLIKETSIVSVIGIQDLFRRQQIVTAQTYLYFEALIIIGVFYYVLIKVFSLI
                     ARRLEVIYAK"
     misc_binding    complement(11067..11285)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2977 a         2776 c         2554 g         3058 t
ORIGIN      
        1 cataatatga ttcgtatgca ttttcttctt tgtataaaga aaaaccaaaa gtctcgtcaa
       61 accaatcgcc aacatcatcc cagaagcttc tccaggagta ccctgtttca tcagtaaaca
      121 taataggatt attagcacaa taagcataca tgttggcaga taaaatatcg ccgactgctc
      181 ctaccattcc gtcagcactt ataaatctgc cgatcgtcgg atcataatat cttgactgta
      241 agtaatacca gcctgtttca taatcgtagt aataacttcg gtaacggaat ggatttacat
      301 tggcaatgtt taaattgtaa ctgtttatat agattgtttt tccccaagca tcatatttat
      361 actctgccac catattcccg tatgcatcag tcagaccgat gacatcaccc tgcaaattga
      421 tgatacagac ataaaagttg acttttccat tggtatcttt ttataaatat attgtataga
      481 atatttatta taaaaataac acgataaaat gcatttgtaa attgatatat aaaaaaataa
      541 gttatatttt tatattacct tttcataaca tatgttttgc agaaatgatt tgttaaaaaa
      601 agatcttcct taagcatcct ggtaaacagc tcgattaccg tttacttaat gaagatcaaa
      661 tatggataaa tagttctatt tactgactga aaccattttt tgtcaacggc ttttttcaga
      721 tgtcgtaata ccgaatgaat tcggccggat ggggaatctc gctgatttcc ctagcttccc
      781 gtcttttgcg ttcgcaccag actttgatga gttcttccgg gaacacgccg ctcttcgtca
      841 gataatcatg atccgactcc aacgcgtcga ttgcttcgat aagggaagtc ggtatcttgt
      901 cgattttacc tctttcatct tcggaaaggg aataaaggtt gaaatcgaac ggcccccaat
      961 ttccggcagc cggatcgatc tttttctcga tgccgtcgag tccggccatc agaatcgccg
     1021 aataggcgta atagggattg gctgtggcgt cgggattcct tagttcgaaa cgtttgacgg
     1081 atggtgtttt ggcataggcc ggcacccgga tgattgcgct acggttggca gtggcgaaac
     1141 cgacgacaac cggagcttcg aaaccgggag tcaaacgtct gtaggaattg gtcgacggat
     1201 tcgtaaaagc cgaaagcgaa gatatgtgcg acaaaatccc gcccatgaaa tacattgcga
     1261 tctgagacaa ctgggcataa ccgtccttgt cgtagaaaag cggttctccg tctttcttga
     1321 gaagcatgtg gacgtgcatg ccgtttcctg cttcgccacg gatcggtttg ggcatgaaag
     1381 tggcggtttt tcccgcccgg agggcgctgt tcttgacaac atatttgatc atcatggtgt
     1441 catcggccat cttttccatg ttaccgagtt cgacctctat ttcgtgctgg cccgaacctc
     1501 cgacctcatg atgatggtat ttgaccgcga tatctagctt ctcgagttcc agggacatgc
     1561 ggcttctgag atcgtaatcg gcatcctggg gaatgtccgc atgatatccg gcatcggcgg
     1621 ccagcatgta gccgtcgccg ctttgcctgg cgctatggct ccaggaggcg cggttacttt
     1681 cgagatggta atagacatct cccggcatca gtttgtattc ggctttgtca aagatatgga
     1741 attcgaattc cggtccgatg atcatttcat cggcaacccc ggatttacgc atgaattcga
     1801 tcgcgcgtct gctgacgttg cggggatatt gtccgaacgg ttcattcgtt tctccgatca
     1861 cataaacatc gccgatcatc gccaatgttt tggcttgggc aaacggatcg atggtcgcgc
     1921 ttgtcggatc gggaataaag atcatatcgc ttttttcaat cggagcgtat ccgtaattcg
     1981 aggcgtcgaa gccgattccg taacgaaata tgttttcctg aaagcggctg gcggggatcg
     2041 taagatgccg ccaccttcca tcaagatcga tcattttgaa atcgaccatt tcaatgttgt
     2101 tttccttgat gaattccttg attccgacca tgtttttcgt cattgctacc tccgaaagta
     2161 aacgcttgca tttaagatta taccagtatt atgggaaata tgcaacgaaa tcagaagtgt
     2221 ggtaaataaa agtcattttc atattgtcaa aggcaatgaa aaaggcgttt ttcagcgcct
     2281 tttatcattc atgtctcgtt acttggaacc tttccatgga gtaattctcg ccacttccga
     2341 tcccggcttt tctcaaaacg atattcactt gatcctcgac tgtgtcgaca ttgtcaagat
     2401 tcggtaaaag caaacccgac cggtatcctg acctgacgat gacaccgtat tttttgggat
     2461 ctagttccgc ctttgaggcg atcctctccg gcagttccaa cacatccacg ctatagatga
     2521 tatccttcag ttcggtggct tccactttgt caaaacgggg atcgttggtt ccggccgata
     2581 ccgcattttt gatgatctct tcggccacgg atttgctttg aggcatgatc gtaccgatac
     2641 aacctctcag tcttccgtgc tcatgaagcg aaacaaagac gcctgcttgt tttcttacta
     2701 gttcgtcggg aagaccttcc ggcaaaatca ggcgtttacc ggtcaaaaca taattttcca
     2761 aagacattct ggcaagacgg atatagatat cctctctttc tcttgttttg gatatttcct
     2821 tggtttcagc ctcacgggcc tgtttgtcaa aatcacgggt ggaatcggca cctgccggtt
     2881 caaacgaggc gatcccgtaa ccgacaccga atgggccttc ataagaaagc aattttggtg
     2941 tgaccttttc cttgtcgaaa agtcccgcca tgataacaaa agagcgcagt ccgcattcgg
     3001 cggccgactc cagaaaactt tccgaaaaag tcaaaaactt cagaaaatct cctttggcga
     3061 tcgccagagt gacatcctgg tcgaaaatgg gcccgtcagg actgaagccg taaggcccgt
     3121 cggcggacag tttatgcgat agatcaccgc ttgcgatgta gacgaccctc ctgttagttt
     3181 cttcgatcgc ttttctcaca catttgccaa attcgtaatg atcgtataat gacagaccag
     3241 atatcgacat ccggaccgat ttgtaatcgg catacttctg ttctatgaaa tagagaggaa
     3301 ccatcgttcc gtgatcgagc gacttgtcgc gttctcccag cgtgcccgct ctcagattgc
     3361 actccttcgc ctccctgacg atatgttcga ccagttcgtc atcgtattct ttcgccatac
     3421 cgacgctttt gactccgaaa aggcggaaat cgccggtagc gaaatttccc ggtgaaatat
     3481 ggatgtaatc ggaatacatg atggagtgcg gggaggaaac gatgatcgtt tcgggggcta
     3541 actgggcgat ttcctccgcc accttttcaa aagcggcgat tgtcttgccg atcttttcga
     3601 tctcgtttcc accgatcgcc gggacggcaa gcggcggatg cggcatgata tatgctttca
     3661 atagcggcat attagttcat ctcccattcg cggatatttc caatgtggat attttcaaga
     3721 ccggcgtttt cgccgatcgc ccgggctctt ttcaaggttg tggccggcgt aggttcgcct
     3781 tcgtccatcc gccatgccgg gaaaaaacgg ctgatgtgcc agggagtgtc cttacccagt
     3841 ttttcggcga tgaaattcgc cattttggtc agttcactat cgctgtcgtt cactccggga
     3901 acgacaaggg tcgtcacctc taaatggatt ccggcttttt tcatggtttt cacgttggaa
     3961 agaacgcttt cgacatcgcc gccgcaataa gcgcggaaaa cgtcgtctct tatcgacatc
     4021 aaacggcgtt ttgatcgaca gttcgttcgc cgaatcccac ggctacaaca tcggagacac
     4081 tatccaaatc gaactgacgg catattcgct ttctttcgaa acacaaatca ccgggacaat
     4141 gtgctatgcg gaagccactt cgacgaaatc agtttgtccc atttatataa gcatcgcaca
     4201 aaaccagggt ttgttgccgt ttttcaatgc atacgcaaac caagcgcttg tcaaaaccgg
     4261 cgatgttgca tatacaaggc aagcgatcaa caaccattat agcgaccaaa acaacttgct
     4321 tttcattctg gatatggaca ctaccgagtc gttcactttg cttgaaaacg aggtttccca
     4381 aagcaaggcc atgatctatg tgttcccggt aatctttctt tttgtttctg tccttgtgat
     4441 cttgacgacg atcagccagc tgatcctgag agagagaatg aatatcggaa cattgaaagc
     4501 tctcggttac agcaatccag aaatcgcctc tcattattcc tcagtgggaa tcatcgtcac
     4561 gctcttcggg gcgttgattg gagtaatgat cggaccgaaa atcattccgc atgtcatgga
     4621 agtcaaatat gaactcgtat acaatcttcc ggcttatgac ggcgtaatat acagtatcct
     4681 ttggagtttt gcggccattt tggccgtcgg attattgtcc gcttccttaa gctttttgat
     4741 ctgtcgggac gttctaaagg agactccggc ccagtgcatg cgcccgaaag cgccgaaaaa
     4801 catgatgatg atcagattga tcggaggtga cggacatgat gaaccagtcg cgcctgtcga
     4861 ttaagatggc cattcgcaat atcatcgtcc atcccggaag agcgttcatg agcgtcattg
     4921 gcgtgatggg ctgcgtagca ctactcgtct gtgccttcgg aatcggcgat tcggttaatt
     4981 acagtatcga caacgaacta aaccgccaat tcacatacga catcaatacg acatacgaca
     5041 aagcaaatga aaccgtccta tatgattatc ttgataacaa aggtgccgat tacgaaccat
     5101 accgctcctt cctggcaacc gtttccggat atagcgaagc ctctatttac gtttatgtcc
     5161 tggaaagtcc ttccgctcac accactattg atgtttcgga aggcgtcgcc atcagccgtt
     5221 ccaaagccga tacaatcggc gtaaaggaag gcgatgaaat cacgatttcg gtcgcatccg
     5281 gcagtgtcgt tgtcaccatt gattcattgg ttgacacttc tgtcactcag ggggttttta
     5341 tttccgaagc tgatttcggg ctcattgatc tcgatgccat tctcacatac catctctgga
     5401 tttccctcta cggagcaaca gatgaaatag ttgatgagat caactcaatc aacggaacca
     5461 acggcgcttg gctcaaaagc gaccgccagg cgatgatcga tgacaaagtg acttcgatca
     5521 acactatccg caaaaccatg atgattttct cgattttgct ttccgtcgtc gttctttaca
     5581 acttctcgct tttgaacatg aaagaaagca tccgcgacat cgccaccttg aaagttctcg
     5641 gccacaaaaa catccggatc ggcaaaatct tggtttatca gatgatgatc ctggtgtcaa
     5701 tcggaactat tttcggattg ttgcttggtt atcccatgct ctatcttgtc atgagtagca
     5761 acaaagtcga tgtgatggcc tatctgtatc atatcgaacc ggcatcctat gtctatgcgg
     5821 ccttgctgtc gatcggaacg ggattcatat tcaacattgt gttcacaacc tatatccgca
     5881 agattaaaat ggtcgaaagc cttaaaagca tcgaataatc ccataatacg gaggtattct
     5941 agatgatttg gcgccatata aaggtagtcg ttttatttac attcgtcgtc atgttgggaa
     6001 tcctggcatt cattccgctt cccgattttc cagcgaccgg attctacgaa acattcgaaa
     6061 cgatatacgg cgatgaaatt gccgacaaaa atctaaagta tttggccttt gacgagactt
     6121 cccttccgat cggataccgt ccctatttca acacgctcat gagacaatat tgccgtagaa
     6181 accatatgag atacctgatt gccacatatg atgaactgaa agaagacgga tatatcatcg
     6241 atggcggatt ccaaaacggc gtcattcttc actttcacaa catatcgatg acaggcaatg
     6301 acaacatcag tggagaaata tccatataca gcggctccct ttccgcaaca gggagggact
     6361 tctttactca atatgacgac ggctggattg tcaacacttc gaattctttt tggataagct
     6421 gatcgggtta atcaatacct taaattgtcc ttatttgtca aagaacgaaa agacggtttg
     6481 ctaaaaccgt ttttttgtgt cagaaaaagg attttttata ctcataaaat ccttttggac
     6541 caaatttgtc gcgataagca tgacgataag tgatataatt attttgaaga ttagaaaaga
     6601 aagggacatc atgccatgta cgaaataatc gaaaagaaac cacaaaacgc cgatgtcgat
     6661 cttttggaaa ttctggcgcc gatggtcgcc aaaaacgcaa agcctggtca gtttctgatc
     6721 atcagggtag acaaaaacgg ggaaaggatc ccgatgacga tcgtgtcgca tacagaaaca
     6781 accgtcacga tcattgttca gaaggtgggc tattccacca tgcttcttgg caaaatgcaa
     6841 aaaggggacg ctttggaaga tgtcgtcgga ccattgggaa tgccaacaat catcagaccg
     6901 gttaaaaaag ttctcggcat cgccggcggc gtcggagccg ctccgcttta tccgcagctt
     6961 agggaatacg cttccagagg atcatccgtc gaccttataa tcggagccag aaacagggat
     7021 catctgatcc tgaaggatga cttcgcggct ttctgcgaga atatccatat tgcaacagac
     7081 gatggcagta tcggaaccaa gggattcgtc accgacgtcg ccaaacgcct ctatgaaacc
     7141 gaaacctacg atcttgtcat cgccatcgga ccgcttgcga tgatgagagc ggttgtcgaa
     7201 ctagataaaa aacatggtct caaaaccgat gtatcgctga atccgatcat gatcgacgga
     7261 acaggaatgt gcggaaactg ccgggtgtcg attgccggaa aaacttattt tgcctgtgtc
     7321 gacggaccgg attttccggg agatgacatc gattttgacg aacttctggc cagacaaaac
     7381 tcctaccgcg aggaggagca tatctgccgt atcaaacttg gtgataaaaa atgatcaaag
     7441 acagaattat tatgcaaacc caagatccct tgatccgtaa ccataatttc aatgaagtcg
     7501 ctttaggttt ctctgagaat gaagccattc ttgaagcttc gagatgcctg caatgcaaga
     7561 atccgacttg tatcgccgct tgtcccgcta gtgtcaaaat tccagatttc atccgcctga
     7621 tcaaggaaaa aaacagccgg gtcgcttatg acacgatata caaggacaat gtcctgccgg
     7681 cggtctgcgg cagagtttgc ccgcaggaaa gccagtgtga aggcgcctgc gtcttgaatc
     7741 gtacgggccg tccgatcgcg atcggcgccc ttgagcgtta tgtcggggac gaaagtctca
     7801 aatgcggcga atgtaaagat gaaaaaatca ttcccaacgg caaaaaagtc gccgttgtcg
     7861 gctccggtcc cgccggattg gcaaatgccg cttccctgcg tcggatggga tacgacgtcg
     7921 atatttacga agctttacat gaattcggcg gcgttctcca atatggcatt ccggctttca
     7981 gattacctaa aacaatcgtc gaaagcgaag tattgcgcct tgaaaaactg ggaatcaaat
     8041 ttcataagaa tgtcatcatc ggtaaatcgc tcaccatcga ccagatgaaa aacgagctcg
     8101 gatacgatgc catattcgta ggcaccggcg ccggacttcc caaatcgctt ggagttcccg
     8161 gcgaaggaca tagcggcgta ttttccgcca acgagttcct gatccgcgtg aatctgatga
     8221 aagcaactcc tgccatggat tatccgacac caatccgaat tggcgataac gtggccgtca
     8281 tcggtggcgg caatgtcgcc atggatgccg ccagggttgc caaacgcctg ggagcgaaaa
     8341 atgtcttcat tctttaccgg cgtgacaaat cttcgcttcc cgcccggctg gaagaagtca
     8401 tccatgccga agaggaaggc atcatattca agttacttca caatcccgtc gaattcatcg
     8461 gcgaaaatca tcaattaaag gctgtcagat gcgaaaagat ggaactaggc gaattggatt
     8521 cgtccggcag ggcgaaaccg gtcggcaccg gtcaatacga gactttcccg gtcgattccg
     8581 ccatcgtttc gatcggggag aatcccaatc ccatcatcag caaggcaact cccggtcttg
     8641 aaacggacag gaaaggccaa atcgtcgtaa ataatcacca gacatcgata gcaggtgttt
     8701 tcgccggcgg cgacgtcgtg ctcggagccg ccacggtcat tctggcgatc ggagaaggca
     8761 aggacgccgc gatagcgatc gacgaatatc ttaaaaaaat ttgaaataga ataattaaca
     8821 atcaaaagcc cgaagacatg cgccttcggg cttcttttat tcctggaacc actttgcaat
     8881 caagtaatca agcgttccgt tatctttcaa gacttccaga gcgttattga agatatcgac
     8941 atagccgctt cctttggcaa aagcgatcgc atttccatag agctggtcga gatcggttat
     9001 aagttctgtc ttggtaaatt cgctgtttgc ctcaacaatc gagtccgcca ccagactttc
     9061 aaccaaaagc acgttgatag taccgatttt gatctcttcg atgatctggg aattggtgtc
     9121 tctcagttcg accgtaaatc cataatcttc cgccaattcg cctaaaagtt ccgcttggat
     9181 ggttccgagt tgcgctccta ccacgagtcc ttgaagatcg tcaagcgtat tgtaactatt
     9241 ctcgtattca taaaccatgt aattggtaat gccattctcg ctgttgtaat agacttcgcc
     9301 gaagtcgaca acatttgcac ggtcaggtgt cggggttatg ccggcgatcg ccacttcgta
     9361 ctggccagcc tgcagactgg cgatgatacc ttcgaagctg acatcgagcc attcgatctc
     9421 tattccggct tcgctcgcca aagcttccat cagttcgatg tcgaatccgg tcaattgacc
     9481 gtcttcatcc accatttcgt atggttcgta accgctcgag gtcagaaccg tcaatacttc
     9541 catatcgcct ttttgttggc atcccgccag cgacaatacg ataatcaatc caaatgccat
     9601 gacaattttc ttcataattt tatcctcctc acagtatttt atccaaaaat tgttttgttc
     9661 tttctgcttt cggatgttgg aagaatgctt ccttggaacc ggtctcgata attctccctt
     9721 catccatgaa aatcacttcg tccgccactt ctctggcgaa tcccatctca tgggtcacga
     9781 taatgttcgt aagcccgata tgggtcagtt tcttgatctc gtccaacacc tcgttgacct
     9841 tctccggatc cagtgccgat gtcggctcgt caaacagcat gacgtcggga gaaaggacca
     9901 tcgctctggc cagagccacc ctttgcttct cgccgccgga aagatgtttg ggataggcat
     9961 ccagcctgct tgccatgttg accttgtcaa gcgaaatcat cgccatttct ctggctttat
    10021 ccttgttaag tttcttttcc ttaaccaggg tatacatcac gttttgtaag acgttcatgc
    10081 cggaaaacag gttaaactgc tggaagacta taccgatcct ggctaccaac tgccggtgct
    10141 tgatcgtggt cacgtcaacg ccttcgaaaa gtactttccc ttcatcgatc ggcgtcagtt
    10201 ggttgatcgt ccgcagcaag gtcgatttcc cgctgccgga agctcccaga atgaccgtgg
    10261 tctttccttt ttggaattca tgactgatgc ctttcagcac ctcgatgtcg ccaaaagact
    10321 tatgtacatc aatcaatgtt atcattttgc gtaaatcacc tccagacggc gggctatcag
    10381 gctgaatact ttgatcagga cataatagaa tacaccgatg atgatcaaag cctcgaaata
    10441 cagataggtc tgggcagtga caatctgttg ccggcggaaa agatcctgga tgccgatcac
    10501 gctcacgatc gatgtttctt tgattagagt gacgaattca ttcacgagag ccggaaacga
    10561 attcttgaag gctttgggta aaacgatgtc cttatacatg tggtatttgc taatcccgag
    10621 cgctttcgcc gcttcgatct cgcccgggtc gacgctgttg ataccgctac ggatgatttc
    10681 cgacacatag gcggcactat tcagaccaaa tgtgatgaac gccgccatca gggcagtgat
    10741 ggcgaaatcg acgttcatgc cgaatccgtc gttgaagatt ctatctagca gttggggaat
    10801 ggcaaaatag ataatcgaca gttggagcag catcggggtt ccccggaaga tatcgatata
    10861 accgcgggcg aatccgcgaa agaattttcg tttgttcatg aggaccaaaa caacgcccag
    10921 aataaaaccg acgatcgccg ccacaaaggc gatcaaaagc gtaatgccga tacctttggc
    10981 gagatagggt atccacggtt tgattttttc ataatcgaat atatccatga aatcactcct
    11041 tgaaataaaa aaagaccgtc cttaaaaaca aggacgatcg aatgaacgcg gtgccacctt
    11101 tgattcccgg ccatgatgca tgccggactc tcatacccat aacgcaggtt ctgcgtgacc
    11161 gcctcaacaa gtttcgacga tccgactccc agtcacgttc gttttccttc cctcatcgcc
    11221 tttcaccaac cggcgactct ctatagacca tcgggaaaac tactactact gttcacggtt
    11281 ttccacgatt atgccacaga gaaaaagatt tgacaagggc ttttaaaatg aaaatgtttt
    11341 cagtggcgaa aataaatatc gtaac
//
LOCUS       DAOPJL010000036         9338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_7768_length_9338_cov_5.144996, whole genome shotgun sequence.
ACCESSION   DAOPJL010000036 DAOPJL010000000
VERSION     DAOPJL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7768_length_9338_cov_5.144996"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLP02_05045"
     CDS             complement(<1..912)
                     /locus_tag="PLP02_05045"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPF42842.1"
                     /translation="MKKLLRYGFTLAFTFAMVLILAGCTRNITVSFQTNGGDVMANAT
                     LEQGATLDNVSDPTRTGYSFAGWYYDAALAEPIDITEPVLRNITIYASWSVNDYTVTF
                     VVEDETVQTSDITYGNALPFPADPVKTGYDFDGWYVGEDEVDSSTRISSDTTVVAHFT
                     LAYYTITFHLGNDTTDEINDAIYGEAPDNIPQSALTPQAGKDIVWMYEVSADVYAEVD
                     FTEISADIDVYSMYVDQYFTVTFMNGTTVISTASIKYGNVVARPSTDPVKEHYNFVSW
                     IDSDSLVYDFSTPITEDTVIYANFSIQK"
     gene            complement(927..1376)
                     /locus_tag="PLP02_05050"
     CDS             complement(927..1376)
                     /locus_tag="PLP02_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42843.1"
                     /translation="MNKKLLIFLVILLGALSVMAIGIWGSLPDPSDSVMVENLEFSDS
                     RIVWNEDETEKIIFVGDIITDLEENYDMTIAFTYAPDDANTDYLVAYADDSSVGAVLQ
                     DHQVYVTFSEKTSVTITIKDKKTGETDTVTLIFQAPGSVDVPDDIFD"
     gene            complement(1391..3004)
                     /locus_tag="PLP02_05055"
     CDS             complement(1391..3004)
                     /locus_tag="PLP02_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42844.1"
                     /translation="MWNRLLILTLMQLSNKMNLRFIHNRRFAGKVAVGFVLLVVLSVV
                     MAGLLYLVKNILYIPVNVYFVIFFLIITQVISIISATNGLIIDLYSSKENQILLAFPV
                     KNDEVFVSKLMVYFFHELIKNFYFLIPLLIGFGYVNSMPWWYYLNILPVSIMLPMVAV
                     LTSALLSIPIMYIKHFFSAKNAFSLIAAILMIGGLFYFVTVLVANIPIPIRIVQLYNR
                     FVSNLAIFMQSSAKYGTIYTMIGKLLFNMNMLINYGLVLGIIGGMLLLVFLISRPLYF
                     RLASKTLEFAVDKQHKSHEEKTSKSIFLTFLRKEWLVTKRSVNELLGNYALLFLFPFV
                     LYILNYIYLGIPRSSLGTALMVAFDLLISLFLVSASNTASASAITSEGYEFILLKTAP
                     SKTNRIVWAKITINVVFSSLIIIISFLMFRFALPNFPVSDILPMMVTILILNTTHILW
                     SLQIDVLNPHLAEYASSGSLSGNPNVQKSVTMGFGMSLVFGVIFIALCFVLPDKAWLI
                     IISLSVVFFAYRLFSFINFLGAYFGDIEF"
     gene            complement(2994..5207)
                     /locus_tag="PLP02_05060"
     CDS             complement(2994..5207)
                     /locus_tag="PLP02_05060"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPF42845.1"
                     /translation="MTYYVLLAGIVLGIGINLLIIRKYPAKIEKYFKLLAVLLFAVYS
                     VRLFSEDVFNDTFNLLYVDVTTPIDADSTWVLPRNISLILVFLRWATLMAIVWGLLSP
                     FFDQRFLKFFMATFGLTIGVLNIVFFRENIIAFIGTDNFLSYRSIQFIAEIIIFMTLV
                     VMNIFMIIREKLVPKWRDVLNLFLISGIASLAVMPLTSLYNLLGEYGAITDDFNKEHI
                     IFLVLPFVIMLVSYFVMFNKRQKDRDLMLLYLVFAAYIDYFFERRTGLGGLPLHLCNT
                     AIILMLFSFVFRLKGVFYFNYFANVLGALVAIVLPSIGTEDMYSMVSIHYWYNHWYAF
                     FIPILGVAFHTFERPTLKLMVKAIGVFTAYYIVVLFLNAWFNNYTWTDYFFTYGDNIS
                     GRFNVERLQYENVVSFTWNNLTFTFFWLYQILLYLSFIFLMFASWYVYDAIFQFFDAY
                     YALIIKKRKMRMDMLNLRKLLNGKDIHDPIDPGGINMLKISHFTKQYGNSKRKAVDDF
                     SLEVNDGEVFGFVGHNGAGKSTTIKSLVGIQSITEGEMEICGYSIKTQPLEAKRNIGY
                     VSDNHAVYEKLTGREYIHYVADLYKVPADLREERMANYLDKFNLAFAVDNEIKSYSHG
                     MKQKLVVIASLIHDPKVWILDEPLTGLDPTSSIQIKETMREHANRGNIVFFSSHVIEV
                     VERICDRIAIIAHGKLVGVYVVKDFVSKGMSLEKLYLESMAKKPAAIPEVKVNVE"
     gene            complement(5383..6108)
                     /locus_tag="PLP02_05065"
     CDS             complement(5383..6108)
                     /locus_tag="PLP02_05065"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HPF42846.1"
                     /translation="MKTLLLYLLIHIVASSFQLIWQGNKRKNIMSVVYPSVIYFVLIF
                     LPYLVMGFQLSMLITGIALGLSHFIIVLMGFYLECQPGMDENKKNRLYLDLFCQLLSI
                     FIVVLVVALFRSGYVYDETPASTFLLITNYGWIRVIASILLAWIPGSLIIKSVMKERK
                     IPADVSQKNSGEKIGVLERALLLVTIYFGMYTAAVLILVVKGIMGLGITKDSPEESER
                     MIMGTFVSLLYTIVVAAVCFFLL"
     gene            complement(6105..6782)
                     /locus_tag="PLP02_05070"
     CDS             complement(6105..6782)
                     /locus_tag="PLP02_05070"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HPF42847.1"
                     /translation="MRKYIALMGEMSPPVDAKEKDTLQEKLLQEFKIMNVSMENDLAS
                     IFILTAKNMFLALWNGAERLMEAIEKIRFTVKPYQVSFGIGIGDITTGIDKTHNLRSD
                     GPAWWEAKSALDFAKKSKRKTYGVTDAVISGFSDCDLSSLVGDSMVLMHAVRQRWTQG
                     QEELVEEIVKYRGFSTDLVQKDLAINLGMDQPSLNKKMKSTLICDYFRTANTIQSLID
                     KELKDNK"
     gene            complement(6990..7865)
                     /locus_tag="PLP02_05075"
     CDS             complement(6990..7865)
                     /locus_tag="PLP02_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPF42848.1"
                     /translation="MADFVLFTDSCSDLSLAMVKKIGVEVIPMSFMVDDKIYRNYPDE
                     REMTTKNFYDLIRNKKTSSTSQLNPQEFDDIFRKYLEKGLDVLYIAFSSALSGTYASA
                     VQAGPNLEKEYPGRKVVAVDSLAASMGLGLLTWHAATLKKQGKTLEEIQEWLEKNKKR
                     LDHLFTVDDLMYLKRGGRLSAVVATIGTIIKLKPLLHVSPEGKLVQTGVARGRRQALN
                     RMVDRMMQTIEQPLELPIFISHGDCPEDVEYMSEQVKMRIPGAIIETGYIGPVIGSHS
                     GPGTIAIYYLGNDRL"
     gene            complement(7883..8458)
                     /locus_tag="PLP02_05080"
     CDS             complement(7883..8458)
                     /locus_tag="PLP02_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42849.1"
                     /translation="MKKKLAKVIRIISTAPIVALIFVSTLYLFVPESFQTVWEYLFSV
                     TVLSLLPLLAYPTQWIFKIDKEDTRAGERKTAIVFSVIGYVVGAVITFVFNFTDIQKI
                     FYLTYLLSGATIAFSSFVLKIKASGHMCGVAGPIAAMVYFGGPGYLGLTIFLGFVIWS
                     SLYLGRHSPRELVYGTLIPVGALVLALLIVL"
     gene            8637..9236
                     /locus_tag="PLP02_05085"
     CDS             8637..9236
                     /locus_tag="PLP02_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42850.1"
                     /translation="MINENTLRETIAKNLTRYRKANNLTQLQLAEKLNYSDKSISKWE
                     RGEGIPDLLVLASIADLYDITINDIVTDRKKLAHQIHAFNKIMISLISFTGVWFLATI
                     IFVTLRLFVPELTDRAWMSFIYSIPISLIVLLVFASIWGKRIHLFFCVSLLSWSVPLA
                     LFLSFDYLNLDLWLLFIAIIPLQVITIMAFILKSKKTKS"
BASE COUNT         2791 a         2058 c         1887 g         2602 t
ORIGIN      
        1 tttctggatc gagaaattgg catagatgac tgtgtcttcc gtaataggcg tcgagaagtc
       61 ataaaccaag gaatccgaat cgatccagct gacaaagttg taatgttcct tgacaggatc
      121 ggtagacggt ctggcgacaa cattgccata tttgatggaa gccgtcgata tgacggttgt
      181 tccgttcatg aacgtgacgg tgaagtattg atcgacatac atcgaataaa catcgatatc
      241 ggcggatatt tccgtgaaat cgacttcggc gtaaacatca gcggaaactt catacatcca
      301 aacgatgtcc ttgcccgcct ggggagtcag ggcggactga gggatattat ccggagcttc
      361 tccgtaaatc gcatcgttta tctcatcggt cgtatcgttt ccgagatgga aagtgatggt
      421 gtagtatgcc aaagtgaaat gcgcaaccac tgtggtatcg gaagatatcc gcgtagagga
      481 atcaacctcg tcttcgccga cgtaccaacc atcgaaatca tatccggttt ttaccggatc
      541 ggccgggaac ggcaaagcat tcccgtatgt gatatcggat gtctggactg tttcatcctc
      601 gacaacaaaa gtcaccgtat aatcatttac gctccaactg gcgtaaatag taatattacg
      661 gagcacgggc tccgttatat cgataggttc tgcgagtgcc gcatcataat accatccggc
      721 aaacgaatac cctgtccggg tcggatcgga aacgttatcg agcgtagccc cttgttctaa
      781 ggtggcattt gccattacgt ctcctccatt tgtctggaac gatactgtaa tgttgcgcgt
      841 acatcccgcc agtatcagaa ccattgcgaa tgtaaatgct aatgtaaatc catatcgcaa
      901 taacttcttc attttatcct ccttatttaa tcgaaaatat catcgggcac gtcaacagaa
      961 cccggagctt gaaatatcaa agtgacagta tccgtttcgc ctgttttctt gtccttgatc
     1021 gttatcgtga ccgatgtctt ttcagaaaag gtcacatata cttgatggtc ttgtaaaacg
     1081 gcaccgacgg atgagtcgtc cgcgtacgca actagatagt cagtattagc atcgtccggc
     1141 gcatatgtaa aagcgatggt catatcataa ttttcttcca gatcggtgat gatatcaccg
     1201 acaaaaataa tcttttcggt ttcgtcctcg ttccagacaa tccgactgtc agaaaattcg
     1261 agattttcta ccatcactga atcggaggga tccggtaaag atccccaaat accgatagcc
     1321 atgacagaca gcgctcctaa aagtattact aggaaaatga gcaacttttt gttcacggtc
     1381 tttcacctct ttaaaattcg atgtcgccga agtaggcgcc aaggaaattg ataaacgaga
     1441 atagtctgta ggcaaaaaac acaactgaca gactgataat gattaaccag gctttgtccg
     1501 gaagcacgaa acacagggca atgaaaatga ctccgaatac caaactcatc ccgaatccca
     1561 ttgttacgga tttctggacg ttcggattgc cggacaaaga accggatgaa gcatattccg
     1621 ccaaatgcgg attcagcaca tcgatctgca gactccagag aatgtgcgtt gtattcaaga
     1681 taaggatggt caccatcatc ggtaagatgt cggagacagg aaaattggga agcgcgaaac
     1741 ggaacatgag aaaactgatg attatgataa gcgatgaaaa gacaacattg atcgttatct
     1801 tggcccacac gatacggttg gtcttcgaag gcgcggtttt cagaagtatg aactcgtatc
     1861 cttcactcgt gatcgccgaa gccgaagctg tattcgaagc gctgaccaag aataaactga
     1921 taagcaaatc gaaggcaacc atgagtgctg tcccaagcga acttctcgga attcccaaat
     1981 aaatataatt aaggatataa agaacaaacg ggaacaggaa cagaagggca tagttaccca
     2041 atagttcgtt gacgcttctt ttcgtcacca gccattcctt tctcagaaat gtcaagaaaa
     2101 tgctcttcga ggtcttttcc tcatggctct tatgctgttt gtcgacggcg aactccaaag
     2161 tcttggaagc caaccgaaaa taaagcggcc gggaaatcag aaacaccaat aaaagcattc
     2221 ccccgatgat tcccagaacc aatccatagt tgataagcat gttcatgttg aaaagcaatt
     2281 taccgatcat tgtgtatatg gtgccatact tggccgatga ttgcatgaat atcgccaaat
     2341 tgcttacaaa acggttgtaa agctggacga tgcggatcgg aatcggaata tttgcgacaa
     2401 gcacagtgac aaaatagaaa agtccgccga tcataagaat cgccgcaatc agggaaaaag
     2461 cattcttcgc cgagaaaaaa tgcttgatat acataatggg aatcgataaa agcgctgatg
     2521 tcagaaccgc aaccatcgga agcatgatgg ataccggaag aatattcaga taataccacc
     2581 aaggcatcga attgacataa ccaaagccaa tcaacaaagg aatcagaaaa taaaagttct
     2641 taattagttc gtgaaagaaa taaaccatga gcttgctgac gaaaacttcg tcatttttga
     2701 cgggaaaagc aagcaaaatc tggttttcct tgctcgaata aaggtcaatg atgagcccgt
     2761 tcgttgcact gataatactg atgacctgtg taatgatcag aaagaaaatg acgaaataaa
     2821 cgttgacagg gatatacagg atgtttttta caagataaag caagcccgcc atcacaaccg
     2881 acagaacaac gagcaaaaca aaacctacgg caactttccc ggcgaacctg cgattgtgaa
     2941 tgaatctcag gttcatcttg ttggaaagtt gcatcaatgt caggataaga agcctattcc
     3001 acattgacct tcacctcggg aatggctgcg ggtttcttcg ccatgctttc aagatacaac
     3061 ttctcgaggc tcatgccttt agagacaaaa tctttgacaa catatacacc aaccagtttt
     3121 ccatgggcga tgatggcgat ccgatcgcaa attctttcga cgacttcgat gacgtgacta
     3181 gagaagaaaa ctatgtttcc cctgttggca tgctcgcgca tggtttcctt gatctggatc
     3241 gacgatgtcg gatcaagtcc ggtcaatggt tcgtcaagaa tccatacttt cgggtcgtgg
     3301 atcagagaag caatgacaac cagcttctgc ttcattccgt gggaatagct cttgatttca
     3361 ttatcgacag cgaaagccaa attgaatttg tccaaataat tcgccatgcg ttcttcgcgc
     3421 aaatcggcag gaactttgta cagatccgcc acgtaatgga tatattcgcg gccggtgagt
     3481 ttttcataaa cagcatggtt atcagaaaca taaccgatgt ttcttttcgc ttcgagtggc
     3541 tgcgtcttga tcgaataacc gcagatttcc atctcgcctt cggtgatcga ctggatcccg
     3601 accaaactct tgatcgtagt ggatttgccg gcgccgttat gtccgacgaa accaaagacc
     3661 tcgccgtcgt tgacttccaa ggagaaatcg tcaaccgctt tccttttgga attgccgtac
     3721 tgttttgtaa agtggctgat ctttagcata ttaatccctc ccggatcgat tggatcgtga
     3781 atatcttttc cgttcaacaa ttttcttaaa ttaagcatgt ccattcgcat ttttcgcttt
     3841 tttatgatta aagcataata cgcatcgaag aattggaaga tggcgtcata gacataccag
     3901 ctggcaaaca tcaggaagat gaaactcagg tagagcaaga tctgataaag ccagaagaac
     3961 gtgaatgtga gattgttcca cgtaaatgaa actacatttt catactgtaa tctctccacg
     4021 ttgaaccgcc cggaaatatt gtctccgtaa gtaaagaaat aatcagtcca agtatagttg
     4081 ttgaaccagg cattcaggaa gaggacaacg atgtaatatg cagtgaacac cccaatggct
     4141 ttgaccatca gtttaagcgt cggtctctca aatgtatgga atgccactcc caaaatagga
     4201 ataaagaaag cataccaatg gttgtaccag taatgtatgc tgaccattga atacatatcc
     4261 tcggtaccaa tacttggaag cacaatcgcg accaaagcgc ccaaaacatt tgcgaaatag
     4321 ttgaaataga aaacgccttt gagacggaac acaaaagaaa acagcatcaa aataatggcc
     4381 gtgttacata ggtgcaaggg taatccgccg agtccggtgc gtcgttcgaa gaaataatcg
     4441 atatatgctg caaaaaccaa atataatagc atcaaatcgc gatctttctg tcgcttattg
     4501 aacataacaa aatacgatac caacatgata acaaaaggca atacgaggaa aattatatgc
     4561 tccttgttga aatcatcggt gattgcaccg tactctccca aaagattata tagggacgta
     4621 agcggcataa ccgctagact tgcaatgccg gaaatgagaa acaggttcaa aacatcgcgc
     4681 catttcggta ccaatttttc ccgtataatc ataaagatat tcatgaccac gagagtcatg
     4741 aatataatta tttccgctat gaattggatg ctgcggtatg aaagaaaatt atctgttccg
     4801 ataaatgcaa tgatgttttc acgaaaaaat actatgttca agacgccgat cgtaaggccg
     4861 aaagtcgcca tgaaaaattt taagaatcgt tggtcaaaga acggggacag caatccccat
     4921 actatggcca taagtgtcgc ccaacgtaaa aaaaccaaaa ttagtgaaat gttacgcggc
     4981 agcacccatg ttgagtcggc atctatcgga gtcgtgacat caacgtaaag caaattgaag
     5041 gtatcgttga aaacgtcttc tgaaaaaagc ctcaccgaat aaacagcaaa caacaacacg
     5101 gctaacagct tgaagtattt ttcgattttt gcgggatact ttctgataat caggaggttg
     5161 attccgatac caagaacaat accggcaagt aatacataat aagtcatcga ttacactcca
     5221 tctgaaaatt tcataatttt ccggtgtcta gcatgccaag caataaccaa attattttgc
     5281 tatggtcata ctgtttttat attatatcat aaattgaaaa tttatatagt gttaaaaaca
     5341 gaaaaatgta agattggaac gcaaattttt ttcactatac ttttaaagca agaaaaagca
     5401 cactgcggca actacgatgg tatacagcaa ggaaacgaaa gtgcccatta tcattctttc
     5461 ggattcctcg gggctgtctt tcgttattcc cagtcccatt attcctttca ccaccaaaat
     5521 aagaacggcg gccgtataca taccgaaata aatggtgacc agtaacaacg cacgttcgag
     5581 gacgccgatt ttttcgccgc tgtttttttg gctgacatcc gcagggattt tcctttcttt
     5641 catcacggat tttatgatta aacttccagg aatccaggct aagagaatgg aagctatcac
     5701 tctgatccaa ccataattgg ttatcagaag gaaagtagac gctggagttt catcataaac
     5761 ataaccgctt ctgaacaggg caacaaccaa tacgacgatg aatatggaca ataattggca
     5821 aaatagatcc aggtatagtc tgttcttctt gttttcgtcc attccaggct ggcattccag
     5881 gtaaaaaccc attagaacaa tgatgaaatg agaaagtcca agggcgattc cagtaatcag
     5941 catcgacagt tggaaaccca tgacgagata gggcagaaaa atcagaacga aatatatgac
     6001 ggaaggatat accaccgaca taatgttctt tcttttattt ccctgccaaa tcaattggaa
     6061 actactggca acaatatgta tcagcaaata caaaagcaac gttttcattt gttatccttc
     6121 aactccttgt caattagaga ttggatggtg ttggcggtgc ggaaataatc acatatgagg
     6181 gttgatttca ttttcttgtt cagactgggt tggtccattc cgagattgat cgccagatct
     6241 ttttggacaa ggtcggtgga aaagcctctg tacttcacta tttcttcgac caattcttcc
     6301 tgcccttgcg tccatctttg tctcacggcg tgcatcaaaa ccattgaatc gccgactagc
     6361 gaggataaat cacaatcgga aaaaccagaa atgacggcat ctgtcactcc gtatgttttt
     6421 cgttttgatt ttttcgcaaa atccagcgct gatttggctt cccaccatgc cggcccgtcg
     6481 gaacgaagat tatgtgtttt atcgattccg gtcgtaatgt ctccgattcc gattccgaaa
     6541 ctaacttgat acggcttgac ggtaaacctg atcttttcaa tggcttccat gagccgttcg
     6601 gcgccattcc aaagagcaag aaacatgttt ttggctgtca atatgaaaat tgaagccaaa
     6661 tcgttttcca tagacacgtt cataatcttg aattcctgca atagtttttc ttgcaatgtg
     6721 tctttctctt tggcgtcaac aggcggactc atttccccca taagagcaat atatttcctc
     6781 atgctaacac ctcacaagcg atattttacc tttattttcc tcttatttca atatgattgt
     6841 cattatttat tcaatattgc tataatttcc attatttagt ttttatatgt tataattcct
     6901 tttttagtgc aatattataa tattttccgt tttatatgca ataaaaagcg ctattttttt
     6961 gaaatagcgc atttgtcgac ttgtttatat tataaacgat cgtttccgag atagtatatg
     7021 gcgatggttc ccggtccgga atggcttccg atcactggac cgatgtatcc ggtttcgatg
     7081 attgctccag gtattctcat ttttacctgc tcactcatat attccacgtc ttccgggcaa
     7141 tctccgtgag aaataaatat cggaagttca agcggttgct cgattgtttg catcatccga
     7201 tcaaccattc ggttcaatgc ttgtcttctt cctcttgcaa ctccggtctg cacaagcttt
     7261 ccttccggtg agacatgaag aagcggtttt aacttgatta ttgtgccgat ggtcgccact
     7321 actgccgaca aacgtccgcc tcttttcaaa tacatgagat cgtccactgt aaataaatgg
     7381 tcaagtctct ttttattctt ttccagccat tcttgaattt cttcaagtgt ttttccctgc
     7441 tttttcaacg tagccgcgtg ccaagtcaaa agacccagcc ccatcgaagc agccaaggaa
     7501 tctacggcta caactttccg accggggtat tccttttcca aattgggtcc ggcctggacg
     7561 gcggatgcgt aagtgccgga aagcgcgctt gaaaaggcga tgtataaaac atcaagccct
     7621 ttttcaagat atttccggaa aatatcatcg aattcctggg ggttaagctg tgatgtcgac
     7681 gacgttttct tgtttctaat caagtcatag aagttttttg tcgtcatttc cctttcgtcg
     7741 ggatagtttc gatagatctt atcatctacc atgaaactca tcgggattac ttccactccg
     7801 atttttttga ccattgccag agaaagatcg gaacaggagt cggtaaaaag tacaaaatca
     7861 gccatttctt atcctccgta tgtcataata ctatcaataa tgccaaaacc agggctccta
     7921 caggaatcaa tgttccgtaa acaagttccc ggggactatg tcttccaagg tacaaagacg
     7981 accatatgac aaagccaagg aagatcgtta atccgagata tccggggccg ccgaaatata
     8041 ccatagccgc aatcggcccg gcaacgccgc acatatggcc gctggctttt atctttagca
     8101 caaaactaga aaaagcaatg gtcgcgccag acaaaagata tgttaggtag aaaatttttt
     8161 gaatatcagt gaaattaaac acaaatgtta ttacagctcc gacaacatag ccaatgactg
     8221 aaaatacaat cgcggttttt ctttctcccg cccgcgtgtc ttccttatcg attttaaata
     8281 tccactgggt cggataggca agcaaaggca gaagcgacag cacggtgaca ctgaaaagat
     8341 attcccacac tgtctggaaa gattctggaa caaacaaata caatgtggat acgaaaataa
     8401 gcgccacgat cggggcggtg ctgattatac ggataacttt tgctaacttt ttcttcatga
     8461 ttttcgcctc ctgcccttta tgatatacgc attttgcata ttttttcttc tacaatcaac
     8521 gaaggcgact atattattct tatttcgctc agtagagaag cgaaaaatca ctccactcag
     8581 ggtagaatta cataaaacat gttgatattt ggcggttcat atgctatact cgctgtatga
     8641 taaacgaaaa cactttgcgc gaaacaatcg ccaaaaattt gaccaggtac cgtaaagcca
     8701 acaatctcac ccagcttcag ttggctgaaa agctcaatta ttcggataaa tccatctcta
     8761 aatgggaacg tggagaaggt attcccgatc ttttagttct ggcatccatc gcagatcttt
     8821 acgatatcac aatcaatgat attgtcacag atcgcaaaaa actcgcccat cagatacatg
     8881 ctttcaataa aatcatgata tcgctcattt ccttcaccgg tgtctggttt ttggcaacca
     8941 tcattttcgt aacacttcgg ttatttgttc cggaactgac cgatcgggct tggatgtcgt
     9001 tcatctattc cattccgatt tccctgatcg tcttattggt gtttgccagc atctggggca
     9061 aaagaatcca tttattcttc tgcgtttctc ttttgtcatg gtccgttccg ctggctttgt
     9121 tcctttcctt cgattatctc aaccttgacc tttggctgtt gttcatcgcc attatccctt
     9181 tacaagtaat aacgattatg gcgttcattc tcaaatcaaa aaaaacaaag agttgacaaa
     9241 agccaactct ttttttatga aaattacagc gggtttttaa acccgttttt atttttttag
     9301 cgtttgtctc tttccacatt cgaacgcttt atccaacg
//
LOCUS       DAOPJL010000037         9315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_7794_length_9315_cov_10.705724, whole genome shotgun sequence.
ACCESSION   DAOPJL010000037 DAOPJL010000000
VERSION     DAOPJL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7794_length_9315_cov_10.705724"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLP02_05090"
     CDS             complement(<1..224)
                     /locus_tag="PLP02_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42851.1"
                     /translation="MSDDLTNFVERSLMKTFKGAIYSKFCQAIDDYDLISEGDKIGVA
                     ISGGKDSLLMAKLFQAYEKYGSVKFEAVYFT"
     gene            391..774
                     /locus_tag="PLP02_05095"
     CDS             391..774
                     /locus_tag="PLP02_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42852.1"
                     /translation="MANFIWASKKPLDDMATLYESNITLNKSATAYFETAYVVLLGLD
                     KENLLIAVKPVTKEEIGLGYVPEEQQHNITVKSSYSRICNKLFMQEISDLLHLDFSDN
                     QSYKFKADWSEKDKALLINLKQKEV"
     gene            780..1229
                     /locus_tag="PLP02_05100"
     CDS             780..1229
                     /locus_tag="PLP02_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPF42853.1"
                     /translation="MFLTIDPETTMILIWVAIFVLAGIIEASTMDLSSIWFSAGALFS
                     LIIALIWPGSFTAQIVVFLVVSILSLLVLRPLFHKYIRKNEIKTNADRLVGKIAVCTK
                     AIADGDRGEVKIEGKIWTSISNENIAVGEKVEVLAIDGVKLVVKKSQ"
     gene            1226..1894
                     /locus_tag="PLP02_05105"
     CDS             1226..1894
                     /locus_tag="PLP02_05105"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPF42854.1"
                     /translation="MNAGFWKRAFSSVVDASLILLIIYLTFLAFGRKIITNQITNFDN
                     IYSTYQEISSASNANLSLLADEYNAAIVNADGDSDLEDAAQATYVAKKAVISDQRILD
                     IAPYNTALTMFLLYISYYYIVGFLLLLTVYVLAMKGYTLGRRLMQVKLEGPVHAASIF
                     FHDVILKYFFVAIALMLDMRIGILFIATFLIIDLITISFTGKKAALRDMILKMRVVTT
                     GYKY"
     gene            1923..2888
                     /locus_tag="PLP02_05110"
     CDS             1923..2888
                     /locus_tag="PLP02_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPF42855.1"
                     /translation="MFSFVFGGIGTGGIIALVSAAVIILIYLGARIKIVPQATQYVIE
                     RWGSYYTTWNAGFHFLFPFLDRIRKTYNARGIMVDSITLKEQVLDFPPQAVITKDNVT
                     MQIDTVVFLQVTDAKQYVYGAENPGLLIEQLTATTLRNIIGELELDQTLTSRDLINER
                     MRRILDEATDPWGIKINRVELKNIIPPKEIREAMEKQMRAERERRESILISEGKKASN
                     ILEAEGEKAAAILRAEAKKEAAIREAEGQAAAILKVQEATAKGLELIKSVKADGAVLT
                     LEAYRAMEKVSEGQATKIIIPSEMQGIVGLASGLVEALKDPKDKK"
     gene            complement(3042..4430)
                     /gene="asnS"
                     /locus_tag="PLP02_05115"
     CDS             complement(3042..4430)
                     /gene="asnS"
                     /locus_tag="PLP02_05115"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPF42856.1"
                     /translation="METTLMVLKEDYQTFAGQTVVLQGWIRNNRAQKEFGFIDLHDGT
                     CFDTAQIVYEEKNIDGFKDIQKYRIGSSIEVEGIIVATPNAKQPFEIKAERIVLIGDS
                     PENYPIQPKRHTREFLRENAHLRVRTNLFQAVFRIRNTVSYSIHKFFQEQGFIYVNTP
                     IITGSDAEGAGEMFRVTTIDMDNPPLNDDKKVDYGQDFFGRSANLTVSGQLQAEIFAM
                     AYRNVYTFGPTFRAENSNTQRHASEFWMIEPEIAFADLDKDMALAEAMIKYIIAEVLS
                     ECAQEVDFCDRFVESGLKDKLTKVLNSTFKSVKHKDAIDILLTSKEKFAIEPEQGQDL
                     ATEHQKYLTKYFDGPVFVTDWPKDIKAFYMRLNDDGKTVAAADLLVPGAGEIIGGSQR
                     EERLDVLEKRMAEMHVEEKPLWWYLDLRRYGGAPHAGFGLGLARMVMYVTGVDNIRDV
                     IPFPRTPKNCEF"
     gene            complement(4415..5116)
                     /locus_tag="PLP02_05120"
     CDS             complement(4415..5116)
                     /locus_tag="PLP02_05120"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPF42857.1"
                     /translation="MIIEELYSHKTMDKEVDRKPRYNQRPVKETYWGISAPTIRNVAK
                     KYQNEITEAKLDEMLKSRVHDVRTCALVIMNGKMKKATLEEQRRLARQYSKHLDKIDN
                     WDLVDISAPYILGEYTRNTQDFTLLFSYATTDDVWHRRISIVSTLTLVRHDQYDVSLK
                     IAEILLEDKEDTIHKACGWVLREVGKHNRELLDDFLEKHYEKIDRTTLRYAIERHEEN
                     IRQMILKGDFTWKQH"
     misc_binding    complement(5154..5358)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5427..6377)
                     /locus_tag="PLP02_05125"
     CDS             complement(5427..6377)
                     /locus_tag="PLP02_05125"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPF42858.1"
                     /translation="MAVFHGSKVKIFALSSNKKLAQEIADYIGIPLSQSEVTRFADGE
                     LSINIPETVRGHKVFVIQSTNPPVNEHIMELLIMIDALKRASAREINVVIPYYGYSRQ
                     DRKVKARQPISAKLVANLIQIAGATRVICMDLHAAQLQGFFDIPIDNFRSMPLISKYM
                     KDKNLQDIVVVSPDHGGVTRARTLADVLSAPIAIIDKTRPEPNVAEVMNIIGKVKGKN
                     CILIDDMVDTAGSVSAASKALQEAGAKDIYCCCTHPLLSGNAIKLIMDSPILELVCTD
                     TIELPEEKYFPKLVQLSVCELLGQGIINIIDDQGVSTLFQ"
     gene            complement(6487..6765)
                     /gene="spoVG"
                     /locus_tag="PLP02_05130"
     CDS             complement(6487..6765)
                     /gene="spoVG"
                     /locus_tag="PLP02_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HPF42859.1"
                     /translation="MQITEVRAKRVNGDNRLVGIAAITIDECFVVHELRIIEGKNGLF
                     VAMPSRKMPNGEFKDVAHPINTETRSEIEKAVLEAFAKLPELKDAKEE"
     gene            complement(6833..7684)
                     /gene="ispE"
                     /locus_tag="PLP02_05135"
     CDS             complement(6833..7684)
                     /gene="ispE"
                     /locus_tag="PLP02_05135"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPF42860.1"
                     /translation="MVTEKAYAKVNLFINVLDAKNNGYHNLKMINAKIDFYDTIRISK
                     IKSAGVALIESNDLFLSNQNNIVQDIAEYMVEHYLPDEGIKIEIEKKIPFGAGLAGNS
                     TDAAAIIRGVDKLLELHLSFTEMVEIGMKYGADIPYCLVDYPAIVEGEGEKITKIDFN
                     FSGCQLLLLNPRVYVATKDIFTIGNRKGFDNSDDQKIMKAIAKKDINKFAEAMHNALQ
                     KISVENNSDVAEAEKTLTKNFGTTGLVMTGSGSTFIKLLTGENQNVEDFMAKYKKKYF
                     VEIYNFL"
     gene            complement(7689..8390)
                     /locus_tag="PLP02_05140"
     CDS             complement(7689..8390)
                     /locus_tag="PLP02_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42861.1"
                     /translation="MKKRKRLESSKIVYFIFVGIVIGELIASIFSVIFANSSDTREAG
                     LSNIFLSVLAIVLFSVPWLIESRFKLDIPNYLEILVLSFLFASIVLGNIHNFLVDVQG
                     YDKLLHTVSGITISVIAFEIIHFINLSKPMEARMNPVMTAVFAFTFSVTLLVLWEFYE
                     FFIDTLSYNINNETLRNMQRYQWDNTSTIFPQDYGLVDTMLDLIVGTVGAILVSVSGG
                     FWLYSKEKKRLKKEA"
     gene            complement(8451..8783)
                     /locus_tag="PLP02_05145"
     CDS             complement(8451..8783)
                     /locus_tag="PLP02_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HPF42862.1"
                     /translation="MRKKYVDRGLIKWGAFDALVGYRPLLEAMKYRMGKKEKPAVSED
                     MLEEMNRILDFALCKNKEINVNYYSDGYMRSTFGNVIKMDKDKKKIFMSTGEIIDFDD
                     ITTIGTEK"
     gene            complement(8776..>9315)
                     /locus_tag="PLP02_05150"
     CDS             complement(8776..>9315)
                     /locus_tag="PLP02_05150"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HPF42863.1"
                     /translation="DMSLIQDKTKIKPFSKSYGTGQVLFRDYYKPDIYQIMLEMADDV
                     CRRLRTSNKQARTVYFGIGYGKDNGGGFSGSKTMERPTANASLIYDEFVRIFDKHYEE
                     DLPIRRVQIAVANLVNSEDLQLSLFEDPEKLVKEQKVAKAIDGIKNRFGKNSVNRASS
                     ELDGSTVKDRNKMIGGHHA"
BASE COUNT         2444 a         2123 c         1886 g         2862 t
ORIGIN      
        1 gtgaaataga cggcttcgaa ttttacgctg ccgtatttct catatgcctg aaatagtttt
       61 gccattaata acgagtcttt tccgccgctt atggccacgc cgattttatc tccttcggaa
      121 atgagatcat aatcgtcaat cgcctggcaa aatttcgaat aaatcgcccc tttgaatgtt
      181 ttcatcaggc ttctttctac aaaattcgtc agatcgtcac tcattgtgat caccgcctta
      241 tcaatgacta ttatacccgc aagaagtgat tttgtaaact tgtggtaatt atatataatc
      301 tcaaataatc ataaataatt cttgataatc acaaaataat gtgatataat tacatataga
      361 aaaaaatcaa catataatgg aggttggatt atggcgaatt tcatatgggc atcgaaaaag
      421 ccgcttgacg atatggccac attgtacgaa tcaaacatca cccttaacaa atcggccacc
      481 gcttatttcg aaaccgccta tgtagtcctg ctcggtctcg acaaggaaaa tttgctgatc
      541 gcggtcaaac cggtcaccaa agaagaaatc ggacttggct atgttcccga ggaacaacaa
      601 cacaatataa cggtaaaatc gagttattca cgcatttgta acaagttgtt catgcaggaa
      661 atttctgatt tgcttcatct ggatttcagt gataaccaat cttataagtt caaagccgac
      721 tggtcagaaa aggacaaagc tttgcttata aaccttaagc aaaaggaggt ttgaagcaca
      781 tgtttctcac aattgatccc gaaacaacaa tgattcttat ttgggtagca atcttcgtat
      841 tggccggaat catcgaggca tctaccatgg atttgagcag catctggttt tctgccggtg
      901 cccttttctc tttgataatc gctttgattt ggccgggcag ctttactgcg caaatcgttg
      961 ttttcttggt tgtatccatt ttatcgcttt tggtactgag accgttattt cataaataca
     1021 tccgcaaaaa tgaaatcaaa acaaacgccg atcgtttggt cggaaaaatt gcggtatgta
     1081 ctaaagccat cgccgacgga gaccggggcg aagtaaaaat tgaaggcaaa atctggacct
     1141 cgatttccaa tgagaacatc gctgtcggag aaaaagtgga ggtcttggcc attgacggag
     1201 tcaaattggt ggtcaagaag tcgcaatgaa cgcgggattt tggaaacggg ctttttcctc
     1261 cgtcgtggac gcgagcctga tcttactgat aatttacctg acgttccttg ccttcggccg
     1321 caaaataatt acaaaccaaa tcacgaattt cgataacatc tattccacat atcaagaaat
     1381 cagttcggca agcaacgcca atttgtcatt gttggccgac gaatataacg ccgcaatagt
     1441 caatgccgat ggagacagcg acttagaaga cgccgctcaa gccacttatg tggcaaaaaa
     1501 agcggtcatc agcgatcaaa gaattcttga catcgctcca tataacaccg ctcttaccat
     1561 gtttttgcta tatatctcgt attattatat tgtcggattc ttgctccttc tgaccgttta
     1621 tgtgttggct atgaaggggt acaccttggg ccgccggttg atgcaggtca aactggaggg
     1681 tccggttcac gcggcatcca tctttttcca tgatgtgatt ctcaaatact tcttcgtggc
     1741 aatagcacta atgcttgata tgcgaatcgg tatactgttt atagcaacct tcttgataat
     1801 tgatttgatt accatttcct ttaccggaaa gaaggccgct ttgcgtgata tgattttaaa
     1861 aatgagagtt gtaactaccg gatacaaata ttaaaaacca tatattttaa ggaggtatca
     1921 ctatgttttc atttgttttt ggaggcattg gcactggagg aatcatcgcc ttggtctccg
     1981 ccgcagtcat cattcttatt tatcttggcg ccagaatcaa gatcgttcca caagccacac
     2041 aatatgtcat tgaacgctgg ggaagctact ataccacatg gaatgccggt tttcatttct
     2101 tgtttccgtt cctggatcgc atcagaaaaa cttataatgc cagaggaata atggtcgaca
     2161 gcatcacttt gaaagaacaa gttctggatt tcccgccaca ggccgttatc accaaggata
     2221 acgtaaccat gcagatcgac accgttgtct ttctccaggt aacagacgcc aaacaatatg
     2281 tgtacggcgc cgaaaacccc ggattgttga tcgaacagct taccgctacc acgcttcgta
     2341 acatcatcgg cgaactggaa cttgaccaaa ccctgacaag ccgcgatctc atcaacgaaa
     2401 gaatgagaag aatccttgat gaagccaccg atccttgggg catcaagatc aaccgcgtcg
     2461 agttgaaaaa catcattcca ccgaaagaaa tccgtgaagc catggaaaag caaatgcgtg
     2521 ccgaacgtga acgccgcgaa tccattctga tttccgaagg taaaaaagct tcgaacatcc
     2581 tggaagccga aggcgaaaag gccgccgcga tcttgcgtgc cgaagccaaa aaagaagctg
     2641 ccatcagaga agctgaagga caggctgccg ccattctgaa agttcaggaa gcaacggcaa
     2701 aaggtctgga actcatcaaa agcgtcaaag ccgacggcgc cgtactaacc ttggaagcct
     2761 accgcgccat ggaaaaagta tcggaaggac aagccactaa gattatcatc ccttccgaaa
     2821 tgcagggtat cgtcggtctc gcatcgggac ttgtcgaagc tctcaaagat ccaaaagaca
     2881 agaagtaata tctcggatct aacaatcttt atcgcttcgc gcaaacagct ttatactcaa
     2941 aattccgctt tgtaaccaga taaaacagat aattataatt atcgattcca tacaaataaa
     3001 accggatttc tttcattatg aagtccggtt ttttgtttaa attagaattc acagtttttt
     3061 ggtgttctcg ggaacgggat gacatctctg atgttatcta cgcccgtcac atacatcacc
     3121 atgcgcgcaa gtcccaaacc gaatccggca tgcggtgccc ctccgtatct gcggagatct
     3181 aaataccacc agagcggctt ttcttccaca tgcatttccg ccatgcgttt ttcaagcacg
     3241 tcgagtcgtt cctctctttg ggatccaccg ataatttcac cggctcccgg cactaaaaga
     3301 tccgctgcgg cgacggtttt gccatcatcg ttcaaacgca tatagaaggc ttttatatcc
     3361 tttggccaat ccgttacgaa aaccggccca tcaaaatact tggttagata tttttggtgt
     3421 tcggtggcca aatcttgccc ttgttctggc tcaattgcaa atttttcttt acttgttaac
     3481 aaaatatcta tggcatcttt atgcttgaca gacttgaatg tggagttaag aactttagtc
     3541 aatttatctt tcaatccact ttcgacaaag cggtcacaga aatccacttc ctgggcacat
     3601 tccgaaagca cttcggcaat gatatacttg atcattgctt ccgctaaagc catatccttg
     3661 tccagatcgg caaatgctat ttcgggttca atcatccaaa attccgatgc gtgtctttgc
     3721 gtgttggagt tttcagcccg gaatgttggt ccaaaagtgt agacgtttct atacgccatg
     3781 gcaaatattt cggcttgaag ctgtccggat acggtcaggt tggccgatct tccgaaaaag
     3841 tcctgtccgt agtccacttt tttatcatca ttcaaaggag gattgtccat atctatggtt
     3901 gtgactctga acatttctcc agcgccttcg gcatctgatc cggttatgat cggcgtgttt
     3961 acgtatataa atccttgttc ctggaaaaat ttatgaatac tgtaagaaac cgtattgcga
     4021 attcggaaga cagcttgaaa taaattggtt cttacgcgca aatgcgcgtt ttcccggaga
     4081 aattctcttg tgtgcctttt tggttgtatg ggatagtttt ccgggctgtc tccgattaaa
     4141 actattcttt cagctttgat ttcaaacggt tgtttggcat tcggggtggc aacgataata
     4201 ccttcaactt cgatcgatga tccgatccgg tatttttgaa tatccttaaa tccgtcgatg
     4261 tttttttcct catatacgat ctgagctgta tcaaaacaag tgccgtcatg cagatcgata
     4321 aacccgaact ccttctgggc tcggttattt ctgatccacc cttgcaaaac caccgtttga
     4381 ccagcaaacg tttgataatc ctcttttaat accatcaatg ttgtttccat gtgaaatccc
     4441 cctttaaaat catttgtcta atgttttcct cgtgtctttc gattgcgtaa cgcaaagtcg
     4501 tacgatctat cttttcatag tgcttctcca aaaaatcatc gagtagttcc cggttgtgct
     4561 ttccaacttc tctcagaacc catccgcaag ctttatgaat tgtgtcctct ttgtcctcaa
     4621 gcaagatttc cgctatcttc aaagatacat catattgatc gtgtcttaca agggtcaagg
     4681 tggacactat gcttattctt ctatgccaaa catcatcggt ggtagcgtag gaaaacaata
     4741 aagtaaaatc ctgtgtgttg cgagtgtatt cgccaagaat gtaaggcgcc gatatatcaa
     4801 ccaaatccca attatcgatc ttgtcgagat gtttagaata ttgtctggct agccttcttt
     4861 gttcttcgag tgttgctttt ttcattttcc cgttcatgat gacgagagca caagttctta
     4921 catcatggac tctgcttttc aacatctcgt ccaattttgc ctctgtgatt tcgttctggt
     4981 attttttggc aacgtttctt attgtcggag cgctgattcc ccagtaggtt tcttttaccg
     5041 gcctttgatt gtatcttggt tttctgtcta cttctttatc catggtttta tgggagtata
     5101 gttcttcgat aatcattatg ttacccctcc gtataaataa aaaatcgtcc tttttcaagg
     5161 acgaataaaa atccgtggta ccacctttgt tccggctttg ccggcgctcg atatccttaa
     5221 cgcggaatga cgtacaggtc tacttgattt cttctgtctc tcccggtgtt tttcgaaatg
     5281 tctccgtcac aaggcttcca ccgccccttg ctcgctttgg acaaaacgac attctactcg
     5341 tccgttcatc gatgtaccta tattatacat catgtttgct ttctttgtaa acacaagaaa
     5401 aaaacatcgg cgtttgccga tgctttttat tggaaaagag tggacactcc ttgatcgtcg
     5461 ataatgttta taataccttg tcctaagagt tcgcacacag ataattggac cagttttgga
     5521 aaatattttt cttccggcaa ttcgatcgtg tcagtgcata caagttccaa aattggcgag
     5581 tccatgatca atttgatggc gtttccggaa agaagcggat gcgtgcagca gcagtagata
     5641 tccttggctc cggcttcctg cagggctttg gatgccgcag acacagaacc ggctgtgtcg
     5701 accatatcgt cgatcaaaat gcaattcttg cctttgacct taccgatgat gttcatcact
     5761 tcggcgacat tgggttccgg gcgggttttg tcgatgatgg cgattggcgc ggacagaacg
     5821 tctgccaagg ttctcgccct agtaacgcca ccgtgatccg gagacacgac gacgatatcc
     5881 tgcaaattct tatctttcat gtatttggaa atgagaggca tcgaacggaa attatcgatc
     5941 gggatatcaa agaacccctg aagctgagcg gcgtgaagat ccatacagat gacacgcgta
     6001 gccccggcga tttgaatgag gtttgcgact agtttggcgg aaatgggttg tctggctttc
     6061 actttccgat cctggcgtga atatccataa taaggaatga ctacgtttat ttccctggcc
     6121 gacgcgcgtt tcagcgcatc gatcatgatc aggagttcca tgatgtgttc gttcaccggc
     6181 ggattggtcg attggatgac aaacactttg tgtccgcgga cggtttctgg aatattgatg
     6241 ctcagttcgc cgtcagcaaa acgggttact tccgattgac tcaaagggat accgatgtaa
     6301 tcggctattt cctgagccag ttttttgttg gaactcaaag cgaagatttt gacttttgat
     6361 ccgtgaaaga ctgccatttc tttcctccgt ggttctttat tttgatgaca cagatattat
     6421 acaacataaa aagcaaatta aaaagtgcca actttcccgg tcggcacttg cgtttatcaa
     6481 aaagatttat tcctctttgg catccttgag ctccgggagt ttggcaaagg cttctaaaac
     6541 ggccttctct atttcgctcc ttgtttcggt attgatagga tgggcgacgt ccttgaactc
     6601 cccattaggc attttgcgac ttggcatcgc cacaaagaga ccgttcttcc cctcaatgat
     6661 cctcagctca tgaacaacga agcactcgtc aatagtaatg gcggcaattc ctactaagcg
     6721 attatcccca ttgactcttt ttgcacgcac ttccgttatc tgcatattac cacctcttta
     6781 gatgattata tcatgcgatt tttacttttt ctatacgttt aacgaaattg cttcacaaaa
     6841 aattgtaaat ttcaacaaaa tatttctttt tgtatttagc cataaaatcc tcgacattct
     6901 ggttttcgcc ggttaacagt ttgatgaaag tcgaacctga tccagtcatt accaaaccgg
     6961 ttgtcccgaa gttttttgtg agtgtttttt ccgcttccgc aacatcggaa ttgttctcga
     7021 cggaaatttt ctgtaaagcg ttatgcatcg cctcggcaaa tttgttaata tcttttttgg
     7081 ctatggcttt cattatcttc tggtcatcgg agttatcaaa ccctttacga ttaccgatgg
     7141 tgaaaatatc ttttgtcgcc acatatactc gcgggttcaa aagtaagagc tggcatccgg
     7201 aaaaattaaa atctatcttg gtaatctttt ccccttcgcc ttcgacgatc gccgggtaat
     7261 caaccaggca atatggtatg tcggctccat atttcatacc gatctccacc atctctgtaa
     7321 aacttagatg gagttccagc aatttgtcca cgcccctgat gatggccgcg gcatctgtgg
     7381 aatttccggc taatcccgct ccgaacggaa tctttttttc aatttcgatt tttatccctt
     7441 cgtcgggaag atagtgttcc accatgtatt cagcaatgtc ttgaacgata ttgttctggt
     7501 tggacaaaaa gagatcgttg gattcgatca aagccactcc ggccgatttt atcttggata
     7561 ttcttatcgt atcgtaaaaa tcgattttgg cattgatcat tttcagattg tggtatccgt
     7621 tgtttttcgc atccaggacg tttatgaaca gattgacttt agcgtatgct ttttcggtaa
     7681 ccatgttgtc aggcctcctt tttcagtctc tttttctctt tggaataaag ccagaatcct
     7741 cccgacacag aaaccaatat ggcgccgacg gttccaacaa tcaaatccag cattgtgtcc
     7801 accaatccgt agtcttgggg aaaaatggtg ctggtgttgt cccattgata tctttgcatg
     7861 ttacgcaacg tttcgttatt tatgttgtat gacaacgtgt cgataaaaaa ctcgtagaat
     7921 tcccacaaca caagaagcgt cacggaaaac gtgaaggcga acaccgcagt catcaccggg
     7981 ttcatccgcg cttccatagg tttgctaaga ttgatgaaat gaatgatttc aaaagcaatc
     8041 accgagattg ttattccgct taccgtgtgt aacaatttat cgtatccctg gacgtcgaca
     8101 agaaaattat gaatgtttcc gaggacgatc gatgcaaaga gaaaactcaa aaccaggatt
     8161 tccaaataat ttggtatatc gagtttgaac cttgattcta tcaaccaagg aaccgaaaac
     8221 aaaacaatgg cgaggacaga tagaaatatg ttggaaagtc cggcctctct tgtgtctgac
     8281 gaattggcga atatcacact aaagattgac gctatcaact caccgatcac gattccgaca
     8341 aatatgaaat atacaatttt cgaactttcc aggcgttttc ttttcttcat cgcttttctc
     8401 cttttattgt agcgttttac tttgtggtat ttcaagttcc cagctgtttt ttatttttcc
     8461 gtcccgattg ttgttatatc atcgaaatcg attatttccc cggttgacat gaaaatcttt
     8521 tttttgtctt tgtccatttt aattacattg ccgaaagtgg aacgcatgta tccgtcgcta
     8581 taatagttta cattgatttc cttgtttttg caaagcgcaa aatccagtat gcgattcatt
     8641 tcttccagca tgtcttcgct gacggccggt ttttcttttt ttcccatccg atatttcatc
     8701 gcttcaagca acggtctgta acccaccaac gcgtcgaacg cgccccattt gattaagccc
     8761 cggtccacat attttttacg catgatggcc tccgatcatt ttatttctgt ctttgacggt
     8821 agatccgtca agttcgctgg aagcccgatt gacggaattt ttaccaaaac ggtttttgat
     8881 gccgtcgatg gctttggcta ccttctgttc cttgaccagt ttttccggat cctcaaacag
     8941 actcagttga agatcttcgc tatttacaag attggctacg gcgatctgaa cgcgtcttat
     9001 gggaagatct tcttcatagt gcttgtcaaa aatccgaaca aattcgtcat agataagcga
     9061 tgcgttggcc gttggtcgtt ccatagtttt gctgccggag aatccaccgc cgttgtcttt
     9121 tccgtatcca attccaaaat agaccgttct ggcctgcttg ttgcttgtgc gcaaacgccg
     9181 gcaaacatca tcggccattt cgagcatgat ttgatatata tccggtttat aataatccct
     9241 gaataaaact tgtccggttc cgtagctttt actaaacggc tttattttgg ttttgtcttg
     9301 gatcagactc atatc
//
LOCUS       DAOPJL010000038         8763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_8490_length_8763_cov_10.320854, whole genome shotgun sequence.
ACCESSION   DAOPJL010000038 DAOPJL010000000
VERSION     DAOPJL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8490_length_8763_cov_10.320854"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="hprK"
                     /locus_tag="PLP02_05155"
     CDS             <1..702
                     /gene="hprK"
                     /locus_tag="PLP02_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPF42864.1"
                     /translation="EPPAFVFSTNVAVPKLFLELGDEFDIPVLKSSYATTSLFSELFS
                     FLQAKLAERKALHGVLLDINGVGVMIVGESGLGKSEVALELIRRGYMLVADDMVEIYQ
                     VEKGVLIGEAPELLKKYLEIRGIGIVNIIYLFGVKAYRETKKISLMVELEKWDDYKEF
                     DRLGTKQESRRIFDTDVPYVGLPITEARNTATLVEAAAYAFKSRSLGYQSSDEFSENL
                     SRKIAENGKRNGEEK"
     gene            699..1979
                     /gene="lgt"
                     /locus_tag="PLP02_05160"
     CDS             699..1979
                     /gene="lgt"
                     /locus_tag="PLP02_05160"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPF42865.1"
                     /translation="MKKAIKFGTILIVAVLAIFLTGCQNTDDSQTTYTVTFVDYYGNE
                     ITSFTCDKGEVCPIVTPDDPLDIGDRYFTRWSVWESEYADINSDTTIKPIYTLDNVAF
                     TVFTREVMFYSLFILTGIVVALLMGLRECKRLGIKQDDLIDGFLWTVPVAIIGARLWY
                     VVFEWDQFVGDTFSETMAMIFGFHNGVWFDFHTYGDYQNFGFAGLAIHGALFTAILCV
                     IIHTKVRKMDLMKVLDMMAVGFIIAQAFGRWGNFFNQEAHGGLVGGLLANGDPALTVE
                     QQYNYLRYTLHIPEFIVNNMYIEQGLHEVVDGYYHPTFFYESTINLVGFAIMLVLRRL
                     KKAHFGDLFAFYLVWYGALRIFIEMMRTDPLEFELFGITFKSAIVTSSIMILIGILIS
                     VYTRIKRKDKTYGTIPGCFGCPKQDQNQIVTENA"
     gene            1996..2946
                     /gene="whiA"
                     /locus_tag="PLP02_05165"
     CDS             1996..2946
                     /gene="whiA"
                     /locus_tag="PLP02_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPF42866.1"
                     /translation="MSFSTDVKTELSSLKHLDCCNKAELSALFHVGGTVERSNNGMAI
                     EFQSTNLAVVRRVIGLVTQLYNDNYTLMSKKQTKLQKKDMYIVRLEPPADDILSDLAI
                     IDKSGMISHATDKELIKKECCKKAYLRGAFLAGGSINNPDTAAYHLEIQTFAEKAAEQ
                     IGHLANEFDLNAKIAKNRRGNIVYMKEAEKIADFLRVVDATNSLFTFEDNRIKRDFKN
                     SINRVINCDIANEKKAIEAANKQLEYIEVIEQNHRNDLPESIEQAIFLRKKYPESTLL
                     ELSYAAEEHYGNRISKSALNHRFRAIKDLALQIRIEEEGK"
     gene            2943..3290
                     /gene="acpS"
                     /locus_tag="PLP02_05170"
     CDS             2943..3290
                     /gene="acpS"
                     /locus_tag="PLP02_05170"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPF42867.1"
                     /translation="MITGIGTDIVRIDRIKEDMIDMILSGEERKMYETFGSEKRRREF
                     LAGRFAAKEALTKALSSVGLVATFRETIIINDENGQPVLKYPSWPEHNIHLSISHEND
                     YATAVCVLETKDM"
     gene            3369..3890
                     /locus_tag="PLP02_05175"
     CDS             3369..3890
                     /locus_tag="PLP02_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42868.1"
                     /translation="MATYRRGFKKTTPEQMLLRVIVGIIAVVFVFVIAAYLYASTYGS
                     WEYENYTHLTAYETLLTQEDENEDQLQDYVLYFYGSGCESCLDIKNKVMRYTDKINSD
                     STMVYFVDIENMDDEDEYLDTFLDDISKSTLTTPMLVVVANGDFYEWVSGTDDVVNLL
                     KQIKDGEYEPFND"
     gene            4003..6204
                     /locus_tag="PLP02_05180"
     CDS             4003..6204
                     /locus_tag="PLP02_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPF42869.1"
                     /translation="MEILSEELIAEVAANQNIKVTQVKAVLDLLGEGQTVPFIARYRK
                     EMTGSLDEECIRAIEKEYEYKVNFLKRKDDITRLIDEKGMLTEEVREAIKKAEKLVDL
                     EDIYRPYKEKKKTKATEAIKKGLEPLAVIILTYPSGKTLAELAAPFINETVPTAEEAL
                     EGAMYIIAENISDNAIYRKWIREYTFNNGTLVSTKKKDADDPYQTYKNYYEYEEPLKS
                     IKLYRILALNRAEKEKVVNVKITVETQAVLNYLERNFIGKKESECVELVKKAIDDAYK
                     RLIAPSIEREIRSDLSEKAEDQAIHIFSENLRSLLMQPPLKGKNVLGVDPAFRTGCKL
                     AVVDKFGKFLDKGVIYPHESKLGEKIDPVKVEASVKKVKSYLEKYDIDIIAIGNGTAS
                     RETEAFIAGVIKSAIRPVKYVIVSEAGASVYSASDLARQEFPDFQVEERSAVSIARRM
                     QDPLSELVKIDPKSIGVGQYQHDVSQNKLTDSLDFVVSTAVNQVGVDVNTASVSLLKF
                     ISGLSANVASNIVTYREKHGPFANREKIKDVPQMGPKTFEQAVGFLRILESDNPLDKT
                     PIHPESYETAKTILKTLSIKPEDIGSLGCQLKISGTNRDKLAASLGMNSITINDLLDA
                     FAQPTRDLRDQYPQPLLKSELLKLEDLRPGMELQGTVRNVVDFGAFVDCGIKEAGLVH
                     ISKMSKKYVKHPLEVVSVGDIVKVWVLSVDLERKRLQLSMLDPFESNTYVN"
     gene            6238..>7379
                     /locus_tag="PLP02_05185"
     CDS             6238..>7379
                     /locus_tag="PLP02_05185"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPF42870.1"
                     /translation="MRYDFLMAAILFAMGSFMFITVVFALTRRELLLGFVLLSLLALA
                     NGIFSVSYGGFILSDTESSMLIFTHFSYIAAPFISLIWFLISIQQRTKVRMLPMQKYW
                     PFLVVPVLVLCASLMYPWGPSTGTVWFQRLFFVSHSMETSASIGSGFSTLIFEKGSLY
                     FIMTAYDALLFLGAAINYYDTFQKTESVHRRDILILLITSLVAFGFLVYTLSSSNTAL
                     IGILPFASVAITILAMACLYKYEFFDLIPFAYRQLFQSVSVPIFIFDKSMTVISANDT
                     AKKYYGNIFNFRELMNIQDFNRIDSNFRDDLIKKGEHETFVNTNGDTRYFNARFEVLK
                     QDGKQSFGYLLQYVDITDHKKELARMENAATYDDLTKIFNRRVFYL"
     assembly_gap    7380..7479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7480..7680
                     /locus_tag="PLP02_05190"
     CDS             <7480..7680
                     /locus_tag="PLP02_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42871.1"
                     /translation="LAEKMRQELDVHIFEYNGRKIKTSASFGVSGSSGVVKKSFERYI
                     KDSDDGLYLSKSKGKNKVEIVL"
     gene            7762..8460
                     /locus_tag="PLP02_05195"
     CDS             7762..8460
                     /locus_tag="PLP02_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42872.1"
                     /translation="MAKPKYNYGWLLKFILAAILLGVGIYMVFADKVVYLITGVAIIV
                     FSLLRIVPLVKTLKTELLRTINIVEIVLDTLMGAFMLYVALSGKDLANEPVWGGIYKW
                     LLAIFFYLRGLVFFNSVVFLGEKTEIPKFWVHIGCLTLGAIIAVWPNFDYNVVGVFFL
                     LISITGAAYLGYDGYGGYRKYREYSKALNEGKKAAEKPAEEKEVPDPEIKKPEKEPEK
                     VPEKEPEEKETYIN"
     gene            8517..8607
                     /locus_tag="PLP02_05200"
     tRNA            8517..8607
                     /locus_tag="PLP02_05200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8551..8553,aa:Ser,seq:gct)
     gene            8670..8760
                     /locus_tag="PLP02_05205"
     tRNA            8670..8760
                     /locus_tag="PLP02_05205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8704..8706,aa:Ser,seq:gga)
BASE COUNT         2646 a         1736 c         2016 g         2265 t
ORIGIN      
        1 gaaccgccgg cattcgtgtt ttccaccaat gtcgccgtgc ccaaactgtt tttggaactg
       61 ggcgacgaat tcgatattcc cgtccttaaa agttcatatg cgaccacttc gctttttagt
      121 gaactgtttt cctttctcca ggcaaagttg gcggaaagga aagcccttca tggggttctt
      181 ctggacatca atggcgttgg tgtaatgatt gtcggggaga gcggccttgg aaaaagtgaa
      241 gtcgctttgg aactgataag acgcggatac atgctcgtag ccgacgatat ggttgaaata
      301 tatcaggtcg aaaaaggcgt gttgatcggc gaagcgccgg aacttctaaa aaaatatctt
      361 gaaatccgtg gaatcggaat cgtcaatatc atttatcttt tcggtgtcaa ggcttaccgg
      421 gaaaccaaaa agatttctct aatggtcgaa ttggaaaaat gggatgatta caaagaattc
      481 gaccgcttgg gaaccaaaca agaatcaaga cggattttcg acaccgacgt tccttatgtc
      541 gggttgccaa taaccgaagc cagaaatacc gcaaccttgg tcgaagcagc cgcatatgca
      601 ttcaagtcac gaagcctcgg ataccagtcg tcggatgagt ttagcgaaaa tctcagccgc
      661 aagattgcgg aaaacggaaa aagaaacggg gaagagaaat gaaaaaagcc atcaaattcg
      721 gaacaattct tatcgtggcg gtattggcta tttttcttac cggatgtcag aacaccgacg
      781 attctcagac tacgtacacg gtaacgttcg tcgattatta tggaaatgaa atcacatcgt
      841 tcacttgtga taaaggcgaa gtctgcccga tagtcacacc ggacgacccg cttgacatcg
      901 gcgatcgata tttcaccaga tggagcgtat gggaaagtga atatgccgat atcaattcgg
      961 atacgacgat caaaccgata tataccttgg acaatgtcgc ctttactgtt tttacaagag
     1021 aagtgatgtt ttattccctg ttcattttga caggaatagt ggtcgcgttg cttatgggct
     1081 taagggaatg caaacggctt ggcattaaac aggacgattt gattgacgga tttctttgga
     1141 cggttccggt agcaattatc ggggcccgat tgtggtatgt cgtatttgag tgggatcaat
     1201 ttgtcggcga tacattttcc gaaaccatgg ctatgatctt cggtttccac aacggggtat
     1261 ggtttgattt tcacacgtat ggagattacc agaacttcgg ctttgccggc ttagccattc
     1321 atggtgcttt gtttacggcc attctttgtg tgataatcca tactaaagtg agaaaaatgg
     1381 atctcatgaa agtcttagac atgatggcgg tcggtttcat aatcgctcag gctttcggac
     1441 gctggggcaa cttctttaat caagaagcac acggcggtct ggttggcggt ttgcttgcga
     1501 acggagatcc ggcgctcact gtcgaacaac aatacaatta tcttcgatat acattgcata
     1561 ttcccgaatt catcgtcaat aacatgtata tagaacaagg acttcatgaa gtagtggatg
     1621 gttattatca tcctacattc ttctatgaat cgacgatcaa tcttgtcgga ttcgcgatca
     1681 tgcttgtatt gagaaggttg aagaaagctc acttcggcga tttgtttgcc ttctatctgg
     1741 tttggtatgg tgcattgcgg atttttatcg aaatgatgag aaccgaccca ttggaattcg
     1801 aattattcgg aatcactttc aaatcagcca tcgtcacatc gtccataatg attttgatcg
     1861 gaatcctgat cagcgtctat accaggatta agcgcaaaga taaaacatac ggaaccattc
     1921 cgggttgttt cggatgcccg aaacaagatc aaaaccagat cgtaactgaa aacgcctgaa
     1981 attattggag aaaacatgtc attttccaca gatgtaaaaa ctgaattgtc cagtctaaaa
     2041 catttagact gttgcaacaa ggcggaacta tccgcccttt ttcacgtcgg aggaacggta
     2101 gaacgctcaa ataacggaat ggcaatcgaa tttcaatcaa ccaatctggc agtcgtcagg
     2161 agagtcatag ggctcgttac ccaactttat aacgataatt acaccttaat gagcaagaag
     2221 cagacaaaat tgcaaaaaaa ggatatgtat attgttagac tggaaccgcc ggccgatgac
     2281 attctttcgg atcttgcaat aatagacaaa tcaggaatga tttcacatgc taccgataag
     2341 gaattgatta aaaaagaatg ttgcaagaaa gcttatctac gaggcgcttt tcttgccggc
     2401 ggatccatca ataatccaga tacggcagct tatcatctgg agatccagac attcgcggaa
     2461 aaagcagccg agcagattgg tcatttggcc aacgaatttg atctgaatgc gaaaatcgca
     2521 aaaaaccgac gaggaaatat tgtgtatatg aaagaagcag aaaaaatagc tgactttctt
     2581 cgcgtcgtcg atgctacaaa ttcgcttttc acattcgagg acaaccggat caaacgcgat
     2641 ttcaagaatt cgataaaccg agtgatcaat tgtgacatcg ctaacgaaaa gaaagcgatc
     2701 gaggcagcga ataaacaatt agaatatata gaagtaattg agcaaaatca caggaatgat
     2761 ttgccggaaa gcatcgaaca agcgattttt ttgcgtaaga aataccctga atcgactttg
     2821 ttggaattga gttacgccgc cgaagagcat tatggcaacc ggatttccaa atccgctctc
     2881 aatcatagat tccgggcaat caaggatttg gccttgcaaa tcagaatcga ggaggaaggc
     2941 aaatgattac aggaatcgga acggacatag tcagaattga tcgtattaaa gaagacatga
     3001 tcgacatgat attatctggt gaagaaagaa agatgtacga aacatttgga tcggaaaaaa
     3061 gacgccggga attcttggct ggtcggtttg ccgcaaagga agccctgacc aaagctttgt
     3121 catctgttgg actggttgcg actttccggg aaacgataat tataaacgat gaaaacggac
     3181 aaccagtgct aaaatatccg agttggccgg aacataatat acatttgtcg atatcccacg
     3241 aaaacgatta tgcgacggca gtttgcgtgc tggaaacgaa agatatgtga aatatggaaa
     3301 gcaagaggaa aatagtgctt gcataaatca tgctttttcg gtaaaataaa taatgctgag
     3361 gtgaaaatat ggccacatac agaagaggat ttaaaaaaac aacgccggaa caaatgctgc
     3421 tcagagtgat cgttggaatc attgctgtag tttttgtgtt tgtgattgcg gcttatcttt
     3481 atgcgagtac ttacggatca tgggagtatg aaaactacac tcacttgacc gcatacgaaa
     3541 cactgttgac ccaagaagac gaaaacgaag atcaattaca agactatgtc ctttatttct
     3601 atggcagcgg atgcgaatca tgcctcgaca tcaaaaacaa agtgatgcgt tataccgaca
     3661 agattaattc cgactcaacg atggtgtatt ttgtggatat cgaaaacatg gatgacgagg
     3721 atgaatatct ggacacattc ctagacgaca tctcgaaatc gaccctgacg accccgatgt
     3781 tggtggttgt ggccaatgga gatttctatg aatgggtaag cggaactgac gatgttgtaa
     3841 atctgttgaa acaaataaaa gacggagaat acgaaccatt caatgattga caaacaaaaa
     3901 acccagaaat gggttttttt attttaataa tctaggataa atatttcttt ttgcctccta
     3961 atatgatata aatataatat gctaacggga ggcggagact agatggaaat attgtcagaa
     4021 gaattgattg cggaggttgc cgcgaaccaa aatatcaaag tcacgcaggt caaggccgtc
     4081 cttgaccttt taggcgaagg acagactgtg ccgttcattg ccagatacag aaaagaaatg
     4141 actgggtcgc ttgacgagga atgtattcgg gcaatcgaga aagaatatga atacaaggtc
     4201 aatttcctga aacggaagga tgacatcacc aggctgatcg atgaaaaagg aatgctaacg
     4261 gaagaagtac gggaagcgat caaaaaagcg gaaaagttgg tggacctgga ggacatttac
     4321 cggccttaca aagaaaagaa aaaaaccaaa gcgactgaag cgatcaaaaa aggattggag
     4381 cctctggccg ttataattct cacttatccg tccgggaaaa cattggcaga gcttgcagcg
     4441 cctttcataa acgaaacggt accaacggcg gaagaggcct tggaaggcgc catgtatatt
     4501 atcgccgaaa acatatccga caacgcaata taccgaaaat ggattcgcga atacactttc
     4561 aataacggga cgcttgtgtc gacaaagaaa aaggatgcgg acgatcctta tcaaacctat
     4621 aaaaattact atgaatatga agaaccgctg aaaagcatca aactctaccg gattcttgct
     4681 ttgaaccgag cagagaaaga aaaagtagta aatgtaaaaa tcaccgtgga aacccaagct
     4741 gttcttaatt atctggaacg taatttcatt ggaaagaaag aatcggaatg tgtagagttg
     4801 gtcaaaaagg cgatcgatga cgcatataag cgtctgatcg ctccctcgat cgaaagggaa
     4861 atcagaagcg acctttcaga aaaagcggaa gaccaagcga tccatatctt ttccgagaac
     4921 ctacggtcat tgctgatgca gccgccttta aaggggaaaa atgtgcttgg cgtcgatccg
     4981 gcatttcgca ccggatgcaa attagccgta gtggataaat tcggaaaatt tctcgacaaa
     5041 ggcgtgattt atccccatga atcaaaattg ggagaaaaaa tcgatccagt taaagtcgag
     5101 gcttcagtca agaaagtaaa atcctatctg gaaaaatatg atatcgatat aatcgccatc
     5161 ggaaacggaa ccgcttcccg ggaaaccgaa gcattcattg ccggtgtgat taaatccgcg
     5221 atccgaccgg tcaaatatgt catcgtcagc gaagccggag cttcggttta ctcggcttcc
     5281 gatcttgcca gacaggaatt tcccgatttt caggtcgagg agagatccgc cgtcagtatt
     5341 gccagacgca tgcaggatcc tttgagcgaa ctggtgaaga ttgatccgaa atcaatcgga
     5401 gtcggacaat accagcatga tgtttcccaa aacaaattaa ccgattctct tgatttcgtt
     5461 gtgtcgaccg cggtcaatca ggtcggcgtc gatgtgaata ccgcttcggt ctctcttttg
     5521 aaattcattt ccggcttatc cgcaaatgtg gcatcgaaca ttgtcacata ccgtgaaaaa
     5581 cacggtcctt ttgccaaccg cgagaaaatc aaggatgttc cgcagatggg cccaaagaca
     5641 tttgaacagg cggtcggatt tttgcgtata ctggaatccg acaatccgct tgacaaaact
     5701 ccgatccatc ctgaaagtta tgaaactgcg aagacaatat tgaagacatt gtccataaaa
     5761 cccgaagata tcggttctct cggatgccag ttgaagatat ccggaaccaa tcgcgataaa
     5821 ttggccgctt cgcttggaat gaattcaatt acgataaacg atttgcttga tgcatttgcc
     5881 caaccgacca gggacttgcg cgaccagtat ccgcaaccgc ttctgaaaag cgaattgttg
     5941 aaactggagg atttgcgtcc gggaatggaa cttcagggaa ccgtgagaaa tgtcgtcgat
     6001 ttcggtgctt tcgtcgactg cggaatcaag gaagcgggac ttgtgcatat atcgaaaatg
     6061 agcaagaaat atgtcaagca tccgttggaa gtggtttcgg tcggagacat cgtgaaagtg
     6121 tgggtgcttt cggttgatct cgagcgtaag cgactgcaac tatcaatgct cgatcctttt
     6181 gaatcaaaca catatgtaaa ctaatcacaa tgcttcaatc gacaaggagg gaaaaagatg
     6241 cggtatgatt ttctgatggc agccattctt tttgctatgg gatcgttcat gttcataacc
     6301 gttgtctttg ctcttacaag acgcgaattg ttgcttggtt ttgtattatt aagccttttg
     6361 gctttggcta atggtatttt ctcggtgtcc tacgggggat tcattttgag cgataccgaa
     6421 tcatcaatgc tgatcttcac tcattttagt tatatagccg cgcctttcat ttcgcttatc
     6481 tggtttctta tttccatcca gcaaagaaca aaagtcagaa tgcttcccat gcaaaaatac
     6541 tggccgttcc tagtagtccc cgtcttagtc ttatgcgcta gtctgatgta tccttggggc
     6601 cctagcaccg gtacggtctg gtttcagcgt ttgttttttg tctcccacag catggaaaca
     6661 agtgccagta tcggatcggg attcagtacg ctgatatttg aaaaaggcag tctttatttc
     6721 attatgacgg catatgatgc gttgttgttc ctcggagcgg ccatcaacta ttatgatact
     6781 ttccagaaaa cggaatcggt gcatcgtaga gacatcctaa ttcttttgat cacatctctt
     6841 gttgcattcg gatttcttgt gtataccctg tcgtcatcaa acaccgcgtt gatcggaatt
     6901 cttcccttcg cctcggttgc gattacgata ctagcgatgg cttgccttta taagtacgaa
     6961 tttttcgacc tgattccatt tgcataccga caacttttcc aatcggtatc ggtgcctatt
     7021 ttcatattcg acaaatcgat gaccgtgatc agcgcaaatg acacggcaaa aaaatattat
     7081 ggcaatatat ttaatttccg ggaactgatg aatattcagg atttcaatcg gattgattcg
     7141 aactttcggg atgaccttat caaaaaaggc gagcacgaaa cctttgtgaa cacaaatggg
     7201 gataccagat atttcaatgc cagatttgaa gtcttgaaac aggacgggaa acagtcgttc
     7261 ggatatctgt tacagtatgt cgatatcacc gaccacaaaa aagaattggc caggatggaa
     7321 aacgcggcaa cttatgacga tctaacaaaa atattcaatc gccgggtttt ttatctgaan
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tggcggaaaa aatgcgtcag
     7501 gaacttgatg tgcatatttt tgaatacaac ggacgcaaga taaaaacaag tgcaagtttc
     7561 ggcgtttccg gatccagcgg cgtagtcaaa aaatcatttg aaagatatat caaggattcg
     7621 gatgacggtt tgtatctatc taaaagcaaa ggaaagaaca aagtggaaat cgtgctttga
     7681 aaagaaatgc aaactgttta ttttcaatag aaaaagcgct ataatattaa tggttaaacc
     7741 caatattagg agggatgaaa catggctaaa ccaaagtata attacggatg gttgctgaaa
     7801 ttcatactgg ctgccatttt gctaggagtc ggtatctaca tggtttttgc cgacaaggtt
     7861 gtatatctga tcaccggtgt ggcgatcatt gtgttctcgt tattgagaat tgtgcctttg
     7921 gtaaagacgc tgaagacaga attgctccgt accatcaaca tagttgaaat cgttctcgat
     7981 accctgatgg gtgctttcat gctttatgta gccttatccg gaaaggattt ggcaaacgaa
     8041 ccggtttggg gcggcatcta caaatggtta ttggcaatat tcttttatct gagaggactc
     8101 gtgtttttca attccgtcgt gtttctcggc gagaaaaccg aaattcccaa gttttgggtt
     8161 catatcggct gcctgactct tggtgccata atcgccgtat ggccgaattt tgattacaat
     8221 gtcgtcggag ttttctttct tttgatttcc attaccggag ccgcttacct cggatatgat
     8281 ggatatggcg gttaccgcaa ataccgggaa tattccaaag cgctgaatga aggcaagaag
     8341 gccgcggaaa aaccggcgga agaaaaggaa gttcccgatc cggaaataaa gaaaccggaa
     8401 aaagaaccgg aaaaagttcc tgaaaaagaa ccggaagaaa aagagactta catcaactaa
     8461 aaaacgattg acagccagtc atagataatg tattataaaa tggctgactg ctttccggag
     8521 taatactcaa gaggccgaag aggcgcccct gctaagggcg taggtcgggg aaccggcgcg
     8581 agagttcgaa tctctcttac tccgccagct aaatgaagga aggcggttgg aaacaatcgc
     8641 ctttccatat atgaaaatcc agacataacg gagaagtacc caagtggctg aaggggctgg
     8701 cttggaaagc tagtagatcg ttaacccggt gcaggggttc aaatcccctc ttctccgcca
     8761 tat
//
LOCUS       DAOPJL010000039         8368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_9038_length_8368_cov_8.743414, whole genome shotgun sequence.
ACCESSION   DAOPJL010000039 DAOPJL010000000
VERSION     DAOPJL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9038_length_8368_cov_8.743414"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="PLP02_05210"
     CDS             complement(<1..1514)
                     /locus_tag="PLP02_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42873.1"
                     /translation="MMDLEERYLECLDMENIEEKLDKSNELQNDSLLAKDWDHYVKAT
                     LAVSECQEMLADQESANQTLEEALKHNEMTDYKQIVSLIDSLVRGYLKTEEYEKLLPL
                     LERRENYLEAKRSYKTMQKFYLAVCYEGLGDIKKAIFSLNDVEDTLSGANLASKYLKL
                     SMLYLADGNGELARNALEKAAVFDPKEENPLFHLAKSDLSLFEGDDIKASEHYRKFFV
                     ISKNKNRYLDRFIRIAISQNNLEEAVRFDEKWRDKIDAFYSKHAKKNYYEAVLELYKK
                     LGDFGKAGKLQEELQKMAGEKPPLVDIYEMQKRIFEDMKNIILPSSNRMILLSLCRSL
                     FSSGLYSRLDFVYLKDDGYSVMHYSKGLLLESFLTALETENTLIDKVMSEDDLHQVFQ
                     ETDLGNMKAYRKSEDDEIHEVVIAMRVENESYKKGCFLAYPKSLGIYETANNILKTAV
                     MALEAKIRSNDECLEAKTQEKVYIEALERAGYGMLRIEGDTVKLYDEAVKSILG"
     gene            complement(1511..2032)
                     /locus_tag="PLP02_05215"
     CDS             complement(1511..2032)
                     /locus_tag="PLP02_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPF42874.1"
                     /translation="MTKILVFSDNHRDRSVLKEVLNRETGCDFLFSLGDSEMPEHELS
                     YAGIVGVKGNYPFEPDFPYDLILEFSGVRFMFTHGHRYYVKTGTGILLEKAISEKIDV
                     VFYGHTHEASIEYRNKVLLVNPGALSFSKGKYGKTYLIANVENKKVYVSLREAETGEE
                     VENREITFKKGDE"
     gene            complement(2025..2609)
                     /locus_tag="PLP02_05220"
     CDS             complement(2025..2609)
                     /locus_tag="PLP02_05220"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HPF42875.1"
                     /translation="MKSIMIATKNKGKEKEYRELLAPLGYEVRTLFDFPDIPDIEETG
                     QTFLENAMIKAKQVAKICQIPVLADDSGLEVEALNNRPGVYSRRYAGTGNDLDNNQKL
                     LKEMEGKTNRKARFVCQIVYMEPDKPIRSYEGVAEGTLAYELHPGNGFGYDPLFYVFE
                     AGKYMSQLATEEKNKISHRGKALAKLVKDLADND"
     gene            complement(2698..3363)
                     /locus_tag="PLP02_05225"
     CDS             complement(2698..3363)
                     /locus_tag="PLP02_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPF42876.1"
                     /translation="MNKRTFAVIGVGRFGMALIEELASLKADVLVIDKNYDKIEKVAK
                     IVTNAICLDSTDIEALKEIGIANYDTVIVATGVNVDNSILTTLILKDLGISDIYVKVQ
                     SEYHAKVVEKLGVDEDHLIWPEQAMGKRLAKILVSGNFLDFMELDDDYSFVSFEATKK
                     VIGKHLLELEIRSRFGVNIVAVRRNEKIVIPSSTTPFEEGDEILMVGQNDNIQKFSGW
                     LNK"
     gene            3534..4640
                     /locus_tag="PLP02_05230"
     CDS             3534..4640
                     /locus_tag="PLP02_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPF42877.1"
                     /translation="MNEYDDLFKKPEQIRETSQKPFHSLDPNEEMQKNISEQKINRII
                     FISYFIIAILMSIVSYSFFYFKYPDAATINSNLSIASAPAMTYAESGDEIYPYNITLT
                     GSIANGNDFELPCIFIIYNIYGTDNNLIASQYINQEKVSAGGVFTYEETISSEFEPSY
                     FAYSYGFDESSYFYIIIFLLQLTLSAILFLYIDKVAFVSDFKRFFSNFWNSSGLVVGG
                     IFALITMMYVSQIILSNFFNVYTTSENEMTINSMFAPDPFRLFLLFMILCVLTPIVEE
                     LTFRKALYGFFYRRLGPVVAIIASSLVFGLLHVASYGDFIQAIVYVPMGAVLGIFYYL
                     SKRNVHVTIGIHFINNFISFLIYAFAAYGVTLFA"
     gene            complement(4704..5009)
                     /locus_tag="PLP02_05235"
     CDS             complement(4704..5009)
                     /locus_tag="PLP02_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPF42878.1"
                     /translation="MKSTKIRRNRIMNKSELVARIAELSDLTKKDAENALNSTVVAIQ
                     EALVKGEKVILTGFGTFEVKVRKARSGHDPRTGELINIPPLKAPTFKAGKVLKDSVR"
     gene            complement(5079..6386)
                     /gene="der"
                     /locus_tag="PLP02_05240"
     CDS             complement(5079..6386)
                     /gene="der"
                     /locus_tag="PLP02_05240"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPF42879.1"
                     /translation="MAHTVAIVGRPNVGKSTIFNRIVGQRMAITDDASGATRDRIYAK
                     ASWFGHTFSVIDTGGIEKSDAPFLSLIKSQAEVAIMEADVIIFVVDGKTGPVDDDVTV
                     MKILFKSAKPIIVAVNKADNEAVRSSYYDFYSLGATDIIPVSGIHGTGFGDLMEKVIE
                     YFPEKTEEDYPEEYCKIAVIGRPNVGKSQLTNAILGYDRVIVSDQMGTTTDSIDTTFE
                     RDEKKYVIIDTAGLRKRGKIYEGIEKYSALRAMEAIERADVCLITIDASEGILEQDTH
                     IAGYALENGKAMVIVVNKTDLMEDKQRFMAEWRQKIAVAFSFMTFVPTIFLSAKTKSG
                     ISEIFPKIDTVYSNYSRRVQTSVLNEVLSEAIISNPPKEYNHRIVKVYYATQAKTKCP
                     AFILFVNSTDCMHFSYRRYLENRLRERFDFSGTPIKLVLRNRD"
     gene            complement(6408..7811)
                     /locus_tag="PLP02_05245"
     CDS             complement(6408..7811)
                     /locus_tag="PLP02_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPF42880.1"
                     /translation="MENNLENEGKIFIPKVGNILTGTVVKVTDDEVLVDVGFMCEGTI
                     YKEYLSKEKIKSAKDLFKVGDEITAKVTKISVGDESSVLLLSRLDLEKQAAMDKYRQD
                     LEIGKNVVAKVKKAVKGGLLLDYNTIELFLPDSLLSLENTNEEAKKALVKSEIEVRVI
                     DIRNDRNREKFIVNRKQVLYDNLKKRERDEIGDFEVGQIVKGKVTRIMEYGAFVKIGE
                     YAEGLVHISEISHSRTKKVEDVLKEGDEVEAKIIKISGKRVSLSIKATQKTPWDTFME
                     NHKLGDKVTGKVIKKMQYGMLLEIDKDTKGLLNRIDYSWDPNDNLAGRLEVGDSIEVE
                     ITGINEEKQQFTLSRKHLEYNPWADLKLKVGEQVSATVKTILERGAVLEVSGVEAFLP
                     IREISSDHINRVEDVLKVGDILTVEVTSFYPREWKMSVSLRKLNEKNQRSEYESHLKE
                     NVSANQSLAELFEKYKK"
     gene            complement(7811..>8368)
                     /gene="cmk"
                     /locus_tag="PLP02_05250"
     CDS             complement(7811..>8368)
                     /gene="cmk"
                     /locus_tag="PLP02_05250"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPF42881.1"
                     /translation="ATTLKALDMGIDIEDADKFGFLDDTLMEFKGDELYVDGKNVTRE
                     IRSNRVSNNVSVVSSHIPVRNRLVKIQQDIATRDNVVMDGRDIGTVVLPNADLKIYMT
                     ATVAERARRRHEENLANNMPSDYETLKKEIEWRDAYDSTRSYNPLRQAEDAVYLDTSE
                     KDINQVANLIYDMFNEAINRQEEQK"
BASE COUNT         1978 a         1996 c         1673 g         2721 t
ORIGIN      
        1 tgtcctaaaa tacttttgac tgcttcatcg tataacttga ctgtgtcacc ttcgattctc
       61 agcattccgt atccggctct ttcgagagct tcaatatata ctttttcttg ggtttttgct
      121 tcaaggcatt cgtcgtttga cctgatcttg gcttcaagag ccatgaccgc tgttttcaga
      181 atgttgttcg cggtttcgta aatacccaag gatttcggat atgcaagaaa acatcccttt
      241 ttgtaagatt cgttttccac tcgcatggca atgaccactt catgtatttc atcatcctca
      301 ctttttcggt aagccttcat gtttcccagg tcggtttcct gaaagacctg atgcagatca
      361 tcttccgaca tcactttgtc tatcagcgta ttttcggttt ctaaagctgt cagaaaagat
      421 tccagaagca atcctttgga ataatgcatg acagagtatc cgtcatcttt taaataaaca
      481 aaatccaagc gactgtaaag tccggaagaa aatagcgaac ggcataggga aagaagaatc
      541 atccggttac tagaaggaag aatgatgttc ttcatatctt cgaatattcg cttctgcatt
      601 tcataaatat caacaagcgg cggcttttcc cccgccatct tttgcaattc ttcctgcaat
      661 tttccggctt taccgaaatc tcccagtttt ttgtaaagtt ccaacaccgc ttcgtagtaa
      721 ttctttttgg catgcttact gtaaaaagca tctattttgt cccgccattt ttcatcgaac
      781 cggaccgctt cttctaaatt attctggctg atcgcgattc tgatgaatct gtcaagatac
      841 cggtttttgt ttttggagat tacaaaaaat tttcggtaat gttcagaagc tttgatgtcg
      901 tctccttcaa aaagactcag atcggacttg gcaagatgaa aaagcggatt ttcctccttc
      961 gggtcgaaca cggccgcttt ttccaaggcg tttctggcaa gttcaccatt tccgtccgcc
     1021 agatacagca tcgataactt aagatacttg ctggcgagat tcgccccgga cagagtatcc
     1081 tcgacatcgt tcaaagaaaa aatggctttt tttatatctc ctaacccttc gtaacagacc
     1141 gccagataaa acttttgcat ggtcttgtaa gaacgtttgg cctctaaata gttttccctt
     1201 ctctctaaaa ggggaagcaa tttttcatat tcttcggttt tcaaataacc gcggaccaag
     1261 gaatcgatca gcgatacgat ttgcttgtaa tcggtcatct cgttatgttt caatgcttct
     1321 tccagggtct gattggccga ttcctgatcc gcaagcattt cttgacattc cgaaaccgca
     1381 agggtggctt tgacataatg gtcccagtct tttgccaaaa gcgaatcatt ctggagttcg
     1441 ttcgatttgt ccagtttttc ctcgatgttc tccatatcaa gacattctag atatctttct
     1501 tcaaggtcca tcattcatcc ccctttttga aagtgatttc tctgttttcc acttcttcgc
     1561 cggtttcggc ttctcttaag gatacataaa cttttttgtt ttcaacgttg gctatcaagt
     1621 atgtttttcc atattttcct ttagaaaacg acaaagctcc gggattgacc agaagcactt
     1681 tgtttctgta ttcgatggat gcttcatggg tatgtccgta aaatacgaca tcgatctttt
     1741 ccgatattgc tttttccaat aggataccgg ttcctgtctt tacataatat ctgtggccgt
     1801 gagtgaacat gaatcttaca ccggaaaact ccaggatcag atcgtaaggg aagtccggtt
     1861 caaaaggata attacctttg actccgacaa ttcccgcata agacagttcg tgttcgggca
     1921 tttccgaatc tccgagcgaa aacaggaagt cgcatcccgt ttcccggttc aacacttcct
     1981 ttagcacact tctgtcgcga tggttgtcgc taaacaccaa aatcttagtc attatccgcc
     2041 agatccttca ccagtttggc taaagcctta ccgcgatggc tgatcttgtt cttttcttca
     2101 gtcgcaagct gtgacatata tttgccagct tcaaaaacat aaaacaaggg atcgtatccg
     2161 aatccgttcc cgggatgaag ttcgtatgcc agcgttcctt ccgccacacc ttcgtaacta
     2221 cgaattggtt tatccggttc catataaaca atctgacaga caaaccgggc tttccggttt
     2281 gtttttcctt ccatttcctt taacagcttt tgattgttgt caagatcatt tccggtaccg
     2341 gcataacggc gtgaatagac tcccggccgg ttattcaggg cttcgacttc caagcccgag
     2401 tcatcggcca aaaccgggat ttgacaaatc ttggccacct gtttcgcctt gatcatagcg
     2461 ttttccaaaa aagtttggcc ggtctcttcg atatccggta tatccggaaa atcgaaaaga
     2521 gtccttactt cataaccgag cggcgctaac agctcccgat actctttttc tttgcctttg
     2581 tttttggttg cgatcatgat cgatttcatt ttttcacctg taaaaagtat atcacatacg
     2641 actactcaga cgcttcgcaa atatgaaaaa aagcgggaaa tagtttcccg ccccgtttca
     2701 cttgttcagc catccggaaa atttctggat attgtcgttt tggcctacca tcaggatttc
     2761 gtcgccttct tcgaaaggcg ttgtcgaact gggaatgacg attttctcat tccttctcac
     2821 cgccacgata tttactccga agcgtgatct gatttccaat tcgagaagat gttttccaat
     2881 gacctttttg gtggcctcga acgaaacgaa actgtagtcg tcgtcaagtt ccataaaatc
     2941 aaggaaattg ccggagacga gaatcttggc cagacgtttt cccatggcct gttccggcca
     3001 gatcaaatgg tcttcgtcca cgccgagttt ttcgacgact ttggcgtgat attcgctttg
     3061 aactttcacg tatatgtcgc ttattcctag atccttcagt atcagagtcg tcagaatcga
     3121 attgtccacg ttgactcccg ttgcgacaat taccgtatcg taattcgcga ttccgatttc
     3181 cttaagcgcc tcaatgtcgg ttgaatccag gcaaatggcg tttgtgacta tcttggcgac
     3241 tttttcgatc ttgtcgtagt ttttgtcgat taccagaaca tcggctttta aacttgccaa
     3301 ttcttcgatc agagccattc caaaacgtcc taccccgatc accgcaaatg tccttttgtt
     3361 cattgtaaga cttcctttcc tttgaagcca taagtatatt atacacaaaa aaggcttttt
     3421 gtcaattgtg tgagaattac cacatttcct tgcgataact catgtttttc tttttttaat
     3481 cgtgaaaata cggtataatg aatataatta atttttgaca aaggagtaag ccgatgaacg
     3541 aatacgatga cctattcaaa aagccggaac aaatccgtga aaccagccaa aaaccgtttc
     3601 attccttaga tccgaacgaa gaaatgcaaa aaaacatatc cgagcaaaag atcaaccgga
     3661 taatattcat ttcctatttc atcatagcga ttttgatgtc gatcgtctca tattcatttt
     3721 tctatttcaa atatccggat gccgccacaa tcaattccaa tctttctata gcgtcggctc
     3781 cggccatgac ttatgccgaa tcgggagatg agatctatcc ttacaacata acgttgacgg
     3841 gatccatcgc caacggcaac gatttcgaac ttccttgcat ttttatcatc tataacatat
     3901 atggtaccga taacaatctg atcgcatccc aatacataaa ccaggaaaag gtctccgctg
     3961 gcggtgtctt cacttacgaa gaaactattt ccagcgaatt cgaaccttca tattttgctt
     4021 attcttacgg ttttgatgaa agttcatatt tctacatcat cattttcctt ttacaactga
     4081 cgctttccgc aattttgttc ctttatatcg acaaagtcgc cttcgtctct gactttaaac
     4141 gcttcttctc caatttctgg aattcttccg gattggtagt gggaggcata ttcgctctca
     4201 tcactatgat gtatgtttcg cagataatcc tatctaattt tttcaatgtc tatacaactt
     4261 cggaaaacga aatgacgata aactcgatgt tcgctcccga tcctttccgg ttatttttgc
     4321 ttttcatgat cctttgcgtg cttactccga tagtggagga actgactttc cggaaagccc
     4381 tttacggttt cttttaccgt cgtttgggcc cggttgtggc gatcattgcc agcagcttgg
     4441 tattcgggtt gttgcatgtc gcttcctacg gggatttcat ccaggcaatc gtttatgttc
     4501 ccatgggagc cgtcctcggt attttctatt acctttccaa acgaaatgtc catgtgacga
     4561 tcggcattca tttcatcaac aatttcatct cttttctgat ctatgccttt gcggcctacg
     4621 gcgtaacgct gtttgcctga aaacaatcca ctataaacgt aaaaaaagcg tctcgctcgg
     4681 agacgctttc tttttgaatt tgattatctt acggaatcct tcaggacttt tccggctttg
     4741 aatgtcggtg ctttcaatgg cggaatgttg atgagttcgc cggttctggg gtcatgaccg
     4801 gaacgggctt tgcggacttt aacttcaaat gttccaaaac ctgtgagtat gactttttct
     4861 cctttgacca aagcttcctg aatggcaacc actgtagagt tcaaggcatt ttctgcatct
     4921 ttctttgtta ggtcggataa ttcggcaatt cgtgcaacca attcggattt gttcataatt
     4981 ctattcctcc taatttttgt tgatttcatt ataatgggtt tatttttaaa ttgcaagcag
     5041 tttaccgaaa atatcccctt tttcgggcta aatccgtgtc aatcacgatt tctcaaaacc
     5101 aattttatcg gtgtaccgga gaaatcgaaa cgttcgcgga gccggttttc caagtatctt
     5161 ctgtaactga aatgcataca atcggtactg tttacgaaaa ggatgaaggc cggacatttc
     5221 gtcttggcct gagtggcgta atagaccttc acgatccggt gattgtattc cttcggagga
     5281 ttggaaatga tcgcttctga caacacctca ttcaatactg atgtctgtac ccgacgggaa
     5341 taatttgaat acacagtatc gattttcggg aatatctcac tgattccaga cttggttttt
     5401 gcagatagga aaatagtcgg aacgaaagtc atgaaagaaa acgcgacagc gattttttgt
     5461 ctccattcgg ccatgaatcg ttgtttgtcc tccatgagat cggtcttgtt tacgacgatc
     5521 accatggctt tcccgttttc cagagcatat ccagcgatat gggtatcctg ttcgagaatg
     5581 ccttcagaag catcgatcgt tatcaggcaa acatccgccc tttcgatcgc ttccatcgct
     5641 ctcaaggctg agtatttctc gattccttca tatatcttgc cgcgttttcg caaaccggcc
     5701 gtatcgataa tcacatattt cttttcgtct ctttcgaaag tcgtgtcgat tgaatcggtg
     5761 gtggttccca tctgatctga cacaatgacc cggtcataac cgagaatagc gttcgtaagc
     5821 tgagattttc cgacattcgg tctgccgata acggctatct tgcaatattc ttccggataa
     5881 tcttcttcgg ttttttcggg aaaatactcg ataacttttt ccatcagatc gccaaagccg
     5941 gttccatgga ttccggacac cggtatgata tcggtggctc ccaaagaata aaaatcgtaa
     6001 taactgcttc tgaccgcttc gttgtccgct ttgttgacgg ccactattat gggtttagcg
     6061 gacttgaaaa ggattttcat caccgtcacg tcgtcatcaa ctggaccggt ttttccgtca
     6121 acaacgaaaa ttatgacatc ggcttccatg atggccactt ccgcctgtga ttttatcaaa
     6181 gacaaaaaag gtgcatcgct tttctcgatg ccccccgtgt cgatcacgct aaatgtgtgt
     6241 ccgaaccagc tggctttggc gtaaatccgg tcccgggtgg ccccggatgc atcatcggta
     6301 atcgccatgc gttggccgac gatccggttg aagatcgtcg actttccgac attagggcgt
     6361 ccgacgatgg cgactgtgtg cgccatggcc atcactcctt ttttggctta ctttttgtat
     6421 ttttcaaaca attccgccaa cgactggttg gctgagacat tttctttgag atgcgattca
     6481 tattcgcttc tttggttctt ttcgtttaat ttgcgaagcg aaacggacat tttccattcc
     6541 ctcggataga aggaagtcac ttcgacagtc agtatgtcgc cgactttgag aacgtcttct
     6601 acgcggttga tgtgatcgga agatatttca cgaatcggaa gaaatgcctc cactccggat
     6661 acttcgagga cggcgcctct ttccagaatt gtcttgactg tggccgaaac ctgttctccg
     6721 actttcagtt taagatcagc ccacggattg tattccaagt gttttcttga caatgtgaac
     6781 tgttgctttt cctcgttgat tccggtgatt tcgacttcga ttgagtctcc gacctcgaga
     6841 cgcccagcca ggttgtcatt gggatcccag gaataatcta tcctgttcaa aaggcctttt
     6901 gtatctttgt cgatctcaag aagcattccg tattgcatct tcttgatgac ctttcccgtg
     6961 accttgtcgc ctaacttatg gttttccata aaagtatccc atggcgtctt ttgtgtggct
     7021 ttgatagaga gggaaacgcg ttttccggag atcttgatga tcttggcttc gacttcgtcg
     7081 ccttctttta aaacatcttc gactttcttt gtgcgggaat gcgaaatctc gctgatgtgg
     7141 accaagcctt cggcgtattc gccgattttg acaaaagcgc cgtattccat aatcctggtt
     7201 acttttccct tgacaatttg tccgacttcg aaatcgccga tttcgtcgcg ttctcttttt
     7261 ttcaggttat catagagaac ctgtttgcgg ttgacgatga atttttcccg attgcgatcg
     7321 tttctgatgt cgataacgcg aacctctatt tcgcttttga caagcgcttt tttcgcttct
     7381 tcgttggtat tctcaagcga caataacgaa tccggcagga acagttcgat ggtattataa
     7441 tccagcaaca acccgccttt gaccgctttc ttgactttgg cgacgacatt tttacctatt
     7501 tccaaatcct gacggtattt gtccatagcc gcttgttttt ccagatcgag acgcgataaa
     7561 aggagaacac tcgattcgtc tcccacgctg attttggtta cttttgcggt tatctcgtct
     7621 cccactttga aaagatcctt ggcacttttg atcttctctt tcgaaagata ttctttgtat
     7681 attgtccctt cgcacatgaa accgacgtcg accagaactt cgtcatccgt cactttcacc
     7741 actgtgccgg taaggatgtt tccaaccttg ggtatgaata ttttgccttc gttttcgaga
     7801 ttgttttcca ttatttttgc tcctcttgtc tgtttatggc ttcgttgaac atatcataaa
     7861 tcaggttggc aacctgattg atgtcttttt ccgatgtgtc gagatacacg gcgtcttcgg
     7921 cctgccgcaa aggattgtag ctccttgtgg aatcataggc atcgcgccat tcgatctctt
     7981 ttttcagggt ttcgtagtcc gatggcatgt tgttggccaa attctcctca tgccttcttc
     8041 tcgccctttc ggcgactgtc gccgtcatat agatcttgag atcggcattg ggaagaacca
     8101 cggtgccgat gtcgcgtccg tccatcacaa cattgtccct ggtggcgatg tcctgttgga
     8161 ttttcaccag tctgttgcga acggggatgt gacttgaaac gaccgaaacg ttgtttgaaa
     8221 cccggtttga cctgatttcc ctcgtgacgt tcttgccgtc gacatagagt tcgtctccct
     8281 tgaattccat caaggtatcg tcgaggaaac cgaatttatc ggcatcttcg atgtctattc
     8341 ccatgtcaag tgctttcagc gttgtcgc
//
LOCUS       DAOPJL010000040         8192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_9309_length_8192_cov_8.778051, whole genome shotgun sequence.
ACCESSION   DAOPJL010000040 DAOPJL010000000
VERSION     DAOPJL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9309_length_8192_cov_8.778051"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLP02_05255"
     CDS             <1..265
                     /locus_tag="PLP02_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42882.1"
                     /translation="VQSSRGTNPGEPEIETTPFEAEYVVVQKQFSAFAVGEQVKITVL
                     IWYEGEDADHNNDIIGGGVKFEMVFHITYIYEDNEIPNTTIEG"
     gene            313..1287
                     /locus_tag="PLP02_05260"
     CDS             313..1287
                     /locus_tag="PLP02_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42883.1"
                     /translation="MKSLKRKVLLSAIVLMFALVATVGSTYAWFTVSNTVTVQSMTLN
                     VQSVDNLLIRMEDPGTADASEPNLDQPSTYFSSLTTGQIMTYYNYASWKLYPVTAIQT
                     GYATTDAKALNTMEVSSSNYLRALTATVAGDKNSATGKYVELKFWLYLQTNDGLTRNI
                     ALQDLTITSSYGTTAQQAVVNAIRLGVWGDNTTYGTGGSAGSAYIFGIGNDYDFAFTS
                     AMPGYDASATGSGYIPGTNTFNYMDQLDAAYTLSTAAQSDDLFYSASPIANMSTDTLT
                     SAQTIFAMNPAEATLFTVRIYVEGWDAQALNDLSGAQFNISFKFTIQA"
     gene            1535..2224
                     /locus_tag="PLP02_05265"
     CDS             1535..2224
                     /locus_tag="PLP02_05265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPF42884.1"
                     /translation="MEVTKKGTRAWKIVGNVVFWVLIVVVVFYSVVALFSKKEENMTS
                     FFGLTALTVKSDSMKTTFNKGDLIFITTDFDSENLNPGDVVTYLVTTTVNGETETYYN
                     THRISRIDGVFIYTQGDNPELSEDPYYITSNDVIGMYTGVHLLGMGTFIDFLKSSTGF
                     FLFIVLPCLAFLVYEIFRFVKVYSQYQVQKSMNDRVQMQEEALAAARAQLEQEQKEKA
                     DKKVPGENDKE"
     gene            2274..3917
                     /locus_tag="PLP02_05270"
     CDS             2274..3917
                     /locus_tag="PLP02_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42885.1"
                     /translation="MVEFSRYYIQLFKDIWDNIVELFQYGIWENFLKRIGLDFYEYYK
                     DLYDYSQSFTIVSWIMAIISLILNFSLIIFIIIKLVIWFRKYAAFRKIEVEKNELIEE
                     IALLNDKVIALMDEKNQIMAMKVSQLGINASTGEPAEKIITGPLNRGQKMQPAMEYAG
                     NGNGLVRSPQSVIMPERSQSDLPPGMSRFVKLIQVDKEYAGKDTHIYMEEGDYLQLSE
                     LVDKFVDFASSKLKLYYTREMIAQFFSGMAASKVMILEGISGTGKTSLPYAMGKFFGN
                     NASIISVQPSWRDRSEMLGYLNEFTKRFNETEFLKALYEASYRKDVDFIVLDEMNLAR
                     IEYYFAEFLSIMEMPDPSEWKIDLVPDSKSSDPLKLINGKITVPLNVWFVGTSNKDDS
                     TFTITDKVYDRAIALEFEAKGEYFPAPETEPIRMSSEYLQELFDEAFYKYPVSEGLLD
                     KFEILDKYIQSNFKIAFGNRIMVQVHKFVPVYVACGGTEEKGLDYIFANKILRKFETL
                     NLAFLQNELDQLINQIHRVFGKNGFEESIKCIQDLKKHI"
     gene            3930..5480
                     /locus_tag="PLP02_05275"
     CDS             3930..5480
                     /locus_tag="PLP02_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42886.1"
                     /translation="MEDSEVKKYFKKINTTFAGVNKQDDFARMFLNLIKSGNTTLYQK
                     ERRERRIFDDSWMDSVEGAIPVIDKLTRNPREILKKVNLVVPVERAKHIDQDTVKHLA
                     QNTHLIKDVDEEGNVIPSKVLTYFNESDLGTYENRFLRSFVDKLYTFIEKRYDLVVQK
                     MHTEYVNYLNIKSEVEWEESTIEFDITMRLNEIIKKDEIDMKNQELFDRMTKIRRNIT
                     IFKMSDFMQEMKKFSPVTPPIMKTNLIMKNQDFRQCYYLWVLMDQVDRIGYDIDIFER
                     DVEFDEGYMSGIENMLMVLYSTVANNQNDEFVIAQNEPYEYRKIKRPRIKKTDQNDQY
                     VQPGYYEFEDNSLNQYYLDQVRSANQSRFKSLEDAGIPMTESIDIVFQQMSQITNAIY
                     EDFIRHSFRPEEEKDLEQRIRLQEQILEVYRQVEKIKRDDMRQLSTTKAIALLNLRNY
                     QDELKARKKFEQEEKERAEEEEKMSDDIETKAKAESEADKKRQLERARKILEDARKER
                     EKKQTDES"
     gene            5532..6146
                     /locus_tag="PLP02_05280"
     CDS             5532..6146
                     /locus_tag="PLP02_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42887.1"
                     /translation="MKQRIVLSLIAMMGSVFLLIFASFAWFAVSNSNNVDGTDVDVVN
                     LDVEVTLEISMDGVTYVPADNIAIDGAVPGDLVYYRISLDNTGNVPVNVRISLSGFTD
                     QAADANFSYDDTKTLRDVTLVNCSNTVNSETITGQLIADLLPYVPSGDYSNQSFDLAS
                     SITLPVSGIGIVYFTLQVSGNAGNDYLNQSIQIARLSIESAMSS"
     gene            6159..6791
                     /locus_tag="PLP02_05285"
     CDS             6159..6791
                     /locus_tag="PLP02_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42888.1"
                     /translation="MKTNIRAIVASILFMVFAALVVVGFTYGWFSFGTIYTTNHVNVG
                     NLEYTLSGAFLNDNSVVVPGEDLVVDNFSINNESSVSSQLRIKITYTRVTDVGGTPTP
                     EQGYVYKGDSDDHIAVIFMSTFLFTDGDATPDEYDDDYWYYGDYATEINAESGVIDLI
                     SNIHYDGDSTSTEYNGQTIAVTVLIEVKQADNVTWSELATYNFETGYPIA"
     gene            6828..7508
                     /locus_tag="PLP02_05290"
     CDS             6828..7508
                     /locus_tag="PLP02_05290"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPF42889.1"
                     /translation="MKKKKKIGTFLLQFLPLILIIFSLVLIGKSILSLQSGKTPTIFK
                     YAVLYIEPDTDSMVDVIMPSDIIFVDTRAHDYQEGDIISFWAKDRVGGTLVTLCVTHE
                     IIAIDTTSGEKVYITQGVNNPSPDSWADGWEITDDKIIGLYVGRSAFLGNVYKLVAAN
                     KLGVAYAGVIIVFLLIIISEIANITKEVALGKQKQATMDKEKMIQEELEKLKSEHEDR
                     KDNGPLAK"
     gene            7635..>8192
                     /locus_tag="PLP02_05295"
     CDS             7635..>8192
                     /locus_tag="PLP02_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF42890.1"
                     /translation="MEIYVLASGSGGNATYVKIGNTNILIDAGISLRQITSRLSQLDK
                     TLDGIDHIFLTHEHGDHIAGLLTIQKKYGSVVHMTMGTYKNLPIRISDNLDMRKVNYL
                     NYLEPVVLDGFSVLPFMTYHDALEPCGYRFTESGKSLVYMTDTGYYPRTGYDWIRNAD
                     IVVIESNHEPEMLLESERPWLTKKRI"
BASE COUNT         2452 a         1808 c         1827 g         2105 t
ORIGIN      
        1 agtccagagt tcaagaggaa ctaatccggg agaaccggaa atagagacca caccgttcga
       61 agcagaatat gtggtggtcc aaaaacaatt ttccgctttc gcagtcggag aacaggtgaa
      121 aatcaccgtt ctgatctggt acgaaggcga agacgccgac cataacaacg acatcatcgg
      181 tggaggcgtg aaattcgaga tggtattcca cattacctat atctatgagg ataatgagat
      241 tcctaatacc acaattgaag gctgagattt ctgaaattca aataaataaa aaattcataa
      301 aggagaagaa taatgaaatc gttaaaaaga aaggtattac tttccgcaat cgtattaatg
      361 ttcgcgttag tcgcgacagt cggatcgact tacgcatggt tcacggtatc aaatactgtt
      421 actgtacagt caatgactct gaatgttcaa tcagtggata acttattgat tagaatggaa
      481 gatccaggga cagctgatgc atccgaacct aatttggatc aaccgtcgac ttatttctct
      541 tcattgacta ctggacagat catgacgtat tacaattacg catcatggaa attgtatccg
      601 gttactgcaa ttcagactgg atatgcaaca actgatgcta aagcattgaa cacaatggaa
      661 gtcagttcaa gcaactattt gagagctttg acagccactg tggcaggaga caagaactct
      721 gccactggaa aatatgttga actcaaattc tggctctatt tgcaaaccaa tgacggattg
      781 acaagaaata tcgctttgca ggatttgacc attacatcat cttacggcac caccgctcag
      841 caagctgttg tcaacgctat cagacttggt gtctggggcg acaacacgac ttatggaact
      901 ggtggcagcg caggcagcgc ttatattttc ggtattggaa acgattatga ttttgccttt
      961 acttccgcta tgccgggata tgatgccagc gcaactggca gcggatatat tcctggaaca
     1021 aacacattca actacatgga ccagcttgat gcagcataca cactttcaac agcggctcaa
     1081 tctgatgatt tgttctattc ggcttcaccg atagccaaca tgagcacgga tactttgaca
     1141 agtgcccaaa cgattttcgc tatgaatccg gcagaagcaa ctttgtttac agtacgtatc
     1201 tatgttgaag gctgggatgc ccaagctttg aatgacctga gtggagctca gttcaacatt
     1261 tccttcaaat tcacaatcca agcataattt attatcaatt aaatattagc gcagccagag
     1321 attattctcg ggctgcgccc cctccggggg gtgctttttt caaggttatg caatctttgc
     1381 gtatctttga aaaaagtaag ataattataa aaagaacccg atttatggct ttttctcgga
     1441 catattatga aaaatgtcct gtattattcc aaatttttac gttataatat aagttagcgt
     1501 tattctcatg acttatattt ggggagtgaa ataaatggaa gttacaaaga aagggacaag
     1561 agcttggaaa atcgtcggga acgtcgtttt ttgggtttta atagtcgttg tcgtctttta
     1621 ttcggtagtt gccctattct caaagaaaga agagaacatg acttctttct tcggattgac
     1681 cgcgttgacc gtgaagtccg attctatgaa aacaacattt aacaagggag atttgatttt
     1741 tattacaacg gattttgatt cagaaaatct aaaccccggc gatgttgtca cgtatctggt
     1801 tacaacgact gtaaatggtg aaacagaaac atattataac acccatagaa tctcgcgcat
     1861 tgatggagta ttcatttata cgcaaggtga taatccggaa ttatctgagg atccatatta
     1921 catcacttca aatgatgtga tcggaatgta taccggagta catcttttag gaatggggac
     1981 attcatcgat ttcttgaaat cgtctacggg attcttcctg ttcatcgtcc tgccatgctt
     2041 ggctttcctt gtgtatgaga tattccgttt tgtcaaagtt tattcccaat accaggtcca
     2101 gaaatcaatg aatgaccggg tgcaaatgca agaagaagcg ctggctgccg ccagagccca
     2161 gttggaacag gaacagaagg aaaaagcgga taaaaaagta cccggagaga acgacaagga
     2221 ataaaatccg attccgcata actgttgcca ttccggagag gaggagttga catatggtcg
     2281 aattttccag atattacatt caactcttta aggatatttg ggacaatatc gtcgaactct
     2341 ttcagtatgg catctgggaa aattttctaa agcgtatcgg tctggatttt tatgagtatt
     2401 acaaggattt atacgattat tcccaaagtt tcacgattgt atcgtggatc atggcaataa
     2461 tcagtctgat acttaatttc tcgcttatca ttttcatcat cataaaattg gtcatttggt
     2521 tccgtaaata tgccgctttc cgcaaaatcg aagtggaaaa gaacgagctt atcgaggaaa
     2581 tcgctttgct taatgacaaa gtgatcgccc taatggacga aaagaaccag attatggcca
     2641 tgaaagtctc gcaattagga atcaacgctt ccaccggaga accggcggaa aagatcatca
     2701 caggcccgtt aaaccgcggc cagaagatgc aaccggccat ggaatatgcc ggcaacggaa
     2761 acggacttgt ccgttcaccg caatccgtca tcatgccgga aagatcccaa agcgatctgc
     2821 cgccagggat gtcccgtttt gtaaaactga tccaggtcga caaggaatac gccggcaagg
     2881 atactcatat ttatatggaa gagggagatt atctccagtt gtccgagctt gtggacaaat
     2941 tcgtcgattt tgcttcctcc aaactcaaac tgtactacac acgtgaaatg atagcccagt
     3001 tcttttccgg catggccgct tccaaagtca tgatccttga aggtatatcc ggaaccggaa
     3061 aaacttcgtt accatacgca atgggtaagt tttttggtaa caacgcttcg atcatttcgg
     3121 tccagccttc atggcgtgac cgctccgaaa tgcttggcta tctgaatgaa ttcaccaaac
     3181 gtttcaacga aaccgaattc ttgaaagcct tgtacgaagc ttcatacagg aaagacgttg
     3241 atttcatcgt tcttgatgaa atgaaccttg cccgtatcga atattatttt gccgaattcc
     3301 tatcaatcat ggaaatgccc gatccttccg aatggaagat cgatttggtt cccgattcga
     3361 aatcgtccga tccgttaaaa ctcataaacg gtaaaatcac tgttccgctc aacgtatggt
     3421 ttgtcggtac gtccaacaag gacgactcga ctttcacgat caccgacaaa gtctatgacc
     3481 gtgccatcgc tcttgaattc gaagccaaag gggaatactt cccggctccg gaaacggaac
     3541 cgatccggat gtcgagcgaa tacttgcagg aactgtttga cgaagcattt tacaaatatc
     3601 cggtatccga gggacttctt gacaagttcg aaatcttgga caaatatatc caatccaatt
     3661 tcaaaattgc ttttggaaac cggatcatgg ttcaagtcca taaatttgtt ccggtctacg
     3721 tcgcttgcgg cggaaccgaa gaaaagggac ttgattacat attcgccaac aagatcctgc
     3781 gcaagtttga aaccttgaat ctagcattct tgcaaaacga gctcgatcag ctgatcaacc
     3841 agatccaccg tgtattcgga aagaacggct tcgaagaatc catcaagtgc atccaagacc
     3901 tgaagaaaca tatctaggga ggttcccaga tggaagacag tgaagtcaaa aaatatttta
     3961 agaagataaa cacgacattc gctggtgtca acaagcaaga tgatttcgcc agaatgtttt
     4021 tgaatctgat caagagcggg aatacgaccc tataccaaaa agaacgtcgt gaacgccgga
     4081 tattcgacga ctcatggatg gattcggttg agggagcgat tcccgttatt gataaactga
     4141 ccaggaatcc ccgggaaatc ctgaaaaagg taaacctggt cgttccggtc gaacgagcaa
     4201 agcacatcga ccaggacaca gtcaaacatc ttgcccagaa cactcatctc atcaaggatg
     4261 tcgacgaaga aggaaacgtc ataccgagca aagtcctgac ctatttcaac gaatcggacc
     4321 tcgggaccta cgaaaaccgc tttctgagat ctttcgtgga taaattgtac acattcatcg
     4381 agaaacgcta cgatctcgtc gtgcagaaaa tgcataccga gtacgtcaac tatctcaata
     4441 tcaaatccga agtcgaatgg gaagaatcga ccatcgaatt cgacatcacg atgcggctta
     4501 acgaaatcat caaaaaagat gagatcgaca tgaagaatca ggaactgttc gaccggatga
     4561 cgaagataag aagaaacatc actatcttca aaatgtcgga tttcatgcag gaaatgaaga
     4621 aattctcgcc ggtgaccccg ccgatcatga agaccaatct gatcatgaaa aatcaagatt
     4681 tccggcagtg ttactatctt tgggtcctga tggaccaggt cgaccgtatc ggctacgata
     4741 tcgatatatt cgaacgggat gtcgaattcg acgaaggcta tatgagcggc atcgagaaca
     4801 tgctcatggt gctttattcg accgttgcga acaaccagaa cgacgaattc gtgatcgccc
     4861 agaacgaacc ttacgaatat cgcaagataa aacgtccgcg gatcaagaaa accgatcaga
     4921 acgaccagta tgtccagccg ggttattatg aattcgagga caattctttg aatcagtatt
     4981 atctggatca agtccgttcc gccaaccaat cccggttcaa atcgttggaa gatgccggta
     5041 ttcccatgac ggaatcgatc gacatcgtct tccaacaaat gagccagata accaatgcga
     5101 tttacgagga cttcatccgg cattcattcc gtccggaaga agagaaagat ctcgaacaaa
     5161 ggatcagact gcaagaacag attcttgaag tttaccggca agtagaaaaa atcaagcgcg
     5221 acgacatgcg ccaactgtcc acaaccaaag cgatcgctct gctcaatctg agaaattatc
     5281 aggatgagct aaaagccagg aagaaattcg aacaagagga aaaagagcgg gccgaagaag
     5341 aggaaaaaat gtcggacgac atcgagacaa aagccaaagc cgaaagcgag gcagacaaaa
     5401 aacgtcagct ggaaagagcg agaaagattc tggaagatgc cagaaaggaa cgcgaaaaga
     5461 agcagacgga cgaatcctga taattccatt ttgaatatat gaaatattaa taaatgcgag
     5521 gtgaaaataa agtgaaacaa cgcatcgtct tgtcgcttat cgccatgatg gggtccgttt
     5581 ttttactgat tttcgcctct tttgcatggt ttgccgtttc taattccaac aatgtggacg
     5641 gaaccgatgt cgatgttgtc aatttggacg tcgaagtcac tctggaaatt tcaatggacg
     5701 gagtgacgta cgtaccggcc gataacatag cgattgacgg cgccgttccc ggcgatctcg
     5761 tctattaccg tatttcgctt gacaataccg gaaacgttcc cgtcaatgtg agaatatcgc
     5821 tttctggttt caccgatcag gccgccgacg ccaatttttc ttatgatgat accaagacac
     5881 tccgggacgt gacgctagta aactgttcca acacagtgaa ttccgaaacg ataaccgggc
     5941 aattgattgc ggacttgttg ccatatgtgc catccgggga ttattccaat caatcgttcg
     6001 atctggcatc cagcataact ttgccggttt ccggaatcgg aatcgtctat ttcacccttc
     6061 aggtttccgg caatgccgga aacgactatt tgaatcaaag catacagatc gcgcggcttt
     6121 ccatcgagtc cgccatgtct tcctgaggag cgtcggttat gaaaacaaat atccgggcga
     6181 tagtcgcctc catcctgttt atggttttcg ccgctctagt tgtcgtcgga tttacttacg
     6241 gctggttttc tttcggtacc atatatacga cgaaccatgt caatgtcggt aatctggaat
     6301 acacgctttc cggagctttt cttaatgaca attccgttgt cgtgccggga gaagatctgg
     6361 tggttgataa tttctcgata aacaatgaat cgtcagtttc atcccagtta cggatcaaaa
     6421 taacctatac gagagtaacc gatgtcggcg gaacgccgac gccggaacag ggatatgttt
     6481 acaaagggga ttcagacgac cacattgccg taatctttat gtccactttc ttattcacgg
     6541 acggtgatgc gactcccgac gaatatgacg acgattactg gtattacggt gattatgcga
     6601 ccgagataaa cgcggaatcc ggagtgatcg atttgatttc caacatccat tacgacggcg
     6661 attccacaag tacagagtat aatggacaaa ccattgcggt gacggtgctg atcgaagtta
     6721 aacaagccga caacgtcacg tggtccgaac tggcgaccta caatttcgaa accggatacc
     6781 cgatcgcctg attagaataa aaaacatggg aacggagggg tgaagacatg aaaaaaaaga
     6841 aaaaaatcgg aaccttcctg ttgcaattcc ttcctttgat cctcatcata ttttctttgg
     6901 ttctgatcgg caagtcgatc ctttcccttc aaagcggcaa aactccgact attttcaaat
     6961 atgcggtctt atatatcgaa ccggataccg attcgatggt tgatgtgatc atgccgagcg
     7021 acatcatttt cgtcgatacg agagcgcatg attaccagga aggcgatatc atttcctttt
     7081 gggcaaaaga tagagttggc ggcactttgg tgacgctttg tgtgacccat gagatcatcg
     7141 ccattgatac gacaagcggc gaaaaggtct acatcaccca gggagtgaac aacccttctc
     7201 cggattcctg ggcggatggt tgggagataa ccgatgataa aatcatcggg ctctacgtcg
     7261 gccgatcggc tttcctcgga aatgtctaca aactggtagc tgccaacaaa cttggcgtcg
     7321 cctacgccgg cgtcatcatc gtctttttgc tcatcattat atccgagata gcgaatatca
     7381 cgaaagaagt ggcgctcgga aagcagaagc aggcgaccat ggataaggaa aaaatgatcc
     7441 aggaagaact ggaaaaactg aaatccgagc acgaagaccg gaaagataac gggcctttgg
     7501 ccaagtgaca gacaaatatg acggacggta ttttaccgtc ttttttattt attcacggaa
     7561 tcttcttcgt ttcggcatat tctttttggt cctttggacc ggttttttgg tataataata
     7621 ttggatgtga tattttggaa atatatgtgc tggcttcggg atccggcggc aacgccacct
     7681 acgtcaaaat cggaaacacc aacatactga tcgatgccgg aatctccctg cgtcagatca
     7741 cttcccgtct ttcgcagctt gataaaaccc ttgacgggat tgatcatatt tttcttacgc
     7801 atgaacacgg ggaccatata gccggtcttt tgacgatcca gaaaaagtac ggttccgtgg
     7861 tccatatgac catgggcact tacaagaatc ttcccatcag gatttccgac aatctcgaca
     7921 tgagaaaagt caattaccta aattatcttg aacccgtcgt tttagacggc ttttccgtgc
     7981 ttcctttcat gacctatcat gatgccctgg aaccttgcgg atatcgtttc accgaaagcg
     8041 ggaagtcgct tgtctatatg accgacaccg gatattatcc gagaaccgga tacgattgga
     8101 tcagaaatgc cgacatcgtc gtcatcgagt ccaatcacga accggagatg cttctcgaaa
     8161 gcgaaagacc ctggcttacc aaaaaaagga tt
//
LOCUS       DAOPJL010000041         7970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_9635_length_7970_cov_9.197852, whole genome shotgun sequence.
ACCESSION   DAOPJL010000041 DAOPJL010000000
VERSION     DAOPJL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9635_length_7970_cov_9.197852"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLP02_05300"
     CDS             <1..513
                     /locus_tag="PLP02_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPF42891.1"
                     /translation="NMKYPGCVLKGDHAKGTTITIAFAGQGQYQDTGAKMIHIGPYTT
                     STILSKSIAARGGKVNYRGMVSQGPKAVGAKSKVECDTIIVDDISTSDTMPKNIVKNF
                     RGQIEHEATVSKVSEDQLFYLMSRGLSEQEATEMIVMGFIEPFSRELPMEYAVELNQL
                     IKLEMTGSIG"
     gene            complement(529..1830)
                     /locus_tag="PLP02_05305"
     CDS             complement(529..1830)
                     /locus_tag="PLP02_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HPF42892.1"
                     /translation="MTVKACRFTIDRIFAIFVVLLLLFSFTIQPVSEMWAGYLRIISC
                     PSVLISDYIYVGGLGATLFNVATTTGLNLIFLKLMKAKINGSVFACLMTISGFAFFGK
                     NLFNALPIYLGIFLYCLSTKTKVRDHVLVFLLSSGISPVTSFMIFGLGFPLWLGVAVG
                     LSAGVLIGFILPAFNEVVMRFHKGYNLYNTGFSMGVLSMLITGILHSFDIAIYQENEL
                     SNAYHYQFFWFILASSILFIAFAFLKGGKNTIFEYKCLLTKSGRLRTDFALECGWNAT
                     SLNIGIMGLVSVLLVLFTPITINGPIMAAILTIIGFAAFGKHPLNTVPVIIGAILAIE
                     LTPHEWTIGTSLAVLFVTGLAPIAGQFGLLAGVAAGFIHLLITPLALEFQGGFDLYNN
                     GFAAGFVAAVLSSFFNHFLKKRDDKGKWNFLLPLKSKQKAP"
     gene            2111..2434
                     /locus_tag="PLP02_05310"
     CDS             2111..2434
                     /locus_tag="PLP02_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HPF42893.1"
                     /translation="MKLVMAIVSNEDAGKVIKSLIKENFFVTKLATTGGFLMSGNTTV
                     IIGVQDELLDKCIQVISETSRRRTKLVPNAISSEFGIFSSTPVEVQVGGATIFVLDVD
                     KFIKA"
     gene            2557..3114
                     /locus_tag="PLP02_05315"
     CDS             2557..3114
                     /locus_tag="PLP02_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF42894.1"
                     /translation="MENIRVYLYIRGFDRETLFPFLAEFSDLSVTSTDGDYVCLSMKN
                     ETGKEEINHLREKMVEELYQDFVAFIEPNSPNFDTSLVVAIMPKLSAGVYKVTEIIPP
                     VVLKNLTGVKQYLKDYYYWLCGIETIETALGFAEAGLNATKASQSLYMHRNTLNYRLD
                     NFTKTTGIDIRTFAGAYALYLLYKS"
     gene            3206..4333
                     /gene="ugpC"
                     /locus_tag="PLP02_05320"
     CDS             3206..4333
                     /gene="ugpC"
                     /locus_tag="PLP02_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPF42895.1"
                     /translation="MATLSFRHIDKVYDNGVQAVFDFSLEVKDKEFIVFVGPSGCGKS
                     TTLRMVAGLEEITNGELYINDILVNDVAPKDRDIAMVFQSYALYPHMTVYDNMAFGLK
                     LRKMPKMEIEEKVKNAAAILGLEGYLDRKPKALSGGQRQRVALGRAIVRNAKVFLMDE
                     PLSNLDAKLRVAMRAELIKLHEHLNNTTIYVTHDQIEAMTMATRIVVMKDGRIQQIGG
                     PKEIYDNPNNMFVGGFIGTPAMNFLPGTVKKDGYFYGEGLKLLVPEEKVEMLQNLKFI
                     GKPIMMGIRPEDLHDDKISFEKHPESRAKLTVDVSELLGAETNIHITIGDANIIAKVD
                     ARSDLRIGDPIELAFNMHKCHFFDPETEIRMTPDYVKPAQE"
     gene            4400..4867
                     /locus_tag="PLP02_05325"
     CDS             4400..4867
                     /locus_tag="PLP02_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42896.1"
                     /translation="MLYWITGVIAIIDYVKKNQEGFLIVCENGEITAFDIPLKSYLER
                     LAMEGLSTVDGRMKAVGKRFGYRSRIPIYLKKNILFIVLYGLRSPKALLLNYHAVKTC
                     RKIRKGVAISFYSNHEMLYESMARFREAYKKAKAVSEALLDMQDNIESAYMDF"
     gene            4951..6168
                     /locus_tag="PLP02_05330"
     CDS             4951..6168
                     /locus_tag="PLP02_05330"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003739718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPF42897.1"
                     /translation="MNKKTIEDLDVKGRKVLVRVDFNVPMKDGVITDDNRIVQALPTI
                     KYLIEEDAKVILFSHLGRVKTQEDKAKNSLAPVSVHLALLLGRPVKFVPVTRGLALEK
                     AIADMKNGDVVMFENTRFEDLDGKKESGNDPELGAYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASHLESAAGFLMEKELRYIGDAVDNPVRPFVAILGGAKVSDKIGVIENLLTKAD
                     KILIGGGMAYTFMKAEGFQIGTSICEDDKLDLAKELLVKANGKIILPVDVVVAKEFNA
                     DAAYHTTDYKNITSDEMGLDVGEKTIKLFNKELKGAKTVVWNGPLGVFEFPNFAKGTK
                     AICEILANLPDCKTIIGGGDSAAAAIQMGYQDKFTHISTGGGASLEYLEGKALPGVVA
                     LTDAPKCSCGCQD"
     gene            6286..6531
                     /locus_tag="PLP02_05335"
     CDS             6286..6531
                     /locus_tag="PLP02_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004891375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPF42898.1"
                     /translation="MEKTITIKNTAGLHAELAAKIVQAASKYDVDIRMEYEHVTINAK
                     SILGLMSLAVPQGKNVKLVAEGDNAEKAIADIEKLLR"
     gene            6540..7298
                     /gene="tpiA"
                     /locus_tag="PLP02_05340"
     CDS             6540..7298
                     /gene="tpiA"
                     /locus_tag="PLP02_05340"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014822797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPF42899.1"
                     /translation="MRKPIIAGNWKMYKTRDEALFFMMKVASEMPDIREVDTVVCAQA
                     PLLRDLVKRQGDFLRIGAQNVYFEEEGAFTGEVSGLLLKSYNVDYVIIGHSERRNIFH
                     ETDEDVRKKIVAALNADLKPIVCVGEHLEERTEGKTEEILSCQVEKAFAGLKEADMEN
                     IVIAYEPIWAIGTGKTATSEMAEDACKYIRGVVKNLFGNNVAEKIRIQYGGSVKPENI
                     DELLGMDDIDGALIGGASLDPDKFIYMAKAGRKS"
     gene            7411..>7970
                     /locus_tag="PLP02_05345"
     CDS             7411..>7970
                     /locus_tag="PLP02_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42900.1"
                     /translation="MKKIMAIMFVMFFGLVFYSCSNGTTTTIPTDAITSSSTTETTTS
                     ATISTTTTTKVTTTTQVFPTGNFVTLADFTNFSTEFVKDEPITEISFYYFEEKYGIPY
                     VNIEEFINVLLGIIDDSIQVEVNDNVVKVYVLYYYTEDEKAEYGITEDSFEAYVTFDF
                     DTTIVSAPNIDALDYFSGETTTDFSEG"
BASE COUNT         2353 a         1824 c         1980 g         1813 t
ORIGIN      
        1 aacatgaaat accccggatg cgtccttaag ggtgatcacg ccaaaggaac gactatcacc
       61 atcgctttcg ccggccaggg tcagtatcag gacaccggcg ccaaaatgat ccatatcgga
      121 ccttacacga cttcgacgat cctgtccaag tcgatcgccg cccgcggcgg caaggtcaat
      181 tatcgcggta tggtaagcca ggggccaaag gccgtcggcg ccaaaagcaa ggtcgaatgc
      241 gatacgatca tcgtcgatga tatctcgact tcggatacga tgccaaagaa tatcgtgaag
      301 aatttccgcg gtcagatcga gcacgaagcc acggtatcca aagtgagcga ggaccaacta
      361 ttctatctta tgagccgggg actttccgaa caggaagcca ctgagatgat cgtcatggga
      421 ttcatcgaac cgttttcccg tgaattgccg atggaatacg ccgtcgaact caatcaactg
      481 atcaaactgg aaatgaccgg atcgatcgga taaaaaaaca agcgcccgtc aaggcgcttt
      541 ttgtttgctt ttcaaaggta acaggaaatt ccattttcct ttgtcgtccc gttttttcag
      601 gaaatggttg aagaaagagg aaaggacggc agccacaaaa ccagcggcga atccgttgtt
      661 gtaaagatcg aatcctcctt ggaattccag ggccagaggc gtgataagca gatggataaa
      721 gccggcggcg acgccggcta acaggccgaa ctgtccggcg atcggtgcca gtccggtgac
      781 gaaaaggacg gccaaagacg tgccgatcgt ccactcgtgg ggcgtgagtt cgatcgccag
      841 gatcgctccg atgatgactg gaactgtatt gagcgggtgt tttccgaaag cggcgaaacc
      901 gatgatggtg agaatagccg ccatgatcgg tccgtttatg gtgatcggag tgaaaagaac
      961 gagaagtacg gaaacaaggc ccatgattcc gatattgagc gatgtcgcgt tccacccgca
     1021 ttccaaggcg aagtcggtcc gcaggcgacc ggatttagtc agaagacatt tatattcgaa
     1081 aatagtattt ttcccgcctt tgaggaaagc gaaggcgata aacaggatag aagaagccaa
     1141 aataaaccag aagaactgat agtgataggc gtttgacaat tcgttttcct gatagattgc
     1201 aatatcaaac gaatgcagaa taccggtgat gagcatggac aagacgccca tggaaaatcc
     1261 ggtgttgtaa aggttgtatc ctttgtgaaa acgcatcacg acttcgttga aagcaggaag
     1321 gataaaaccg atcaaaacgc cggccgacag tccaaccgct acaccgagcc acagagggaa
     1381 tcccagaccg aaaatcataa agctcgtcac gggcgaaatg ccggaagaaa gcagaaaaac
     1441 cagtacgtga tccctgactt ttgttttggt cgaaaggcaa taaagaaaaa tgcccaaata
     1501 gattggaagg gcattgaata gatttttgcc gaaaaaggcg aaacccgata tcgtcatgag
     1561 gcaggcgaaa accgatccgt tgattttggc tttcatcagt ttcagaaaaa tcaggttgag
     1621 cccagtcgtg gtggccacgt tgaaaagcgt ggcgcccaat ccgccgacat agatataatc
     1681 ggaaatcaaa acggaggggc aggatatgat gcgaagatat ccggcccaca tttccgatac
     1741 tggttggatc gtgaatgaaa acagcagcaa caacaccaca aaaatggcga aaatccggtc
     1801 gattgtgaag cggcaggctt tgacggtcat ggtttaggtc ctccctatgc ttgtcgtaag
     1861 gaaaagagat aaaggatggc tgtattgcga cctgtcgcgg ccgatacggc cgcttgtgcc
     1921 gatctcaata tactatatcg cgccaatttg gtaaacaagc cggtcgtttt tttgtatgaa
     1981 aaagcgtgta actcgcgcaa aatcaatttg aaaatagctt tgaaaacccc aaacaaggct
     2041 gttttttgcg ttattctatt ttatttggaa taatcatgtg atagaatata ccaaaaggtg
     2101 gtgatgtgcc atgaaactgg tgatggccat tgtttccaac gaagatgccg gcaaagtgat
     2161 caaatcattg atcaaagaaa atttcttcgt taccaaattg gcgacgaccg gcggctttct
     2221 catgagtggg aacacgacgg tgatcattgg cgtccaggac gaacttctgg acaaatgtat
     2281 ccaagtcatc agcgaaacat ccaggcgcag gaccaaactg gtccccaatg ccatttccag
     2341 cgagttcggg attttttcct cgactccggt ggaggtgcag gttggcggag ccactatctt
     2401 cgtcctagat gtcgacaagt tcatcaaagc ctgacaacgt ttgtttaaga aaagatccga
     2461 tggaaatcca ttggatcttt tttctattcc cggtctttat atatccaagg gtttttggta
     2521 taatgaatat actaaagcat gcaaggaagt gtcggcttgg aaaacatcag ggtttatttg
     2581 tacattcgcg gattcgatcg ggaaacactg tttccttttt tggctgaatt cagcgatctg
     2641 agcgtgacat cgactgacgg cgattatgtc tgcttaagta tgaagaacga aacgggaaaa
     2701 gaggaaatca accatttgcg cgagaaaatg gtcgaagagc tttaccagga cttcgtcgcc
     2761 ttcatcgaac cgaattcccc gaatttcgat accagtttgg ttgttgcgat catgccaaaa
     2821 ttgtcggcgg gggtgtataa ggtgacggaa atcattccgc cggtagtcct gaagaacctt
     2881 accggagtca aacagtatct taaggattac tattactggc tatgcggcat cgagactatc
     2941 gagacggcct tgggattcgc cgaggcgggg ttgaacgcca ccaaggcttc tcaatcgctt
     3001 tacatgcaca ggaacaccct taattaccgt ttggacaatt ttaccaagac gaccggcatc
     3061 gatatccgga cttttgccgg ggcgtacgcc ctttatttgc tttataagtc ctgattttgt
     3121 gcagtttgca tataggatca ggtctttttt tttggtaaaa tataagacga ggaaaaatta
     3181 tttcaacaat taacggaggt aaaaaatggc cacattatca ttcagacaca tcgataaggt
     3241 ttatgacaac ggcgtccagg cggttttcga tttttccctg gaagtcaaag acaaggaatt
     3301 catcgtattc gtcggaccgt cgggctgcgg aaaatccacg accctgcgta tggtcgccgg
     3361 actggaagaa atcaccaacg gcgaacttta catcaatgac attctggtta acgacgtcgc
     3421 accgaaagac cgcgacatcg ccatggtttt ccaatcctac gccctttatc cgcacatgac
     3481 cgtctacgac aacatggctt tcggactcaa acttcggaaa atgccgaaaa tggaaattga
     3541 ggaaaaagtc aaaaacgcag ccgccattct gggactggaa ggctatcttg acaggaagcc
     3601 aaaagccctg tcaggcggtc agagacagcg tgtcgccctg ggaagagcta ttgttcggaa
     3661 cgccaaggtg ttcctgatgg acgaaccttt gtccaatctc gacgccaaac tgagagtcgc
     3721 gatgcgcgcc gaactcatca aattgcacga gcatctgaac aatacgacga tttatgtcac
     3781 ccatgaccag atcgaagcca tgaccatggc tacgagaatc gtcgtcatga aagacggtcg
     3841 gatccaacag atcggcggac cgaaggaaat ctatgacaat ccgaacaata tgtttgtcgg
     3901 cggatttatc ggaacaccgg ccatgaactt cctgccagga accgtcaaga aggacggata
     3961 tttctatggc gaaggactca aactgttagt tccggaagaa aaagtcgaaa tgcttcagaa
     4021 tctcaaattc atcggcaaac cgatcatgat gggcatccgt ccggaagatt tgcacgacga
     4081 caagatctcc ttcgaaaagc atccggaatc cagagccaaa ctgacagtcg atgtttccga
     4141 actcttggga gccgaaacca atatccatat cacgatcggc gacgcgaaca tcatcgccaa
     4201 ggtcgatgcc agaagcgatc tgcgtatcgg cgatcccatc gaactcgcct tcaatatgca
     4261 caaatgccat ttcttcgatc cggaaaccga aatcagaatg acaccggatt atgtgaaacc
     4321 cgctcaggaa taaagccaac aagccacctt atgggtggct ttttttatcg tttttttcaa
     4381 gccttcggtg ccaatcgacg tgttatattg gataacgggg gtgattgcga tcatcgatta
     4441 tgtcaaaaaa aaccaagaag gttttttgat cgtctgtgaa aatggagaaa ttacagcttt
     4501 cgatattcct cttaaaagtt atttggaacg tctggcgatg gaaggtttat cgacagttga
     4561 cggacgaatg aaggcggttg gcaaacgttt cggttatcgt tctcggattc cgatctatct
     4621 gaaaaaaaac attctgttca tcgtccttta cgggctgcgt tcgcctaaag ccctgctttt
     4681 gaactatcac gccgtgaaaa cctgccgaaa gatccgaaaa ggcgtggcga tatcttttta
     4741 ttcgaaccat gaaatgcttt atgaaagcat ggcaaggttc cgggaagctt acaagaaagc
     4801 aaaagcggtt tcggaagcct tactcgacat gcaagacaac atagaaagtg cttacatgga
     4861 tttttaaccg ccattttaga gtggaaatac ataccaaaaa atagtataat aaagcgtgtt
     4921 gaaaatataa tatgtcttgg aggtttcatt atgaacaaga aaaccatcga agatttggat
     4981 gtcaagggcc ggaaggttct tgtaagggtc gatttcaacg tccctatgaa agacggagtc
     5041 atcaccgatg acaaccgtat tgtacaggca ttaccgacaa tcaagtatct gatcgaagaa
     5101 gatgcgaaag tgattctctt cagccatctc ggcagagtca agacacaaga agacaaggca
     5161 aagaactcac tggcccccgt cagcgtccat ctggccttgt tgcttggacg tccggtcaaa
     5221 ttcgtgccgg tgaccagggg attggctctt gagaaagcaa tcgccgacat gaaaaacggc
     5281 gatgttgtca tgtttgagaa taccagattc gaagacctgg acggcaagaa agaatccgga
     5341 aacgatcccg agttgggagc ctattgggca tcacttggcg acgtgttcgt gaatgacgcg
     5401 ttcggaaccg cccatcgcgc ccacgcttcc aatgtgggta ttgcctcgca tctggaatcg
     5461 gctgccggtt tcctgatgga aaaggaattg cgctacatcg gcgacgccgt tgacaacccc
     5521 gtaagaccgt tcgtcgccat tcttggcgga gccaaggttt ccgacaaaat cggtgtcatt
     5581 gagaatctac tcaccaaagc cgacaagatt ctgatcggcg gcggcatggc ctacacattc
     5641 atgaaagcgg aaggattcca gatcggaaca tcgatctgcg aagacgacaa actcgacctc
     5701 gccaaggaat tgcttgtcaa ggccaacggc aagatcattc tgcccgtaga cgtcgtcgtc
     5761 gccaaggaat tcaatgccga cgccgcttac catacgaccg attacaaaaa catcacatca
     5821 gacgaaatgg gtctcgatgt cggtgaaaaa acaatcaaac ttttcaataa ggaacttaag
     5881 ggagccaaga cagtggtctg gaacggaccg ctcggagtct tcgaattccc gaatttcgcc
     5941 aaaggcacca aagcgatctg cgaaatattg gccaacctgc ccgattgcaa gacaatcatc
     6001 ggcggcggcg attccgcagc tgcggccatt caaatgggat atcaggacaa attcactcat
     6061 atctcgaccg gaggcggcgc atctttagaa tatctggaag gcaaggcatt gccgggcgta
     6121 gtcgccctaa cggacgcccc gaaatgctct tgcggctgcc aagactgaca aaccagaaac
     6181 cataggtggg acaaaacaca aagcccatgg tccgtcacgg accgggtttt gtattgaatg
     6241 gaacagccgg aaaccggctg gttcaaacat tttaaggaga agaagatgga aaaaacaatc
     6301 acaataaaaa acacggccgg gcttcatgcg gaactggcgg ccaaaatcgt tcaggccgca
     6361 agcaaatacg atgtcgacat caggatggag tacgagcacg tgaccatcaa tgccaagtcg
     6421 atcctcggtt tgatgagcct tgccgtgccg cagggaaaaa atgtcaaatt ggtggcagaa
     6481 ggcgacaacg ccgaaaaggc gattgcagat atcgaaaaat tattaaggta ggcgataacg
     6541 tgagaaaacc gatcattgcc ggaaactgga aaatgtacaa aacccgtgac gaagcacttt
     6601 tctttatgat gaaagtcgca tctgaaatgc cggacatcag agaagtcgac acggtggtgt
     6661 gcgcccaggc accattgctt cgcgatcttg tcaaacgaca gggcgacttt ttgcgaatcg
     6721 gcgcccagaa cgtgtatttc gaggaagaag gggcttttac cggcgaagtc tcgggcttgc
     6781 tactcaaaag ctacaatgtc gattacgtaa tcatcggaca cagtgaaaga agaaatatct
     6841 tccatgaaac cgacgaagat gtcagaaaga agatcgtcgc cgctctgaat gcggatttga
     6901 aaccgatcgt ctgcgttggc gagcatttgg aagaaagaac ggaaggcaaa accgaagaaa
     6961 ttctctcttg tcaggtcgaa aaagcctttg ccggcctcaa ggaagcggat atggaaaaca
     7021 ttgtgattgc ctacgaaccg atttgggcga tcggaaccgg taaaaccgcg accagcgaaa
     7081 tggccgaaga cgcatgcaaa tacatccgcg gagtcgtaaa aaatctgttt ggaaacaatg
     7141 ttgccgaaaa gatcagaatt cagtacggcg gctccgtaaa accggaaaac atcgacgaat
     7201 tgcttgggat ggatgacatc gacggcgcct tgatcggcgg agcttcgctc gatccggaca
     7261 aattcattta catggctaaa gccggaagaa aaagttaatg aaaacaaaga accgaaaagt
     7321 tttcggttct ttttttgcaa tccgattgaa ttatttataa aatacggtaa aataatgata
     7381 atcgtttcat accggaggag gaaataaaat atgaaaaaaa ttatggctat catgttcgta
     7441 atgttttttg gactggtttt ttacagttgc agcaatggaa cgacaactac gattccgact
     7501 gatgctataa cgtcgtcaag cacgacggaa acgacgacat cggccaccat aagcacgaca
     7561 accacgacca aggtcacaac cacgacccag gttttcccga cgggcaattt cgtcacattg
     7621 gccgacttca ccaatttctc gacggaattc gtcaaagacg agcccatcac cgaaatttct
     7681 ttctattatt tcgaggaaaa atacgggatt ccttatgtca atatcgagga attcatcaac
     7741 gtgcttctgg gaatcatcga tgattccatc caggtggaag tgaacgacaa tgtcgtgaaa
     7801 gtatacgtac tttactatta taccgaagat gagaaagcgg aatacggcat caccgaggat
     7861 tcgttcgaag cttatgtcac attcgatttc gatacgacca tcgtgtcggc gccgaacatc
     7921 gacgccctgg attatttctc cggcgagacg acaaccgatt tctccgaagg
//
LOCUS       DAOPJL010000042         7636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_10244_length_7636_cov_4.082443, whole genome shotgun sequence.
ACCESSION   DAOPJL010000042 DAOPJL010000000
VERSION     DAOPJL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10244_length_7636_cov_4.082443"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..935)
                     /locus_tag="PLP02_05350"
     CDS             complement(165..935)
                     /locus_tag="PLP02_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPF42901.1"
                     /translation="MTNINDEYILETFFVNVPDLGYSKKIYACMPETSKECPVLYMHD
                     GHNLFLPEDSFNGSTWQAKSALNELYNNTGKHLAIIGIGADHRRFDEYSPWQGCLEFE
                     KNGGLGDIYLEWIIKELIPLIRQKYNFGTRYFMAGSSMGGYISMYAGYKYRNMFEKVG
                     LFSPAFWFNFQKMETFVKNNFDPNLGMYLDVGKKETSDVNRTDFPDIYLSDARKFHNL
                     LNVLGIKNIKYLEDEEGQHSEKDWAKRFPGFLNWLLRP"
     gene            complement(919..1926)
                     /locus_tag="PLP02_05355"
     CDS             complement(919..1926)
                     /locus_tag="PLP02_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPF42902.1"
                     /translation="MLNKKEWLEKIEVEKKNSMPILTFPAIQLFGITVKQLVSSSDLQ
                     AKVMAAIAEKYPVSAVLSMMDLSVEAEEFGATVRFFDMDVPTVTGRVINDMDAAKKLM
                     VPLLGGKRTLQYVEGIRKAKALIPDKPIFAGVIGPFSLAGRLMDMTEIMVNCYLEPEM
                     VHITLEKATEFIIKYIKAFKLAGADGVVMAEPAAGLLSPEICEEFSSRYIRRIVKETA
                     DDKFVFVYHNCGNTIPLHETIASINADIYHFGDAIDLEEMLKVFPADKLVMGNISPAR
                     VIRNLEPKKIKEEVTNLLKRCSKYENFSISTGCDIPPMTPLENIEAYFSAISEYYDKH
                     Q"
     gene            complement(1916..2971)
                     /locus_tag="PLP02_05360"
     CDS             complement(1916..2971)
                     /locus_tag="PLP02_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPF42903.1"
                     /translation="MIRPASWIPDFHNLELVLKKQKPPRPVMFDFIIGDEKTRMLAKE
                     DYRDETELDRVVTTLKAFENAGYDHSPIIVRGLDFTRKTDRSHQNVQTKSLNSGATIT
                     SRETFESYKWPELSDCDFSIIHKAGKHLTKGVLFVPFSLDGILENTIGIVGYENLCYM
                     LYDDYDLAADIFKNVGTRIAEYFEICLNYSEVGAVLLNDDWGFNSQTMVPPDILRKHV
                     FPWYQKVAEKAHKMGKYALLHSCGYYQDIIDDIIDVIKIDGRHSYEDKIVPVENAYRE
                     LNPRLAILGGMDVDFLSRSQPSQIYARCVKMLKLSAKSGGYALGSGNSIPDYISNENY
                     LAMLKAAYDMEDEYNVK"
     gene            complement(2997..4577)
                     /locus_tag="PLP02_05365"
     CDS             complement(2997..4577)
                     /locus_tag="PLP02_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42904.1"
                     /translation="MNKIARMTSAMVFLFLLAMALVGCKGETTTATTTAGTTTTTTTT
                     EAETTWYLAGNFSGYVADDSDYVMSPVTGMDGWYTITVSLTADNRDNAYDGHYYKVTD
                     GTWTTCYGASNYAMQPAPVSPTGGGLGSVWVYENMDLTVMFDSNTLTIYDNSMVEDME
                     NPRIYGDFSAAMGLGSDWSSDASANLVLTDPDEDGIYEGEWTIPAYAGTSENGYSFDV
                     ATQRQYYINEWGNYWGVTVQYLFDGSAAGMGQSSYLKPTTDTVYHFAYNSTTHVTTVT
                     EILASPIIYGDFSGWSLDPANGAILLVQSATEEDLYSGTSTIPAYTGSGEAYMMAVCL
                     TKQLYYWEGVGSWGAGEQYLFSGDAGGMGKVTYLYLDEAMELTFTYDASDNTTEITPP
                     AGKSVGPYEYLSGPTLYGTFTRVTMDSECWILEGTGAAVMTAVSGDATKYEVTLQLNA
                     YVAPTEGYYTEDTGYRFLLVTTKADYGWGYYAGTQYTFDGNLAGMGGYTNITITETGT
                     YHFVYDSVTHITTYEKIG"
     gene            complement(4574..6313)
                     /locus_tag="PLP02_05370"
     CDS             complement(4574..6313)
                     /locus_tag="PLP02_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPF42905.1"
                     /translation="MQKTAILHIQEYPYCIVTGPDALTLRIRAAKDDIDQAFICFKNL
                     YDHTSEFQKVEMKRILASALFDVYEATIKVPLKHFKYYFELIFGSAKIYYTSDGFLEI
                     VAPENCFYYPNINSDEILALPKWAEGETIYQVLVDRFFDSDPSNNPPQTKPCKAIPDR
                     NTYYGGDFGGIIAKLDYLASYGVGIIYLSPVLKSPSYHKYDVEDYYTIESIYGGKEKL
                     RELTEEAHKRGMKVILDGVYGHCSCEHEFFRDLCEKNEKSPYRNWFEVYGFPVSVEKG
                     NYNTFANVVPIMPKLNTANNEVIAYFAKQGAYWAKYLDIDGYRLDVADEISHTFWRQF
                     RLSLEAVKKDALLLGEIWNQPTQWLKPGELHSATNYKFRQYTLALARNEIGTKKYASL
                     IEEHLMTTSTMQQNYLASLLGSHDTLRLANEIPERKTRFLIMKLMLLMPGMPLIYYGD
                     EISLAGGKDPDNRRAMDWNHMLTKEETEFREAAMFRKKSEILKKGSLNFLTSEEGLLV
                     FTRELAAEELIVIANFTDVSMDYEIEDEYKLVLGKIATIPGKIRLDAKEIAVLQMDKA
                     NNTLEKIKRREKG"
     gene            complement(6326..7159)
                     /locus_tag="PLP02_05375"
     CDS             complement(6326..7159)
                     /locus_tag="PLP02_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF42906.1"
                     /translation="MKKNRKIIKKTLIQIFLIFMALVTILPFAYIIMIAFGKNIVGTS
                     ATIPTEFTFDNFNRLFAETEFLSWAMNSLVISLVTMVVATIFVSISVYVFSRLQFRGR
                     RGLFQFILLIQIFPLTLSMVSIFRIFVALGLLNDLKGLIIVDSTISSAGLVLLAKGYF
                     DTISYELDEAAMVDGANRFQILTKIIMPLAKPMLAVVAIQSFVIAYNEYVIASTVMTQ
                     GLDTMPLAVGLQSMITGQYGTNWSLYCAAAILGSVPMILLFYCLQKYFIGGLTEGSVK
                     M"
     gene            complement(7161..>7636)
                     /locus_tag="PLP02_05380"
     CDS             complement(7161..>7636)
                     /locus_tag="PLP02_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF42907.1"
                     /translation="FGIPNIPWLSDSFLAKISTIMVMTWFSFPYFMVVASGFLQAIPK
                     DYYDAAKVDGASNRHIFFHITLPLIFRAMVPVLIMSFIMQFNQFGVYILTSGGPASST
                     IGAPGATDLLITYVFNTAFNTKRYAVAAAYSVVIFVFVSIFALVSMKVTRKVAES"
BASE COUNT         2007 a         1712 c         1684 g         2233 t
ORIGIN      
        1 tgcaatattc cacctttttc gccaaatgat atcttacgct ttttcataat tatattatac
       61 aatgaaagcg cttgtttaac aagcaaattt tttggtaatt tttcttacgc ttctgtcaat
      121 gatgttaaat taggttttcc gctacttaac caatgtggct tatttcatgg acgcaaaagc
      181 caattgagga aaccgggaaa tctttttgcc caatcctttt cagaatgctg gccttcttca
      241 tcttccagat acttgatgtt ttttattccg aggacattta ggagattatg gaactttctt
      301 gcatcggaaa gataaatatc cggaaagtca gtacgattca cgtcgctggt ttcctttttc
      361 ccaacgtcca gatacattcc taaattagga tcgaagttat tctttacaaa tgtttccatc
      421 ttttgaaaat tgaaccaaaa tgctggcgag aataatccga ctttttcaaa catgtttctg
      481 tacttatatc cagcatacat gcttatatat ccgcccattg atgatccggc cataaagtag
      541 cgagtgccga aattgtactt ttgtctgatc aagggaatca attccttaat gatccattcg
      601 agatatatgt cgccaagacc accgtttttt tcgaattcca aacaaccttg ccaaggcgaa
      661 tattcatcaa accgtctgtg atcagcgcca ataccgatga ttgcaagatg ctttccagtg
      721 ttattataaa gctcatttaa agcgcttttt gcctgccaag tgctgccgtt aaacgaatct
      781 tctggcaaaa acaggttatg tccatcatgc atatagagaa ccggacattc tttagacgtt
      841 tcaggcatac atgcatagat tttttttgaa tacccgaggt cgggaacatt tacaaaaaat
      901 gtctcgagaa tgtattcgtc attgatgttt gtcataatat tcagaaatag cggaaaaata
      961 cgcttcaata ttttcaagcg gcgtcatggg aggaatgtcg caaccggtgc tgatagagaa
     1021 gttttcatat ttggaacatc ttttcaacaa gtttgtgact tcttccttta tttttttcgg
     1081 ttccagattt cgaataaccc tcgccggact aatgttgccc atgaccaact tgtcagcggg
     1141 gaagacttta agcatttctt ctaaatcaat agcatcgcca aaatgataga tgtccgcatt
     1201 tatcgaggca atggtttcat gaagcggaat cgtattaccg caattgtgat aaacgaaaac
     1261 aaacttgtcg tcagccgttt ctttgactat ccggcgaata taacgcgatg agaattcctc
     1321 gcatatctcc ggagacaaca aaccggcagc cggttcggcc atcacaacgc catcggctcc
     1381 ggccagttta aaggctttga tatatttgat tatgaattct gtcgctttct ccaaggttat
     1441 gtggaccatt tccggttcca gataacaatt taccattatt tcggtcatgt ccattagcct
     1501 tccggctaaa gagaatgggc cgatgactcc ggcaaaaatg ggtttatcgg gaatcaaagc
     1561 ttttgctttg cggatgcctt cgacatattg gagtgtgcgt ttacctccga gaagtggaac
     1621 catgagtttc tttgcggcat ccatgtcgtt tatgacacga ccggtaacgg ttggcacatc
     1681 catatcgaaa aaccggactg tagcgccaaa ctcttcggct tccacgctca aatccatcat
     1741 tgacagtacg gcgcttactg gatatttttc ggcgatagct gccatcactt tggcttgcag
     1801 atcggatgac gataccagtt gtttaaccgt tatcccaaaa agttggattg ccggaaaagt
     1861 caaaatcggc atggagtttt tcttttcaac ttcgattttt tcgagccatt cttttttatt
     1921 taacattgta ttcatcctcc atgtcatagg ctgctttcaa catcgccaga tagttttcgt
     1981 tggagatgta gtctgggatc gaattacccg atccgagagc gtatcctcca cttttggcgg
     2041 acaattttag catcttaaca caacgtgcgt aaatctggga aggttggctg cgggaaagga
     2101 aatccacatc catgcctccg agaattgcta atctagggtt aagttcccga taagcgtttt
     2161 cgacgggaac gattttgtct tcataggaat gacgaccatc gattttgatg acatcaatga
     2221 tgtcgtcaat gatatcctga taatatccac atgaatgtaa caaggcgtat ttgcccatct
     2281 tatgggcctt ttcagccacc ttttgatacc atgggaatac atgctttcgc aaaatgtccg
     2341 gggggaccat cgtttgggaa ttgaatcccc agtcgtcgtt caaaagaacg gcgccgactt
     2401 cggaataatt caaacagatt tcaaagtatt cggcaatcct agtgccgacg ttcttgaata
     2461 tgtctgctgc taagtcgtag tcatcatata gcatgtaaca aagattctca tagccaacaa
     2521 tgccgattgt gttttccaat attccatcta gactgaaagg aacaaaaaga acgccttttg
     2581 tcaagtgttt tcctgcttta tggataatcg aaaaatcaca gtcggacagt tccggccatt
     2641 tgtaactttc aaaggtttcc cgcgacgtga tggtcgcacc gctgttcaag gatttagttt
     2701 gaacattttg gtgcgaccta tccgtctttc gggtaaagtc aagtcctcgg acaatgatgg
     2761 gtgaatggtc ataaccggcg ttttcaaaag ctttcaaggt ggttacgacg cgatccagtt
     2821 cggtctcgtc acggtaatct tctttagcaa gcattctagt tttttcatcg ccgatgataa
     2881 aatcaaacat tacgggccgc ggtggttttt gctttttgag tactagttcc agattatgga
     2941 aatccggaat ccaggatgcc ggtctgatca tttctctcac cttttttccg ggaatcttat
     3001 cctatttttt cgtatgttgt gatatgggtg acgctgtcat aaacaaaatg atatgtgcct
     3061 gtttcggtaa ttgtaatgtt ggtatatcca cccattccgg ccaaatttcc gtcgaaggtg
     3121 tactgcgtgc cagcataata gccccatccg taatcagctt ttgtcgtaac gagcaagaag
     3181 cgatagccgg tatcttcggt ataatatcct tctgtcggag caacataggc attcaactgc
     3241 agggttactt cgtatttggt cgcatcaccg gacactgctg tcatgacagc ggcgccagtg
     3301 ccctccagaa tccagcattc ggaatccatg gttacgcggg taaacgttcc gtatagtgtg
     3361 ggtccggaca gatattcgta tgggcccact gatttacctg cgggtggggt aatttcggtt
     3421 gtgttgtcgg aggcgtcata ggtgaaggta agttccatcg cctcgtccaa gtaaagatag
     3481 gtgacctttc ccatgccacc ggcgtcgccg ctgaacagat actgctcacc ggctccccat
     3541 gagccaacgc cttcccagta ataaagttgt ttggtcaagc aaactgccat catgtacgct
     3601 tcgcccgatc ccgtgtatgc cgggatggtg gaagtgccgg aataaaggtc ttcttccgtg
     3661 gcggactgga ccaacagaat cgcgccgttt gccggatcaa gcgaccagcc ggagaaatct
     3721 ccgtaaatga tcggacttgc taatatttca gtcacggtcg ttacatgtgt ggtggagtta
     3781 tatgcgaaat gatatactgt gtcggttgtc ggtttgagat aggaagactg tcccattccg
     3841 gctgcggatc cgtcaaataa atactggacg gtgacgcccc aatagttgcc ccactcgtta
     3901 atataatact gtctttgggt ggcgacatcg aacgaatacc cgttttctga agtgccagca
     3961 tatgctggaa ttgtccattc gccttcataa atgccgtctt cgtccggatc tgtcaaaacg
     4021 aggttagcgc tggcatcgct gctccaatcg gagccaaggc ccatagcggc gctgaagtcg
     4081 ccataaatcc gtgggttctc catatcttcg accatcgaat tgtcatagat cgtcagagtg
     4141 ttagagtcga acatcacggt gagatccatg ttttcataga cccataccga accgagtccg
     4201 ccgcctgttg gggaaaccgg agccggctgc atagcgtaat tgctggcgcc atagcaggtg
     4261 gtccaagttc cgtcggttac tttataatag tgaccgtcgt aggcattgtc acggttgtcg
     4321 gcagttaggc ttaccgtaat ggtataccat ccatccattc cggttaccgg actcattaca
     4381 taatcggaat catcggccac gtagccggaa aaattaccgg caaggtacca agttgtttcc
     4441 gcttccgtgg tggtcgtcgt cgttgtggta ccagcggtag tggtggctgt tgtcgtttca
     4501 cctttgcagc caaccaaggc catggccaat aaaaacagaa aaaccattgc cgaagtcatt
     4561 cttgcaattt tattcatcct ttttctctcc tttttatttt ttccaacgta ttgttggcct
     4621 tatccatttg taatactgct atctctttag catcaagtct tattttcccg gggattgtcg
     4681 caattttgcc caaaaccaac ttgtattcat cttcaatttc gtaatccatg cttacgtcgg
     4741 taaaattggc aataactatg agttcttccg ctgctagttc cctggtaaag accagcagcc
     4801 cctcttctga ggttagaaaa tttagggaac ctttttttaa gatctccgat tttttgcgaa
     4861 acatggctgc ctcgcgaaac tctgtttcct cttttgtcag catatggttc caatccatcg
     4921 cccggcggtt gtccgggtct tttccaccgg caagtgatat ttcgtctccg tagtatatca
     4981 gcggcattcc cggcatcaaa agcatcagtt tcattataag aaagcgggtt tttctttctg
     5041 gtatttcatt tgccaatcgc agggtatcgt gagagccgag cagacttgcc agataattct
     5101 gctgcatagt cgaagtcgtc attagatgct cttcgatcag ggatgcatat ttttttgtcc
     5161 cgatctcgtt tctggctaat gccagcgtgt actgtctgaa tttgtagttg gtcgcggaat
     5221 gcaactctcc gggttttagc cattgggttg gctgattcca aatttctccc agcagtaaag
     5281 catctttttt caccgcttct aggcttaacc tgaattggcg ccagaaggta tgactaattt
     5341 catccgccac atcgaggcgg tagccgtcga tatccaggta ttttgcccaa tatgctcctt
     5401 gtttggcaaa ataagcgatc acttcattat tagcagtatt taattttggc atgatcggca
     5461 caacattggc aaaggtattg taattacctt tttccaccga cacgggaaag ccgtaaacct
     5521 caaaccagtt tcgatacggg gatttctcgt ttttctcgca aaggtcgcgg aagaattcat
     5581 gttcgcatga gcagtgtccg taaacaccgt caaggataac tttcatgcct cttttgtgag
     5641 cttcttcggt tagttcccga agtttttctt ttccaccata gattgattcg atcgtataat
     5701 agtcttcaac atcatatttg tgataactcg gagactttaa gacgggagaa agataaatta
     5761 ttccaacgcc gtaacttgca aggtagtcca gtttagcaat gatgccgcca aaatccccgc
     5821 cataataggt gttacgatca ggaatggcct tgcatggttt tgtttgggga ggattgtttg
     5881 acggatcgga atcgaaaaag cgatcgacaa gaacttgata gatcgtttcg ccttccgccc
     5941 atttgggtaa ggcgagaatt tcgtcactgt ttatattggg gtagtaaaag cagttttcgg
     6001 gagcaactat ttccagaaag ccatccgatg tgtaataaat cttagcggat ccaaaaatca
     6061 gttcgaaata atatttaaaa tgtttcaaag gtacttttat cgtggcttca tagacatcaa
     6121 acaatgccga tgccaatatt cgtttcattt ctactttctg aaattccgat gtgtggtcgt
     6181 aaagattctt gaagcaaatg aaagcctgat cgatgtcatc tttggcggct cttattctca
     6241 aagtcagggc gtcaggtccg gttacgatgc aataaggata ttcctggata tggagaatag
     6301 ctgtcttctg cattatcatt tcctcctaca tctttaccga gccttcggta aggccgccga
     6361 tgaaatactt ctgcagacaa taaaacaaca gaatcatcgg tactgatccg agaatggcag
     6421 cggcacagta taagctccag ttggttccgt attgcccggt tatcatcgat tgcagtccga
     6481 cagcaagcgg catggtatcg agcccctggg tcatcacggt cgaagcgatc acatattcgt
     6541 tataggcaat aacaaaactt tgaatggcca caactgccaa catcggtttt gctaaaggca
     6601 tgatgatttt cgtcagaatc tgaaaacggt tggcgccatc aaccattgcc gcttcgtcaa
     6661 gttcatagga tatggtgtcg aaatatccct tcgcaagtag cacaagtccg gccgaggaga
     6721 tggtcgaatc gacaataatc agtcctttga gatcgttcaa aagtccgagt gccacaaaaa
     6781 tgcggaatat cgataccatc gagagggtga gcggaaaaat ctgaatcaat aagataaatt
     6841 ggaaaagtcc tctcctgcct ctaaattgca gacgggagaa cacgtagaca ctgatcgata
     6901 cgaaaatcgt ggctacaacc atggttacga gcgatatcac aagcgaattc atggcccagg
     6961 acaaaaactc ggtttccgca aacaggcggt tgaagttatc gaatgtaaac tcggtcggaa
     7021 tcgtggcgct ggtgccgaca atgtttttcc cgaaagcgat catgatgatg tacgcaaacg
     7081 gcaaaatcgt caccaaagcc atgaatatga gaaagatctg aataagcgtt ttcttgatta
     7141 ttttcctgtt tttcttcatt tcacgattcc gccacctttc ttgtcacctt catcgatacc
     7201 agcgcaaaga tggaaacgaa aacgaaaatt accacggaat atgctgccgc aaccgcatat
     7261 cgtttcgtat tgaaggctgt gttgaacaca tatgttatca aaagatcagt agcacccgga
     7321 gccccgattg tgcttgaggc ggggccgccg gaagtcagaa tataaacacc aaattggttg
     7381 aattgcatga tgaaagacat tatcaatacc gggaccatag cccggaaaat aagcggcaag
     7441 gtgatatgga aaaatatgtg tcggttagac gctccgtcaa cttttgcagc gtcatagtag
     7501 tcctttggta tcgcttgtag aaagccagag gcaacaacca tgaaataagg gaacgaaaac
     7561 caagtcatga ccatgatggt cgatattttg gcaagaaaac tgtcggacag ccaaggaatg
     7621 ttcggaatac cgaata
//
LOCUS       DAOPJL010000043         7015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_11441_length_7015_cov_7.519971, whole genome shotgun sequence.
ACCESSION   DAOPJL010000043 DAOPJL010000000
VERSION     DAOPJL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11441_length_7015_cov_7.519971"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLP02_05385"
     CDS             complement(<1..442)
                     /locus_tag="PLP02_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42908.1"
                     /translation="MNIKEIIENFKNKPSKEKVKTIIVVAVIFICLFLGMFAPRLFPN
                     TVFANIIANSLGKFFDLITFFSNNYITLLECVAVIVFVWVLNKVLAWLVNILAKKGHR
                     SETIGNLLKSVVKYLSYIVAICLILSAWNVQTATLLAGAGIIGLA"
     gene            complement(460..1800)
                     /locus_tag="PLP02_05390"
     CDS             complement(460..1800)
                     /locus_tag="PLP02_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42909.1"
                     /translation="MARMVEFREKIKKKTRFEAFKSIFSSLTVTVAAVVIAATVIPKS
                     AVASFAAIEPYANEIIYHVDVTDEENAIIAGTLKVVLECQDEYYEQSLELGTSSGIFT
                     GLKTNTEYSIRVEADKGFGTEYLVNDKVKTESRAGGAITGYELISSGETSYLEYDIGF
                     FLADPETELTGFTMTYYVLMDKEYIVEEETTVQLLPNETSVSILVPYYDNSSVYIEIS
                     GEYVNGEKTIFDQLTFDEPLYYMGSFYIEQVTNDMISVIAYAEPYGIEDIRYELHLQH
                     NKVDLQVIEITPSSMEEGYMHECGEEIVFSNLRHNRRYDIKLFAYYQDPTTLAYVERV
                     VYEESVTTMQDFDCNVDVTETIDAYLVTVTIVDSSDTFQNAYYGIYQTVDYGDGYIYD
                     QLEFWRDSDFTVNAHSKIVMFTINKPDYEDWHIEIGISSDTEYYYYYIVTEITP"
     gene            complement(1939..2015)
                     /locus_tag="PLP02_05395"
     tRNA            complement(1939..2015)
                     /locus_tag="PLP02_05395"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1979..1981),aa:Pro,seq:ggg)
     gene            complement(2105..2719)
                     /locus_tag="PLP02_05400"
     CDS             complement(2105..2719)
                     /locus_tag="PLP02_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF42910.1"
                     /translation="MTDISLDQKQCGLLENAAAYVKTIMENDEPGHDFSHIERVVGNA
                     RKILAKEPADPFITLMVAYLHDLDDPKLQRKETNLAKKYLDSCDLDSVIKTKILKAIS
                     EISFSGQKNGIKITSKEAAIAQDADRLDALGAIGIARTFSFGGHLGRKIYSGRVDDDS
                     SFAHFYQKLFLLVNLMNTKTAKRIAKSRTKYMKKYVRRLARETS"
     gene            complement(2712..3215)
                     /locus_tag="PLP02_05405"
     CDS             complement(2712..3215)
                     /locus_tag="PLP02_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPF42911.1"
                     /translation="MAVYSYKGINPAISGKAKVFPSATVCGDVKIGDNSSVWFGSVVR
                     GDMAKITIGRNTNIQDNAIVHTSIDHPTMIGNNVTIGHGAIIHGCTIGDNVLVGMGAV
                     ILDGAVIEKNAMVAAMALVPQGKTVPEGMLALGVPMIVKRSLTAEEIETNYENSLEYM
                     KMAVEYD"
     gene            complement(3307..5118)
                     /locus_tag="PLP02_05410"
     CDS             complement(3307..5118)
                     /locus_tag="PLP02_05410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF42912.1"
                     /translation="MIIVFVIDNYGELSNGTTMTAKRTKEMLEKRGHTVRIVSAGPII
                     GEGYYQLKKRWIPIVSEVSAKQHMYFAKFDRKVMEKAFEGADIVHFFLPFSVSIKGIK
                     LAKKMGIPATAAFHTQPENVTWGIGVGPAGTLLADCIYYRFRRRMYRRVSRVHCPTEF
                     IAGELKRHNYRNILHVISNGIGPAFHLEETEKSKGKFEILSIGRYAPEKRQEIILKAI
                     FVSGHKNDIHLTLAGTGPKENKLRKIARKLDVDTTFDFYSTSDLIKVIHKSDLYVHAA
                     EAEIEGISCLEAFASGVTPVIATAKKSATAQFALDDRSLFKGGNYKELAKKIDYWIEN
                     PDELEKADHLYAESAEKYNLQLSCDLFEEMLKQAVEDKIRENAASTEAGRKISKRVKF
                     SPLKKVLSAAFYYLIAVPLLSIYIYFVRGVRIRNRRQLRKIKGGAVLISNHVHTLDSA
                     MNGIAAFPRKPVFTGLRANFELPVAGFFVNILGTVPVPETYDETKIFFYELTKQLRKG
                     KLVHFFPEGELIKYDTDLRPFKKGAFHIAEEAEVPIVPIGISFHEKTSIFPLLSPNKV
                     VLTVGDPIYPDAFKRKREAIDSLFEESQDAMEKLIKA"
     gene            complement(5268..6380)
                     /locus_tag="PLP02_05415"
     CDS             complement(5268..6380)
                     /locus_tag="PLP02_05415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPF42913.1"
                     /translation="MDIFLRIDTNLLAMAVLTFIIIMSINRLDKKTTMNRLFVLVCLL
                     DMTELVVETASCFLNGVPGTFVYVLSNILHVVLFSVAPLLGLHWFFLVRNIFKPYRRT
                     NKTLFFIMLLPVIVNFIVVLLSPFYGFTFTIGDGNVYSRGFWYYVTGGITFLYMLVSI
                     VIIIANHKNVLKDEFMMIMSIFMLPVLGGIMQAIFYGALLIWSSSAMSLVIAFIFLQQ
                     RMIHLDSLTGCWTRDSLLYFISLRLNRKDGGFGAIYFDIDKLKQINDSYGHLEGDNAI
                     KKAVSFVKMELGPGDIIARMGGDEFVVIVDSGSPEKISEILARIKENFADYNRRMENK
                     YVLDCSFGADVYSPNYVNFEQFLNNVDTLMYQHKER"
     gene            complement(6511..>7015)
                     /locus_tag="PLP02_05420"
     CDS             complement(6511..>7015)
                     /locus_tag="PLP02_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPF42914.1"
                     /translation="ADKNGETIVKLLLKSGKPLVIDADGLGYFKSQLKNAGLKQKIVI
                     TPHSGELMRLLDKSREEIYGDVDKSLEELTKRQITVILKGPSTFIAGGGRMACSVSGN
                     PGMASAGTGDVLAGIVAAMMARLEDPFEAAVKATIIHSEAGRKAKEVRGESGLMAHDL
                     VDYLPLP"
BASE COUNT         1860 a         1615 c         1487 g         2053 t
ORIGIN      
        1 tcgccaagcc gatgatcccg gcgccagcta gcaaagtagc tgtctgaacg ttccaagctg
       61 acaaaatcaa gcaaattgcc acgatgtagc tcagatactt gacgacgctt ttcaataaat
      121 taccgatcgt ttccgaacgg tggccttttt tggccaggat attgacaagc caagccagga
      181 ccttgttaag aacccaaacg aaaacgatga ccgccacaca ctcgagcaat gttatgtagt
      241 tgttggagaa aaaggtgatc aaatcgaaaa atttcccaag actgtttgcg atgatgttgg
      301 caaacacggt attcggaaaa agtcgaggtg cgaacattcc taagaacaga catatgaaga
      361 tcacagcgac tacaatgatt gtcttgacct tttccttaga cggtttgttt ttaaaattct
      421 caataatttc ttttatattc attttattgt ctccttccct cagggagtta tttccgttac
      481 gatgtaatag tagtaatact cggtgtccga actgatgccg atctcgatgt gccaatcttc
      541 gtaatccggc ttgtttatgg taaacataac aatcttggaa tgggcattga cggtgaaatc
      601 ggaatcacgc cagaattcga gttggtcata aatatagcca tccccataat cgactgtttg
      661 gtatattccg taataagcgt tttggaaagt gtcagaacta tcaacgattg tcacggtgac
      721 aagataggca tcgatagttt cggtcacatc gacattgcag tcgaaatcct gcatggtagt
      781 tacgctttct tcgtatacga cccgttcgac ataagccagt gtcgtaggat cttgataata
      841 agcgaacagt tttatgtcat atctccggtt atggcgcaag ttactgaaga cgatttcttc
      901 tccgcattcg tgcatataac cttcttccat tgatgacgga gtaatctcaa tcacctggag
      961 atccaccttg ttatgctgca aatggagttc gtaacggata tcttcgatgc catatggttc
     1021 ggcatatgcg ataacagaaa tcatgtcatt agttacttgt tcgatataaa atgatcccat
     1081 gtaatataac ggctcgtcaa aagttagttg atcaaatatt gtcttttctc cgtttacata
     1141 ttccccagaa atttcgatat atacagaact gttatcgtag taagggacaa ggatggacac
     1201 actggtttcg tttggcaaca gttgaaccgt cgtttcttcc tctacaatgt attccttgtc
     1261 catcagaacg taataagtca tagtgaatcc ggtcaattcg gtttccggat cggcaaggaa
     1321 gaatccgatg tcgtattcca gataactggt ttcgccagaa ctgatcagtt cgtaaccggt
     1381 gatagccccg ccggctctcg attcggtttt cactttgtcg ttcacaaggt attcggtgcc
     1441 gaatcccttg tccgcttcca ctctgatcga atattccgta ttagtcttca gtccggtgaa
     1501 tattcccgaa ctggttccca gttcaaggga ttgttcgtaa tattcgtcct ggcattccag
     1561 aaccactttc agagtcccgg caataattgc attttcctca tcggtgacat cgacatggta
     1621 gataatttcg ttagcatatg gttcgatggc cgcaaacgac gccacagcgc ttttaggaat
     1681 gaccgttgcg gcaatcacta ctgccgccac ggtaacggta agtgacgaaa aaatcgattt
     1741 gaacgcttca aatcgagttt ttttcttgat cttttcgcgg aactcaacca tccttgccaa
     1801 gtcgccaccc ccgcacttct ttattatttt acaaaacatt tatccgttaa gcaatatgtt
     1861 cttttttttt gtttgcatat tttgcttttt atccctgaaa taaacaaaaa accgtctttc
     1921 ggacggtctt tttatctctg gtcggaaaga caggatttga acctgcgacc tcttggcccc
     1981 cagccaagcg ctctaccaaa ctaagctact tcccgggcaa catatatttt accaaatatt
     2041 gccggaaaag ggaaatgttt tttcctgaaa aggcaaaata tgttcgttgg ggattattac
     2101 ttgactatga tgtctccctt gccaatcttc taacgtactt tttcatatac ttagtacggg
     2161 atttagcaat cctttttgct gttttagtat tcatgagatt cacgagcaga aacaatttct
     2221 gataaaaatg ggcaaaagag gaatcatcat cgactctgcc agagtatatt tttcttccca
     2281 gatgtccccc aaatgagaaa gtgcgggcga ttccaatcgc tcccaaggcg tctagtctat
     2341 cggcatcttg agcgattgcg gcttccttcg aagtgatttt tattccgttt ttttgcccgg
     2401 aaaaagagat ttcggaaata gcttttagta ttttagtttt gatcacggaa tcgaggtcgc
     2461 aggaatctag gtattttttg gccaagtttg tttcttttct ctgaagtttg ggatcatcaa
     2521 gatcatggag ataagcgacc atcaaggtga tgaaaggatc ggccggttcc tttgccagga
     2581 tttttcttgc attgccaacc acacgttcga tatgggaaaa gtcatgtccc ggttcgtcgt
     2641 tttccataat tgttttgaca tatgctgcgg cattttccaa aagaccgcat tgtttttggt
     2701 ctagtgatat atcagtcata ttcaacagcc attttcatat attctaaaga gttttcataa
     2761 tttgtttcta tttcttctgc cgtcagactc cgtttgacaa tcattggtac gcccagcgca
     2821 agcatccctt ctggaacagt ttttccctgc ggtacaagtg ccatggcagc gaccatcgca
     2881 tttttttcaa tgacggctcc gtctaaaatt acggctccca taccgacaag aacattgtcg
     2941 ccgatcgtgc atccgtgaat gatggctccg tgtccgatcg tgacgttatt cccgatcatg
     3001 gtcggatgat cgatgctcgt atgaacgatc gcattatcct gaatattcgt attcctgccg
     3061 atggttattt ttgccatgtc tccacgaacc acactaccaa accagacgct ggaattgtcg
     3121 ccgattttga catcgccgca aacggttgcg gaaggaaaca ctttcgcttt gccggatatt
     3181 gccggattaa tccctttata agaataaaca gccatatata cctcccaaaa aacccgtttt
     3241 cattatatcc gctatattag cgggaaacaa gaggtggaag aaaagaaaaa ccgcttacgc
     3301 ggcttttcag gcttttatga gtttctccat ggcatcctgt gattcttcga acagcgaatc
     3361 aatcgcttct cttttgcgtt tgaaggcatc cggatatatc ggatcaccga cggtcaggac
     3421 gactttattg ggagaaagca atgggaatat cgatgttttc tcgtgaaagc tgatgccaat
     3481 cggaacgatg ggaacttccg cttcttcggc gatatggaaa gcgcctttct taaaaggacg
     3541 gagatctgta tcgtacttga tcagttcgcc ttccggaaag aaatggacca atttcccttt
     3601 ccgaagctgt tttgttaatt cataaaagaa tatttttgtt tcgtcatatg tttcgggcac
     3661 cggaacagtt ccgaggatat tcacgaagaa gccggcaacc ggtaattcga aattggctct
     3721 taagccagtg aaaaccggtt ttctcggaaa agcggcaata ccattcatgg ccgagtccaa
     3781 agtatggaca tgattgctga tgagaaccgc tccgcctttt atctttctta actgtcggcg
     3841 attacgaatc cgaacgcctc tcacgaaata tatatatatc gacaaaagag gaacggcaat
     3901 aagataatag aatgcggcgc tgagtacttt tttcaacggg ctgaatttca cgcgtttcga
     3961 aatctttctt ccggcttccg tactggcggc attctcacga attttatcct cgaccgcttg
     4021 tttcaacatt tcttcgaaca aatcgcagga aagctgcaga ttatattttt cggctgactc
     4081 ggcatataaa tgatcagcct tttccaattc atctggattc tcgatccaat aatcgatttt
     4141 ttttgccagt tccttgtaat tcccgccttt gaaaaggctc ctgtcgtcca aagcaaactg
     4201 tgctgtagcg ctttttttag cggttgcgat cacgggagtc accccgctag caaaagcttc
     4261 aaggcaggat atgccctcga tctccgcttc ggcagcgtgg acataaagat ccgatttatg
     4321 gattaccttg ataagatcag atgtcgaata aaaatcaaat gtggtgtcga catccagttt
     4381 cctggctatt tttcttagtt tgttttcttt aggaccggta ccggccaaag taagatggat
     4441 gtcatttttg tgaccggata caaaaatcgc cttcagaatg atttcctgac gcttttccgg
     4501 agcgtagcgc ccgatggaaa gaatttcgaa tttccctttg gatttttcgg tttcttccaa
     4561 atggaaagcc ggaccgatac cgttcgatat cacatgcagg atattccggt aattatgtct
     4621 tttcaattct ccagcgatga attcggtcgg acaatgcaca cgcgaaaccc ttctatacat
     4681 ccgtcttctg aaccggtaat atatgcaatc tgccaacaac gttcccgccg ggcccactcc
     4741 gattccccac gtgacattct ccggttgggt atggaaagcg gccgttgccg gaatgcccat
     4801 ttttttggcg agtttgatac ctttgatcga tacggaaaac ggtaaaaaga aatggacgat
     4861 gtcggcgccc tcaaaggcct tttccatgac ttttcgatcg aatttggcaa aatacatatg
     4921 ctgtttcgcg gacacttccg agacaatcgg aatccaacgt ttcttcaatt ggtaatatcc
     4981 ttctccgatg atcggaccgg cggatacgat tctaacagta tggcctcttt tttccaacat
     5041 ttccttggtt cgttttgccg tcatcgtggt tccgttggaa agttctccgt aattgtcgat
     5101 gacgaatact atgatcatat gataacctcc cccgggtttt agcacctcta ttatatgcat
     5161 acatataata aaaagcaacc aaaataataa ttataatttg gccgctgtat actaaacaat
     5221 tgtatattat tagactctcc gccatatccg gcggtatttt gtccgattta tcgttctttg
     5281 tgctgataca tcaaagtatc gacgttattg agaaactgtt cgaaattcac ataattcgga
     5341 gaatagacat cggcgccgaa actgcagtca agaacatatt tgttctccat ccggcgattg
     5401 taatcggcaa aattctcctt gatcctcgcc aaaatttcgg aaatcttttc cggtgatccc
     5461 gaatcgacga tcacgacgaa ctcgtcgccg cccatcctgg cgatgatgtc gccgggtccg
     5521 agttccattt tgacgaaaga caccgccttc ttgatggcgt tgtctccctc cagatgcccg
     5581 tatgaatcat tgatctgttt cagtttgtcg atatcgaaat agatggctcc gaatccgccg
     5641 tctttccgat taagcctcaa cgatatgaaa taaaggaggg aatcccttgt ccagcagccg
     5701 gtcaggctgt ccagatggat catccgctgt tgcaagaaga tgaaggcgat gaccaggctc
     5761 atggccgagc tcgaccatat cagcaaggct ccgtaaaata tcgcctgcat tattccgccc
     5821 agcaccggca gcatgaagat gctcatgatc atcatgaatt cgtctttaag gacgtttttg
     5881 tgattggcga tgatgatgac gattgaaacc agcatgtaaa ggaaggtgat gcctccggtc
     5941 acatagtacc agaatccgcg gctatagaca tttccgtcgc cgatcgtgaa agtgaaaccg
     6001 taaaacggcg aaagcaagac aacgatgaaa ttgacgataa caggcaaaag cataatgaaa
     6061 aacagcgttt tattggttct tcggtaaggc ttgaaaatgt tcctgaccag gaaaaaccag
     6121 tgcaagccga gaagcggggc gaccgaaaac aggacaacgt gcagtatatt cgacagcacg
     6181 tagacgaaag tgccggggac gccgtttaag aaacacgagg cagtctcgac aaccagttcg
     6241 gtcatatcga gaagacatac cagaacgaac agacggttca tcgtggtttt tttatccaaa
     6301 cggtttatcg acattataat aatgaatgtc agaacggcca tagccagcag gtttgtgtcg
     6361 attctcagaa aaatatccat caaaacacca acttttgtcg tttggcgacc gcttaagaaa
     6421 agcaatcgca atatagtttt ttagatgagt accaaacagt attatatcat attatcaaga
     6481 aataagaaga acataaattt ttagtaaaaa tcacggaagc ggcaaataat caacgagatc
     6541 atgtgccatc aatccggatt cgccgcgaac ttcctttgct tttctcccgg cctccgaatg
     6601 aattatcgtt gcctttacgg ccgcttcgaa cgggtcttcc aatcttgcca tcattgctgc
     6661 aacgattccg gctaaaacat cgccggtccc ggcgcttgcc atgcccgggt tcccggaaac
     6721 ggaacaagcc atccgtccgc cgccggcgat gaatgtggaa ggccctttta aaatgaccgt
     6781 gatctgtcgt tttgtgagtt cttcgaggga tttatcgaca tcgccgtaga tttcctccct
     6841 gcttttatcc aaaaggcgca taagttcgcc cgaatgcgga gtgatgacga ttttctgctt
     6901 caatccggca tttttcaact ggctcttgaa ataaccgagc ccgtcggcgt cgatcaccag
     6961 cggttttccg ctttttaaaa gcagtttaac gattgtttct ccgtttttgt cagct
//
LOCUS       DAOPJL010000044         6351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_13092_length_6351_cov_7.887865, whole genome shotgun sequence.
ACCESSION   DAOPJL010000044 DAOPJL010000000
VERSION     DAOPJL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13092_length_6351_cov_7.887865"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PLP02_05425"
     CDS             complement(<1..1295)
                     /locus_tag="PLP02_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPF42915.1"
                     /translation="MNEKMILGVREKPKAGKWFALSFQHVFAMFGATILVPLLTGLPV
                     SVALFASGAGTLIYILCTNAKVPVYLGSSFAYITVVATVSGYSSDPLTANYAAALTGL
                     FTVGIVYVVFSLLIRFAGKKWIETILPPVIVGPMIMIIGLGLSSVAIANAGFNGGSWK
                     EILTAIVSLAIVIVVSLKGKGFIKIIPFLIGIVGGYIFASCVGLVDFSAFVAVITTPS
                     EWIRIPEFMFLGFFDGSANVLGTDITFYKANFAAVLTIAPLAFVTAAEHIGDHAVLSK
                     IVGKDFLQDPGLDRTLLGDGLATSFAALIGGPANTTYGENTSVVGMTRVASVYVTGGA
                     AVIAIVLSFFNVFTTLISTIPAPVMGGISIILYGFIASNGLKVLIDNKVNMFDLRNLI
                     IVATMLVIGLGGSMISFGTFQIYGMSLAALVGIVLNLTLP"
     gene            complement(1352..2767)
                     /locus_tag="PLP02_05430"
     CDS             complement(1352..2767)
                     /locus_tag="PLP02_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPF42916.1"
                     /translation="MKSFALKLKKYFKIDERGSSIGNELLGGLTIFLAMVYILPVNSS
                     ILSATGMSTGAVFAATALAAAAATLMMGLFANYPIGLASGMGVNAFFTYTIVIGLNYS
                     WQEALAAVLVSGILFLTISLTGLRKKVINAIPKNLKIAVGAGIGFFIAFIGLKDAGII
                     VGSAATLVTFGELNHPTILLALFGIILVFVLHSLHTKISRFAVIISIVVTGLLGILIG
                     IIYPPFASMMPSFSNASLGSISDIKYTFGQAFLALPDLLSNPMSYAVIFSLLFVDFFD
                     TAGTLMAVGHDAGLITKEGELIDGDKALISDATGTIVGAIFGTSNVTSYIESTTGIKQ
                     GARTGLSATVVGILFIVSLLFYPIFSFVGSIGVTLSDGTTMIFVSPATSMALVYVGAL
                     MASQLKEIDWSDAVASVTAFIVIIMMILSYSISEGIAFGFMFYVILMLASKRGKEVNS
                     VMYVLAGLFVVYYVLKFTVLM"
     regulatory      complement(2875..2976)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            3131..3436
                     /locus_tag="PLP02_05435"
     CDS             3131..3436
                     /locus_tag="PLP02_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HPF42917.1"
                     /translation="MKDSYFDGGLLQLIGWRILGFLVTVVTLGICFPWAVVMIQNWEV
                     KHTVINGKRLAFDGTAMQLFGNWIKWLLLGIITLGIYFLWVDIKMKKWVTKHIHFAQ"
     gene            3607..4119
                     /locus_tag="PLP02_05440"
     CDS             3607..4119
                     /locus_tag="PLP02_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPF42918.1"
                     /translation="MTDKRKKELWRAFKFLLFSASAGLIEFGSFTLLNEVKWFDFLDR
                     LSSFQYLGTEYTWHYAFCYLIALILSVLWNFTFNRRFTFKSANNINIAMLKVFVFYLF
                     FTPASTLITYLLADQMGLNEYVVLIIVMISNLLLEFPYQRFFVFGKSLDTNAAAIKAL
                     ENQETSISEN"
     gene            4292..4822
                     /locus_tag="PLP02_05445"
     CDS             4292..4822
                     /locus_tag="PLP02_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HPF42919.1"
                     /translation="MKDDKIDLIQKLLRKNELKAVENDEQAMHAILEKKISENINTLH
                     EGRLTVGQKVSDKVADWAGSWAFIISFVAILIAWIVINGIFLASGAFDPYPFILLNLI
                     LSCVAALQAPIIMMSQNRQDAKDRLRSENDYKVNLKAEIILEDLHKKIDTLLINQNDL
                     KKQIAELKKNGKQNNG"
     gene            complement(4866..6131)
                     /locus_tag="PLP02_05450"
     CDS             complement(4866..6131)
                     /locus_tag="PLP02_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42920.1"
                     /translation="MKISDVVYKTIEEVDQMQRFDMFMKPRKAPWYLQLLAWLLSFPE
                     TFSHRAKISKINMKGLKPPYIMLCNHNSFLDFKVATRAVFPHSSNYVVAIDGFINREK
                     LLRDVGCIGKRKFISDMPVVRNIQYSLKNLGQICQIYPEARYSLVGTTAILPESLGKM
                     AKLLKYPVVVLLSHGHHLSQPVWNLDKRKVPTRSEMTQIITQEEIRSLDVTEINRRIN
                     DAFVYDDYRYQLDNKIKIKTGNRAEKLHHVLYKCPYCGKEFQMQSEGNKIWCENCHTL
                     HEMDEYGRLENMGGETKFTHIPDWFEWEREEVKKEIMEGIYDVTLPVDVDSLPNATGY
                     YRLGRGLLTHNKDGFHLVVKQENKVLDVVKPVQENYSVHVEYDYFGKGCGLSFSTFDD
                     TYYIYPVDQKYSVTKFHFAVEELYKLQNK"
BASE COUNT         1796 a         1406 c         1499 g         1650 t
ORIGIN      
        1 ggcagtgtca gattcaagac gatgccgacc agggcggcca aactcatacc atagatctgg
       61 aaggttccga atgatatcat cgatccgccg aggccgatca caagcatggt ggcgacaatg
      121 atcagatttc taagatcgaa catgttgacc ttgttgtcga tcaaaacctt gagaccgttc
      181 gatgcgatga aaccgtaaag aatgatggag attccgccca tcaccggagc ggggatggtc
      241 gaaatcaggg tcgtaaaaac attgaagaaa gacaagacga tcgcgatcac cgcggcgccg
      301 ccggtcacat aaacggatgc gactctagtc atgccgacaa ccgaagtgtt ttccccgtat
      361 gtcgtattgg cgggtccgcc gatcaaagcg gcgaatgaag tggcgagccc gtcacccaga
      421 agcgtcctat ccaaaccggg atcctgcaaa aagtcttttc cgacgatctt gctcaaaact
      481 gcatggtcac cgatatgttc ggccgctgtc acgaaagcca aaggcgcaat cgtcaacaca
      541 gccgcgaagt tggctttgta gaaggtgatg tcggtgccaa gaacgtttgc gcttccgtcg
      601 aagaagccaa ggaacatgaa ctcgggtatc ctgatccatt cggaaggagt cgtgataacc
      661 gccacgaaag cggagaaatc gacgagtccg acacaggaag cgaaaatata accgccgacg
      721 ataccgatca ggaagggtat tatcttgatg aatccttttc ctttgaggga aaccacaatg
      781 acgatcgcaa gcgacacgat agcagtcagg atttctttcc agctaccacc gttgaaaccg
      841 gcattagcga ttgcgacgct ggaaagacct aatccgatga tcatgatcat cggaccgacg
      901 atgactggag gtaatatggt ttcaatccac tttttgccgg caaatctaat caaaagggag
      961 aaaacgacat aaactattcc gactgtgaag agtccggtca gagccgctgc gtaattcgcc
     1021 gtcaaaggat ctgacgaata gccgctcacg gttgcgacta cggtaatgta ggcaaatgaa
     1081 ctgcccagat ataccggcac tttggcgttt gtgcaaagaa tgtaaatgag ggttccggct
     1141 ccggaagcga aaagggcgac ggagaccgga agaccggtca atagcggtac caggatggtg
     1201 gcgccgaaca tggcgaacac atgttggaaa ctgagggcga accattttcc ggctttcggt
     1261 ttttctctta ctcctaaaat cattttttca ttcattttct ttcctccttt ttattttaaa
     1321 aaaaaggaag gacgaagtcc ctccctttga atcacataag cacggtgaat ttcaaaacgt
     1381 aataaaccac aaacaatccg gctagaacat acattaccga attcacttcc ttgcctcgct
     1441 tcgaagcgag catcaggatg acatagaaca tgaaaccgaa tgcgatgcct tccgaaatcg
     1501 aataggatag gatcatcatg atgatgacga tgaaagccgt gacggaagcg acggcatcgg
     1561 accagtcgat ctctttaagt tgcgaagcca tcagggcacc gacatacacg agagccatcg
     1621 aagtggccgg agaaacgaag atcatcgtcg tgccatcgga caaagtgacg ccgatcgatc
     1681 cgacgaacga gaagatcgga tagaaaagca atgatacgat gaagaggatg ccaacgacgg
     1741 tggctgacaa acccgttctc gcgccctgtt tgattccggt ggtcgactcg atataggaag
     1801 tgacgttcga ggttccgaag atggcgccga cgatggtgcc ggtagcgtcg gagatcagag
     1861 ccttgtctcc gtcgattagt tcgccttctt tggtgatcag tccggcgtca tggccgactg
     1921 ccatcaaagt tccggctgta tcgaagaagt caacgaacaa cagcgagaag attactgcat
     1981 aggacatcgg attggaaaga agatccggca aggcgaggaa agcctgtccg aaagtgtatt
     2041 taatgtcgga aatactacca aggctagcat tggagaatga aggcatcatg gaagcaaaag
     2101 gcgggtatat aattccgatg agaattccca acaaaccggt gacgacgatc gagatgatca
     2161 cggcgaaacg gctgattttc gtgtgcaggg aatgaaggac aaaaaccagg atgattccga
     2221 aaagggcaag caggatggtc ggatgattga gttcgccgaa cgtaaccaaa gtggcggcgc
     2281 ttccgacgat gatgccggcg tctttaagac cgatgaaggc aataaaaaaa ccaatgccgg
     2341 cgccaactgc tattttcagg tttttcggta tggcattgat cacttttttt cttagaccgg
     2401 tcagggaaat ggtcaggaac agaattccgg acacgagtac ggcagcgaga gcttcctgcc
     2461 aggagtagtt gaggcctatt acgatcgtgt aggtgaaaaa ggcattgaca cccattccgg
     2521 atgccagacc gatgggatag ttcgcaaaaa gtcccatcat gagagtggct gcggctgccg
     2581 ccaaagccgt cgcggcgaat accgccccgg tgctcatccc ggtagctgac agaatcgatg
     2641 agttgacagg cagaatgtaa accatggcca gaaagatcgt caggccgcca agcagttcat
     2701 tgccgatcga ggaaccgcgt tcgtcgatct tgaaatactt cttcagtttc aaagcaaagc
     2761 ttttcattca ttttcctccc gttttatatt tttgccgaca aaagacaata taaaaaaacc
     2821 ttggatacgg tccaaggcaa acacaacaaa aattgaagga atttcgtcat ccaattcttc
     2881 gtatgtgtac gccgtagtcc tgccatttac ggtgacaggg tagaaacttt cgaaccatat
     2941 tatcggaatt atacgacttt tgtcaactac tctttgcatt atatcatgct tcttcgcgat
     3001 aaggcaatca tctttttgat gtaaacgttt cacaaattat tttcataact tctgaaaacg
     3061 actttatttt ctttgttata attgaatcat agataaaatt gttttacaaa aagggaggag
     3121 aaaacgaaaa atgaaagatt catattttga cggaggtttg cttcaactca tcggctggag
     3181 aatactggga tttttggtga cagtcgtcac tttgggaatt tgtttcccgt gggcggtggt
     3241 catgatccaa aactgggaag tcaaacacac ggtcataaac ggcaaacgtt tggctttcga
     3301 cggaaccgca atgcaactgt tcggcaactg gatcaaatgg ttgcttcttg gtatcatcac
     3361 gcttggcatc tattttttat gggtggatat caagatgaaa aaatgggtca ctaagcatat
     3421 ccatttcgca cagtaaaccc ggactcaaag ctccgcattc atgcggagcc ttttttattt
     3481 acgaatcgac agtattttat cgtcaagtat tgactttatc gggccgtgat gataaaaata
     3541 tcaaggcgta tgtatgagaa tttattttgt cttttaagta tttaatagct tatgggagga
     3601 ttaatggtga ctgacaaacg taagaaagaa ttgtggcggg ctttcaagtt cttgctgttt
     3661 tccgcttcgg cagggctgat cgaattcggc tccttcacgc tgctaaacga agttaaatgg
     3721 ttcgattttc tggatcggct tagttcattc caatatcttg gtacggaata tacctggcat
     3781 tatgcgtttt gttatttgat cgcattgatt ctttccgtct tgtggaattt cactttcaac
     3841 cggcggttca ctttcaaatc ggccaacaat atcaatatcg ccatgctcaa ggtatttgtt
     3901 ttctacttgt ttttcactcc ggcttcgact ctgataacct atctcttagc cgatcaaatg
     3961 ggtttgaacg aatatgtcgt gctaataatc gtcatgatct ctaatttgct tcttgagttt
     4021 ccctatcagc gcttctttgt gttcggcaag tcgctggata cgaacgccgc cgcgatcaaa
     4081 gccttggaaa atcaggaaac aagcatttcg gaaaactaac cttccattaa tgaatgaaga
     4141 ctaccaataa ctgaaaggcc cgtaatccgg aaatggatta tcgggctttt cttatctttg
     4201 acaaaatgtc gctttggctt ttactcataa aggaaaagtg ttatcattta agacggatat
     4261 caatataaat actatataag gagaattgga tatgaaagac gacaaaatcg atctcatcca
     4321 gaaattactc agaaagaacg aactgaaagc cgtggaaaac gacgaacagg ccatgcatgc
     4381 gattttggaa aaaaagattt ccgaaaacat caacacgctt catgaaggtc gtctgaccgt
     4441 cgggcagaag gtatcggaca aggtggccga ctgggccgga agttgggcgt tcatcatctc
     4501 ttttgtcgcc atactgatcg catggatcgt catcaatgga atcttcctcg ccagcggtgc
     4561 gttcgatccc tatcctttca ttcttctgaa tctcatattg tcttgcgtgg cggcacttca
     4621 agctcccatc atcatgatga gccagaaccg gcaggacgcc aaagaccgtc tgcgttcgga
     4681 gaacgattac aaagtcaatc tgaaggcgga gatcatttta gaggatctcc acaagaagat
     4741 cgacaccttg cttatcaacc agaacgatct caagaaacag atagccgaac ttaaaaaaaa
     4801 cgggaaacaa aacaacggat aggaaataaa aggctccgtt ccacgattgg gaacggagcc
     4861 ttctttcatt tgttttgcag tttgtaaagt tcctcgaccg caaaatggaa tttggtgacc
     4921 gaatacttct gatcgaccgg atagatataa taagtatcgt cgaaagtgga aaagctgaga
     4981 ccgcagcctt tgccgaaata atcgtattca acatggacgc tgtagttctc ctgaaccggt
     5041 tttacgacat ccaacacctt gttctcctgt ttgaccacca gatggaaacc atccttgttg
     5101 tgagtaagca agcctctgcc gagtcggtaa tagccggtgg cgtttggcaa gctgtcgaca
     5161 tcgactggca aagtcacatc gtaaatgcct tccattattt ctttttttac ttcttccctt
     5221 tcccattcga accaatcagg aatatgagtg aatttggttt cgccacccat gttttcgagc
     5281 cggccgtatt cgtccatctc atgcaaggta tggcagtttt cgcaccaaat cttgtttcct
     5341 tcgctttgca tctggaattc tttgccgcaa tatggacatt tatagaggac gtggtgaagt
     5401 ttttcggccc gattcccggt tttgattttt attttgttat ccaattgata gcggtaatcg
     5461 tcataaacga aagcatcgtt tatccgccgg ttgatttcgg tgacatcgag actgcggatc
     5521 tcttcctggg tgatgatctg tgtcatctca gatctggtcg gaactttccg tttatcaaga
     5581 ttccagaccg gctgactcag atggtggccg tggcttaaaa gaacgacgac cggatatttg
     5641 agtagcttag ccatttttcc gagactctcc ggcaaaatcg ccgtggtccc cactagcgaa
     5701 tacctggctt cgggatagat ctgacagatc tggcccaagt tttttaggga atactggata
     5761 ttcctgacga ccggcatgtc ggaaatgaat ttgcgtttgc cgatgcaacc gacatcgcgt
     5821 agcagttttt cccggttgat gaatccgtca atcgccacga cataattgga ggaatgcgga
     5881 aatacagccc tggtcgctac tttgaaatcg agaaaactgt tatgattgca aagcatgata
     5941 tagggaggtt tcaggccttt catgttgatt ttggatattt ttgccctgtg agaaaatgtt
     6001 tctggaaaac tcaaaagcca agccaatagc tgcaagtacc aaggagcttt ccgtggtttc
     6061 atgaacatat cgaatctttg catttggtct acttcttcga tcgttttgta cacaacatcg
     6121 gatattttca tttgcgcgcc cttccattat atgttgccaa tgataagata ttatcacatc
     6181 ttggcattaa aaagggggat tttttgctaa aaaaccggaa aaatcgtgcc gttttgccat
     6241 catattcatt aatgccagat ttgataaacc cggcacatat aaaaaaagcc cccggggaaa
     6301 gcgttcgatt aaaacagaca gaaaataaaa agagtcatat tggataatat t
//
LOCUS       DAOPJL010000045         6169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_13645_length_6169_cov_6.837095, whole genome shotgun sequence.
ACCESSION   DAOPJL010000045 DAOPJL010000000
VERSION     DAOPJL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13645_length_6169_cov_6.837095"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..2697
                     /gene="alaS"
                     /locus_tag="PLP02_05455"
     CDS             58..2697
                     /gene="alaS"
                     /locus_tag="PLP02_05455"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPF42921.1"
                     /translation="MKYMTGNEIRNTWLDFFKSKGHAVEKGASLIPNDDPTLLWMNAG
                     VAALKKYFDGSQVPKNPRIVNVQKCIRTNDIEHVGKTARHHTFFEMLGNFSIGDYFRK
                     EAVIWGYELLTDPKWFGFDKDKLYMTIYPADNETRDLWISLGVDPSHIIPTKEGNFWE
                     IGEGPCGPCTEIHFDRGPSYGDFGPEAIRDDIENDRYIEIWNIVFSQYNAIPGKKRSE
                     YPELPNKNIDTGCGLERMACVLQGAETNYETDLFMPIIRKIEEICGLDYKGQMAFKVI
                     ADHIRTVTFAVADGAVLSNEGRGYVLRRLLRRAVKYGRKIGIDRPFMADLVDIVIDIM
                     GEFYPYLKDRAGIIKKIVSQEETKFLETIALGEKRFEAIVSQSKGQKISGSDAFLLYD
                     TFGFPFELTMEYAEEIGYSVDKAGFEEEMEKQKERARNARKDLQSMKSQNEDYLEFYD
                     KSEFVGYENMQISTKIIKVFPDGIVLEKTPFYATSGGQVSDTGTIGNNDFSLNVTDVS
                     KLPNGQFIHAFDIVFGSPVEGAEVKAAIDEIRRKKIARHHSATHLLFKALRDILGNHV
                     SQQGSQVSEESLRFDFNHYEMPSDKEILEVEDKVRKMIFQSHKAHTDIVTVEEAVKQG
                     AIAEFGEKYGDSVRMVNLYYTLDLCGGTHVGDVADIGDFAIRSIYSIGSGIYRIEALA
                     DGEVARLKDAYANIDAELKNIRERIGEILENAKSEGLKIEYTEPSFPKLEGGYRDILA
                     KREEFRVMQEKIKDLDKDYQRLLYDKKAIDFAPYLKLAVNNRIVVEIKNASVDQLKQL
                     ADNLSNNLENGFVMLASIIEDGKIMFVAKTTNNNLHAGNIVKKAAQICGGNGGGRQDF
                     AQAGGKDKEKLSEALAAIRKELS"
     gene            2694..3110
                     /gene="ruvX"
                     /locus_tag="PLP02_05460"
     CDS             2694..3110
                     /gene="ruvX"
                     /locus_tag="PLP02_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPF42922.1"
                     /translation="MRYLGLDLGSKTLGIAMSDPSGIIANGITTFNFAEGNYGEAIEY
                     TLQLIISHHIGTVVIGHPKNMDGTCGFQARISENFRDELARRTSVPAVLWDERLTTKI
                     ANQTLYDACAGRREKKSKVDQLAATVLLQSYLDSRK"
     gene            3118..3429
                     /locus_tag="PLP02_05465"
     CDS             3118..3429
                     /locus_tag="PLP02_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPF42923.1"
                     /translation="MENDKDYLIVTDENGNELECEIIMTFASEEFGKSYVVYQIKGDE
                     EGKYFAASYNPEDDDEGKLFEIESDAEWDLIEEVLENFLEDDEEDDEEAEEETDIDEE
                     K"
     gene            3525..4334
                     /locus_tag="PLP02_05470"
     CDS             3525..4334
                     /locus_tag="PLP02_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPF42924.1"
                     /translation="MASLANSKEITRLFQAGADIVMLEVSGLCQAPLCPFSLDDIEKI
                     AASTHEQGKQIFVLADMTVTEDELDRVKKNMERLLSCKANRIVCYDFTFAILAAKAGM
                     ANRIIYAPNTLITHSRLANFYKKTGLAGMVVSESLSAAEILSVCKKSEGLEIGAVLSG
                     HREIYRSRRHHVTAYIAHNRIQNPTPEFSSGYAVMEKQRQNSFYPLIENTNGTVIIDA
                     GMSCPDWLTPELAEALTYGFISRRNIELTDYIEMLNKIKSTVLLQKAGDVS"
     gene            4331..5506
                     /locus_tag="PLP02_05475"
     CDS             4331..5506
                     /locus_tag="PLP02_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPF42925.1"
                     /translation="MKPELLAPAGDIEKARFALIYGADAVYLGAREFSLRARATGFSM
                     EEIGAITEFAHSLGKKVYIACNSILHDDDLPEFSAFLEKLATIPIDGVIASDPYVIEL
                     VLKTTAIQVHLSTQMSTTNSELCEFWQNQGVGRIVLARELGMKEIRTIREKVTSELEV
                     FVHGGMCSAYSGRCLLSETMTGRDANQGDCAHSCRWEYELYHGGELIFDDFLIAARDL
                     SSIRQIPELMEIGIDSLKIEGRMKSIHYVATITNAYRQLIDDIEKAVPIDFSKYEKMI
                     ARAENRFSGPAFLAGANPEIMQMHADDEKFPEQDFSAVVRGFQNGKTIVEQRNRFSVG
                     DTLELFSSKGLTGVFKIKTITDDVGNTLDVANHPMQKLYLDIPFSTNPYDILRRLDQ"
     gene            5506..6126
                     /gene="udk"
                     /locus_tag="PLP02_05480"
     CDS             5506..6126
                     /gene="udk"
                     /locus_tag="PLP02_05480"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017553152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPF42926.1"
                     /translation="MKNPVVIAVAGGSSSGKTTVVNSILQNLGNMPIVVIKHDDYYKD
                     QSDMTMEERRKVNYDHPFALDNDLFYDHLLQLIAGQTVEKPTYDFVNLTRAEKTEVIK
                     PAKIIILEGILVLEDERIRNLADIKIYVEADDDLRFIRRLLRDTKERGRTLESVVSQY
                     LSTVKPMHYAFVKPTKRYADIIIPNDYSHDVAVDLVKAKIISILSK"
BASE COUNT         1861 a         1366 c         1479 g         1463 t
ORIGIN      
        1 atcaatcgtc ctttacagat aaaggatgat tttctttttt tagtgtggag gatacatatg
       61 aaatacatga cgggcaatga aatcagaaac acctggttgg attttttcaa aagcaagggg
      121 catgccgtgg aaaagggagc ttccctgatt cccaatgacg atccgaccct tctttggatg
      181 aacgccggcg tagccgctct caaaaaatat tttgacggat cgcaggttcc caagaatccc
      241 agaatcgtca atgtccagaa atgtatccgg acaaacgata tcgaacatgt cggaaaaacc
      301 gcaaggcatc atactttttt cgagatgctc gggaattttt cgatcggcga ttatttccgt
      361 aaggaagccg tcatttgggg atatgaattg cttacggatc ccaaatggtt cggctttgac
      421 aaagataaac tgtacatgac gatatatccg gccgacaatg aaaccagaga tctttggatt
      481 tctctcggag ttgatccttc acacataatt ccgaccaagg aaggaaattt ctgggaaatc
      541 ggcgaaggcc cttgcgggcc gtgtaccgaa attcattttg accgtggccc aagttacggc
      601 gatttcggcc cggaggcgat cagggatgac atcgaaaatg accgttatat tgaaatctgg
      661 aacatagtgt tttcccaata caatgccata ccgggtaaaa aaagaagcga atatcccgaa
      721 ctcccgaaca aaaatatcga caccggatgc ggattagaac gaatggcatg cgttcttcag
      781 ggagccgaga cgaattacga aaccgatctt ttcatgccga taatcaggaa aatagaagaa
      841 atctgcggat tggattataa gggacaaatg gcttttaaag tcatcgccga tcatattcgt
      901 accgtcacat tcgccgttgc ggacggagcg gtgctttcca acgaaggtcg cggttacgtc
      961 ctaaggcgct tattgagacg cgctgtcaaa tacggacgca agatcggtat cgaccgcccg
     1021 ttcatggccg atcttgtcga tatcgtcatc gacatcatgg gggaattcta tccctatttg
     1081 aaagatcgcg ccggcataat caagaaaatt gtttcccagg aagaaaccaa gtttttggaa
     1141 acaatcgctc tcggagaaaa acgtttcgag gcaattgtct cccaatcaaa aggacaaaaa
     1201 atttccggtt ccgatgcttt tctgctttat gataccttcg gttttccgtt cgaactgact
     1261 atggaatacg ctgaagaaat cggttattcg gtcgataaag ccggtttcga agaggaaatg
     1321 gaaaaacaga aagaacgtgc tagaaacgcc agaaaagacc tgcaatcgat gaaatcccaa
     1381 aatgaggatt atctggaatt ttacgataaa tcggaatttg tcggatacga aaacatgcag
     1441 atttccacta aaatcatcaa ggtgtttcct gatggcatag tacttgaaaa aacgccgttt
     1501 tacgcgactt ccggcggtca ggtatcagac acgggaacaa tcggtaacaa tgatttttcc
     1561 ttaaatgtca ccgatgtttc gaaacttccg aacggtcagt tcattcatgc attcgacata
     1621 gtgttcggtt ctcccgtgga aggcgccgaa gttaaggccg cgattgatga aatcagacgc
     1681 aaaaaaattg ccaggcatca ttcagccact catttgcttt tcaaggctct ccgcgatatt
     1741 cttggtaacc atgtttccca acagggtagc caggtttccg aagaatcgct ccgtttcgat
     1801 ttcaatcatt acgaaatgcc ttctgataag gaaatccttg aagtcgaaga caaagtccga
     1861 aaaatgattt tccagtccca taaagcgcac accgacatcg ttacggttga ggaagccgtc
     1921 aaacagggag caattgccga attcggagaa aaatacgggg atagtgtcag gatggtcaat
     1981 ctttactata ctctagatct ctgcggcggc acgcatgtcg gcgatgttgc cgatatcggc
     2041 gattttgcga ttcgttccat ttattcgatc ggttccggca tataccggat cgaagcgttg
     2101 gcagacggag aagttgccag acttaaagat gcttacgcca atattgacgc ggaactgaaa
     2161 aacatcaggg aaagaatcgg ggaaattctt gaaaatgcta aaagcgaagg cttgaagatc
     2221 gaatacacag aaccgtcatt tccaaaactg gaaggcggtt acagagacat acttgccaaa
     2281 cgcgaagaat tccgtgtgat gcaggaaaaa atcaaagatc tcgataaaga ttaccagcgt
     2341 cttctttatg acaaaaaagc gatcgatttc gctccatatc tcaaattggc ggttaataac
     2401 cgaattgtcg tcgagatcaa aaacgcatcc gtcgatcaac tgaaacaact tgccgacaat
     2461 ttgtctaaca atctggaaaa tggttttgtc atgctggcat cgataatcga agacggcaag
     2521 atcatgtttg tggccaaaac cacaaacaat aatcttcatg ccggcaatat cgtcaagaaa
     2581 gccgcccaaa tctgtggcgg aaatggcggc ggacgtcaag attttgccca agccggcggc
     2641 aaggataagg aaaaattgtc cgaagctttg gctgcgataa ggaaggaact gtcatgagat
     2701 atctgggact tgatctaggg tcgaagactc tagggatcgc catgtccgat ccgtccggca
     2761 tcatcgcaaa cggcatcacg acattcaatt tcgccgaagg aaactacggc gaagcgatcg
     2821 aatacacatt gcaactcatc ataagtcacc atatcggaac cgtggtgatc ggtcatccca
     2881 aaaacatgga cggcacctgc ggattccaag cgaggatttc cgagaacttc agggacgaac
     2941 tggcaagaag aacctcggtc cctgccgtat tatgggacga gaggctgaca acgaagattg
     3001 ccaaccagac gttatacgac gcctgtgccg gcagaagaga aaaaaagagc aaggtcgacc
     3061 aattggccgc cactgtgctt ttgcaaagct atcttgatag cagaaagtag gtctgtcatg
     3121 gaaaacgaca aagattatct aatcgtaacc gacgaaaacg gcaatgaact ggaatgcgaa
     3181 atcatcatga ctttcgcatc cgaagaattc ggcaaatcgt atgtcgtcta tcagatcaaa
     3241 ggcgacgagg aaggcaaata tttcgccgcc agctataatc ccgaagatga tgacgaagga
     3301 aaactgttcg aaatcgaatc cgacgccgaa tgggatctga tcgaagaagt gctcgaaaac
     3361 ttccttgaag acgatgaaga agacgacgag gaagccgaag aggaaacaga catagacgaa
     3421 gaaaaatgac aggtacgcca atcatgaaat ggctgtgaaa atgcggccgc ttttggcgtt
     3481 agcggacctt tatcgagaag gcgtggtgaa cggatttgca aatagtggcc tcattggcaa
     3541 atagcaaaga gatcacccga ctattccagg cgggcgccga catcgtgatg ttggaagtca
     3601 gtggattgtg tcaggctcct ctttgcccgt tttcattgga tgatatcgaa aaaatagcag
     3661 ccagcactca tgaacaaggc aaacagattt tcgtacttgc ggacatgact gtcacggaag
     3721 acgagctcga ccgagtcaaa aagaacatgg aaaggctgtt gtcttgcaaa gccaaccgga
     3781 tcgtttgtta tgatttcacc tttgccatac ttgccgcaaa agcgggaatg gccaaccgga
     3841 ttatttatgc tcccaatact ctcataaccc attctagact agcgaatttc tacaaaaaaa
     3901 ccggtttggc gggcatggtg gtgtccgaaa gtttatccgc cgccgagata ctgtcggtgt
     3961 gtaagaaaag cgaagggctg gaaattggag cagttttgtc aggacatcgg gaaatctacc
     4021 gttcccggcg ccatcatgtg acggcttata tcgcccataa ccggattcaa aatccgactc
     4081 cggaattttc aagcggatac gcagtcatgg aaaaacaacg gcaaaacagt ttttatccct
     4141 tgatagaaaa cacaaacgga accgtgataa tcgatgccgg gatgtcgtgc ccggactggc
     4201 tgacgcccga attggcggag gccctcacat acggatttat ttcaagacgg aatatcgaat
     4261 tgaccgatta tatcgaaatg ttaaacaaaa tcaaatcgac agtgctttta caaaaggccg
     4321 gtgatgtatc atgaaaccgg aattgctggc tccggccggc gatatcgaaa aagcccgttt
     4381 cgctttgatt tacggcgccg atgccgttta tttgggagcc agggaatttt ccctgagagc
     4441 cagggcgacc ggattttcga tggaagaaat cggcgcaatc accgaatttg cccactcgct
     4501 tggaaaaaaa gtgtacatcg cttgtaacag tatcttgcat gatgacgact tgccggaatt
     4561 ttccgctttt cttgaaaaac ttgcgacaat tcccatagac ggcgtgatcg catccgaccc
     4621 gtacgtcatc gagcttgttc tgaaaacgac cgctatccag gtccatcttt cgacccagat
     4681 gtcgactacc aacagcgaac tatgcgaatt ttggcagaat cagggagtcg gacggatcgt
     4741 gctcgccaga gagttgggta tgaaagaaat acgcacgatc agggaaaaag tgacatcgga
     4801 acttgaagtg tttgtccacg gcggcatgtg ttcggcttat tcaggacgct gtctgctttc
     4861 ggaaacgatg accggaaggg atgccaatca aggagattgc gcccacagtt gtcgctggga
     4921 atatgaattg tatcatggtg gagaattaat attcgatgat tttctgatcg ccgccagaga
     4981 tctgtcgtcg atcagacaga tacccgaact tatggaaatc ggcatcgatt cgctgaaaat
     5041 agaaggacgg atgaaatcca tccattatgt cgccaccatt acaaacgcct atcgtcaatt
     5101 gatcgatgat attgaaaaag ccgtaccgat cgatttttcc aaatatgaaa agatgatcgc
     5161 cagggcggaa aaccgctttt caggcccggc attccttgcc ggagcaaatc cggaaatcat
     5221 gcagatgcat gcggatgacg aaaaatttcc ggaacaggat ttttccgctg tggttcgcgg
     5281 attccaaaac ggaaaaacaa tcgtggaaca gagaaaccgt ttctcagtcg gagacacctt
     5341 ggaacttttt tccagtaaag gattaaccgg tgtgttcaaa ataaagacaa tcaccgatga
     5401 cgttggcaac accctggatg ttgccaacca tccgatgcag aaattgtatc tggatattcc
     5461 gttttcgaca aatccctatg atattctcag gaggttggat caatgatgaa aaacccggtt
     5521 gtcattgccg tggccggagg ctcgtcctcc ggaaaaacaa cagtcgtcaa cagcattctt
     5581 caaaatcttg gcaatatgcc gatcgtcgtc atcaagcatg atgattatta caaagatcag
     5641 tcagacatga cgatggagga aagaagaaag gtcaactatg accatccttt cgccctagac
     5701 aacgatcttt tctatgatca tcttttgcag ctgatcgccg gacaaacggt tgaaaaaccg
     5761 acatatgact ttgtgaatct gactagggcg gaaaaaacgg aagtgatcaa gccggcgaag
     5821 atcatcatct tggaaggcat tttggttctt gaagatgaac ggatcagaaa cctggccgac
     5881 atcaagatct atgtcgaagc tgatgacgat cttcgcttca tcagacgact gcttcgggac
     5941 acaaaggaac gcgggcgcac tctggaaagc gtcgtcagcc aatacttatc gaccgtcaaa
     6001 ccgatgcatt acgcttttgt gaaaccgacc aaacgttatg cggatatcat catcccgaat
     6061 gattattccc atgacgtcgc cgtcgatttg gtaaaagcta aaataatatc tatcttaagc
     6121 aaatgaatta tgctataata aaaatcactt aatatttagg agagcggat
//
LOCUS       DAOPJL010000046         5970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_14293_length_5970_cov_5.049535, whole genome shotgun sequence.
ACCESSION   DAOPJL010000046 DAOPJL010000000
VERSION     DAOPJL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14293_length_5970_cov_5.049535"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLP02_05485"
     CDS             <1..452
                     /locus_tag="PLP02_05485"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF42927.1"
                     /translation="ATNFLAHSYLDYIWNTAAVRKEYLALVEGHLAGKAGRIELPIGN
                     DRHRNNHYIVYSNGKPAITDYEVIKEYPGYSLLRISIETGRTHQIRVHMGHIGHPVLG
                     DLAYGARHFACDRVQLHASSITYPHPLTHEKIIVTSPLPEDFRQYAY"
     gene            496..1920
                     /gene="pyk"
                     /locus_tag="PLP02_05490"
     CDS             496..1920
                     /gene="pyk"
                     /locus_tag="PLP02_05490"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPF42928.1"
                     /translation="MKRKTKIVCTIGPAVDSEEMIGKLIEAGMNVGRMNFSHGTHEEH
                     LRRLNMVRSVAGRMNKYVGAMADTKGPEIRTGDFQNGQAFFPKGSTVEIVKEEILGDD
                     KHFHISVPELFDDMKAGDYLLIDDGKVRLTVTEKRVGSLVCVVNNAGVIKSKKGVNVP
                     NVKLSMPFISEKDKDDIRFASENKFDMIAVSFCRRKQDILDVRALLEQNGCGKMEVIA
                     KIENQEGVDNLSEILEVVDGIMVARGDLGVEVSTALVPIYQKKIIRMANEVGKPVITA
                     TQMLESMISWPRPTRAEASDVANAILDGSDAIMLSGETAAGEYPIEAVQTMDTIAQAI
                     EEIIPYDERLQRSIKTSQSTVNDAIGIAVSQTVLSLPSAEVVVAFTETGGTAKRMCKF
                     RPEVPIIAITDNVETCRKLSYYWGVIPVLKKDVTDYSHYDLYAIEAAIGCGYKPGTTI
                     VMTSGWGQEHGQTNTMRIITIPNI"
     gene            complement(1982..2680)
                     /locus_tag="PLP02_05495"
     CDS             complement(1982..2680)
                     /locus_tag="PLP02_05495"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPF42929.1"
                     /translation="MNWKNFTRVLMPFVLVCLMQFFTYWGNQWYAEANGVVGIDLSVI
                     FPNFNQAVPFWSYSIYPYVLSYPFWVFTFFYVGYRSKHNMYLLVSLVLVTFTVCGLVY
                     FFYQTDVEMWRVSSGLFSIQDPNFTERLVLKIYASAGPRNAMPSMHCLMSWLCIIGAR
                     IDKKMPLPAKIFIWVMGITICISTQTLKQHYIIDLITGVALAEAAYWIFRKSSLTGKL
                     ESFFTKLNIKMKLE"
     gene            2840..3706
                     /locus_tag="PLP02_05500"
     CDS             2840..3706
                     /locus_tag="PLP02_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase"
                     /protein_id="HPF42930.1"
                     /translation="MFGLRNDGKRIKTIDPVMKMVPHIMKTRNDAQVMALYEIDCKGL
                     DEYIFAKRHEENIRFTYMHILIAAFVRVMALRPKLNRFIVNGRVFKRNEIQISFAVKK
                     TLLDTAEETTIKLTFDGTESIYDVKKKIDDAVLENAKESAVNGTDKMAKLLTIVPNGL
                     IKILVGFLMWLDKHGLLPKKVLEVSPFHTSLFLTNMKSIKMGYVYHHCYNFGSTSIFV
                     SMGKEKYEPVILDADDAKFGVAKIMKVGVVVDERICDGLYYGNSLREFKKYMEYPTVL
                     EERIDHKIEDQK"
     gene            3735..3974
                     /locus_tag="PLP02_05505"
     CDS             3735..3974
                     /locus_tag="PLP02_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42931.1"
                     /translation="MDLFMKILGICIGLFFLFIGIRFAFRSTNVINGIQRYKYHETQT
                     PRKQELLFARIIGVLLILVGLYFTMIAVLSLFPDS"
     gene            4087..5919
                     /locus_tag="PLP02_05510"
     CDS             4087..5919
                     /locus_tag="PLP02_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPF42932.1"
                     /translation="MNIIRLNDIEKTFGGTLLFKHVSFEVNDDDKVAVIGKNGVGKTT
                     LFKIMLGAISPDSGEIFISRQARIGYLSQDVLEPGERTLFGEMLLLYQELENLGQNLD
                     QAAKALATDHSDKAMERYSRLETEFQIKGGYQYKTDIETMLTRFGFSREDHDRFVSGF
                     SGGEKTRIAFAKLLLMHPDILLLDEPTNHMDIEIIEWLEDYLKKYQGAVVVVTHDQYF
                     INKVTHIIYELDQETMNRYTGTYEEYEAEKMKRYELLMKQYVRQQKEIAHLQSYVDRF
                     RYKANKASQAQDRIKKIAKIDRIDKPTKSEREISFAFRTPRPTDAIILEAKNLTIGYN
                     KPLIENISFQMRGRDKIGIIGQNGAGKTTLVKTLLGTISALKGNIAFPKQLKIGYFDQ
                     MATENRMKGKLIDIVHDIYPQKNLLEVRKILARFQFVGEDVFKETEMLSGGEMVRLRL
                     LLLLLEAPEFLVLDEPTNHLDISTKDIVEDVFAEYDGPIVFISHDRYFINKVATRIIE
                     FDETKWQLYEGNYDFYKESKGNNIPTAEKKEKKPRTVDPEKEIRKLESSLEENSVRIS
                     VLQKSLFQPEIYMDKSKYTETETEIKLLETTTINIMKRIEELSS"
BASE COUNT         1857 a         1294 c         1381 g         1438 t
ORIGIN      
        1 ttgctaccaa ttttctcgcc cacagttatc tggattacat ctggaatact gcggctgttc
       61 gcaaggaata cctggctcta gtcgaaggac acctcgccgg caaagcggga agaatcgaat
      121 tgccgatcgg caacgaccgg catcgaaaca accactatat cgtctatagc aacggcaaac
      181 ccgcgattac cgattatgaa gtgatcaagg aatatcccgg atactcgctt ctcaggatat
      241 ctattgaaac cggaagaacc caccaaatac gcgttcatat gggacacatc ggtcacccgg
      301 ttttgggaga cttggcttat ggcgcaaggc atttcgcttg cgatagggtg caattgcacg
      361 cttccagtat tacctacccg catccgctga cccatgaaaa gattattgtg acttcgccat
      421 tgccggaaga tttccggcaa tatgcatatt gatgcgacat gcatccggaa ttaaggatgc
      481 ggaaagaagg atttcatgaa aagaaagaca aaaattgtgt gcacgatcgg tccagccgtc
      541 gattcggagg aaatgatcgg aaagttgatt gaagccggca tgaatgtcgg aagaatgaat
      601 ttttcccacg gaacccacga agaacatctt cgtcgtctca acatggtgag atcagttgcc
      661 ggccgtatga acaaatatgt aggtgccatg gccgacacaa agggaccgga gatccggacg
      721 ggtgatttcc agaacggaca ggcttttttc ccgaaaggca gcacggttga aatcgtcaag
      781 gaagaaatcc tcggcgatga caaacatttc catatttccg ttcccgaact tttcgatgac
      841 atgaaagcgg gagattatct tcttatcgat gacggcaagg taagacttac cgtcactgaa
      901 aaaagagttg gctcgctagt atgcgttgtg aacaacgccg gcgtcatcaa aagcaaaaaa
      961 ggcgtgaatg tccccaatgt caaattgagc atgcctttca tttccgaaaa agacaaggac
     1021 gacatcagat ttgcttcaga aaacaaattc gacatgatag ccgtttcatt ctgccgccgg
     1081 aaacaggata ttctcgatgt tcgggcgttg cttgaacaaa acggatgcgg gaaaatggaa
     1141 gtgatcgcca aaatagagaa ccaggaaggt gtcgacaatc tttctgaaat cctcgaagtc
     1201 gtggacggta tcatggtggc gagaggagac ctcggggtcg aggtttcgac cgctcttgtg
     1261 ccgatatacc agaagaaaat catcaggatg gccaacgaag tgggcaaacc ggtcataacg
     1321 gcaacccaga tgttggaatc gatgatttcc tggcctcgcc cgacccgggc ggaggcctcc
     1381 gatgtcgcaa atgccattct tgacggttcc gacgcaatca tgctatcggg cgagacggca
     1441 gccggagaat atccaatcga agcggtccag acgatggata ccatcgccca ggctatagaa
     1501 gagatcatcc catatgacga acgtctccaa agatcgatca aaacatcgca atccacagtg
     1561 aacgacgcca tcggcatcgc tgtcagccag acggttttgt cgcttccttc ggccgaagtc
     1621 gtcgtcgctt tcacggaaac cggcggcacc gccaaacgga tgtgcaagtt ccggccggaa
     1681 gttccgatca tcgccatcac ggacaacgtg gagacatgtc gcaaactgtc ttattactgg
     1741 ggtgtcattc ccgtgcttaa aaaggatgtc acagattaca gccactatga cttatatgcg
     1801 atcgaagccg ccatcgggtg cggctacaaa ccgggaacga cgattgtcat gacatccggt
     1861 tggggccagg aacacggcca gacaaatacg atgcgtatca tcacgattcc gaatatttga
     1921 caaaccatta acaaaacaaa aaacagtctc ggatttccga aactgttttt attatgccat
     1981 actattccaa tttcattttg atgttcagtt tggtgaaaaa actttcaagt tttccggtta
     2041 gggacgactt gcggaatatc caatatgccg cttccgccaa tgctactcca gttataagat
     2101 cgatgatgta atgctgtttc aaagtctggg tggaaatgca aatcgttatt cccatcaccc
     2161 aaatgaagat tttggccggc agaggcattt tcttgtctat cctggctccg attatgcata
     2221 accagctcat cagacagtgc atagacggca tggcgttccg cggtccggcg gaggcatata
     2281 ttttcaatac cagacgctcg gtgaaattgg gatcctggat actgaaaaga ccggaactca
     2341 cccgccacat ttcaacatcg gtctgataaa agaaataaac caatccgcaa acggtgaaag
     2401 tcacgagaac caatgacacc agcagataca tattatgctt tgaacggtat cccacataga
     2461 agaaagtgaa tacccagaac ggataggaga gcacatacgg ataaatgctg taagaccaaa
     2521 agggaactgc ttggttgaaa ttgggaaata tgacactgag gtcgatcccg acgactccat
     2581 ttgcctctgc ataccactgg tttccccaat aagtgaaaaa ctgcattagg catacgagaa
     2641 caaagggcat caacacccgt gtaaagtttt tccagttcat aaaatacccc tttcccccct
     2701 ggaaatttca aaacaattat aacttacaaa aaagcaaaaa acaagcaaaa aaggcagttt
     2761 ggtcaaaagg ccgataattc ttgaaaagcc ttttgatatg tgataaaatc aagttaattt
     2821 gaaaatgggg gattttgcaa tgttcggact gagaaatgat ggcaaacgga tcaaaacgat
     2881 agatccggtt atgaagatgg ttccacatat catgaaaaca cgtaacgatg cgcaagtcat
     2941 ggcgctttac gagatagact gcaagggcct cgacgaatat attttcgcta aaagacatga
     3001 agaaaatatt cgtttcactt atatgcacat ccttatcgcc gctttcgtgc gtgtgatggc
     3061 tttacgtccg aaactcaatc ggttcatcgt caacggtcgg gttttcaaga gaaacgaaat
     3121 ccagatttct ttcgccgtca agaagacatt gcttgacacg gcggaagaga ctacgatcaa
     3181 gctgacgttt gacggaaccg aaagcattta cgatgttaaa aagaaaatcg acgatgcggt
     3241 tttggaaaac gccaaggaat ccgcagtcaa cggtaccgac aaaatggcaa aattgctgac
     3301 gatcgtccca aacggattga tcaaaatatt ggtcggcttt ctgatgtggc tcgataaaca
     3361 tggattgctt cccaaaaaag ttttggaagt atccccgttc catacctctc tattccttac
     3421 caacatgaaa tccatcaaga tgggttatgt atatcatcat tgctataatt tcggctcgac
     3481 ctcgattttc gtatcgatgg gcaaggaaaa atacgaacca gtcatattgg atgccgatga
     3541 tgccaagttc ggcgttgcca agatcatgaa agtcggagtg gtcgtagacg aacggatttg
     3601 cgatggttta tattacggaa actcattgcg ggagttcaag aaatatatgg aatatccgac
     3661 ggttctcgag gaaagaatcg atcacaagat cgaggaccag aaataatcgg gaaagatcgg
     3721 gaaagaggta ggcaatggat ttattcatga aaatactcgg aatttgcatc ggattgttct
     3781 ttcttttcat cggaatccgt tttgccttcc gcagcactaa cgttatcaac ggtatccaga
     3841 gatacaagta ccatgagacg caaacgccaa gaaaacagga actattgttt gccagaatca
     3901 tcggtgtatt gctcattttg gtcggactgt atttcaccat gatagccgtt ctatccctct
     3961 tccctgactc atgacggagt aaaaagattt tttagtggtt gtatattccg cttgcggatg
     4021 atacaatagt ttttggaaga cgtggcaatg ctgtgccaaa taatgtctgc ctggggatgt
     4081 gaagagatga atatcatcag actaaacgat atagaaaaga catttggtgg aacattgctt
     4141 tttaaacatg tatccttcga ggtgaacgac gacgataaag ttgccgtcat cgggaagaat
     4201 ggcgttggca agacaacgct gttcaaaatc atgttaggtg cgatttcccc agattctggg
     4261 gaaattttta tttctaggca agctcgcatc ggttatctta gccaagacgt tctagagcct
     4321 ggcgaaagaa cgcttttcgg agagatgttg ttattatatc aggaacttga aaaccttggt
     4381 cagaatctcg accaagccgc taaggctcta gcaacggatc attccgataa agcgatggaa
     4441 aggtacagcc gtttggaaac agaatttcag atcaaaggcg gataccagta caaaaccgat
     4501 attgaaacca tgctgacacg ctttggcttt tccagagaag atcacgaccg gtttgtttcc
     4561 gggttctccg ggggagagaa gaccaggatc gcttttgcga aactgctcct catgcatccg
     4621 gacattctct tacttgacga gccaaccaac catatggata ttgagatcat cgaatggctg
     4681 gaagattatc ttaagaaata ccaaggggct gtcgtcgtcg tcactcatga ccagtatttc
     4741 attaacaaag taactcatat tatctatgag ctggatcagg aaacgatgaa ccgctatacc
     4801 ggaacatatg aagaatacga agccgaaaaa atgaaacgct acgaattgct tatgaagcaa
     4861 tatgtccgtc agcaaaagga aattgctcac ttgcaaagct atgtagatcg gtttcgctac
     4921 aaagccaata aggcaagcca ggcgcaggat cggatcaaga agattgctaa aatcgatagg
     4981 atcgataagc caaccaaaag cgaaagggaa atatcctttg ctttcagaac gccacgtccg
     5041 accgatgcca tcattctgga agcaaaaaat ctgacgatcg gatataataa gcctctgatc
     5101 gaaaatattt cttttcagat gcgcggacgt gacaagatcg gcattatcgg gcaaaacggc
     5161 gctggtaaaa ccactttggt aaagacatta ctaggaacca tttctgccct caagggaaat
     5221 atagcgtttc ccaaacaact taaaatcggc tattttgacc agatggcgac tgaaaaccgg
     5281 atgaaaggaa aattgatcga tatcgtccat gacatttatc cacaaaagaa tttgctcgaa
     5341 gtgagaaaaa ttttagcgag attccagttc gtgggagaag atgtcttcaa ggaaaccgag
     5401 atgttgtccg gaggcgaaat ggtcagactt agactcttgt tattgctgtt ggaagctccg
     5461 gaatttttgg tgcttgacga accaacaaat catctcgata tctccaccaa ggacatcgtc
     5521 gaagacgtat ttgccgaata cgacggcccg attgtattca tatcgcatga ccgttacttc
     5581 attaacaaag tagccaccag gatcatcgaa ttcgatgaga cgaagtggca actttatgaa
     5641 ggaaattacg atttttataa agaatcaaaa gggaataaca ttccaactgc ggaaaaaaaa
     5701 gaaaaaaaac caaggaccgt tgatccggaa aaggaaatca ggaaattgga atcgtccttg
     5761 gaagaaaatt cggtccgcat ctcggtgttg caaaaaagct tatttcagcc ggaaatctat
     5821 atggacaaaa gcaaatatac tgagacagaa accgaaatca agcttttaga aactactact
     5881 ataaacatta tgaaacgaat cgaggaattg tcatcctgac agaaatcaga ttcggaaaaa
     5941 tagtaagaat cataccgctg tttgcacttg
//
LOCUS       DAOPJL010000047         5856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_14652_length_5856_cov_7.168419, whole genome shotgun sequence.
ACCESSION   DAOPJL010000047 DAOPJL010000000
VERSION     DAOPJL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14652_length_5856_cov_7.168419"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PLP02_05515"
     CDS             <1..954
                     /locus_tag="PLP02_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42933.1"
                     /translation="LKAIYGIITIGSVVSYVASITLISKSVGDFLSRYARMHLFNDYL
                     DNYFTYLNLDTKQVYGDVEYVEPKHLEITFDNVSFKYPNVDEYALKNLNFTISNYEKV
                     AIVGPNGAGKTTLVKLLCRLYDASEGEIKINNIPIQNYSKEALYRLYSIVFQDFNLFS
                     YSIEDNIAIGNEIDRDKLDKVIDEVGLSKRIKEMKDGTKTILFQRNKEAGIEVSGGEA
                     QKLAIARALYKESPFVILDEPTAALDPRSEAEIYDKFNLLVNNKTSIFISHRMSSTKF
                     CDRILVLDNGEIAETGNHESLVKQNGLYNQMWTAQAKYYQK"
     gene            1060..1707
                     /locus_tag="PLP02_05520"
     CDS             1060..1707
                     /locus_tag="PLP02_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF42934.1"
                     /translation="MSNIDRTIKYVQDLFDKSEYLKDKPKQKEYRLEHTYRVASIGKE
                     IAQKENLDIDALVVGCLLHDISYIYEMESEDDSLNHGRKSAQIAREFVMGLDMDAKLK
                     NELLYGIAIHVDDKADFEGERTVLAESIGEADNIDRFDRYRLYENLLYSNLSNMSLDE
                     QMGYASKRVERLKNLKKYVFRTKTSNRMWNEKLDYQIGYYEGLLEQLQKSDCEKL"
     gene            1779..2702
                     /locus_tag="PLP02_05525"
     CDS             1779..2702
                     /locus_tag="PLP02_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42935.1"
                     /translation="MSFEIQKVKRIDESLLKELYGRHYYNGFISNNWSMEMVESITQD
                     IIKHGVAFELREDGQPLGFISAYKLDKLFYGQKGYYTPELGWMYNNDYVVLNLLLKAL
                     LADMAEEGYTHHAIGMFYPDNEDQWFNYGYGIRKVDVSRKIGHEETEIALVNVKPEDI
                     DVFYPLYQKFEQYMMGSPIFLTGDDSKDACEKAIKDKKDKLFFLKKGETILGFTTLSE
                     TEAAGSPIFKDDKTMAIKGTYILEQYQGQGYGEKALCAIMNYSVEKGFEGLSTDFESY
                     NLRALRFWPRFFAIAAKTFVRYIGTPLKKAE"
     gene            2885..>3179
                     /locus_tag="PLP02_05530"
     CDS             2885..>3179
                     /locus_tag="PLP02_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF42936.1"
                     /translation="MNNIGDNIKNQRNKKNITQQALADELFVTRQCVSRWEQGKTLPD
                     IVTIEKLATVFSCSVNDLIDDNSVKAITIDQAIMNKRRIRIIWITLVSSISLIV"
     assembly_gap    3180..3279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3280..4017
                     /locus_tag="PLP02_05535"
     CDS             <3280..4017
                     /locus_tag="PLP02_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42937.1"
                     /translation="KVKIRYDENADFYDITVIDSLVGESLYGIYISGTGTDYVSVDMI
                     RASLSGVNYFMRRQDGEEAIFTTGNYYNFSFENDGYYHEYIYDIYISLNPLYDEHAYE
                     IGVITSNGLRSTETIDLSNRQSEYTVTGAFVLESSQYDYADNVDMTCNIHVNWVYPYA
                     SIEIYEYDKNNVLVSEKNIETLSELRDFEASDTALRCLIKVNTLEISGSGIRENSQVY
                     ELLLGESLDLFQSGDYGFVTNEQLTYD"
     gene            4121..4309
                     /locus_tag="PLP02_05540"
     CDS             4121..4309
                     /locus_tag="PLP02_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPF42938.1"
                     /translation="MKKNVGTLDRIIRVVLGAGFVVLGFLVSYWFMIGAVIMFLTAAT
                     GTCPLYIPFGINTNKKNK"
     gene            complement(4415..>5856)
                     /locus_tag="PLP02_05545"
     CDS             complement(4415..>5856)
                     /locus_tag="PLP02_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42939.1"
                     /translation="ENGYITTYTVGEVDTYGSLCTADYSLIATDSDNGFDTNFLIRLY
                     LNAGTYYITVSGEENYYEGDYRVCVEYSAESDIDFVSLSDAYLDIEETDRYEIVVSES
                     GYLVALTMSDMDMCGELYTADETELCSDDDGGYGFNFLIYYYLTPGTYYVDAYGYETD
                     VDGDYQFIAFIKTGSDSDHYYAVNTEHIASAYDSYPITITEDGYICAYTLGALDTYGY
                     LYTDDNTLIAEDDDSGIERNCQVASFVTAGTYYVVVTGYNATEAGDYRFVVDFEPANV
                     YPHLLCEDEYLDSGDNDFYLIEITEPGYLMAFTVGDVDTLGYLYDSWYDEILQNDDSG
                     GNYNFLLLYHVVPGSYYVCVSGYDAIESGDYRLIVDFVSDAEGGVFGYFFSAYGDTSV
                     SSGDRYELTLPEPGIITIYTISSLDTYGYLYSSENVLLLGNDNSSEGDNFLILYNAEA
                     GTYYIDVEGLDADKDGPYLLIVNYQLFNK"
BASE COUNT         1774 a         1179 c         1317 g         1486 t
ORIGIN      
        1 ctcaaagcaa tctacggtat catcacgatc ggaagcgtgg tgtcatatgt ggcgagtatc
       61 acgctgattt ccaaaagcgt cggagatttc cttagtagat acgcaagaat gcatctgttc
      121 aatgactacc ttgacaatta cttcacttat cttaaccttg atacgaagca ggtttacggt
      181 gatgtggaat atgtcgagcc aaagcatctg gaaatcactt tcgacaacgt atctttcaag
      241 tatcccaacg tggacgaata tgctttgaaa aacctgaatt tcacgatatc gaattatgaa
      301 aaggtagcga tcgtcggccc taacggggcc gggaagacaa cgctcgtcaa attactttgc
      361 cggttgtatg atgcttctga gggcgaaata aagataaaca atataccgat tcagaattat
      421 tccaaggaag ccctttacag gctgtatagc atcgtcttcc aggacttcaa tctgttttcc
      481 tacagcatag aggacaatat agccataggg aacgagatcg atcgggacaa actggataag
      541 gttatagacg aagtcggtct tagcaagcgg atcaaggaaa tgaaagacgg aaccaaaacg
      601 attcttttcc agcgaaacaa agaggccgga atcgaagtca gcggcggcga ggcacaaaaa
      661 ctggcgatcg ccagagcgtt atacaaagaa tcgccgtttg tcattctgga tgaacctacc
      721 gctgctttag atcccaggag cgaagcggag atttatgata aattcaatct gcttgtcaat
      781 aacaagacct cgatattcat atcgcaccgg atgagcagca ccaaattctg cgaccgcatt
      841 ctggtattgg acaacggtga aatcgccgag accgggaacc acgagtcgct cgtcaagcag
      901 aacggacttt ataaccaaat gtggaccgcc caggccaagt actaccaaaa atgacagaga
      961 taaagaaaca aaatagaaag ccgatttggc tttctatttt ttattcgcat ttgttcatcc
     1021 gtattcggta tgatataatc aacataggcg gtgatattga tgagcaacat cgacagaacc
     1081 ataaaatatg ttcaggatct gtttgacaaa agcgagtacc tgaaagacaa gccaaaacaa
     1141 aaggaatacc gtttggaaca tacctacagg gttgcctcta tcggtaagga aatcgcacaa
     1201 aaggaaaatc tggatattga tgctttggtg gtcggttgcc ttttgcacga catcagttat
     1261 atctatgaaa tggaatccga agacgatagt ttgaaccacg ggagaaaatc ggcccagatc
     1321 gccagggaat tcgtcatggg tctagatatg gacgcaaagc ttaaaaacga gcttctgtac
     1381 ggaatcgcga tccacgtgga cgacaaagcc gatttcgaag gcgaacgcac ggtattggcg
     1441 gaatctatcg gcgaagcgga caacatcgat cgttttgatc ggtatcggtt atatgagaac
     1501 ctgctttatt cgaatttgtc gaacatgtcg cttgacgagc agatgggata tgcctctaaa
     1561 cgggtggaac ggctcaaaaa cctgaaaaaa tacgtgttca ggacaaagac gtccaaccgg
     1621 atgtggaacg aaaaactcga ttatcagatc ggatactacg aaggtttgct ggaacaatta
     1681 caaaaaagcg attgcgaaaa gttataagcc ataaagcatt taccaattat ggtgatttta
     1741 taaatgggaa cacaatacca aaagaggggg atttctttat gtcatttgaa attcagaaag
     1801 taaaaaggat cgatgaaagc ttgcttaaag aattatacgg gaggcattat tacaacggtt
     1861 tcattagcaa caactggtct atggagatgg ttgaatcaat aacccaagac attatcaaac
     1921 acggagtggc tttcgaactg agagaggacg gacaaccctt aggcttcatt tcggcataca
     1981 agctcgataa attgttttac gggcagaaag gctattatac gccggaactg ggatggatgt
     2041 acaataacga ttacgtcgta ttgaatttgt tgttgaaggc attgcttgcc gacatggcgg
     2101 aagaaggtta cacgcaccac gccatcggca tgttttaccc cgataacgaa gatcaatggt
     2161 tcaattacgg atatggcatt agaaaagtcg atgtttcgcg gaagatcggt catgaagaaa
     2221 cggaaattgc gcttgttaat gtcaaaccgg aagatatcga cgtattttat ccgctttacc
     2281 aaaaattcga acaatatatg atgggatcgc caatttttct aaccggtgac gattctaaag
     2341 atgcatgcga aaaagcgatc aaggataaaa aagacaaatt attctttttg aaaaaagggg
     2401 aaacaattct gggattcaca acattaagtg aaaccgaagc ggcaggatcg ccgattttca
     2461 aggacgacaa aacaatggcg atcaaaggca cgtatattct ggaacaatac cagggtcagg
     2521 gatacggaga aaaggccttg tgcgccatca tgaattattc ggtggaaaaa gggttcgaag
     2581 gactttcgac cgactttgaa tcatataact taagagccct tcgtttctgg ccgcgttttt
     2641 tcgctatagc cgcaaaaaca tttgtccggt atatcggcac gcctctgaaa aaagcggaat
     2701 gaaatcgttt ctgacatcaa atgatccgtt caatgaattg agcggatttt ttatttggca
     2761 tatagatatg attccagaac aaaagccggg agtttgactt aaaagaccca gaaacaaact
     2821 gttgcggcga tattgcgtat catttattag tttttatgcg ataatactgt cgaggtgaca
     2881 tgcaatgaac aatatcggtg acaatataaa aaatcagaga aacaaaaaaa atatcaccca
     2941 gcaagccctg gccgacgaat tattcgtgac aagacaatgc gtttccaggt gggaacaggg
     3001 aaaaactctg cctgatatcg tcactatcga gaaattggca accgttttta gttgctctgt
     3061 caacgatctt atcgacgaca attcggtcaa ggcgatcacg atcgatcagg cgataatgaa
     3121 taaaaggcgg atcaggatca tctggataac tttggtgtca agcatctcgc ttatcgtatn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aagtcaagat aaggtatgac
     3301 gagaatgccg atttttatga tatcaccgtc atcgattcct tggtaggcga atcgctttat
     3361 ggcatctata taagcggaac cggaacagat tatgtttccg tggatatgat cagggcaagt
     3421 ctatctggtg tcaactactt catgcgccgt caggacggtg aagaagcgat cttcacgacc
     3481 ggcaattact ataatttcag tttcgaaaac gacgggtatt accatgaata tatctacgac
     3541 atatatatca gtcttaatcc tttgtatgac gaacatgctt acgaaatcgg cgtaattact
     3601 tcgaatggat tgagaagtac agaaacgatc gatctttcaa accggcaatc ggagtatact
     3661 gtgaccggcg cttttgttct tgagtcttca caatacgatt atgcggataa cgtagatatg
     3721 acgtgcaaca tccatgtcaa ctgggtttac ccctatgctt caatcgaaat atacgaatac
     3781 gacaagaata acgtattggt aagcgaaaaa aacatcgaga ccctatccga actgagggat
     3841 ttcgaagctt ctgatacggc attgcgttgc cttatcaagg taaacacatt agaaatatcc
     3901 ggtagcggga ttagagaaaa ttcacaggta tacgaactat tactgggaga aagcttggat
     3961 ctttttcagt ccggcgacta tggttttgtg acaaacgagc aactgactta cgattgatgt
     4021 atttgtataa acgattgtcc atggatctgc ttgtttctcg cgagcagatc ttttggtttt
     4081 atttggcaag cggtttataa tgtaaataga ggtgataatt atgaagaaaa acgtcggtac
     4141 gctcgacaga atcatcagag tggttctggg cgccggattc gtcgttctcg gatttctggt
     4201 cagttactgg ttcatgatcg gtgctgtcat catgttcctt accgcagcca ccggaacatg
     4261 tccgctttat atcccgttcg ggatcaacac caacaaaaag aacaaataaa aatgcattta
     4321 cctcccctta atgcagacga taaaaagaaa agagccatgg attccatgac tctttttttg
     4381 tgatgtatat gaaaattatg atgaaataat ccgcttattt gttaaaaagc tggtaattca
     4441 cgataagcag atacggaccg tccttatcgg catccaatcc ttcgacgtcg atataatacg
     4501 ttccggcttc ggcattgtac aggatcagga aattgtctcc ttcggatgag ttgtcatttc
     4561 ctaacaaaag gacattttcc gaggaataca gataaccgta tgtgtccagg gaactgatgg
     4621 tatagatggt aatgatgccc ggttccggca aagtgagttc gtatctgtct cccgagctga
     4681 ccgaagtatc accgtaagcg ctgaagaaat agccgaatac gccgccttcg gcatcggaaa
     4741 cgaaatcgac tatgaggcgg tagtccccgg attctattgc atcgtatccg gatacgcaga
     4801 cataataact acccggaacg acatggtata aaagtaggaa attgtaattg cctccggagt
     4861 cgtcattttg caagatttcg tcataccagg aatcataaag atatccgagg gtatcgacgt
     4921 cgccaacggt aaaagccata agatatcccg gttcggtgat ttcgataaga tagaaatcat
     4981 tgtctccgga atccagatat tcgtcttcgc ataacagatg ggggtagaca ttggccggtt
     5041 cgaaatcaac cacaaaccgg tagtctcccg cttcggttgc gttataaccg gtcacaacga
     5101 cataatatgt tcccgccgtc acaaaactgg cgacttggca atttctttca attccggaat
     5161 catcgtcttc cgcgatcaaa gtattgtcgt cggtataaag ataaccgtat gtgtccaaag
     5221 cgccgagggt ataggcacaa atatatccgt cctcggttat cgtgatcgga tacgagtcgt
     5281 aagcgctggc gatatgttcg gtgttaacag cgtagtagtg gtcagaatcg cttcctgttt
     5341 tgataaacgc gatgaactga taatcgccat ccacatcggt ttcgtatccg taggcgtcga
     5401 catagtaggt tccgggcgtc agatagtagt aaatcaggaa attgaatcca tatccgccat
     5461 cgtcatcgga acaaagttcg gtttcatccg cggtgtatag ttccccgcac atgtccatgt
     5521 cgctcatggt cagggcaacc agatatccgg attccgacac cactatttca tagcggtcgg
     5581 tttcctctat atccaggtaa gcgtcggaaa gggaaacaaa atcgatatcg ctttccgccg
     5641 aatattcgac acagacacga tagtctcctt cataatagtt ttcctcaccg gaaacagtga
     5701 tgtaatacgt tccggcattc aaatacaatc taatcagaaa attagtgtcg aatccgttgt
     5761 cgctgtcagt tgcgataagg gaataatcgg ctgtacaaag gcttccgtat gtatcgacct
     5821 caccgacggt ataggtcgta atatagccat tttccg
//
LOCUS       DAOPJL010000048         5820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_14803_length_5820_cov_12.773981, whole genome shotgun
            sequence.
ACCESSION   DAOPJL010000048 DAOPJL010000000
VERSION     DAOPJL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14803_length_5820_cov_12.773981"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLP02_05550"
     CDS             complement(<1..662)
                     /locus_tag="PLP02_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPF42940.1"
                     /translation="MAFENLTSRFQMAFRRLVGKARLNENDIEEMLREIRLSLLEADV
                     NYKVVKEFTDEIKTLAFGEKILKGLNPDQQVVKIVNDELVKLMGSETSDINFRTGEPT
                     VIMMVGLQGTGKTTTAGKLANLLRKTKERKPLLVAADIYRPAAIEQLQTVGKQLDIPV
                     FSLGDKKDPVEIASEAMKEAKASGYDLVIIDTAGRLHVNETLMDELEAIKKKTKPDEI
                     LLT"
     gene            complement(667..990)
                     /locus_tag="PLP02_05555"
     CDS             complement(667..990)
                     /locus_tag="PLP02_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPF42941.1"
                     /translation="MEQIDEKIRMNKLYDLYGCLLTDKQRSYYTYYYFDDYSLSEIAK
                     IMDVSRNAVHEQIKNVTEILEYNENKLSLLAKKEKRLAILKQINPGLKKEELLDLFRE
                     MEKVE"
     gene            complement(1000..1977)
                     /gene="ftsY"
                     /locus_tag="PLP02_05560"
     CDS             complement(1000..1977)
                     /gene="ftsY"
                     /locus_tag="PLP02_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPF42942.1"
                     /translation="MGVFQNIFGNKQNRIKKEKYRLGMAKTRTGSLSNIRQLIESSPE
                     IGDALFDKLEEIFITADIGVDTAIGFIASLKQDVAKKKIADPKELEAMIVDKMFELYL
                     KGELVDTNIRYNPTGPTILLFVGVNGTGKTTSIGKLAYKIKAEGKTVMMAAADTFRAG
                     AIDQLKIWGDRVSCPVVVKPAGSDPSSVIFDAIREAKKNNIDVLLIDTAGRLQNKVNL
                     MKELDKMNRTIKRELPGAVYETYLVVDATTGQNGLNQARVFSEVTDITGIILTKLDGT
                     AKGGIVLAIRNELGIPISFVGLGEQITDLDYFDIENYIYGLFADFFEQE"
     gene            complement(2077..>3066)
                     /locus_tag="PLP02_05565"
     CDS             complement(2077..>3066)
                     /locus_tag="PLP02_05565"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HPF42943.1"
                     /translation="RLYRMIYSRAMASLMKSSVYEQTTVELANNNSIFQASGSKQLFD
                     GYLKIYGRYDGGEEKSKLPPLEKGTNLEAESIEKIRLETQPPARYTEARLIKEMEDLG
                     IGRPSTYAQTIQIIKQRRYVTLKEKKFIPTEQGRITIEQLDQYFKTLVTANFSKQMEE
                     TLDAIAEGEKYPVETLDKFYHYFQPLVDKAFKDMKKIKPKETGELCPKCGSKMVYQHG
                     KYGDFEACSNYPKCKYIKPKPESAVTRPKPFDTGIVCPSCHKGTLVERTAGKGKNKGK
                     KFYGCSNYPKCKYIAPYTSINAPCPSCGQPLVKDKQGKTICLDTEKCGYETDK"
     assembly_gap    3067..3166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3167..3491)
                     /locus_tag="PLP02_05570"
     CDS             complement(<3167..3491)
                     /locus_tag="PLP02_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPF42944.1"
                     /translation="MSEKLVIVESPAKSKTIGQYLGPDYIVKSSKGHIRDLAISGPGG
                     LGIDIEHDFKPEYKVLPEKKETVRELNDAMQKCDELLLATDPDREGEAISWHLKETLD
                     IKDKPV"
     gene            complement(3649..4506)
                     /gene="prmC"
                     /locus_tag="PLP02_05575"
     CDS             complement(3649..4506)
                     /gene="prmC"
                     /locus_tag="PLP02_05575"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPF42945.1"
                     /translation="MRSEPTYEGILKKAKKQARQIGLEESAVELLMLEFSGLSQTGLY
                     LHYRDAMPESDQIRFFKALDEYLVKGKPVQYVIGHVSFYGYDFIVNDSVLIPRFETEE
                     LVENVLSLTDEIFCGEQNIRVLDIGTGSGCIAIALKKEEPKYIVHATDISKEALEVAK
                     ENAHRMKAEIDFHNGDIFKGIPQIKFNVIVSNPPYIPENENVPRIIADNEPSVALYGG
                     DDGLRFHREILKEAKDYLSERFLIAFEHGYDKAILLRNIAKEYFPDCETYTKKDMQGR
                     DRMTFVVKR"
     gene            complement(4503..5567)
                     /gene="prfA"
                     /locus_tag="PLP02_05580"
     CDS             complement(4503..5567)
                     /gene="prfA"
                     /locus_tag="PLP02_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPF42946.1"
                     /translation="MLTERLDQIESKYNEISKQLMDPAVSTDIKKATELAKELSSLEK
                     FVVKYREYLDLQKQIRAVKSMQKDQDPEIREMAELELEELQQKILDTESELEIMLVPK
                     DPNDEKNVIVEIRGAAGGDEGNIFGGDLFRMYVKYAEAKGWKVEVLYAEPAEAGGYSQ
                     IEFMIKGDGVYSLMKYESGVHRVQRVPQTESQGRIHTSTATVLVVPEAEEMDFDVDME
                     DVRVDTFCSSGPGGQSVNTTKSAVRLTHIPSGLVVQCQDGKSQHENKASALKILRSRL
                     YDKMLQEKLDKEGKERKSKIGTGDRSEKIRTYNYPQNRVTDHRIGFTIQQLDRVIEGK
                     LDPIIEALVAEEQKRNMEAK"
     gene            complement(5614..>5820)
                     /locus_tag="PLP02_05585"
     CDS             complement(5614..>5820)
                     /locus_tag="PLP02_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPF42947.1"
                     /translation="IILLMYREDYYDKETTKKNHVEINIAKNRSGVIGDFELLFNPNV
                     STFSNLFVSAPEGGYEDRQPGDEF"
BASE COUNT         1199 a         1385 c         1306 g         1830 t
ORIGIN      
        1 gtcaaaagaa tttcgtccgg tttggttttt ttcttgatgg cttcaagttc gtccatcaga
       61 gtttcgttaa catgcaaacg gccggcggta tcgatgatca cgagatcgta tccggatgcc
      121 ttggcttcct tcatggcttc ggacgcgatt tctaccggat cctttttgtc tcctaacgaa
      181 aagaccggaa tatccagttg ttttcccaca gtttgcaatt gttcaatggc agccggacga
      241 tagatatcgg cggcgaccaa caatggtttt cgttctttgg tttttctgag aagattcgcc
      301 agtttgccgg cggtggtagt ttttccggtt ccttgaagac ctaccatcat gatgacagtc
      361 ggttcgccag tacggaaatt gatatcggat gtttccgaac ccatcagttt cacaagttcg
      421 tcgttgacta tcttcacgac ctgttgatcg gggttgagac ccttgagtat tttctccccg
      481 aatgctaaag tcttgatttc gtcggtgaat tccttgacga ccttgtaatt gacatcggct
      541 tccaacaaag aaagacggat ttcccggagc atctcctcga tgtcgttttc attgagtctg
      601 gcttttccga caagacgccg gaaagccatc tggaaacggc ttgtcagatt ttcaaatgcc
      661 atcttttcac tcaacctttt ccatttcccg gaaaagatcg agcagttctt ctttctttaa
      721 tccgggattt atctgtttaa gaatcgccaa tcttttttct tttttggcga gcagcgacag
      781 tttattttcg ttgtattcga gaatctcggt gacgtttttg atttgttcgt gcaccgcatt
      841 acggctcaca tccatgattt tggcgatttc ggagagcgaa taatcgtcaa aataataata
      901 agtgtaataa gaccgttgct tgtcggtcaa aaggcatccg tatagatcgt ataatttgtt
      961 catgcggatt ttttcatcaa tctgttccat ggttattttt cactcctgtt cgaaaaaatc
     1021 ggcgaataag ccgtatatgt aattttcaat atcgaaataa tccagatcgg tgatttgttc
     1081 gccgagtccc acaaaactga tcggaattcc gagttcgttt ctgatggcca ggacgattcc
     1141 gcctttggcg gttccgtcta gtttggtcag aatgattccg gttatgtccg tcacttcgct
     1201 gaacacccgg gcttggttga gaccgttctg tccggtggtg gcgtccacca ccagatatgt
     1261 ctcgtaaacc gctccgggca attcccgttt gatggtccga ttcatcttgt cgagttcctt
     1321 catcaagttg accttgtttt gcaagcgccc ggccgtgtca atcaacagca cgtcgatatt
     1381 gttttttttg gcttcgcgga tggcatcgaa aatcacgctt gaaggatcgc ttccggccgg
     1441 tttgacaact accggacacg atactctgtc accccagatt ttcaactggt cgatggctcc
     1501 ggctctgaaa gtgtcagctg cggccatcat cacggtttta ccttccgcct tgattttata
     1561 cgccagtttg ccgatcgatg ttgtttttcc ggttccgttg accccgacaa acaacaatat
     1621 cgttggcccc gtcgggttgt agcggatatt cgtgtcaacc agctcgcctt tcaaatacaa
     1681 ttcgaacatc ttgtcgacga tcatggcttc caattccttc ggatccgcga tctttttttt
     1741 cgccacatcc tgtttcaggg aagcaatgaa tccgatggcg gtgtcgacgc cgatgtcagc
     1801 cgtgatgaat atttcttcca gtttgtcgaa aagtgcatcc ccgatttccg gcgaactttc
     1861 gatcaactgt cttatgtttg acagagagcc ggttctggtt tttgccatgc cgaggcggta
     1921 tttttccttt tttatccggt tttgtttatt gccgaagata ttttggaaga cgcccattca
     1981 tacctcaccc cgttcatcaa aaacattata tcatactttc gccggcttgg gatatcataa
     2041 aagacatccg cctttcttgg gcgaatgtct tatggcttat ttgtctgttt cgtatccgca
     2101 tttttccgta tcgagacaaa ttgttttccc ttgcttgtct ttgacgagcg gttgcccgca
     2161 ggaaggacaa ggggcgttta tggaagtgta cggagcaata tatttgcatt tcgggtaatt
     2221 gctacaaccg tagaattttt tccctttgtt tttgcctttt ccggctgttc tttcgaccaa
     2281 tgtccctttg tggcaggaag gacagactat tccagtgtca aacggtttcg gacgtgtgac
     2341 ggctgattcc ggtttcggtt tgatgtattt gcatttcgga taattgctgc aagcttcgaa
     2401 gtctccatat tttccgtgct ggtaaaccat cttcgatccg catttcggac acaattcgcc
     2461 ggtttccttc ggtttgatct ttttcatatc cttgaaagct ttgtccacca gcggctgaaa
     2521 gtaatggtag aatttgtcaa gggtttccac cggatacttt tcgccttcgg ctatcgcgtc
     2581 tagagtctct tccatctgtt tggaaaaatt ggcggtcacg agcgtcttga aatactggtc
     2641 cagttgttcg attgttattc ttccctgttc ggtcggaatg aatttcttct cttttagtgt
     2701 tacatatcgc cgctgtttga tgatttggat cgtctgggca taggtcgacg gccggccgat
     2761 gccgagatct tccatctctt tgatgagtct ggcttcggta taacgggccg gcggctgagt
     2821 ttccaaccgg atcttctcga tcgattcggc ttccaaattg gtaccttttt ccaacggcgg
     2881 cagtttggat ttttcttctc ccccgtcgta tctgccgtat attttgagat atccgtcgaa
     2941 caattgtttg gacccggaag cctggaatat tgaattgttg tttgccagtt caaccgttgt
     3001 ctgttcgtaa acggacgatt tcatcaagga cgccatggca cgggaataaa tcatcctgta
     3061 tagtctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcac cggcttatcc
     3181 ttgatgtcga gggtttcttt caaatgccag ctgatggctt ccccttctcg gtccgggtcg
     3241 gttgccagca acaactcgtc gcatttttgc attgcgtcgt taagctcgcg tacagtttcc
     3301 tttttctccg gcaacacttt gtattccggt ttgaaatcat gttcgatgtc gattcccaaa
     3361 ccgccgggtc cggaaattgc caaatccctg atatggcctt tggaagattt gacgatgtag
     3421 tctggtccca ggtattggcc gatcgttttc gatttggccg gcgattcgac gataactagt
     3481 ttttcggaca ttgatatctc ccctttcctt gttaataata tatcgagtag ccctggtctt
     3541 gtcaatgatt ttcgcttgaa aaaaacggcc ttgtaacagc cttaaatagc taataattta
     3601 taaattatta aaaggaaaaa gaaattcccg ggattccggg aatctgattt atcttttgac
     3661 aacgaatgtc atcctgtctc ttccctgcat gtccttcttg gtgtaagttt cacagtcggg
     3721 aaaatattct ttggcgatat tccttaataa gattgccttg tcgtacccgt gctcgaaagc
     3781 gatcaggaag cgttccgaca gatagtcttt cgcctctttc aggatctcac ggtgaaatct
     3841 gagaccgtcg tcgccgccgt agagggcgac cgacggttcg ttgtcggcga tgatcctcgg
     3901 cacattttcg ttttcgggaa tgtaaggcgg gttcgagacg atgacattga atttgatctg
     3961 cggaatgccc ttgaaaatat ctccgttatg aaaatcgatc tcggctttca tccggtgggc
     4021 gttttccttt gccacttcga gcgcctcttt gctgatatcg gtcgcgtgaa cgatgtattt
     4081 cggctcttct ttctttaaag cgatggcgat gcatcccgag ccggtgccga tatcaagcac
     4141 cctgatattt tgctcaccac aaaatatttc gtcagtaagt gacaatacgt tttccacaag
     4201 ttcctcggtt tcgaatcttg gaatgaggac cgaatcgttc actatgaaat cataaccgta
     4261 aaatgaaacg tgtccgatca cgtactggac cggtttgccc ttcaccagat attcgtccag
     4321 ggctttgaag aatctgatct ggtccgattc cggcatggcg tcgcgataat gtaaataaag
     4381 tcccgtttgg gacaatcccg aaaattccag catcagcagt tcaacggcag attcctccaa
     4441 accgatctga cgagcctgtt ttttcgcttt cttcaagatt ccctcgtaag tgggttcgct
     4501 tctcatttgg cttccatatt ccttttttgt tcttcggcta caagggcttc gataatcgga
     4561 tcgagttttc cttcgatgac ccggtcgagt tgttggatgg tgaaaccgat ccgatggtcg
     4621 gtcacccggt tctggggata attgtaagtt cggatttttt ccgagcggtc gccggttccg
     4681 atcttgcttt tgcgctcctt gccctctttg tccaattttt cctgaagcat tttgtcataa
     4741 agacgggagc ggagaatttt caaagccgac gctttgtttt cgtgctggct cttgccatcc
     4801 tggcattgga ccaccaatcc agacggaata tgggtcagcc tgacggctga tttggtcgtg
     4861 ttgaccgact gccctcccgg cccggaagaa caaaaggtgt cgactcgcac atcctccatg
     4921 tcaacatcga aatccatttc ctccgcttcc ggaaccacca agacagtagc ggtcgatgta
     4981 tggattctcc cttgtgattc ggtttgggga accctttgga cgcggtggac acccgactcg
     5041 tatttcatca gcgaataaac tccatcgcct ttgatcatga attcgatctg gctgtagcct
     5101 ccggcttccg ccggttcggc gtataagact tccactttcc agcctttggc ttcggcatat
     5161 ttgacataca tgcggaaaag atcgccgccg aaaatatttc cttcgtcgcc tcctgccgca
     5221 ccgcggattt ccacgatgac gtttttctcg tcgttcgggt ccttcggtac cagcatgatc
     5281 tccaactccg attcggtgtc gaggattttc tgctggagtt cttccagttc caattcggcc
     5341 atctcccgga tttccggatc ctgatctttc tgcatggact tcacggcacg aatttgtttt
     5401 tgaagatcca ggtattcccg atacttcaca acaaattttt ccagcgaact gagttctttt
     5461 gccaattcgg tggccttttt gatgtcggtc gacacagcgg gatccatgag ttgtttgctt
     5521 atttcattgt atttggactc gatctggtcc agacgttccg taagcattgg taataccttc
     5581 cttttgatgt tttcggttaa aaaaaccggg tgatcagaat tcgtcgcccg gctgtctgtc
     5641 ttcgtatccg ccttcaggcg cgctcacaaa gagattgctg aaagtcgaaa cgttgggatt
     5701 gaacaacaat tcaaaatcgc cgatgacgcc ggaacggttc ttggcgatgt tgatttccac
     5761 gtgattcttc ttggttgttt ctttgtcgta atagtcttcc ctgtacataa gcaaaatgat
//
LOCUS       DAOPJL010000049         5438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_16231_length_5438_cov_7.986810, whole genome shotgun sequence.
ACCESSION   DAOPJL010000049 DAOPJL010000000
VERSION     DAOPJL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16231_length_5438_cov_7.986810"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..613)
                     /locus_tag="PLP02_05590"
     CDS             complement(62..613)
                     /locus_tag="PLP02_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42948.1"
                     /translation="MNKGLISGLVLLILGLVCGLLLATVNYFTDPIIQAAEEAAKYEA
                     LNTMLADFDTRSTDDFDITEVSLSGHVDTAYWLYDNTTNALDYIVYSVSAAGYQSDIV
                     MLIAIDPYMIVQGYTIVSEGETAGVVDYIYTIDYKMVGRSITNVVSLDDISSVTTAHF
                     SLAAVLNDFQYVSEQAVIDLGGE"
     gene            complement(615..1232)
                     /locus_tag="PLP02_05595"
     CDS             complement(615..1232)
                     /locus_tag="PLP02_05595"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPF42949.1"
                     /translation="MKKFLSKSGAWIVMTILLVLIFVFGTLYSLYMTNQISVHEYEAY
                     LVYYQDASKAEGFDTTAVEASYQIPGTTGDENMFTPTLDRSIKVYSGDEVIGVIYVIT
                     SHGNADGLEILVAISTETDSVENIAVLSQNETDDSNRYYGSLTDANFFDQFAGLSFDT
                     PDITLDTVAGATKSCEGFEIAIEYARLQYAADFGFVIPEGNGGAS"
     gene            complement(1234..2415)
                     /locus_tag="PLP02_05600"
     CDS             complement(1234..2415)
                     /locus_tag="PLP02_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPF42950.1"
                     /translation="MARFAGGKAPFLRSVDQGGQDTKKIMRDFLIGLIPVILFAWYKN
                     GIQVYINGDCTIWEMLYPLAFILLGGIISMLMEGLVFVITDKETRSLKAIMQKINLSF
                     AVVPGLLIALILPMYTPIWVLVFGCFMGTVVGKMLFGGFGHNIFNPALVGYLAIGFTF
                     SGLIGNSGGYLNPSEVLVDSYAGATPLGTLAMFKTISWDTLVAPYGTLWNFFLGTIPG
                     SLGETSALAILVSFIWLSARKVIKWSTPVIYVGTVFILSWIIGIASGDSGLWFPSYSI
                     LSGGLMFGAVFMATEPVTTPKNPLGKIIYALLLGVLTVLFRMIGNYPEGVATSLIVMN
                     IFTIPVDQVTAEIRANGITKKTWVKAAVMLLIILGIGFYAVTKSVNTYTIFVGFFGGV
                     M"
     gene            complement(2416..3720)
                     /locus_tag="PLP02_05605"
     CDS             complement(2416..3720)
                     /locus_tag="PLP02_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HPF42951.1"
                     /translation="MILRKAKGKHIEGKKQMSRCQPLQEYFNPPFVYLPLVHQNAVLK
                     RVVEVGDDIKVGQTVAVREGFGAMPFHASVSGKVSAVKKVWHASGKMVEAIEIENDNL
                     NRMDDSIKPEKNVEGLTKEQLIKKMQDAGLCGMGGASFPTYVKYMAKCPIDTIIINDV
                     ECEPYLTCDCMLSLNYPEKVLKGLKYFMKAADATQGVIAIKNYNRNVIEMFAILLNDY
                     PNVRIQEMNDIYPAGWEKYIVEQVTGKTYEKLPSEAGVIVNNSATAAAFADMVENNIP
                     LISRIITITGEGIQTPQNFHVPIGTKVAELIAKTGGYIEGLDTTKSWYIAGGPMTGRA
                     ILIDDLIVDYPLGSVIVMPMPEEKDHPECLGCGRCADVCPVFLTPTSIKSAYERKDIA
                     AIGKLNAMKCMGCGLCSYVCPSHVEITDFVAKAKDMYRKGAK"
     gene            complement(3809..4540)
                     /locus_tag="PLP02_05610"
     CDS             complement(3809..4540)
                     /locus_tag="PLP02_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014653058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPF42952.1"
                     /translation="MRINVYLSSRGLCSRREADRLIEQGEVTVNGKPARLAMDIADTD
                     DVIFKGKPVAKTTKDNVYILLNKPRGIISTTEAEKKDNIISYIHYPRRIFPVGRLDKD
                     SSGLIILTTDGQIVNKILRSENRHEKEYVVRTWRPFDKDFIVEMASGVNIYNPVRHRI
                     ETTKPCKIKKLENDLFSIILTQGLNRQIRRMVKALGNEVIELERVRIMNLEKGDLSEG
                     SWRYIEGRELETLMSLVGENSQNTK"
     gene            complement(4530..5048)
                     /locus_tag="PLP02_05615"
     CDS             complement(4530..5048)
                     /locus_tag="PLP02_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF42953.1"
                     /translation="MKRIIFKIYRQPDINLSGSTLHRFAFRGIIWFGDKLLMIQSKKY
                     GEMKFPGGGKEKGEKTLKTLRREVLEETGYVIKPHPRYFGHTIEYARDYKGEYDVFKQ
                     ESGYYFCEVYPENKGTAYSSGETAYGYHPILVTLDEAIKNNENVQSNDIIPWKERDTK
                     MLYTLKEMQNAD"
     gene            complement(5050..>5438)
                     /locus_tag="PLP02_05620"
     CDS             complement(5050..>5438)
                     /locus_tag="PLP02_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPF42954.1"
                     /translation="GSKDFIKKARIKRKIMGGGMRQVGILAACGKISLDRMTKRLNED
                     HENAKYMAELLNQIKGVAIDETQRDINMVFFDIDDKRKYKLEEYLYNHGVKILPYEDG
                     FRFVTHNDVSMADVKMAINLVKAYFN"
BASE COUNT         1352 a         1341 c         1235 g         1510 t
ORIGIN      
        1 gatacgaaga tcgggttttc cttgacgaat cccttgaaga agttctgttt ctggttcatc
       61 atcattcacc tcccagatcg attacagctt gttcggacac atattggaaa tcattcaata
      121 cggccgcaag cgagaaatgg gcggtggtga cagacgatat gtcatcgagc gacaccacat
      181 tggtgatcga tctgccaacc atcttataat caatagtata gatgtaatcg acgacaccgg
      241 cggtttcgcc ttcgctcacg atcgtgtaac cttgaacgat catgtatgga tcaatcgcta
      301 tcaacatcac gatatccgac tgataaccgg cggccgagac gctgtatacg atataatcta
      361 aagcgtttgt cgtattgtca taaagccagt aagctgtatc cacatgaccg gataaagaga
      421 cttcggtgat gtcaaaatca tccgtgctcc tagtgtcgaa atccgccaac atggtattta
      481 atgcttcata cttggcggct tcttccgccg cctggatgat cgggtccgtg aaataattga
      541 cggttgcgag caataacccg caaacgagtc cgaggattaa aagcaccagc ccggaaatca
      601 aacctttatt catatcacga agcacctccg ttcccttccg gaatcacaaa gccgaaatcg
      661 gcggcgtatt gcaatctggc atattcgatc gcgatttcga atccttcgca ggatttcgtc
      721 gctcccgcaa cagtgtccaa ggtgatatcc ggtgtatcga acgacaaacc ggcgaattga
      781 tcgaagaagt tcgcatccgt cagactgccg taatagcggt tggagtcgtc cgtttcattc
      841 tgactcagaa cagcgatatt ttcgaccgaa tcggtttcgg tcgaaatagc gacgagaatt
      901 tcgaggccat ccgcgtttcc atgtgatgta atgacataga tcactccgat gacttcgtcg
      961 cccgaataga ctttgatgct tctatcaagt gtgggagtga acatgttttc atccccggtg
     1021 gttccgggaa tctggtaaga tgcttcaacg gccgtcgtgt caaatccttc ggctttggag
     1081 gcatcctggt aatagacaag atatgcttcg tattcatgta cggatatctg gttggtcatg
     1141 taaagactgt aaagcgttcc gaaaacgaat atcagaacga gcaggattgt catgacgatc
     1201 caggctccgg acttagacaa gaatttcttc atcttacatc acccctccga aaaaacctac
     1261 gaagatagtg tatgtattga ccgatttcgt cacggcatag aatccgatac caaggatgat
     1321 caaaagcatc acggctgctt tcacccaagt cttcttggtg atgccgttcg ctctgatttc
     1381 cgccgtcacc tgatcaactg ggatcgtgaa gatattcatg acgataagcg aagtcgcaac
     1441 tccttccggg taatttccga tcatccggaa gaggacagtc aaaacgccca gaagcaaggc
     1501 atagatgatt ttacccaaag gattcttcgg agttgtgacc ggttcggtcg ccatgaaaac
     1561 ggcaccgaac atcaaaccgc cggacagaat gctataagac gggaaccaaa gtccggaatc
     1621 gccgctggca ataccgatta tccaggaaag gatgaacacg gtgccgacat aaatgaccgg
     1681 ggtcgaccat ttgataacct tgcgggccga caaccagatg aaactgacaa ggattgccaa
     1741 ggcgctggtt tcgccgagcg atccgggaat cgtacccagg aagaaattcc aaagtgttcc
     1801 gtatggtgcg acaagcgtgt cccaggaaat cgtcttgaac atggcaagtg ttcccagggg
     1861 cgtcgctccg gcataggaat cgaccaacac ttccgaagga ttgagataac cgccggaatt
     1921 gccaataagc ccggagaaag taaaaccgat cgccagataa ccgacgagcg ccggattgaa
     1981 gatattatgt ccgaatccgc cgaaaagcat cttcccgaca actgtgccca tgaagcatcc
     2041 gaagacaagc acccagatgg gcgtatacat cggaagaatc agggcaatca acaagccggg
     2101 aaccaccgca aaagacagat ttattttctg catgatcgct tttagcgatc ttgtctcttt
     2161 gtcggtgatg acgaacacga ggccttccat cagcatggag atgatcccgc ctaacaaaat
     2221 gaaagcgaga ggataaagca tttcccaaat cgtgcaatca ccgttgatat atacctgtat
     2281 accgtttttg taccaagcga agagaatgac agggatcaga ccaatcagaa aatcacgcat
     2341 gattttctta gtgtcttgtc ctccctggtc aacgcttctc agaaaggggg ctttgccgcc
     2401 tgcaaatctg gccatttact tcgctccttt ccgatacatg tctttggctt tcgccacaaa
     2461 gtcggttatt tccacatgcg atgggcagac ataggaacaa agtccgcatc ccatgcattt
     2521 catggcattg agtttgccga tcgccgcgat gtcttttctc tcatatgcgc tcttgatcga
     2581 agtcggagtc aggaacaccg gacaaacatc ggcgcagcgt ccgcagccaa gacattccgg
     2641 atgatccttt tcttccggca tcggcatgac gatgacggat cccaaaggat agtcgacgat
     2701 gagatcgtcg atcaatatcg cccggccggt catcggaccg ccggctatat accacgattt
     2761 cgttgtatcg agtccttcga tatacccgcc ggtcttggcg atgagttctg ccactttggt
     2821 tccgatcgga acatggaagt tctgcggtgt ctgaatgcct tcacccgtga tcgtgatgat
     2881 ccgggaaatc aacggaatgt tattttccac catatcggcg aaagcggcgg ccgtcgcgga
     2941 attgttcacg atgacgccgg cttccgatgg tagtttttca tatgtttttc cggtcacttg
     3001 ttcgacaatg tatttttccc agccggccgg ataaatatca ttcatttcct gaattctcac
     3061 attcggataa tcattcaaga gtatagcgaa catctcgatt acgttccgat tgtaattttt
     3121 gatggcgatg acgccttgcg tggcatcagc cgccttcatg aaatatttga gccccttaag
     3181 cactttctcc ggatagttga gagaaagcat gcagtcgcag gtaaggtacg gttcacactc
     3241 gacatcattg atgataatgg tgtcgatcgg acacttagcc atgtacttga cataggtcgg
     3301 aaaactggcc ccgcccatgc cgcaaagtcc ggcatcctgc attttcttga tcagttgttc
     3361 tttcgtgaga ccttcgacgt ttttctccgg tttgattgaa tcatccatcc ggttcaaatt
     3421 gtcattttca atctcgatcg cttccaccat tttcccggaa gcgtgccaga ctttcttgac
     3481 ggcgctgact tttccgctta ctgaagcatg gaaaggcatg gctccgaaac cttcgcgcac
     3541 agccactgtt tgtccgactt tgatgtcatc gcccacttcc accacgcgtt tcaaaacggc
     3601 gttctgatgg acaagtggga gatacacaaa aggcggattg aagtattcct gcaatggttg
     3661 gcatctggac atttgtttct tgccttcgat gtgtttgcct ttcgctttac gcaaaatcat
     3721 tggcgatatc ctccttcaag acatggtaag catctttcac agccacaaag tattataaca
     3781 ctattcatat tatttattaa cactaaattt atttggtgtt ttgactgttt tcaccaacaa
     3841 gcgacatcaa agtttccagt tccctgcctt cgatataacg ccatgatccc tccgaaagat
     3901 cgcctttttc caggttcata atccgaactc tttcgagttc gatgacttcg tttcccaagg
     3961 cttttaccat tcttctgatc tgccggttca atccttgggt aagaatgatt gaaaacagat
     4021 cattttccag tttcttgatc ttgcacggtt tggttgtctc gatccggtga cgcaccggat
     4081 tgtaaatatt gactccggat gccatttcga caatgaaatc tttgtcgaac gggcgccagg
     4141 ttcttacaac atattccttc tcgtgacggt tctcgcttct taagatcttg ttcacaatct
     4201 gtccgtccgt cgtcagaatg atgagaccgg aagaatcctt gtcgagtctt ccgacgggaa
     4261 agattcggcg cggataatgg atataagata tgatattgtc ctttttttca gcttccgtcg
     4321 tcgaaattat ccctctgggt ttattcaaaa ggatatagac attatctttg gtggttttgg
     4381 caaccggctt gcctttgaaa atgacatcgt ctgtatcggc gatgtccatg gcgagtcggg
     4441 ccggttttcc gttcaccgtc acttcgccct gttcgatgag acggtcggct tcccgtcttg
     4501 agcagagccc gcgggaactt aagtagacat taatccgcat tttgcatttc ctttaaagtg
     4561 tataacattt tggtgtccct ttccttccag ggaatgatat cgttcgattg aacgttttcg
     4621 ttgttcttga ttgcttcgtc tagcgtcacc aaaatcggat gatagccata tgcggtttcc
     4681 ccggaagaat atgcagtccc cttgttttcc ggatacacct cgcaaaaata atatccggat
     4741 tcctgtttga agacatcgta ttcgcctttg taatctctgg cgtattcgat tgtatgtccg
     4801 aaatagcgcg gatgcggctt tatcacatat ccggtttctt ccagaacttc ccttcttagt
     4861 gtctttaagg ttttctcccc tttttccttg ccgccaccgg ggaatttcat ttccccgtat
     4921 ttcttgcttt gaatcatcaa aagtttgtca ccaaaccaga tgattcccct gaatgcaaaa
     4981 cgatgcaaag tcgaaccgga caaattaatg tccggctgac ggtagatttt gaatatgatt
     5041 ctcttcattt taattgaagt acgctttcac cagattgatg gccattttga catcggccat
     5101 cgaaacgtcg ttgtgggtga cgaaccggaa cccgtcttcg taaggaagga tcttcactcc
     5161 gtgattgtaa agatattcct ccaacttgta ttttcgtttg tcgtcgatat cgaaaaacac
     5221 catgttgata tccctttggg tttcgtcgat cgcaacgcct ttgatctggt tcaaaagttc
     5281 cgccatgtat ttggcattct cgtggtcttc gttaaggcgt ttggtcatgc ggtcgaggga
     5341 gatcttgccg caagcggcaa gaatgccaac ctggcgcatg ccaccgccca tgattttacg
     5401 tttgatcctg gcttttttaa tgaaatcctt ggatccga
//
LOCUS       DAOPJL010000050         5152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_17496_length_5152_cov_5.362762, whole genome shotgun sequence.
ACCESSION   DAOPJL010000050 DAOPJL010000000
VERSION     DAOPJL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17496_length_5152_cov_5.362762"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PLP02_05625"
     CDS             <1..1279
                     /locus_tag="PLP02_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42955.1"
                     /translation="FISIVIAPIILFIAIMYFRSVEKQFQTIEANEAKMTTHVQENVS
                     GARVVKAFANEAYEERKFLKLSRTYTDSDFKLTKKMAVFWSGTDILCFIQFCLIGIFG
                     IIYTSKGIITIGVYTTFLAYAGNIIWPMRQLGRLMGDMSKATVAVARMENILGKEDEY
                     KGDSGILTPEITGKVKFDHVSFKFADSTYNQLQDISFDVKKGDTIAIVGKTGSGKSTL
                     MNLMVRLLDYQSGAIYLDDTPIKEIEKHYLRQHVGIILQEPFLYSKTVEENIGIADRA
                     IEDERVKEVAQIAKIHDDIVRFEKGYDTLVGERGVTLSGGQKQRVAIARMLLKPKPIL
                     IFDDSLSAVDTETDIQIRNALKKEWKDSTVFIITHRITTAMEADRILVLDQGIVKESG
                     NHKELLKTNGLYKKIWDIQSRIDFQLEGGEDHE"
     gene            1272..3122
                     /locus_tag="PLP02_05630"
     CDS             1272..3122
                     /locus_tag="PLP02_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42956.1"
                     /translation="MSDQLLDEENYTSNKIDWSVWKKIFSLMKPLKLFVFLSIASMAF
                     VAASDVIYPYISKYAIDNVITPNLGSGTVDMQGLKTLIMYYAIFMVVSAVMVFLFILF
                     AGKVQIELAYNIRKKAFHHLQELPFSYYDRTRVGWIMARMTSDSRNLSDILSWGLIDL
                     TWGVFVMIILIFVMFLINWKLALIAIAIIPFLAVISHYFRRYILKAHRSIRKVNSKIT
                     GLYNEGLTGAITTKTLVLEDSNFRDFDNLTSDMRKQSVRAAMIQGMYFPAVLFIISIM
                     ISIVYYFGGRMVSGDIITIGTLYLFTSYVWLFFDPINNIASVFARFQQAQASAERIVS
                     LIETPVDITDTPEVVEKYGTLFEYNRENFESLIGNVEFKNVSFKYQVGEKVLTDFNLS
                     VKAGESIALVGHTGAGKSTIINLLCRFYEPTGGEILIDGVDYRQRSLGWLHSNLGYVL
                     QTPHLFSGSVMENIRYGKLDATDEEVINAAKAVEAHEFIMRFEKGYDTECGEGGSRLS
                     VGQKQLVSFARAILADPRILVLDEATSSVDTETEKTIQTAINKLLEGRTSFIVAHRLS
                     TIVHSDRILVVEDGEIVEAGNHRDLIAKKGFYYKLYTNQYTEEMLELSKH"
     gene            3209..3295
                     /locus_tag="PLP02_05635"
     tRNA            3209..3295
                     /locus_tag="PLP02_05635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3243..3245,aa:Leu,seq:caa)
     gene            3440..4393
                     /locus_tag="PLP02_05640"
     CDS             3440..4393
                     /locus_tag="PLP02_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42957.1"
                     /translation="MVDNDVIKKYCLKGDVTGLIAWLAQNDEEGSVYKKYRSRFVFNE
                     EEIFVPDNDKLNKILCLYANYYKDVFYYKADNKMAIKWLGRSLSRFLGQKGGIKKAEK
                     KIKLLFEANGYHFLGGITSSYYGPYIWKQTEEKKYVVEIPDDTVELPVFFMRDWISNS
                     WLSYISFEVTGTGGWAKKDGLYCNYNTYEPLIDTDRFKISFLKHESQHFKDNRNKKRH
                     FTSQELEYRAKLCELSYLKDSSTLKKFINGAVNDERFTHGIAEYWVISDLSKAIFGED
                     YVSDISRWDFVYGQVAPEARNLLLKNNRNKKAIIPFLGSHK"
     gene            4619..>5152
                     /locus_tag="PLP02_05645"
     CDS             4619..>5152
                     /locus_tag="PLP02_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42958.1"
                     /translation="MDKAKKIGIFFSPYYLASVSLATLALWYFHLENYGIPVFLGLMF
                     MIFILFEDVVPAVPLLLNALFMVSNTDLTFANIPLYIYLTPVVLIVGIIVHVIRFKVR
                     FFRGKMTLGIGIMFLAVLLSTINAEFVDINYIFYMVIGVLYAFLYFFFNNSMKGDHVN
                     YLILMMFILGLLVSAETL"
BASE COUNT         1452 a         1099 c         1186 g         1415 t
ORIGIN      
        1 gttcatatcc atagtgatcg caccgattat ccttttcatc gccatcatgt attttcgcag
       61 cgtcgagaaa cagttccaga caatcgaagc taatgaagcc aagatgacga cccatgttca
      121 ggaaaacgtc agcggcgccc gggttgtcaa ggcgtttgcc aacgaagcct acgaggaaag
      181 aaaatttctc aaactaagcc gcacatatac cgattcggat ttcaaactga cgaaaaaaat
      241 ggcggttttc tggagcggaa ccgatattct gtgtttcatc caattctgcc tgatcggtat
      301 ttttggaatc atctatacat ccaaaggcat catcaccatc ggtgtttaca cgaccttcct
      361 tgcctatgcc ggaaacatca tctggccaat gcgccagttg gggcgtctga tgggtgatat
      421 gtccaaagcc acggtcgccg tcgccagaat ggagaacatt ctgggaaaag aagacgaata
      481 caagggagat agtggaatcc tgacgccgga gattaccgga aaagtcaagt tcgatcatgt
      541 ttcctttaaa tttgccgatt cgacctataa tcagctccaa gacatcagct ttgatgtcaa
      601 aaaaggcgat acgatcgcca ttgtcggaaa aaccggcagc ggcaagtcga ccttaatgaa
      661 cctaatggtc cgcctgcttg attaccagag cggggctatc tacctggatg acactccgat
      721 caaagaaata gaaaagcatt atctgcgcca acatgtcgga atcatccttc aggaaccgtt
      781 cctttactcc aagacagtcg aggaaaatat cggtattgcc gatcgagcga ttgaagacga
      841 aagggtcaaa gaagtggccc aaatcgccaa aatccacgat gatatcgtca gattcgaaaa
      901 aggttacgac accctggtcg gtgaacgcgg agtcactctg tccggtggcc agaaacaaag
      961 ggttgccatt gccagaatgc tgttgaagcc gaaaccgatt ctgattttcg acgacagttt
     1021 gtccgccgtc gataccgaaa ccgacatcca aatcaggaac gccctgaaaa aggagtggaa
     1081 agattccact gttttcatca tcactcatcg aatcacgacc gcaatggaag ccgatcggat
     1141 cctggtgctc gaccagggca ttgtcaagga atccggcaat cataaggagc ttctgaaaac
     1201 caacggtctt tataagaaga tttgggacat ccagtcccgg atcgactttc aattagaagg
     1261 aggtgaggac catgagtgac caactcctcg atgaagagaa ttatacgtcg aataaaatcg
     1321 actggagcgt atggaaaaag atattttccc ttatgaaacc gctcaaactg tttgtatttc
     1381 tgagcattgc ctcaatggct ttcgtggctg cctcggatgt aatttatcca tatatttcca
     1441 agtatgccat cgataatgtc atcacaccga atctcggtag tggaacagtg gacatgcagg
     1501 ggctaaaaac actgatcatg tattatgcga tcttcatggt tgtttcggct gttatggttt
     1561 tcctgtttat cctgttcgcc ggcaaggttc agatcgaact ggcttacaat atccgaaaaa
     1621 aagcatttca tcacttgcaa gaattgccgt tctcgtatta cgaccgcacg agagtcggtt
     1681 ggatcatggc caggatgact tcggattccc gcaatctaag cgatatcctg tcctggggcc
     1741 tcattgatct tacctggggt gtgttcgtga tgatcattct gatattcgtc atgtttttga
     1801 tcaactggaa actggcgctt atcgccattg cgatcattcc tttcctagcg gtcatatccc
     1861 attatttccg ccgctatatt ttgaaagctc atcgttcgat tcgcaaagtg aattccaaaa
     1921 tcaccggact ttacaacgaa ggattgaccg gagccattac gacgaagacg ctggtgttgg
     1981 aagattccaa tttccgcgac tttgacaatc tgacatccga catgcgcaaa cagtccgtca
     2041 gggctgcgat gatccagggc atgtattttc ccgccgtact tttcatcatt tcgatcatga
     2101 tatcgatcgt ctattatttc ggcggtagaa tggtgagtgg cgatatcatc accatcggta
     2161 ctttgtatct gttcaccagt tatgtctggc tgtttttcga tcccataaac aacatcgctt
     2221 ctgttttcgc ccgctttcag caggcccagg cttcggccga acggattgtg tctttgatcg
     2281 agacgccggt tgacatcacc gacacgcccg aagtcgttga aaaatacggc actctgttcg
     2341 agtacaatcg ggagaatttc gaatcgctca tcggcaatgt cgaattcaaa aacgtttcat
     2401 tcaaatatca ggtcggagag aaagtgctaa ccgacttcaa ccttagtgta aaagccggag
     2461 agtcaatcgc tctcgtcggg cataccggcg ccggcaaatc gaccattatc aatcttttat
     2521 gccgtttcta cgaaccgacc ggcggcgaga tcctgatcga cggggtcgac tatcgccaaa
     2581 ggagcctcgg ctggttgcat tcaaacctcg gttacgtgct tcagacgccg catcttttct
     2641 ccggaagcgt gatggagaac atccgttacg gaaagttgga tgcgacggat gaggaagtaa
     2701 tcaacgcggc aaaagccgtc gaagcgcacg aattcatcat gcggtttgaa aaaggttacg
     2761 acaccgaatg cggtgaaggt ggttcaagat tgtccgtcgg ccagaaacag cttgtttcat
     2821 tcgcccgggc gatccttgcc gatccgcgga tattggtgct ggacgaagcg acatcctcag
     2881 tggataccga gacggaaaaa acgatccaaa ccgccatcaa caagcttctc gagggacgga
     2941 cgtcgtttat cgtcgcccac cggttgtcga ctatcgttca cagtgacagg atcctggtag
     3001 tggaagacgg agagatcgtc gaagccggaa accaccgcga tcttatcgct aaaaaaggat
     3061 tttattacaa actgtatacg aaccagtata cggaagaaat gttggaattg tccaagcatt
     3121 aaggcggccg gcctattggc cgcctttttt attaaataaa aaaattggtt tgatttccag
     3181 aaaaatagtg tataatattt attgctatgc cggtgtggtg aaattggtag acacgataga
     3241 ctcaaaatct attgccagta atggtgtgcc ggttcgagtc cggccaccgg taccagagaa
     3301 aaaagaagcg atgtaaatcg ctttttttgt tgcattcaag tccgaaaaac aagcttgcaa
     3361 ccgttggttg tatatgctta ggatggattg acttgccaat tgtaaaatga tacgattttc
     3421 tggtaagggg gatcacaaca tggtcgacaa tgatgtaatc aagaaatact gtttgaaagg
     3481 cgatgtcacc ggcttaattg cctggctggc tcaaaacgac gaagagggaa gcgtgtataa
     3541 aaaatatcgt tcccggtttg tatttaatga agaagaaata ttcgtgcctg ataacgataa
     3601 attgaataaa attctatgtt tgtatgccaa ctattacaaa gatgtgtttt attacaaagc
     3661 tgataataaa atggcaatta aatggctcgg tagaagtctt tcacgttttc tcgggcaaaa
     3721 aggggggatc aaaaaagcgg aaaaaaagat caaactgctt tttgaagcga atggttatca
     3781 ctttctcgga ggaataactt cttcctatta cggaccgtat atttggaaac agacggaaga
     3841 aaagaaatat gtcgtcgaga ttcccgacga cacggtcgaa ttgccggtgt ttttcatgcg
     3901 cgactggatt tctaacagct ggttgtcata tatttccttt gaagtgacgg gaacaggcgg
     3961 ctgggccaaa aaggatggtc tttattgtaa ttacaacact tatgagccat tgattgatac
     4021 cgatcgtttc aaaatatctt ttttgaagca cgagtcgcag cactttaaag acaacaggaa
     4081 caaaaagaga cattttacat cccaagaact ggaatatcgt gccaaactct gcgaattgtc
     4141 atatttaaaa gattcttcca cactgaaaaa attcatcaac ggcgccgtca atgacgagag
     4201 attcactcat ggaattgccg aatattgggt gatctccgat ttgtcaaaag cgattttcgg
     4261 tgaagattat gtttccgata tttcccgctg ggattttgtg tatggccaag tggctccgga
     4321 agccagaaat ttattactga aaaataatcg taacaaaaaa gcaattattc ctttcctcgg
     4381 atcacataag taatataggc tgtgcgtttt caaaagataa tttctttgta aattattgtt
     4441 attgcataac aagcggcaaa cagccagcac agcctgaccg ggatgaaaat cccggttttc
     4501 ttttataaaa agaaaaatta tgcaaaacgg gcggaaaaca ttttcatttt attacaagtc
     4561 ctttgccata tgaattatgg tataatatat tgacaacttc aaagaaagga aaaagataat
     4621 ggataaagcc aaaaaaatcg ggatattttt cagcccttat tacttggcct cagtcagttt
     4681 ggcgactttg gctctttggt attttcatct tgagaattac gggattccgg tttttcttgg
     4741 attgatgttt atgattttca ttctgtttga ggatgtcgtg ccggcggttc ctttgctttt
     4801 gaacgcccta ttcatggtaa gcaatactga tttgacattt gcgaacattc cgctgtatat
     4861 atacttgact cccgttgttt tgattgtcgg cattattgtg cacgtgatcc ggttcaaggt
     4921 gcggtttttc cggggaaaga tgactttggg aatcgggata atgtttctgg cagtgttatt
     4981 gtcgaccatc aatgccgaat tcgtcgacat caattatata ttttacatgg tgatcggggt
     5041 tctttacgca ttcctgtact ttttcttcaa taattcgatg aaaggcgacc atgtgaatta
     5101 tctgattctg atgatgttca ttcttggtct tttggtatcg gcggaaacat tg
//
LOCUS       DAOPJL010000051         5002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_18219_length_5002_cov_9.474227, whole genome shotgun sequence.
ACCESSION   DAOPJL010000051 DAOPJL010000000
VERSION     DAOPJL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18219_length_5002_cov_9.474227"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLP02_05650"
     CDS             complement(<1..755)
                     /locus_tag="PLP02_05650"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPF42959.1"
                     /translation="MVKLFQVIHMGNKTIVGQATANGISALHLIRVSGDDAFSVVGKI
                     FNGPSLDKALHHSVIYGHIEENGEIIDEVMVFVYRAPNTYTRENMVEISCHGGFLAVS
                     EIIRLLVKSGADLAKKGEFTKRAFLNGRIDLTEAESVMDIVNAKTRSQLVLAEKSLGG
                     EIKSLIAGLQSDLLNIIAKIEVNIDYPEYDDIEILTNETLVPEISALKTKIDKIISQS
                     KTGKIIREGVSVVIAGKPNVGKSSLMNSLLKEE"
     gene            800..1279
                     /locus_tag="PLP02_05655"
     CDS             800..1279
                     /locus_tag="PLP02_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42960.1"
                     /translation="MENRKKPFKFYLTIFLMSTFVVLAYSLYMIISGKVEAKDMISFW
                     FLPPIFTALYYGSDVLRDKIYKKRRKPDPEKEFLEKSSKRLRESGEFLIEDFRRLQGS
                     QKFQEAIKTAFQITQTGEDDTWTLDKLDKKFRPDTTEGKAMSYIVAYAREQLNSKDK"
     gene            1360..3177
                     /gene="dnaX"
                     /locus_tag="PLP02_05660"
     CDS             1360..3177
                     /gene="dnaX"
                     /locus_tag="PLP02_05660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPF42961.1"
                     /translation="MSYKALYRTYRPVDFNEVAGQKHITITLQNALQNNKVAHAYLFS
                     GPRGTGKTSIAKIFAKAINCEQAPTANPCNVCPNCIGIQNGTISDIVEIDAASNNGVD
                     EIREIRDKVKYLPGYVKYKVYIIDEVHMLSAGAFNALLKTLEEPPQHVIFILCTTEPQ
                     KIPQTIHSRCQRFDFKAISVADIAGKLDEIIEKESIKIDKDAINQIAVFAEGGLRDAI
                     SLLDQVNAYSPDHITMEDVNQICGAISQESQIELVKSIVEMESTGAIQSMNDLVSQGK
                     EIHKIALNLMDFFRAMLMYKNIGPIGDANSLYKNEEFVQLTKIVSNRRIFFYIDVLQK
                     AINEMKWSTSPKLYLELAFIKMTDKEVSSDAKLLESIDSLEKRIRIIETAKPKTPEPE
                     KIVLPAKEEKPSLDIFMEEPEEKQIEDLPIEEPEKEETPDDLPEEDIANTYRIEFVES
                     VLNNGSRDDKNYLASNWNRLTKFNSHGNLKQYAAQFETGTVRASSNDALIVTFSTAGM
                     CNQMMKPAVKKSIKEVLKRAFDRDIDYIALPDAVFEDIAKEFAEQWHQGRRDITLSPI
                     VCDGLRDVSGEQGEDESEKEEKVITEAVNIFGDLVTIKK"
     gene            3197..3505
                     /locus_tag="PLP02_05665"
     CDS             3197..3505
                     /locus_tag="PLP02_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPF42962.1"
                     /translation="MINPQMIKKIQKMQEDLKKAQAEINASTFYGQAGGSVVKVAVKG
                     NKEVISIDIKKDEIDLTDIEMVQDMIVAALNDAFKKVDKELEETIGSITQGMQIPGLF
                     "
     gene            3505..4107
                     /gene="recR"
                     /locus_tag="PLP02_05670"
     CDS             3505..4107
                     /gene="recR"
                     /locus_tag="PLP02_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPF42963.1"
                     /translation="MSYPKAMEDLIADFMKYPGIGRKTAERYAIFTIDKITQEDLDGF
                     ASDISKIKQDVFPCPVCGQLTDINPCQVCSDPKRDKSVIMIVESPKDAFSFERGGTYN
                     GLYHVLHGVISPMDGTGPEDINLSSLWPRVQEKKIKEIIIATSATQEGEATAMYIQRA
                     LKNLDILTTRIGYGVPVGGNLDYADDQTLTKAVENRKAYR"
     gene            4227..4550
                     /locus_tag="PLP02_05675"
     CDS             4227..4550
                     /locus_tag="PLP02_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42964.1"
                     /translation="MNVYAMTIIDTINSYIQPFINQAISFFQGLYIWAQGLILLALAI
                     FAIIGLVVFIKKFIKLFLVLGVLAVIVYFVWTKTTIIQDLIGNFAGASIVPKNIVATI
                     LSMPF"
     gene            4719..>5002
                     /locus_tag="PLP02_05680"
     CDS             4719..>5002
                     /locus_tag="PLP02_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPF42965.1"
                     /translation="MKKRMFLSVLSILFLTFAFIGCQTQQTTYTLPDLSGMTETEARE
                     ELSSLLVTIVVKTEQNYNVETGDFSRYGNDLAAGSEITPGTVLTLYFAENP"
BASE COUNT         1625 a         1048 c         1085 g         1244 t
ORIGIN      
        1 ttttcttctt tcagcaagga gttcatcaaa cttgattttc cgacgttggg ttttcctgca
       61 atgacgacac tgactccttc gcgtatgatt ttgccggttt tggattgaga aatgatttta
      121 tcgatttttg tctttagagc ggaaatttcc ggcacaagag tttcgttggt gagaatttcg
      181 atatcgtcat attccgggta atcaatattg acttcgattt ttgcgatgat gttcaagaga
      241 tcgctttgca aaccggcaat cagggatttt atttcaccgc caagagattt ttcagccaga
      301 acaagttgag atctggtttt ggcattcacg atatccatca cgctttccgc ttcggtaagg
      361 tcgatgcgcc cattaagaaa agctcttttg gtgaattcgc ctttttttgc cagatcggcc
      421 cccgatttga ctagcagtct gattatttcc gacacggcga gaaacccgcc gtggcaggat
      481 atttcgacca tgttttccct tgtatatgta ttaggagccc ggtaaacgaa aaccatgact
      541 tcgtcaatga tctctccgtt ttcttcgata tgcccgtata taacggaatg atgaagcgct
      601 ttatcgaggc ttgggccatt aaatatcttg cctacaacag aaaaagcatc gtctcccgat
      661 acccttatta aatgtaacgc agaaatgcca ttggcagtcg cctgcccaac aatcgttttg
      721 tttcccatat ggatcacctg aaataatttt accatatttg tgccatcata ttgtataata
      781 cttttaaagg tgatagacaa tggaaaacag gaaaaaaccg tttaaatttt atttgaccat
      841 ctttttgatg tcgactttcg ttgtgcttgc ttattcgctt tatatgataa tatccggcaa
      901 agtggaagcc aaggacatga taagtttctg gtttttgccc ccgatattca cggcacttta
      961 ttacggaagc gatgtattaa gggacaagat ttacaaaaaa agacgcaaac cggatccgga
     1021 aaaagaattt ttggaaaaat ccagcaaaag actccgggaa tccggagaat ttctgatcga
     1081 ggattttcgc aggttgcagg ggagccaaaa attccaggaa gccataaaaa ccgcatttca
     1141 gattacccaa accggggaag acgatacttg gacgcttgat aaattggata aaaagtttcg
     1201 tccggacacc acggaaggaa aagcgatgtc ttatatcgtc gcatatgcta gggaacaatt
     1261 gaattccaaa gacaaataaa cggaatgcca tgtaaaaaac caacgaaatg agatataata
     1321 aatctgtaat aaacaaacaa agaaaggtta tggtgcataa tgagttataa ggcgctttat
     1381 agaacataca gacccgtcga tttcaatgaa gtcgccgggc aaaaacacat aaccataact
     1441 ttgcaaaacg cgttacaaaa caacaaagtc gcacacgctt atctttttag cgggccgcgc
     1501 ggaaccggaa aaacgtcaat agccaaaatt tttgccaaag ccatcaactg cgaacaagct
     1561 ccgacggcca atccgtgcaa tgtttgccct aattgcatcg gcatccagaa cgggacaata
     1621 agcgacatcg tcgagataga tgccgccagc aacaacggcg tcgacgagat ccgggaaatt
     1681 cgcgacaaag taaaatattt acctggatac gtcaaataca aagtgtatat aatcgatgaa
     1741 gtccatatgc tgtcggccgg cgctttcaac gcgcttctca aaactttgga agaaccgcca
     1801 caacatgtga tttttattct ttgtaccacc gaaccgcaaa aaattccgca aacaatccat
     1861 tcccgttgcc aacgattcga tttcaaagcc atatcggtcg ctgatatagc cggtaaattg
     1921 gatgaaataa tagaaaaaga aagcataaaa atcgataagg acgccattaa ccaaattgcc
     1981 gtgttcgcag aaggcggatt gcgtgacgcc atcagtcttc tggatcaggt aaacgcctac
     2041 agccccgatc acataacaat ggaagatgtc aatcaaatat gcggcgccat atcccaggag
     2101 agtcagatcg agcttgtaaa atccattgtg gagatggaat cgaccggagc gatccaatca
     2161 atgaatgatt tggtatcgca aggcaaggaa attcacaaaa tcgccttgaa tttgatggat
     2221 tttttccgtg cgatgctgat gtataagaat atagggccga tcggagatgc caacagtctt
     2281 tataaaaacg aagaattcgt tcaattgacc aagattgtca gtaatcggag aatctttttc
     2341 tatatcgacg tgttgcaaaa agccataaac gaaatgaaat ggagcacaag ccccaaatta
     2401 tatcttgaac tggcttttat caagatgacg gacaaagagg tttccagcga cgccaaattg
     2461 ctggaaagca tcgattcttt ggaaaaacgg ataagaatca tcgaaaccgc aaaacccaag
     2521 acgccagaac cggaaaaaat cgttttgccc gcaaaagaag aaaaaccgag tctcgatatt
     2581 tttatggaag agcccgaaga aaaacaaata gaagatctac ctatagaaga accggaaaaa
     2641 gaagaaacgc cagatgacct tccggaagaa gatattgcca atacttacag gatcgaattc
     2701 gtggaaagcg ttctgaacaa cggaagcaga gacgacaaga attatctggc aagcaactgg
     2761 aaccgactga caaagttcaa ttcccacggc aacctgaaac aatatgccgc tcaattcgag
     2821 accggaacag tgagagcgag ctccaatgac gcgcttatcg tcacgttttc cactgcagga
     2881 atgtgcaatc aaatgatgaa accggcggtg aaaaaaagca tcaaagaagt tctgaaaagg
     2941 gctttcgaca gagacatcga ttatatcgct ttgccggatg ccgtgtttga ggacatcgcc
     3001 aaggaatttg ccgaacaatg gcaccagggc cgtcgggaca taactttgtc tcctatcgta
     3061 tgcgacgggc tcagggacgt ttccggagag caaggggaag acgaatcgga aaaggaagaa
     3121 aaagtaataa ccgaagctgt taatattttc ggtgatctag tgacaataaa aaaataacat
     3181 tttgggagtg acggaaatga tcaatccgca aatgatcaaa aaaattcaaa aaatgcagga
     3241 agatctgaaa aaagcccaag cagagataaa tgcatcgaca ttttacgggc aagcgggcgg
     3301 ctcggtggtc aaagtcgccg tcaagggaaa caaagaagta atatcgatcg acataaaaaa
     3361 agacgaaata gatcttacag atatcgagat ggttcaggac atgatagtgg ccgctttgaa
     3421 cgatgcattc aaaaaggtcg acaaagaact tgaggaaact attggatcca taacacaggg
     3481 aatgcaaatt ccggggcttt tctgatgagt tatcccaagg cgatggaaga tctgattgcc
     3541 gatttcatga agtatccggg aatcggcaga aaaaccgccg aaaggtatgc cattttcacg
     3601 atcgacaaaa tcacacagga agatttggat ggattcgcat cggatatttc caagataaaa
     3661 caggacgttt ttccttgccc cgtatgcggt caactgacgg atataaaccc gtgtcaagta
     3721 tgttctgacc caaagcgaga caaatcagtt attatgatcg tggaatcgcc aaaggacgcg
     3781 ttttcctttg aacgcggcgg gacgtataac ggattatatc acgtgcttca cggagtgatt
     3841 tcgccaatgg acggaacggg accggaagac ataaacctgt ccagtctctg gccgcgggtc
     3901 caggagaaga aaataaagga aatcatcata gccacatcag ccactcagga aggcgaagct
     3961 accgccatgt acattcagag agccctaaaa aacttggaca ttctcacaac aagaatcggc
     4021 tatggcgtac ccgtcggcgg gaaccttgat tatgctgatg atcagacctt gacaaaagcg
     4081 gtggaaaacc gaaaagctta ccgataaata tttttgttcg acaatttgaa aaaaatagaa
     4141 aaacgctctt ccttccgaaa atatttatga tataatgttt atacaacata atataaaaga
     4201 tttatccata aaaggggtgg aaacaaatga atgtttacgc tatgacgatt atcgacacaa
     4261 tcaacagcta catccaaccg ttcatcaacc aggctataag ctttttccag ggcctgtaca
     4321 tttgggcgca aggactcatt ttgttggcgc ttgccatttt tgccataatt ggtctcgtgg
     4381 tgtttatcaa gaaattcata aaacttttcc tggtccttgg tgtattggcc gtcatagtgt
     4441 acttcgtttg gactaaaaca acaataatcc aagaccttat aggcaatttt gcgggagcct
     4501 caatcgttcc caagaacata gtcgccacaa tattaagtat gcctttttaa cgaaaggcat
     4561 atttttttct atcttgcttc catttcaaag cgttgacatg ataaaaagat caaaccgaat
     4621 atcaaagccc gattctcttg atctcaagga cgctttcaga acgaaagcgg cctttttata
     4681 gagcacagga tattgaacaa acaaggagcc cgatcgacat gaaaaaaaga atgtttcttt
     4741 ccgttttatc aattttgttt ctgacatttg cgttcatagg atgccaaacc cagcaaacca
     4801 catatacatt gcccgaccta tccggcatga ccgaaaccga ggcaagggaa gagttatctt
     4861 ccttgcttgt gacgatagtc gtcaaaacgg aacagaacta caatgtggaa actggtgatt
     4921 tttcgcgtta cggcaatgat ctggctgccg gcagcgaaat aactcccgga acagtattga
     4981 cgctttattt tgcagaaaat cc
//
LOCUS       DAOPJL010000052         4745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_19562_length_4745_cov_5.794243, whole genome shotgun sequence.
ACCESSION   DAOPJL010000052 DAOPJL010000000
VERSION     DAOPJL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19562_length_4745_cov_5.794243"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1789)
                     /locus_tag="PLP02_05685"
     CDS             complement(176..1789)
                     /locus_tag="PLP02_05685"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42966.1"
                     /translation="MKQYLILMRVFLKENFSLKRFLGIDVRKSKKKAILLGFLFLYAI
                     GAWWFFFGLLFMDLAEVFDSLGVIELMLMYLFLYTTGMTLIMVLFRANGYLFKYKDFE
                     ILQPLPLKPNTVFMAKMTVMLIITYIGVFILAAPIAFAYFYYDFSIIGLLLFLIALPL
                     MCLIPIVVFSFLSMIVARLASGFRNRNILNIISMLLLFLCIMAFSLWLNISTANDVNP
                     LLNQQGLVEGMKEYYLPMGWFADAIHNHDILSFLLLFVSNTVPFALFVYLVAKWTVKI
                     NQKNMSVKTRTSVKTAVSQSQPVFQAIIRKEFRKFLSVPIYAVNAGFGLLMLMIAGIL
                     SVVYKAKIAEFMATITSSTITPEAIALLFVGFCITMVYTSAISLSLEGKNFWIIKSLP
                     LEAKKVMEAKMAFNVLLVMPFAIVSLVLIYFGIGSTIINLLVMFLYTLSLSLASSAFG
                     SFINLHFPKFEFLNETEVVKQSVGAMIGVFSGFGFLVISGLVYYWLADTIGSMGALCA
                     ASLINYVFFFVFLQIVDRTSESLFAKMSG"
     gene            complement(1786..2499)
                     /locus_tag="PLP02_05690"
     CDS             complement(1786..2499)
                     /locus_tag="PLP02_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42967.1"
                     /translation="MLEIIGLSKSYDGVKLACDDITLTIENGDIYGFIGHNGAGKTTL
                     LKSIAGIIDLEKGDVLIGGKSIKTNPIAAKRQIAYIPDNPDVYESLTGMQYIDFMADV
                     FEVAPDRRKELVEKYAKMFEMDSVLGNPIQSYSHGMKQKIVIMGALIHEPKVMLLDEP
                     FVGLDPKASYLLKEVFREMCDKGALIFFSTHVLEVAEKLCNKVAIIKQGKIVENGITQ
                     EIIKKESLEQIFLELLNEA"
     gene            2611..2684
                     /locus_tag="PLP02_05695"
     tRNA            2611..2684
                     /locus_tag="PLP02_05695"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2643..2645,aa:Thr,seq:ggt)
     gene            complement(2715..3950)
                     /locus_tag="PLP02_05700"
     CDS             complement(2715..3950)
                     /locus_tag="PLP02_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPF42968.1"
                     /translation="MKVLALILAGGRGSRLDILSENRVKPSVPFAGKYRIIDFTLSNC
                     SNSGIYNIAILTQYLPFSLNEHIGSGRPWDLDRRDSKVTLLQPHSQWYMGTADAVRKN
                     LHYIEQSDPDYVLILSGDHIYKMDYRDMINQHIMTGADLTVGCKIIDPKDAFRFGMLE
                     ADQSGKIYKFQEKPKQTTSDLASMGIYVFNTKTLLDQLKNNDTPDLDFGKHIIPGMIE
                     KNRVYAFRFNSYWKDVGTYDAYLEANLELIETVDKIQLDMYDTNWKIYTKSEELPAVK
                     IGSKAVIRQTLLSNGAIVAGNVERSVLSPGVIVHPLAKVTNSVLLNYVEVKPGAIVDH
                     CIIDKNTVIGDNAFVGFGDDLTPNKENPDLLASGITVLGKNIMVPKNMVIGRNCRIFK
                     TADLKNIEDNIVPSGSTLK"
     gene            complement(3947..>4745)
                     /locus_tag="PLP02_05705"
     CDS             complement(3947..>4745)
                     /locus_tag="PLP02_05705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF42969.1"
                     /translation="KEIMTDYYGYGMQRLLKARAGDVKGILNGIDMKEFNPVTDKLIA
                     EKYGIDDYREGKIANRKALEDAITAHLPAGRPLVGIVSRLVSQKGIDLIKRVFDEMLE
                     TDDFSCVVLGNGQAEYEDYFKKLALDHPDRVFVRIGYDNKLAHLIYAASDIFLMPSKF
                     EPCGLGQMIAMRYGSIPVVRETGGLSDTIQPFNEYVPSGNGFSFTNFNAHDMMHVLRY
                     AFRLYRLQGVWNYLVKEAMSVDFSWQKSAKEYKKVYKQLLKNKEETI"
BASE COUNT         1339 a         1050 c         1034 g         1322 t
ORIGIN      
        1 tttcctccga gaaggttccg catggaagcg tcgccttcct taaacaaata aacgtacttt
       61 gtcatattat tatcctttcc atatagtttt tatatcctat agaattatat catatatata
      121 ttgccaaata caaacaaaag cgccgggcct ttgactacga aagcgttttt atttttcatc
      181 ccgacatttt agcgaaaagc gattcggatg ttctgtcgac tatctgcagg aaaacaaaga
      241 aaaacacata gtttataaga cttgcggcgc ataaggcgcc catggatccg atagtatcgg
      301 ctaaccagta ataaacgagc cctgatatga ctaaaaaccc gaatccggaa aacactccga
      361 tcatcgcgcc gacgctttgc ttgacaactt ccgtctcgtt cagaaattcg aatttcggga
      421 aatgcagatt gataaatgat ccgaaagccg aggatgcaag cgataacgac agggtgtaaa
      481 ggaacatcac caaaaggttg atgatggtcg atccgatccc gaaataaatc aatacgagag
      541 aaacgatggc aaaaggcatc accagcaaaa cattgaaggc catcttggct tccatgactt
      601 tcttcgcttc caaaggcaga cttttgatga tccaaaaatt ctttccttcc aaagaaaggc
      661 tgatagcaga cgtatatacc atggtgatgc aaaatcccac aaacagaagt gcgatcgctt
      721 caggagttat tgtactcgaa gtgatcgtag ccatgaattc ggcgatcttg gctttgtata
      781 cgaccgacag aataccggca atcataagca tcaataatcc gaagccggcg ttaaccgcgt
      841 aaataggcac gctgaggaac tttctgaact ctttccttat gattgcctgg aaaacgggct
      901 gagactgtga aacggccgtt tttacgcttg tcctggtctt tacggacata tttttctggt
      961 ttatcttgac cgtccatttt gccaccaaat aaacgaacag ggcgaatgga accgtattgg
     1021 agacgaaaag caataagaat gacaaaatgt catgattatg gatagcatcc gcaaaccatc
     1081 ccatcggcag atagtattcc ttcattcctt cgacgagtcc ctgttggttt aatagaggat
     1141 tgacatcatt tgccgtagaa atgttgagcc acagggaaaa cgccatgatg caaaggaaaa
     1201 gcaaaagcat gctgatgatg ttcagaatgt tgcggttacg gaaaccggat gcaagtctgg
     1261 caacgatcat ggatagaaag gaaaatacca caatcgggat cagacacatc agaggtaaag
     1321 caatcaagaa caataaaaga ccgataatgg aaaaatcgta atagaaataa gcaaaagcaa
     1381 tcggagccgc caaaatgaag acaccgatat aagtaattat aagcatgacc gtcattttgg
     1441 ccatgaaaac cgtgttaggt tttaggggga gcggttgcaa aatctcgaaa tctttgtatt
     1501 tgaacagata tccgttggcc cggaacaaaa ccatgatcag agtcattccg gttgtgtaga
     1561 gaaacagata catgagcata agctcgataa cacccaaaga atcgaaaact tcggcaagat
     1621 ccatgaagag aagcccgaag aaaaaccacc aagcgccgat ggcataaagg aacagaaagc
     1681 cgagcagaat ggctttcttt ttcgatttcc tgacatcgat gcccaaaaac cgtttaagcg
     1741 aaaagttttc cttgaggaaa acccgcatca agatgagata ctgtttcatg cttcgttcaa
     1801 cagttcaagg aagatctgct ctagtgattc ctttttgata atttcctgag tgatgccgtt
     1861 ctcaactatt tttccttgtt tgatgatggc taccttgttg cagagtttct ctgcgacttc
     1921 gagaacatgg gttgaaaaga agatcaaagc tcccttgtcg cacatctcgc gaaatacttc
     1981 cttgagcaga taactggctt tcggatcgag accgacaaac ggttcgtcta ataacattac
     2041 cttcggttca tggattaagg cccccatgat gacgatcttc tgcttcattc cgtgcgaata
     2101 agactggatc ggatttccga gcaccgaatc catttcaaac atcttcgcat atttttccac
     2161 aagttccttg cgcctgtcgg gagcgacttc gaagacatct gccatgaaat cgatatattg
     2221 catgccggta agtgattcat agacatcggg attatcggga atataggcaa tttgcctttt
     2281 tgccgcaatc gggtttgtct tgatcgattt accgccgatc aacacatcgc ctttctcgag
     2341 atcgatgatc cccgcaatcg atttcaacag cgtagttttt ccagcgccgt tatgaccgat
     2401 aaatccgtag atatcaccgt tttcgatcgt cagtgtaatg tcgtcgcaag ccagtttgac
     2461 accgtcgtag cttttagata agccaatgat ttccagcatg ttatttcctc caatgaataa
     2521 agtcatttat gtttataatg caactgattc gtttcgccaa caaaattctt gctttttcgg
     2581 aaaatcttgt ttataatatg aaaagcaagc gctcctatag tttaatggca aaacacagca
     2641 atggtaatgc tgaaattcta gttcgattct aggtaggagc accacggata atcaaaaggg
     2701 cagaacgccc ttttttattt tagtgtcgat cctgaaggaa caatgttgtc ttcgatattc
     2761 ttgagatccg ccgttttgaa gatccggcag tttcttccga tcaccatgtt tttcggaacc
     2821 atgatgtttt taccgagcac ggttattccg gaagctaaca aatcgggatt ttccttgttg
     2881 ggagtcagat cgtcgccgaa gccgacaaaa gcgttgtcgc cgatgaccgt gttcttatcg
     2941 atgatacagt gatcgacaat ggcacctggt ttcacttcta catagttcaa aagaacgctg
     3001 ttggtgactt tggcaagcgg atggacgatg acacccggtg aaaggacgct tctttccacg
     3061 tttccggcta cgatggctcc gtttgacaga agcgtctgtc tgatgacagc tttcgatccg
     3121 attttgacag cgggaagctc ttccgatttc gtatatattt tccagttggt atcgtacatg
     3181 tcgagttgaa tcttatcaac tgtctcgatc aattcaagat ttgcttccag ataagcgtca
     3241 taagttccga cgtctttcca gtaagaattg aacctgaagg cgtatactct gttcttttcg
     3301 atcattccgg gtatgatatg ttttccaaaa tccagatcgg gagtatcgtt gtttttcagc
     3361 tgatcaagca atgttttggt attgaaaaca tagattccca ttgatgcgag atcggatgta
     3421 gtctgtttcg gtttttcttg gaatttgtag attttacctg attgatcggc ttccagcatt
     3481 ccgaaacgga aagcgtcctt cggatcgatg atcttgcatc cgaccgttaa gtcggcgccg
     3541 gtcatgatat gctggttgat catgtcgcgg taatccatct tgtagatatg atcaccagaa
     3601 agaatcagaa cataatccgg atcactttgt tcgatgtaat ggaggttttt acgtacggca
     3661 tcggccgtac ccatatacca ctggctgtgc ggttgcaata acgtcacttt cgaatctctt
     3721 ctgtccaggt cccaaggcct tcccgaaccg atatgttcgt taagagagaa aggcagatac
     3781 tgcgtgagaa tggcgatgtt gtagatgccg gaattggaac agttagacaa tgtgaagtcg
     3841 atgatcctgt atttaccggc aaaagggaca gatggtttaa cccggttttc cgataggata
     3901 tcgagacgtg atccgcgacc tcccgccaaa atcaaagcaa gcactttcat atggtttcct
     3961 ccttgttttt taagagttgt ttatagactt tcttgtattc tttcgccgac ttctgccaac
     4021 tgaaatcgac ggacatcgct tctttgacca gatagttcca gacgccttgc aaccggtaaa
     4081 gacggaaagc gtagcgaagc acatgcatca tgtcatgagc attgaagttt gtgaaactga
     4141 atccgtttcc ggatgggaca tattcgttga atggttggat tgtatcggaa agcccgccag
     4201 tttcccttac taccggaata gagccatatc gcatggctat catctggccg agcccgcaag
     4261 gttcgaattt ggacggcatc aagaaaatgt cgctggcggc gtagattaga tgagccagtt
     4321 tattatcata tccgatccgc acaaaaaccc ggtccggatg atcaagtgcg agttttttga
     4381 agtaatcttc atactcagct tgtccgttac ccaaaaccac acaggaaaaa tcatcggttt
     4441 caagcatctc gtcgaacact cgcttgatca agtcaattcc tttttgactg acaaggcggc
     4501 tgacaatacc gaccaaaggt cgtccagccg gaagatgagc ggtaatcgca tcttccagcg
     4561 cttttcggtt ggctatcttt ccttcccggt aatcatcaat cccgtatttt tcagctatta
     4621 gtttatcggt aaccggattg aactccttca tatcgattcc gttcagaatg cctttgacat
     4681 cgccggctct ggcttttaga agtctttgca tcccataacc gtaataatcg gtcatgatct
     4741 ccttg
//
LOCUS       DAOPJL010000053         4424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_21543_length_4424_cov_7.999542, whole genome shotgun sequence.
ACCESSION   DAOPJL010000053 DAOPJL010000000
VERSION     DAOPJL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21543_length_4424_cov_7.999542"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLP02_05710"
     CDS             complement(<1..686)
                     /locus_tag="PLP02_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HPF42970.1"
                     /translation="MTKRFIDLRSDTVTKPTSDMLMAMTNAEVGDDVFSDDPTMNELE
                     AMAARMLGKDDAVFVPSGTFSNQLALFTHCVRGEEVIVDQDAHIVQHESGASSIISGV
                     QLFTMESYNGIWDLEKLAKTVKERTISTTETRLICIENAHGGSVLPLDYMKKVYDLAK
                     SKNLLVHLDGARIFNAATFLKCDVKEIAKYADSISVCLSKGLCSPIGTLLVGSKDFIK
                     KARIKRKIMGG"
     gene            complement(686..967)
                     /locus_tag="PLP02_05715"
     CDS             complement(686..967)
                     /locus_tag="PLP02_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPF42971.1"
                     /translation="METLKGYRDRIDDIDAKIRELFLERMTIVDQIAKAKMEKDMQIY
                     DSSREDQVKKKNLNAVKQEEYKEYYSEVLETILKVSKDFQRALIFRSAQ"
     gene            complement(1221..2090)
                     /locus_tag="PLP02_05720"
     CDS             complement(1221..2090)
                     /locus_tag="PLP02_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF42972.1"
                     /translation="MYDVIVVGGGPAGVSAAIYLKRLKLSVGIVMKGMGSLGKTGHIE
                     NYYGVPGPVSGADLFQKGLDQARALGIPIYQGEVLGIDNFDGFAVKTTEGTCETTCIA
                     KAVLLATGVNKINLRVKGFDGLVGHGISYCVICDGFFYRDKKIGLVGSGDFMKDELEN
                     LANISKDITIFTNGEKLTVDTGTYPVDSGKLTEIGGIDHLEYVATENGKYPLDALFVA
                     VGTPSATDFAVRMGAFTDHNNIVVDENYMTNIPGLFAAGDCIGGLLQIAKAVDDGAKA
                     GLAINKYLKSLKG"
     gene            complement(2094..2405)
                     /gene="trxA"
                     /locus_tag="PLP02_05725"
     CDS             complement(2094..2405)
                     /gene="trxA"
                     /locus_tag="PLP02_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPF42973.1"
                     /translation="MEVKITNDNFKKEVLESELPVIVDFWAAWCRPCMMLGPIIKELS
                     EDLEGKVKVGKVNVDEEGYLAQQFRVFSIPTVILFKEGKPAAQVVGLLRKEDLLKRLG
                     L"
     gene            complement(2477..2917)
                     /locus_tag="PLP02_05730"
     CDS             complement(2477..2917)
                     /locus_tag="PLP02_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF42974.1"
                     /translation="MNECNLVTDLIRGIAEYEKLSDQVEATPEKLYQALFVDKTCKAV
                     IGFENEKPVGFALYFYNFSTFKAKKGLYLEDLFIKEPYRHKGYGKQFFLYLIQEAKQE
                     NCGRMEWTCLDWNTKAIAFYNSLKAKPMDEWTVFRLDEKDFERF"
     gene            complement(2947..3531)
                     /locus_tag="PLP02_05735"
     CDS             complement(2947..3531)
                     /locus_tag="PLP02_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42975.1"
                     /translation="MLILLGPSASGKTESAKIMINRYPISRVVTCTTRPQRINEIDGF
                     DYHFMTDEVFSRREKEHYFAETAVYNNYRYGTPLNELRDDKLIILEPQGLQSFLRLKV
                     CEVVAIFLKTKEECRIERMRNRHDLEENIVKRIEQDRIDFNYEKIDGLDLIIDTTDLT
                     LSNVADLVYYNYNKILANKKFKHEQLSLFETDKS"
     gene            3704..>4424
                     /locus_tag="PLP02_05740"
     CDS             3704..>4424
                     /locus_tag="PLP02_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPF42976.1"
                     /translation="MKKRRMSPLLVIVLSFMGIIVAGMLLFKLPFSVKSGQEMSWVDA
                     LFTSTSAVCVTGLSTIPDLSATLSVFGKVVLAILIQIGGLGIVTIAIYILVLLGIKIG
                     VTERYVIKEALNQHSISGMIKLVRSIILTSLIIEAFGMIINFIALIPEYDFWQAVGLS
                     AFHSISSFNNAGFDLFGDSSLQNFSSNILLNINTMVLIVVGGIGFIVIQDIIKKRNWK
                     QLSMYSKIVIKTTVVLIVAGTL"
BASE COUNT         1074 a         1043 c          951 g         1356 t
ORIGIN      
        1 ccgcccatga ttttacgttt gatcctggct tttttaatga aatccttgga tccgacgagt
       61 aaagtgccga tcggtgaaca tagccccttg gaaaggcaaa cgctgatgga atcggcgtat
      121 ttggcgatct ctttgacatc gcatttcagg aaagtcgccg cattgaagat tctcgctccg
      181 tctaaatgga ctaacaagtt tttcgatttg gccaaatcat aaactttttt catataatcc
      241 agcggcaaaa cgctgccgcc atgagcattt tcaatgcaaa tgagtctggt ttcggtcgtg
      301 gagatagttc tttccttcac ggttttggca agtttctcta gatcccagat gccattatag
      361 ctttccatgg tgaaaagctg gacgcccgag atgatcgaag acgctcccga ttcatgctgg
      421 acaatatggg catcctgatc gacaatcact tcttcgcccc taacacagtg agtaaaaagc
      481 gccaactggt tggaaaatgt tccggaggga acgaagacgg cgtcatcttt cccgagcatt
      541 ctggcggcca tggcctccag ttcgttcatc gtggggtcgt cagagaatac gtcgtccccc
      601 acttccgcgt ttgtcatagc catcagcata tccgatgtcg gctttgttac cgtgtcgctt
      661 cttaaatcga taaatctttt tgtcattatt gtgcgctcct gaagatcaag gctctctgga
      721 aatccttaga cactttgagt atcgtttcca gcacctcgga atagtattct ttgtattctt
      781 cctgttttac ggcattcaaa ttcttctttt taacctggtc ttcgcgggaa gaatcgtaaa
      841 tctgcatgtc tttttccatt tttgccttgg cgatttgatc gactatcgtc atccgctcca
      901 aaaaaagttc ccggatcttt gcgtcaatgt cgtcgatcct atctcgataa cccttcagtg
      961 tctccatgat ctggctcctt gttttttgtc tataaaaatt ataacacaaa aaaagtctgt
     1021 aaatacagac tttccggata tgatgatgaa atgagtatgt catggacgta aatttatcgt
     1081 tatgtcttct atcatatcta tgcttttttg aacatattac agtgcacttt tcgccatttt
     1141 atgcactgta aataagcttt gtggttgctc actgaaattt gacgcgatca gatggctatg
     1201 acaataaaaa gcccgaagtt tcatcccttc aggcttttta agtatttgtt aatggcgagt
     1261 ccggctttgg cgccgtcatc gaccgctttc gctatctgaa ggagaccgcc gatgcaatcc
     1321 cccgcggcga agaggccggg gatgttcgtc atgtaatttt cgtccaccac gatgttgtta
     1381 tgatcggtga acgcgcccat cctgacggca aaatctgtgg ctgaaggggt tcccaccgcc
     1441 acaaaaagag catcaagcgg atattttccg ttttcggtcg ccacgtattc caagtggtcg
     1501 ataccgccga tttccgtcag ttttccactg tcgaccggat aggtgccggt atcgactgtc
     1561 aacttttccc cgtttgtgaa aatcgtgatg tctttagaga tattggcaag attttccaat
     1621 tcatctttca taaaatctcc ggatccgacg agcccgattt ttttgtcacg gtaaaagaac
     1681 ccgtcgcaaa tcacacaata ggaaatgccg tggccgacaa ggccgtcgaa tcccttgact
     1741 ctcaaattga ttttattaac tccggttgcg agcagcaccg ctttggcaat gcaggtggtt
     1801 tcgcaggttc cttcggtcgt tttgacggca aaaccgtcaa aattatcgat tcccaggact
     1861 tctccctgat agatcggtat tccgagggct cttgcctgat ccaaaccttt ttggaaaaga
     1921 tcggcgcccg acaccgggcc cggtacaccg taataattct cgatgtggcc ggttttcccc
     1981 aaggatccca tgcccttcat cacaattccg accgacagtt tcagccgctt taaatagatc
     2041 gcggcggata cccctgcagg tcctccgccg actacgatca catcatacat tttttagagt
     2101 ccgagacgtt tgagcagatc ttccttgcga agcaatccga cgacctgcgc ggctggtttt
     2161 ccttctttga aaaggatgac cgtcggaatg ctgaaaacac ggaattgctg ggcaagatag
     2221 ccttcttcgt cgacatttac cttaccaact ttgacttttc cttccaagtc ttcggataat
     2281 tccttgatga tcggtcccaa catcatgcag gggcggcacc aggctgccca gaaatcgacg
     2341 atgacgggga gttcggattc aaggacttct tttttaaagt tgtcgtttgt gattttaact
     2401 tccattttat ctattcctcc atctgtttta tcaacatgat tttatcagat taatgagctt
     2461 tttcccagcg atatgcttag aaacgctcaa aatctttttc gtcgagtcta aagactgtcc
     2521 attcgtccat cggcttggct tttaggctgt tgtaaaacgc aatcgcttta gtgttccaat
     2581 caagacaggt ccattccatc cggccgcagt tttcttgttt tgcctcctgg atcaagtata
     2641 aaaaaaactg ttttccgtag cccttgtgac gatagggttc tttgatgaat aagtcttcca
     2701 ggtaaagacc tttcttggct ttgaaggtgg agaaattgta aaaataaagg gcaaatccga
     2761 ccggtttttc attttcaaaa ccgattacgg ctttgcatgt tttgtccacg aacaaagcct
     2821 gatacagttt ttcgggagtc gcttccacct gatctgaaag tttttcgtat tcggcgattc
     2881 ccctgataag atccgttact aggttgcatt cgttcattct aacaggacgg ataaagaaat
     2941 tattcgtcat gatttgtcgg tttcgaacag ggatagctgt tcgtgtttga atttcttgtt
     3001 ggcgaggatt ttattgtaat tgtaataaac cagatcggcg acattcgaca atgtcagatc
     3061 agtggtgtcg atgatgagat ctagcccgtc gattttttcg tagttgaaat caattctgtc
     3121 ctgctcgatc cttttaacga tgttttcctc caaatcgtgc cggttacgca ttctctcgat
     3181 ccggcattct tccttggttt ttagaaatat cgcgaccacc tcgcagactt taagccttaa
     3241 aaaagactga aggccctgcg gttccagaat gatcagtttg tcgtctctga gctcgtttaa
     3301 tggcgtacca taacgatagt tattgtaaac cgcggtttcc gcaaagtaat gttctttttc
     3361 cctacgggaa aacacctcgt cggtcataaa atggtaatca aaaccgtcga tctcgtttat
     3421 cctttgtggt ctcgtagtac aagtgacgac ccgagagatc ggataacggt tgatcatgat
     3481 cttggccgat tcggttttcc cggaggcgct tggtcccaaa agaatcaaca tatctgttct
     3541 cctccgaaat gataaatcca ttatatcatg tttttcttgc aaaaaagcct ttatcatttg
     3601 aaaaaccgtg aaagccggtt tgtgattgct taatataaag aaaagtagta taatacaagg
     3661 cgaattttga aggaaacgtc acactaacga ggtgatttca catatgaaaa aaagacgcat
     3721 gtcgccgtta ttggtgatcg ttctcagctt catgggaatc attgtcgccg gcatgttgct
     3781 attcaaattg cctttctcag tcaaatcggg acaggagatg tcctgggtcg atgctttgtt
     3841 cacatcaact tcagcggtct gtgttaccgg cctttccacc atcccggatc tctccgccac
     3901 actttccgtt ttcggaaaag tcgtgctagc gattctgatc cagatcggcg gactcggtat
     3961 tgtgacgatc gccatctaca ttctggttct tcttggcatc aagatcggcg tcaccgaaag
     4021 atacgtaatc aaggaagctt tgaatcagca ttcgatatcc ggaatgatca aacttgtccg
     4081 gtcgatcatc ttgacttctc tcattatcga agccttcgga atgatcatca atttcatcgc
     4141 tctgattccg gaatatgatt tctggcaggc cgttggtctg tcggccttcc attccatatc
     4201 ctctttcaac aatgccggat tcgacctctt cggcgattcc agcttgcaaa atttcagttc
     4261 caatatattg cttaacatca atacgatggt attgatcgtg gttggcggca tcggtttcat
     4321 cgtcatccag gacatcatca aaaaacggaa ttggaaacag ctgagcatgt attcgaagat
     4381 cgtgatcaag accacggttg ttttgattgt ggccggcacc ttga
//
LOCUS       DAOPJL010000054         4345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_22107_length_4345_cov_7.532634, whole genome shotgun sequence.
ACCESSION   DAOPJL010000054 DAOPJL010000000
VERSION     DAOPJL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22107_length_4345_cov_7.532634"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLP02_05745"
     CDS             complement(<1..435)
                     /locus_tag="PLP02_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPF42977.1"
                     /translation="MSAFLDFSELLQSSNLKKTGHRLEILTLLEGTEEPLSADKIHDK
                     LHRKGVHVPYSTIYRTLETLSSNNIISKLRIEKQQRTLFELNRNQHHHHLICLGCEKI
                     IAVEGCPIPEAFTEKIQKEQGFEILGHRLEFFGYCPECQKKLL"
     gene            complement(432..1274)
                     /locus_tag="PLP02_05750"
     CDS             complement(432..1274)
                     /locus_tag="PLP02_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPF42978.1"
                     /translation="MMLSIFEYRFMQYALISAVMASMVCGVIGVIIVEKKLVMMTGGI
                     AHTSYGGVGLGYLLGFEPILGAFMFAVVAALGIGTIKNRNSGASDIVIALFWALGMAL
                     GIFFIGLMPGYPPTITTYLFGNIISVTTFDLILMAILTAIVMAVILIFFQDWKAYLFD
                     PDFTKLSGKKTGLLQNVLLIVIALSVVVLIRVVGIVLVIALLTAPAATARLVSRKLTS
                     RIAWSVLFSLIYSVSGLLISTGIPIASGAVIVFFSIFSYVAVYIVTVLIKKHKMAVKK
                     GANA"
     gene            complement(1274..2026)
                     /locus_tag="PLP02_05755"
     CDS             complement(1274..2026)
                     /locus_tag="PLP02_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF42979.1"
                     /translation="MNEPLLKIDDLSVSYGDIEALRDVFLEINQGEYVGIIGPNGGGK
                     STLMKAILNLVPYAKGNIACKGLPVHKANVRMGYVPQITEMNRLFPISVYEVVLLARL
                     PKKIQPFFKFGEKDRTETFEILKKVGIGHLATRQISDLSGGEFQKMLIARALATNPEI
                     LFLDEPTAMIDAMYQKQIFLLLKKLSTEMTIVMITHHVQPILKQITRLIYLDKNVIAE
                     GDPQAVYSYVYRASSPVHRKAGMRAGESQVNY"
     gene            complement(2023..2877)
                     /locus_tag="PLP02_05760"
     CDS             complement(2023..2877)
                     /locus_tag="PLP02_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPF42980.1"
                     /translation="MKKIIFLFSAFLLAFSLNACQETTTLNKDKPIILVSIVPEADFV
                     RQVAGDLVEVVTVIPPGYSPATYEPTPQTMVTLAEADIYFTIGVAAEDTAILPSLVGP
                     AIVSLEDFVASIYPDRSFDLDDRDPHIWLSIKRVVVMVNTIASVLADFDPDNASAYED
                     NAEAYVGLLNSLDNLLALSFSGITNNKFIVYHPAYGYFADDYGLDMISIEDEGKEATT
                     AWLAEVVSIAEANDIHTVFYQAEIDSSQVTSFAEEIEGVAVELDPLASDYVANMIKMA
                     ALIRGTVN"
     gene            3129..3575
                     /locus_tag="PLP02_05765"
     CDS             3129..3575
                     /locus_tag="PLP02_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPF42981.1"
                     /translation="MNLEAFNAGCNIIGIRKNNIRYGMCCAWAQMVDYDKLTMLLGSQ
                     SVTGKVLEKGDIVGISALSKGQDDAAIRMGDGHSNETDKWQNIPNEEKDTAILVKGAR
                     SKMVCRIREIIRLKGIEADAFLYLDVLSFEQEPKKEFLGAYEVLDK"
     gene            complement(3618..>4345)
                     /locus_tag="PLP02_05770"
     CDS             complement(3618..>4345)
                     /locus_tag="PLP02_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42982.1"
                     /translation="VCLDKGYDMEDEFYNMDPSEKFISVTMGKNHMGVVTSDGRVFLW
                     GDNSYGQIGDATTTVKIVPTDITSRFGFAADETIVELSLGGNHSVLLTSSGRYFVWGN
                     NADGQVGNNATGYITAPNEFSYVFGLPTGVKIVDIDAGFRYSIIIASDGNILATGNNA
                     YGQIGNNSTSTCYVPDSPIMNILFSSESFQYLYDETIAAYIPERESYTLDGWYQDLEM
                     SVPYVCGNMPSSDLILFGIWIPC"
BASE COUNT         1159 a         1000 c         1101 g         1085 t
ORIGIN      
        1 gagaagcttt ttctggcatt ccggacagta tccgaaaaat tccagtctgt gtccgagaat
       61 ctcgaaacct tgttctttct ggattttttc ggtaaaagcc tcgggaatcg ggcagccttc
      121 gacggcgatg attttttcgc atcctaagca tatcagatga tgatggtgct ggttgcggtt
      181 tagttcgaac agcgtccttt gttgcttctc gatccgaagc ttcgagatga tattgttgga
      241 agaaagtgtc tcaagcgtac ggtaaatcgt ggaatagggg acatgtacgc ctttcctgtg
      301 caacttgtca tgaatcttgt cggccgacag cggctcttcg gtgccttcga gcaaggtgag
      361 gatctcgagc ctgtgaccgg tttttttcag attgcttgat tgcagcaatt ccgaaaaatc
      421 aaggaaagcg ctcatgcatt tgcacctttt ttcactgcca ttttatgttt tttgatcagg
      481 acggtaacga tatagaccgc aacataggag aagatggaga agaaaacgat cacggctccc
      541 gaagcaatcg ggatcccggt cgaaatcaga agtccggaaa cgctgtagat aagggaaaac
      601 agcaccgacc aggcgatcct ggatgtcagt tttcgcgaaa caagtctggc cgttgcggcc
      661 ggagccgtca gcaacgctat gacaagcacg atccccacca cccggatcaa aacgacaacg
      721 gaaagcgcga tgacgatcaa aagcacgttt tgcagaagac cggtcttctt tcccgatagt
      781 ttagtgaagt cagggtcgaa cagataggct ttccagtcct gaaagaaaat caggataacc
      841 gccatgacga tagcggtcag gatggccata aggatcaagt cgaaggtcgt tacggaaatg
      901 atgtttccga aaagataagt agtgatcgtg ggcggatagc cgggcatgag accgatgaag
      961 aagataccaa gagccattcc taacgcccag aagagcgcga tcacgatgtc gctcgcgccg
     1021 gaattccggt ttttgatcgt tccgattccc aaagccgcca cgactgcgaa cataaaagcg
     1081 cccagaatcg gttcgaatcc gagcagatat ccgagcccga cgccgccgta cgaagtgtgg
     1141 gcgatgccgc cggtcatcat cacaagtttt ttttcgacga ttatgacgcc gatgactccg
     1201 cacaccatgg aggccataac ggcggaaatc agggcgtatt gcatgaaacg gtattcgaat
     1261 atcgataaca tcatcagtag ttcacctgac tttctccggc ccgcatgccg gccttcctgt
     1321 ggaccggaga agaagcgcgg tacacatagg agtaaactgc ctggggatcg ccttccgcga
     1381 tcacgttttt atcgagataa atgagtcttg taatctgttt cagaatcggc tggacgtgat
     1441 gggtgatcat cacgattgtc atttcggtgc tgagcttttt caaaagcaga aaaatctgtt
     1501 tttggtacat ggcgtcgatc atggccgtcg gctcgtccaa aaacaggatc tccggattcg
     1561 tcgccagcgc tctcgctatc agcattttct ggaattcgcc gccggaaaga tcgctgatct
     1621 gtctcgtggc cagatggccg attccgactt tcttcagaat ctcgaaagtc tcggttcggt
     1681 ccttttcgcc gaatttgaag aacggctgga ttttcttggg cagccttgct agcagaacca
     1741 cttcgtatac ggaaatcggg aacaatctgt tcatttccgt aatctggggg acatatccca
     1801 tccgcacgtt ggctttgtga accgggaggc ctttgcaggc aatgttgcct ttggcatacg
     1861 gaaccagatt caatattgct ttcatcagcg tggattttcc gccgccgttc gggccgatta
     1921 tgccgacata ttcaccttgg ttgatttcaa ggaaaacatc tctcaatgct tcgatatctc
     1981 cgtaagaaac ggacaagtcg tcaattttca acaaaggttc gttcaattca cagtccccct
     2041 tatcaaagcg gccatcttga tcatgttggc cacataatct gaggccagcg ggtcgagttc
     2101 gacggccacg ccttcgattt cttcggcaaa tgacgtcact tgcgaggaat cgatttcggc
     2161 ttggtaaaac acggtatgga tatcattcgc ttcggcgatc gagacgacct ccgccagcca
     2221 cgcggtcgtg gcctctttgc cttcgtcctc tatggagatc atgtcgagtc cgtagtcatc
     2281 cgcaaaatac ccgtatgcgg gatgataaac gatgaacttg ttgtttgtga tgccggaaaa
     2341 agaaagggcc agaagattgt cgagagaatt caacaatccg acataagctt cggcgttatc
     2401 ctcgtatgcc gatgcgttgt cgggatcgaa atccgccaac acggaggcta tcgtgttcac
     2461 catgacgacg acgcgcttga tggaaagcca gatatggggg tcgcgatcgt caagatcgaa
     2521 tgatcggtcg ggatagatgc ttgcgacaaa gtcctctaag gaaacgatcg ccggtccgac
     2581 gagagacggc aatatcgccg tgtcctcggc tgccacgccg atcgtgaaat aaatatcggc
     2641 ttccgccaaa gtgaccattg tctggggcgt cggctcgtaa gtggcgggac tgtatcccgg
     2701 aggaatgacg gtgacgactt cgaccaggtc gccggctacc tgcctgacga aatcggcttc
     2761 cggtacgatt gacaccagaa taatcggttt gtccttattc aatgtggtgg tctcttgaca
     2821 ggcattcagt gaaaatgcca gaagaaacgc agaaaagaga aaaatgattt ttttcattga
     2881 cacgacctct gctttcaaat gcaaatggga ctcattccca tttgatatca ttatatcatt
     2941 ttgacgaaac catgacaaga aagattgtaa aaacacttca tttacttaaa caacgacaga
     3001 acggtatttg ttgtgaaata ttacattctt ttgttatatg gaacaacaga agcacaatta
     3061 aaatgaaatc atgacttgtt actgtaattt tgtgataaaa tcattatgaa agaaaaggag
     3121 atggttttat gaatctggaa gctttcaatg ccggatgcaa catcatcggc atcaggaaaa
     3181 acaatatccg ttacggtatg tgctgcgctt gggcgcagat ggtcgattat gacaaactga
     3241 ccatgcttct cggcagtcag agcgtcaccg gaaaagtatt ggaaaaaggc gatatcgtag
     3301 ggatttccgc cttgtcaaag ggtcaggatg atgccgcgat aagaatgggc gacggacata
     3361 gtaacgagac cgataaatgg caaaatattc cgaatgagga aaaagacaca gccattcttg
     3421 tgaaaggcgc cagatcgaaa atggtttgcc gcatcaggga aataatcagg ctgaagggga
     3481 tcgaagcgga cgcctttttg tatctcgatg tcctgtcttt cgaacaggag cccaaaaaag
     3541 agtttctcgg cgcttacgaa gttttggata aataaaaaag ttctccagat cggagaactt
     3601 ttattttgac aaacaaatca acagggaatc cagattccga acagtatgag atccgatgaa
     3661 ggcatattcc cgcaaacgta aggaacggac atttccagat cctgatacca tccgtctaat
     3721 gtataagatt ccctttcggg gatgtaagcg gctatcgttt cgtcatacaa atactggaac
     3781 gattcggaag aaaacaggat attcattatg ggcgaatccg gaacataaca agtagaagtg
     3841 ctgttgtttc cgatctggcc gtaggcgttg ttgccggtag caaggatatt gccatcggat
     3901 gcaataatga tggaataacg gaaaccggca tcgatatcga caatcttgac tcctgttgga
     3961 agtccgaaca cataactgaa ttcattgggg gccgtgatat atccggtagc attgtttccg
     4021 acctggccgt cggcattgtt tccccagaca aagtatcttc ccgaactggt aagcaatacc
     4081 gaatggttgc ctccgagcga caattcgaca attgtctcgt cggccgcgaa accgaatctc
     4141 gaggtgatat cggtgggaac gattttcacg gtcgttgtcg catcgccgat ctgaccgtat
     4201 gaattgtcgc cccagaggaa gacgcggcca tcggaggtga cgacgcccat atggttttta
     4261 cccatagtga cggaaatgaa tttttctgac ggatccatgt tgtagaattc gtcttccata
     4321 tcgtatccct tgtcgaggca tacgg
//
LOCUS       DAOPJL010000055         4290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_22490_length_4290_cov_6.753955, whole genome shotgun sequence.
ACCESSION   DAOPJL010000055 DAOPJL010000000
VERSION     DAOPJL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22490_length_4290_cov_6.753955"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2534
                     /gene="ppdK"
                     /locus_tag="PLP02_05775"
     CDS             <1..2534
                     /gene="ppdK"
                     /locus_tag="PLP02_05775"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPF42983.1"
                     /translation="KLGMPVPQGFAVSTEACTQYYKDGRMINDEIMAQVENALAETEK
                     VVGKKFGDINNPLLVSVRSGARVSMPGMMDTILNLGLNDQAVEGLANLTNNPRFAYDS
                     YRRFIQMFSDVVMEISKSNFEEILDSRKEAKGVEQDTELDADDLKEIVKNYKEKYLKL
                     KGEPFPQDPKVQLVEAIKAVFRSWDNPRANTYRRLNNIPYEWGTAVNVQQMVFGNMGD
                     DSGTGVAFTRNPSTGEKKFFGEYLFNAQGEDVVAGIRTPKPIFELEKDNPAVYAQFLD
                     ISSKLEAHYKDMQDMEFTIEKGKLYMLQTRNGKRTAQAALKIAISMVDEGLISEKEAV
                     LRVEPKQLDSLLHPQFDQESLKNATVIATGLNASPGAASGMAVFTAERAKELAKKGNP
                     VVLVRQETSPEDIEGMVVAKGVLTARGGMTSHAAVVARGMGTCCVAGAGSIKVYEEAK
                     YFEVGGKKYNEGDFISLDGSTGKIYGEKIKTVDATVSGDFAKLMGWADKYRALKVRTN
                     ADNPRDAKVAYNYGAEGIGLCRTEHMFFDADRIPAMREMIVSKTLEERKAALAKLLPM
                     QKKDFIGLYEEMHGFPVTIRYLDPPLHEFLPTAKEDIEELAKDMGITYEELKATVDSL
                     HETNPMLGHRGVRLTITYPEIAVMQTTAVIEAAIDVNSRGGKVVPEIMIPLVGDVKEL
                     KYVKDIVVKTADEIIAKNNVDLKYHVGTMIEIPRAALLADEIAKEAEFFSFGTNDLTQ
                     MTFGFSRDDAGKFLADYYDKKIFESDPFAHVDTKGVGKLMKMAVELGRSVRPDIKLGI
                     CGEHGGDPASVEFCHSIGLTYVSCSPFRVPLARLAAAHANIKNPR"
     gene            2663..3166
                     /locus_tag="PLP02_05780"
     CDS             2663..3166
                     /locus_tag="PLP02_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42984.1"
                     /translation="MFRLLFCGLKGMDEVSINKLVSKKRAFITSLVLMGIALIIGISM
                     IITSGLKILSQGITGMDDYGFWIVFAFVITGQLFFVVSICIALYGRTKALFPKTKTWV
                     YILLSIAVYYAASIFSLALILITILLKIPVFIGLFILCWLLLGIYIWIFVNLFHIVLR
                     LSQNPLS"
     gene            3442..>4290
                     /locus_tag="PLP02_05785"
     CDS             3442..>4290
                     /locus_tag="PLP02_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPF42985.1"
                     /translation="MSELLQELTMLKGIPANERLVREFIELHAAGKAEISHDKLGSVI
                     LKKTGTADGPRIMVAGHMDEIGFIVSEITKEGYIKFIPAGGWWGHVLLSQQFVLTTME
                     GKEIRGVIGSKPPHILDPEERKKVVELNDMYLDIGVANKEEALEQGIRVGDMITPYIE
                     FKKLANPKYLLAKAFDNRIGIGLVLEVLDRLQKMPHPNTYYGVGSVQEEVGLRGAGTS
                     AFQIDPDIGIALDVTIATDYPGGANDVCLGKGPCLMVYDSSMVGHVGLRRFVTELADK
                     ENIPYQL"
BASE COUNT         1214 a          997 c         1048 g         1031 t
ORIGIN      
        1 ccaaactcgg aatgccggtt ccccaaggat ttgccgtttc aaccgaagct tgcacgcaat
       61 actacaaaga cggacgcatg atcaacgatg aaatcatggc ccaggtcgaa aatgctctcg
      121 ccgaaaccga aaaagttgtc ggaaagaaat tcggagacat caacaaccca ttgctcgtat
      181 ccgtgcgttc gggagccagg gtttccatgc ccggaatgat ggacacgatt ctgaatctcg
      241 gtcttaacga ccaagcggtc gaaggacttg ctaatttgac caacaatccg cgtttcgcct
      301 atgactcata ccggagattc atccagatgt tctccgatgt cgttatggag atttccaaga
      361 gcaacttcga agaaattctc gatagcagga aagaagccaa aggcgtggag caggataccg
      421 agcttgacgc cgacgatctc aaagaaatcg tcaagaatta caaggaaaaa tatctgaagc
      481 tcaagggaga accgttcccg caggatccta aagttcaatt ggtggaagcc atcaaagccg
      541 tattccgttc ctgggacaac ccacgcgcca acacatacag acgtcttaac aatattcctt
      601 acgaatgggg aacagccgtc aacgtccaac agatggtctt cggaaacatg ggagacgatt
      661 ccggaaccgg cgtcgctttc acgagaaatc cgtcgaccgg cgagaagaag ttcttcggcg
      721 aatatctgtt caacgcccag ggcgaggatg tcgtcgccgg aatcaggact ccgaaaccga
      781 ttttcgaact tgagaaggac aaccctgccg tttacgctca attcttggat atttcttcca
      841 aactggaagc tcattacaaa gatatgcagg acatggagtt caccatcgaa aaaggcaaac
      901 tctacatgtt gcagacaaga aacggaaaac ggaccgctca ggccgctttg aagatcgcca
      961 tatcgatggt cgatgaaggc ctcatcagcg aaaaagaagc cgttctcaga gtcgaaccga
     1021 aacaactcga cagcctgctt cacccgcagt tcgatcagga atctctgaaa aacgccaccg
     1081 tcatcgccac tggactcaac gcttctccgg gcgctgcttc cggcatggcc gtcttcacag
     1141 ccgaacgtgc caaggaactc gccaagaagg gaaatcccgt cgtactggtt cgtcaggaaa
     1201 catctccgga agatattgaa ggaatggtcg ttgcgaaagg tgttttgacc gccagaggcg
     1261 gaatgacttc gcacgccgcc gtcgtcgcaa gaggcatggg aacatgctgc gtggccggag
     1321 ccggatccat taaagtctac gaagaagcga aatacttcga agtgggcgga aagaaataca
     1381 acgaaggcga tttcatctcg cttgacggat ccacaggaaa aatctacggt gaaaagatca
     1441 aaacggtcga cgccacagtt tccggcgatt tcgccaaact gatgggttgg gccgacaaat
     1501 accgggccct caaagtaaga accaacgccg ataatcccag agacgccaag gttgcataca
     1561 actacggtgc cgaaggcatc ggactatgcc gcaccgaaca tatgttcttc gatgctgacc
     1621 ggattcccgc tatgcgcgag atgatcgttt cgaagacact agaagaaaga aaagctgctt
     1681 tggccaaact gcttccaatg cagaaaaagg attttatcgg tttatacgaa gaaatgcacg
     1741 gattccccgt caccatccgt taccttgatc cgcctttgca tgaattcctc ccgaccgcca
     1801 aagaagacat tgaagaattg gccaaggata tgggcatcac ctatgaagag ctgaaagcca
     1861 cggttgacag tcttcatgaa accaatccga tgttgggaca ccgcggagtc agactgacga
     1921 tcacctatcc ggaaatcgct gtcatgcaga caacagcggt catcgaagcc gccattgacg
     1981 tgaacagccg cggcggcaaa gttgtaccag aaatcatgat tccgcttgtg ggagacgtaa
     2041 aagaacttaa gtatgtcaag gatatcgttg tcaagacagc cgacgaaatc atcgccaaaa
     2101 acaatgtcga tcttaaatac catgtcggta cgatgatcga aatcccacgt gccgcattgt
     2161 tagccgacga gatcgccaaa gaagccgaat ttttcagctt tggaaccaat gatctaacac
     2221 agatgacttt cggattcagc agagacgacg ccggcaaatt cctggccgat tactatgaca
     2281 agaagatctt cgaaagcgat ccgttcgctc atgtggatac taagggtgtc ggcaaactca
     2341 tgaagatggc cgtcgaactc ggaagatccg tccgtcctga catcaaactg ggaatctgtg
     2401 gcgaacacgg cggcgatcca gcttctgtcg agttctgcca ttcaatcgga ctgacttatg
     2461 tttcctgctc gcctttccgg gtgccgcttg cccgtttagc cgcagcgcat gcgaacatca
     2521 agaatccgcg ctaaatattt aatcatccaa agacgatgca tacaaatgcg tcgtcttttt
     2581 cttttatcaa taatacaaaa ccattgaagc gctgattagt tcatgttaat attgctatat
     2641 caggatcggg gaggaataga tcatgttcag gctgttgttt tgcggtttga aaggaatgga
     2701 tgaagtatct ataaataaac tcgtttccaa gaaaagagct ttcattactt cgctagtgtt
     2761 gatggggatt gccctgatta tagggatttc catgatcata accagtggct tgaagatttt
     2821 gtctcaaggt ataaccggta tggacgacta tggtttctgg atcgttttcg catttgttat
     2881 aaccggtcaa ttgttttttg tcgtttccat atgcatcgcc ttatacggaa ggaccaaagc
     2941 gcttttccca aaaacaaaga cctgggtgta tattttgctt tctatagcgg tttattatgc
     3001 cgcaagtatc ttttccttag ccttgattct tataactatt ttgttgaaaa ttcccgtttt
     3061 tatcggtctc tttattcttt gctggttgct ccttggcatt tatatatgga tatttgtcaa
     3121 tctgtttcat attgttttgc gcctgtctca aaacccgctt agctgaagtg caaacggttt
     3181 tcactgactt tttggagggc ttgttgacaa agcaacaagc gttatatata atgaatttgt
     3241 acatggacgg ttggaaatca ctcacccgcg acctacaatg aaagaaaagg gagtcgaggc
     3301 gtttccggtg ttgaatgcct aaatctaatt ttttaggaat cctgaaggaa aagcaagaga
     3361 cgcccaccat tgggtagatg tttgtctgct cgagtgattt ccaactgtcc atgtattttt
     3421 atttgctgaa ggaggtaaaa tatgagtgag cttttacaag aattaacaat gttgaaaggc
     3481 attcctgcca atgaaagact ggtcagggaa ttcatcgagt tgcacgctgc cggcaaggcg
     3541 gaaataagtc atgataaatt gggttcggtc attcttaaaa aaaccggaac agcagatggc
     3601 ccaagaatca tggttgcggg acacatggat gaaattggct tcatcgtttc ggaaataacc
     3661 aaagagggtt atatcaaatt cataccggcc ggaggctggt ggggacatgt gctcttgagc
     3721 cagcaattcg ttctgaccac gatggaaggc aaagagatca gaggagtcat cgggtcgaaa
     3781 ccgccacaca tccttgatcc cgaagagaga aagaaagtcg tcgaacttaa tgacatgtat
     3841 ttagacatcg gcgtcgcaaa caaggaagaa gctttggaac aaggaatcag agttggcgac
     3901 atgattacgc catatatcga attcaagaaa ctggccaatc ccaaatatct gctggctaaa
     3961 gctttcgaca accggatcgg catcggcctg gttttagaag ttctcgaccg cttgcagaag
     4021 atgccgcatc cgaacacata ttatggcgtt ggttcggttc aggaagaagt cggacttcgg
     4081 ggagcgggaa cttccgcctt ccagatcgat ccggatatcg gcattgccct ggatgtgacg
     4141 atcgcaaccg attatccggg aggcgccaac gatgtttgtc tgggaaaggg accctgtctc
     4201 atggtatatg acagttccat ggtcggccat gtgggcttga gaaggtttgt gaccgaactg
     4261 gccgacaagg aaaatattcc ttatcagctg
//
LOCUS       DAOPJL010000056         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_23235_length_4195_cov_5.976812, whole genome shotgun sequence.
ACCESSION   DAOPJL010000056 DAOPJL010000000
VERSION     DAOPJL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23235_length_4195_cov_5.976812"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLP02_05790"
     CDS             complement(<1..758)
                     /locus_tag="PLP02_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPF42986.1"
                     /translation="MSNYDRLVFELSVPGRRGYTLPNQFASKYKITELKGLSRKDSPN
                     LPELSELDVVRHYTNISQKNFAVETGFYPLGSCTMKYNPKINEEIAGSQSLGLLHPLQ
                     PASTIQGALEINYKLRGALSEISGMSDFTLNPYAGAHGELTGLMIMKKYHDDRKDFKR
                     TKVIVPDSAHGTNPASTAVCGMEVVEIASDENGRVDLESLKANLSDEIAGMMLTNPNT
                     LGVFENDICEIAKLVHLAGGLMYYDGANLNAILG"
     gene            complement(755..2089)
                     /gene="gcvPA"
                     /locus_tag="PLP02_05795"
     CDS             complement(755..2089)
                     /gene="gcvPA"
                     /locus_tag="PLP02_05795"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPF42987.1"
                     /translation="MFKYFPHTDEDIEAMKRAIGIASVDELFSDVPKDVFFRRKYNLP
                     DAMSEIELRAYFDKLGKANSDLVCFAGLGTYDHYDPAVIKSLTSRQEFLTAYTPYQPE
                     VSQGTLQYIFEFQSMIQILTGMDAANASMYDGATATAEACFMAEAIARRKLILVSKTV
                     NPYVTDVLKTYCRPRGISIEIIPETDGITDIAELEKMIDGNVAAVVAQKPNFFGLIED
                     FGRISEIIHANGSVFIENADLSALGILKTPKEDGADIVVGDCQTLGIPMSYGGPGAGY
                     MATIKAHIRKLPGRIVGLSKDVDGKRAYVLTLQAREQHIRREKANSNICSNQSLMALY
                     VTIYLSLMGPEGLREVNELSYQGAHYLYDRLIATGKFRPAFKGPFLKEFCLKTNIPAS
                     TIQEVLLKNGIFGAIPMGEFGEEYQNLVSFAVTEKRTKEEIEHLVSLLEVVA"
     gene            complement(2099..2479)
                     /gene="gcvH"
                     /locus_tag="PLP02_05800"
     CDS             complement(2099..2479)
                     /gene="gcvH"
                     /locus_tag="PLP02_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPF42988.1"
                     /translation="MSKAADGYLYTESHEWVKPLDDKTVLVGITDYAQAQLGTVVFVD
                     LPEAGKQLKMHEELGAVESVKAASDIYSPVSGKVLEVNEDLVGEPEKINNDPYEAWLV
                     KVELDDKSELQKLLSKADYEKISK"
     gene            complement(2492..3583)
                     /gene="gcvT"
                     /locus_tag="PLP02_05805"
     CDS             complement(2492..3583)
                     /gene="gcvT"
                     /locus_tag="PLP02_05805"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPF42989.1"
                     /translation="MAESKKTTLYDYHVAKGAKMEDFAGFMMPIVYTSIPEEHKAVRE
                     KAGMFDVSHMGEVRVKGKDALAYVDHVFANEITSKDNGKVVYGMMLYENGTIVDDLLV
                     YKVSDTECFLVVNASNTGKDYAWLVANTGNYDVTVENVSDEYSQIALQGPEAEEYMKK
                     IFGLDLSDLAFYTFGNFSILGHEVLLSRTGYTGEDGFEIYGDHIAVLEIWNALDHAGI
                     VPCGLGARDTLRFEASLPLYGHEISDKITPLEAGLKMFVALDKTDFIGKKALDEQMEK
                     GLTRRVVGLELKEMGIPRQGYPVYQDAEEIGYITTGYLSITLNKPIAMVMLKTEYAKK
                     GTEVSVEIRKRMVPAFVRDKKFLQKNYKK"
     gene            complement(3602..>4195)
                     /locus_tag="PLP02_05810"
     CDS             complement(3602..>4195)
                     /locus_tag="PLP02_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPF42990.1"
                     /translation="VSGNAFYRVNNRSVVHGTMLYDTDLEAMVKAITPNNEKLVSKGI
                     DSVRKRVTNLREHFNMPIEDFKRYMHDHLCDQDIVLTDRDVEEIRKIEEGYLELDFIY
                     GKNPSYSVTRKGRVQAGYLEISLELKNNKVKKINMLGDYFLISDPDVFLHRLVGIDFT
                     KAAFETALKEINLAEYVYNLDTTAFLDIMFKAEANKL"
BASE COUNT          986 a         1012 c          960 g         1237 t
ORIGIN      
        1 ttaccaagaa tggcgttcaa attggccccg tcatagtaca tgagaccgcc ggctaaatgt
       61 acgagtttcg ctatttcgca aatatcgttt tcaaatacgc ctaatgtatt gggattcgtc
      121 agcatcattc cggcgatctc gtcggaaaga ttggctttaa gactttcgag atcgacgcgt
      181 ccgttttcat cagaagcgat ctcaaccact tccatgccgc atacggcagt ggaagccggg
      241 ttggttccgt gcgcggaatc gggaacgatg acttttgtac gtttgaaatc ttttctgtca
      301 tcatgatatt ttttcataat catgagacca gtcaattctc cgtgggctcc ggcgtatgga
      361 ttcaaggtga agtcggacat accggatatt tcggacagtg ccccgcgaag cttgtagttg
      421 atttccaaag cgccctgaat cgtggaagcc ggttgcaaag gatgcaacaa gcctaacgat
      481 tgcgatccgg ctatctcctc gtttattttc ggattgtact tcatcgtgca ggaaccgaga
      541 ggatagaatc cggtttcgac cgcaaagttc ttttgggata tgttggtgta atgtctgaca
      601 acatccagtt ccgataattc cggaagattt ggactatctt ttctcgacaa tcctttaagt
      661 tccgttattt tatatttgga agcgaattga tttggcaggg tatatccacg ccgtccggga
      721 acggacagtt cgaatacgag acggtcataa ttactcatgc gaccacctcc agaagagaaa
      781 ccaaatgttc gatttcttct tttgtccttt tttcagtaac cgcgaacgac accaggtttt
      841 ggtactcctc gccgaattct cccatgggaa tcgctccgaa aataccgttt ttcaacaata
      901 cttcctgaat ggttgacgcc ggtatgttcg tctttagaca gaattccttg aggaaaggtc
      961 ctttgaaggc tggccggaat ttaccggtcg cgatcagacg atcatacagg taatgggcgc
     1021 cctgatagga aagctcgttt acttccctta atccttccgg tcccatcagg gacaaataaa
     1081 tggtcacata cagggccatc aaggactggt tggaacagat attgctgttg gccttctcac
     1141 gacggatatg ctgttcacgc gcttgtagtg tcagaacata ggcgcgtttt ccgtcgacat
     1201 ccttgcttag gccgacgatt cggcctggca atttacggat atgagctttg atcgttgcca
     1261 tgtaaccggc gccggggccg ccgtaagaca tcggaattcc aagtgtttgg cagtcgccga
     1321 cgacaatgtc ggcaccgtct tcttttggtg ttttcaaaat tcccaatgcc gataaatcgg
     1381 cattttcaat gaatacggat ccgttggcgt ggattatctc cgatatccgt ccaaaatctt
     1441 cgatgaggcc aaagaaattc ggcttttgag cgacgactgc tgccacattt ccgtcaatca
     1501 tcttttccag ttccgcgata tccgtgatgc cgtctgtttc cggtatgatc tcgatagaga
     1561 tgccgcgcgg acggcaatag gttttcaaaa catccgtaac ataaggattt acagtcttgg
     1621 aaacaaggat cagtttcctt ctggcgatag cttcagccat gaaacaggct tccgccgtgg
     1681 ctgtggcgcc atcatacatt gaggcgtttg ccgcatccat ccctgtcaga atctgaatca
     1741 tcgactggaa ttcgaatatg tattggagcg tgccctggct cacttccggt tggtagggag
     1801 tgtaggcggt caaaaattcc tgccggctcg taagactttt tatgacggcg ggatcatagt
     1861 ggtcataggt gccaaggccg gcaaaacaaa ccagatcgga attggctttt ccaagtttat
     1921 cgaaataagc cctaagttcg atttccgaca tggcgtccgg caaattatat ttacgacgaa
     1981 agaagacatc tttggggacg tctgaaaaaa gttcatccac ggaagcgatg ccgatggcac
     2041 gtttcatcgc ttcgatatct tcatctgtgt gtggaaagta tttgaacatt gctgtacctt
     2101 atttgcttat cttttcgtaa tctgccttgg aaagaagttt ttgcaattcg gacttgtcgt
     2161 caagctcgac ttttaccaac caagcttcat aaggatcatt gttgatcttt tctggttcgc
     2221 cgaccaaatc ttcattgact tcaagaactt ttccggaaac gggactatag atgtctgaag
     2281 ccgctttgac agattcgaca gctcctagtt cctcgtgcat tttgagttgt tttcccgctt
     2341 cgggaagatc aacgaacacg actgtcccca attgcgcttg ggcgtagtcg gtgataccga
     2401 ccaaaaccgt cttgtcgtcc aaaggtttaa cccattcatg actctcggta taaagataac
     2461 cgtctgctgc tttactcatt tacaacacct ctcatttttt gtagtttttc tgcaagaatt
     2521 ttttatctct tacaaaggcc ggcaccatcc gtttccggat ttctacggaa acttcggtcc
     2581 ctttctttgc atattccgtt tttaacatga ccatggcgat cggtttgtta agagtgatcg
     2641 acagataacc agtcgtaata tagccgattt cttcggcatc ttgataaacc ggatagcctt
     2701 gtcgggggat tcccatttcc ttcagttcca gtccgacgac ccggcgggtc aatccttttt
     2761 ccatttgctc gtcaagagct ttctttccga tgaaatcggt tttgtccaag gcgacaaaca
     2821 tcttgagtcc tgcttcaagc ggcgtgatct tgtcgctgat ttcatgaccg tataacggca
     2881 agctggcttc aaatcggagc gtatcgcggg caccgaggcc gcacgggacg attccggcat
     2941 gatcaagagc attccatatc tccaaaaccg ctatgtgatc tccgtaaatc tcgaatccgt
     3001 cctcgccggt ataaccggtg cgcgataata aaacttcatg tccgagaatc gagaaatttc
     3061 caaaagtata aaaagcgaga tcggaaagat caagtccgaa tatcttcttc atgtattctt
     3121 ccgcttccgg tccctgcaaa gcgatctggg aatattcgtc ggatacattt tcgacggtaa
     3181 cgtcgtagtt tccggtattg gccaccagcc aagcgtaatc cttgccggtg tttgatgcat
     3241 tcaccaccag gaaacattcg gtatcggaaa ctttgtagac gagaaggtcg tcgacaatgg
     3301 ttccgttttc gtaaagcatc atgccgtaca cgactttgcc gttatccttt gatgtgatct
     3361 cattggcgaa cacgtgatcg acataggcga gggcgtcctt gccttttact ctgacttctc
     3421 ccatatggga aacgtcgaac attcccgcct tctccctgac cgccttgtgt tcttccggta
     3481 tggaagtgta cacgataggc atcatgaatc cggcgaaatc ttccattttg gcgccttttg
     3541 cgacatgata gtcgtatagc gtcgttttct tgctttctgc catatttgcc tccgggtttt
     3601 attataactt atttgcttcc gctttgaaca taatgtccag aaaagcggtc gtatccaggt
     3661 tgtagacata ttccgccagg ttgatttcct tcaaagcggt ttcgaatgcc gctttggtga
     3721 aatcgattcc taccagacga tgaaggaata catcggggtc gctgatcagg aaatagtcgc
     3781 caagcatatt gatttttttg accttgttat tcttaagctc caggctaatt tccagatagc
     3841 cggcctgaac gcgtcctttt ctcgtcacgg aataactggg atttttcccg tagatgaaat
     3901 caagttccag atatccttct tcgatttttc tgatttcttc gacgtcccgg tccgtgagaa
     3961 caatgtcctg atcgcacaaa tgatcgtgca tgtatcgttt gaagtcttca atcggcatgt
     4021 tgaaatgctc ccggagattc gtcacccgtt tgcgaaccga atcaatgcct tttgacacca
     4081 gtttttcgtt gtttggcgtt atggctttga ccattgcttc gagatcggtg tcataaagca
     4141 tcgtcccatg gacgacgctt ctgttgttca cccggtagaa cgcgtttcca ctgac
//
LOCUS       DAOPJL010000057         4054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_24377_length_4054_cov_5.974494, whole genome shotgun sequence.
ACCESSION   DAOPJL010000057 DAOPJL010000000
VERSION     DAOPJL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24377_length_4054_cov_5.974494"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLP02_05815"
     CDS             complement(<1..904)
                     /locus_tag="PLP02_05815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242249.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPF42991.1"
                     /translation="MNANKDKLVKAITGRDEDFAQWYTDVCKKAELMDYTETKGFIIY
                     RPYGYKLWEGIQAYLDNELKKTGHENVYFPLVIPESLFQKEKEHVEGFAPETAIVTIG
                     GNEELTDRLIIRPTSEVLFCDHYAKIVSSYRDLPKKYNQWCSVVRWEKTTRPFLRGSE
                     FLWQEGHTIHETEAEAMKETLDILSIYEKLGKELLAIPFVTGIKTEKEKFAGAEATYT
                     IEALMHDNKALQSGTTHYFGQGFAKAFNIQFQDREKNLQYVYQTSWGVTTRLIGAIIM
                     VHGDDSGLVLPPRLAPIQAVIVPIQ"
     gene            complement(948..2426)
                     /locus_tag="PLP02_05820"
     CDS             complement(948..2426)
                     /locus_tag="PLP02_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HPF42992.1"
                     /translation="MNTLINQLAKYALTYGLLAKDDYEYAINGMLYLLKLDTFVKEDI
                     REDVDYFIVMETLLDQAVAKGLIEDTISSRDNFEAKLVDCLIPRPAEVNRIFREKYHI
                     GPRTATDWFYRFSCDTNYIKTRRIAKNVSYEYAGKYGKLQITVNLSKPENDPKDIVVK
                     AQNTDDEKYPKCALCVENVGFYGTDELAPRSNHRTISMTINNEKDMWAFQYSPYAYFN
                     EHCIVLRKDHFPMKVDRDTFAELIDFINKFPNYLIGSNAGLPIVGGSILSHYHFQGGR
                     YDFPIEKARILRTFKSGRIRAEVLDWPLSVIRLVCNNENRLEDMANQIFEAWKQYTDG
                     DLGILAQTEDGCHNTVTPIIKLDGAEYNLYLVLRNNRRTEKRPYGVFHTREEYFHIKK
                     ENIGLIEVMGLAILPGRLLSELALAKACIRENREFSDYPELEKHLSWLESIRGEATAT
                     DNLDRFFEKEVGRIFESAIEDCGVFKAQDTEAFFRFVEKAIY"
     gene            complement(2423..3418)
                     /gene="galE"
                     /locus_tag="PLP02_05825"
     CDS             complement(2423..3418)
                     /gene="galE"
                     /locus_tag="PLP02_05825"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPF42993.1"
                     /translation="MKIVVTGGAGYIGSHFVKAALNANHEVHVVDNLTTGHREAVDSR
                     AIFHLGDIRDLDFMETVFAGNQFDSCVHFAACSLVPESMEKPFKYFDNNVNGMLCLLE
                     TMKRHQTKTIVFSSSAAVYGDKLEMPITEDDIPMPTNPYGESKLIMEKMMKWADAAYG
                     IRYVSLRYFNVAGASCDQSIGEDHRPETHLIPIVLQVPLGLRENIVIYGNDYDTEDGT
                     CVRDYIHVGDLVSAHLKGLDYLASGKPSDIFNLGNRKGFSNLEIVETASRVTGYEIEH
                     VFGPRRAGDPARLIASNLKAEKILGWIPKKGLEEIIRSAWEFHQKHPYGYEGDDR"
     gene            complement(3415..>4054)
                     /locus_tag="PLP02_05830"
     CDS             complement(3415..>4054)
                     /locus_tag="PLP02_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF42994.1"
                     /translation="DYRHAMFPASLDVYVRYTVKPGILEIDYDVLARENSLVNMTNHV
                     YFNLEGDPSHDLSKTHILEIPASHVVLTDGQMVGNDIIKVAKTGFDFRRPRLLADALN
                     DLVLTNAGNLGIDNYYILSKTRLFDAKIHSVKNNKTLTIKSDYPGLNLYTGNYPDMSI
                     LSNGKPMAFHGALAIEPHFQTNAVNDTRFEGYALMEGQRYHHRISYRLEEQT"
BASE COUNT          987 a          950 c          923 g         1194 t
ORIGIN      
        1 gctggatcgg cacgatgaca gcctggatcg gagccagacg cggaggcaga accaaaccgg
       61 aatcatcgcc gtgaaccatg atgatggcgc cgatcaacct ggttgtgacg ccccatgatg
      121 tctgataaac atattgcagg ttcttctcgc ggtcctggaa ctggatattg aaggctttgg
      181 cgaaaccctg gccgaaataa tgggtggttc cgctctggag ggctttgttg tcgtgcatca
      241 aggcttcgat cgtgtaagtc gcttcggctc cggcaaattt ttctttctca gtcttgatac
      301 cggtaacgaa tggaatcgcc aacaattcct tgccgagttt ttcatagata gataaaatat
      361 cgagcgtttc tttcatggct tccgcttcgg tctcgtggat cgtatggcct tcttgccata
      421 aaaactccga accgcggagg aatggccgtg tcgttttttc ccatctgacg acgctgcacc
      481 attggttgta cttcttcggc aggtcgcggt acgaggagac gattttggca taatggtcgc
      541 agaacaggac ttccgaggtc ggtctgatga tcaaacggtc tgtaagttcc tcatttccac
      601 cgatagtgac gatggccgtt tcgggagcga aaccttcgac atgctctttt tccttttgaa
      661 acaggctttc cggaataacc aaaggaaaat agacattttc atgtccggtt ttcttcagtt
      721 cgttgtccaa ataggcctgg atgccttccc atagtttgta tccgtacgga cggtagatga
      781 taaagccctt ggtttcggta taatccatca gttccgcttt cttgcagaca tcggtatacc
      841 attgggcgaa gtcctcgtcc cggccggtta tggctttgac aagtttgtct ttgttggcgt
      901 tcattgtctc acctcgtgtc gataatatat tatatcactt ttctttgtta gtatatggct
      961 ttttcgacga aacggaaaaa cgcttcggtg tcttgagcct tgaatacacc gcaatcttcg
     1021 atcgcgcttt cgaaaatccg gccgacttct ttttcgaaga aacgatcaag gttgtctgta
     1081 gcggttgctt cacctcggat actttccagc cacgaaagat gtttttccag ttcggggtaa
     1141 tctgagaatt ccctgttttc acggatgcat gccttggcta gggccagttc gcttaaaagc
     1201 cggcccggaa ggatggcgag acccatgact tcgatcaaac cgatattctc ttttttgata
     1261 tggaaatatt cttcccttgt atgaaaaact ccgtaaggac gcttttcggt tcgccgattg
     1321 ttccgaagta cgagatagag attgtactcg gcgccatcca atttaattat cggagtcact
     1381 gtgttgtggc agccatcttc tgtttgagcc aagataccca aatccccgtc agtatattgt
     1441 ttccaggctt cgaagatctg gttggccatg tcttccagac gattttcgtt gttacagacg
     1501 agcctgatga cggaaagcgg ccaatcaagc acttcggctc tgattcttcc ggatttgaat
     1561 gtcctcagga ttctggcttt ctcgatggga aagtcataac ggcctccctg aaaatggtaa
     1621 tgggaaagaa tcgatccgcc gacaatcggt agaccggcat tggaaccgat cagataatta
     1681 ggaaatttat tgatgaaatc gatcagttcc gcaaaggtat ctctatccac tttcatcgga
     1741 aaatgatctt tgcgaagaac tatgcaatgt tcattgaaat aggcatacgg ggaatattgg
     1801 aacgcccaca tgtctttttc gttgttgata gtcatggaaa tagtccggtg attgcttctc
     1861 ggagccaact catccgttcc gtaaaagccg acattttcca cgcaaagcgc gcatttcgga
     1921 tacttttcat catcggtgtt ttgcgctttg acgacaatat ctttcggatc gttttccggt
     1981 ttcgaaagat taaccgttat ctgcaatttg ccgtattttc ccgcatattc gtaagatacg
     2041 ttcttggcga ttcttctggt cttgatataa ttggtgtcac aggaaaacct gtaaaaccaa
     2101 tcggtagccg tacggggacc aatatgatat ttttcccgaa atatacggtt tacttcagcc
     2161 ggtcgcggta tcagacaatc aacgagcttg gcttcaaaat tgtcgcgcga cgaaatagtg
     2221 tcttcgatca gacctttcgc gacagcctgg tccagcaatg tttccatcac gataaagtaa
     2281 tcgacatctt ctctgatgtc ttctttaacg aaagtgtcca gtttaagaag ataaagcatc
     2341 ccgttgatcg catattcata atcatccttg gccaaaagcc catatgtcaa agcgtattta
     2401 gccaactggt ttatcagtgt gttcacctgt catcaccttc gtatccgtac ggatgttttt
     2461 gatgaaattc ccaagcggaa cggatgattt cctccaaacc ctttttcgga atccaaccca
     2521 atattttttc tgcttttagg ttcgatgcta tcagtctggc gggatcgcca gcgcgcctgg
     2581 gaccgaacac gtgttcgatt tcatatccag tcacccttga cgccgtctcg acaatttcca
     2641 gattggaaaa tcctttgcgg tttcccagat tgaatatatc ggatggcttt ccgcttgcca
     2701 gataatcaag tcctttgaga tgggcgctga ccaaatctcc gacatggatg taatcacgga
     2761 cacaggttcc gtcctcagtg tcataatcgt tgccatagat aacgatattt tctcttaaac
     2821 cgagtggtac ttgcaaaacg atcggtatca gatgggtttc cggccggtgg tcttctccga
     2881 tcgattgatc gcaggatgcg ccggcaacat tgaagtaacg caagctgaca tagcggatgc
     2941 catacgcggc atcggcccat ttcatcattt tttccataat cagttttgat tcgccataag
     3001 gattggtagg catcgggatg tcatcttccg ttatcggcat ttccaattta tctccgtaaa
     3061 cagctgcgga cgaggaaaaa acgatcgttt tcgtctgatg tcttttcatg gtttccaaaa
     3121 gacagagcat cccgttgaca ttgttgtcaa aatatttgaa tggcttttcc atggattccg
     3181 gaaccagaga acaggcggca aaatggacgc aagaatcaaa ttgattaccg gcaaataccg
     3241 tttccatgaa atcgagatcg cggatgtctc ccaagtgaaa aatcgctctt gaatcaacgg
     3301 cctctctgtg cccggttgtc aaattgtcca ccacatggac ttcatgattc gcgttcaatg
     3361 cggctttgac gaaatggctg ccgatataac cggcgccgcc ggtaactact attttcatgt
     3421 ttgttcctcc aatctgtaag aaattctatg atgatacctt tggccttcca tcagggcata
     3481 tccttcaaac cttgtgtcat tcacggcatt ggtctggaag tgcggttcga tggcaagagc
     3541 gccgtgaaaa gccatgggct tcccgtttga taaaatcgac atgtctggat aattgccggt
     3601 atagagattt aggcccgggt aatcactttt tatcgtgagc gttttgttgt ttttgacaga
     3661 atgtattttg gcgtcaaaca atctggtttt ggatagtatg tagtagttgt cgattcccag
     3721 attgccagcg tttgtcaaga ccaaatcgtt taacgcgtcc gccaacaaac gcggacgtct
     3781 aaaatcgaag ccggttttgg ctactttaat gatatcattg ccaaccattt gcccgtctgt
     3841 caacaccaca tgcgatgccg gtatttccaa aatatgtgtc tttgacaaat catgggatgg
     3901 atcgccttcc agattgaaat aaacgtgatt cgtcatattg acaagcgaat tctccctcgc
     3961 cagcacatcg tagtcgattt ccaggattcc cggtttaacc gtatatctaa cataaacatc
     4021 caaactggcc ggaaacatgg catgccggta atct
//
LOCUS       DAOPJL010000058         4053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_24388_length_4053_cov_7.608304, whole genome shotgun sequence.
ACCESSION   DAOPJL010000058 DAOPJL010000000
VERSION     DAOPJL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24388_length_4053_cov_7.608304"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1290
                     /locus_tag="PLP02_05835"
     CDS             1..1290
                     /locus_tag="PLP02_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF42995.1"
                     /translation="MERTRKSLRNLIVYQIYVRNFSEAGTFKAVIEDLDRIMELGVNM
                     VYLLPIHPIGKKNRKGSLGSPYSISDYRLINPELGATDDFRKLIRAVHERDMLIMMDV
                     VFNHTSCDSRLLMEHPEWFYHDKAGNFANKAGDWSDVYDLDFSSDKKMWDELAETLAV
                     YADMGVDGFRCDVASLVPLEFWRHARRAVGRESRGVIWLSESVHGSFCKYVRDLGFNC
                     SSESEIYQVFDMAYDYDVRPYMEDYFAGKRPLKDYLEALQRQDEIYPENYVKLKNLDN
                     HDVVRIASYCKNDKDKIENWTGFLFFQKGATMLYAGEEFSCDKTPNLFEKDPIVRKRD
                     ISPYIQKLVAIKKRPVFATGAYKVYIPEVDGVACTTFENAKEKVVGIFNLAKAEGRIS
                     VPLEGGKYRNYLTGELVSVRAGKMNLQNGPIVLQIKK"
     gene            1381..2682
                     /gene="trmFO"
                     /locus_tag="PLP02_05840"
     CDS             1381..2682
                     /gene="trmFO"
                     /locus_tag="PLP02_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPF42996.1"
                     /translation="MGEKSITVIGAGLAGVEAALQIANRNVVVDLYEMKKIRKSPAHK
                     SDDFAELVCSNSLRSDSLENAVGLMKAEMRLLKSEVIEAADKCRVPAGSALAVDRNMF
                     SSYLTEKVRKNPLITIHEEEMKKIPEGIVIIATGPLTDGELASSITKITGQEALYFYD
                     AVAPIIEFDTIDMNVAYYKNRYDKGDGMYINCPMDKDEYDAFYNALIDAKCAPIHDFE
                     MKVFEGCMPVEEMARRGYETLLYGPMKPVGLERDEKRPYAVVQLRQDDLAGTLYNIVG
                     FQTHLLFSEQKKLIRMIPGLKDAKIVRYGVTHRNSYVKAPEVLLSTYQMKNNNNVFIA
                     GQLSGVEGYVESIASGLVAGINAYRLFSRFEPLRFPLETAIGSQANYLEFASPSQFQP
                     MNANFGLFPALDMKYKKRDKKGLLAKRSMETMEEFIKAVKP"
     gene            2679..3587
                     /locus_tag="PLP02_05845"
     CDS             2679..3587
                     /locus_tag="PLP02_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPF42997.1"
                     /translation="MTDKEIIASYKDYLSEQRNYSPHTVTAYMEDVECLENFLMSEDL
                     GNLMTVTDRIARFYVSSLHGHYDPRSIRRKISSVRSLYKLLVDRGELDRNPFQNVELP
                     KESKPLPKFVYEKEMNNFLERIDSSSPIGKRDQAIFEILYGSGLRVSELVTLKIRDID
                     FFNHTLLVHGKGSKDRYVPFHETEKERIKMYLETVRPLFRSRTEEADEGYLFVNFHGG
                     HLTDRGVRKILDTELAKQASTMKLTPHAFRHSFATHLLDNGVDLRTVQELLGHVSLST
                     TQIYTKVSKEKLKEVYMASHPRAKIK"
     gene            3587..>4053
                     /gene="rlmH"
                     /locus_tag="PLP02_05850"
     CDS             3587..>4053
                     /gene="rlmH"
                     /locus_tag="PLP02_05850"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPF42998.1"
                     /translation="MGITIIAVGSLKEDYLKAGVKEYIKRISAYAKLNMIEISDKSLN
                     KNPSQGDIQKVLDQEGLEILKKIKSSTFVVTLEIDGDMMTSDQFAKFIEHTTTYNSSE
                     IVFVIGGSWGISREVKQRSNKSLSLSSMTFPHQLTRMIFLEQLYRAFTIINNEP"
BASE COUNT         1244 a          820 c          949 g         1040 t
ORIGIN      
        1 atggaaagaa caagaaagtc tttgagaaac ctgatcgtct atcagattta cgtgcgtaat
       61 ttttctgaag cgggaacttt taaagcggtg atcgaggatc tagacaggat catggaactt
      121 ggtgtcaata tggtgtatct tttaccgatc catccgatcg gcaagaagaa ccgcaaggga
      181 tcgctcggat cgccttattc gattagcgat taccggttaa tcaatccgga attgggggcg
      241 accgatgatt ttcgcaaact gatcagagcc gttcatgaaa gagacatgct catcatgatg
      301 gatgtcgtat tcaatcatac atcatgcgat tccaggttgc ttatggaaca tccggaatgg
      361 ttttatcatg acaaggctgg taattttgct aataaggcgg gggattggtc cgatgtctat
      421 gatttggact tttcatctga taagaaaatg tgggatgaac tagctgagac tttggcggta
      481 tatgctgaca tgggagttga cggatttcgt tgtgatgttg ccagtttggt accgctggaa
      541 ttctggcgac atgccagaag ggcggtcggc cgcgaaagtc gcggagtcat ctggttgagc
      601 gaatccgtgc atggttcatt ttgcaaatat gttcgtgatc tcggattcaa ctgctcctcc
      661 gaatcggaga tataccaggt cttcgacatg gcatacgatt atgatgtcag accttacatg
      721 gaagattatt ttgccggtaa gagaccgctt aaagattatc ttgaagcttt gcaaagacag
      781 gatgagattt atcctgagaa ttatgtgaaa ctgaaaaatc ttgataatca cgatgtggtg
      841 aggatagcat cctattgcaa gaacgacaaa gacaagatcg aaaactggac cggattttta
      901 ttcttccaaa aaggggccac gatgctttat gcgggcgagg aattctcttg tgacaagaca
      961 ccgaatcttt tcgagaagga tccgattgtc cgtaaaagag atatcagccc ttatatccag
     1021 aaactcgtcg ccatcaagaa aagaccggtg ttcgcaaccg gagcatataa agtctatata
     1081 cctgaagtgg acggtgtcgc ttgcaccaca tttgaaaatg ccaaggaaaa agtcgtcggc
     1141 attttcaacc tggcgaaagc ggagggaaga atcagcgtac ccttggaagg cggcaaatac
     1201 cgaaattatc tcacagggga actcgtatcg gtgagagctg ggaagatgaa tctccaaaat
     1261 ggtccaatcg ttttgcaaat aaagaaatga taactttaac agaaacccac gctaattcgg
     1321 gtttctgttt ttttgtccgg tccgcttatg gtataatcat agtcaaggga cgtgaagagc
     1381 atgggtgaaa aatctattac ggtgattggt gcgggcttgg ctggcgtcga agccgctttg
     1441 caaatagcca accgcaatgt cgtggtcgac ttatatgaaa tgaaaaagat ccgcaaaagc
     1501 ccagcccata aatcggatga ctttgccgaa ctagtctgtt cgaattcctt acgcagcgat
     1561 tccttggaaa acgccgtcgg actcatgaaa gcagaaatgc gtttacttaa aagtgaagtg
     1621 atagaagccg ccgacaaatg ccgggtaccg gctggaagcg cattggccgt agaccgaaac
     1681 atgttctcta gttatctgac agaaaaggtc aggaaaaatc ctttgattac tattcacgaa
     1741 gaggaaatga agaagattcc ggaagggatc gtcatcattg cgaccggccc cttgacagat
     1801 ggcgagttgg cttcgtcaat caccaaaatc actggccaag aagctttgta tttttatgat
     1861 gccgttgcac caatcatcga attcgatacc atcgacatga atgtcgctta ctataaaaac
     1921 cgttatgaca aaggcgacgg aatgtatatc aattgtccga tggacaagga tgaatatgat
     1981 gcattttaca atgctttgat cgatgcaaaa tgtgctccta tccacgattt tgagatgaaa
     2041 gttttcgagg gttgcatgcc ggttgaagag atggcccgcc gggggtacga aacgctgctt
     2101 tacggtccga tgaagccagt gggtctggaa cgtgacgaaa agcgacctta tgcggtcgtc
     2161 caactgcgtc aggatgacct tgccgggact ctttataaca ttgtaggctt tcaaacacac
     2221 cttttatttt ctgaacaaaa aaaacttatt agaatgattc cagggctaaa agacgccaaa
     2281 attgtcagat atggagtaac gcacagaaac tcatatgtca aggcacctga agttttgttg
     2341 tcaacatacc aaatgaagaa caataacaac gtatttatag ccggacagct gagcggagtg
     2401 gaaggatatg tggagtctat agcttccgga ctggttgccg gaatcaacgc atacagactt
     2461 ttttcgagat ttgagccgct tagatttcct ttagaaacgg ccatcggctc gcaagcgaat
     2521 tatcttgagt ttgccagccc ttcgcaattt caacctatga acgccaattt cggccttttt
     2581 ccagcactag acatgaaata taaaaaacga gataaaaaag ggttactggc aaaaagaagc
     2641 atggaaacca tggaagaatt cataaaggcg gtgaaaccat gaccgacaag gaaataatag
     2701 cttcatacaa agactatctg tccgaacaaa gaaactattc tccccataca gtgactgcgt
     2761 atatggagga tgtagaatgc ttggaaaact ttcttatgtc tgaagacctc ggtaatttga
     2821 tgacagtcac cgaccgcatc gcacgcttct atgtatcgtc gcttcacggc cattatgatc
     2881 cccgctcgat cagaagaaag atcagcagtg tcagatcgct ttacaaactg ttagtggaca
     2941 gaggggagct cgatcgcaat cctttccaga atgtcgagtt acccaaggaa tccaaacctt
     3001 tgccaaaatt tgtttacgaa aaggaaatga ataatttcct ggaaagaatc gattcatcgt
     3061 ctcccatagg caaacgcgat caagccatct tcgagatcct ttacggatcc ggattaagag
     3121 tcagcgaatt agtcacatta aagataaggg acatagattt ttttaatcat actttgcttg
     3181 ttcacggcaa aggatccaag gatcgttatg tacctttcca tgaaacggaa aaggaaagaa
     3241 tcaaaatgta tttggaaaca gtccgtccgc ttttccgctc ccgcacagag gaagcggacg
     3301 aaggatatct gtttgtgaat tttcacggcg ggcatctgac cgaccgtggt gtccgcaaga
     3361 ttttggacac cgaactagcc aaacaggcat cgaccatgaa actgactccc catgccttca
     3421 ggcattcttt tgcaacccac cttttggaca atggtgtgga ccttagaaca gttcaggaac
     3481 tactcggcca tgtcagtttg tcaaccactc aaatctatac gaaagtatcc aaagagaaac
     3541 ttaaggaagt ttatatggct tctcacccga gagcgaaaat caaatgattg gcataaccat
     3601 catagcagtc ggatcgctca aagaagatta tttaaaagcg ggagtaaagg aatacataaa
     3661 gcgtatttcg gcatatgcca aactcaatat gatcgaaatt tcggataagt ctttgaacaa
     3721 gaatccaagt cagggagata ttcaaaaagt acttgatcaa gaaggtttgg aaatcctgaa
     3781 gaaaataaag tcttcgacat ttgtagttac gttggaaatt gatggagaca tgatgacaag
     3841 cgatcagttt gccaaattca tagaacacac gacgacatac aattcttccg aaattgtatt
     3901 tgtaattgga ggatcgtggg gtatatcgcg ggaagtaaaa caaaggagca ataaatcgct
     3961 gtcactttcc agtatgactt ttccgcatca attgacaagg atgatttttc ttgaacaact
     4021 atacagggca ttcacaatca taaacaatga gcc
//
LOCUS       DAOPJL010000059         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_27801_length_3688_cov_3.898431, whole genome shotgun sequence.
ACCESSION   DAOPJL010000059 DAOPJL010000000
VERSION     DAOPJL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27801_length_3688_cov_3.898431"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLP02_05855"
     CDS             <1..432
                     /locus_tag="PLP02_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPF42999.1"
                     /translation="YSDLHHLRPTDVSVNSLRADLDFGEATTLVSDTYGDVETYCYYS
                     SSYFEPRDEIKGDVARMLFYMAVRYEGESGEPDLELVNGTTTSDSSNIGDLATLLLWN
                     VADTVDAYEKSRDNLVYEYQHNRNPFIDHPEWVEIIFGGAA"
     gene            429..878
                     /locus_tag="PLP02_05860"
     CDS             429..878
                     /locus_tag="PLP02_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43000.1"
                     /translation="MMKKAFALIALISIAFLSGCIFDRTTSETTSSTTTSTTVFPTTT
                     TASDTASTTILTGSPTLTSDASINYVVGTDIILSFTLYDGEFVSLSGNGIEAADYQLV
                     GSQVTIVSEYVDSVFTNNPDQDVIVLSYKLSYSGGAVVGYLYIHNAS"
     gene            1049..1588
                     /locus_tag="PLP02_05865"
     CDS             1049..1588
                     /locus_tag="PLP02_05865"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HPF43001.1"
                     /translation="MMLKDIETLLKELKAEYRLIDTKDIIFRAEFLDKCKMNYCGRYN
                     RSWSCPPAIGDIGALDEKVLSYPKAVLFQTISLLEDNYDVDGMDAALRNMSKIALKLN
                     IMLNEKKADFMILSAGTCQICDKCTYPENPCRHPDLLQIPMEALGIDVGDLSGKYNLK
                     YYNGSNTVTYFSLCCFREN"
     gene            1643..2950
                     /locus_tag="PLP02_05870"
     CDS             1643..2950
                     /locus_tag="PLP02_05870"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HPF43002.1"
                     /translation="MKLIVSEKKLHDVLDFDDLRNLFRHFAKLTGIDVSLHDVEGLEI
                     LSYRIDPTKSICEQVKLKETSLNDCRLKMRYAGLKAAELGDPYIFQCGTMVKCSVPIF
                     FDEEFIGSLACGPVLLWDADDIARRDLVRFVENYDIPNQMAIEIIDCVKQLTPENMRS
                     AAKMLSVMVAYMCKEESIFQKQQAKLTKQQDKIADLMAERRVNAVSIDTIEKRNKFRK
                     YPFELEKELIAFLQTGDKQNAMKILNSILGEIFSQSSGNLDIIKANIYELTAVLMRAG
                     VDAGVALTDLSPILKIITRILSDGTEYDEVCLLTSEVMEEINHVISSNKFTKKSNENL
                     INAISYIKKNYRTDVSLDAVSKQVFVSSYYLSHLFRDELNMTFSDYVNKVRMDAAINL
                     MKETKMTIQEISEQCGFSDSGYFTRIFKKYHGITPKKYMSLYF"
     gene            3107..>3688
                     /locus_tag="PLP02_05875"
     CDS             3107..>3688
                     /locus_tag="PLP02_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPF43003.1"
                     /translation="MILDDISTLLQKGKAKDVKVLVQQALDEGIAPQKILNEGLIMGM
                     TVIGGKFKLNEVYVPEVLVAARAMNAGLEILRPALVLAGNKSVGKALICTVLGDLHDI
                     GKNLVKMMLEGQGIEVVDLGVDVAPDVIVNAIISEKPDIVCLSALLTTTMMNQKVIID
                     AIKEAGLRDQVKILVGGAPVTEAFAREIGADAYS"
BASE COUNT         1065 a          791 c          833 g          999 t
ORIGIN      
        1 tattccgatc tgcatcatct gcgcccgaca gatgtcagcg tcaatagctt gagagccgat
       61 ctcgactttg gcgaagcgac gactctggta agtgacactt acggagatgt ggaaacctac
      121 tgttactata gttcgagcta ttttgaacct agagacgaga taaagggcga tgtcgcccgg
      181 atgcttttct acatggctgt ccgttacgaa ggcgaaagcg gcgaaccgga tttggaactg
      241 gtaaatggta ccacgacttc cgattcttcg aatatcggcg atctcgccac cctgcttttg
      301 tggaatgtcg cggatacagt ggatgcttat gagaaaagcc gtgacaatct cgtctacgaa
      361 taccagcata accgcaaccc ttttatagac catcctgaat gggttgaaat catttttgga
      421 ggtgctgcat gatgaaaaaa gcatttgcgt tgatcgccct gatttccatt gcttttctgt
      481 cgggatgtat ctttgatcgg acaacttcgg aaaccacctc ttcaacaacc acaagcacga
      541 cagtatttcc aacgacaact accgcgagcg acacagcttc cacaacaatt ttaaccggaa
      601 gcccgacact cacatccgat gcctccataa attacgttgt cggaacggat attatacttt
      661 cttttaccct ctatgacggc gagtttgtgt ccttgtcagg caatggtatc gaagctgctg
      721 actatcagtt agtcggcagt caggtaacaa tcgtttctga atatgttgac tcggttttca
      781 caaataaccc tgatcaggat gtcattgttc tttcctacaa gttatcttat tccggaggag
      841 cagttgtcgg ctatttgtat attcataatg ccagctgacg atatccatag tctttcatta
      901 gtcttttgac ttatttagcc cgttttctgg tatttccaac aaaacgggct ttttcttttc
      961 ggtttctgac ttccgacttc gcctttcgcc ggacttcgtt gcttttgatt ttggcttgtg
     1021 ctaaaataca ttcgataaga aggaatgaat gatgctaaaa gatattgaaa cattactgaa
     1081 agaattaaaa gccgaatatc gcttgattga cacaaaagat atcatttttc gggctgaatt
     1141 cctggataaa tgcaaaatga attattgcgg aagatacaat cggtcctggt cgtgtcctcc
     1201 ggcaatcgga gacatcggcg cgcttgatga aaaagtgctt tcatatccga aagcggtctt
     1261 atttcagact attagtttgc tcgaggataa ttatgatgtc gacggtatgg atgctgctct
     1321 tagaaacatg tcaaaaatcg ctttgaaact gaatatcatg ctaaatgaaa aaaaagcgga
     1381 ttttatgatc ctttccgccg gcacttgcca aatctgcgat aaatgcacat atccagaaaa
     1441 tccttgtcgc catccggatc ttttgcagat accgatggaa gcactaggaa ttgatgttgg
     1501 cgatctatcc ggaaaatata atctcaagta ttataacgga tcgaataccg taacctattt
     1561 ttccctttgc tgtttccggg agaactagtg ggtttacagg aaacaatcta atttgagcca
     1621 tgacataaac ggagacgcaa atatgaaact gatcgttagc gaaaaaaaac tccatgatgt
     1681 ccttgacttc gacgatctga gaaatctgtt tcgtcatttt gccaaactga cgggaatcga
     1741 tgtttccctg catgacgtgg aaggattgga aattctttcc tatcggatcg atcccacgaa
     1801 aagtatttgc gaacaggtca aattaaaaga aacaagcctc aacgactgtc ggttgaagat
     1861 gcgttatgcc ggcctaaaag cggccgaact tggcgatccg tatatttttc agtgcggcac
     1921 catggtcaaa tgttccgtac cgattttctt tgatgaggaa tttatcggct ctctggcttg
     1981 cggtcccgtt cttttgtggg acgctgacga cattgccagg cgtgacttgg ttagatttgt
     2041 cgaaaattac gatattccca accaaatggc gatcgaaatc attgattgcg tcaagcaatt
     2101 gacgcctgaa aatatgcgta gcgccgccaa aatgctttcc gtgatggttg cgtatatgtg
     2161 caaggaagag tcgattttcc agaaacaaca ggccaaactc acgaagcaac aagataaaat
     2221 agccgatcta atggccgaaa gaagagtcaa cgccgtcagc atcgacacaa tcgaaaaaag
     2281 gaacaaattt cgtaaatatc ctttcgaact ggaaaaagaa ttgattgctt ttctccaaac
     2341 cggcgacaag cagaatgcca tgaagatcct gaattctatt ttaggagaga tattctcgca
     2401 atcgtccgga aaccttgata tcatcaaagc caacatctac gaactgaccg ctgttctcat
     2461 gcgggcgggc gttgacgccg gcgtcgcttt gaccgatttg agtccgattc tgaaaataat
     2521 taccaggatc ctttcagacg gcacggagta tgatgaagtc tgccttttga caagcgaggt
     2581 catggaagag atcaaccatg ttatttcctc aaacaaattc accaagaaaa gcaatgaaaa
     2641 tctgatcaat gcgatcagtt acatcaagaa aaactaccga accgatgtca gtctcgatgc
     2701 ggtctccaag caagtttttg tcagcagtta ctatctttcg caccttttcc gggacgagct
     2761 caacatgaca ttctccgatt acgtgaacaa agtgagaatg gacgcggcta tcaacctgat
     2821 gaaggaaact aaaatgacga ttcaggaaat atcggagcaa tgcggttttt ccgattctgg
     2881 ttattttacc agaattttca aaaaatatca cggaatcact ccgaaaaaat acatgagtct
     2941 gtatttttga gtatatgaaa ttggttataa ggcacaaccg tcaagagttg tgctttttct
     3001 ctataaaagc aatataatcc atttttaaaa caaagaaccc gcttataaat gtaagcgctg
     3061 ttaggtataa tatccttgta aaatgggaca aaggacggta attggtatga ttcttgacga
     3121 tatcagcacg cttttacaaa aaggaaaggc caaagatgtc aaagtcttgg tccaacaggc
     3181 tttggacgaa ggcatcgcgc ctcaaaaaat cctgaacgaa ggtttgatca tgggaatgac
     3241 ggtcatcggc gggaaattca agttaaacga ggtgtacgtg ccggaagttc tagtcgctgc
     3301 cagagccatg aatgccggat tggaaatatt gcgtccagcc cttgtcctgg ccggaaacaa
     3361 gtccgtgggc aaagctctga tttgcacggt tctgggtgat ttgcacgaca tcggcaaaaa
     3421 tctggttaaa atgatgctgg aaggacaggg gatcgaagtg gtcgatctcg gagtcgatgt
     3481 ggctcccgac gtgatagtga atgccatcat cagcgaaaag ccggatattg tttgcctttc
     3541 ggccctgctt accacgacaa tgatgaatca aaaggtgatc attgacgcga tcaaggaagc
     3601 cgggttacgc gaccaagtca aaatcctggt tggcggagca ccggttactg aagcttttgc
     3661 acgggaaatc ggagccgatg cctacagc
//
LOCUS       DAOPJL010000060         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_27837_length_3682_cov_5.627240, whole genome shotgun sequence.
ACCESSION   DAOPJL010000060 DAOPJL010000000
VERSION     DAOPJL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27837_length_3682_cov_5.627240"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PLP02_05880"
     CDS             <1..1277
                     /locus_tag="PLP02_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPF43004.1"
                     /translation="TNHMDIEIIEWLEDYLKKYRGAVVVITHDQYFINKVTNIIYELD
                     QETMSRYTGSYAEYEAEKVKRYELLMKQYSRQQKEIAHLQSFVDRFRYKAKKASQAQD
                     RVKKIARIERIDKPTKSEREISFAFRTPRPTDAIILEAENLTIGYDKPLIENITFKMR
                     GKDKIAIIGQNGAGKTTLVKTMLGTILPLYGEVEFHKQFKIGYFDQMSTENRMVGKLI
                     DIVHNLYPQKTLLDVRKLLARFQFIGEDAFKETGMLSGGEMVRLRLLLLLLESPEFLV
                     LDEPTNHLDISTKDIVEDVFAEYDGPILFISHDRYFINKVATRVIELDESKWQLYEGN
                     YDFYKESKENMTPIIKNREKRQKQLDPLKEIKKLETSLEENSKRISLLQKSLYQPEIY
                     MDKEKYDHAEAEIRLLETNSEDIMKRIEDLSI"
     gene            1356..3068
                     /gene="argS"
                     /locus_tag="PLP02_05885"
     CDS             1356..3068
                     /gene="argS"
                     /locus_tag="PLP02_05885"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPF43005.1"
                     /translation="MIDKIKSELQKMLEIKIRTRFNTDAQVVVEEPKKAGLGDISVPV
                     FSVAKAIGKPLPETVKIVREILAASEEAGLFEEISSVGGFVNLTLSKPEFAKAVMSEF
                     KKEGEMFGNSQVGGNKTICIDYSSPNIAKSFSIGHLRSTIIGHAIGNLMEKCSYNVVR
                     INHLGDFGTQFGKVIYAYQHFGSEEAVRANPVEELAKLYVEFHDRAEKDPSMDIEARK
                     IFKELEQGNPEYVKLWAWFREESLRDYLEVYDLLGVHFDSYNGEAFYNDKMAPVVDEL
                     EDKGLLQEDQGASVVFLGDDVPPALIKKSDGSTLYMTRDLAAVFYRKNTYKFDKVLYV
                     VGNEQKLHFEQLRRLIRLMGYPFSEDIVHVNFGLVLQDGKKMSTRKGKIVKLADVINE
                     AIQMSLEYIEEKNPDLENKAEIARKVGVSAIIFNDLKNYRANDFEFNLEEMVNFFGQT
                     GPYLQYTSVRINSILSDAPNFKFNIEVDPEWYKQEPVFAIIKQLGQYQATIEKSVAEY
                     APSILAKYLLNLASLFNSLYGLEKFLVDNEKEKHTKLHLLKMVKDVLDDGMKLLGMQT
                     IDKM"
     gene            3114..>3682
                     /locus_tag="PLP02_05890"
     CDS             3114..>3682
                     /locus_tag="PLP02_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPF43006.1"
                     /translation="METPVLDTKPKNRVGHSLVLMFKWMGKYWPLLIVSFAFLYIVSY
                     LRTLIPLFGQHIIDVILGYGTDGSKLPPFFQELITGDTVASKLLTAAIIMVAVQFTRS
                     VTIFIRRVVTAFFSEKVAYNIRDDLYKKLQNMDYNFHSHAETGDIIQRCTTDVETYKF
                     FIGDQIIEIMRLGLLVGLTIWQMSKLDHFL"
BASE COUNT         1151 a          822 c          831 g          878 t
ORIGIN      
        1 ctaccaacca tatggacatc gagataatcg aatggttgga agattatctg aaaaagtatc
       61 gtggcgcagt cgtcgttatc acgcatgacc agtatttcat caataaagtg accaatatca
      121 tatatgaact tgatcaggaa acgatgagtc gctacactgg gtcatatgcg gaatacgaag
      181 ctgaaaaggt caaaagatac gagttgctca tgaaacaata cagccgccaa cagaaggaaa
      241 ttgctcatct acaaagcttc gtcgaccggt ttcgctataa agcgaaaaag gccagccagg
      301 cccaggacag agtcaagaag atcgctagaa tcgaacggat cgacaaacct actaaaagcg
      361 aaagggaaat atcgttcgct ttccggacgc cccggccaac ggacgcaatt atcttggaag
      421 ccgaaaactt gacaatcggt tatgacaagc cattgatcga aaatatcaca tttaagatgc
      481 gtggcaaaga caagattgcc attatcggac aaaacggggc cggaaaaacc actctggtta
      541 agaccatgct cgggactata ctgcctttat acggagaagt ggaatttcat aaacaattta
      601 aaatcggtta tttcgatcaa atgtcgaccg aaaaccgtat ggtcggaaaa ctgatcgata
      661 tcgttcacaa cctctatccg caaaaaacct tgttggatgt cagaaaatta ctggcaagat
      721 tccagtttat cggagaagac gcgttcaagg aaaccggcat gttgtcgggc ggtgaaatgg
      781 tcagattgag attgttgttg ctattgttgg agtcaccgga atttctggtg ttagacgaac
      841 cgaccaacca tctcgacata tcaactaaag acatcgtcga agacgtgttc gcagaatatg
      901 acggtccgat attgttcatt tcccacgacc gttactttat caacaaggtc gccacaaggg
      961 ttatagaatt ggatgaatca aagtggcaac tctatgaagg aaactacgat ttctacaagg
     1021 aatcgaaaga gaatatgact ccgattataa agaataggga gaaaaggcag aaacagctcg
     1081 acccccttaa ggaaatcaaa aagcttgaaa catcactgga agaaaattcc aaacgtatct
     1141 cactgttgca aaagagttta tatcaaccgg aaatctacat ggacaaggaa aaatacgacc
     1201 atgcggaagc ggaaatcaga ctcttggaaa ctaactccga agatatcatg aaacgaatcg
     1261 aggacttgtc aatctgataa acccagattc gatagacatg aaaataaata ttattttggc
     1321 gctggttttt caaagacatg gaaagggtga tctaaatgat cgataaaatc aaatcagaac
     1381 tccaaaaaat gttggagatt aaaatcagaa cccgcttcaa tactgatgcg caggttgtgg
     1441 tagaagaacc gaaaaaggcc ggacttggcg atatttcagt ccctgtgttt tccgttgcca
     1501 aagcaatcgg caagccactt ccggaaacgg ttaaaattgt cagggaaatt ctggcagcct
     1561 cagaagaagc cggtctgttc gaagaaatct cgtctgtcgg cgggtttgtc aacctcaccc
     1621 tatcgaaacc ggaattcgcc aaggccgtca tgtcggaatt caaaaaagaa ggcgaaatgt
     1681 ttggcaactc acaagtgggc ggaaataaga ctatctgcat cgattactcg agccccaata
     1741 ttgccaagtc attttcaatc ggccatttgc gttcgacgat tattggacac gctatcggca
     1801 atctcatgga gaaatgctcg tacaatgtcg tcaggatcaa tcacttaggc gatttcggca
     1861 cccagttcgg caaggttatc tatgcctatc aacatttcgg ttccgaagaa gcggttcggg
     1921 caaatccggt cgaagaactg gccaagttat atgttgaatt tcatgaccgg gccgaaaaag
     1981 atccatcgat ggatattgag gcaagaaaaa tattcaagga acttgaacaa ggcaatcccg
     2041 aatatgtcaa actctgggct tggtttcgcg aagaatccct tcgcgactac ttagaggtct
     2101 acgacctttt gggagtccat ttcgattcct ataacggcga agccttctat aacgacaaaa
     2161 tggcaccggt tgtagacgaa ttggaagaca agggtttatt gcaggaagac cagggagctt
     2221 ccgtcgtctt cttaggcgac gatgttcctc ccgccctgat caaaaaatca gacggatcga
     2281 cgctttacat gacgcgtgat ctagctgcgg tgttctatcg caagaacacc tacaaattcg
     2341 acaaagtcct gtatgtcgtc ggcaacgagc agaaattgca tttcgaacag ctccgtcgcc
     2401 tcatcagact gatgggatat cccttcagcg aagacattgt tcacgtcaat ttcggtctgg
     2461 tgttgcaaga cggcaagaag atgtcgacca ggaaaggcaa gatcgtcaaa ctggcggacg
     2521 tcatcaatga agccatccag atgagcctcg agtatatcga ggagaagaac cccgatttgg
     2581 aaaacaaagc ggaaattgcc cgcaaagtcg gcgtcagcgc cattattttc aacgatctca
     2641 agaattaccg cgccaacgac ttcgagttca atctcgagga gatggtcaat ttcttcggcc
     2701 agaccggccc gtatctgcaa tacacttctg tcaggatcaa ttcgatcctt tcggacgcgc
     2761 ccaatttcaa attcaatatc gaagtcgacc cggaatggta caaacaggag ccggtgttcg
     2821 ccatcatcaa gcaactggga caataccaag cgacaatcga aaaaagcgtc gctgaatacg
     2881 ctccgtcgat tctggcgaaa tatctgctca atctggcgag ccttttcaac agtctttacg
     2941 gactggaaaa gttcttggtc gataacgaaa aggaaaaaca tacaaaattg catcttttga
     3001 aaatggtcaa ggatgtgctt gacgacggca tgaagttgct cggcatgcag acaattgaca
     3061 aaatgtaaaa tcacaaacga accttccata tccgagaaac gaggtgatat gatatggaaa
     3121 cccccgtgct tgacacgaaa ccaaaaaaca gagtcggaca ttctctcgtc ctgatgttca
     3181 aatggatggg caaatactgg cccttgttga tagtatcatt cgcatttctt tacattgtat
     3241 cttatctgag aactttaatc ccgctgttcg gacagcacat catagacgtt attctgggct
     3301 acggcaccga cggatccaaa ctgccgccct tcttccaaga attgataacc ggcgatactg
     3361 ttgcctcaaa acttctgacg gcggcaatca taatggtcgc ggtccagttt acgcgttcgg
     3421 tgactatttt tatccgtcgg gtcgttacgg cttttttctc tgaaaaagtc gcttacaaca
     3481 tcagagacga cttatacaaa aaattgcaga atatggatta taatttccat tcgcacgccg
     3541 aaactggcga cattatccaa agatgcacca ccgatgtcga aacatataaa ttctttatcg
     3601 gcgaccagat aatcgagatc atgcggctgg gcctgcttgt cggactgaca atctggcaga
     3661 tgtcgaaact cgatcatttt ct
//
LOCUS       DAOPJL010000061         3407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_31094_length_3407_cov_7.755668, whole genome shotgun sequence.
ACCESSION   DAOPJL010000061 DAOPJL010000000
VERSION     DAOPJL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31094_length_3407_cov_7.755668"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLP02_05895"
     CDS             <1..388
                     /locus_tag="PLP02_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF43007.1"
                     /translation="LAGAGAALYNLSGNTEFYWSTYQSLPAVGFNGIPVALLAYNNPI
                     GVIFSGIFMSYLNVAGLQLKNLTPFNEYITDIIIAAIVYMSAFSLVIKDILNKRNRKK
                     KDHDTIVGEAIADIPKDEIEKPEVTE"
     gene            388..1404
                     /locus_tag="PLP02_05900"
     CDS             388..1404
                     /locus_tag="PLP02_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF43008.1"
                     /translation="MAFLIQQTIIYTIPLMIVALAGVFAERSGIINIALEGLMVMGAF
                     IGVLFVRSIIQLDVPLSFGQPLLILGMLISTIGGCLFALLLGFAAINLKADQTIGGSA
                     LNLLAPALVLFFILLLTSQNVLNIDSRYSSNDYFMLNQDDFGLGTRLENPLGVIGNIL
                     FNKTYITTWICLALYIGLSIILYKTKLGLRLRSCGENPQASASVGINVYKMRYIGILI
                     SGGLGGLGGFVYSVTTVAASSNGDVAGFGFLALAVMIFGNWKPKNIAFAALLFGLLKC
                     IAVGYPYIDINNDGIYFLNTLGISPHIYRILPYLITLVVLAFTSKKSRAPKAEGIPYD
                     AGKR"
     gene            1600..2106
                     /locus_tag="PLP02_05905"
     CDS             1600..2106
                     /locus_tag="PLP02_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF43009.1"
                     /translation="MLIREALKADAEGKGLVHYQSWIETYTGLMDPVFLSKRSLERCV
                     NIAREHPENTIVAEIDGQIVGFACYSETRDKDMSDAGEIQALYVLKKYQKQGIGVALL
                     KRCEQILKQHPKIMLYVLAGNKNAIGFYEYNGFRFDGITKDMEVMPGIVLHEIRMVKD
                     RANDKQEE"
     gene            2109..3257
                     /locus_tag="PLP02_05910"
     CDS             2109..3257
                     /locus_tag="PLP02_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF43010.1"
                     /translation="MQYREFPSISGGKVSLLGFGCMRFPTTDPETSAIDESLSTAMID
                     YAYKHGVTYFDTAYPYHKGMSETFVGKTLKKYPRDSFRLATKMPLWLLKEKGDAERIL
                     AEQMERLQVSFFDFYLLHDMNRENYETAVKFGVIDLLAEYKRKGLIRNLGFSFHDKVD
                     VLEKHLQVREWDFVQLQINYLDWEMQDAKRAYELVKEKNIPVIIMEPVRGGALANLPK
                     RAAELLNKLDPSKSNASWAIRYAASKENALTILSGMSSMAQVEDNIETMKEFRPLNEK
                     ELNAINQAKSFLLASGAIPCTGCRYCMDCPSGVDIPRVFAIYNQYVMTNNKWSFLNSY
                     QRVLEDSKKADKCVACGKCARRCPQAIDIPGWMKKISEAVMEMQKPDK"
BASE COUNT          987 a          688 c          806 g          926 t
ORIGIN      
        1 attggccgga gccggagctg ctctttataa tttgtccggc aacacggaat tttattggtc
       61 gacctatcaa tcgctcccgg cagtcgggtt taacggaata ccggttgccc ttttggctta
      121 caacaatccg atcggggtta ttttttccgg aatattcatg tcttatctga atgtagccgg
      181 acttcaactg aagaatctga cgccattcaa cgaatacatt accgatatta tcatcgcggc
      241 aatcgtctat atgagcgcat tctcattggt tatcaaagat attttaaata aaaggaaccg
      301 aaagaaaaaa gaccatgaca ccatcgtggg cgaggcaatc gccgatattc cgaaggacga
      361 gattgagaaa cccgaggtga cagaataatg gcttttttaa ttcagcaaac gattatttat
      421 acaattccat tgatgattgt cgcgcttgcc ggagtgttcg cagaaagaag tggaatcatt
      481 aacatcgcct tggaaggttt gatggtcatg ggcgctttta tcggcgtcct gtttgtcaga
      541 agcattatcc aacttgatgt tccactttcg ttcggccaac ctttattgat cctgggcatg
      601 ttgatttcca ctatcggcgg gtgcttgttc gctctgttac ttggttttgc ggccattaac
      661 ttaaaggccg atcaaaccat cggcggcagc gccctgaacc ttttggctcc ggcattggtg
      721 ttattcttca tattattgct gacatcccaa aacgttctga acatcgattc cagatacagt
      781 tccaacgatt attttatgtt gaatcaggat gactttggcc tggggaccag gctggagaac
      841 cctctgggcg tgatcggaaa tatcttgttt aacaaaacct atattactac ctggatctgt
      901 cttgcgcttt atatcggatt gtctataatc ctgtataaga ccaaactggg attgagactg
      961 cgttcctgcg gcgaaaatcc ccaagcgtcg gcttcggtcg gaatcaacgt ttataaaatg
     1021 cgttatatcg gcatcctgat ttccggcgga ctaggcgggt tgggcggatt cgtttattca
     1081 gtcacaaccg ttgccgcatc aagcaacggt gacgttgccg ggtttggttt cttggcttta
     1141 gccgtcatga tattcggcaa ctggaagccg aaaaacatcg cttttgcggc tttgttgttt
     1201 ggcttattga aatgtattgc ggtcggttat ccatatatcg acatcaataa tgacggaata
     1261 tactttctga atacgttggg catcagtccc cacatttacc ggattctacc atatttgatc
     1321 acgttggtcg ttctggcctt tacttcaaag aagtcccggg cgccaaaagc cgaaggcatt
     1381 ccgtacgatg ccggaaaacg ataaaaacaa ttttagcgcc attagaagaa aggccgtcag
     1441 atttggcgac cttttttatg ccgatatttg gatatgcaag tgtattaggt attaacaaaa
     1501 actaattgaa tagaacctgt tttttcttag tatattcgct taccggtcaa ataagtgtat
     1561 aatcaagatg gaaaactacc gaaataagcg gaggcaaaca tgttaatacg tgaagcactg
     1621 aaggctgacg ccgaaggaaa aggattggtt cattatcaat catggataga aacatacacc
     1681 ggactgatgg atcccgtctt tctttctaaa cgttcactcg agaggtgtgt caacatagca
     1741 agagaacatc cggaaaatac aatcgtggcc gaaatagacg gacaaatagt cggatttgcc
     1801 tgttattccg aaacaaggga taaggatatg agcgatgctg gtgaaatcca ggcgctttac
     1861 gtattaaaaa aatatcagaa gcaaggaatc ggggtagcac tactgaaacg ttgcgaacag
     1921 atactgaagc agcatccgaa aatcatgctg tatgttttgg cgggaaacaa aaatgccatc
     1981 ggtttttacg aatataacgg atttcgtttt gacggcatca ccaaagatat ggaagtgatg
     2041 cccggcatcg tattacatga aattagaatg gtaaaagatc gggcaaacga caaacaggag
     2101 gaataaatat gcaatatcga gaatttccat ccatatccgg aggcaaggta tccttgcttg
     2161 gcttcggatg catgcggttt ccgacgacag atccggaaac ttcggccatc gacgagtcct
     2221 tgtcaacggc gatgatcgat tacgcctata aacacggcgt cacatatttc gatacagctt
     2281 atccttatca taagggcatg tcggaaacgt tcgtcggaaa aaccttgaag aaatatcccc
     2341 gcgattcttt cagactcgct acgaaaatgc ctttatggct cttgaaagag aagggcgacg
     2401 ccgaaagaat actcgcagag caaatggaaa ggttgcaggt aagttttttt gatttttatc
     2461 tgttgcatga catgaaccgg gagaactatg aaacggcggt taaattcgga gtcatcgatt
     2521 tgttggcgga atacaagaga aaaggcttga tcagaaactt gggtttttct ttccacgata
     2581 aagtcgatgt tctggaaaag cacttgcagg tccgcgaatg ggatttcgtt cagttgcaaa
     2641 tcaattatct ggattgggaa atgcaggacg caaaaagagc ttatgagctg gttaaagaaa
     2701 aaaacatccc cgtcataatc atggagcccg tcagaggcgg agcgctagcg aacttgccaa
     2761 agagggcggc agaattgctc aataaattgg atccttctaa aagcaatgca tcgtgggcaa
     2821 ttagatatgc cgcttcaaag gaaaatgctt tgactattct ttccggaatg tcttcgatgg
     2881 ctcaggttga ggacaacatc gagacaatga aggaattccg gccgttaaat gaaaaagaat
     2941 tgaatgcaat caatcaagca aaaagttttt tgttggcatc cggagccatt ccctgtaccg
     3001 gatgccgcta ttgcatggat tgtccttccg gagtagatat tcccagagtg tttgctattt
     3061 ataaccaata tgtcatgaca aacaacaaat ggtcgtttct taattcctat cagcgggttt
     3121 tagaagacag taagaaagcc gataaatgtg tggcgtgcgg caagtgcgct aggcgttgtc
     3181 ctcaggcgat cgacattccc ggatggatga agaaaatcag cgaagcagta atggaaatgc
     3241 agaaacctga taaataaaaa aacagagcca gaaaggccct gtttttaata tttgtcctgt
     3301 aaggctctga gaaacgcgat ggaatgcttg tcgactagaa ccagaaaatg gcgattgtcg
     3361 tagaaaaact gcagttctct tctcaatgtg atgatgacgt tggttat
//
LOCUS       DAOPJL010000062         3315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_32314_length_3315_cov_5.590491, whole genome shotgun sequence.
ACCESSION   DAOPJL010000062 DAOPJL010000000
VERSION     DAOPJL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32314_length_3315_cov_5.590491"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLP02_05915"
     CDS             <1..997
                     /locus_tag="PLP02_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPF43011.1"
                     /translation="EALANLDLTEFLIPSGVYYFAQAFGENRENIDFKVANDHPYLKE
                     VSMAGHSVIVSKDEKTVLLVENQSLWRRTLKLAGSYEEIAPYAFSYTDYKGIVIPSSV
                     TEIGAYAFSHVDTETITINANTTSIGEYMFYDASCEKIQIPDSIEEICEGAFLNTPNA
                     IINFPANIKTIGDWAFANSGFSGELALPEGLETIGDAAFNANAITSIVIPSTVTSMST
                     YLFANDGAVTHRNYVTRITFVSDNFIFSEDGFKQFLIQNYMNQGYGLDCDYKLTGSEV
                     IITIVLPNDPQEASHVERAFTYYWTEDKPVYDERVTGDYFFAFAEHWLVFEYNGE"
     gene            1020..2354
                     /locus_tag="PLP02_05920"
     CDS             1020..2354
                     /locus_tag="PLP02_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HPF43012.1"
                     /translation="MMKKIIFTALMFLIIFGVYGCVTTTDTTATAMNTDPGSTSGVTY
                     EKIALKNEYMVTGYEGSDTTVVISSTYDGIPVTTIRNINNSAITSIVLPDSITYVEIG
                     SLYNTGISELTIPKKLYYFAQAFSSPYASDFIDIDFIIPADHTYFKEAVICGRDAVVT
                     KDETVLIALENTADADETFILPSTYVEIASYACSRLSFGDITLPISVTKIGTSAFSKS
                     VFENLTVYGHLSDIGEGCFWSVVFDRIEFENSIDVIEYNAFCKASGQEVILPASVQEI
                     GPYAFYDSSIETIVLPEYVKYIRDRAFAYAGFAGELVLPEGLETIEQYAFSSNWLNSV
                     VLPSTLSELDVKAFYNYSTSAVSKDLLLTRVEFESLSFVESESDLMTFVYGFVNYSFF
                     MHLMYEGNDFTITIVMPTNTEEAERLEAAFQAVIDTYSSEWELYDNFVFETP"
     gene            complement(2434..3102)
                     /gene="plsY"
                     /locus_tag="PLP02_05925"
     CDS             complement(2434..3102)
                     /gene="plsY"
                     /locus_tag="PLP02_05925"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPF43013.1"
                     /translation="MFTKIGFVVIAYLIGSIPFSYLLGEWFKKEDIRKMGSGNLGTTN
                     AYRVFGKLIGTTVLLLDTLKSGLLVLLMKYTGIFDGIELFHPLVYGLASVLGHVFPIW
                     FKFHGGKGVASSFGLLLAYDPIMAAVILPIFLFVEFLTRYVSVASTVAALSALLFAVL
                     SYFFIEPDIYFPIITFLAVSLIIWRHLSNYKRLMNGCENRVGIFDWYDRLKEKRKTKK
                     HSKK"
BASE COUNT          977 a          712 c          724 g          902 t
ORIGIN      
        1 agaagcgctg gcaaacttgg atttgaccga attcttgatt ccttccggcg tctattattt
       61 tgcccaggct tttggagaaa accgggagaa tatcgatttt aaagtggcaa atgatcaccc
      121 gtatctaaag gaagtgtcga tggccgggca cagtgttatc gttagcaagg acgaaaaaac
      181 ggtattactg gtcgaaaatc aatcgctatg gagacgcacc ttgaaattgg ccggttccta
      241 tgaagaaatc gctccttatg ccttctctta tacagactac aagggaattg tgatcccgtc
      301 ttcggtgacc gaaatagggg cttacgcttt cagccatgtc gataccgaaa cgattacgat
      361 taacgccaac accactagta tcggcgaata catgttttac gatgcttcat gcgaaaaaat
      421 acagatacca gatagtatag aagaaatatg tgaaggcgca ttcttgaaca caccgaacgc
      481 catcatcaat tttccggcaa atatcaagac gatcggcgat tgggcttttg ccaattccgg
      541 gttttccggg gaacttgcat tgccggaggg cctggaaaca atcggggatg ccgctttcaa
      601 tgccaatgca atcacaagca tcgttattcc atcgacagtt accagcatgt cgacatacct
      661 tttcgctaac gacggggcgg taacacatcg taattatgtg accagaatca ctttcgtatc
      721 ggataacttc attttttccg aggacggctt caagcagttt cttattcaaa attacatgaa
      781 ccagggatat ggactcgact gcgattacaa attgactggt agtgaagtca tcataacgat
      841 tgttttgcca aatgatcctc aggaagcttc gcatgtggaa agagccttca cttattactg
      901 gacagaagat aaaccggttt atgacgaaag agtgacaggc gactatttct tcgcatttgc
      961 ggaacactgg cttgtttttg aatacaacgg cgaataaaat aatcaggaag tggggtatta
     1021 tgatgaaaaa aatcattttc acagcattga tgtttttaat tatattcggc gtttacggat
     1081 gcgtaacgac aacagacacc accgcaacag ccatgaatac cgatcccgga agcacatccg
     1141 gcgtaaccta tgagaaaatt gctttgaaaa acgaatatat ggtgaccgga tatgaaggca
     1201 gcgacacaac cgtcgttatt tcttcaacct atgacggtat cccggtaacg acaatccgca
     1261 atatcaataa tagcgccatt acgtccattg tgttgcccga ttcgatcact tatgtcgaaa
     1321 taggcagttt gtataacacc ggtatttccg agttgaccat tcctaaaaaa ctctattatt
     1381 tcgcccaagc tttctcttcg ccgtatgctt ccgatttcat tgatatcgat tttatcattc
     1441 ccgcggatca tacttatttc aaggaagccg tgatttgcgg ccgtgatgcg gttgttacca
     1501 aagatgaaac ggttttgatc gccctggaaa atacagctga cgcggatgaa acattcattc
     1561 ttccatcgac ctatgtggaa attgcttctt atgcctgttc cagattgtct ttcggggata
     1621 taacactgcc catatccgtg accaagatcg gcacaagtgc gttctcaaaa tccgtatttg
     1681 agaatctgac cgtgtatggg catttgagcg atatcggaga aggctgtttc tggtccgttg
     1741 ttttcgatag aatagaattt gagaattcaa ttgatgtcat tgaatataac gctttttgta
     1801 aagctagtgg tcaagaggtc atattgccgg catcggtaca ggaaatcggt ccatatgcct
     1861 tctatgattc ctcgatagaa acgattgtct tacccgaata tgtcaaatat attcgcgatc
     1921 gggcttttgc ctatgccggt tttgccggag aattggtttt accggaggga ttggagacta
     1981 tcgaacaata tgccttcagc agcaactggc tgaattcagt tgtcctcccg tccactttgt
     2041 cagaactgga tgtcaaggca ttttacaact atagtacttc ggctgtgtcc aaggatcttt
     2101 tattgaccag agtcgaattc gaatcgcttt cgtttgtcga atcggaatcc gacctgatga
     2161 cattcgttta tggatttgtg aattattcat tcttcatgca tcttatgtat gaggggaatg
     2221 acttcacaat cacaattgtg atgcccacaa acacagaaga agcggaaaga ttggaagcgg
     2281 cttttcaagc ggtcatagat acctattcca gcgaatggga attgtacgat aattttgttt
     2341 ttgaaactcc ttgatatgga tatataagat aaaaaaagtt agtagacacg tttataggac
     2401 gcgcttgcta acttttttta tttaggtatc agattatttt tttgaatgtt ttttcgtttt
     2461 ccgtttttct ttcaacctgt cgtaccagtc gaagatgcca actcgatttt cacaaccgtt
     2521 catgagccgt ttgtaattgg acagatgccg ccaaatgata agcgacaccg ccagaaaagt
     2581 gatgatcggg aagtagatgt ccggttcgat gaaaaaatag ctcagaacgg caaaaagaag
     2641 cgctgacaac gctgccacag tggatgccac cgaaacatat cgcgtcagaa attcgacaaa
     2701 caggaagatc ggcagaataa cagccgccat gatcggatca taagccagaa gcagaccgaa
     2761 cgaggaagcc actcccttgc cgccatggaa tttaaaccag atcggaaaaa catgtcccaa
     2821 tactgatgcc agtccgtaaa cgagcggatg gaaaagttcg attccgtcga aaatgcctgt
     2881 gtatttcatc aatagtacca acaacccgct tttcaaagta tccaaaagca ataccgtcgt
     2941 cccgatcagc tttccgaaga cgcggtaggc attggtggtg ccgagattgc cgctgcccat
     3001 cttgcggatg tcctctttct tgaaccattc ccccaaaaga taagagaaag gaatcgaacc
     3061 gataagataa gcaatgacta cgaagccaat ttttgtaaac atctaatgta acctccgcaa
     3121 tatggaaaat tatatcataa aaagcggaaa tttaaaggaa aaacattata aaatttgata
     3181 taatggataa agagcgaaaa aaggagcgat gtccatgaag aacccaatta ccgaaacgta
     3241 taccgctcaa tccctccaga ttctcgaggg attggaagca gtccgcaaac ggcccggcat
     3301 gtatgtcggc tccac
//
LOCUS       DAOPJL010000063         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_33566_length_3224_cov_7.193436, whole genome shotgun sequence.
ACCESSION   DAOPJL010000063 DAOPJL010000000
VERSION     DAOPJL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33566_length_3224_cov_7.193436"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2394)
                     /gene="parC"
                     /locus_tag="PLP02_05930"
     CDS             complement(<1..2394)
                     /gene="parC"
                     /locus_tag="PLP02_05930"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPF43014.1"
                     /translation="MAAKSAKQKIDVFLNEKIVTENLEAIVGDRFGKYSKYIIQDRAL
                     PDVRDGLKPVQRRILFAMQQLGMFSDKPYKKCARIVGEVIGKYHPHGDQSVYDAMVRM
                     SQPWKMGALLVDMHGNNGSIDNDPAAAMRYTEARLTPYAEMLLKDIEKKTVYFVPNFD
                     DEEMEPVVLPAKFPNLLVNGAMGMATGYATNIPPHNLNEVIQAVIYKIDHPSATVAEL
                     MTIIKGPDFPTGAIVEGTDEIEKAFSTGRGRIVLSSKVEIKDTKLIITEIPYEVNKAD
                     LVRKIDDVRSKKKIDGILEVRDESDREGLRIVVDIKKEISSEVILAYLLKNTELSVSY
                     NYNMVAIDNKSPRQMSLTDILDSYIIHQKEVVLNRSNFDLIRAEKRRHIVEGFLKMVD
                     ILDDVIKTIRASEGRKDAISKLMGTFGFTENQSEAIVNLQLYRLSSTDVVEMQKEATD
                     LQKQINYLNRILKNEKELENVIKTEISEIAEKYPVERKTQIREEIRKVEIDEQELFEH
                     DDVIVVLTGDGYLKRSSLKSYQASNGLPGLKQGDHVLKQEIVNTRDTMLLFTNLGNYI
                     NIPIFKIADTKWRDNGEYIGNYAQLNDQEEVIDYLVISDFETDQMVLLMTEEGQIKQV
                     PLSEFQQVRLNKTYSCMGASKINRMVSVDLREKYDTDIVVVTENGYVLKYLESEVPIQ
                     SLAARGVKAINLREDATIGARYLSMINKDEILLLSNRGGLKREFSQNIERSHRPAKGK
                     AYLKSVKSNPYKFVGFVAENIFRLKDYLDIRIMAKDKDLVIPGADLKPDRYENGIPYF
                     "
     gene            complement(2394..>3224)
                     /locus_tag="PLP02_05935"
     CDS             complement(2394..>3224)
                     /locus_tag="PLP02_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPF43015.1"
                     /translation="TASVLIEKAMNARNAREAARKAREDARLVKKVAKQETILSGKLS
                     PATTKNPKINELFLVEGDSAGGSAKQGRNRFFQAILPLRGKVINSEKQRMEDVLKNEE
                     ISTIISTIGAGLGTDFQVNKSNYNKVIIMTDADTDGAHIQVLLITFFFRYMRPLVEAG
                     KLYIARPPLYKIAKVGKKEEISYAWSDEEREEISKKYKTYTIQRYKGLGEMNAEQLWD
                     TTMNPETRTLVQVRIEDMADAEQRITVLMGDKVEPRKEWIDTFVSFESEDDFRRKEED
                     "
BASE COUNT          693 a          762 c          767 g         1002 t
ORIGIN      
        1 aaaataggga atgccgttct cgtagcgatc tggtttcaga tcggctcccg gtatcacgag
       61 atccttgtct tttgccatga tcctgatgtc aagataatct ttcagacgga aaatgttttc
      121 ggccacgaat ccgacgaatt tgtaaggatt gctcttgaca ctcttcaaat atgccttgcc
      181 tttggccggg cgatgacttc tttcgatgtt ttgcgagaac tcccgtttga gcccgccgcg
      241 gttcgataaa agtagaattt cgtctttgtt tatcatcgac aagtatctgg caccgatggt
      301 tgcgtcttcg cgtagattga tggctttgac gccgcgggcg gccagacttt ggatcggcac
      361 ttccgattcc agatacttaa gcacatagcc gttttcggtc acgacaacaa tatccgtgtc
      421 gtatttttcc cttaaatcga ctgataccat ccggttgatt ttggaagcgc ccatgcaact
      481 ataggtcttg ttcagacgga cctgttggaa ttcagataac ggtacttgtt tgatctggcc
      541 ctcttcggtc attagcaaaa ccatctgatc ggtttcaaag tcggaaataa caagataatc
      601 gatgacttct tcttgatcgt tcagttgcgc ataattgccg atatattcgc cattgtcacg
      661 ccatttggta tcggcgatct tgaagattgg tatgttgata taattgccta aattggtgaa
      721 taaaagcatt gtatccctgg tgttgacaat ctcctgtttc aggacatggt ctccctgttt
      781 aagacccggt aaaccgttcg aggcctgata ggacttcagg gatgagcgct taaggtaccc
      841 atcgccagtc agcacgacga tgacatcgtc atgctcgaaa agttcctgtt cgtcgatttc
      901 gactttacgg atttcttctc tgatctgggt tttacgttcg actgggtatt tttctgctat
      961 ttcgcttatt tcggtcttga tgacgttttc cagttccttt tcgtttttca aaatccggtt
     1021 cagatagttg atctgttttt gaaggtcagt cgcttctttc tgcatttcaa cgacatcggt
     1081 cgaggataaa cggtataatt gcaggttgac gatcgcttcg gattggtttt cagtgaatcc
     1141 aaatgttccc atgagtttgg aaatggcatc cttgcgtcct tcggaagcgc ggatcgtttt
     1201 gatgacatcg tccaggatgt cgaccatttt cagaaaacct tcgacgatgt gccggcgttt
     1261 ttccgctctg atcagatcaa aattggaacg attgagaacg acttcctttt gatggatgat
     1321 ataactgtcc aaaatatcgg tcagagacat ctggcgcggg gacttgttgt cgatcgcgac
     1381 catgttgtag ttataagaaa cgctcaactc ggtgtttttc aaaagataag ccaggatgac
     1441 ttccgaggaa atctcctttt tgatatcgac gacgattcgc aatccttccc ggtccgattc
     1501 gtcccttact tccaggatgc cgtcgatttt cttcttcgac ctgacatcgt cgatcttcct
     1561 caccaaatcc gctttgttga cttcgtaagg aatttcggtg ataatcagtt tcgtgtcttt
     1621 aatttctacc ttcgaggata acacgatccg gcctctgccg gtggaaaaag ccttttcgat
     1681 ctcgtcggtt ccttcgacaa tggctccggt cggaaaatcc gggcctttga tgatcgtcat
     1741 gagttccgcc acggtggcgc tcggatgatc gatcttgtag atcacggctt gaattacttc
     1801 gttcaggttg tggggcggga tattggtggc atacccggtt gccatgccca tcgctccatt
     1861 aacaagcaaa ttcggaaatt tggccggcaa aacaaccggc tccatttcct cgtcatcaaa
     1921 gttgggaaca aaataaacgg tcttcttctc gatgtcctta agaagcattt cggcataagg
     1981 agtcaatctc gcttcggtat aacgcatggc ggcggcggga tcgttgtcga tggagccgtt
     2041 gtttccgtgc atatccacaa gcaaagctcc cattttccaa ggttggctca ttctgaccat
     2101 ggcatcatat actgactgat cgccgtgcgg atgatatttg ccgatcactt cgccgacgat
     2161 acgggcacat ttcttgtacg gtttgtccga gaacatgcca agctgttgca tggcgaaaag
     2221 aatccggcgt tggaccggtt tcaaaccgtc cctgacatcg ggaagcgccc gatcctggat
     2281 gatatatttg gaatatttac cgaagcggtc tccgacgatc gcttccagat tttccgtgac
     2341 aatcttctcg ttcaggaaga cgtcgatctt ctgtttggcg gatttggctg ccatcagtct
     2401 tcctccttgc gcctgaaatc gtcttcgctt tcaaaagaaa cgaaggtgtc tatccattcc
     2461 ttgcggggtt ctaccttgtc gcccatcagg acggttatcc gttgttcagc atcagccatg
     2521 tcttcgatcc tgacctgtac gagcgtcctg gtttcgggat tcatcgtcgt gtcccaaagc
     2581 tgttcggcat tcatttcgcc caggcctttg tagcgctgga tggtgtatgt tttgtatttt
     2641 ttggagattt cttccctttc ctcgtcactc caggcatagg agatttcttc tttttttccg
     2701 acctttgcta ttttatatag tggcggcctg gcgatataaa gtttaccagc ttcgaccaaa
     2761 ggacgcatat aacggaaaaa gaaagtgatc agaagaacct ggatgtgggc gccgtcggtg
     2821 tccgcatcgg tcatgatgat tactttgttg tagttggatt tattgacctg aaagtcggta
     2881 ccgagaccgg ctccgatcgt ggaaatgatg gtggatatct cctcgttttt taagacatcc
     2941 tccattcttt gcttttcgga gtttataact ttgccgcgaa gaggcaatat ggcctgaaag
     3001 aaacggtttc ttccctgttt tgccgaaccg ccggcggaat cgccttcgac taagaaaagt
     3061 tcgtttatct tcggattttt cgttgttgcc ggggacagtt tcccggaaag aatcgtttcc
     3121 tgtttggcga ctttcttgac gagtctggca tcttccctgg cttttctggc agcttccctg
     3181 gcattgcggg cgttcatggc cttttcaatc agaacggatg ctgt
//
LOCUS       DAOPJL010000064         3104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_35390_length_3104_cov_8.983929, whole genome shotgun sequence.
ACCESSION   DAOPJL010000064 DAOPJL010000000
VERSION     DAOPJL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35390_length_3104_cov_8.983929"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..683)
                     /gene="rplA"
                     /locus_tag="PLP02_05940"
     CDS             complement(3..683)
                     /gene="rplA"
                     /locus_tag="PLP02_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPF43016.1"
                     /translation="MAKKGKKYTEALTKIERGKIYPVLEAVELVKAIAFEKFDATVEL
                     AMNLNLDPRKAEQNLRGALVLPHGTGKEKRVLVFARGDKAKDAKDAGADYVGDEDLVQ
                     KIQEGWFDFDVVIATPDMMGVIGKLGRVLGPKGLMPNPKTGTITQDVTKAVSESKGGK
                     ITYRVDKGGNIQAIVGKVSFKPEALVENIKAVTDVIMKAKPSTVKGVYLKNVAISSTM
                     GPGVDIEI"
     gene            complement(761..1186)
                     /gene="rplK"
                     /locus_tag="PLP02_05945"
     CDS             complement(761..1186)
                     /gene="rplK"
                     /locus_tag="PLP02_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPF43017.1"
                     /translation="MAKEIKTIVKLQIPGGKATPAPPIGPALGQAGINIQQFCVQFND
                     RTKEQVGSIIPVVITVFEDRSFTFITKTPPASDLLKKACKIQKGSGNSKQDKVATITR
                     AQLREIAAVKMPDLNANDIEAAARIIEGSAKNMGIVVVD"
     gene            complement(1323..>3104)
                     /locus_tag="PLP02_05950"
     CDS             complement(1323..>3104)
                     /locus_tag="PLP02_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF43018.1"
                     /translation="LEHVDSRNVYLLDLEKEENKEPVDLEFYYEKKPENKPSVKIISI
                     NNTVYIKADASQKVKYITDDSEIKLINDHYRKPETKDAMQHRFDLQQFGRIDNDGQRK
                     SFIRFKDTLKDGFHKTFRRRKFFSKLFMLGYLIISALIVYNLATFSNRFTISDSDFLD
                     TAKGMVVIKADDQFTAETMDQILLTPGITGFNFYRNRQAFTFLYGNFFQGYTNMSYYS
                     ISSNMETLYPVRLSSLGDITPLLGNVPADNTEIAIDKWIADDILENVYFKNMGGKTYS
                     DIIGIKITPYSGTHQLEIVGIVDTGSPIGVVTDDNLSIFYYNYEYLAGTYAQGTVDGY
                     YTITDGRDIQADGEILVASYNTMDIGSTYYGPFDQQHTIVGKFTSETLSGAQIVSTSD
                     FEDTILQILITNMGEREYYYESDYYAYIFEYYLTTDDVEQAVTDLDAAGFEVYDSYLK
                     ARTDYKTELWADNASKLRTILISLVGTLVYLVIMMRSSLLGRVKEVGIYRSIGATKKD
                     VGKIFISEIIAFTTVASMTGYLIMSGLIAEFIKMNPLGSGDFNFPFYIFLGGIVGIYL
                     INCVFGMLPVYSLLRKTPAEIGAKYDI"
BASE COUNT          707 a          712 c          762 g          923 t
ORIGIN      
        1 attcagattt cgatgtctac gccgggtccc atggttgagg agatggcgac gtttttcaga
       61 tagactccct tgacggtcga aggtttcgcc ttcatgatga catctgtgac ggctttaatg
      121 ttttccacca aggcttccgg tttgaatgac acttttccga cgatggcttg gatgtttccg
      181 cccttgtcaa cgcggtaggt gatcttgccg cccttggatt cggacacggc tttggtcaca
      241 tcctgggtga tcgtgccggt cttggggttc ggcatcaaac cttttggtcc gagtacgcgg
      301 cccagtttgc cgataacgcc catcatgtcg ggagtggcga tcacgacatc gaagtcgaac
      361 cagccttcct ggattttctg gacgagatct tcgtcgccga cataatcggc tccggcatcc
      421 ttggcgtctt tggccttgtc gccgcgggcg aaaacgagaa cacgtttttc cttgccggtt
      481 ccgtggggaa gcacaagggc tccgcgcagg ttttgctcgg ctttgcgggg atcaagattc
      541 aaattcatgg cgagttcgac ggtggcgtcg aatttctcga atgcgatggc tttgacaagt
      601 tcgacagctt ctaggacggg atatattttc ccgcgttcga tcttggtcaa ggcttcggtg
      661 tatttttttc ctttttttgc cattttgtta tggtttcctc ctatatctgg tattagcgga
      721 ttgtcctccc atatatagtt gcttgtaatc gattatagat tcagtcaact acgacaattc
      781 ccatgttttt ggcggaacct tcgatgattc tcgccgcggc ttcgatgtca tttgcattga
      841 gatccggcat tttgactgcc gcgatttctc tcaactgggc tctggtgatc gtcgctacct
      901 tgtcttgttt ggagttgccg gagccttttt ggattttgca ggctttcttc agaagatcgc
      961 ttgccggcgg cgtcttggtg atgaaggtga acgaacggtc ttcgaatacc gtgatgacga
     1021 ccggaatgat gcttcctacc tgttcctttg tgcgatcatt gaattgaacg cagaattgct
     1081 ggatgttgat gcctgcctgt cccagagcgg gaccgattgg gggagccgga gtggctttgc
     1141 ctcccgggat ttgcaacttg acgatggttt tgatttcttt agccacgaaa aacacctcct
     1201 tcttttcgtg atgtggtcaa ttggactttg tcctcccacg ccattatgtt atgcacacgc
     1261 tgaaatatta tacaataacg acacttcccg tgtcaaggtt tttttcctga caaaacgaga
     1321 acttaaatat catatttggc gccaatttcc gcaggcgttt tcctgagcag gctgtagacc
     1381 ggcaacatgc cgaaaacaca gttgatcagg tatatgccga caatgcctcc taggaagata
     1441 tagaacggga aattgaagtc tccggaacca aggggattca tcttgatgaa ctcggctatc
     1501 aagccggaca ttatgagata tcccgtcatg gaagcgaccg tcgtaaaggc aatgatttcc
     1561 gagatgaaga tctttcccac gtccttcttc gtggcaccga tcgagcggta aattccgact
     1621 tccttgactc tgccaagaag actggaccgc atcattatta caagatatac cagcgtccca
     1681 acaagcgaaa tcagaatggt ccgcaactta gaggcgttgt cggcccacaa ttccgtcttg
     1741 taatcggttc tggctttcaa atacgaatca taaacttcga atccggcggc gtcaagatcg
     1801 gtgactgcct gttcgacgtc gtccgtcgtc aagtagtatt cgaaaatata tgcgtaataa
     1861 tccgattcat aataatattc gcgttcgccc atgttggtga tgagtatctg caggatcgtg
     1921 tcttcgaaat cggacgtgga gactatttgc gcaccggaaa gcgtttcgga agtgaatttt
     1981 cctacgatgg tgtgctgctg gtcgaaggga ccgtaatatg tggaaccaat atccatcgta
     2041 ttgtaactgg cgacaaggat ttccccgtcg gcctggatat cgcggccgtc tgtaattgta
     2101 taataaccgt cgaccgttcc ttgtgcgtag gtgccggcca ggtactcgta attgtaatag
     2161 aatatggaga gattgtcgtc cgtcaccact ccgattggcg atcctgtgtc gacaattccg
     2221 acgatctcaa gctgatgggt accggaataa ggcgtaatct ttattccgat gatatcgctg
     2281 tatgtctttc ctcccatgtt tttgaaatac acgttctcca gaatgtcgtc ggcaatccat
     2341 ttgtcaatgg ctatttccgt attgtcggcg ggaacatttc ccaaaagagg cgtgatatca
     2401 ccgagcgacg acagtcttac cggataaagc gtttccatat tgctggatat gctgtaatac
     2461 gacatgttag tgtaaccctg gaagaaattg ccgtacagga aagtgaacgc ctgcctgttg
     2521 cggtaaaaat tgaatccggt tatccccgga gtgaggagta tctgatccat ggtttcggcg
     2581 gtaaactggt catcggcctt aatcaccacc atgcctttgg ccgtatcaag aaaatcactg
     2641 tcggatatcg tgaaacgatt ggagaaagtg gcaaggttgt atacaatcaa agcagagatg
     2701 atcaaatagc ccagcatgaa taatttcgaa aagaatttcc gccggcggaa ggtcttatga
     2761 aatccgtctt tcaaagtatc tttgaatcgt atgaaggatt ttcgttgtcc gtcgttgtct
     2821 attcttccga actgctggag atcgaaacga tgctgcatgg catccttcgt ttccggtttg
     2881 cgataatgat cattgatcaa tttgatttcc gaatcgtcgg tgatatattt tactttctgc
     2941 gatgcgtcgg ctttgatata aaccgtattg tttatggaaa tgatctttac cgaaggtttg
     3001 ttttccggtt tcttttcata ataaaattct aaatcgacag gttccttgtt ttcttccttt
     3061 tccagatcaa gtagatatac gtttcgcgaa tcgacatgtt ccaa
//
LOCUS       DAOPJL010000065         3060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_36106_length_3060_cov_5.731115, whole genome shotgun sequence.
ACCESSION   DAOPJL010000065 DAOPJL010000000
VERSION     DAOPJL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36106_length_3060_cov_5.731115"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="PLP02_05955"
     CDS             complement(<1..1895)
                     /locus_tag="PLP02_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPF43019.1"
                     /translation="MKKLVAAFLMAIFGFFVMFGNISHVQAEDNTIRIWFSQYEEENT
                     AMETIAAAFTDETGINVEVISRINIFNAASDLVNNAALDERPDIVFMQAPDIGSLVVN
                     GYLEPLTEYVAGTDLENRFVSVAFDAFSLDGELYGIGYSVDSYGLIYNKDIISKEELP
                     ATWEDFFDLAIQKTTYDTEGNVLVHGTLLNSRDMWFTYPIIRNYGGYYYGNYSNGDYN
                     PYDVGLDSEGMLDYIAKMKWAMENDLVLTNKIKGESEIVSQFANGTIGMIIYGLWYAE
                     TFQQKGINYGIASLPDQDNGETSLALTTVQGFVLNSYSLHKDESLSFLSYLLEDENQQ
                     LLIEAGNKYDSKVGTRNPADIAVIQSDYIQSSEILASLSALNDECEPFPNIPEGSIWY
                     NYTTSAFQSIFFDEGVDPMTKLQELADKIRDDVRLMNYQSEPIEIPEWLYYAFGAIIL
                     AGIGLAIFLKRKKEKKLPYLMKPKWRTTLLAWGLMTPLLILLMIFYVFPIFHNFYLSL
                     TNYSGINLRDYQLIGLANYYDIFVSGIGGLLSMTVWTLVFAFSVVAISFVFGSLLASI
                     LNSQKVKIAKIYRIIFILPWVVPTVITLLMWQGLLETEGGLVNQILGLFGIPNIPWLS
                     DSFLAKIS"
     gene            complement(1920..2996)
                     /locus_tag="PLP02_05960"
     CDS             complement(1920..2996)
                     /locus_tag="PLP02_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPF43020.1"
                     /translation="MFKDIAYYKPDIDKTFRESFKLYDDALHKKTGNVSFRVLPPSDV
                     EFYEDIDLTAFDYENKQDEYADAIVELLAQSYEKRKNLDDNMIPIVFPILGIGDYSAF
                     VAGDIVFKPETSWSEPVLKEIDDWKKLPPFGESPWYRRFLSISEKLIARSVKQGIPYM
                     RGFFSPLDLAHALRGADIYYDFYDEPEKLHDLLDYCADAIICFASDIYKLVETAYKGT
                     RYGNWYTKNMINMSEDIACMISGEAYREFCRPHTQKVINHFGIGYMHCHSRAMYLVKE
                     ICQMDNVVHLWLATDPNQPRPFDHLEQLVEDANGAVMAIDCDNFAEIENNISILKKGN
                     FSICLPVASIAEGEELIKRFKQLF"
BASE COUNT          859 a          673 c          677 g          851 t
ORIGIN      
        1 gatattttgg caagaaaact gtcggacagc caaggaatgt tcggaatacc gaataagccc
       61 aaaatctggt tgacaagccc gccttcagtt tctagcaatc cttgccacat caataaggtt
      121 ataaccgtgg gaacgaccca aggcagaatg aagatgatcc gatagatttt cgctattttt
      181 accttctggc tattcaagat tgaagccaaa agggaaccaa acacaaaaga tatagcaaca
      241 acggaaaaag caaatacaag cgtccataca gtcattgaca ataaaccgcc gatgccagaa
      301 acgaaaatat cgtaatagtt cgccagacca atcaattgat aatcccggag attgatgccg
      361 ctgtagttgg tcaatgaaag ataaaaatta tggaagatcg gaaatacata aaaaatcatc
      421 aaaagaatga gcaacggggt catcagtccc cacgccaaca aagttgttct ccacttcggc
      481 ttcattaagt atggcagctt cttttccttt tttcttttta ggaatatggc aagaccgata
      541 ccagccagga tgatggctcc aaaggcataa tacaaccact cgggtatctc gatcggttcg
      601 gactggtagt tcatcagtct gacatcgtcg cggatcttgt cggccagttc ctgaagttta
      661 gtcataggat cgacgccttc gtcgaagaaa atgctttgaa aagcactggt ggtgtagtta
      721 taccaaatgg aaccctcggg aatattcggg aatggttcgc attcatcatt cagggcggaa
      781 agtgacgcca gaatctcgga agactggatg taatcgcttt ggatgacggc gatgtcggca
      841 gggtttctgg ttccgacttt ggaatcgtat ttgttgccgg cttcgatcag aagctgttgg
      901 ttttcgtctt ccagcagata tgacagaaaa cttagacttt catctttgtg caaagaataa
      961 ctgttaagaa cgaatccttg tacggtggtc agagccagcg aagtttcacc gttatcctga
     1021 tccggcaggg aagcgattcc gtaatttata cctttttgct ggaatgtttc ggcataccag
     1081 agaccgtaga taatcattcc gatagtgccg ttggcgaact gcgaaacgat ttcgctctcg
     1141 cctttgatct tattggtgag caccaaatcg ttttccatcg cccatttcat tttagcaatg
     1201 tagtcgagca tgccttcgct atccagaccg acgtcataag gattgtaatc gccatttgaa
     1261 tagtttccgt aataatagcc accatagttg cggataatcg gataggtgaa ccacatgtcg
     1321 cgtgaattga gcaaggtccc gtgaactaag acatttcctt ccgtgtcata ggtcgttttt
     1381 tgaattgcta agtcaaaaaa atcttcccaa gttgctggca gttcttcttt ggagatgatg
     1441 tccttgttat agatcaaacc gtaagaatcg acggaatatc cgatcccgta aagttctccg
     1501 tccagactaa aagcatcgaa ggcgacggaa acaaaccggt tttccagatc tgtacctgct
     1561 acatactcgg tcaatggctc aagatagccg ttgacgacga ggctgccaat atcgggggct
     1621 tgcatgaaaa caatgtccgg acgttcatct aacgctgcgt tgttaaccag atcgctggcg
     1681 gcgttaaaaa tgttgatccg cgatattact tcaacattga ttccggtttc atcagtgaaa
     1741 gcggccgcaa ttgtttccat ggccgtattt tcctcttcat attgggaaaa ccaaattctg
     1801 attgtgttgt cttccgcttg aacatgggaa atgtttccga acatgacaaa aaagccgaat
     1861 attgccatga gaaaagcggc aaccagtttt ttcatcggtc gaaaacctcc tttccaggat
     1921 cagaacaact gtttgaagcg cttgatcaat tcttcgcctt cggcaatgga cgccaccggc
     1981 agacaaatag aaaaattgcc ttttttcaat atcgatatgt tattttcaat ttcagcgaaa
     2041 ttgtcacaat caattgccat aacggctccg ttggcgtcct cgaccaattg ttcgagatgg
     2101 tcgaaaggac ggggttggtt gggatcagtg gccagccaca gatgaacgac attgtccatc
     2161 tgacagattt ccttgacaag atacatcgct ctggaatggc aatgcatgta tccgattccg
     2221 aagtgattaa tcactttttg tgtatgcgga cggcagaatt cccgataagc ctcacctgaa
     2281 atcatgcagg cgatgtcttc ggacatgttt atcatgtttt tggtgtacca gttgccgtac
     2341 cttgtacctt tgtatgctgt ttctaccagt ttatagatat ccgatgcaaa acagatgatg
     2401 gcatcggcgc aataatccaa gagatcgtga agtttctccg gctcgtcata aaagtcgtaa
     2461 tagatatcgg ctccacgcaa tgcgtgggcg agatcaagcg gcgaaaagaa tccacgcata
     2521 tagggaatcc cctgtttcac acttctagct atcaactttt cggaaataga taaaaaccga
     2581 cgataccagg ggctttcacc aaacggtgga agttttttcc aatcatcgat ttcttttagt
     2641 accggttcgc tccaactggt ttccggtttg aatacaatat ccccagctac aaatgccgaa
     2701 taatcaccga ttcctaaaat aggaaacaca atcggaatca tgttgtcgtc gagatttttt
     2761 cttttctcat aggattgcgc aagcaattcc acaattgcat ccgcatattc gtcctgtttg
     2821 ttttcataat caaaggcggt caaatcgata tcttcgtaaa attccacgtc ggatggtggc
     2881 aaaacccgga aagagacgtt tccggttttt ttgtgaaggg catcatcata taatttgaat
     2941 gattcacgga atgttttatc gatatccggt ttgtaataag caatatcctt gaacatatta
     3001 ttttgcctcc gtcaggtaat gaagagctac ttcggcggcc gtggcggcat cgggactgta
//
LOCUS       DAOPJL010000066         2753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_41951_length_2753_cov_6.514085, whole genome shotgun sequence.
ACCESSION   DAOPJL010000066 DAOPJL010000000
VERSION     DAOPJL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41951_length_2753_cov_6.514085"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..817
                     /locus_tag="PLP02_05965"
     CDS             284..817
                     /locus_tag="PLP02_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPF43021.1"
                     /translation="MTIDFDKYIDLLKEKDNEAFAFVYENTKRGVFSVIAPIVRDKSE
                     VEDLMQDTYIKMLQNINSYQKGRNFTAWLFQIAKNLAIDYYRKQKRTTVLDPQDHEYV
                     FDKEQETNEDYGLEINEVIGILSPEEKEIVLLKIVSGNVFREIADIVDKPLGTVLWIY
                     NKAIKKIRDHVGKEAES"
     gene            814..1947
                     /locus_tag="PLP02_05970"
     CDS             814..1947
                     /locus_tag="PLP02_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43022.1"
                     /translation="MRKKDLYNMIRTKAMNDIPDILPKINLDNIEIIPAEEKAKKHFS
                     FAKLMSFSLTFAVIAIVAVFGINFLISEPTPNYLPLSSDAEIIGYQAVSATILTQEYY
                     LDELSYEILPLADSDEDLLISSLDTVDMYLGMFEYTLGDEDNVSFQYIESDQPQYQYC
                     IEFSGLDLMKNQTYLYFYYNASVANGSTEVDGIISTDDGEIAITGNIFANENAVRSTF
                     RAFRDDENYVEIQNRSTEQKQTFHYEIVENGVAGKKADVSVEAVKNRYRANIRMTEAN
                     GTLTMTVGKDKGNSKFAITYQASGTSDISGEIEVSVEYDETAGKYIYAYAVSQNGKVT
                     IYHGRRTETPASSIGPDNHRNTTIPGPQNSNYNPVESTDGYAV"
     gene            complement(1991..>2753)
                     /locus_tag="PLP02_05975"
     CDS             complement(1991..>2753)
                     /locus_tag="PLP02_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPF43023.1"
                     /translation="YYIRVEDVLVALEEKSIDLGWGISNYIATYLIMFIPTTFYYAKK
                     LRYGAFFIPVAAFEIVMLLFTVSRGGITAFAFVFILLVVYLFIKNKHWYSCFIAMIIT
                     AIAIMLLVYLNRSLFLASFDRFGRLLFDDSGRLDIYADAWAKFKDHPLFGNGIFARLD
                     TDGNFRMFHNTIFHVMATMGSVGLVGLIIQVVTMFLVILKKWRNESVILAIAIFGAHL
                     HGMVDNVYLMPQFMILLFLIIAVMENSNKANLKIE"
BASE COUNT          898 a          622 c          564 g          669 t
ORIGIN      
        1 ggcgttattc gttgttttca aggacaacgt caacgacatc aagaaaattc aagataccat
       61 cattcaattg atcgtcttct atttccgcga catcaacaat cgtttattct aaaccagctt
      121 aaccaaagcg gaggggcttc cctccgcttt ttttatttgt ttttgccatt tggaaaaaaa
      181 accaataatc tctatcactt gtccgtatat atgatgagat aaaaacagag tctttttcca
      241 aatgacgatg ttgtgatata atttattcga ggcggtgagg cttgtgacaa tcgacttcga
      301 caaatacatc gatcttctca aggaaaagga caacgaagcc tttgcctttg tttatgaaaa
      361 cacaaagcgg ggcgtcttct ctgtgattgc cccgattgtc agggacaaaa gcgaagtcga
      421 agatctgatg caggacacct acatcaagat gcttcaaaac atcaattcct atcaaaaggg
      481 acgcaatttc accgcatggt tgttccagat agccaagaat ctggcgattg attattacag
      541 aaaacaaaaa aggaccaccg tgcttgatcc tcaggatcat gaatacgtat ttgacaaaga
      601 acaggaaaca aacgaagatt atggtctgga aattaacgag gttatcggca ttctcagccc
      661 ggaagaaaaa gaaatagtgt tgctgaagat agtatcaggc aatgttttcc gagaaatcgc
      721 cgatatcgtc gacaaaccat taggaacggt tttatggatc tataacaaag caatcaagaa
      781 aatccgcgat catgtgggaa aggaggcaga atcatgagaa agaaagactt gtacaatatg
      841 ataagaacta aagcaatgaa tgacattccc gacattctgc cgaaaataaa tctcgacaac
      901 atcgagatca tcccggcgga agagaaagcc aaaaaacatt tcagtttcgc caaactcatg
      961 tctttctcgc ttacgtttgc cgttatcgcc atcgtagccg tctttggaat caatttcctc
     1021 atcagcgaac caactcccaa ctatctgccg ctttcgtccg atgcggaaat aatcggctat
     1081 caggcggtgt cggctacgat tttgactcag gaatattatc ttgacgaatt atcatacgaa
     1141 atcctgccgc tggccgattc tgacgaagat cttctgattt ccagtcttga cactgtcgac
     1201 atgtacttag gaatgttcga atacacgttg ggagacgaag ataacgtatc tttccaatat
     1261 atcgaatcgg atcagcccca gtatcaatat tgcatcgaat tttccggact ggatctaatg
     1321 aaaaatcaaa cataccttta cttctattat aatgcatccg tagccaacgg atccaccgag
     1381 gtcgatggca ttatctcaac agatgacgga gaaatcgcga tcactggcaa catcttcgcc
     1441 aatgaaaacg ccgtccgttc cactttccgg gctttccggg atgacgaaaa ctatgtcgaa
     1501 atccaaaacc gttcgaccga acaaaaacaa accttccatt acgaaatagt ggaaaatggc
     1561 gttgctggca aaaaagccga tgtcagcgtc gaagctgtca aaaatcgtta ccgggccaat
     1621 atccggatga cggaagcgaa cggaacactg actatgacgg tcggaaaaga caaaggcaat
     1681 tccaaattcg ccataaccta tcaggcaagc ggaacctccg atatttcggg agaaatcgaa
     1741 gtgtcggtcg agtatgacga aaccgcggga aaatatatct acgcttatgc tgtaagtcaa
     1801 aacggcaaag taaccatcta tcacggcaga aggaccgaaa caccggcttc ttccatcgga
     1861 ccggacaacc atcgcaatac aacgattccg gggccgcaaa acagcaatta taatccagtt
     1921 gaatcaacag acggatatgc cgtctgacaa aaagatccgg aaacccggat cttttttata
     1981 tttggacttg ttattctatt ttgagattcg ctttgttgga attttccatc acggcaatga
     2041 tcagaaacaa aaggatcatg aactgaggca tcagataaac attgtcgacc attccgtgca
     2101 agtgggctcc gaagatcgca attgccaata taaccgattc attccgccac tttttgagaa
     2161 taactaaaaa catcgtcaca acctgaatga tgagaccgac caatcctacg gaacccatcg
     2221 tcgccatcac gtggaaaatt gtattgtgga acatccggaa attgccgtct gtgtctagtc
     2281 tagcgaaaat accgtttccg aacaagggat gatccttgaa tttcgcccag gcatcggcat
     2341 agatgtccag cctcccggaa tcatcgaaca ataaccttcc aaagcggtcg aaagatgcca
     2401 ggaaaagcga acggttcaga tagaccaaaa gcatgatcgc tatcgctgtg ataatcatgg
     2461 caatgaagca ggaataccaa tgtttgttct ttataaaaag atatacaacc aaaaggatga
     2521 acacgaaggc aaaagcagtg atacctccac gcgataccgt gaataaaagc atgacgattt
     2581 caaaagcggc gacaggaatg aaaaaagctc cataacgcaa tttcttcgca taataaaatg
     2641 ttgtcggaat gaacatgatc agataagtgg ctatgtaatt ggatattccc cacccgagat
     2701 caatcgactt ttcctccaaa gccaccagca cgtcttctac cctgatatag taa
//
LOCUS       DAOPJL010000067         2638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_44582_length_2638_cov_5.429346, whole genome shotgun sequence.
ACCESSION   DAOPJL010000067 DAOPJL010000000
VERSION     DAOPJL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44582_length_2638_cov_5.429346"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLP02_05980"
     CDS             <1..512
                     /locus_tag="PLP02_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF43024.1"
                     /translation="EVDGVDYKTIKTASLNKEVSYIYQDVFLFEASLYDNITLFKPIP
                     KEKVEKVIELSGLSEFTKSLEDGLDTQIMENGKNLSGGERQRISIARALAKNADLLFI
                     DEATASLNEELGRAIEETILDLKATVVAISHRYYEGISENYDYVLELIDGYITQYPAR
                     EYFQGVNAA"
     gene            509..2143
                     /locus_tag="PLP02_05985"
     CDS             509..2143
                     /locus_tag="PLP02_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF43025.1"
                     /translation="MKKRWRYFSGLFYVIPPWIVYAFLNIKMSLKMLDVMNAAIDNDW
                     TAFWNNTIWLFVFLALVYPMDILSSYASITFTKKTVTKMKSDYIAAVFGKDIAEFQKE
                     NNAAYLSAVTNDFAIIETDYISQIMEIVVNLIRFASAIIIFSIISPLILLVGIGIVLI
                     NGSLTIFIEKPVKKHNTERSVLYAEYNSFVKEVLSAFSIIKNNNLETRIRKNFYDKSE
                     KVQQKRYIIDKLMSFVFSAQNAIGMSIFIGMSLFAVWMTMIGTVTFAGIIIIINNFGN
                     LVNPMFSVAEAIPKFRSVRAIFIRIDKTLEQKNNHEETLPFNKFEHEITFKNVAFAYE
                     DNPVVSHVDLTFEKGRKYLVIGPSGGGKSTLLRMLRKYFDPQEGIIEIDGNNLSDILK
                     KEYFSKIANIEQTIFLFEDTIRNNLTLFKDYSEEEIGDAVKKAGLDTFIDSNPEGLDY
                     MIYDNGKNISGGEKSRIAIARGLLNKAQIIFLDEAFASLDYDRAKKIEKTLLALDGVT
                     VINVSHVVIPENKLAYDGICVVKNHTASMRLQNQSE"
     gene            complement(2209..>2638)
                     /locus_tag="PLP02_05990"
     CDS             complement(2209..>2638)
                     /locus_tag="PLP02_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43026.1"
                     /translation="PLTVANMWPINKSYYSGLGKIYAGMRATLVGAIATNKKENEYFF
                     KKDIIFSAKSFANMGDDLPIKYSGGELFVMIMKIVWGIMTFKLRFKTIKSLLNGMKSG
                     DKISALYDRYPETTEGFAVWKEEADKLWKSSGSIADVKYD"
BASE COUNT          788 a          564 c          573 g          713 t
ORIGIN      
        1 tcgaagtcga tggcgtcgac tacaaaacga tcaaaacggc ttcccttaac aaagaggtat
       61 cttatatcta tcaggatgtc tttctgttcg aagcatcgtt gtatgacaat ataaccttgt
      121 ttaaaccgat tccgaaagaa aaggtcgaga aagtgattga actttccggt ttgtctgagt
      181 tcacaaaatc cttggaagac ggcttggata ctcagataat ggaaaatgga aagaatcttt
      241 ccggaggcga acgtcaaagg atttccattg ccagagcttt ggccaaaaat gccgatttgc
      301 tgtttatcga cgaagctacc gcatcattga acgaagaact cggaagggca atagaagaga
      361 ctattcttga tctgaaggcg acggttgtcg ccatcagtca ccgctattat gaaggaatca
      421 gcgaaaacta cgattacgtg cttgaactta tcgacgggta cataacccag tatccggcaa
      481 gagaatactt ccagggggtg aatgccgcat gaaaaagaga tggcgatatt tttccggatt
      541 attttatgta attccgccct ggattgtcta tgctttcctg aatatcaaaa tgagcctgaa
      601 aatgctcgat gtgatgaatg ccgccataga taacgactgg acggctttct ggaacaacac
      661 gatttggctt ttcgttttcc tggctttggt atatccgatg gatatcctgt caagttatgc
      721 ttcgatcact ttcaccaaaa aaaccgttac aaaaatgaaa tcggattaca tcgcagcggt
      781 tttcggcaag gatattgccg aattccaaaa agagaacaat gcggcttatc tgtccgctgt
      841 caccaatgat tttgccatta tcgaaaccga ttatatctcg caaatcatgg aaatcgtcgt
      901 taatctgatc agatttgcgt cggccatcat tattttttcc atcatcagtc cgcttatctt
      961 actggtcggt atcggcattg tcttaatcaa cggaagcctg acaatcttca tcgaaaagcc
     1021 ggtgaagaaa cataataccg aaagaagcgt cctgtatgca gaatacaact cgttcgtcaa
     1081 ggaagttctt tccgctttca gcattatcaa aaacaacaat ttggaaacca gaatcaggaa
     1141 gaatttctat gataagagcg aaaaggtcca gcaaaagcgt tatatcatcg acaagttgat
     1201 gagttttgtc ttctccgccc aaaatgcgat aggtatgtcg atcttcatcg gaatgtccct
     1261 gttcgcggta tggatgacga tgatcggaac cgtgacattt gccggaatca tcattataat
     1321 caataacttc ggcaatctgg tgaacccgat gttctcggtt gccgaggcaa ttcccaaatt
     1381 ccgttccgta agggccattt tcatccggat agataaaaca ctggaacaga aaaacaatca
     1441 tgaagaaacc ttgcctttca acaaattcga acatgaaatc acttttaaaa acgtggcatt
     1501 cgcatacgag gataatccgg ttgtaagcca tgtcgatctg acattcgaaa aaggcaggaa
     1561 ataccttgtg atcggtccgt ccggaggcgg gaaatcaacg ctattgagaa tgctcaggaa
     1621 atatttcgat ccgcaagaag gcatcatcga aatcgacggc aataatctgt cggatattct
     1681 aaagaaggaa tatttctcca aaattgccaa tatcgaacag acaattttcc tatttgaaga
     1741 tacgatcaga aacaacttga ctcttttcaa agattactct gaggaagaaa tcggcgatgc
     1801 cgtcaaaaaa gccgggctcg acacattcat tgacagcaat cccgagggat tagattacat
     1861 gatctacgac aacggaaaga acatatccgg tggcgaaaaa agccggattg cgatcgccag
     1921 aggacttcta aacaaagcac agatcatttt cctggatgag gctttcgcca gtctggacta
     1981 cgacagggcg aagaagatcg agaagaccct gcttgccctt gatggcgtaa cagtcatcaa
     2041 cgtaagccat gtggtgatac cggaaaacaa acttgcttat gacggtattt gtgttgtcaa
     2101 gaatcatacc gcctcgatgc gtcttcaaaa ccaatccgaa tgacaaactt catataataa
     2161 gaaaacaggg atccgtttta cgggtccctg tttgtttcgt tatgagattc aatcgtattt
     2221 cacgtcagct atgctaccag aagatttcca taacttgtcg gcttcttcct tccatacggc
     2281 aaaaccttcc gtagtctctg ggtatctgtc atataaagcg ctgatcttgt cgccggactt
     2341 catgccgtta agaagggact tgatcgtttt aaaccgaagt ttgaaagtca tgatgcccca
     2401 gacgattttc atgatcatta cgaaaagttc gcctccggaa tacttgatcg gtaagtcatc
     2461 tcccatgttc gcaaacgatt tggcggagaa gatgatatcc tttttgaaga agtattcgtt
     2521 ttcctttttg ttggtcgcta tggctccgac aagtgtagct ctcatgccgg catatatttt
     2581 tcccaggccg gagtaatagg atttgttgat gggccacata ttggcaacag taagcggt
//
LOCUS       DAOPJL010000068         2593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_45768_length_2593_cov_9.015366, whole genome shotgun sequence.
ACCESSION   DAOPJL010000068 DAOPJL010000000
VERSION     DAOPJL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45768_length_2593_cov_9.015366"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1270)
                     /gene="pepT"
                     /locus_tag="PLP02_05995"
     CDS             complement(56..1270)
                     /gene="pepT"
                     /locus_tag="PLP02_05995"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPF43027.1"
                     /translation="MKAYERLLKYVKIDTESDPVSEDYPSTPGQVEFMKMLAGELASM
                     EIESRLDEYGYLIAKIKANKPDVPKIALIAHVDTSPDASGKNVNPRIIKHYDGGKIGL
                     NKDTSMILDPEVFPSLKHAIGQDLITTDGTTLLGADDKAGVAEVMTVAEYLTTHPETK
                     HGEIILVFTPDEEVGNGTKYIDVAKIGADFAYTLDGSQAGEISFENFNAAAATVTITG
                     RSVHPGEAKGKMLNSISVAAEFDDLLPKYSRPETTEKYEGFNHLHLITGNAGKTVMEY
                     IIRNHDKRIFENQKTDFKNASAFLNTKYGPYTCEVEIVNSYFNMNEKLKDHPEVINLA
                     IEAIKECNIEPIIEPIRGGTDGARLTYLGLPCPNLGTGGYNYHGPYEYASIQEMDLAV
                     DIVLKIIAISTK"
     gene            complement(1275..1952)
                     /locus_tag="PLP02_06000"
     CDS             complement(1275..1952)
                     /locus_tag="PLP02_06000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF43028.1"
                     /translation="MKILYQDKKILIVDKPAGMLSHPAKGHREPDLLSELRHPEYSLI
                     SRLDYQTSGLLLVAKDAESASALNRLATKNFIKKIYMAVLSGYLSNKEDVLHSWLLKD
                     ELSAIVRLSDHEIPGSVRIVTRYRVLKQSNGFTLAEIELETGRTHQIRAQMAKIGHPV
                     VGDPLYGDKNLNKRNGLFSQALVSMEIRFAVDDEKDPFRYLDDKTFTKDEFPFIYLME
                     QNRPRKA"
     gene            complement(1985..>2593)
                     /locus_tag="PLP02_06005"
     CDS             complement(1985..>2593)
                     /locus_tag="PLP02_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPF43029.1"
                     /translation="TLIIKLFQYGDTTWLQAIFQSITTRTAGFSTINISTFSYTTLII
                     IMMFMFIGASPNSTGGGIKTTTFYVIWRSIISFLSGKPTIVKGRKIDDDTRAKAFMLM
                     ALASTAIIVVFIAISQIEMHNTTYDMTLLNLLFETVSAFGTVGLSMGVTPYLLPASKI
                     ILSLLMFTGRLGPITIFGILNRNWGHPYASSVEYPSEKILVG"
BASE COUNT          621 a          545 c          658 g          769 t
ORIGIN      
        1 tttggaatta ggatcagttc ataaaagaaa agcatccttc tggatgcttc tttttttatt
       61 tagtcgatat ggcaatgatt ttgagaacga tatccacagc taggtccatt tcctggattg
      121 aggcgtattc gtaaggaccg tgatagttgt aaccgcccgt tccgagattc ggacacggaa
      181 gacctaggta agtcagtctg gctccgtcgg ttcctcctcg aattggttcg atgatcggct
      241 caatattgca ttccttgatg gcttcgatcg ccaaattgat gacttccgga tgatctttca
      301 atttctcatt catgttgaaa taagaattca cgatttcaac ctcgcatgtg tacgggccgt
      361 atttggtgtt taagaaagcg gaggcatttt tgaaatcggt tttctgattt tcaaatattc
      421 tcttgtcgtg attgcggatg atatactcca tgacggtttt tccagcattt ccggtaatta
      481 gatggagatg gttgaaacct tcgtattttt cagttgtctc cggacgggaa tacttcggta
      541 gcaaatcgtc gaattcggct gccacgctga tggagttgag catttttcct ttggcttctc
      601 caggatggac agatcgaccg gtgattgtca ctgtggcggc agcggcattg aaattctcaa
      661 aggatatttc tcctgcctgt gatccgtcca gggtgtaggc gaaatcggca ccgattttgg
      721 ccacatcgat gtattttgtt ccgttgccga cttcttcgtc gggtgtgaag accagaatga
      781 tttcgccgtg tttcgtttca ggatgtgttg tgagatattc cgccaccgtc atgacttccg
      841 ccacgccggc cttatcgtcg gctccaagaa gtgtcgtgcc atcggttgtg atcaaatcct
      901 gcccgatagc atgtttcaag gaaggaaata cttctggatc caagatcatg gaagtatcct
      961 tgttcaatcc gatttttcct ccgtcataat gcttgataat tcggggattg acattttttc
     1021 cactggcatc gggcgaagtg tcaacatgag cgatcaaggc gattttcggt acgtcaggtt
     1081 tattagcctt gatcttggcg atcaggtatc cgtattcgtc gagtcgggat tcgatttcca
     1141 tgctggcgag ctcgccagcc agcatcttca tgaattcgac ttgtccggga gtcgacgggt
     1201 aatcttcgga cacaggatcg gattcggtat cgattttcac atacttcaac aagcgttcgt
     1261 aagctttcat ataatcacgc ctttcttggt ctgttttgtt ccatgagata gatgaaggga
     1321 aattcatcct ttgtaaatgt cttgtcgtcg agataacgga agggatcttt ttcgtcgtcg
     1381 acagcgaacc ggatttccat cgaaaccagc gcctggctaa acagtccgtt gcgtttgttc
     1441 agatttttgt cgccatataa tgggtcgccg acgacgggat ggccgatttt ggccatctgg
     1501 gcgcggattt gatgggttcg tccggtttcc agttcgattt cggccagtgt aaacccgttg
     1561 ctttgtttca gtactctgta tcgggtgacg atccggacgg atccggggat ctcatggtcg
     1621 gaaagcctga caatggcgct taattcatct tttaaaagcc atgagtgaag gacgtcttcc
     1681 ttgttggaaa gatatcccga caagacggcc atataaatct ttttgatgaa gtttttggtg
     1741 gccagacgat tcaaagccga ggcagactcc gcatctttgg cgacaagcaa aagcccggat
     1801 gtctgatagt ccaacctgga tataagggaa tattcgggat gacgtaattc gcttaaaaga
     1861 tccggttccc gatgcccttt ggcaggatgg gaaagcattc cggccggttt atcgacgatt
     1921 aatatttttt tatcctgata taatattttc ataattttat tgtctcaaaa gctcattgat
     1981 tttctcatcc gacaagaatc ttttcggaag gatattcgac gcttgaggca tatggatgac
     2041 cccaattccg gttgagaatg ccgaaaatgg tgatcggtcc caagcgaccg gtgaacatca
     2101 gaagcgagag gatgattttg gatgccggca acaaataagg cgtgacgccc atggaaagac
     2161 cgacggtgcc gaaagccgaa acggtttcga ataacagatt gagcaatgtc atatcgtatg
     2221 tggtattgtg catttcaatt tgggaaatgg cgatgaaaac gacaatgatg gcagtggatg
     2281 ccaaagccat caacatgaag gctttggctc tggtgtcatc gtctattttc cgtcctttga
     2341 cgattgtcgg tttaccgctc agaaaactga tgatcgaacg ccatatgaca tagaaggtgg
     2401 tggttttgat accaccgccg gtggagttcg gcgaagcgcc gatgaacatg aacatcatga
     2461 tgatgatcag ggtcgtataa gaaaaggtgc taatgttgat ggttgagaaa ccggcagtcc
     2521 tggtcgtgat actttggaaa atcgcctgta accaggtggt atcgccatat tggaacagtt
     2581 tgatgatcaa ggt
//
LOCUS       DAOPJL010000069         2569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_46345_length_2569_cov_7.452267, whole genome shotgun sequence.
ACCESSION   DAOPJL010000069 DAOPJL010000000
VERSION     DAOPJL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46345_length_2569_cov_7.452267"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..2021)
                     /locus_tag="PLP02_06010"
     CDS             complement(45..2021)
                     /locus_tag="PLP02_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF43030.1"
                     /translation="MIKKIGLVMSLLLVTFLFVGCRPNITTADLLSQEPTVADGYLVD
                     NVQDGLILHAWNWSMSLIEEHLEDIAIAGFSTVQISPMQPQKDYFGVASWGSSWWKLY
                     QPLGFSVATENHSLGTKDDLESLCTAADAYGIKIIVDVVANHLAGGDNITLNENIAGY
                     EPEIYNQNLIHQNSGYVSDSSIYAVTKLPLGGYPDLMTESTVVQERVLSLLKEYVDAG
                     VDGFRFDAAKHIETPEDGEYASNFWLYVIDGINDYAETLGIDNLYFYGEILNTVGADR
                     SFTAYTEYMSVTASSLSDTIRHAIVNRNANTLQTASYITDVSANKTVLWAESHDLYAG
                     GTTLDTSDETITKAYAIEASRKDATTLYFARPSDTSFMGEIGSYLWQSVEVSSVNKFH
                     NYFVGSEEEITIEDGFFLNERYNDTASGVVIVDVERNGDISGVAVSHMADGYYMDQVS
                     GEYFRVKNGEISGSISDSGIAVVYNNVYEPKPAVYVSDDGIHGSFSDTKTISVYSYNT
                     TEAYYSLNGDDKIAFSGNIDLELSSPDLNVIVTLDFELWYGDYKIERHYEYVKSNIVV
                     TEVVVSNIDDGAVAGNKIVAWTWSQGEEGKWMEGTYVNGTFTFDIETGDDYFLLVTFP
                     SGTDTFNWEIKIAQTNDVEIPSDGLYDGSQLIWN"
     gene            complement(2177..>2569)
                     /locus_tag="PLP02_06015"
     CDS             complement(2177..>2569)
                     /locus_tag="PLP02_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43031.1"
                     /translation="VSPESTSFILLVGVSWELSMMFALAGLIMSKLLPADPKMKILGI
                     NNRVFLAILNAAFFSLFEIFLAGTPAFIWTYSWWGAVPVFVTTYIPFFLAAFLVYDAK
                     PKTQKRFLAVVWGLAALLLIVLILLGII"
BASE COUNT          680 a          628 c          555 g          706 t
ORIGIN      
        1 aaaaagaagc atccagaagg atgcttttct tttatgaact gatcctaatt ccaaatcaac
       61 tggcttccgt catatagtcc gtcactggga atttccacat cattggtctg ggcgatcttt
      121 atttcccaat tgaaggtatc tgtcccggac gggaaagtca ccaaaaggaa gtagtcgtca
      181 ccagtttcta tatcaaatgt gaaggtgccg tttacatatg ttccttccat ccatttgcct
      241 tcttcgccct ggctccatgt ccaagctacg atcttgttgc cggcaacggc tccatcgtcg
      301 atattgctga caacgacttc ggtaacaacg atattgctct tgacatactc ataatgcctt
      361 tcgatcttgt aatcgccata ccacaattca aaatcgagcg tgacgataac gttcaaatcc
      421 ggactcgaaa gttccaaatc gatatttccg gaaaaagcta tcttgtcgtc tccgttcagg
      481 gaataataag cttcggttgt attatacgaa tacaccgaaa tggtctttgt atcggaaaat
      541 gatccatgta ttccgtcatc agagacatat actgccggtt tcggttcgta aacattattg
      601 tagacaacgg caatgccgct atcgcttata ctgccggata tctctccgtt tttgacccgg
      661 aaatattcac cagacacctg atccatataa tagccgtccg ccatatggga aacagcaact
      721 ccggagatat ctccgtttct ttcgacatcc actatcacga ctccggaagc cgtgtcgttg
      781 tatctttcat tcaaaaagaa accgtcttcg atggtaatct cttcttcgga tcccacaaaa
      841 taattatgga atttgttcac ggaggacact tcgacggatt gccacaaata tgatccgatc
      901 tcacccatga aagacgtatc cgaaggtctg gcaaaatata aggtcgtcgc atcttttctc
      961 gaagcctcga tggcataagc tttggttata gtctcatcgc tagtatctaa ggtagttcct
     1021 ccggcataaa gatcatgcga ttcagcccat aaaacagtct tattggctga aacatcagtt
     1081 atgtatgagg ccgtctgaag cgtgttggca ttcctgttca cgatcgcatg tctgatcgta
     1141 tccgaaagac tgctcgcagt gacagacata tattcggtat aagccgtgaa cgaccggtcg
     1201 gctccgaccg tattaaggat ttctccgtaa aaatatagat tgtctatccc gagagtttcc
     1261 gcgtaatcgt ttatcccgtc aatcacatac aaccagaaat tgctggcata ttcaccgtct
     1321 tccggtgttt cgatgtgttt tgccgcatcg aatctgaatc cgtcgactcc cgcatccaca
     1381 tactctttta ataggctcaa tactctttcc tggactacgg tgctttcggt catcagatcc
     1441 ggataacctc ccaatggcag tttggtcacg gcatagatgc tgctgtcgct cacatatccg
     1501 gagttctgat ggatcaggtt ctggttatag atttccggtt cgtatccggc gatattctcg
     1561 ttaagcgtta tgttgtcgcc gccggccaaa tgattggcga cgacgtcgac gatgatcttg
     1621 attccgtagg cgtcagccgc cgtgcaaagc gattcaagat cgtccttggt gccaagcgaa
     1681 tgattctccg ttgcgacgga aaaaccgagc ggctgataaa gcttccacca actagatccc
     1741 cagcttgcca ctccgaaata atccttctgc ggctgcattg gcgatatctg aaccgtactg
     1801 aatccggcga tggcgatatc ttccagatgc tcttcgatta aagacatcga ccagttccag
     1861 gcgtggagga tcaatccatc ctggacatta tcgaccagat atccgtctgc aacggtcggt
     1921 tcctggctta acaggtcggc cgtcgtgata ttcgggcggc atccgacaaa cagaaatgtt
     1981 accaacagga ggctcatgac gagcccgatt tttttaatca tggcattctc cttgtgatat
     2041 ttctcgtgtc tttattttat cacacgaaag acaatttatg attgctttca tttcatgttt
     2101 tatgatttat ccctgttttt aaaaaaagaa aagatgccgg tttttctcat gccggcatct
     2161 ttataaacag aatttgtcag attattccaa gcaagatcaa cacaatcaat aacagggccg
     2221 caagtcccca gacaaccgcc agaaatcttt tctgggtttt cggcttggcg tcatagacaa
     2281 gaaaagccgc caggaagaaa ggtatgtagg tggtaacgaa caccggaacg gctccccacc
     2341 aggaatatgt ccagatgaaa gccggcgtgc cggcgaggaa aatctcgaaa agactgaaga
     2401 aggctgcgtt caggatcgct aggaaaaccc ggttgttgat accgagtatc ttcattttcg
     2461 gatcggccgg caacaattta gacatgatga gtccggcaag agcgaacatc atggataatt
     2521 cccaggacac tccgacaagt aaaatgaagg aggtgctttc cggagaaac
//
LOCUS       DAOPJL010000070         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_48049_length_2503_cov_8.905637, whole genome shotgun sequence.
ACCESSION   DAOPJL010000070 DAOPJL010000000
VERSION     DAOPJL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48049_length_2503_cov_8.905637"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..651
                     /locus_tag="PLP02_06020"
     CDS             109..651
                     /locus_tag="PLP02_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPF43032.1"
                     /translation="MFITIFENYLDTGPGIGEMLLRMGLTAVFVGIIGIERQMRHKIA
                     GVTTHLMIAFAACAIAILQDALYLESLSQAEALAAQGVSVSLERQRIIAQVVTGVGFL
                     GAGTILKTSNGIYGLTTAATLFLAAMIGICFGMGGYVIGIILSMFAFLFLTGFRKLLD
                     RTAIKHTKSPEILGSEVIEK"
     gene            799..1632
                     /gene="sufC"
                     /locus_tag="PLP02_06025"
     CDS             799..1632
                     /gene="sufC"
                     /locus_tag="PLP02_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPF43033.1"
                     /translation="MAKLEIKNLVASVEGKIILNGVDLVVSGGMTHAIMGPNGTGKST
                     LASVIMGHPKYKIESGDILLDGESILDMPTDERSRRGLFLAMQNPVEVPGVTNFDFIR
                     TAVSARLPKGEHLRVGKFIFRFEDAAKALKMGSGLSERFLNQGFSGGEKKRNEILQMS
                     LLSPKIAVLDEIDSGLDVDALKLVGENVTAMKSPELGLLMITHYQRLLDYIVPDVVHI
                     MIGGRLVKSGGKELIARIDSEGYDWLREELGIDELTEKPERYSLGSCANNLKLNEQVK
                     K"
     gene            1629..>2503
                     /locus_tag="PLP02_06030"
     CDS             1629..>2503
                     /locus_tag="PLP02_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPF43034.1"
                     /translation="MMPYPNYLKSLEEAFFPEINNKSVLKSAVFSDGKLSFGDDSDSM
                     ATVVLTDGTSDFAIETDKGKKTALHLVVWSDKETDKNISIVLKENSRLDLVVSLLGKG
                     TIRINQAHAFEENSVLESRIGLVRSGKTEYTQKYLLDGRNASVNQILFAVSSGNDRTS
                     ITEKVYHNKPDTKSDLNNNLVANGKANIYLEVTGKIEKGMHGSECLQQNRGILLSETA
                     EIEVDPELLIDEFDVNARHGAAIGQINEEELFYLLSRGITNEEARKLIISGYMGPYLE
                     KLSDTPFGSRIANRI"
BASE COUNT          707 a          587 c          630 g          579 t
ORIGIN      
        1 caaaaaaccg gaaattgacc ttgaattttc catatatgtc tgatataata ctaatttaca
       61 ataaagcgct tacacatttg gcctcgccaa cgaggaaggt gaagatatat gttcattacc
      121 atttttgaga attaccttga taccggcccg ggaatcgggg aaatgttgct acggatgggg
      181 cttaccgccg tgtttgtcgg catcatcggc atcgaaaggc agatgcgtca caagatcgcc
      241 ggcgtcacca cccatctgat gatcgccttc gccgcctgtg ccatcgccat cctgcaggac
      301 gcgctttatc tcgagtcatt gagccaggcg gaagccttgg ccgcccaggg cgttagcgtc
      361 agccttgaaa gacagagaat catcgcccag gtcgtcaccg gagtcggctt ccttggagcc
      421 gggacgattt tgaagacctc gaacggaatc tatggtctca ccaccgccgc caccctgttt
      481 ctggcagcca tgatcgggat ctgtttcggt atgggcggat atgtgatcgg gatcatcctg
      541 agcatgtttg ccttcctgtt tttgaccgga ttcagaaaac tgctcgaccg taccgccatc
      601 aagcatacca aaagtccgga aattctcgga tcggaagtaa tcgaaaaata agattttttg
      661 aagcgacaaa caacccggtt ctccaaaaaa gagccgggtt ttttcatgaa aacccggccg
      721 gaggcaggtt ttcattcata ttttttgcaa tacggttgta aatagggtaa aatatactat
      781 ggctttggag gtggcaaaat ggcaaaattg gaaattaaaa acctggtagc cagcgttgaa
      841 ggaaaaatca ttcttaacgg tgtcgatctg gtcgtttccg gtggaatgac ccatgcgata
      901 atgggtccga acggcaccgg aaaatcgacg cttgcgtccg ttatcatggg gcaccccaaa
      961 tataagatcg aatcgggaga catcctgctt gacggagaga gcattctaga catgccgacg
     1021 gacgaaagaa gccggcgcgg tctttttctc gccatgcaga acccggtgga agtgccggga
     1081 gttaccaatt tcgatttcat cagaaccgcg gtttctgcac gcttgcctaa gggcgaacat
     1141 ctcagggtcg gaaaattcat tttccgtttc gaggacgccg ccaaggctct caaaatgggt
     1201 tcgggattgt cagaacgttt cctgaatcag ggtttttccg gtggtgaaaa gaagcgtaac
     1261 gagattcttc aaatgtcgct tttgtcgcca aaaatagccg ttttggatga aatagactcc
     1321 gggcttgacg tcgatgcgct gaaattggtc ggcgaaaatg tcacggcgat gaaatcgccg
     1381 gaactcggat tgctcatgat aacgcattac caaagacttc tggattatat cgttccggat
     1441 gttgtccaca tcatgatcgg cggcagactg gtcaagagcg gcggcaagga actgatcgcc
     1501 cggatcgata gcgaaggata cgactggttg cgggaagaac tcggaatcga cgaactgacg
     1561 gaaaaaccgg aacggtattc cctcggatcc tgcgccaaca acctgaagtt aaacgaacag
     1621 gtgaaaaaat gatgccttat cccaactatc tcaaatcgct ggaagaagct tttttcccgg
     1681 agataaacaa taaaagcgtg ctgaagtcag ccgttttttc cgacgggaaa ctatcctttg
     1741 gcgatgactc cgatagtatg gctacggttg tattgacgga cgggacatcc gatttcgcca
     1801 tcgaaacgga caaaggtaag aaaaccgctt tgcatttggt tgtctggtcg gacaaagaaa
     1861 ccgacaagaa catcagtatt gttttaaaag agaattcccg tctggatctg gttgtttccc
     1921 tattgggaaa agggacaatc cgcatcaacc aagctcatgc gtttgaagaa aacagtgttc
     1981 tggaaagccg gataggactg gtaagatcag gaaaaaccga atacacccaa aaatatcttc
     2041 ttgacggcag aaacgcatct gtcaatcaga tacttttcgc cgtatcatcc ggaaacgacc
     2101 ggacaagcat cacggagaaa gtctatcata acaaacccga taccaaatcc gaccttaaca
     2161 acaatctggt ggccaacggg aaagcgaaca tttatctgga agtgactggc aaaatcgaaa
     2221 aagggatgca cgggtccgaa tgtcttcagc aaaatcgcgg aatccttcta agcgaaaccg
     2281 ccgagatcga agtcgatccg gaacttctga tcgacgaatt cgatgtcaac gcgagacacg
     2341 gagccgctat cggacagatc aacgaagaag aactttttta tcttttaagt cgcggcataa
     2401 cgaacgagga agcccgaaaa ctgatcattt ccggatatat gggcccttat ctggaaaaac
     2461 tcagcgacac gccgtttgga agccgcattg ccaaccggat cga
//
LOCUS       DAOPJL010000071         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_55816_length_2256_cov_5.288960, whole genome shotgun sequence.
ACCESSION   DAOPJL010000071 DAOPJL010000000
VERSION     DAOPJL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55816_length_2256_cov_5.288960"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLP02_06035"
     CDS             <1..257
                     /locus_tag="PLP02_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43035.1"
                     /translation="KYMWVFLAFMVPMLLVVINVIPEDYDFLALISPSYGAQILYQSA
                     VGIATKNIQVIFSAIYLFLLGFLLYPLVVLPKFAKSALEG"
     gene            265..1071
                     /locus_tag="PLP02_06040"
     CDS             265..1071
                     /locus_tag="PLP02_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43036.1"
                     /translation="MKKYLGLLKYEIKSFFKDALSKFFLLYPLLMLFICGFLLPEILD
                     RTASGNENAQTITLLIAFVVIISMGGYVMGVLLGFSLLDNKDENTLISIAASPVTVSG
                     YANFKTCYSYVFAIIANMVMLGGLKLVASDLYVVEYAGVTIRLLDELSWGNIVLITLV
                     GSLMVPTVALIVASIAKNKIEGFAFMKSGGIFVMLPLLALLDTFRDWKQYLLGIMPNF
                     WFTKPLLNEILPVSDPSDLPFWAYLAIGTVYMLFLAIITHRMFMKRNESN"
     gene            1084..1215
                     /locus_tag="PLP02_06045"
     CDS             1084..1215
                     /locus_tag="PLP02_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43037.1"
                     /translation="MDWYWYVIIGVGIILIGYLKWAVWNKMKANRQKKTENKEKDED"
     gene            complement(1328..>2256)
                     /gene="rnhC"
                     /locus_tag="PLP02_06050"
     CDS             complement(1328..>2256)
                     /gene="rnhC"
                     /locus_tag="PLP02_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPF43038.1"
                     /translation="NQVFSVSLDQLPALVDYYADFSVPSDKDHILYLFQSPDLTVTVF
                     RSLKVMLQGKDAWDEYMMWAEIFNFPLEEKKPEINPLPKVTSASSMTADNKRTIGSDE
                     VGTGDFYGPVVVAAALAGPDDISFLKSLGVRDSKQMSDENIREIGIKLIKALPSVILT
                     VDNEKYNNLVREGYNLNKIKAYLHNHAIRKLTAKCQGEFDQVVIDEFCPKEAYFSYLE
                     GQPVYRDILFRQKAESAYIAVAAASVLARYTFLTEMDKLNKEVGLILPFGASAAVDLL
                     GKRIALENGFDIFQKIAKTNFKNMDKIRNMMK"
BASE COUNT          588 a          499 c          517 g          652 t
ORIGIN      
        1 tgaaatacat gtgggttttt ctggcgttca tggtccccat gcttttggtg gtgataaatg
       61 tcataccgga agattatgat ttcttggctc tgataagccc gtcatatggc gctcaaatcc
      121 tttatcaaag cgcagtgggc attgctacaa agaacattca ggtgattttc tcggcaattt
      181 atcttttcct gctgggattc ttgctttatc cgctggtagt cttgcctaaa ttcgccaaat
      241 ccgcattgga ggggtgaaag gaagatgaaa aaatatctcg gattgttgaa atacgaaata
      301 aagtcatttt tcaaagacgc gttgagcaaa tttttccttt tgtatccttt gctcatgttg
      361 ttcatttgcg gctttctatt gccggagata ttggaccgga cggcctccgg taatgaaaac
      421 gcccagacaa tcaccttgtt gatcgccttt gtcgtgatca tctcgatggg cggttatgtc
      481 atgggagtgc ttctcggttt ttccttgctt gacaacaaag acgagaacac tttgataagc
      541 atcgctgcat cccctgtcac cgtttccggc tatgctaatt tcaagacgtg ttattcctat
      601 gtattcgcaa tcattgcgaa catggtcatg ctgggcggtc ttaaactggt agcttccgat
      661 ctttacgtag tcgaatatgc cggagtgacg attcgtctgt tagacgagct ttcctgggga
      721 aacattgtcc tgatcactct ggtcggaagt ctgatggtgc ctacagtcgc cttgattgtg
      781 gcttcgatcg ccaaaaacaa gatcgagggc tttgctttca tgaaaagcgg cggtatcttt
      841 gtcatgttgc cgttgcttgc gcttttggac actttccgcg attggaagca gtaccttttg
      901 ggaataatgc cgaatttttg gttcacaaaa ccgttattaa atgaaatact gccggtatcc
      961 gacccgtccg atttgccatt ttgggcttat ctcgcaatcg ggaccgtcta tatgcttttc
     1021 ctggcaatca tcacgcaccg tatgttcatg aaaagaaatg aatccaacta aggaggtaaa
     1081 gtcatggatt ggtattggta tgtcataatc ggagttggca tcatcctgat cggttatctc
     1141 aagtgggcgg tttggaacaa aatgaaagcc aacagacaga aaaaaaccga aaacaaagaa
     1201 aaagacgaag attaagaaat acggtccccg ggacgatggt tacctttaaa cccccgggga
     1261 ccgtgtttta tatataaaaa aagccattac cgacaaatgc ccggaaatgg cgattatcca
     1321 agaatattta cttcatcatg ttacgaattt tatccatatt tttgaaattg gttttagcga
     1381 tcttttggaa tatatcaaaa ccgttctcaa gcgcgatccg ctttccgagc agatcgacgg
     1441 cagccgaagc tccaaaaggt aagatcaaac cgacctcctt gttcaacttg tccatttccg
     1501 ttaagaatgt gtatctggcc aaaaccgatg ccgccgccac ggcgatatat gccgattcgg
     1561 ctttttgtct gaataggata tcccggtaga cgggttggcc ttcgagatag gagaaatatg
     1621 cttctttcgg gcagaattcg tcgatcacga cctgatcgaa ttctccctgg cactttgccg
     1681 tgagttttct gattgcgtgg ttatgcaaat aagccttgat cttgttcaga ttgtatcctt
     1741 ccctaactag attgttgtat ttttcattat cgaccgtcaa aatgacacta gggagcgcct
     1801 tgattaattt gatgccaatt tccctgatgt tttcgtcaga catctgcttg ctgtcgcgaa
     1861 ctcccaaaga cttaagaaag gaaatgtcat cggggccggc caaagcggcc gccacaacga
     1921 caggaccgta gaaatctccg gtgcctactt catccgagcc gatggtcctt ttgttatctg
     1981 cagtcatcga agaagcggat gtcaccttgg gcaaaggatt gatctccggt tttttctctt
     2041 ccaaaggaaa gttgaatatc tcagcccaca tcatgtattc gtcccaggcg tccttgccct
     2101 gcaacatgac tttcagagaa cgaaaaacag tgactgtgag atcgggggac tggaaaagat
     2161 acaaaatatg atccttgtcg gaaggaaccg aaaaatcggc ataataatcc acaagtgccg
     2221 gcaactgatc caacgagacg gagaaaacct ggttca
//
LOCUS       DAOPJL010000072         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_58762_length_2175_cov_4.204717, whole genome shotgun sequence.
ACCESSION   DAOPJL010000072 DAOPJL010000000
VERSION     DAOPJL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58762_length_2175_cov_4.204717"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLP02_06055"
     CDS             complement(<1..934)
                     /locus_tag="PLP02_06055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF43039.1"
                     /translation="MNDYDIIVVGMATTGSYFARKMAEKGYRVLVIDKLPEEKVGCKY
                     DIVHMLKSDFDRFGLPKPEKGDDFAFEFTGGITYSAFGNYPKEGYGTTVGMHLHNYTL
                     HLNRWAAEAGVSLSYETEFNGILWRKDKIAGVKIHRNGKAETLTASLVVDASGIPSVV
                     REKLPASSFVENFVIKPEEMFYVILHYVKYKNKEDFVKVSRGWLYYKTWEAPEADPEG
                     AILGIGAIRSYAEGEKIYKEFIGSIQLPEYENKYVEKGRTPFRRAPYSFVDENFLVLG
                     DAACVNKPYNGEGITASMIHCDIAVRVIDELLKAN"
     gene            complement(937..>2175)
                     /locus_tag="PLP02_06060"
     CDS             complement(937..>2175)
                     /locus_tag="PLP02_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF43040.1"
                     /translation="PDDYQGPYWRKERLKSLFPDESEGIDAYYDFYDNMTALMRIALE
                     KPSIAQKVRLIRTFAKVKKYKDMNAQELMDTFFKSPKIKALFTGILADVCVKPSEFMA
                     LGVPMFNIETAFEKRIPLCENGKKIRNGYVYVKGSVNKIATELARVIEAFNGKIYTNE
                     TVTKINVGPNGVTGVNLSGNKTAEADIIIASGGANEVFRDLLGENHLDNEYKNILSAF
                     KPMDSVFMVHLGVDTNPLEYQKTALCYYYGSYDIEGAIDRIRNGIYHEGKDGFLIYVP
                     SIANPEMAPDGHYAVTIYTVAPDTLKTGIWETLKNEYADKLIGLASERIPSLKDHINE
                     KVIVTPIDFRRLTHLHKSAFGGLVPLWGVKNPPHKTPVKNLYFVGQQSENMGGVGAVM
                     LGAEITANLALKENRKERRK"
BASE COUNT          502 a          521 c          489 g          663 t
ORIGIN      
        1 tgttcgcttt caagagttcg tctatcaccc ttaccgcgat atcgcagtgg atcatgctgg
       61 cggtgatgcc ttcgccgtta taaggcttgt taacacatgc ggcgtcgccc aaaacaagga
      121 aattttcgtc gacaaacgaa taaggtgcgc gtctgaacgg cgtccgccct ttttcgacgt
      181 acttattctc atactctggc agttggatcg atccgatgaa ttccttgtag atcttttcgc
      241 cttccgcata actgcgaatg gctccaatgc ctaagatagc tccttccggg tcggcttcgg
      301 gagcttccca ggtcttgtag taaagccaac cccggcttac tttgacaaag tcttctttgt
      361 tcttgtattt gacataatgc agaatcacat aaaacatttc ttccggtttg atgacgaaat
      421 tttcaacgaa agaagaggcg ggaagttttt cgcgcacaac cgaaggaatt ccggaagcgt
      481 cgacgacaag cgacgcagtc agggtttccg ccttcccgtt tcggtgaatt ttaacaccgg
      541 cgattttgtc cttgcgccat aaaatcccgt tgaattcggt ttcgtaagaa agactgacac
      601 cagcttcagc ggcccagcgg ttcaaatgca aagtatagtt gtgaagatgc atgccgacgg
      661 tagttccata tccttccttc ggataatttc caaaagcgga gtaagtgatc ccgcccgtga
      721 attcaaaggc aaagtcgtct cctttttccg gtttcggcaa accgaagcgg tcgaaatcgc
      781 ttttcagcat gtgaacaata tcgtatttac atccgacctt ttcttctggc aatttgtcga
      841 tcaccagtac cctatagccc ttttccgcca tctttcttgc aaaatagctt ccggtggtcg
      901 ccattccgac gacgatgata tcataatcgt tcataatcat ttccttcttt ctttccggtt
      961 ttcttttaat gccagattcg cagtgatttc agcgcctaac ataacagctc cgacaccacc
     1021 catgttctcg ctttgctgtc cgacaaaata aagatttttc accggggttt tgtgaggcgg
     1081 gttcttcacg ccccataatg gcaccaagcc tccgaaagct gatttgtgaa gatgggtcag
     1141 cctacgaaaa tcgattggcg tcactatgac cttctcgttg atgtggtctt tcagggaagg
     1201 tatcctttcc gatgccaaac caatcagttt gtcggcgtat tcattcttga gagtttccca
     1261 gattcctgtt ttcaaggtgt cgggagcgac agtgtagatt gttactgcat aatggccatc
     1321 cggggccatt tccgggttgg cgatcgaagg aacgtaaatc aggaatccat ctttgccttc
     1381 atgatagata ccatttctga tacgatcgat ggcgccttcg atatcatacg agccgtagta
     1441 gtagcaaaga gcggtctttt ggtattccag cggatttgtg tcgactccca aatggaccat
     1501 gaaaacggag tccatcggtt taaatgccga aagaatgttt ttgtattcgt tatcaagatg
     1561 attttctccc agaagatccc tgaatacttc attcgctccg ccggaggcga tgattatgtc
     1621 ggcctcggcc gttttgtttc ccgaaaggtt gactccggtt actccgtttg gtcctacatt
     1681 gattttcgtc acggtttcgt ttgtatagat cttgccattg aatgcttcga tgactctcgc
     1741 aagctctgtc gcgattttgt tgacgctacc tttgacatag acataaccgt tacggatttt
     1801 tttgccgttt tcgcaaagcg ggatccgttt ttcgaaagct gtttcgatat tgaacatcgg
     1861 aacgcccaaa gccatgaatt cgcttggttt gacgcagaca tcagcgagaa tgccggtaaa
     1921 aagcgccttt attttcgggc ttttgaagaa ggtgtccatc agttcctggg cattcatgtc
     1981 cttatatttt tttactttgg caaatgttct gattaatctg actttttgag cgattgaagg
     2041 tttttctaag gctatacgca ttaaggcggt catattgtca tagaaatcgt aatatgcatc
     2101 gatcccctcc gattcatccg ggaacaggga tttaagcctt tctttccgcc agtatggccc
     2161 ttggtaatcg tcggg
//
LOCUS       DAOPJL010000073         2001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_66196_length_2001_cov_5.727646, whole genome shotgun sequence.
ACCESSION   DAOPJL010000073 DAOPJL010000000
VERSION     DAOPJL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66196_length_2001_cov_5.727646"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLP02_06065"
     CDS             complement(<1..286)
                     /locus_tag="PLP02_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPF43041.1"
                     /translation="MLKAKYIWKLQVEGIVPDTEIFRNILTNRGIDDPDRFFSLGEES
                     VYDPYLLKDMKKAATRIWKALSASEKILIYGDYDCDGISSISVLVRALRKL"
     gene            complement(360..1178)
                     /gene="nadC"
                     /locus_tag="PLP02_06070"
     CDS             complement(360..1178)
                     /gene="nadC"
                     /locus_tag="PLP02_06070"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPF43042.1"
                     /translation="MDEIIKIIKEALHEDMPKGDVSTEALFGEEVTKARLIAKESGVI
                     SGLDVAKAVFHTVDLKTEFEAILKNGDMVSKGDVIATVSGKTKSLLKAERTALNFLQR
                     MSGIATLTASFVQKTAGTKTKIYDTRKTTPNLRILEKKAVVDGGGVNHRMNLSDMVML
                     KDNHIKAAGSITEAVETIRKRYGKMFQVEVEVENCAMFEEALMSKCDIIMLDNMSNEE
                     MAKCVNRNSGKKILEASGNMTLDRIASVASLGVDRISVGMLTHSAKALDISMKF"
     gene            complement(1182..>2001)
                     /locus_tag="PLP02_06075"
     CDS             complement(1182..>2001)
                     /locus_tag="PLP02_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF43043.1"
                     /translation="NIVTIFQMIHSRSAQPFHLNVTEVNDLVKLLFAGVYPADKFQYR
                     KIDNKKKTLISQEGSSLREQLETLIELTGEGKKHGNYEPLFLHLNFMVDFLNMEIYRY
                     LPGDVIAVLVYYLLSMQDGMISFNYLSFFNKLLLNKDEFKAALEKTRFGWSEGYSEIM
                     PLERFYLRIYHELYIDLQEYARDYDYEKNLEISKSDYIENTIDKLPEVFQKEDIRTRH
                     PFISDSTINRTLSRMQSENKIRPLGKGRSAKWVKLYKKETGKFKGKQLTFDLEE"
BASE COUNT          463 a          466 c          449 g          623 t
ORIGIN      
        1 ccaacttccg caaagcgcgc accagcacgc ttatagaact gattccgtca cagtcgtagt
       61 cgccgtatat aagaattttt tccgatgcgg acaaagcttt ccagatcctg gttgcggctt
      121 tcttcatatc tttcagaaga tatggatcat ataccgattc ctcacccagt gaaaagaaac
      181 ggtcaggatc gtctatgcct cggtttgtga gaatgttgcg gaatatttcg gtgtcgggaa
      241 cgatcccttc gacctgcaat ttccaaatgt attttgcttt aagcaacctt catcacccgt
      301 ttcacgagaa tatattataa cctatggaga ataatttgcc agcggtgagg ctaaaaaaat
      361 cagaatttca tggaaatatc cagcgctttt gccgaatgtg tcagcattcc cacgctgata
      421 cggtcgacgc caaggctggc gacggaggcg atccggtcaa gcgtcatgtt gccggaagct
      481 tcgagaatct tcttgccgct gttcctgttg acgcatttgg ccatttcctc gttcgacatg
      541 ttgtcaagca tgataatgtc gcatttggac atcagggctt cctcgaacat ggcgcagttc
      601 tccacttcga cttcaacctg gaacatcttc ccgtaccgtt tgcggattgt ttcgacggct
      661 tccgtgatcg aaccggccgc tttgatgtgg ttgtccttta acatcaccat gtcggacagg
      721 ttcatcctgt ggttgacccc gccgccatca acaacagcct ttttttccag gattctgaga
      781 ttgggagtcg tttttctcgt gtcatatatt tttgttttcg ttcccgctgt cttttgtacg
      841 aagctggcgg tcaaagtcgc gatcccggac atcctttgta gaaaatttag agcggttctt
      901 tccgccttga gaagcgattt ggtcttgccg gatacggtgg cgatcacatc gccttttgaa
      961 accatgtcgc catttttcaa aatagcctcg aattcagttt tcaaatcaac ggtatgaaat
     1021 acggctttag ccacatctag accggaaatg acgcccgatt ctttggcgat cagtctggct
     1081 tttgttactt cttcgccaaa cagcgcttcg gtcgagacat cacccttcgg catgtcctca
     1141 tgcagggctt ctttgatgat ttttattatt tcatccatga cttattcctc cagatcgaat
     1201 gtaagttgct tgcctttgaa tttgccggtt tctttcttgt agagtttcac ccacttggcg
     1261 cttcttcctt tgcctaacgg tcttattttg ttttctgatt gcattctcga caaagtccgg
     1321 ttgattgtcg agtcggaaat gaagggatgg cgggtgcgga tgtcttcttt ttggaagact
     1381 tccggcaatt tgtcgatcgt gttttcgata taatcggatt tggatatttc caggttcttc
     1441 tcgtaatcgt aatccctggc atactcctgc aaatcgatat acagttcgtg ataaattcgc
     1501 agataaaaac gctcgagggg catgatctcc gaataaccct cgctccatcc gaagcgggtt
     1561 ttttccagcg cagctttaaa ttcatccttg ttcaaaagaa gtttgttgaa gaaggaaaga
     1621 taattgaatg atatcatgcc gtcctgcatt gacaacaagt aatatacgag gacggcgatt
     1681 acgtcgccgg gcagataacg atagatttcc atattcagga aatcaaccat gaaattgaga
     1741 tgcaaaaaca aaggttcgta atttccgtgt ttctttcctt cgcctgtcag ttcgatcagg
     1801 gtttccagtt gctcccttaa tgaagaacct tcctgtgata tcagcgtttt tttcttgtta
     1861 tctatcttac ggtattgaaa tttatcggcc ggataaacgc cggcgaatag aagtttgaca
     1921 aggtcgttta cttcggtcac gttcagatgg aacggctgtg cggaccggct gtggatcatt
     1981 tggaaaatcg tgacgatgtt c
//
LOCUS       DAOPJL010000074         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_68808_length_1949_cov_6.280359, whole genome shotgun sequence.
ACCESSION   DAOPJL010000074 DAOPJL010000000
VERSION     DAOPJL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68808_length_1949_cov_6.280359"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLP02_06080"
     CDS             <1..549
                     /locus_tag="PLP02_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPF43044.1"
                     /translation="EGQSLGSGVVYRYDENTDTYYLITNHHVVEDGDNYKIVFCDGSK
                     VVGTLLGANEDVDIAVLSFRGTNLEYDIEVSPLGDSSQVEVGMIVLAAGNPEGYNFYG
                     SLTMGILSGLDRSLSNSTALCLQHDAPINPGNSGGPLYNLDGEVIGINVSKLVATDIE
                     GMGFSIPINTVKTIVNTIAPTN"
     gene            complement(605..796)
                     /gene="rpmB"
                     /locus_tag="PLP02_06085"
     CDS             complement(605..796)
                     /gene="rpmB"
                     /locus_tag="PLP02_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPF43045.1"
                     /translation="MPRVCAVTGKKFMSGNQRSHSMHATRKLWKANLQKVRIVDENGQ
                     TKRVYVSARAMRNGKINRA"
     gene            1195..>1949
                     /locus_tag="PLP02_06090"
     CDS             1195..>1949
                     /locus_tag="PLP02_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPF43046.1"
                     /translation="MDIAYLDGNMLKTMVFNGNIKLKENTDRINQLNVFPVPDGDTGS
                     NMSATMSAGANAIRNVEDESIGIVSKVLARGMLMGARGNSGVILSQLFNGMSTALKGY
                     DKVNTVEFAQALRKGVEQAYQAVINPVEGTILTVAREGADEAISVASDVRNFAELFDI
                     YLKELRESLERTPELLPILKEVGVIDSGGAGYIEVAQGMYDAILGNIYEEKETVKPAP
                     FDNRTIDLNDIHGGRDFGYCTEFIMQIEKMDEF"
BASE COUNT          545 a          428 c          462 g          514 t
ORIGIN      
        1 gaaggacaat ctttaggatc cggagtcgta tatcgttatg atgaaaacac cgacacttat
       61 tacttaatca ccaatcatca tgtcgttgaa gatggcgaca attataagat agtattctgt
      121 gacggaagca aagtcgtcgg aacattgctt ggagccaacg aggatgttga cattgccgtt
      181 ctgtcattca gaggaaccaa tttggaatac gatatcgaag tgtcgccctt gggggattct
      241 tcccaagtag aagtcggcat gatcgttcta gctgccggta accccgaagg atataatttc
      301 tacggttcgc ttactatggg aatcctgtcg ggacttgacc ggtcgcttag caattcgacg
      361 gcactgtgct tgcagcacga cgctccaatt aatcccggta acagcggcgg tccgctttat
      421 aatctggacg gcgaagtgat cggaatcaat gtcagcaagc ttgtcgctac ggatatcgag
      481 ggaatgggtt tctccattcc aatcaacacg gtaaaaacga tcgtcaacac catcgcaccg
      541 acaaactgat tacaaacaaa aggccctcgg atttccgagg gctttgtttt tggcagtttt
      601 tttgtcaggc tctgttgatt tttccgttcc tcatggctct agcagaaacg tacactctct
      661 tcgtctgccc gttttcgtca acgatccgga ctttctgaag attggctttc caaagttttc
      721 tggtcgcgtg catagaatgt gagcgctgat ttcctgacat gaatttcttt ccggttactg
      781 cgcatactct tggcatgata tcactccgtt tctgttttga tttcagcata tactatttta
      841 acatatctta taataaatgc aagcaaaaaa aacattttcc gaaagccgtc aaacaaaaga
      901 ccggcacctg gtattttttc ggtgaccgaa cgacaaaaaa aacaggcgat aggtaaaaaa
      961 tagacaaaaa ggtgccagtg tgtcactaat gaatcatatt agtgaaaact gaaaaccgat
     1021 tcacggattc tcgcgctttc cagccgatat gtatcgtaag taatctggtt gctttttctt
     1081 ttcggatgtg atagaatgtt attgcttttt gcgatttggc tagcaatgtc gggtaatatt
     1141 taatgcccat aagtaagata tatccgcatg ctcgaaatgc tttaggagga aatcatggac
     1201 atagcatacc ttgacgggaa tatgcttaaa acaatggtct tcaacggaaa cataaaacta
     1261 aaagaaaata ccgatagaat caatcaactg aacgtatttc ccgtaccgga cggagatacc
     1321 ggatcgaaca tgtcggccac gatgtcggcg ggagcgaatg ctatccgcaa cgtcgaggac
     1381 gaatcgatcg gaatcgtctc caaggtcctg gcccggggaa tgctcatggg agcgcggggt
     1441 aactctggcg ttattttgtc gcaactcttc aacggtatgt caaccgcttt gaaaggttac
     1501 gacaaggtta acactgtcga atttgcccag gcactcagaa aaggcgtgga acaagcttat
     1561 caggctgtca tcaatccggt tgaaggaacg atcctcaccg ttgcgagaga aggagcggac
     1621 gaagcgattt cggttgcatc cgatgtccgt aatttcgccg aactgtttga tatctatctg
     1681 aaagaattgc gcgaatccct ggaaagaact ccggagttgt tgccgattct gaaagaagtg
     1741 ggagtcatcg attccggcgg agccggctat atcgaagtcg cccagggaat gtacgatgcc
     1801 atcctcggca acatctacga ggaaaaggaa accgttaagc cggccccgtt tgacaacagg
     1861 acgattgatc tcaatgatat tcacggcggc cgtgatttcg gatattgcac cgaattcatc
     1921 atgcagatcg aaaaaatgga tgaattcaa
//
LOCUS       DAOPJL010000075         1910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_70849_length_1910_cov_7.592453, whole genome shotgun sequence.
ACCESSION   DAOPJL010000075 DAOPJL010000000
VERSION     DAOPJL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70849_length_1910_cov_7.592453"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLP02_06095"
     CDS             complement(<1..677)
                     /locus_tag="PLP02_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPF43047.1"
                     /translation="MIRIGIDAMGGDYAPKEQVEGTMLAIKNIPDIEVKLYGDPAEIS
                     KYLTNTERITIVPTRYVIGMGEKNPIKAIKEHPEGSMATMLASLRKDENDAVVSSGAT
                     QALIAGAHILVHRMQGFKRTAIAPVIPAISGKKTILLDTGANLEVRPEHMLQQAYFAY
                     VYSKEILGVEEPVVGLINIGTEDGKGREIDHEVFSLFKETNLFPFYGNVEPKSIFDPP
                     CDILISDG"
     gene            complement(714..>1910)
                     /locus_tag="PLP02_06100"
     CDS             complement(714..>1910)
                     /locus_tag="PLP02_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPF43048.1"
                     /translation="RLLQGDVGSGKTVCAAIAAVATISAKRQVAIMAPTEILAFQHYE
                     NFLAMFQKAKIDCETVFLSGSVKNPDRSEIMEKIKKVSPLVIIGTHALIQDATQFEKL
                     GLVIVDEQQRFGVDQRKALREKGITPDVLFLSATPIPRTLAITIFGDMDVSSIHTMPS
                     GRKKILSEIVSYDEMKKVYNLVSSELELGNQAYFIVPLIEKNDNSSLFSVEEFYAEIK
                     ENFPGELNTGLMHGKMKGEEKERILKDFADKKIRILVSTTVIEVGMDVKSATVMVVLN
                     ATRFGLSQLHQLRGRVGRSNKQSYCFFVVDDPSNDYERLSILEKTDDGFEISKADLEN
                     RGPGQVFGNEQSGIPGFQMANFVTDAFLLEKARDDVAKVASSKDELSQCLMKKALKSI
                     DTFTLD"
BASE COUNT          447 a          477 c          425 g          561 t
ORIGIN      
        1 ccgtcgctta tcaggatatc gcatggcgga tcgaagatcg acttcggttc gacattgcca
       61 taaaaaggaa acagattcgt ctccttgaaa agcgaaaaaa cttcgtgatc aatctcgcgg
      121 ccttttccat cctcggtacc tatgttgatg aggccgacaa cgggttcttc gactccgagg
      181 atctccttgg aatacacata agcgaaataa gcctgttgca acatatgttc cggtcttact
      241 tcaagattgg cacctgtatc caaaagaatc gtttttttgc cggatatggc aggaatcacc
      301 ggtgcgatcg ccgtgcgttt gaatccctgc atgcgatgaa cgagaatgtg ggctcccgcg
      361 atcaaagcct gggtggctcc actcgacacc acggcgtcgt tttcgtcttt tctgagactt
      421 gcgagcatgg tggccattga tccttccgga tgttccttga ttgctttgat cggattcttc
      481 tcgcccattc cgatcacata cctagtcgga acgatcgtta ttctttcggt attggtcaga
      541 tacttgctga tttccgccgg gtctccgtac agtttcactt cgatgtcagg aatattcttg
      601 attgccagca tcgttccttc cacttgttct ttcggggcgt aatccccgcc catggcgtcg
      661 attcctattc tgatcatttg tctcactcct gttatccttt agtatatcac aaatcaatcc
      721 aatgtgaatg tgtcgatcga tttcaaagct tttttcatca ggcattggct caattcatct
      781 ttagaagaag caactttggc gacatcatct ctggcctttt ccaaaagaaa ggcatctgtc
      841 acgaaattgg ccatttggaa tccgggtata ccggattgct catttccgaa aacttgtccg
      901 ggcccgcggt tttccagatc tgccttgctt atttcgaaac cgtcgtcggt tttttccaat
      961 atcgaaaggc gttcgtaatc gtttgaggga tcgtcgacaa caaagaaaca gtaggattgt
     1021 ttgtttgatc tgccgaccct gccacgcaat tgatgaagct gcgacaagcc gaaacgggtg
     1081 gcattcaata cgaccatcac ggttgcactt ttcacatcca tgccgacttc aattacggtc
     1141 gtggaaacga gtatccggat ttttttgtcc gcaaaatctt tcaggatccg ttctttctct
     1201 tcgcctttca ttttgccatg catcaatccg gtattaagtt cacccgggaa attctcctta
     1261 atttccgcat agaattcttc cacggaaaac aatgaagaat tgtcattttt ctcgattaga
     1321 ggcacaatga aataagcttg gttgccaagc tcaagttccg aagaaaccag attatacact
     1381 ttcttcattt catcataaga tacgatttcg gacagaatct ttttccttcc cgaaggcatg
     1441 gtatggatcg aactaacatc catatcgccg aaaatcgtga tcgccaaggt acgcggaatc
     1501 ggagtcgcgc tcaagaaaag gacatcggga gttatgcctt tttcccgcag ggcttttctt
     1561 tgatcgacac cgaagcgctg ctgttcgtcg acaattacca gaccgagttt ctcgaattgg
     1621 gtggcatcct ggatcagagc atgcgtgccg atgatgacaa gtggcgaaac ttttttgatt
     1681 ttctccatta tctcgcttcg atccgggttt ttgacactgc cggacaaaaa aaccgtctcg
     1741 caatctattt tcgctttttg gaacatggca aggaaattct cataatgttg gaaggcgagt
     1801 atttccgtcg gagccatgat cgcaacttgc ctcttcgcac tgatggtcgc gactgcggcg
     1861 atggcggcgc acacggtctt tccgctgccg acatcccctt gcagaaggcg
//
LOCUS       DAOPJL010000076         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_77982_length_1788_cov_8.998269, whole genome shotgun sequence.
ACCESSION   DAOPJL010000076 DAOPJL010000000
VERSION     DAOPJL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77982_length_1788_cov_8.998269"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLP02_06105"
     CDS             <1..903
                     /locus_tag="PLP02_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HPF43049.1"
                     /translation="RPDEVRLMLIDPKKVDLQQFAGIPHLVTPIIDELKLAVEALKWA
                     VQEMERRYETLKKFRVVNVKDYYSRRSDDPEFEVMPRIVIIVEEASDLLLSGGNDVEE
                     QILKLTQKSRAVGIHLLLATQRPSADILKGSIKANIPTRFAFRVPSSVDSSVVLDATG
                     AEKLLGKGDMLLSENGIIRRLQGAYLSPEEIESITDFITDQVMPEYLFTHESLVEAAK
                     TDASQAELDELFGEVARYVVSEDKCSLNKITQEFGVGFNRATQIVNTLELYGIVSSNA
                     GTKPREVLIGYERLEEILDELVRK"
     gene            900..1703
                     /gene="lepB"
                     /locus_tag="PLP02_06110"
     CDS             900..1703
                     /gene="lepB"
                     /locus_tag="PLP02_06110"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPF43050.1"
                     /translation="MIPDEDKKPPIDEKAEIAKILKRLPVRGVILWVLLAFIVLATII
                     LAEEPLYMFNFLESDPSIPLSGQIFRIFFYLCFLASSAYILWFCITYMFGAKKKKAAL
                     EGDSDSFKKRYSWFDILTVIPEFLVFVIIVNGFFLGFAYVDGYSMMDTYHDNQFVVID
                     HYNTTYEQGDVVIIQMTDSKWIKRLVAIPGDTLMVDDTGVYVNGVLADDNNRGAHISE
                     MVLPEGYYFVMGDNRDMADDSRDPAVGFIMFGQLLGKVVYPDPGQDWIR"
BASE COUNT          504 a          389 c          433 g          462 t
ORIGIN      
        1 cgtccggacg aagtcagact catgctgata gatcccaaaa aagtcgactt gcaacaattt
       61 gccggaatcc cacatctggt gacaccgatc atcgacgaac ttaaactcgc cgtggaagct
      121 ttgaaatggg cagtccagga aatggaaaga cgttatgaga ccctgaaaaa attccgggtt
      181 gtcaacgtca aggattacta tagcagaaga tccgatgatc ctgaattcga agtgatgccg
      241 cgtatcgtaa tcattgtcga agaagcatcc gatctattgc ttagcggcgg aaacgatgtc
      301 gaagaacaga ttctcaaact gacccagaaa tcccgtgccg taggaattca tctgcttctt
      361 gccactcaaa gaccttcagc tgatattctg aaaggttcaa ttaaggcaaa cattccaacc
      421 agatttgctt tccgggttcc gtcgtcggtt gactcatcag tcgtattaga tgcgaccggc
      481 gccgaaaaat tgcttggaaa aggcgatatg ctattatcgg aaaacggcat aataaggcgc
      541 cttcagggag cttatctgtc gcctgaggaa attgaatcca tcacggattt catcacagac
      601 caggtcatgc cggaatatct gtttacccac gaaagtctgg tcgaagcagc caagaccgat
      661 gcctcgcagg cggaacttga cgaactgttc ggtgaagttg ccagatatgt agtttcagaa
      721 gataagtgct ctttgaacaa aataacccag gaatttggag tcggcttcaa ccgggcaact
      781 cagatagtca acactttaga actttatgga attgtttcaa gcaacgccgg gaccaaaccg
      841 cgtgaagtct tgatcggata tgaaagactc gaggagattt tggacgagct ggtgagaaaa
      901 tgattccgga tgaagacaaa aaaccaccta ttgacgaaaa agctgagatt gcgaaaatac
      961 tgaaaagact gcctgtcagg ggagtgattc tctgggtctt gttggcattt attgttttag
     1021 ccaccatcat tttggccgaa gaacctctgt atatgttcaa ctttctcgaa tccgacccga
     1081 gcattccgct ttcgggtcag atttttcgta tattcttcta tctttgcttt ctggcgtctt
     1141 ccgcctacat cttgtggttt tgcataacat atatgtttgg cgccaaaaag aaaaaagccg
     1201 cgctcgaagg cgattcggat agtttcaaaa aaagatattc atggttcgat attctgacag
     1261 taattccaga gtttctggtt ttcgttatca tcgtgaacgg gttttttctg ggattcgcct
     1321 atgttgatgg atactcgatg atggatacct accatgacaa ccagtttgtc gtcatcgatc
     1381 attacaacac gacctacgag caaggcgatg tcgtcatcat ccaaatgacc gattctaaat
     1441 ggataaaacg tctggtggcg attccgggcg atacattgat ggtagacgac accggagttt
     1501 acgtgaacgg cgtgctggcg gacgataaca atcgtggcgc ccatatatcg gaaatggttt
     1561 tgccggaagg atattatttt gtcatgggag acaaccgcga catggcggac gattctcgcg
     1621 atcccgccgt cggattcatc atgtttgggc aattgctcgg caaagtagtg tatcccgatc
     1681 cgggacagga ttggatcagg tgattgttat gaatcaaata caatggtatc ccggccatat
     1741 ggccaaggcg aaaaaagaag taatcgaaaa gctgtcgcaa gtcgacat
//
LOCUS       DAOPJL010000077         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_85293_length_1682_cov_7.457283, whole genome shotgun sequence.
ACCESSION   DAOPJL010000077 DAOPJL010000000
VERSION     DAOPJL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85293_length_1682_cov_7.457283"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLP02_06115"
     CDS             <1..278
                     /locus_tag="PLP02_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPF43051.1"
                     /translation="IDGVEIYNPGTDTGIIAFNLKNVPSHDAVSFYAENGVDIRAGHH
                     CAQLVTKWLGIPSCLRASFYLYSDESDAIRLAEVTEKAVEYFQSLGF"
     gene            289..717
                     /locus_tag="PLP02_06120"
     CDS             289..717
                     /locus_tag="PLP02_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HPF43052.1"
                     /translation="MTRLDNLYREVIMEHYKNPRNKGLVGGEGFTVTHIKNPSCGDDI
                     TVETKVENGIVKACHHEGTGCSICCASASVMTEIMTGKKLEEAKNVADNYLKMIHNEP
                     FDESVDFEEAEAFSGVRQFPARTKCASIAWQAFQNTVKKE"
     gene            722..>1682
                     /gene="sufB"
                     /locus_tag="PLP02_06125"
     CDS             722..>1682
                     /gene="sufB"
                     /locus_tag="PLP02_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPF43053.1"
                     /translation="MPMKKDDKKNAGNIVTDYKYGFSTDAKTVFQTGKGLSEDVVRQI
                     SAYKKEPEWMTDIRMKAYQAFLKIKNPSWGPDLSRIDFDDITYYIKSSEKVEDEWDNV
                     PDKIKDTFDKLGIPEAEQKYLAGVTSQFESEAVYHSTIKELTDEGVIFLDTDSALKNY
                     PEIFRKYFGKAVSHTDNKYAALNTAVWSGGSFIYVPKGVIIKKPLQSYFRINTEKMGQ
                     FERTLIIVEDDASLNYVEGCTAPIYSNDSLHAAVVEIFVMNRAKCRYSTVQNWSNNII
                     NLVTKRAVVGDYGLMEWIDGNIGSGLNMKYPGCVLKGDHAKGTT"
BASE COUNT          496 a          410 c          416 g          360 t
ORIGIN      
        1 aaatcgacgg agtcgagatc tacaacccgg gaaccgacac cggcatcatc gccttcaacc
       61 tgaaaaacgt gccttcccac gacgcagttt ctttctatgc cgaaaacggc gtggacatca
      121 gagccggaca tcactgcgcc caactagtaa ccaaatggct tgggatacct tcatgtctca
      181 gggcttcttt ctatttgtac agcgacgaat cggatgccat ccgcctggcg gaagtgaccg
      241 aaaaagccgt cgaatatttc caaagtctcg gattctgagg tgaccatcat gacaagactt
      301 gacaatttat accgcgaagt gatcatggaa cattacaaga atcctcgcaa caagggtctc
      361 gtaggcggtg aaggattcac ggtaacgcat atcaagaatc cgagttgcgg cgatgatata
      421 acggtagaga caaaagtcga aaacggtatc gtcaaagcct gtcaccatga agggaccggc
      481 tgttcgatct gttgtgcctc ggcttcggtc atgacggaga taatgaccgg aaagaaattg
      541 gaagaagcaa agaacgtggc cgacaattat ttgaaaatga tccataacga accttttgac
      601 gaatcggtgg atttcgagga agccgaggca ttctccggcg ttcgccagtt ccctgcccgt
      661 actaaatgcg cgtcgatcgc ctggcaggcc ttccagaata ccgtgaaaaa agaataggta
      721 catgcccatg aaaaaagacg acaaaaaaaa cgccggtaac atcgttaccg actacaaata
      781 cggattttcg accgatgcca aaacggtttt tcagaccggc aaaggcttgt cggaggacgt
      841 tgtacgtcag atttccgcct acaagaaaga accggaatgg atgacagata tccgtatgaa
      901 ggcttatcag gcattcctga agatcaagaa tcctagctgg ggaccggatc tttcccggat
      961 cgatttcgat gatatcactt attatatcaa atcgtccgaa aaagtggagg acgaatggga
     1021 taatgttccg gacaagatca aggacacttt cgacaaactg gggattcccg aagccgagca
     1081 gaaatacctg gccggcgtca cgagccagtt cgaatcggaa gcggtttatc acagcacgat
     1141 caaggaactg accgacgaag gcgtcatatt tctcgacacg gacagcgccc tcaagaacta
     1201 tcccgagatt ttcagaaaat atttcggcaa agccgtgagt cataccgata acaagtatgc
     1261 cgccctgaat acggcggtat ggagcggcgg gtctttcatc tatgtcccta agggcgtcat
     1321 aatcaaaaag ccgctgcaat cctatttccg tatcaacacg gaaaaaatgg gacaattcga
     1381 acgaacgctg atcattgtcg aagacgacgc ttcgttaaac tatgtcgaag gctgcacggc
     1441 cccgatctat tcgaacgatt cccttcacgc agccgttgtc gagatctttg tgatgaaccg
     1501 ggcgaaatgt cgttattcaa cagttcagaa ctggtccaac aatatcatca atctggtgac
     1561 caaaagggcc gtagtgggtg attacggact catggagtgg atcgacggaa acatcggctc
     1621 cggcctcaac atgaaatacc ccggatgcgt ccttaagggt gatcacgcca aaggaacgac
     1681 ta
//
LOCUS       DAOPJL010000078         1577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3278
            NODE_93680_length_1577_cov_9.206965, whole genome shotgun sequence.
ACCESSION   DAOPJL010000078 DAOPJL010000000
VERSION     DAOPJL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865013
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:09:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,228
            CDSs (total)                      :: 1,181
            Genes (coding)                    :: 1,177
            CDSs (with protein)               :: 1,177
            Genes (RNA)                       :: 47
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93680_length_1577_cov_9.206965"
                     /isolate="AMR_MDS_3278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLP02_06130"
     CDS             <1..307
                     /locus_tag="PLP02_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPF43054.1"
                     /translation="KLGRVLGPKGLMPNPKTGTITQDITKAVSESKGGKITYRVDKGG
                     NIQAIVGKVSFKPEALVENIKAVTDVIMKAKPSTVKGVYLKNVAISSTMGPGVDIEI"
     misc_feature    304..429
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            450..941
                     /gene="rplJ"
                     /locus_tag="PLP02_06135"
     CDS             450..941
                     /gene="rplJ"
                     /locus_tag="PLP02_06135"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPF43055.1"
                     /translation="MSRETVIQAKQDAVVNIAEKMKKAKSIVLAEYRGLTVEKTEALR
                     KQLRKEGCEMCVIKNNISRRAAKETGYEALDQGLSGPNGVVFAYNDAVSAAKILFDFA
                     KKNPKLVLKSGIVDGDYYEPAEIKQIAQLPPRDILLAMLATQLYAPLRDLAVGLDLIS
                     NQE"
     gene            963..1337
                     /gene="rplL"
                     /locus_tag="PLP02_06140"
     CDS             963..1337
                     /gene="rplL"
                     /locus_tag="PLP02_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPF43056.1"
                     /translation="MAKITPEEFIASLKEMTILEIKALIDAMKNEFGIDPSAIAAAPA
                     QAAAAEEDQGPKEYDVVLTSCGQSKVSVIKVVREITGLGLIDAKKLVDEAPKAIKEKV
                     KEDEAKELQKKLQEAGATVDLK"
BASE COUNT          497 a          377 c          386 g          317 t
ORIGIN      
        1 caaactgggc cgcgtactcg gaccaaaagg tttgatgcca aacccgaaga ccggtacgat
       61 cacccaagac ataaccaaag ccgtatctga atccaagggg ggcaagatca cctaccgcgt
      121 tgacaagggc ggaaacatcc aggccatcgt tggaaaagta tcattcaaac cggaagcctt
      181 ggtagaaaac atcaaagccg tcacagatgt cattatgaag gcgaaacctt cgaccgtcaa
      241 aggcgtctat ttgaaaaacg tcgccatctc ctcaaccatg ggacctggtg tcgacatcga
      301 aatctgaata taaaaccttt atcaaagaca gtaggggcct aaagcttaat aatcctaccg
      361 agacaaagag aaagcaagtt ccgttcatgc ctcttttctc gcgagaaaag aggctttctt
      421 tttccaaata attgacaagg aggtaaacca tgtcgagaga aacagtcatc caggcgaaac
      481 aagacgcagt cgtgaatatc gccgaaaaga tgaaaaaagc aaaatccatc gtactagccg
      541 aataccgggg actgaccgtg gagaaaaccg aagcattgcg caaacagctc cgcaaggagg
      601 gctgcgaaat gtgcgtcatc aaaaacaaca tttcccgccg cgcagccaag gaaaccggtt
      661 atgaagcttt ggaccagggt ttgtccggtc caaacggagt ggtattcgca tacaacgatg
      721 ccgtttccgc agccaagatt ctgttcgatt tcgccaagaa aaatccgaaa cttgtgttga
      781 aatcgggaat cgtggacgga gattactacg aaccggcgga aatcaagcaa atcgcccaat
      841 tgccgccaag agacattttg ctagcaatgt tggcgacaca gctttacgcc ccgctaaggg
      901 atctggccgt cgggttggat cttatcagca accaagaata atctatttag gaggaaacca
      961 aaatggcaaa aattacacct gaagaattta tcgcatcctt gaaagaaatg acaatcctgg
     1021 agatcaaggc tttgatcgac gccatgaaga atgaattcgg tatcgatccg tccgccatcg
     1081 ccgccgcgcc cgcccaggcc gctgccgccg aagaagacca aggcccgaag gaatacgacg
     1141 tcgtcctcac aagttgtgga cagagcaagg tttcggtcat caaagttgtc cgcgaaatca
     1201 ccggactcgg cttgatcgat gccaagaaac tcgttgacga agctccgaaa gccatcaagg
     1261 aaaaagtcaa ggaagacgaa gccaaggaac tgcagaagaa attgcaggaa gccggagcca
     1321 ctgtcgacct gaaataaaaa agcgaaaacg aataaaacct gagaaaccct caggtttttg
     1381 ccgtttatgg gggtgaaagg aatggatcag tatttttccc ctgaaaatga cgccttgcca
     1441 tcaaaacgca ggacgttttt ttgccagata ggcgggatga attttgagtt tgtcaccgat
     1501 accggcgtct tttcaaaatc gggattggat tatggttcga gaacgcttct ggaagcgatc
     1561 ttgccggaaa tatccgg
//
